Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,429,291 T→A 100% S81S (TCT→TCA)  stfR → Rac prophage; putative tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,429,2910TA96.3% 4539.0 / 89.6 1600S81S (TCT→TCA) stfRRac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base T (32/30);  new base A (781/751);  total (817/783)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 1604 total aligned reads displayed.

TGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAG  >  NC_000913/1429146‑1429439
                                                                                                                                                 |                                                                                                                                                    
     gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                                                                              <  7:249310/149‑1 (MQ=255)
     gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                                                                              <  2:211325/149‑1 (MQ=255)
     gtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                                                                              <  6:380980/149‑1 (MQ=255)
       gAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccg                                                                                                                                                >  3:223607/1‑144 (MQ=255)
       gAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCgggga                                                                                                                                            >  3:171384/1‑147 (MQ=255)
       gAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATCTGGAGTACGGGCAGTACAGTGTCTTCCTGCAGGTTGCCGGTTTTCCACCATCGCACGCCGGGACCATCCCCGTGTATGAAGATTCACAAccgcgga                                                                                                                                            >  1:327275/1‑144 (MQ=255)
         acacGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                                                                              <  4:175442/145‑1 (MQ=255)
             ggtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTg                                                                                                                                       >  3:53273/3‑148 (MQ=255)
              gtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCTTGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTGTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGaa                                                                                                                                     <  4:202010/148‑1 (MQ=255)
              gtGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGaa                                                                                                                                     <  5:38256/148‑1 (MQ=255)
              gggggcTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTCTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGaa                                                                                                                                     <  7:327163/144‑1 (MQ=255)
              cTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGaa                                                                                                                                     <  8:316496/149‑1 (MQ=255)
               tGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAt                                                                                                                                    <  3:7993/149‑1 (MQ=255)
                   cTCAGAGAATCCGGATGAAGCCGGGCGTTATAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtt                                                                                                                                >  8:26139/1‑149 (MQ=255)
                   cTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtt                                                                                                                                >  2:148174/1‑149 (MQ=255)
                    tCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAttt                                                                                                                               >  4:311789/1‑149 (MQ=255)
                    tCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAttt                                                                                                                               >  4:229397/1‑149 (MQ=255)
                     cAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtttt                                                                                                                              >  6:212803/1‑149 (MQ=255)
                      agagAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtttt                                                                                                                              <  5:36756/148‑1 (MQ=255)
                      agagAATCCGGATGAAGCCGGGCGTTACAGCATGGAAGTGGAGTACGGGCAGTACAGTGGCATCCTGCAGGTTGACGGTTTTCCTCCATCGCACGCCGGGCCGATCACCGTGTATGCAGCTTCTCAACGGGGGACGCTGAATGATTTtc                                                                                                                             >  6:299344/1‑149 (MQ=255)
                       gagaaTCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTtct                                                                                                                            <  1:4171/146‑1 (MQ=255)
                           aTCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGt                                                                                                                        <  1:192581/149‑1 (MQ=255)
                           aTCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGt                                                                                                                        <  6:360755/149‑1 (MQ=255)
                               ggATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCt                                                                                                                                        <  3:109116/129‑1 (MQ=255)
                               ggATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCa                                                                                                                    >  7:310659/1‑149 (MQ=255)
                               ggATGAAGCCGGGCGTTACAGCAGGGATGTGGAGTACGCTCACTACAGTGTCATCCTGCAGGTGGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCa                                                                                                                    <  2:348660/149‑1 (MQ=255)
                                   gAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                                                                              <  1:37095/119‑1 (MQ=255)
                                    aaGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGACGGGACCATCACCGTGTATGAAGATTCACAACCggg                                                                                                                                              >  4:149452/1‑118 (MQ=255)
                                     aGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCCCAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACg                                                                                                               >  2:370767/1‑148 (MQ=255)
                                      gCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACg                                                                                                               >  5:226409/1‑147 (MQ=255)
                                             gTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatgat                                                                                                      <  1:349010/149‑1 (MQ=255)
                                                   gCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCgg                                                                                                <  7:47282/149‑2 (MQ=255)
                                                    cATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGTCCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatgat                                                                                                      >  1:218986/1‑142 (MQ=255)
                                                    cATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCggc                                                                                               >  1:7095/1‑147 (MQ=255)
                                                         atgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAg                                                                                          <  5:146115/144‑1 (MQ=255)
                                                         aggtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCCTCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAg                                                                                          <  5:264048/144‑1 (MQ=255)
                                                          tgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatgat                                                                                                      >  2:315752/5‑136 (MQ=255)
                                                              gAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGatgatg                                                                                                     <  7:281534/133‑1 (MQ=255)
                                                               aGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgctg                                                                                    >  4:128334/1‑144 (MQ=255)
                                                                gTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgc                                                                                   <  6:83105/149‑1 (MQ=255)
                                                                gTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgc                                                                                   <  1:269689/149‑1 (MQ=255)
                                                                gTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgc                                                                                   >  5:288394/1‑149 (MQ=255)
                                                                 tACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcg                                                                                  >  4:64894/1‑149 (MQ=255)
                                                                   cGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCCCGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgtc                                                                                >  6:60979/1‑147 (MQ=255)
                                                                   cGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgtc                                                                                <  4:368038/149‑3 (MQ=255)
                                                                   cGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgtc                                                                                <  3:248373/149‑3 (MQ=255)
                                                                   cGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgtc                                                                                >  6:118031/1‑147 (MQ=255)
                                                                    ggTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggt                                                                                        >  8:69911/1‑139 (MQ=255)
                                                                    ggTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCg                                                                               >  6:252353/1‑149 (MQ=255)
                                                                    ggTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCg                                                                               >  8:174208/1‑149 (MQ=255)
                                                                    ggTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGAc                                                                                                                >  5:346316/1‑116 (MQ=255)
                                                                      tCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtc                                                                             >  7:119513/1‑148 (MQ=255)
                                                                      tCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtc                                                                             <  7:7933/149‑2 (MQ=255)
                                                                      tCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtc                                                                             <  8:10758/149‑2 (MQ=255)
                                                                        aGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGtctt                                                                           >  1:90326/1‑149 (MQ=255)
                                                                          tACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgct                                                                                     <  7:385870/137‑5 (MQ=255)
                                                                          tACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgtc                                                                                >  5:314360/1‑140 (MQ=255)
                                                                           aCAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGTTGCGTCGTCTTGaa                                                                        >  5:64698/1‑149 (MQ=255)
                                                                                        gcaggttgACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGAtggtgg                                                              >  8:244098/4‑146 (MQ=255)
                                                                                          aggttgACGGTTTTCCACCATCGCACGCCGGTACCATCACCGTGTATGAAGATTAACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAagag                                                         <  4:275822/148‑1 (MQ=255)
                                                                                          aggttgACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAagag                                                         <  7:155867/148‑1 (MQ=255)
                                                                                           ggttgACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAgg                                                        <  3:366085/149‑1 (MQ=255)
                                                                                                              tcgcacgCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTaa                                              <  1:129486/134‑1 (MQ=255)
                                                                                                              tcgcacgCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCcgt                                     <  3:189731/143‑2 (MQ=255)
                                                                                                                gcacgCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCcgtg                                    <  1:235383/144‑3 (MQ=255)
                                                                                                                gcacgCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCcgtg                                    <  8:396231/144‑3 (MQ=255)
                                                                                                                gcacgCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTgg                                   <  8:144401/145‑1 (MQ=255)
                                                                                                                acaTGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCgg                                   <  8:350588/149‑1 (MQ=255)
                                                                                                                  acgCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCgtggtg                                 <  3:310165/147‑1 (MQ=255)
                                                                                                                  acgCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCgtggtg                                 <  8:372478/147‑1 (MQ=255)
                                                                                                                   cgCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGtggtgg                                <  1:307225/148‑1 (MQ=255)
                                                                                                                      cGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTGCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGcac                             <  3:149363/149‑1 (MQ=255)
                                                                                                                        ggACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGca                              >  7:218152/1‑146 (MQ=255)
                                                                                                                         gagcATGACCGTGTATGAACTTTCACTACCGGGGACGCAGAATGATTTTCGCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGGCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGGCCGTGGTGGCACAGa                          <  4:92910/146‑1 (MQ=255)
                                                                                                                           ccATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTTGTGGCACAGagt                        <  4:253480/149‑3 (MQ=255)
                                                                                                                            cATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGTCGCGTAACGCGTCCGTGGTGGCACCGagta                       >  2:286973/1‑146 (MQ=255)
                                                                                                                            cATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGagta                       >  5:19852/1‑146 (MQ=255)
                                                                                                                            cATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGagta                       <  8:296597/149‑4 (MQ=255)
                                                                                                                              tcaCCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGTAGGTGCTGCGTAGTCTTGATCTGATGGTGGAAGACGTGGCGCGTAACGCGTCCGTGGGGGCAgagagttag                     >  2:391926/3‑144 (MQ=255)
                                                                                                                              tcaCCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGagtacg                     >  3:330407/3‑144 (MQ=255)
                                                                                                                                aCCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACgg                    <  2:234872/148‑1 (MQ=255)
                                                                                                                                aCCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACgg                    <  6:330468/148‑1 (MQ=255)
                                                                                                                                aCCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGc                   <  5:16523/149‑1 (MQ=255)
                                                                                                                                aCCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGATGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACgg                    <  2:112437/148‑1 (MQ=255)
                                                                                                                                  cGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGtctt                                                                           >  3:27688/1‑91 (MQ=255)
                                                                                                                                   gtgtATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTgcgtc                                                                                >  5:113160/1‑83 (MQ=255)
                                                                                                                                    tgtATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGAGGCACAGAGTACGGCAgac               >  2:331601/1‑147 (MQ=255)
                                                                                                                                       aTGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCCCAGAGTACGGCAGAcgc             >  6:177599/1‑148 (MQ=255)
                                                                                                                                        tGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAgg                                                        <  4:103139/104‑1 (MQ=255)
                                                                                                                                        tGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGAcgcg            >  2:79962/1‑148 (MQ=255)
                                                                                                                                        tGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGAcgcg            >  4:337518/1‑148 (MQ=255)
                                                                                                                                         gatgATTCACAACCGGGGACGCTGATTGATTTTCTCTGTGACTTGACGGAGTATGATTCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGTCAgac               <  2:183955/141‑3 (MQ=255)
                                                                                                                                         gAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGcgtcgtc                                                                             >  5:82687/1‑81 (MQ=255)
                                                                                                                                         gAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACt                                                                      >  2:62058/1‑89 (MQ=255)
                                                                                                                                          aaGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGAcgcg            >  1:160572/1‑146 (MQ=255)
                                                                                                                                          aaGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCgaa          <  1:100743/148‑1 (MQ=255)
                                                                                                                                           aGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTTGATGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGTCAGACGCgaag         >  8:205572/1‑148 (MQ=255)
                                                                                                                                           aGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCgaag         >  6:147584/1‑148 (MQ=255)
                                                                                                                                            gATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCgtggtg                                 <  7:194340/123‑1 (MQ=255)
                                                                                                                                             aTTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCgaagaaa      >  7:357564/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAG  >  NC_000913/1429146‑1429439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 1058 total aligned reads displayed.
GTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGC  >  NC_000913/1429145‑1429440
                                                                                                                                                  |                                                                                                                                                     
GGGGTGGTGAAGACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTAGAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAC                                                                                                                                                   >  NB501016:230:HFVJLAFXY:2:11310:3975:20374/1‑151 (MQ=28)
  GGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGGGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACC                                                                                                                                                  <  NB501016:230:HFVJLAFXY:2:11207:19876:7467/150‑1 (MQ=35)
       TGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTAC                                                                                                                                            >  NB501016:230:HFVJLAFXY:3:21607:12389:1312/1‑151 (MQ=60)
           CACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTG                                                                                                                                        <  NB501016:230:HFVJLAFXY:3:11604:13390:10317/151‑1 (MQ=31)
             CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAA                                                                                                                                      <  NB501016:230:HFVJLAFXY:1:11306:7051:8753/151‑1 (MQ=31)
             CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                                                                                       >  NB501016:230:HFVJLAFXY:2:11110:13058:9521/1‑150 (MQ=31)
             CGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGA                                                                                                                                       >  NB501016:230:HFVJLAFXY:2:11111:12017:12425/1‑150 (MQ=31)
               gtgggCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATG                                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11102:21029:11704/146‑1 (MQ=33)
                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                                                                <  NB501016:230:HFVJLAFXY:1:21201:20714:5218/151‑1 (MQ=38)
                   GCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTGGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                                                                <  NB501016:230:HFVJLAFXY:1:21306:24356:16318/151‑1 (MQ=45)
                      CAGAGAATCCGGATGAAGCCGGGCGTTACATCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTC                                                                                                                              <  NB501016:230:HFVJLAFXY:4:21608:1863:2875/150‑1 (MQ=31)
                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTC                                                                                                                              <  NB501016:230:HFVJLAFXY:3:21503:10047:18090/150‑1 (MQ=38)
                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATT                                                                                                                                 <  NB501016:230:HFVJLAFXY:1:11303:4333:12213/147‑1 (MQ=34)
                      CAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGGGTCATCCTGCAGTTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTT                                                                                                                                <  NB501016:230:HFVJLAFXY:3:21604:12167:19960/148‑1 (MQ=28)
                       AGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTC                                                                                                                            <  NB501016:230:HFVJLAFXY:2:21308:5485:13926/151‑1 (MQ=40)
                        GAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT                                                                                                                           <  NB501016:230:HFVJLAFXY:3:21612:8714:3294/151‑1 (MQ=40)
                          GAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGT                                                                                                                         <  NB501016:230:HFVJLAFXY:3:21605:2094:2037/151‑1 (MQ=40)
                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                                                                                        <  NB501016:230:HFVJLAFXY:4:11412:9805:16078/151‑1 (MQ=40)
                           AATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGGCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTG                                                                                                                        <  NB501016:230:HFVJLAFXY:1:11106:11577:14295/151‑1 (MQ=33)
                            ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                                                                                       >  NB501016:230:HFVJLAFXY:4:11510:4201:17245/1‑151 (MQ=39)
                            ATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                                                                                       >  NB501016:230:HFVJLAFXY:3:11409:19488:17341/1‑151 (MQ=39)
                             TCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGC                                                                                                                       <  NB501016:230:HFVJLAFXY:1:21101:9462:5158/150‑1 (MQ=38)
                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATTGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                                                                                      <  NB501016:230:HFVJLAFXY:1:11211:18733:15713/150‑1 (MQ=31)
                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCA                                                                                                                     <  NB501016:230:HFVJLAFXY:1:21304:21745:16764/151‑1 (MQ=39)
                              CCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCC                                                                                                                      >  NB501016:230:HFVJLAFXY:2:21210:22033:13801/1‑150 (MQ=38)
                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                                                                    >  NB501016:230:HFVJLAFXY:4:11402:13202:13745/1‑151 (MQ=39)
                               CGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGACGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                                                                    >  NB501016:230:HFVJLAFXY:2:21306:5827:11176/1‑151 (MQ=32)
                                    GAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                                                               >  NB501016:230:HFVJLAFXY:2:21303:22721:15364/1‑151 (MQ=39)
                                     AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGCCCATCACCGTGTATGAAGATTCACAACCGGGGACGCGGAATGATTTTCTCTGTGCCATGACGG                                                                                                               >  NB501016:230:HFVJLAFXY:3:21609:2612:4191/1‑150 (MQ=24)
                                     AAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGG                                                                                                               >  NB501016:230:HFVJLAFXY:4:11405:2316:10093/1‑150 (MQ=38)
                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                                                                             <  NB501016:230:HFVJLAFXY:2:11112:10751:14771/151‑1 (MQ=39)
                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAG                                                                                                             <  NB501016:230:HFVJLAFXY:1:21106:9036:17548/151‑1 (MQ=39)
                                      AGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAT                                                                                                                    >  NB501016:230:HFVJLAFXY:4:21506:16759:18615/1‑144 (MQ=30)
                                       GCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGG                                                                                                            <  NB501016:230:HFVJLAFXY:3:11408:10988:19980/151‑1 (MQ=39)
                                        CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGA                                                                                                           <  NB501016:230:HFVJLAFXY:4:11602:24983:4926/151‑1 (MQ=39)
                                        CCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGA                                                                                                           <  NB501016:230:HFVJLAFXY:4:11406:25639:10528/151‑1 (MQ=39)
                                             CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                                                                      <  NB501016:230:HFVJLAFXY:2:11212:24818:5863/151‑1 (MQ=43)
                                             CGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                                                                      <  NB501016:230:HFVJLAFXY:1:11107:19305:13527/151‑1 (MQ=43)
                                                tacagcatggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCC                                                                                                    <  NB501016:230:HFVJLAFXY:1:21111:16930:3728/81‑1 (MQ=60)
                                                 acagcatggatgtggagtacgggcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCC                                                                                                   <  NB501016:230:HFVJLAFXY:4:11502:17930:11994/82‑1 (MQ=60)
                                                  cagcatggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGG                                                                                                 <  NB501016:230:HFVJLAFXY:1:11101:21655:15193/84‑1 (MQ=60)
                                                   agcatggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGC                                                                                                >  NB501016:230:HFVJLAFXY:4:11604:4852:2498/67‑151 (MQ=60)
                                                    gcatggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGTCCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                                                                      >  NB501016:230:HFVJLAFXY:1:21104:17121:3365/66‑144 (MQ=60)
                                                    gcatggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCC                                                                                               >  NB501016:230:HFVJLAFXY:4:21402:26206:6910/66‑151 (MQ=60)
                                                     catggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCG                                                                                              >  NB501016:230:HFVJLAFXY:4:11403:15608:16030/65‑151 (MQ=60)
                                                     catggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCG                                                                                              >  NB501016:230:HFVJLAFXY:4:21609:22357:19815/65‑151 (MQ=60)
                                                      atggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGG                                                                                             <  NB501016:230:HFVJLAFXY:3:21608:10116:5676/88‑1 (MQ=60)
                                                        ggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAG                                                                                           <  NB501016:230:HFVJLAFXY:4:11408:15855:5883/90‑1 (MQ=60)
                                                         gatggggagtacggtcagtacagtgtcatcctgcaggttgacggtgttccaccatcgcacGCCGGGACCCTCACCGTGTATGAAGCTTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGG                                                                                          <  NB501016:230:HFVJLAFXY:2:11304:19977:11621/91‑1 (MQ=59)
                                                          atgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGT                                                                                         >  NB501016:230:HFVJLAFXY:4:11606:15532:11697/60‑151 (MQ=60)
                                                          atgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATG                                                                                                      <  NB501016:230:HFVJLAFXY:1:21209:7686:15434/79‑1 (MQ=60)
                                                            ggggaggacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGCTTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgc                                                                                       <  NB501016:230:HFVJLAFXY:3:11406:26081:18835/94‑4 (MQ=60)
                                                              ggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACTCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctg                                                                                     >  NB501016:230:HFVJLAFXY:1:11207:24577:19764/56‑146 (MQ=60)
                                                               gagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGC                                                                                    >  NB501016:230:HFVJLAFXY:3:21507:18909:5183/55‑151 (MQ=60)
                                                                cgtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCG                                                                                   <  NB501016:230:HFVJLAFXY:4:21512:20810:7535/98‑1 (MQ=60)
                                                                cgtacgggcagtacagtggcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCG                                                                                   <  NB501016:230:HFVJLAFXY:1:11209:8318:13476/98‑1 (MQ=60)
                                                                agtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCG                                                                                   >  NB501016:230:HFVJLAFXY:4:11506:10265:15479/54‑151 (MQ=60)
                                                                agtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCG                                                                                   <  NB501016:230:HFVJLAFXY:4:21605:11890:14179/98‑1 (MQ=60)
                                                                agtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATAAACGTGTATGAAGATTCACAACCGTGGACGCTTAATGATTTTCTCTGTGCCATGACGGAGGATGATTCCCGGCCGGAGGtgatgcg                                                                                   >  NB501016:230:HFVJLAFXY:2:21112:18944:9074/54‑144 (MQ=37)
                                                                 gtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgcgt                                                                                  >  NB501016:230:HFVJLAFXY:1:21310:18071:5296/53‑143 (MQ=60)
                                                                   acggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgcggcg                                                                                <  NB501016:230:HFVJLAFXY:3:11606:10922:1290/101‑11 (MQ=60)
                                                                    cggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGT                                                                               >  NB501016:230:HFVJLAFXY:3:21610:23748:10192/50‑151 (MQ=60)
                                                                        cagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCCCCACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgcgtcgtcttg                                                                           >  NB501016:230:HFVJLAFXY:2:11209:18957:10424/46‑136 (MQ=60)
                                                                        cagtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgcgtcgtcttg                                                                           >  NB501016:230:HFVJLAFXY:1:11204:25408:11348/46‑136 (MQ=60)
                                                                        cagtacagtggcatcctgcagggtgacggttttccaccatcgcacGCCGGGACCAGCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgcgtcgtcttg                                                                           <  NB501016:230:HFVJLAFXY:1:21312:16241:13059/106‑16 (MQ=60)
                                                                          gtacagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGA                                                                          >  NB501016:230:HFVJLAFXY:4:11504:13809:4575/44‑150 (MQ=60)
                                                                            acagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACT                                                                       >  NB501016:230:HFVJLAFXY:2:21101:4622:3341/42‑151 (MQ=60)
                                                                            acagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACT                                                                       <  NB501016:230:HFVJLAFXY:4:21407:25621:16008/110‑1 (MQ=60)
                                                                            acagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACT                                                                       <  NB501016:230:HFVJLAFXY:1:11305:21557:15114/110‑1 (MQ=60)
                                                                             cagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGACTGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgcggcgtcgtcttgaactg                                                                      <  NB501016:230:HFVJLAFXY:1:21301:8014:15713/111‑21 (MQ=60)
                                                                             cagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTG                                                                      >  NB501016:230:HFVJLAFXY:2:21109:1836:8696/41‑151 (MQ=60)
                                                                             cagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTG                                                                      >  NB501016:230:HFVJLAFXY:4:11608:7316:20347/41‑151 (MQ=60)
                                                                             cagtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTG                                                                      <  NB501016:230:HFVJLAFXY:3:11507:10429:11870/111‑1 (MQ=60)
                                                                              agtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGCCGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTG                                                                      >  NB501016:230:HFVJLAFXY:3:11501:2499:14213/40‑150 (MQ=60)
                                                                              agtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGA                                                                     <  NB501016:230:HFVJLAFXY:1:21303:17540:2617/112‑1 (MQ=60)
                                                                               gtgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGAT                                                                    >  NB501016:230:HFVJLAFXY:4:11409:6755:6599/39‑151 (MQ=60)
                                                                                tgtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTTTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACGGATG                                                                   >  NB501016:230:HFVJLAFXY:3:21504:17294:6963/38‑151 (MQ=58)
                                                                                 gtcatcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGGCGTCTTGAACTGATGG                                                                  <  NB501016:230:HFVJLAFXY:2:21202:9159:7008/115‑1 (MQ=60)
                                                                                   catcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTG                                                                >  NB501016:230:HFVJLAFXY:4:21508:13951:11610/35‑151 (MQ=60)
                                                                                    atcctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGG                                                               <  NB501016:230:HFVJLAFXY:1:11212:9422:1833/118‑1 (MQ=60)
                                                                                    ATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGG                                                               >  NB501016:230:HFVJLAFXY:4:21603:3361:17305/1‑151 (MQ=60)
                                                                                      cctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGGTGCGTCGTCTTGAACTGATGGTGGA                                                              >  NB501016:230:HFVJLAFXY:3:21509:7870:12551/32‑150 (MQ=60)
                                                                                      cctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGA                                                              <  NB501016:230:HFVJLAFXY:2:11202:6045:6244/119‑1 (MQ=60)
                                                                                       ctgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGATCTGTTGGTGGA                                                              >  NB501016:230:HFVJLAFXY:1:11208:26214:2699/31‑149 (MQ=60)
                                                                                        tgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGA                                                              >  NB501016:230:HFVJLAFXY:2:11309:15050:18080/30‑148 (MQ=60)
                                                                                        tgcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGA                                                              >  NB501016:230:HFVJLAFXY:3:21405:5290:7417/30‑148 (MQ=60)
                                                                                         gcaggttgacggttttccaccatcgcacGCCGGTACCATCACCGTGTAAGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTTCTGCGTCGTCTTGAACTGATGGTGGAAG                                                            <  NB501016:230:HFVJLAFXY:1:21309:1246:17473/121‑1 (MQ=60)
                                                                                         gcaggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGCTTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAG                                                          <  NB501016:230:HFVJLAFXY:3:11405:19154:3116/123‑1 (MQ=60)
                                                                                         gcaggttgacggttatccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGA                                                           <  NB501016:230:HFVJLAFXY:3:11403:26158:14422/122‑1 (MQ=60)
                                                                                         gcagggtgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAG                                                            <  NB501016:230:HFVJLAFXY:1:21106:14617:6344/121‑1 (MQ=60)
                                                                                          caggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGG                                                         <  NB501016:230:HFVJLAFXY:2:21207:26453:8035/124‑1 (MQ=60)
                                                                                          caggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGG                                                         <  NB501016:230:HFVJLAFXY:2:11104:5825:6888/124‑1 (MQ=60)
                                                                                          caggttgacggttttccaccaccgcacGCCGGGACCATCACCGGGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGG                                                         <  NB501016:230:HFVJLAFXY:1:11302:9418:7419/124‑1 (MQ=60)
                                                                                           aggttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGT                                                        <  NB501016:230:HFVJLAFXY:2:21312:4243:5300/125‑1 (MQ=60)
                                                                                             gttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGCAGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGG                                                      >  NB501016:230:HFVJLAFXY:2:21108:19456:14997/25‑151 (MQ=60)
                                                                                             gttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGG                                                      >  NB501016:230:HFVJLAFXY:4:11507:5688:18070/25‑151 (MQ=60)
                                                                                             gttgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACCCTGAATGATTTTCTCTGAGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGG                                                      <  NB501016:230:HFVJLAFXY:3:11604:3555:17664/127‑1 (MQ=60)
                                                                                               tgacggttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACATCCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgcgtcgtct                                                                             <  NB501016:230:HFVJLAFXY:3:21602:14852:19559/104‑14 (MQ=60)
                                                                                                    gttttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTA                                                >  NB501016:230:HFVJLAFXY:4:21506:9017:17972/18‑150 (MQ=60)
                                                                                                     ttttccaccatcgcacGCCGGGACCATCATCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAAC                                              >  NB501016:230:HFVJLAFXY:2:21302:15406:3593/17‑151 (MQ=60)
                                                                                                      tttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACG                                             >  NB501016:230:HFVJLAFXY:1:11103:6330:6561/16‑151 (MQ=60)
                                                                                                      tttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAAC                                              <  NB501016:230:HFVJLAFXY:2:21205:17913:12667/135‑1 (MQ=60)
                                                                                                       ttcctccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACG                                             <  NB501016:230:HFVJLAFXY:4:21610:3914:13670/136‑1 (MQ=60)
                                                                                                       ttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACG                                             <  NB501016:230:HFVJLAFXY:1:11202:11553:12344/136‑1 (MQ=60)
                                                                                                       ttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACG                                             <  NB501016:230:HFVJLAFXY:3:21611:17400:12255/136‑1 (MQ=60)
                                                                                                       ttccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCGGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGCACTGATGGTGGAAGAGGTGGCGCGTAACGC                                            >  NB501016:230:HFVJLAFXY:1:21108:18085:6439/15‑151 (MQ=60)
                                                                                                        tccaccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCG                                           >  NB501016:230:HFVJLAFXY:3:21612:10358:14660/14‑151 (MQ=60)
                                                                                                          caccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTC                                         >  NB501016:230:HFVJLAFXY:4:21607:15641:18106/12‑151 (MQ=60)
                                                                                                          caccatcgcacGCCGGGACCATCACAGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTC                                         >  NB501016:230:HFVJLAFXY:1:21110:10558:3411/12‑151 (MQ=60)
                                                                                                           accatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGT‑GAAGAGGTGGCGCGTAACGCGTCCG                                       <  NB501016:230:HFVJLAFXY:4:21505:16333:15863/141‑1 (MQ=60)
                                                                                                           accatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAAC                                                                        >  NB501016:230:HFVJLAFXY:1:21105:14695:10174/11‑119 (MQ=60)
                                                                                                           accaTCACACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgcgtcgtct                                                                             <  NB501016:230:HFVJLAFXY:3:21508:6129:3313/110‑14 (MQ=60)
                                                                                                            ccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCG                                       <  NB501016:230:HFVJLAFXY:3:21404:10042:9314/142‑1 (MQ=60)
                                                                                                            ccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCG                                       >  NB501016:230:HFVJLAFXY:4:21411:18564:12542/10‑151 (MQ=60)
                                                                                                            ccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTG                                                                <  NB501016:230:HFVJLAFXY:1:21307:22352:2590/117‑1 (MQ=60)
                                                                                                            ccatcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGG                                                                                          >  NB501016:230:HFVJLAFXY:4:11607:23962:6277/10‑100 (MQ=60)
                                                                                                             catcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGT                                      <  NB501016:230:HFVJLAFXY:3:21403:14595:16308/143‑1 (MQ=60)
                                                                                                              atcgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATG                                                                   >  NB501016:230:HFVJLAFXY:1:21206:15306:18927/8‑121 (MQ=60)
                                                                                                                cgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTTAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGT                                   <  NB501016:230:HFVJLAFXY:4:21408:22976:2495/146‑1 (MQ=60)
                                                                                                                cgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgctgcgtcgtcttgagatcggaaggagcacacgtctgaactccagtcaccggct                                   >  NB501016:230:HFVJLAFXY:4:21612:13709:9458/6‑96 (MQ=60)
                                                                                                                cgcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGT                                   >  NB501016:230:HFVJLAFXY:3:11607:2238:11323/6‑151 (MQ=60)
                                                                                                                CACATGCCGGGTCCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCATCCGCGGT                                   >  NB501016:230:HFVJLAFXY:2:21201:20979:9591/1‑151 (MQ=60)
                                                                                                                 gcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTG                                  >  NB501016:230:HFVJLAFXY:3:11611:17732:12835/5‑151 (MQ=60)
                                                                                                                 gcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTG                                  <  NB501016:230:HFVJLAFXY:4:21605:5762:11015/147‑1 (MQ=60)
                                                                                                                 gcacGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTG                                  >  NB501016:230:HFVJLAFXY:4:21510:12388:10651/5‑151 (MQ=60)
                                                                                                                  cccGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGAGGATGCCGGGCCGGAGGTGCTGCGTCGTCTTGAACTGCTGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGG                                 <  NB501016:230:HFVJLAFXY:1:11312:5049:14853/148‑1 (MQ=60)
                                                                                                                  caccCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGG                                 <  NB501016:230:HFVJLAFXY:1:11102:1248:9460/147‑1 (MQ=60)
                                                                                                                  CACGCCTGGACCATCACGGTGTATGAATATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTTAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGG                                 <  NB501016:230:HFVJLAFXY:4:21412:23922:12899/151‑1 (MQ=60)
                                                                                                                  CACGCCGGGCCCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGG                                 >  NB501016:230:HFVJLAFXY:3:21512:20525:17196/1‑151 (MQ=60)
                                                                                                                  CACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGG                                 >  NB501016:230:HFVJLAFXY:3:21603:17146:9126/1‑151 (MQ=60)
                                                                                                                  CACGCCGGGACCATCACCGGGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGG                                 <  NB501016:230:HFVJLAFXY:3:21408:19738:2767/151‑1 (MQ=60)
                                                                                                                   ccGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGC                                <  NB501016:230:HFVJLAFXY:1:21103:5277:10237/149‑1 (MQ=60)
                                                                                                                   ACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGC                                <  NB501016:230:HFVJLAFXY:1:21203:2661:16936/151‑1 (MQ=60)
                                                                                                                    CGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGC                                >  NB501016:230:HFVJLAFXY:4:21511:10699:4665/1‑150 (MQ=60)
                                                                                                                    CGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGC                                >  NB501016:230:HFVJLAFXY:3:11602:21851:12404/1‑150 (MQ=60)
                                                                                                                    CGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCG                                                                                                  >  NB501016:230:HFVJLAFXY:3:11509:25947:7326/1‑84 (MQ=60)
                                                                                                                      CCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCAC                              >  NB501016:230:HFVJLAFXY:1:11205:2959:8328/1‑150 (MQ=60)
                                                                                                                      CCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCAC                              >  NB501016:230:HFVJLAFXY:4:11412:14699:6757/1‑150 (MQ=60)
                                                                                                                      CCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCCGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGAGCGTAACGCGTCCGTGGTGGCAC                              >  NB501016:230:HFVJLAFXY:3:11503:22774:16266/1‑150 (MQ=60)
                                                                                                                       ggtttacgTTACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCtg                              <  NB501016:230:HFVJLAFXY:4:21502:7360:5582/141‑3 (MQ=60)
                                                                                                                       CGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAG                            <  NB501016:230:HFVJLAFXY:3:11610:13685:17373/151‑1 (MQ=60)
                                                                                                                       CGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGGGGCACAG                            <  NB501016:230:HFVJLAFXY:3:11508:21736:2677/151‑1 (MQ=60)
                                                                                                                        GGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAAC                                              >  NB501016:230:HFVJLAFXY:1:11310:6256:17604/1‑132 (MQ=60)
                                                                                                                        GGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGGGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCAC                              >  NB501016:230:HFVJLAFXY:3:11612:17787:13663/1‑148 (MQ=60)
                                                                                                                         GGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAG                          <  NB501016:230:HFVJLAFXY:2:21305:26556:4131/151‑1 (MQ=60)
                                                                                                                         GGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAG                            >  NB501016:230:HFVJLAFXY:2:11204:9457:8254/1‑149 (MQ=60)
                                                                                                                         GGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAGGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGGGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAG                          <  NB501016:230:HFVJLAFXY:3:11609:11180:11208/151‑1 (MQ=60)
                                                                                                                            CCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAgtac                       >  NB501016:230:HFVJLAFXY:2:11103:1466:14114/1‑147 (MQ=60)
                                                                                                                            CCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAgtac                       >  NB501016:230:HFVJLAFXY:2:11107:16150:16354/1‑147 (MQ=60)
                                                                                                                            CCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACG                                             <  NB501016:230:HFVJLAFXY:2:11306:22153:15447/129‑1 (MQ=60)
                                                                                                                             CATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGACCGTCCGGATGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACG                      >  NB501016:230:HFVJLAFXY:2:11309:4784:2627/1‑151 (MQ=60)
                                                                                                                             CATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAgtacg                      <  NB501016:230:HFVJLAFXY:1:11201:23375:18439/151‑6 (MQ=60)
                                                                                                                              ATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGG                     >  NB501016:230:HFVJLAFXY:2:11303:15636:6608/1‑151 (MQ=60)
                                                                                                                               TCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGC                    >  NB501016:230:HFVJLAFXY:4:11409:2466:1361/1‑151 (MQ=60)
                                                                                                                               TCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGT                                                 <  NB501016:230:HFVJLAFXY:2:11206:10169:7474/122‑1 (MQ=60)
                                                                                                                                CACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGC                    >  NB501016:230:HFVJLAFXY:2:21203:25470:11139/1‑150 (MQ=60)
                                                                                                                                CACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGC                    <  NB501016:230:HFVJLAFXY:4:11510:18961:20154/150‑1 (MQ=60)
                                                                                                                                 ACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAG                  <  NB501016:230:HFVJLAFXY:2:21311:3501:6517/151‑1 (MQ=60)
                                                                                                                                 ACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGG                                 >  NB501016:230:HFVJLAFXY:3:11606:3949:19382/1‑136 (MQ=60)
                                                                                                                                   CGTGTATGAAGATTCACAACCGGTGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGAC                >  NB501016:230:HFVJLAFXY:2:21106:16588:7623/1‑151 (MQ=60)
                                                                                                                                   CGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGAC                >  NB501016:230:HFVJLAFXY:2:11202:12347:9263/1‑151 (MQ=60)
                                                                                                                                    GTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGtgct                                                                                      <  NB501016:230:HFVJLAFXY:3:11511:17683:11185/80‑5 (MQ=60)
                                                                                                                                     TGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGagatcggaagagcacacggtctgaactccagtcaccggctctgagctcgtatg              >  NB501016:230:HFVJLAFXY:2:11210:4186:3776/1‑98 (MQ=60)
                                                                                                                                     TGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGC              >  NB501016:230:HFVJLAFXY:1:11109:21345:13899/1‑151 (MQ=60)
                                                                                                                                      GTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCG             >  NB501016:230:HFVJLAFXY:3:11504:15494:5483/1‑151 (MQ=60)
                                                                                                                                      GTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGGGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAA                                               <  NB501016:230:HFVJLAFXY:3:21410:22381:15104/117‑1 (MQ=60)
                                                                                                                                       TATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGCTGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCG             >  NB501016:230:HFVJLAFXY:3:11504:24385:9022/1‑150 (MQ=60)
                                                                                                                                       TATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCG             >  NB501016:230:HFVJLAFXY:3:11601:19756:17426/1‑150 (MQ=60)
                                                                                                                                       TATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCG             >  NB501016:230:HFVJLAFXY:4:21602:13283:8372/1‑150 (MQ=60)
                                                                                                                                       TATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGAGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCG             >  NB501016:230:HFVJLAFXY:2:11106:20318:2007/1‑150 (MQ=60)
                                                                                                                                        ATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGG                                                                  <  NB501016:230:HFVJLAFXY:4:11612:2589:1337/96‑1 (MQ=60)
                                                                                                                                        ATGAAGATTCCCAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGA            >  NB501016:230:HFVJLAFXY:2:21301:24261:3582/1‑150 (MQ=60)
                                                                                                                                        ATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGA            >  NB501016:230:HFVJLAFXY:2:11101:17362:18241/1‑150 (MQ=60)
                                                                                                                                        ATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGA            >  NB501016:230:HFVJLAFXY:4:21611:16930:8713/1‑150 (MQ=60)
                                                                                                                                        ATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGA            >  NB501016:230:HFVJLAFXY:4:21409:3710:11768/1‑150 (MQ=60)
                                                                                                                                         TGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGA            >  NB501016:230:HFVJLAFXY:2:11108:3131:12056/1‑149 (MQ=60)
                                                                                                                                         TGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTG                                                                      <  NB501016:230:HFVJLAFXY:1:11111:16072:6580/91‑1 (MQ=47)
                                                                                                                                         TGAAGATTCACAACCGCGGACGCTGAATGATTTTCTCTGTGCCAAGACGGAGGATGAAGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAC          >  NB501016:230:HFVJLAFXY:2:21110:13391:6567/1‑151 (MQ=60)
                                                                                                                                          GAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGA         <  NB501016:230:HFVJLAFXY:4:21508:4120:11841/151‑1 (MQ=60)
                                                                                                                                          GAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAG          <  NB501016:230:HFVJLAFXY:1:21212:18993:11937/150‑1 (MQ=60)
                                                                                                                                          GAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGA            >  NB501016:230:HFVJLAFXY:2:21211:19746:18982/1‑148 (MQ=60)
                                                                                                                                          GAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGA            >  NB501016:230:HFVJLAFXY:1:11101:12758:10134/1‑148 (MQ=60)
                                                                                                                                          GAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGGCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAG          <  NB501016:230:HFVJLAFXY:1:21301:10281:11498/150‑1 (MQ=60)
                                                                                                                                          GAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCAAGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGA         <  NB501016:230:HFVJLAFXY:3:11411:24345:18615/151‑1 (MQ=60)
                                                                                                                                           AAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAA        <  NB501016:230:HFVJLAFXY:2:11312:11713:7275/151‑1 (MQ=60)
                                                                                                                                           AAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAA        <  NB501016:230:HFVJLAFXY:2:11308:10077:6372/151‑1 (MQ=60)
                                                                                                                                           AAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGA         >  NB501016:230:HFVJLAFXY:4:21405:5953:18661/1‑150 (MQ=60)
                                                                                                                                           AAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGGCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAA        <  NB501016:230:HFVJLAFXY:1:21210:6823:6095/151‑1 (MQ=60)
                                                                                                                                            AGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAA       <  NB501016:230:HFVJLAFXY:4:21404:16293:13891/151‑1 (MQ=60)
                                                                                                                                            AGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAA        >  NB501016:230:HFVJLAFXY:1:11203:4269:1509/1‑150 (MQ=60)
                                                                                                                                            AGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGC                                >  NB501016:230:HFVJLAFXY:3:21407:11464:15262/1‑126 (MQ=60)
                                                                                                                                            AGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGG                                 <  NB501016:230:HFVJLAFXY:4:11610:4381:18994/125‑1 (MQ=60)
                                                                                                                                            AGATTCACAACCGGGGACGCGGAATGATTTGCTCTGTGCCATGACGGAGGAAGATGCCCGGCCGGAGGTGCTGCGTCGTCGTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGGGGTGGCACAGAGTACGGCAGACGCGAAGAAA       <  NB501016:230:HFVJLAFXY:3:11501:12931:1290/151‑1 (MQ=60)
                                                                                                                                             GATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAAT      >  NB501016:230:HFVJLAFXY:1:21208:2857:6372/1‑151 (MQ=60)
                                                                                                                                             GATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGAAGGTGGAAGAGGTGGCGCGTAACGCGGCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAac      <  NB501016:230:HFVJLAFXY:4:11512:26397:13450/151‑3 (MQ=60)
                                                                                                                                                 CACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAGC  >  NB501016:230:HFVJLAFXY:1:11308:6360:6897/1‑151 (MQ=60)
                                                                                                                                                 CACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAGC  >  NB501016:230:HFVJLAFXY:4:11503:14691:9568/1‑151 (MQ=60)
                                                                                                                                                 CACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAGC  >  NB501016:230:HFVJLAFXY:1:11112:8252:14855/1‑151 (MQ=60)
                                                                                                                                                 CACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAG   <  NB501016:230:HFVJLAFXY:1:21207:1476:17027/150‑1 (MQ=60)
                                                                                                                                                 CACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAG   <  NB501016:230:HFVJLAFXY:3:21506:12879:16913/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
GTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGC  >  NC_000913/1429145‑1429440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑