Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,429,252 G→A 100% P68P (CCG→CCA)  stfR → Rac prophage; putative tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,429,2520GA97.1% 4052.1 / 40.1 1474P68P (CCG→CCA) stfRRac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base G (24/20);  new base A (705/710);  total (743/731)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 1477 total aligned reads displayed.

CTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGT  >  NC_000913/1429105‑1429400
                                                                                                                                                   |                                                                                                                                                    
             cTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCgg                                                                                                                                        >  5:277036/1‑149 (MQ=255)
             cTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCgg                                                                                                                                        <  6:312020/149‑1 (MQ=255)
                    ccagaCGTAACAGCACCACGGTGGTGGTGAATACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAt                                                                                                                                  >  8:172118/5‑148 (MQ=255)
                     cagaCGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAtca                                                                                                                                >  5:186098/4‑147 (MQ=255)
                     cagaCGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAtca                                                                                                                                >  1:320470/4‑147 (MQ=255)
                     cagaCGTAACAGCACCACGGTGGTGGGGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAtca                                                                                                                                <  6:52141/146‑3 (MQ=255)
                        aCGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCg                                                                                                                             <  7:244053/149‑1 (MQ=255)
                         cGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCgt                                                                                                                            <  2:87272/149‑1 (MQ=255)
                             aCAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCgtgt                                                                                                                          >  2:2385/1‑147 (MQ=255)
                             aCAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTa                                                                                                                         <  1:198209/148‑1 (MQ=255)
                             aCAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTa                                                                                                                         <  8:141793/148‑1 (MQ=255)
                             aCAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTAt                                                                                                                        >  1:101534/1‑149 (MQ=255)
                             aCAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTAt                                                                                                                        <  3:272046/149‑1 (MQ=255)
                                  accacGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGa                                                                                                                   <  4:272177/149‑1 (MQ=255)
                                   ccacGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTGCGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGAt                                                                                                                  <  8:2422/149‑1 (MQ=255)
                                    cacGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCAtc                                                                                                                                 >  4:184334/1‑132 (MQ=255)
                                    cacGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGAtt                                                                                                                 <  2:385158/149‑1 (MQ=255)
                                            tggtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccg                                                                                                         <  3:252839/149‑2 (MQ=255)
                                             ggtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGCCCATCACCGTGTATGAAGATTCACaagcgg                                                                                                        >  2:369283/1‑145 (MQ=255)
                                              gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCgg                                                                                                                                        >  3:148907/1‑116 (MQ=255)
                                              gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtc                                                                                                                <  2:149817/140‑1 (MQ=255)
                                              gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccgg                                                                                                        <  2:347155/148‑3 (MQ=255)
                                              gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccgg                                                                                                        <  5:80961/148‑3 (MQ=255)
                                              gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccgg                                                                                                        <  4:37187/148‑3 (MQ=255)
                                              gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                                       <  1:42187/149‑1 (MQ=255)
                                              gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                                       >  6:103895/1‑149 (MQ=255)
                                                gAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTa                                                                                                     >  5:350840/1‑149 (MQ=255)
                                                 aaCACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtca                                                                                                               <  8:343303/138‑2 (MQ=255)
                                                 aaCACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGAc                                                                                                    >  4:163471/1‑149 (MQ=255)
                                                 aaCACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGGGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccg                                                                                                         <  1:346010/144‑2 (MQ=255)
                                                      ggtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTg                                                                                                >  1:70941/3‑148 (MQ=255)
                                                      ggtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTg                                                                                                >  3:53273/3‑148 (MQ=255)
                                                       gtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGaa                                                                                              <  1:238013/148‑1 (MQ=255)
                                                       gtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGaa                                                                                              <  1:158847/148‑1 (MQ=255)
                                                       cTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGaa                                                                                              <  8:316496/149‑1 (MQ=255)
                                                            cTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGa                                                                                                                                      <  4:316244/104‑1 (MQ=255)
                                                            cTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGaat                                                                                         >  1:91124/1‑147 (MQ=255)
                                                            cTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtt                                                                                         <  6:78789/149‑1 (MQ=255)
                                                            cTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtt                                                                                         >  4:137416/1‑149 (MQ=255)
                                                            cTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtt                                                                                         >  4:252350/1‑149 (MQ=255)
                                                            cTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGGCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtt                                                                                         <  3:356999/149‑1 (MQ=255)
                                                             tCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTATGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAttt                                                                                        >  4:348474/1‑149 (MQ=255)
                                                             tCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAttt                                                                                        >  3:301175/1‑149 (MQ=255)
                                                             tCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAttt                                                                                        >  8:10830/1‑149 (MQ=255)
                                                               agagAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtttt                                                                                       <  5:36756/148‑1 (MQ=255)
                                                               agagAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAttt                                                                                        <  8:208360/147‑1 (MQ=255)
                                                               agagAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTtc                                                                                      >  5:325821/1‑149 (MQ=255)
                                                                 agaaTCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtcac                                                                                                              >  8:280560/3‑121 (MQ=255)
                                                                    aTCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGt                                                                                 <  7:71284/149‑1 (MQ=255)
                                                                     tCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCtgtg                                                                                >  7:127525/1‑149 (MQ=255)
                                                                      ccGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCCCCATCGCACGCCGGGACCATCACCGTGTATGATGATTCACCACCGGGGACGCTGAATGATTTTCTATGTGc                                                                               >  4:225342/1‑149 (MQ=255)
                                                                       cGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGc                                                                               <  1:361737/148‑1 (MQ=255)
                                                                        ggATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCa                                                                             >  2:189355/1‑149 (MQ=255)
                                                                         gATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtca                                                                                                               >  3:22963/1‑113 (MQ=255)
                                                                            gAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAc                                                                         >  4:111733/1‑149 (MQ=255)
                                                                             aaGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCGGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAc                                                                         <  8:258171/148‑1 (MQ=255)
                                                                             aaGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAc                                                                         <  7:352427/148‑1 (MQ=255)
                                                                              aGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACgg                                                                       <  3:174470/149‑1 (MQ=255)
                                                                               gCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACg                                                                        >  3:330246/1‑147 (MQ=255)
                                                                                ccGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGATTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACggag                                                                     >  6:183593/1‑149 (MQ=255)
                                                                                      gTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatgat                                                               <  8:366958/149‑1 (MQ=255)
                                                                                      gTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatgat                                                               <  3:116153/149‑1 (MQ=255)
                                                                                      gTTACAGCATGAATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatgat                                                               <  3:83873/149‑1 (MQ=255)
                                                                                       ttACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGatgatg                                                              <  5:240148/149‑1 (MQ=255)
                                                                                        tACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGc                                                             >  6:136611/1‑149 (MQ=255)
                                                                                         aCAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGcc                                                            >  1:29149/1‑149 (MQ=255)
                                                                                          cAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGccc                                                           <  2:57846/149‑1 (MQ=255)
                                                                                          cAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGccc                                                           <  5:382047/149‑1 (MQ=255)
                                                                                                  atgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAg                                                   <  6:224508/144‑1 (MQ=255)
                                                                                                  atgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAg                                                   <  6:150675/144‑1 (MQ=255)
                                                                                                   tgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAgg                                                  >  7:170587/5‑149 (MQ=255)
                                                                                                    gtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggt                                                 >  7:300622/4‑148 (MQ=255)
                                                                                                      ggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgc                                               >  7:328205/2‑146 (MQ=255)
                                                                                                       gAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgct                                              >  5:151329/1‑145 (MQ=255)
                                                                                                       gAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgct                                              <  4:93357/149‑5 (MQ=255)
                                                                                                       gAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTACTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgct                                              >  7:379866/1‑145 (MQ=255)
                                                                                                       gAGTACGGGCAGTACAGGGTCATCCTGCAGGGTGACGGTTTTCCACCATCGCACGCCGGGACCAGCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgct                                              <  2:116280/149‑5 (MQ=255)
                                                                                                         gTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgc                                            >  6:376915/1‑149 (MQ=255)
                                                                                                         gTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAGTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgc                                            <  7:21/149‑1 (MQ=255)
                                                                                                          tACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcg                                           >  4:64894/1‑149 (MQ=255)
                                                                                                            cGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgt                                          <  6:288394/148‑2 (MQ=255)
                                                                                                            cGGTCAGTACAGTGTCATCCTGCAGGTGGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgtc                                         <  1:22261/149‑3 (MQ=255)
                                                                                                             ggTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatgat                                                               <  6:98682/126‑1 (MQ=255)
                                                                                                             ggTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggt                                                 >  8:69911/1‑139 (MQ=255)
                                                                                                              gTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgt                                       >  7:85075/1‑149 (MQ=255)
                                                                                                              gTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgt                                       >  5:129522/1‑149 (MQ=255)
                                                                                                               tCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtc                                      >  8:39493/1‑148 (MQ=255)
                                                                                                               tCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtc                                      <  1:130280/149‑2 (MQ=255)
                                                                                                               tCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGtt                                      <  6:350840/149‑1 (MQ=255)
                                                                                                                 aGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtct                                     <  7:26755/148‑3 (MQ=255)
                                                                                                                 aGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGtctt                                    <  2:29149/149‑1 (MQ=255)
                                                                                                                   tACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTg                                   >  5:21392/1‑148 (MQ=255)
                                                                                                                             ttCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTCCGATGAATGATTTTCTCTGTGCCATGACGCAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGAtggtg                        >  2:221083/1‑149 (MQ=255)
                                                                                                                               ctgcaggttgACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGGTGCGTCGTCTTGAACTGAtggtgg                       >  5:298370/6‑148 (MQ=255)
                                                                                                                                tgcaggttgACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGAtggtgg                       >  3:205112/5‑147 (MQ=255)
                                                                                                                                tGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGAtggtgg                       >  2:324690/1‑147 (MQ=255)
                                                                                                                                 gcaggttgACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGCtggtgg                       >  5:6153/4‑146 (MQ=255)
                                                                                                                                  caggttgACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTAAGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTTCTGCGTCGTCTTGAACTGATGGTGGaa                     <  1:380308/145‑1 (MQ=255)
                                                                                                                                   aggttgACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAagag                  <  7:45336/148‑1 (MQ=255)
                                                                                                                                             aTTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGt         >  6:331474/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGT  >  NC_000913/1429105‑1429400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 245 total aligned reads displayed.
ACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTC  >  NC_000913/1429104‑1429401
                                                                                                                                                    |                                                                                                                                                     
ACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCC                                                                                                                                                     <  NB501016:230:HFVJLAFXY:4:11402:16572:15101/151‑1 (MQ=60)
     ACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACCCGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCCTGGACGTTGAGTACGGTCAGTACAGCGTTAGTCTGTTGGTGGAAGGATTCCCGCCGTCAC                                                                                                                                                >  NB501016:230:HFVJLAFXY:3:21506:14017:4743/1‑151 (MQ=60)
                GAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACC                                                                                                                                     >  NB501016:230:HFVJLAFXY:1:11102:4631:1745/1‑151 (MQ=60)
                      AAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACC                                                                                                                               >  NB501016:230:HFVJLAFXY:3:21412:18093:12986/1‑151 (MQ=60)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 >  NB501016:230:HFVJLAFXY:2:21110:12583:11778/1‑151 (MQ=53)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 >  NB501016:230:HFVJLAFXY:2:21108:13855:9874/1‑151 (MQ=53)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 >  NB501016:230:HFVJLAFXY:3:11506:17812:3437/1‑151 (MQ=53)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 <  NB501016:230:HFVJLAFXY:2:11208:19672:3163/151‑1 (MQ=53)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 <  NB501016:230:HFVJLAFXY:2:11101:19563:1297/151‑1 (MQ=53)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 >  NB501016:230:HFVJLAFXY:1:21302:22816:7789/1‑151 (MQ=53)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 <  NB501016:230:HFVJLAFXY:1:21112:14510:10122/151‑1 (MQ=53)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11410:16679:4501/1‑151 (MQ=53)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11502:5431:13872/1‑151 (MQ=53)
                                    CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 >  NB501016:230:HFVJLAFXY:1:11210:22870:6771/1‑151 (MQ=53)
                                     agccggtgactggagttcagacgtgctcttccgatctGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 <  NB501016:230:HFVJLAFXY:4:11404:10486:4231/113‑1 (MQ=24)
                                         GTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAC                                                                                                            >  NB501016:230:HFVJLAFXY:1:11101:20729:9816/1‑151 (MQ=45)
                                         GTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAC                                                                                                            >  NB501016:230:HFVJLAFXY:4:21611:12515:11055/1‑151 (MQ=45)
                                         GTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGGACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAAC                                                                                                            <  NB501016:230:HFVJLAFXY:3:21603:20832:17650/151‑1 (MQ=60)
                                                TGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTAC                                                                                                     >  NB501016:230:HFVJLAFXY:3:21607:12389:1312/1‑151 (MQ=60)
                                                  AACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGC                                                                                                   >  NB501016:230:HFVJLAFXY:4:21509:11632:12384/1‑151 (MQ=60)
                                                   acacggggggCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCT                                                                                                  <  NB501016:230:HFVJLAFXY:3:11603:20922:12708/141‑1 (MQ=32)
                                                    CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 <  NB501016:230:HFVJLAFXY:3:11601:12001:1833/151‑1 (MQ=31)
                                                    CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 >  NB501016:230:HFVJLAFXY:4:11503:5857:7462/1‑151 (MQ=31)
                                                    CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 >  NB501016:230:HFVJLAFXY:2:11105:16424:8237/1‑151 (MQ=31)
                                                    CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 <  NB501016:230:HFVJLAFXY:3:11604:13390:10317/151‑1 (MQ=31)
                                                    CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 <  NB501016:230:HFVJLAFXY:2:11309:17978:1286/151‑1 (MQ=31)
                                                    CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 <  NB501016:230:HFVJLAFXY:3:21404:12521:4584/151‑1 (MQ=31)
                                                    CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATCGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 >  NB501016:230:HFVJLAFXY:3:21511:12647:15827/1‑151 (MQ=24)
                                                     ACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 >  NB501016:230:HFVJLAFXY:2:21306:6412:8580/1‑150 (MQ=31)
                                                      CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAA                                                                                               <  NB501016:230:HFVJLAFXY:1:11306:7051:8753/151‑1 (MQ=31)
                                                      CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                                                >  NB501016:230:HFVJLAFXY:4:11406:6936:19814/1‑150 (MQ=31)
                                                      CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                                                >  NB501016:230:HFVJLAFXY:3:21408:14846:11844/1‑150 (MQ=31)
                                                      CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                                                >  NB501016:230:HFVJLAFXY:1:11112:21860:17452/1‑150 (MQ=31)
                                                      CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                                                >  NB501016:230:HFVJLAFXY:2:11110:13058:9521/1‑150 (MQ=31)
                                                      CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                                                >  NB501016:230:HFVJLAFXY:2:11111:12017:12425/1‑150 (MQ=31)
                                                       GGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAT                                                                                              >  NB501016:230:HFVJLAFXY:2:11203:26454:7442/1‑151 (MQ=31)
                                                       GGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAT                                                                                              <  NB501016:230:HFVJLAFXY:3:11408:18858:8969/151‑1 (MQ=31)
                                                       GGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAT                                                                                              <  NB501016:230:HFVJLAFXY:1:21107:7842:7743/151‑1 (MQ=31)
                                                       GGTGGGCTCAGAGAAGCCGGATGAAGCCGGGCGTTTCAGCATGGATGTGGAGTACCGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAT                                                                                              <  NB501016:230:HFVJLAFXY:2:21111:19658:2675/151‑1 (MQ=12)
                                                       GCTTGCCTCAGAAAAACCGGATGAAGCCGGGCGTTACAGCAGGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGAATCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAAT                                                                                              <  NB501016:230:HFVJLAFXY:2:11111:5800:20091/151‑1 (MQ=60)
                                                          GGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGCGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAT                                                                                           <  NB501016:230:HFVJLAFXY:1:21210:25196:3283/151‑1 (MQ=28)
                                                           GGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGCTT                                                                                          >  NB501016:230:HFVJLAFXY:1:11201:7812:14011/1‑151 (MQ=32)
                                                            gcgcagagAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACACCCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:2:11102:21777:15669/143‑1 (MQ=29)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGCCCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         >  NB501016:230:HFVJLAFXY:3:11503:3851:15411/1‑151 (MQ=31)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:2:21302:15753:3456/151‑1 (MQ=38)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:2:21305:17857:12423/151‑1 (MQ=38)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:4:21412:4513:1621/151‑1 (MQ=38)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:4:11604:25337:11985/151‑1 (MQ=38)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:1:21201:20714:5218/151‑1 (MQ=38)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         >  NB501016:230:HFVJLAFXY:4:21408:15996:3489/1‑151 (MQ=38)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:1:21106:16192:10573/151‑1 (MQ=38)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:1:21110:16311:12552/151‑1 (MQ=38)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAATT                                                                                         >  NB501016:230:HFVJLAFXY:1:11204:11202:14282/1‑151 (MQ=33)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCT                                                                                                  >  NB501016:230:HFVJLAFXY:4:11404:16785:3332/1‑142 (MQ=32)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACAGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         >  NB501016:230:HFVJLAFXY:4:11510:24578:7644/1‑151 (MQ=31)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTGGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:1:21306:24356:16318/151‑1 (MQ=45)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGGCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:2:21310:4420:12541/151‑1 (MQ=31)
                                                            GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGGGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         <  NB501016:230:HFVJLAFXY:2:21109:6070:17454/151‑1 (MQ=31)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCCCGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:4:11509:10847:15841/1‑151 (MQ=32)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGCCCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:3:21503:12224:6351/1‑151 (MQ=32)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACCACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:1:21312:5823:15477/1‑151 (MQ=32)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGCATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:2:11211:22424:17248/1‑151 (MQ=32)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:2:21212:23152:5570/1‑151 (MQ=39)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:1:21304:14623:17168/1‑151 (MQ=39)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:4:11409:6429:5801/1‑151 (MQ=39)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:3:11602:17515:15596/1‑151 (MQ=39)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:4:21410:10439:16792/1‑151 (MQ=39)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:2:11305:24691:5255/1‑151 (MQ=39)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:3:21611:22049:18320/1‑151 (MQ=39)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:2:21107:14244:11338/1‑151 (MQ=39)
                                                             CTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACAATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:1:21210:19639:3199/1‑151 (MQ=32)
                                                             CTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTT                                                                                        >  NB501016:230:HFVJLAFXY:1:11102:22950:9733/1‑151 (MQ=60)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACATCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTC                                                                                       <  NB501016:230:HFVJLAFXY:4:21608:1863:2875/150‑1 (MQ=31)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                                      >  NB501016:230:HFVJLAFXY:3:21602:19221:13119/1‑151 (MQ=39)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                                      >  NB501016:230:HFVJLAFXY:4:11509:2055:1584/1‑151 (MQ=39)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                                      <  NB501016:230:HFVJLAFXY:4:11405:24553:14701/151‑1 (MQ=39)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                                      <  NB501016:230:HFVJLAFXY:4:21403:14134:2716/151‑1 (MQ=39)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                                      >  NB501016:230:HFVJLAFXY:3:21609:7248:13522/1‑151 (MQ=39)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                                      >  NB501016:230:HFVJLAFXY:3:11606:10971:4788/1‑151 (MQ=39)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTC                                                                                       <  NB501016:230:HFVJLAFXY:3:11408:18450:3493/150‑1 (MQ=38)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATT                                                                                          <  NB501016:230:HFVJLAFXY:1:11303:4333:12213/147‑1 (MQ=34)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 >  NB501016:230:HFVJLAFXY:1:21302:22075:7635/1‑140 (MQ=29)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTC                                                                                                                 >  NB501016:230:HFVJLAFXY:1:11206:14068:4656/1‑124 (MQ=32)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCACTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                                      >  NB501016:230:HFVJLAFXY:1:21106:3106:2812/1‑151 (MQ=32)
                                                               CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGGGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCGGAATGATTTTCT                                                                                      <  NB501016:230:HFVJLAFXY:3:21506:19567:4789/151‑1 (MQ=25)
                                                                AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCCTCACCGTGTATGAAGATTCACAACCGGGGACGATGAATGATTTTCTC                                                                                     >  NB501016:230:HFVJLAFXY:2:21211:19857:3420/1‑151 (MQ=28)
                                                                AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                                                     <  NB501016:230:HFVJLAFXY:1:11101:23779:13852/151‑1 (MQ=40)
                                                                AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                                                     <  NB501016:230:HFVJLAFXY:4:11501:8778:3576/151‑1 (MQ=40)
                                                                AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                                                     <  NB501016:230:HFVJLAFXY:2:21308:5485:13926/151‑1 (MQ=40)
                                                                AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                                                     <  NB501016:230:HFVJLAFXY:1:21206:25212:10574/151‑1 (MQ=40)
                                                                AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                                                     <  NB501016:230:HFVJLAFXY:2:21202:6358:9062/151‑1 (MQ=40)
                                                                AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGAGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                                                     <  NB501016:230:HFVJLAFXY:4:11510:3897:17042/151‑1 (MQ=40)
                                                                 GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACCGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCCTCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                                                    >  NB501016:230:HFVJLAFXY:1:11203:12483:14166/1‑151 (MQ=27)
                                                                 GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                                                    <  NB501016:230:HFVJLAFXY:4:11502:19749:9583/151‑1 (MQ=40)
                                                                 GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                                                    <  NB501016:230:HFVJLAFXY:1:21110:3841:5639/151‑1 (MQ=40)
                                                                 GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                                                    >  NB501016:230:HFVJLAFXY:4:11405:5953:19439/1‑151 (MQ=40)
                                                                 GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                                                    >  NB501016:230:HFVJLAFXY:1:21106:10788:6363/1‑151 (MQ=40)
                                                                 GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                                                    <  NB501016:230:HFVJLAFXY:3:21612:8714:3294/151‑1 (MQ=40)
                                                                 GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                                                    <  NB501016:230:HFVJLAFXY:2:11311:12299:18596/151‑1 (MQ=40)
                                                                  AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                                                   <  NB501016:230:HFVJLAFXY:2:11209:14698:16651/151‑1 (MQ=40)
                                                                  AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                                                   <  NB501016:230:HFVJLAFXY:3:11501:15592:18779/151‑1 (MQ=40)
                                                                  AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                                                   <  NB501016:230:HFVJLAFXY:1:11310:17825:3078/151‑1 (MQ=40)
                                                                  AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                                                   <  NB501016:230:HFVJLAFXY:1:11109:12504:4349/151‑1 (MQ=40)
                                                                  AGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTACGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTG                                                                                   <  NB501016:230:HFVJLAFXY:4:11401:25033:14153/151‑1 (MQ=33)
                                                                   GAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGT                                                                                  >  NB501016:230:HFVJLAFXY:4:11502:25424:13340/1‑151 (MQ=40)
                                                                   GAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGT                                                                                  <  NB501016:230:HFVJLAFXY:3:21605:2094:2037/151‑1 (MQ=40)
                                                                   GAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGT                                                                                  >  NB501016:230:HFVJLAFXY:1:21307:8876:5050/1‑151 (MQ=40)
                                                                   GAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGAGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGT                                                                                  >  NB501016:230:HFVJLAFXY:4:21612:15589:12701/1‑151 (MQ=33)
                                                                    AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                                                 <  NB501016:230:HFVJLAFXY:4:11412:9805:16078/151‑1 (MQ=40)
                                                                    AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                                                 <  NB501016:230:HFVJLAFXY:1:21104:15407:6162/151‑1 (MQ=40)
                                                                    AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                                                 <  NB501016:230:HFVJLAFXY:4:21605:11456:10464/151‑1 (MQ=40)
                                                                    AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                                                 <  NB501016:230:HFVJLAFXY:3:11605:8603:15290/151‑1 (MQ=40)
                                                                    AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGAACGCTGAATGATTTTCTCTGTG                                                                                 <  NB501016:230:HFVJLAFXY:2:21207:19975:1916/151‑1 (MQ=40)
                                                                    AATCCGGATGAAGCCGGGCGTTACAGCATGGAGGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                                                 <  NB501016:230:HFVJLAFXY:1:11308:22444:14936/151‑1 (MQ=40)
                                                                     ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCCCCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                                                >  NB501016:230:HFVJLAFXY:3:11408:14627:4136/1‑151 (MQ=39)
                                                                     ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                                                >  NB501016:230:HFVJLAFXY:3:11409:19488:17341/1‑151 (MQ=39)
                                                                     ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                                                >  NB501016:230:HFVJLAFXY:4:11510:4201:17245/1‑151 (MQ=39)
                                                                     ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                 >  NB501016:230:HFVJLAFXY:4:21402:20093:3370/1‑134 (MQ=29)
                                                                      cgCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                                               <  NB501016:230:HFVJLAFXY:2:11202:4921:17862/149‑1 (MQ=36)
                                                                      TCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                                               >  NB501016:230:HFVJLAFXY:2:11202:19615:18118/1‑151 (MQ=39)
                                                                      TCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                                                <  NB501016:230:HFVJLAFXY:1:21101:9462:5158/150‑1 (MQ=38)
                                                                      TCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                         >  NB501016:230:HFVJLAFXY:4:21408:14946:19257/1‑141 (MQ=33)
                                                                       CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATTGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                                               <  NB501016:230:HFVJLAFXY:1:11211:18733:15713/150‑1 (MQ=31)
                                                                       CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCA                                                                              <  NB501016:230:HFVJLAFXY:1:21304:21745:16764/151‑1 (MQ=39)
                                                                       CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCA                                                                              <  NB501016:230:HFVJLAFXY:1:11303:22311:14617/151‑1 (MQ=39)
                                                                       CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                                               >  NB501016:230:HFVJLAFXY:2:21210:22033:13801/1‑150 (MQ=38)
                                                                        CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCCCCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                             >  NB501016:230:HFVJLAFXY:4:11401:19556:15659/1‑151 (MQ=39)
                                                                        CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                             <  NB501016:230:HFVJLAFXY:4:21405:7081:11791/151‑1 (MQ=39)
                                                                        CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                             <  NB501016:230:HFVJLAFXY:1:11304:10905:4771/151‑1 (MQ=39)
                                                                        CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                             >  NB501016:230:HFVJLAFXY:1:21101:4575:9154/1‑151 (MQ=39)
                                                                        CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                             >  NB501016:230:HFVJLAFXY:4:21508:17114:10684/1‑151 (MQ=39)
                                                                        CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                             <  NB501016:230:HFVJLAFXY:2:11206:16898:7762/151‑1 (MQ=39)
                                                                        CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGGTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                             >  NB501016:230:HFVJLAFXY:1:11212:4279:8408/1‑151 (MQ=32)
                                                                         GGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATG                                                                            <  NB501016:230:HFVJLAFXY:4:11608:17299:2208/151‑1 (MQ=39)
                                                                         GGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATG                                                                            >  NB501016:230:HFVJLAFXY:1:21303:11422:13560/1‑151 (MQ=39)
                                                                         GGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCGCTGTGCCATG                                                                            <  NB501016:230:HFVJLAFXY:3:11503:7394:14353/151‑1 (MQ=32)
                                                                         GGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCGGAATGATTTTCTCTGTGCCATG                                                                            <  NB501016:230:HFVJLAFXY:3:21405:17068:5740/151‑1 (MQ=32)
                                                                         GGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACAATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                                               >  NB501016:230:HFVJLAFXY:1:11111:9474:7580/1‑148 (MQ=28)
                                                                           ATGAAGCCGGGCGTTACAGCATGGATGTTGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGGTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAC                                                                          <  NB501016:230:HFVJLAFXY:3:11607:24699:16725/151‑1 (MQ=47)
                                                                           ATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAC                                                                          <  NB501016:230:HFVJLAFXY:3:11611:12030:10451/151‑1 (MQ=39)
                                                                           ATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGAACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAC                                                                          >  NB501016:230:HFVJLAFXY:3:21512:5977:15482/1‑151 (MQ=32)
                                                                            TGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACG                                                                         >  NB501016:230:HFVJLAFXY:3:11602:24328:15634/1‑151 (MQ=39)
                                                                             GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        <  NB501016:230:HFVJLAFXY:3:21503:15774:5234/151‑1 (MQ=39)
                                                                             GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:2:21303:22721:15364/1‑151 (MQ=39)
                                                                             GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        <  NB501016:230:HFVJLAFXY:3:21603:11297:6861/151‑1 (MQ=39)
                                                                             GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACG                                                                         <  NB501016:230:HFVJLAFXY:1:21105:14575:5696/150‑1 (MQ=38)
                                                                             GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACG                                                                         <  NB501016:230:HFVJLAFXY:4:21604:1795:4662/150‑1 (MQ=38)
                                                                             GAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:2:11108:17346:14621/1‑151 (MQ=60)
                                                                              AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGCCCATCACCGTGTATGAAGATTCACAACCGGGGACGCGGAATGATTTTCTCTGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:3:21609:2612:4191/1‑150 (MQ=24)
                                                                              AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGA                                                                       <  NB501016:230:HFVJLAFXY:2:11310:13964:17232/151‑1 (MQ=39)
                                                                              AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:4:11405:2316:10093/1‑150 (MQ=38)
                                                                              AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:4:21608:1259:15009/1‑150 (MQ=38)
                                                                              AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:1:11211:10706:8602/1‑150 (MQ=38)
                                                                              AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCT                                                                                      >  NB501016:230:HFVJLAFXY:3:11406:15399:15539/1‑136 (MQ=27)
                                                                              AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATT                                                                                          >  NB501016:230:HFVJLAFXY:1:21310:10093:16374/1‑132 (MQ=22)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                                      <  NB501016:230:HFVJLAFXY:4:21409:20939:12187/151‑1 (MQ=39)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                                      <  NB501016:230:HFVJLAFXY:2:11112:10751:14771/151‑1 (MQ=39)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                                      <  NB501016:230:HFVJLAFXY:1:21106:9036:17548/151‑1 (MQ=39)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                                      <  NB501016:230:HFVJLAFXY:2:11303:18635:2469/151‑1 (MQ=39)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                                      <  NB501016:230:HFVJLAFXY:4:21411:22917:11511/151‑1 (MQ=39)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:1:11203:20262:16968/1‑149 (MQ=36)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:3:21406:5453:19576/1‑149 (MQ=36)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:2:21305:4650:15597/1‑149 (MQ=36)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:3:11610:18230:2428/1‑149 (MQ=36)
                                                                               AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGA                                                                                            >  NB501016:230:HFVJLAFXY:2:21110:5432:8238/1‑129 (MQ=18)
                                                                               AGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCAGAATGATTTTCTCGGTGCCATGACGG                                                                        >  NB501016:230:HFVJLAFXY:4:11411:23191:2175/1‑149 (MQ=60)
                                                                                GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCTCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                                                     <  NB501016:230:HFVJLAFXY:1:11102:10709:19629/151‑1 (MQ=39)
                                                                                GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                                                     >  NB501016:230:HFVJLAFXY:2:11105:23914:12718/1‑151 (MQ=39)
                                                                                GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                                                     <  NB501016:230:HFVJLAFXY:3:11606:21480:18540/151‑1 (MQ=39)
                                                                                GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                                                     <  NB501016:230:HFVJLAFXY:2:21305:12106:6212/151‑1 (MQ=39)
                                                                                GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                                                     <  NB501016:230:HFVJLAFXY:4:21607:22325:5971/151‑1 (MQ=39)
                                                                                GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                                                     <  NB501016:230:HFVJLAFXY:3:11408:10988:19980/151‑1 (MQ=39)
                                                                                 CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAATATTCACAACCCGGGACGCAGAATGATTTTCTCTGTGCCATGACGCAGG                                                                     >  NB501016:230:HFVJLAFXY:4:11606:21059:14280/1‑150 (MQ=25)
                                                                                 CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGA                                                                    <  NB501016:230:HFVJLAFXY:4:21601:13960:13705/151‑1 (MQ=39)
                                                                                 CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGA                                                                    <  NB501016:230:HFVJLAFXY:4:11602:24983:4926/151‑1 (MQ=39)
                                                                                 CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGA                                                                    <  NB501016:230:HFVJLAFXY:4:11406:25639:10528/151‑1 (MQ=39)
                                                                                 CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                                                     >  NB501016:230:HFVJLAFXY:4:21609:24135:5552/1‑150 (MQ=38)
                                                                                 CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGAC                                                                          <  NB501016:230:HFVJLAFXY:4:11511:23497:13160/145‑1 (MQ=31)
                                                                                 CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATAACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGA                                                                    <  NB501016:230:HFVJLAFXY:3:21402:23339:17791/151‑1 (MQ=39)
                                                                                     GCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGAT                                                                <  NB501016:230:HFVJLAFXY:4:11607:10868:9091/151‑1 (MQ=42)
                                                                                     GCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGAT                                                                <  NB501016:230:HFVJLAFXY:1:11203:17781:9119/151‑1 (MQ=42)
                                                                                      CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                               <  NB501016:230:HFVJLAFXY:2:11212:24818:5863/151‑1 (MQ=43)
                                                                                      CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                               <  NB501016:230:HFVJLAFXY:1:11107:19305:13527/151‑1 (MQ=43)
                                                                                      CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                               >  NB501016:230:HFVJLAFXY:4:11404:15501:9305/1‑151 (MQ=43)
                                                                                      CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                               <  NB501016:230:HFVJLAFXY:2:11308:25001:19209/151‑1 (MQ=43)
                                                                                      CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                               <  NB501016:230:HFVJLAFXY:1:21304:8285:2360/151‑1 (MQ=43)
                                                                                      CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                               >  NB501016:230:HFVJLAFXY:1:11307:9102:11192/1‑151 (MQ=43)
                                                                                       GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGC                                                              <  NB501016:230:HFVJLAFXY:1:11303:18955:17168/151‑1 (MQ=45)
                                                                                       GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGC                                                              <  NB501016:230:HFVJLAFXY:2:11205:20778:10546/151‑1 (MQ=45)
                                                                                       GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGC                                                              <  NB501016:230:HFVJLAFXY:4:11412:15902:15536/151‑1 (MQ=45)
                                                                                       GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGC                                                              <  NB501016:230:HFVJLAFXY:4:21511:20423:5758/151‑1 (MQ=45)
                                                                                       GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGC                                                              <  NB501016:230:HFVJLAFXY:3:21409:5560:12232/151‑1 (MQ=45)
                                                                                       GTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGAGGC                                                              >  NB501016:230:HFVJLAFXY:3:21609:8619:9568/1‑151 (MQ=40)
                                                                                          ACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCG                                                           <  NB501016:230:HFVJLAFXY:3:21612:14014:10109/151‑1 (MQ=60)
                                                                                              CATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGAT                                                                   >  NB501016:230:HFVJLAFXY:3:21607:25995:14169/1‑139 (MQ=60)
                                                                                                    CGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTC                                                                                       <  NB501016:230:HFVJLAFXY:2:21112:17941:11842/113‑1 (MQ=60)
                                                                                                             CGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACG                                                                         >  NB501016:230:HFVJLAFXY:3:21606:14987:5447/1‑118 (MQ=60)
                                                                                                                 CAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTG                                    >  NB501016:230:HFVJLAFXY:3:21612:23237:19978/1‑151 (MQ=60)
                                                                                                                                        GGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCG             >  NB501016:230:HFVJLAFXY:4:11506:4493:3908/1‑151 (MQ=60)
                                                                                                                                                   CGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTC  >  NB501016:230:HFVJLAFXY:4:21510:7157:1609/1‑151 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTC  >  NC_000913/1429104‑1429401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑