Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 581,056 A→G 100% T75A (ACA→GCA)  nohD → DLP12 prophage; DNA packaging protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913581,0560AG98.2% 5100.9 / 26.7 1998T75A (ACA→GCA) nohDDLP12 prophage; DNA packaging protein
Reads supporting (aligned to +/- strand):  ref base A (19/19);  new base G (795/1157);  total (821/1177)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 2000 total aligned reads displayed.

GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCG  >  NC_000913/580908‑581192
                                                                                                                                                    |                                                                                                                                        
    aTGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGa                                                                                                                                      <  8:298350/149‑1 (MQ=21)
    aTGCCCGTGCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTCTGACTCTGCCTCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGa                                                                                                                                      <  1:20244/149‑1 (MQ=11)
     tGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAt                                                                                                                                     <  6:30075/149‑1 (MQ=21)
       cccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtct                                                                                                                                   >  1:33933/1‑149 (MQ=21)
        ccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                                  >  5:7843/1‑149 (MQ=21)
        ccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                                  >  4:117257/1‑149 (MQ=21)
        ccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                                  <  4:145462/149‑1 (MQ=21)
         cGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATTGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTcc                                                                                                                                 <  8:142496/149‑1 (MQ=255)
          gtgcTGCGAGGCGGTGGCAAGGGTACTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTcca                                                                                                                                <  1:261243/146‑1 (MQ=255)
          gTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCATATCTcca                                                                                                                                >  2:44822/1‑149 (MQ=255)
          gTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                                  >  3:289573/1‑147 (MQ=21)
          gTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTcca                                                                                                                                <  6:13614/149‑1 (MQ=21)
          gTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTcca                                                                                                                                <  6:336317/149‑1 (MQ=21)
              tGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTccagc                                                                                                                              >  1:378216/1‑147 (MQ=21)
              tGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTccagc                                                                                                                              >  7:258678/1‑147 (MQ=21)
               gCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTccagccag                                                                                                                           >  4:293353/1‑149 (MQ=21)
                cGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGTAGGCCAGCGAGGCAGATCTccagccag                                                                                                                           >  8:188265/1‑148 (MQ=255)
                cGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTccagccag                                                                                                                           >  8:120438/1‑148 (MQ=21)
                cGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTccagccag                                                                                                                           <  5:39168/148‑1 (MQ=21)
                cGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTcaagccag                                                                                                                           <  2:207644/148‑1 (MQ=255)
                 gAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAgg                                                                                                                          >  4:355177/1‑148 (MQ=21)
                  aGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAggaa                                                                                                                        <  5:114266/149‑3 (MQ=19)
                   ggCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAc                                                                                                                       <  7:324528/149‑1 (MQ=255)
                    gCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAAAGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACt                                                                                                                      <  3:159763/149‑1 (MQ=255)
                     cGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTa                                                                                                                     <  5:156495/149‑1 (MQ=255)
                     cGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTa                                                                                                                     <  7:52211/149‑1 (MQ=255)
                     cGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTa                                                                                                                     <  3:62472/149‑1 (MQ=255)
                      ggTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAt                                                                                                                    >  3:35035/1‑149 (MQ=255)
                       gtgtCAAGGGTAATGAGGTGCTTTATGACTCTGCAGCAGTCATAAAATGTTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCTCCTGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGTAACTAtt                                                                                                                   <  6:72654/149‑1 (MQ=11)
                       gTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                                   <  5:337419/149‑1 (MQ=255)
                       gTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                                   >  1:131589/1‑149 (MQ=255)
                       gTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                                   <  8:61757/149‑1 (MQ=255)
                       gTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                                   <  3:244823/149‑1 (MQ=255)
                       gTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGGCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                                   <  3:88791/149‑1 (MQ=255)
                        tGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTg                                                                                                                  <  5:2420/149‑1 (MQ=255)
                          gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGTAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                                 <  5:360672/148‑1 (MQ=255)
                          gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                                 <  5:210102/148‑1 (MQ=255)
                          gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                                 <  6:262014/148‑1 (MQ=255)
                          gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                                 <  4:168327/148‑1 (MQ=255)
                          gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAg                                                                                                                >  7:393070/1‑149 (MQ=255)
                          gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAACCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                                 <  2:292084/148‑1 (MQ=255)
                           cAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAg                                                                                                                >  3:219590/1‑148 (MQ=255)
                            aaGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTa                                                                                                              <  4:230720/149‑1 (MQ=255)
                            aaGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTa                                                                                                              >  4:327472/1‑149 (MQ=255)
                            aaGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTa                                                                                                              >  5:96523/1‑149 (MQ=255)
                             aGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAc                                                                                                             <  2:117244/149‑1 (MQ=255)
                             aGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAc                                                                                                             <  8:393070/149‑1 (MQ=255)
                              gggTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAc                                                                                                             >  7:87506/1‑148 (MQ=255)
                               ggTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGATTACg                                                                                                            >  3:272201/1‑148 (MQ=255)
                                  aaTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAt                                                                                                                    >  7:120708/1‑137 (MQ=255)
                                   aTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTcc                                                                                                                                 <  6:190694/123‑1 (MQ=25)
                                   aTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACg                                                                                                        <  1:236490/148‑1 (MQ=255)
                                     gAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCa                                                                                                     >  6:160458/1‑149 (MQ=255)
                                     gAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCa                                                                                                     >  4:265567/1‑149 (MQ=255)
                                      aGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCAt                                                                                                    >  8:270198/1‑149 (MQ=255)
                                      aGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCAt                                                                                                    <  7:194855/149‑1 (MQ=255)
                                      aGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCAt                                                                                                    <  2:23287/149‑1 (MQ=255)
                                       ggTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATc                                                                                                   <  7:221155/149‑1 (MQ=255)
                                         tGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGa                                                                                                 >  6:364538/1‑149 (MQ=255)
                                          gCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGAc                                                                                                >  7:35425/1‑149 (MQ=255)
                                          gCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGAc                                                                                                >  3:136929/1‑149 (MQ=255)
                                           cTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGAc                                                                                                <  7:286010/148‑1 (MQ=255)
                                           cTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACt                                                                                               <  3:106616/149‑1 (MQ=255)
                                            tttATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACt                                                                                               >  8:367977/1‑148 (MQ=255)
                                              tATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTa                                                                                             <  8:168078/148‑1 (MQ=255)
                                              tATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAc                                                                                            >  1:100595/1‑149 (MQ=255)
                                               aTGACTCTGCCGGCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAcg                                                                                           >  1:319059/1‑149 (MQ=255)
                                               aTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAcg                                                                                           >  6:4874/1‑149 (MQ=255)
                                                tGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAcgc                                                                                          >  1:47561/1‑149 (MQ=255)
                                                tGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAcgc                                                                                          <  6:92302/149‑1 (MQ=255)
                                                 gACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCGGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAc                                                                                            <  2:152904/146‑1 (MQ=255)
                                                 gACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                                   >  7:167958/1‑123 (MQ=255)
                                                 gACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAcgcg                                                                                         >  5:185491/1‑149 (MQ=255)
                                                 gACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAcgcg                                                                                         >  6:217041/1‑149 (MQ=255)
                                                  aCTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGt                                                                                        <  2:253556/149‑1 (MQ=255)
                                                    tctGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTgc                                                                                      >  5:276334/1‑149 (MQ=255)
                                                    tctGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTgc                                                                                      >  2:17954/1‑149 (MQ=255)
                                                       gccgccGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACtt                                                                                              <  1:156625/138‑1 (MQ=255)
                                                        ccgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAATCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAg                                                                                  <  4:81710/149‑1 (MQ=255)
                                                        ccgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTgcgc                                                                                    <  2:376946/147‑1 (MQ=255)
                                                        ccgccgTCATAAAATGGTATGCCGAAAGGGACGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAg                                                                                  <  1:78982/149‑1 (MQ=255)
                                                         cgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                                  <  3:200649/100‑1 (MQ=255)
                                                         cgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAgg                                                                                 <  5:136349/149‑1 (MQ=255)
                                                         cgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAgg                                                                                 >  4:208779/1‑149 (MQ=255)
                                                         cgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAgg                                                                                 <  3:183996/149‑1 (MQ=255)
                                                          gccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                                   <  7:145460/114‑1 (MQ=255)
                                                          gccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGc                                                                                >  2:348009/1‑149 (MQ=255)
                                                           ccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGcc                                                                               <  5:303718/149‑1 (MQ=255)
                                                           ccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGcc                                                                               <  1:181675/149‑1 (MQ=255)
                                                            cgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGcc                                                                               >  2:317987/1‑148 (MQ=255)
                                                              tCATAAAATGGTATGCCTAAACGGATTCTTAAATTGAGAACGAAAAGCTGCAACGGGATGAATAAGAACTTCGCCACGCCAGCGAGGCAGATCTCAAGCCACGAACAATTAAGAACTAACGCCATCGAATTAAGCGTGCGCAGGCCGac                                                                            >  8:332433/1‑148 (MQ=2)
                                                              tCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGac                                                                            >  8:222812/1‑148 (MQ=12)
                                                              tCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGac                                                                            <  4:63393/149‑2 (MQ=12)
                                                               cATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGacg                                                                           >  8:253902/1‑147 (MQ=9)
                                                                aTAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAAc                                                                                                         >  4:184267/1‑118 (MQ=255)
                                                                aTAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGacg                                                                           >  1:295530/1‑146 (MQ=2)
                                                                aTAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGacg                                                                           >  2:76060/1‑146 (MQ=2)
                                                                aTAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGacg                                                                           >  3:317084/1‑146 (MQ=2)
                                                                        gTATGCCTAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCCGCCAGGGACTATTGAGTACGAACTCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGc                                                                  <  4:40273/149‑1 (MQ=255)
                                                                                          tGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCg                                                                                                  <  3:370382/99‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                        
GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCG  >  NC_000913/580908‑581192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 1200 total aligned reads displayed.
CAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTG  >  NC_000913/580906‑581187
                                                                                                                                                      |                                                                                                                                   
CAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGG                                                                                                                                     >  NB501016:230:HFVJLAFXY:2:11201:25523:11326/1‑151 (MQ=21)
CAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGG                                                                                                                                     >  NB501016:230:HFVJLAFXY:4:21405:11185:2451/1‑151 (MQ=21)
CAGGGAATGCCCGTTCTGCGAGGCGGGGGCAAGGGTAATGAGGTTCTTTAAGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGG                                                                                                                                     <  NB501016:230:HFVJLAFXY:4:21608:1094:3788/151‑1 (MQ=19)
 AGGGAATGCCCGTTCTGCGAGGCGGTTGCAAGGGTAATGAGGTGCTTTAGGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGACGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    <  NB501016:230:HFVJLAFXY:1:21209:12152:18757/151‑1 (MQ=19)
 AGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    <  NB501016:230:HFVJLAFXY:4:21511:15657:14736/151‑1 (MQ=21)
 AGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:1:21210:20041:18123/1‑151 (MQ=21)
  GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCA                                                                                                                                   <  NB501016:230:HFVJLAFXY:4:11501:23184:2549/151‑1 (MQ=21)
  GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:4:11401:10140:13480/1‑150 (MQ=21)
  GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:4:21606:24885:1698/1‑150 (MQ=21)
  GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACG‑AAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:1:21312:25445:7554/1‑149 (MQ=20)
  GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGGGCTTTATGACTCTGCCGCCATCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    <  NB501016:230:HFVJLAFXY:2:21210:11619:14030/150‑1 (MQ=20)
   GGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAG                                                                                                                                  <  NB501016:230:HFVJLAFXY:4:11509:6055:3041/151‑1 (MQ=21)
   GGAATGCCAGTTCTGCGATGCGGTGGCATGGGTAATGAGGTGCTTTATGACTCTTCCGCCGTCATAAAATGGTCTGCGGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAG                                                                                                                                  <  NB501016:230:HFVJLAFXY:1:21207:8499:1369/151‑1 (MQ=17)
      ATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATC                                                                                                                               <  NB501016:230:HFVJLAFXY:3:21512:11341:4117/151‑1 (MQ=20)
      ATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATC                                                                                                                               <  NB501016:230:HFVJLAFXY:3:11610:16748:5400/151‑1 (MQ=20)
      ATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATC                                                                                                                               <  NB501016:230:HFVJLAFXY:4:21402:10133:17457/151‑1 (MQ=20)
      ATGCCCGGTCTGTGAGGCTGTGGAAAGGGTTATGTGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATC                                                                                                                               <  NB501016:230:HFVJLAFXY:1:21107:6597:15270/151‑1 (MQ=17)
       TGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGTTCT                                                                                                                              >  NB501016:230:HFVJLAFXY:2:11306:8902:15767/1‑151 (MQ=20)
       TGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCT                                                                                                                              >  NB501016:230:HFVJLAFXY:3:11606:25336:11936/1‑151 (MQ=20)
        GCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTGATAAAATGGTATGCCGAAAGGGAGGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTGGAAGAACTGCGGCAGGCCAGCGAGGCAGATCT                                                                                                                              <  NB501016:230:HFVJLAFXY:1:11310:3868:3162/150‑1 (MQ=6)
        GCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTC                                                                                                                             >  NB501016:230:HFVJLAFXY:1:11209:26422:10539/1‑151 (MQ=20)
        GCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTC                                                                                                                             >  NB501016:230:HFVJLAFXY:1:11106:12287:9002/1‑151 (MQ=20)
         CCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAATAACTGCGGCAGGCCAGCGAGGCAGATCTCC                                                                                                                            <  NB501016:230:HFVJLAFXY:2:21312:18443:3720/151‑1 (MQ=20)
         CCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGTCAGGCCAGCGAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:4:21402:8640:3300/1‑151 (MQ=20)
         CCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:4:11409:3869:4622/1‑151 (MQ=20)
         CCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:3:21411:23723:5997/1‑151 (MQ=20)
         CCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGAGGCAGGCCAGCGAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:2:21208:21618:18978/1‑151 (MQ=20)
         CCCGTTCTGCGAGGCGGAGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGATGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAACAACTGCGGCAGGCCAGCGAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:1:21110:14285:15708/1‑151 (MQ=19)
          CCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCA                                                                                                                           <  NB501016:230:HFVJLAFXY:2:21205:20203:16603/151‑1 (MQ=20)
          CCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCA                                                                                                                           <  NB501016:230:HFVJLAFXY:1:11101:7003:19211/151‑1 (MQ=20)
          CCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:4:21410:10931:17447/1‑150 (MQ=20)
           CGTTCTGCGAGTCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:1:21202:16862:10613/151‑1 (MQ=20)
           CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:3:21606:13146:15478/151‑1 (MQ=20)
           CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:3:21610:16850:2372/151‑1 (MQ=20)
           CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:2:11106:20396:20014/151‑1 (MQ=20)
           CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:4:11411:25511:12994/151‑1 (MQ=20)
           CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:2:21209:18354:17456/1‑149 (MQ=20)
           CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:3:11401:24619:13414/1‑149 (MQ=20)
           CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCACCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCCGGAGGTTGAAGAACTGCGGCAGGACAGCTAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:3:21504:19531:20120/1‑149 (MQ=18)
           CGTGCTGCGAGGCGGTGGCAAGGGTACTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:1:21111:5895:9993/151‑1 (MQ=19)
           CGTGCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTTTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:4:11606:5420:8943/151‑1 (MQ=19)
            GTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGC                                                                                                                         >  NB501016:230:HFVJLAFXY:3:11503:5933:9475/1‑151 (MQ=20)
              TCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTCTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCC                                                                                                                        <  NB501016:230:HFVJLAFXY:3:11405:13767:8446/150‑1 (MQ=20)
               CTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCC                                                                                                                        >  NB501016:230:HFVJLAFXY:2:21102:11206:12337/1‑149 (MQ=20)
               CTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCC                                                                                                                        >  NB501016:230:HFVJLAFXY:4:11406:14155:9810/1‑149 (MQ=20)
               CTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTAATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCC                                                                                                                        >  NB501016:230:HFVJLAFXY:2:21112:19177:11273/1‑149 (MQ=20)
                 GCTAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCGGAAATTGATGCTGAAATTGAGAACTATAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGA                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11509:15270:5409/151‑1 (MQ=17)
                 GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTTCCTCCGTCATAAAATGGTATTCCGAAAGGGATGCTTAAATTTAGAACGAAAAGCTGCGCCGTGAGTTTGAAGAAATGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:1:11307:2498:18997/150‑1 (MQ=16)
                 GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAGATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     >  NB501016:230:HFVJLAFXY:4:11510:8520:9230/1‑150 (MQ=20)
                 GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGA                                                                                                                    <  NB501016:230:HFVJLAFXY:1:11303:24813:12614/151‑1 (MQ=20)
                 GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     >  NB501016:230:HFVJLAFXY:1:11203:21738:16296/1‑150 (MQ=20)
                 GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAG                                                                                                                      <  NB501016:230:HFVJLAFXY:2:11102:14363:11955/149‑1 (MQ=20)
                 GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCA                                                                                                                       <  NB501016:230:HFVJLAFXY:2:21305:22200:18705/148‑1 (MQ=21)
                 GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAGCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:3:11511:8154:12874/150‑1 (MQ=20)
                 GCGAGGCGGTGGCAAGGGTAATGACGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:1:21109:1676:9041/150‑1 (MQ=20)
                  CGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGA                                                                                                                    >  NB501016:230:HFVJLAFXY:1:11107:19993:1760/1‑150 (MQ=20)
                   GAGGCGGTGGCAAGTGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  <  NB501016:230:HFVJLAFXY:2:11104:20042:15286/151‑1 (MQ=20)
                   GAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  <  NB501016:230:HFVJLAFXY:2:11105:1214:12297/151‑1 (MQ=20)
                   GAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  <  NB501016:230:HFVJLAFXY:3:21509:19465:17652/151‑1 (MQ=20)
                   GAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  <  NB501016:230:HFVJLAFXY:3:11510:22650:16453/151‑1 (MQ=20)
                     GGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAAAGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTA                                                                                                                <  NB501016:230:HFVJLAFXY:2:11308:10064:6851/151‑1 (MQ=19)
                      GAGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAT                                                                                                               <  NB501016:230:HFVJLAFXY:2:11107:1769:4229/151‑1 (MQ=20)
                       GGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGATGAAATTGAGAACGAAAATCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATT                                                                                                              <  NB501016:230:HFVJLAFXY:4:11610:23099:1944/151‑1 (MQ=19)
                       CGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATT                                                                                                              <  NB501016:230:HFVJLAFXY:1:11110:22122:7426/151‑1 (MQ=20)
                       CGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATT                                                                                                              <  NB501016:230:HFVJLAFXY:2:11202:2070:8390/151‑1 (MQ=20)
                       CGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATT                                                                                                              <  NB501016:230:HFVJLAFXY:2:11212:1552:7706/151‑1 (MQ=20)
                        GGTGGGAAGGGTAATGAGGGTCTTTATGACTCTGCCGACGTCATAAAATGGTATGCCGAAATGGATGCTGAAATTGAGATCGAAATGCTGCGCCTGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:3:11604:23630:2587/151‑1 (MQ=16)
                        GGTGGCTAGGGTAATGTGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGGGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:3:21506:20230:15618/151‑1 (MQ=18)
                        GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTTCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:4:11404:4389:1041/151‑1 (MQ=19)
                        GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGACAGGGATGCTGAAATTGAGAACGAAAAGCTGCGACGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:3:11507:2052:5036/151‑1 (MQ=19)
                        GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:2:21311:19275:17340/151‑1 (MQ=20)
                        GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             >  NB501016:230:HFVJLAFXY:1:21104:16746:20163/1‑151 (MQ=20)
                        GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:2:11103:7769:18740/151‑1 (MQ=20)
                        GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:1:11306:11595:10078/151‑1 (MQ=20)
                        GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:2:11204:13387:3544/151‑1 (MQ=20)
                        GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGACTCCGTCATAAAATTGTATGCCGAAAGGGATGCGGAAATGGAGAAGGAAACGCTGCGCCGGGAGGGTGATGAACTGCGGCAGTCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:3:21605:14273:9581/151‑1 (MQ=15)
                        GGTGGCAAGGGTAATGAGGTGCGTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:3:11404:21391:3370/151‑1 (MQ=19)
                        GGGGGCATGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGATAGGGATGCTGAATTTGAGATCGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:3:21601:5214:2184/151‑1 (MQ=18)
                         GTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCC                                                                                                                        <  NB501016:230:HFVJLAFXY:4:21609:11288:2757/139‑1 (MQ=21)
                          TGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             >  NB501016:230:HFVJLAFXY:1:21310:17223:5875/1‑149 (MQ=20)
                          TGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          >  NB501016:230:HFVJLAFXY:1:11211:11000:9598/1‑136 (MQ=21)
                           GGCAAGTGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTTAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:3:11406:5202:19831/150‑1 (MQ=19)
                           GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGT                                                                                                          >  NB501016:230:HFVJLAFXY:4:21611:18488:15826/1‑151 (MQ=20)
                           GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGT                                                                                                          >  NB501016:230:HFVJLAFXY:2:11306:14530:5607/1‑151 (MQ=20)
                           GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGT                                                                                                          <  NB501016:230:HFVJLAFXY:4:21401:10277:5382/151‑1 (MQ=20)
                           GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGT                                                                                                          <  NB501016:230:HFVJLAFXY:1:11202:11607:5013/151‑1 (MQ=20)
                           GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:2:21101:13440:6453/150‑1 (MQ=20)
                           GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:4:11504:18432:7591/150‑1 (MQ=20)
                           GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:1:11103:2831:5397/150‑1 (MQ=20)
                           GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:2:21310:17404:19788/150‑1 (MQ=20)
                           GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTAAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:3:21607:11669:9974/150‑1 (MQ=19)
                            GCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTA                                                                                                         <  NB501016:230:HFVJLAFXY:1:11101:20327:7737/151‑1 (MQ=20)
                             CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAC                                                                                                        <  NB501016:230:HFVJLAFXY:1:21301:23874:10768/151‑1 (MQ=20)
                             CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAC                                                                                                        >  NB501016:230:HFVJLAFXY:4:21601:3896:5064/1‑151 (MQ=20)
                             CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAC                                                                                                        >  NB501016:230:HFVJLAFXY:4:21412:4559:3246/1‑151 (MQ=20)
                             CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAC                                                                                                        <  NB501016:230:HFVJLAFXY:2:11310:7849:6392/151‑1 (MQ=20)
                             CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAC                                                                                                        >  NB501016:230:HFVJLAFXY:2:21207:17706:13175/1‑151 (MQ=20)
                             CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAC                                                                                                        >  NB501016:230:HFVJLAFXY:4:11604:12141:10904/1‑151 (MQ=20)
                             CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATAGAGTAC                                                                                                        >  NB501016:230:HFVJLAFXY:1:21106:23982:13114/1‑151 (MQ=19)
                             CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:1:11108:22579:16963/138‑1 (MQ=21)
                              acgtgtgcctcttccgatctTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       <  NB501016:230:HFVJLAFXY:2:11211:17061:5555/131‑1 (MQ=21)
                              AAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATTGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCTGGTGGTTGAATAACTGCGGGAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       <  NB501016:230:HFVJLAFXY:2:11110:21099:18458/151‑1 (MQ=17)
                              AAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTTTGCCGAAAGGGTTGCTGAAATTTAGAACGAAAAGCTGCGCCGGGAGGTTGAATAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       <  NB501016:230:HFVJLAFXY:2:21212:5228:7146/151‑1 (MQ=18)
                              AAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGTACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       <  NB501016:230:HFVJLAFXY:4:11602:21371:1413/151‑1 (MQ=19)
                              AAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       <  NB501016:230:HFVJLAFXY:2:21308:4016:8656/151‑1 (MQ=20)
                              AAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       <  NB501016:230:HFVJLAFXY:1:21212:11323:4953/151‑1 (MQ=20)
                              AAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       <  NB501016:230:HFVJLAFXY:3:11405:5437:11004/151‑1 (MQ=20)
                              AAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:2:21109:18932:15475/137‑1 (MQ=21)
                               AGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCagtacggaagaagccttcactggt                                                                                                       >  NB501016:230:HFVJLAFXY:1:11311:6124:4529/1‑126 (MQ=22)
                               AGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       >  NB501016:230:HFVJLAFXY:4:11503:17302:13630/1‑150 (MQ=20)
                                GGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAA                                                                                                     <  NB501016:230:HFVJLAFXY:4:11609:25496:16312/151‑1 (MQ=20)
                                  GTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGTTGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACG                                                                                                   <  NB501016:230:HFVJLAFXY:4:11603:20545:10882/151‑1 (MQ=19)
                                  GTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACG                                                                                                   >  NB501016:230:HFVJLAFXY:4:11402:6718:3217/1‑151 (MQ=20)
                                  GTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACG                                                                                                   <  NB501016:230:HFVJLAFXY:1:11305:16400:8250/151‑1 (MQ=20)
                                  GTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACG                                                                                                   <  NB501016:230:HFVJLAFXY:1:21207:15030:14042/151‑1 (MQ=20)
                                    AATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCC                                                                                                 <  NB501016:230:HFVJLAFXY:2:21106:19919:11450/151‑1 (MQ=20)
                                    AATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCC                                                                                                 >  NB501016:230:HFVJLAFXY:3:11608:21245:4624/1‑151 (MQ=20)
                                     ATGAGGTGCTTTATGCCTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGACCGCC                                                                                                 >  NB501016:230:HFVJLAFXY:1:21309:24669:17628/1‑150 (MQ=19)
                                     ATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGCTCTCCAGCCAGGAACTATTGAGTACGAACGCC                                                                                                 >  NB501016:230:HFVJLAFXY:2:11209:7419:6103/1‑150 (MQ=19)
                                     ATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCA                                                                                                <  NB501016:230:HFVJLAFXY:2:21111:16915:3170/151‑1 (MQ=20)
                                     ATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGC                                                                                                                         >  NB501016:230:HFVJLAFXY:3:21612:14442:13311/1‑126 (MQ=22)
                                      TGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCAT                                                                                               >  NB501016:230:HFVJLAFXY:3:11408:20037:7456/1‑151 (MQ=20)
                                      TGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAG                                                                                                                                  >  NB501016:230:HFVJLAFXY:2:11304:5144:5476/1‑116 (MQ=23)
                                       GAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATC                                                                                              <  NB501016:230:HFVJLAFXY:1:11205:11341:3857/151‑1 (MQ=20)
                                       GAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       >  NB501016:230:HFVJLAFXY:4:21505:25365:13788/1‑142 (MQ=20)
                                       GAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGC                                                                                                                         <  NB501016:230:HFVJLAFXY:3:11605:18372:5089/124‑1 (MQ=22)
                                       GAGGTGCTTTATGACTCTGCCGCCGTAATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCAT                                                                                               <  NB501016:230:HFVJLAFXY:3:11603:26679:18150/150‑1 (MQ=7)
                                        AGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGTAACTATTGAGTACGAACGCCATCG                                                                                             <  NB501016:230:HFVJLAFXY:3:21403:12275:8170/151‑1 (MQ=19)
                                        AGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGCACGCCATCG                                                                                             >  NB501016:230:HFVJLAFXY:2:21203:7685:9857/1‑151 (MQ=19)
                                        AGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCG                                                                                             <  NB501016:230:HFVJLAFXY:4:11409:13743:13055/151‑1 (MQ=20)
                                         GGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGCACGCCATCG                                                                                             >  NB501016:230:HFVJLAFXY:1:21103:12240:3078/1‑150 (MQ=19)
                                         GGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGACAGGAACTATTGAGTACGAACGCCATCG                                                                                             >  NB501016:230:HFVJLAFXY:2:21107:23266:7417/1‑150 (MQ=19)
                                          ccattttatgacggcggcagaGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGCC                                                                                           >  NB501016:230:HFVJLAFXY:3:11409:22067:16467/22‑151 (MQ=16)
                                          GTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAACGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACG‑CATCGACT                                                                                          >  NB501016:230:HFVJLAFXY:1:11302:19983:7483/1‑151 (MQ=18)
                                          GTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCTTCGAC                                                                                           >  NB501016:230:HFVJLAFXY:1:21203:1702:3471/1‑151 (MQ=19)
                                          GTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGAC                                                                                           >  NB501016:230:HFVJLAFXY:4:21408:5676:20288/1‑151 (MQ=20)
                                          GTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGAC                                                                                           >  NB501016:230:HFVJLAFXY:3:21608:17407:19462/1‑151 (MQ=20)
                                           TGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGCTCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACT                                                                                          >  NB501016:230:HFVJLAFXY:3:21405:23364:15803/1‑151 (MQ=42)
                                            GCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGCGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTT                                                                                         >  NB501016:230:HFVJLAFXY:3:11506:1319:10701/1‑151 (MQ=19)
                                            GCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACT                                                                                          <  NB501016:230:HFVJLAFXY:1:21311:22029:1338/150‑1 (MQ=20)
                                             CTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGAGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTT                                                                                         >  NB501016:230:HFVJLAFXY:4:11608:22468:1995/1‑150 (MQ=19)
                                              TTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAC                                                                                       >  NB501016:230:HFVJLAFXY:2:11103:16675:12566/1‑151 (MQ=20)
                                              TTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAC                                                                                       >  NB501016:230:HFVJLAFXY:2:21105:1402:4846/1‑151 (MQ=20)
                                              TTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAC                                                                                       >  NB501016:230:HFVJLAFXY:2:11108:2538:7897/1‑151 (MQ=20)
                                                TATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGC                                                                                     >  NB501016:230:HFVJLAFXY:1:11104:16259:13167/1‑151 (MQ=20)
                                                TATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACG                                                                                      <  NB501016:230:HFVJLAFXY:3:11602:22508:6656/150‑1 (MQ=20)
                                                 ATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCG                                                                                    <  NB501016:230:HFVJLAFXY:4:21411:15944:8789/151‑1 (MQ=20)
                                                 ATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       >  NB501016:230:HFVJLAFXY:1:11310:12685:13083/1‑132 (MQ=21)
                                                 ATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGAGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCG                                                                                    >  NB501016:230:HFVJLAFXY:3:11605:8072:4610/1‑151 (MQ=19)
                                                  TGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGT                                                                                   >  NB501016:230:HFVJLAFXY:3:11611:7578:5223/1‑151 (MQ=20)
                                                  TGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:2:11112:14932:2957/125‑1 (MQ=21)
                                                  TGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACT                                                                                                                 <  NB501016:230:HFVJLAFXY:4:21512:1651:10258/121‑1 (MQ=21)
                                                   GACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTG                                                                                  <  NB501016:230:HFVJLAFXY:4:11407:9173:3494/151‑1 (MQ=20)
                                                   GACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTG                                                                                  <  NB501016:230:HFVJLAFXY:1:21307:13226:5096/151‑1 (MQ=20)
                                                   GACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           >  NB501016:230:HFVJLAFXY:3:11402:14262:8614/1‑126 (MQ=21)
                                                   GACTCTGCCGCCGGCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAA                                                                                                     <  NB501016:230:HFVJLAFXY:1:11112:21192:8595/132‑1 (MQ=20)
                                                    ACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGC                                                                                 <  NB501016:230:HFVJLAFXY:3:21603:15498:16528/151‑1 (MQ=20)
                                                    ACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATC                                                                                              >  NB501016:230:HFVJLAFXY:4:21507:22094:5519/1‑138 (MQ=20)
                                                     CTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAACAGCTGCGCCGAGAGGGTGAAGAACTGCGGCAGGCCCGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCG                                                                                >  NB501016:230:HFVJLAFXY:2:11302:18770:2948/1‑151 (MQ=18)
                                                     CTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGCTCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCG                                                                                >  NB501016:230:HFVJLAFXY:1:11207:11816:19824/1‑151 (MQ=19)
                                                     CTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCG                                                                                >  NB501016:230:HFVJLAFXY:2:11205:17685:3472/1‑151 (MQ=20)
                                                     CTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCG                                                                                >  NB501016:230:HFVJLAFXY:1:21304:19084:12387/1‑151 (MQ=20)
                                                     CTCTGCCGCCGTCATAAAAAGGGATGCGGAAACGGAAGATGAAATTTAGAACAAAAACCTGACGCGGGAGGGTAAAGAACTGCTGCAGTCCATCGAGGCAGATCTCCAACCAGGAAATATTGATTACTAACGCCATCGACatcagctttc                                                                                 >  NB501016:230:HFVJLAFXY:4:21603:12590:16870/1‑140 (MQ=12)
                                                       CTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGC                                                                               >  NB501016:230:HFVJLAFXY:2:11207:3198:14944/1‑150 (MQ=20)
                                                       CTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCG                                                                                <  NB501016:230:HFVJLAFXY:3:11412:23032:15040/149‑1 (MQ=20)
                                                       CTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCG                                                                                             >  NB501016:230:HFVJLAFXY:3:11403:15985:17884/1‑136 (MQ=21)
                                                       CTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCAGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCG                                                                                <  NB501016:230:HFVJLAFXY:2:21211:6230:12240/149‑1 (MQ=30)
                                                        TGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGC                                                                               >  NB501016:230:HFVJLAFXY:4:11509:12260:10023/1‑149 (MQ=20)
                                                         GCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGG                                                                            <  NB501016:230:HFVJLAFXY:3:11601:2545:13071/151‑1 (MQ=20)
                                                          CCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGC                                                                           >  NB501016:230:HFVJLAFXY:3:21404:11550:15365/1‑151 (MQ=20)
                                                          CCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGC                                                                           >  NB501016:230:HFVJLAFXY:2:21202:13269:7833/1‑151 (MQ=20)
                                                           CGCCGTCATAAAATTGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:4:11601:15529:19938/116‑1 (MQ=21)
                                                           CGCCGTCATAAAATGGTATGCCGAAAGTGATGCTGAAATTGACAACGAAAAGCTGCGCCGGGTGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCC                                                                          <  NB501016:230:HFVJLAFXY:2:21102:9014:17881/151‑1 (MQ=18)
                                                           CGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGTAGACCTCCAGCCAGGAACTATTGAGTACGAACGCCATCCACTTAAGCGTGCGCAGGCC                                                                          >  NB501016:230:HFVJLAFXY:4:21509:11895:18519/1‑151 (MQ=18)
                                                           CGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCC                                                                          <  NB501016:230:HFVJLAFXY:2:11309:3260:18233/151‑1 (MQ=20)
                                                           CGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCC                                                                          >  NB501016:230:HFVJLAFXY:2:21103:23209:11465/1‑151 (MQ=20)
                                                           CGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  >  NB501016:230:HFVJLAFXY:3:21505:21140:17146/1‑111 (MQ=22)
                                                           CGCAGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGGGCGCAGGCC                                                                          <  NB501016:230:HFVJLAFXY:1:11301:15138:9020/151‑1 (MQ=15)
                                                            GCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCG                                                                         >  NB501016:230:HFVJLAFXY:3:11505:11807:19657/1‑151 (MQ=20)
                                                             CCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCG                                                                         >  NB501016:230:HFVJLAFXY:4:21604:21547:13258/1‑150 (MQ=20)
                                                              CGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGAC                                                                       >  NB501016:230:HFVJLAFXY:1:11105:18414:19588/1‑151 (MQ=16)
                                                              CGTCATAAAATGGTATGCCGAAAGGGAGGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGAGCGCAGGCCGAC                                                                       <  NB501016:230:HFVJLAFXY:1:11212:1176:11599/151‑1 (MQ=15)
                                                               GTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACG                                                                      >  NB501016:230:HFVJLAFXY:1:21101:10228:7178/1‑151 (MQ=9)
                                                                 CATAAAATGTTATGCCGAAAGGGATGCTGAAATTGAGCACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGTCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGAGCAGGCCGACGC                                                                     >  NB501016:230:HFVJLAFXY:4:11412:18156:8803/1‑150 (MQ=1)
                                                                 CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGC                                                                     >  NB501016:230:HFVJLAFXY:2:21303:26757:6532/1‑150 (MQ=3)
                                                                 CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGC                                                                     >  NB501016:230:HFVJLAFXY:3:21604:10569:3443/1‑150 (MQ=3)
                                                                 CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGC                                                                     >  NB501016:230:HFVJLAFXY:2:21302:1146:6540/1‑150 (MQ=3)
                                                                 CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGC                                                                     >  NB501016:230:HFVJLAFXY:1:21205:12076:16581/1‑150 (MQ=3)
                                                                 CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGAGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGAcgcc                                                                    >  NB501016:230:HFVJLAFXY:1:11308:6457:17774/1‑147 (MQ=3)
                                                                  ATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGCTCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGCAC                                                                   >  NB501016:230:HFVJLAFXY:3:21409:13855:8613/1‑151 (MQ=0)
                                                                  ATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGCAC                                                                   >  NB501016:230:HFVJLAFXY:1:21302:6927:11756/1‑151 (MQ=0)
                                                                  ATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGCAC                                                                   >  NB501016:230:HFVJLAFXY:4:21406:5055:17168/1‑151 (MQ=0)
                                                                  ATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGCAC                                                                   >  NB501016:230:HFVJLAFXY:3:21503:11163:17383/1‑151 (MQ=0)
                                                                   TAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:4:11507:18466:17405/110‑1 (MQ=6)
                                                                    AAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGCACGCCATCGACTTACGCGTGCGCAGGCCGCCGCAC                                                                   >  NB501016:230:HFVJLAFXY:4:21503:15317:18192/1‑149 (MQ=1)
                                                                    AAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCC                                                                          <  NB501016:230:HFVJLAFXY:3:21508:22114:9635/142‑1 (MQ=3)
                                                                               CCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGG                                                                            >  NB501016:230:HFVJLAFXY:3:21401:10674:16829/1‑129 (MQ=0)
                                                                                 GAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAT                                                                                                               <  NB501016:230:HFVJLAFXY:4:11611:24049:15362/92‑1 (MQ=0)
                                                                                      GGATGCTGAAATTGAGATCGAAAAGCTGCGGAGGGAGGTTGAAGACCTGCGGCAGTCCAGAGAGGCATATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGG                                                                            <  NB501016:230:HFVJLAFXY:3:21407:19986:15144/122‑1 (MQ=0)
                                                                                                                       GGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGT              <  NB501016:230:HFVJLAFXY:4:11512:2290:6340/151‑1 (MQ=30)
                                                                                                                                                      |                                                                                                                                   
CAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTG  >  NC_000913/580906‑581187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑