Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,429,235 2 bp→CA 100% coding (187‑188/3363 nt) stfR → Rac prophage; putative tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,429,2350TC96.8% 3782.1 / 43.0 1371L63Q (TTG→CAG) stfRRac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base C (672/650);  total (697/674)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
*NC_0009131,429,2360TA96.8% 3938.2 / 63.6 1369L63Q (TTG→CAG) stfRRac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base T (24/20);  new base A (671/652);  total (696/673)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 1371 total aligned reads displayed.

GTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGG  >  NC_000913/1429097‑1429379
                                                                                                                                          ||                                                                                                                                               
                   aGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGCCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGcc                                                                                                                     >  3:213598/1‑149 (MQ=255)
                      tcaaaGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCAACCTGCAGGTTGACGGTTTTCCACCATCGCACGCCgg                                                                                                                   >  4:258493/3‑148 (MQ=255)
                      tGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGACGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCgg                                                                                                                   >  5:139201/1‑148 (MQ=255)
                        aaaGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGa                                                                                                                 <  7:73033/148‑1 (MQ=255)
                         aaGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGAcc                                                                                                               <  5:358723/149‑1 (MQ=255)
                         aaGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGAc                                                                                                                >  6:9986/1‑148 (MQ=255)
                           gccagaCGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACCGCATGGATGTGGAGTACGGTCAGTACAGTGTCCTCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAt                                                                                                             >  1:340498/6‑149 (MQ=255)
                            ccttaCGTAACTGCACCACGGTGGTGGTGAACACGGTGGTCTCAGAGAATCCGGATGAAGCCGGGCGTTACATCATTGATGTGGAGTACTGTCAGTACAGTGTCATCCTGCATGTTGACGGTTTTCCACCATCGCACGCCGTGACCAtc                                                                                                            <  8:167885/145‑2 (MQ=255)
                             cagaCGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTTCAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAtca                                                                                                           >  5:2819/4‑147 (MQ=255)
                             cagaCGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAtca                                                                                                           >  7:244541/4‑147 (MQ=255)
                             cagaCGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAtca                                                                                                           >  1:320470/4‑147 (MQ=255)
                              agaCGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAtcat                                                                                                          >  4:69910/3‑146 (MQ=255)
                                  gTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCCCCgtg                                                                                                      >  5:257835/1‑149 (MQ=255)
                                  gTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCgtg                                                                                                      >  5:48007/1‑149 (MQ=255)
                                   tAACAGCACCAGGGTGGTGGTGAACACGTTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGAGACCATCACCttgt                                                                                                     <  4:352037/149‑1 (MQ=255)
                                   tAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCgtgt                                                                                                     <  5:333969/149‑1 (MQ=255)
                                   tAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCgtgt                                                                                                     <  7:379789/149‑1 (MQ=255)
                                   tAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCgtgt                                                                                                     <  5:224206/149‑1 (MQ=255)
                                    aaCAGCACAACGGTGGTGGTGAAAACGGTGGGCTCAGAGAATCCGGATGAATCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTACACCATCGCACGCCGGGACCATCACAGTGTa                                                                                                    >  6:204635/1‑149 (MQ=255)
                                     aCAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTAt                                                                                                   >  4:318520/1‑149 (MQ=255)
                                     aCAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTAt                                                                                                   >  1:101534/1‑149 (MQ=255)
                                     aCAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTAt                                                                                                   >  7:352002/1‑149 (MQ=255)
                                      cAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATg                                                                                                  <  6:355229/149‑1 (MQ=255)
                                       aGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGa                                                                                                 <  3:229788/149‑1 (MQ=255)
                                           ccacGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTGCGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGAt                                                                                             <  8:2422/149‑1 (MQ=255)
                                           ccacGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGAt                                                                                             <  1:383432/149‑1 (MQ=255)
                                            cacGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCCTCCCCGTGTATGAAGAtt                                                                                            >  5:7267/1‑149 (MQ=255)
                                            cacGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGAtt                                                                                            <  6:144670/149‑1 (MQ=255)
                                             acGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtc                                                                                           <  4:162712/149‑1 (MQ=255)
                                             acGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtc                                                                                           <  3:112780/149‑1 (MQ=255)
                                                 tggtggtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACaa                                                                                       <  2:320189/149‑1 (MQ=255)
                                                   gtggtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAcc                                                                                     >  5:102300/1‑149 (MQ=255)
                                                      gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                  <  1:25303/149‑1 (MQ=255)
                                                      gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                  >  1:4427/1‑149 (MQ=255)
                                                        gAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCgggga                                                                                >  2:235383/1‑147 (MQ=255)
                                                         aaCACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtca                                                                                          <  8:343303/138‑2 (MQ=255)
                                                         aaCACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGAACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGAc                                                                               >  2:148924/1‑149 (MQ=255)
                                                            acGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATCGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCt                                                                            >  5:309734/1‑149 (MQ=255)
                                                            acGGTGGGCGCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCgg                                                                                                                   <  6:118636/110‑1 (MQ=255)
                                                             cggtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCgggga                                                                                <  5:91789/141‑3 (MQ=255)
                                                             cggtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTg                                                                           <  4:135361/146‑1 (MQ=255)
                                                              ggtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTg                                                                           >  3:53273/3‑148 (MQ=255)
                                                              ggtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGa                                                                          <  1:163489/147‑1 (MQ=255)
                                                               cTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGaa                                                                         <  8:316496/149‑1 (MQ=255)
                                                                  ggcTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACAGTGTATGAAGATTCACAACCGGGGACGCTGAATGa                                                                      <  8:277158/147‑1 (MQ=255)
                                                                   gcTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAt                                                                     <  2:51696/148‑1 (MQ=255)
                                                                    cTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGaat                                                                    >  1:91124/1‑147 (MQ=255)
                                                                    cTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtt                                                                    >  6:79155/1‑149 (MQ=255)
                                                                     tCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAttt                                                                   >  5:378777/1‑149 (MQ=255)
                                                                     tCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAttt                                                                   >  3:142746/1‑149 (MQ=255)
                                                                       agagAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTtc                                                                 >  8:107905/1‑149 (MQ=255)
                                                                       agagAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTtc                                                                 >  5:156456/1‑149 (MQ=255)
                                                                       agagAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGGCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtc                                                                                           <  2:378007/123‑1 (MQ=255)
                                                                       agagAATCCGGATGAAGCCGGGCGTTACAGCATGGAGGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCCCGCCGGGACCCTCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTtc                                                                 >  2:275031/1‑149 (MQ=255)
                                                                        gagaaTCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTtct                                                                <  3:252928/146‑1 (MQ=255)
                                                                            aTCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGGCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATTAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGt                                                            <  1:130322/149‑1 (MQ=255)
                                                                             tCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCt                                                                            >  7:211487/1‑132 (MQ=255)
                                                                             tCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCtgtg                                                           >  7:127525/1‑149 (MQ=255)
                                                                              ccGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCCCCATCGCACGCCGGGACCATCACCGTGTATGATGATTCACCACCGGGGACGCTGAATGATTTTCTATGTGc                                                          >  4:225342/1‑149 (MQ=255)
                                                                               cGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGCCGGTTTTCCACCATCACACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGc                                                          >  3:269784/1‑148 (MQ=255)
                                                                               cGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGc                                                          <  1:361737/148‑1 (MQ=255)
                                                                                ggATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCt                                                                            >  4:109116/1‑129 (MQ=255)
                                                                                ggATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCa                                                        >  1:202376/1‑149 (MQ=255)
                                                                                ggATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCa                                                        >  2:189355/1‑149 (MQ=255)
                                                                                 gATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACACCCGGGGACGCTGAATGATTTTCTCTGTGCCAt                                                       >  1:297891/1‑149 (MQ=255)
                                                                                   tGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACCACCGGGGACGCTGAATGATTTTCTCTGTGCCATGa                                                     >  6:293236/1‑149 (MQ=255)
                                                                                     aaGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCGGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAc                                                    <  8:258171/148‑1 (MQ=255)
                                                                                     aaGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAc                                                    <  1:225669/148‑1 (MQ=255)
                                                                                     aaGCCGGGCGTTACAGCATGGATGTAGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAc                                                    <  4:274469/148‑1 (MQ=255)
                                                                                      aGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACgg                                                  <  3:174470/149‑1 (MQ=255)
                                                                                        ccGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCCTCACCGTGTATGAAGATTCACAACCGGGGCCGCTGAATGATTTTCTCTGTGCCATGACggag                                                >  6:156473/1‑149 (MQ=255)
                                                                                              gTTACAGCATGAATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatgat                                          <  3:83873/149‑1 (MQ=255)
                                                                                                 aCAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGcc                                       >  6:57783/1‑149 (MQ=255)
                                                                                                  cAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGccc                                      >  7:265777/1‑149 (MQ=255)
                                                                                                    gCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCgg                                    >  8:204213/1‑148 (MQ=255)
                                                                                                    gCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCgg                                    <  7:47282/149‑2 (MQ=255)
                                                                                                     cATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGTCCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatgat                                          <  2:218986/142‑1 (MQ=255)
                                                                                                     cATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCggc                                   >  1:7095/1‑147 (MQ=255)
                                                                                                     cATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCggc                                   >  3:328880/1‑147 (MQ=255)
                                                                                                         gatgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGa                               <  4:39857/143‑1 (MQ=255)
                                                                                                         gatgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGa                               <  5:193345/143‑1 (MQ=255)
                                                                                                           tgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAgg                             >  7:170587/5‑149 (MQ=255)
                                                                                                            gtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggt                            >  7:300622/4‑148 (MQ=255)
                                                                                                              ggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgc                          >  7:328205/2‑146 (MQ=255)
                                                                                                              ggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgc                          >  3:355456/2‑146 (MQ=255)
                                                                                                               gAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgc                          <  2:339838/148‑4 (MQ=255)
                                                                                                               gAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgc                          <  6:378777/148‑4 (MQ=255)
                                                                                                               gAGTACGGGCAGTACAGGGTCATCCTGCAGGGTGACGGTTTTCCACCATCGCACGCCGGGACCAGCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgct                         <  2:116280/149‑5 (MQ=255)
                                                                                                                  tACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcg                      >  2:79578/1‑149 (MQ=255)
                                                                                                                    cGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgtc                    <  2:36273/149‑3 (MQ=255)
                                                                                                                     ggTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggt                            >  8:69911/1‑139 (MQ=255)
                                                                                                                     ggTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCg                   >  6:252353/1‑149 (MQ=255)
                                                                                                                      gTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgt                  >  7:85075/1‑149 (MQ=255)
                                                                                                                       tCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtc                 <  3:297829/149‑2 (MQ=255)
                                                                                                                       tCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtc                 <  6:338683/149‑2 (MQ=255)
                                                                                                                       tCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtc                 >  8:39493/1‑148 (MQ=255)
                                                                                                                         aGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtct                <  8:363528/148‑3 (MQ=255)
                                                                                                                         aGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGtctt               <  8:133502/149‑1 (MQ=255)
                                                                                                                           tACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgtc                    <  6:314360/142‑3 (MQ=255)
                                                                                                                           tACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTg              >  4:191497/1‑148 (MQ=255)
                                                                                                                                          ||                                                                                                                                               
GTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGG  >  NC_000913/1429097‑1429379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 245 total aligned reads displayed.
GTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGG  >  NC_000913/1429097‑1429379
                                                                                                                                          |                                                                                                                                                
GTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGAT                                                                                                                                      <  NB501016:230:HFVJLAFXY:1:11102:10955:1362/151‑1 (MQ=60)
   CAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAG                                                                                                                                         >  NB501016:230:HFVJLAFXY:2:21205:3280:9738/1‑145 (MQ=60)
      AACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGT                                                                                                                                              <  NB501016:230:HFVJLAFXY:1:21106:25596:15100/137‑1 (MQ=60)
       ACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCC                                                                                                                               >  NB501016:230:HFVJLAFXY:4:11510:14969:11580/1‑151 (MQ=60)
       ACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCC                                                                                                                               <  NB501016:230:HFVJLAFXY:4:11402:16572:15101/151‑1 (MQ=60)
              AATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACAT                                                                                                                        <  NB501016:230:HFVJLAFXY:2:21301:4934:14455/151‑1 (MQ=60)
                            CAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTAC                                                                                                          >  NB501016:230:HFVJLAFXY:1:11204:22564:11446/1‑151 (MQ=60)
                             AAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACC                                                                                                         >  NB501016:230:HFVJLAFXY:3:21412:18093:12986/1‑151 (MQ=60)
                                           CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           >  NB501016:230:HFVJLAFXY:1:21302:22816:7789/1‑151 (MQ=53)
                                           CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           <  NB501016:230:HFVJLAFXY:2:11208:19672:3163/151‑1 (MQ=53)
                                           CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           >  NB501016:230:HFVJLAFXY:4:11502:5431:13872/1‑151 (MQ=53)
                                           CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           >  NB501016:230:HFVJLAFXY:2:21108:13855:9874/1‑151 (MQ=53)
                                           CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           >  NB501016:230:HFVJLAFXY:2:21110:12583:11778/1‑151 (MQ=53)
                                           CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           >  NB501016:230:HFVJLAFXY:1:11210:22870:6771/1‑151 (MQ=53)
                                           CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           >  NB501016:230:HFVJLAFXY:4:11410:16679:4501/1‑151 (MQ=53)
                                           CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           <  NB501016:230:HFVJLAFXY:2:11101:19563:1297/151‑1 (MQ=53)
                                           CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           >  NB501016:230:HFVJLAFXY:3:11506:17812:3437/1‑151 (MQ=53)
                                            agccggtgactggagttcagacgtgctcttccgatctGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           <  NB501016:230:HFVJLAFXY:4:11404:10486:4231/113‑1 (MQ=24)
                                                GTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAC                                                                                      >  NB501016:230:HFVJLAFXY:4:21611:12515:11055/1‑151 (MQ=45)
                                                GTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAC                                                                                      >  NB501016:230:HFVJLAFXY:1:11101:20729:9816/1‑151 (MQ=45)
                                                GTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGGACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAAC                                                                                      <  NB501016:230:HFVJLAFXY:3:21603:20832:17650/151‑1 (MQ=60)
                                                GGGGTGGTGAAGACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTAGAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAC                                                                                      >  NB501016:230:HFVJLAFXY:2:11310:3975:20374/1‑151 (MQ=28)
                                                  GGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGGGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACC                                                                                     <  NB501016:230:HFVJLAFXY:2:11207:19876:7467/150‑1 (MQ=35)
                                                         AACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGC                                                                             >  NB501016:230:HFVJLAFXY:4:21509:11632:12384/1‑151 (MQ=60)
                                                           CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           >  NB501016:230:HFVJLAFXY:2:11105:16424:8237/1‑151 (MQ=31)
                                                           CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           >  NB501016:230:HFVJLAFXY:4:11503:5857:7462/1‑151 (MQ=31)
                                                           CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           <  NB501016:230:HFVJLAFXY:2:11309:17978:1286/151‑1 (MQ=31)
                                                           CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           <  NB501016:230:HFVJLAFXY:3:11601:12001:1833/151‑1 (MQ=31)
                                                           CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           <  NB501016:230:HFVJLAFXY:3:11604:13390:10317/151‑1 (MQ=31)
                                                           CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           <  NB501016:230:HFVJLAFXY:3:21404:12521:4584/151‑1 (MQ=31)
                                                           CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           >  NB501016:230:HFVJLAFXY:3:21405:10618:3723/1‑151 (MQ=31)
                                                           CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATCGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           >  NB501016:230:HFVJLAFXY:3:21511:12647:15827/1‑151 (MQ=24)
                                                            ACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           >  NB501016:230:HFVJLAFXY:2:21306:6412:8580/1‑150 (MQ=31)
                                                             CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAA                                                                         <  NB501016:230:HFVJLAFXY:1:11306:7051:8753/151‑1 (MQ=31)
                                                             CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                          >  NB501016:230:HFVJLAFXY:4:11406:6936:19814/1‑150 (MQ=31)
                                                             CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                          >  NB501016:230:HFVJLAFXY:2:11111:12017:12425/1‑150 (MQ=31)
                                                             CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                          >  NB501016:230:HFVJLAFXY:3:21408:14846:11844/1‑150 (MQ=31)
                                                             CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                          >  NB501016:230:HFVJLAFXY:2:11110:13058:9521/1‑150 (MQ=31)
                                                              GGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAT                                                                        <  NB501016:230:HFVJLAFXY:1:21107:7842:7743/151‑1 (MQ=31)
                                                              GGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAT                                                                        <  NB501016:230:HFVJLAFXY:3:21608:11268:7359/151‑1 (MQ=31)
                                                              GGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAT                                                                        >  NB501016:230:HFVJLAFXY:2:11203:26454:7442/1‑151 (MQ=31)
                                                              GGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAT                                                                        <  NB501016:230:HFVJLAFXY:1:11305:4385:11437/151‑1 (MQ=31)
                                                              GGTGGGCTCAGAGAAGCCGGATGAAGCCGGGCGTTTCAGCATGGATGTGGAGTACCGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAT                                                                        <  NB501016:230:HFVJLAFXY:2:21111:19658:2675/151‑1 (MQ=12)
                                                              GCTTGCCTCAGAAAAACCGGATGAAGCCGGGCGTTACAGCAGGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGAATCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAAT                                                                        <  NB501016:230:HFVJLAFXY:2:11111:5800:20091/151‑1 (MQ=60)
                                                               gtgggCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATG                                                                       <  NB501016:230:HFVJLAFXY:2:11102:21029:11704/146‑1 (MQ=33)
                                                                 GGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGCGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAT                                                                     <  NB501016:230:HFVJLAFXY:1:21210:25196:3283/151‑1 (MQ=28)
                                                                 GGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGA                                                                      <  NB501016:230:HFVJLAFXY:4:11610:22358:5302/150‑1 (MQ=33)
                                                                  GGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGCTT                                                                    >  NB501016:230:HFVJLAFXY:1:11201:7812:14011/1‑151 (MQ=32)
                                                                  GGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATT                                                                    >  NB501016:230:HFVJLAFXY:2:21109:8077:6751/1‑151 (MQ=36)
                                                                   gcgcagagAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACACCCGGGGACGCTGAATGATTT                                                                   <  NB501016:230:HFVJLAFXY:2:11102:21777:15669/143‑1 (MQ=29)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGCCCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   >  NB501016:230:HFVJLAFXY:3:11503:3851:15411/1‑151 (MQ=31)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   <  NB501016:230:HFVJLAFXY:4:11604:25337:11985/151‑1 (MQ=38)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   <  NB501016:230:HFVJLAFXY:1:21201:20714:5218/151‑1 (MQ=38)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   <  NB501016:230:HFVJLAFXY:3:11410:11485:19477/151‑1 (MQ=38)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   <  NB501016:230:HFVJLAFXY:1:21110:16311:12552/151‑1 (MQ=38)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   >  NB501016:230:HFVJLAFXY:4:11603:13362:14397/1‑151 (MQ=38)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   <  NB501016:230:HFVJLAFXY:1:21106:16192:10573/151‑1 (MQ=38)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   <  NB501016:230:HFVJLAFXY:2:21302:15753:3456/151‑1 (MQ=38)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACAGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   >  NB501016:230:HFVJLAFXY:4:11510:24578:7644/1‑151 (MQ=31)
                                                                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTGGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   <  NB501016:230:HFVJLAFXY:1:21306:24356:16318/151‑1 (MQ=45)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCCCGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:4:11509:10847:15841/1‑151 (MQ=32)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGCCCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:3:21503:12224:6351/1‑151 (MQ=32)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGCATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:2:11211:22424:17248/1‑151 (MQ=32)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:4:21410:10439:16792/1‑151 (MQ=39)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:2:11305:24691:5255/1‑151 (MQ=39)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:2:21212:23152:5570/1‑151 (MQ=39)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:2:21107:14244:11338/1‑151 (MQ=39)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:4:11412:17029:5275/1‑151 (MQ=39)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:3:11602:17515:15596/1‑151 (MQ=39)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:4:11409:6429:5801/1‑151 (MQ=39)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:1:21304:14623:17168/1‑151 (MQ=39)
                                                                    CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACAATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:1:21210:19639:3199/1‑151 (MQ=32)
                                                                    CTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTT                                                                  >  NB501016:230:HFVJLAFXY:1:11102:22950:9733/1‑151 (MQ=60)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACATCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTC                                                                 <  NB501016:230:HFVJLAFXY:4:21608:1863:2875/150‑1 (MQ=31)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                >  NB501016:230:HFVJLAFXY:3:11606:10971:4788/1‑151 (MQ=39)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                >  NB501016:230:HFVJLAFXY:3:21609:7248:13522/1‑151 (MQ=39)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                >  NB501016:230:HFVJLAFXY:3:21602:19221:13119/1‑151 (MQ=39)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                <  NB501016:230:HFVJLAFXY:4:11405:24553:14701/151‑1 (MQ=39)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                <  NB501016:230:HFVJLAFXY:4:21403:14134:2716/151‑1 (MQ=39)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTC                                                                 <  NB501016:230:HFVJLAFXY:4:11503:2199:7189/150‑1 (MQ=38)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTC                                                                 <  NB501016:230:HFVJLAFXY:3:11408:18450:3493/150‑1 (MQ=38)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTC                                                                 <  NB501016:230:HFVJLAFXY:3:11508:11513:16106/150‑1 (MQ=38)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTC                                                                 <  NB501016:230:HFVJLAFXY:3:21503:10047:18090/150‑1 (MQ=38)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATT                                                                    <  NB501016:230:HFVJLAFXY:1:11303:4333:12213/147‑1 (MQ=34)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                           >  NB501016:230:HFVJLAFXY:1:11206:14068:4656/1‑124 (MQ=32)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGGGTCATCCTGCAGTTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   <  NB501016:230:HFVJLAFXY:3:21604:12167:19960/148‑1 (MQ=28)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCACTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                >  NB501016:230:HFVJLAFXY:1:21106:3106:2812/1‑151 (MQ=32)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGGCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                <  NB501016:230:HFVJLAFXY:1:21302:23945:9415/151‑1 (MQ=32)
                                                                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGGGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCGGAATGATTTTCT                                                                <  NB501016:230:HFVJLAFXY:3:21506:19567:4789/151‑1 (MQ=25)
                                                                       AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                               <  NB501016:230:HFVJLAFXY:2:21308:5485:13926/151‑1 (MQ=40)
                                                                       AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                               <  NB501016:230:HFVJLAFXY:1:11101:23779:13852/151‑1 (MQ=40)
                                                                       AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                               <  NB501016:230:HFVJLAFXY:4:11501:8778:3576/151‑1 (MQ=40)
                                                                       AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                               <  NB501016:230:HFVJLAFXY:1:21206:25212:10574/151‑1 (MQ=40)
                                                                       AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGAGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                               <  NB501016:230:HFVJLAFXY:4:11510:3897:17042/151‑1 (MQ=40)
                                                                        GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACCGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCCTCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                              >  NB501016:230:HFVJLAFXY:1:11203:12483:14166/1‑151 (MQ=27)
                                                                        GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                              <  NB501016:230:HFVJLAFXY:2:11311:12299:18596/151‑1 (MQ=40)
                                                                        GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                              <  NB501016:230:HFVJLAFXY:4:11502:19749:9583/151‑1 (MQ=40)
                                                                        GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                              >  NB501016:230:HFVJLAFXY:4:11405:5953:19439/1‑151 (MQ=40)
                                                                        GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                              <  NB501016:230:HFVJLAFXY:3:21612:8714:3294/151‑1 (MQ=40)
                                                                        GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                              <  NB501016:230:HFVJLAFXY:1:21110:3841:5639/151‑1 (MQ=40)
                                                                        GAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCG                                                              >  NB501016:230:HFVJLAFXY:3:11609:21342:18868/1‑151 (MQ=60)
                                                                         AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTTGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCAGCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                             <  NB501016:230:HFVJLAFXY:3:11406:22385:5489/151‑1 (MQ=40)
                                                                         AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                             <  NB501016:230:HFVJLAFXY:2:11209:14698:16651/151‑1 (MQ=40)
                                                                         AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                             <  NB501016:230:HFVJLAFXY:1:11109:12504:4349/151‑1 (MQ=40)
                                                                         AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                             <  NB501016:230:HFVJLAFXY:3:11501:15592:18779/151‑1 (MQ=40)
                                                                         AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           >  NB501016:230:HFVJLAFXY:4:21409:19413:7135/1‑137 (MQ=30)
                                                                         AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTACGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                             <  NB501016:230:HFVJLAFXY:4:11401:25033:14153/151‑1 (MQ=33)
                                                                          GAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGT                                                            >  NB501016:230:HFVJLAFXY:1:21307:8876:5050/1‑151 (MQ=40)
                                                                          GAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGT                                                            <  NB501016:230:HFVJLAFXY:3:21605:2094:2037/151‑1 (MQ=40)
                                                                          GAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGAGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGT                                                            >  NB501016:230:HFVJLAFXY:4:21612:15589:12701/1‑151 (MQ=33)
                                                                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                           <  NB501016:230:HFVJLAFXY:1:11311:22565:14559/151‑1 (MQ=40)
                                                                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                           <  NB501016:230:HFVJLAFXY:4:21605:11456:10464/151‑1 (MQ=40)
                                                                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                           <  NB501016:230:HFVJLAFXY:3:11605:8603:15290/151‑1 (MQ=40)
                                                                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                           <  NB501016:230:HFVJLAFXY:1:21104:15407:6162/151‑1 (MQ=40)
                                                                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                           <  NB501016:230:HFVJLAFXY:4:11412:9805:16078/151‑1 (MQ=40)
                                                                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGAACGCTGAATGATTTTCTCTGTG                                                           <  NB501016:230:HFVJLAFXY:2:21207:19975:1916/151‑1 (MQ=40)
                                                                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGGCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATTAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                           <  NB501016:230:HFVJLAFXY:1:11212:18245:6552/151‑1 (MQ=27)
                                                                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGGCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                           <  NB501016:230:HFVJLAFXY:1:11106:11577:14295/151‑1 (MQ=33)
                                                                           AATCCGGATGAAGCCGGGCGTTACAGCATGGAGGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                           <  NB501016:230:HFVJLAFXY:1:11308:22444:14936/151‑1 (MQ=40)
                                                                            ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCCCCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                          >  NB501016:230:HFVJLAFXY:3:11408:14627:4136/1‑151 (MQ=39)
                                                                            ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                          >  NB501016:230:HFVJLAFXY:4:11510:4201:17245/1‑151 (MQ=39)
                                                                            ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                          >  NB501016:230:HFVJLAFXY:3:11409:19488:17341/1‑151 (MQ=39)
                                                                            ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                          >  NB501016:230:HFVJLAFXY:3:11605:12249:5270/1‑151 (MQ=39)
                                                                            ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                           >  NB501016:230:HFVJLAFXY:4:21402:20093:3370/1‑134 (MQ=29)
                                                                             cgCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                         <  NB501016:230:HFVJLAFXY:2:11202:4921:17862/149‑1 (MQ=36)
                                                                             TCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                         >  NB501016:230:HFVJLAFXY:2:11202:19615:18118/1‑151 (MQ=39)
                                                                             TCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                          <  NB501016:230:HFVJLAFXY:1:21101:9462:5158/150‑1 (MQ=38)
                                                                             TCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                   >  NB501016:230:HFVJLAFXY:4:21408:14946:19257/1‑141 (MQ=33)
                                                                              CCGGATGAAGCCGGGCGTTATAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                         <  NB501016:230:HFVJLAFXY:4:11405:23501:12711/150‑1 (MQ=31)
                                                                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCCTCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCA                                                        <  NB501016:230:HFVJLAFXY:3:21509:18688:5244/151‑1 (MQ=32)
                                                                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGCCGGTTTTCCACCATCACACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                         >  NB501016:230:HFVJLAFXY:2:21205:10805:16504/1‑150 (MQ=38)
                                                                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCA                                                        <  NB501016:230:HFVJLAFXY:1:11303:22311:14617/151‑1 (MQ=39)
                                                                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                         <  NB501016:230:HFVJLAFXY:1:21306:15294:8836/150‑1 (MQ=38)
                                                                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                         >  NB501016:230:HFVJLAFXY:2:21210:22033:13801/1‑150 (MQ=38)
                                                                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                         >  NB501016:230:HFVJLAFXY:4:11407:9592:4485/1‑150 (MQ=38)
                                                                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGGATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                         <  NB501016:230:HFVJLAFXY:1:21109:14166:3679/150‑1 (MQ=31)
                                                                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                       >  NB501016:230:HFVJLAFXY:4:21508:17114:10684/1‑151 (MQ=39)
                                                                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                       >  NB501016:230:HFVJLAFXY:4:11402:13202:13745/1‑151 (MQ=39)
                                                                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                       <  NB501016:230:HFVJLAFXY:4:21405:7081:11791/151‑1 (MQ=39)
                                                                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                       <  NB501016:230:HFVJLAFXY:2:11206:16898:7762/151‑1 (MQ=39)
                                                                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                       >  NB501016:230:HFVJLAFXY:1:21101:4575:9154/1‑151 (MQ=39)
                                                                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACAGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                       >  NB501016:230:HFVJLAFXY:2:11109:11737:19745/1‑151 (MQ=32)
                                                                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGACGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                       >  NB501016:230:HFVJLAFXY:2:21306:5827:11176/1‑151 (MQ=32)
                                                                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGGTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                       >  NB501016:230:HFVJLAFXY:1:11212:4279:8408/1‑151 (MQ=32)
                                                                                GGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACACCCGGGGACGCTGAATGATTTTCTCTGTGCCATG                                                      >  NB501016:230:HFVJLAFXY:1:21206:15694:6593/1‑151 (MQ=32)
                                                                                GGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATG                                                      >  NB501016:230:HFVJLAFXY:1:21303:11422:13560/1‑151 (MQ=39)
                                                                                GGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCGGAATGATTTTCTCTGTGCCATG                                                      <  NB501016:230:HFVJLAFXY:3:21405:17068:5740/151‑1 (MQ=32)
                                                                                GGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACAATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                         >  NB501016:230:HFVJLAFXY:1:11111:9474:7580/1‑148 (MQ=28)
                                                                                  ATGAAGCCGGGCGTTACAGCATGGATGTTGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGGTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAC                                                    <  NB501016:230:HFVJLAFXY:3:11607:24699:16725/151‑1 (MQ=47)
                                                                                  ATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAC                                                    <  NB501016:230:HFVJLAFXY:3:11611:12030:10451/151‑1 (MQ=39)
                                                                                  ATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAC                                                    >  NB501016:230:HFVJLAFXY:1:21106:15795:6727/1‑151 (MQ=39)
                                                                                  ATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGAACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAC                                                    >  NB501016:230:HFVJLAFXY:3:21512:5977:15482/1‑151 (MQ=32)
                                                                                   TGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACG                                                   >  NB501016:230:HFVJLAFXY:3:11602:24328:15634/1‑151 (MQ=39)
                                                                                   TGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACG                                                   >  NB501016:230:HFVJLAFXY:1:11201:26297:10346/1‑151 (MQ=39)
                                                                                    GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:2:21303:22721:15364/1‑151 (MQ=39)
                                                                                    GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                  <  NB501016:230:HFVJLAFXY:3:21503:15774:5234/151‑1 (MQ=39)
                                                                                    GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACG                                                   <  NB501016:230:HFVJLAFXY:4:21604:1795:4662/150‑1 (MQ=38)
                                                                                    GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACG                                                   <  NB501016:230:HFVJLAFXY:1:21105:14575:5696/150‑1 (MQ=38)
                                                                                    GAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:2:11108:17346:14621/1‑151 (MQ=60)
                                                                                     AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGCCCATCACCGTGTATGAAGATTCACAACCGGGGACGCGGAATGATTTTCTCTGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:3:21609:2612:4191/1‑150 (MQ=24)
                                                                                     AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:4:11405:2316:10093/1‑150 (MQ=38)
                                                                                     AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:1:11211:10706:8602/1‑150 (MQ=38)
                                                                                     AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:4:21608:1259:15009/1‑150 (MQ=38)
                                                                                     AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                >  NB501016:230:HFVJLAFXY:3:11406:15399:15539/1‑136 (MQ=27)
                                                                                     AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATT                                                                    >  NB501016:230:HFVJLAFXY:1:21310:10093:16374/1‑132 (MQ=22)
                                                                                     AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGAATCACAACCGGGCACGCTGAATGATTTTCTCTGAGCCATGACCG                                                  >  NB501016:230:HFVJLAFXY:3:21612:16794:5767/1‑150 (MQ=21)
                                                                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                <  NB501016:230:HFVJLAFXY:4:21409:20939:12187/151‑1 (MQ=39)
                                                                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                <  NB501016:230:HFVJLAFXY:2:11303:18635:2469/151‑1 (MQ=39)
                                                                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                <  NB501016:230:HFVJLAFXY:4:21411:22917:11511/151‑1 (MQ=39)
                                                                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:1:11203:20262:16968/1‑149 (MQ=36)
                                                                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:3:21406:5453:19576/1‑149 (MQ=36)
                                                                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:2:21305:4650:15597/1‑149 (MQ=36)
                                                                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                  >  NB501016:230:HFVJLAFXY:3:11610:18230:2428/1‑149 (MQ=36)
                                                                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                       >  NB501016:230:HFVJLAFXY:4:21506:16759:18615/1‑144 (MQ=30)
                                                                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGA                                                                      >  NB501016:230:HFVJLAFXY:2:21110:5432:8238/1‑129 (MQ=18)
                                                                                       GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCTCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                               <  NB501016:230:HFVJLAFXY:1:11102:10709:19629/151‑1 (MQ=39)
                                                                                       GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                               <  NB501016:230:HFVJLAFXY:3:11408:10988:19980/151‑1 (MQ=39)
                                                                                       GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                               <  NB501016:230:HFVJLAFXY:2:21305:12106:6212/151‑1 (MQ=39)
                                                                                       GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                               <  NB501016:230:HFVJLAFXY:4:21607:22325:5971/151‑1 (MQ=39)
                                                                                        CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAATATTCACAACCCGGGACGCAGAATGATTTTCTCTGTGCCATGACGCAGG                                               >  NB501016:230:HFVJLAFXY:4:11606:21059:14280/1‑150 (MQ=25)
                                                                                        CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGA                                              <  NB501016:230:HFVJLAFXY:2:11202:23015:13708/151‑1 (MQ=39)
                                                                                        CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGA                                              <  NB501016:230:HFVJLAFXY:4:21601:13960:13705/151‑1 (MQ=39)
                                                                                        CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                               >  NB501016:230:HFVJLAFXY:4:21609:24135:5552/1‑150 (MQ=38)
                                                                                        CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAC                                                    <  NB501016:230:HFVJLAFXY:4:11511:23497:13160/145‑1 (MQ=31)
                                                                                            GCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGAT                                          <  NB501016:230:HFVJLAFXY:1:11203:17781:9119/151‑1 (MQ=42)
                                                                                            GCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGAT                                          <  NB501016:230:HFVJLAFXY:4:11607:10868:9091/151‑1 (MQ=42)
                                                                                             CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                         >  NB501016:230:HFVJLAFXY:1:11307:9102:11192/1‑151 (MQ=43)
                                                                                             CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                         <  NB501016:230:HFVJLAFXY:2:11308:25001:19209/151‑1 (MQ=43)
                                                                                             CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                         <  NB501016:230:HFVJLAFXY:1:11107:19305:13527/151‑1 (MQ=43)
                                                                                             CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                         >  NB501016:230:HFVJLAFXY:4:11404:15501:9305/1‑151 (MQ=43)
                                                                                             CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                         <  NB501016:230:HFVJLAFXY:1:21304:8285:2360/151‑1 (MQ=43)
                                                                                              GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGC                                        <  NB501016:230:HFVJLAFXY:4:11412:15902:15536/151‑1 (MQ=45)
                                                                                              GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGC                                        <  NB501016:230:HFVJLAFXY:4:21511:20423:5758/151‑1 (MQ=45)
                                                                                              GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGC                                        <  NB501016:230:HFVJLAFXY:1:11303:18955:17168/151‑1 (MQ=45)
                                                                                              GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGAGGC                                        >  NB501016:230:HFVJLAFXY:3:21609:8619:9568/1‑151 (MQ=40)
                                                                                                 ACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCG                                     <  NB501016:230:HFVJLAFXY:3:21612:14014:10109/151‑1 (MQ=60)
                                                                                                     CATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGAT                                             >  NB501016:230:HFVJLAFXY:3:21607:25995:14169/1‑139 (MQ=60)
                                                                                                           CGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTC                                                                 <  NB501016:230:HFVJLAFXY:2:21112:17941:11842/113‑1 (MQ=60)
                                                                                                                    CGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACG                                                   >  NB501016:230:HFVJLAFXY:3:21606:14987:5447/1‑118 (MQ=60)
                                                                                                                        CAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTG              >  NB501016:230:HFVJLAFXY:3:21612:23237:19978/1‑151 (MQ=60)
                                                                                                                                          |                                                                                                                                                
GTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGG  >  NC_000913/1429097‑1429379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑