Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,429,732 G→A 100% K228K (AAG→AAA)  stfR → Rac prophage; putative tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,429,7320GA98.1% 7367.9 / 39.4 2604K228K (AAG→AAA) stfRRac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base G (24/29);  new base A (1205/1321);  total (1244/1360)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 2605 total aligned reads displayed.

GCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGC‑AAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCC  >  NC_000913/1429586‑1429879
                                                                                                                                                  |                                                                                                                                                    
gCCACTGAAGCGGAAAAAAGTGCCGCAGCCTCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGc                                                                                                                                                    >  7:191489/1‑149 (MQ=255)
  cACTGAAGCGGAAAAAAGTGCCGCAGCTGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGcgt                                                                                                                                                  <  2:33816/149‑3 (MQ=255)
  cACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGcgt                                                                                                                                                  >  4:192758/1‑147 (MQ=255)
  cACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGcgt                                                                                                                                                  <  8:331164/149‑3 (MQ=255)
  cACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGcgt                                                                                                                                                  <  1:287831/149‑3 (MQ=255)
   aCTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTc                                                                                                                                                 <  1:80474/149‑1 (MQ=255)
    cTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCa                                                                                                                                                <  4:262933/149‑1 (MQ=255)
            gaaaaaaGTGCCGCAGCCTCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGc                                                                                                                                        <  5:269230/147‑1 (MQ=255)
            gaaaaaaGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGACACGAATGCTGCAGCGTCCCAACCATCAGCCGCCACGTCTGCATCCACCGCGGCCACGAACGCGTCAGAGGCCGc                                                                                                                                        >  1:124438/3‑149 (MQ=255)
              aaaaaGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGcca                                                                                                                                      <  3:274984/149‑3 (MQ=255)
                  aGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCActt                                                                                                                                   >  5:369896/1‑146 (MQ=255)
                  aGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTc                                                                                                                                  >  5:90178/1‑149 (MQ=255)
                  aGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTc                                                                                                                                  <  8:130452/149‑1 (MQ=255)
                          agccgCAGAGGCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACgag                                                                                                                          <  1:376112/145‑3 (MQ=255)
                           gccgCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACgaga                                                                                                                         <  7:306765/146‑1 (MQ=255)
                             cgCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATg                                                                                                                       >  5:343248/2‑149 (MQ=255)
                               cAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCCGCCGCCACGTCTGCCTCCCCCGCGGCCACGACAGCGTCAGAGGCCGCCACTTCAGCACGAGATgcg                                                                                                                     >  3:352397/1‑149 (MQ=255)
                               cAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATgcg                                                                                                                     >  3:79893/1‑149 (MQ=255)
                                agagTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATgcgg                                                                                                                    <  8:185959/149‑1 (MQ=255)
                                agagTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATgcgg                                                                                                                    >  7:159906/1‑149 (MQ=255)
                                agagTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGGCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATgcgg                                                                                                                    <  5:212264/149‑1 (MQ=255)
                                 gagTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATgcggt                                                                                                                   <  2:9356/149‑2 (MQ=255)
                                      cTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGcc                                                                                                               <  3:331009/148‑1 (MQ=255)
                                      cTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATAAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCt                                                                                                              >  7:364601/1‑149 (MQ=255)
                                       tCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCt                                                                                                              >  4:102575/1‑148 (MQ=255)
                                       tCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCt                                                                                                              >  2:126872/1‑148 (MQ=255)
                                         aaaaaaCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCa                                                                                                            >  3:142985/1‑148 (MQ=255)
                                           aaaaCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCCGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCaa                                                                                                           >  3:247690/1‑147 (MQ=255)
                                           aaaaCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCaaaa                                                                                                         <  2:235969/149‑1 (MQ=255)
                                           aaaaCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCaaaa                                                                                                         <  4:351488/149‑1 (MQ=255)
                                           aaaGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCaaaa                                                                                                         <  6:80250/149‑1 (MQ=255)
                                              accccgcggcCAACAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAaagag                                                                                                      <  7:94756/144‑2 (MQ=255)
                                               cgcgGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAaagagg                                                                                                     <  4:295133/149‑3 (MQ=255)
                                               cgcgGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAaagagg                                                                                                     <  1:257252/149‑3 (MQ=255)
                                                  ggcggcCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGcag                                                                                                  >  8:51640/1‑147 (MQ=255)
                                                   gcggcCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGc                                                                                                 <  2:66926/149‑1 (MQ=255)
                                                        caccaGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAat                                                                                           <  6:57797/149‑1 (MQ=255)
                                                         accaGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGc                                                                                                    <  5:162518/140‑1 (MQ=255)
                                                         accaGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatc                                                                                          <  6:166791/149‑1 (MQ=255)
                                                         accaGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAat                                                                                           >  7:333021/1‑148 (MQ=255)
                                                          ccaGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatca                                                                                         >  8:217022/1‑149 (MQ=255)
                                                          ccaGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatca                                                                                         >  1:100362/1‑149 (MQ=255)
                                                           caGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatcat                                                                                        <  7:220005/149‑1 (MQ=255)
                                                           caGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatcat                                                                                        <  8:27244/149‑1 (MQ=255)
                                                           caGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCCTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGGCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatcat                                                                                        >  4:323261/1‑149 (MQ=255)
                                                            aGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatcatc                                                                                       <  3:118269/149‑1 (MQ=255)
                                                            aGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatcatc                                                                                       <  5:49121/149‑1 (MQ=255)
                                                            aGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatcat                                                                                        >  7:124345/1‑148 (MQ=255)
                                                             gTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAatcat                                                                                        >  6:320811/1‑147 (MQ=255)
                                                             gTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAAtcatca                                                                                      <  1:48360/149‑1 (MQ=255)
                                                                ccGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGa                                                                                    >  6:149092/1‑148 (MQ=255)
                                                                 cGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGaaa                                                                                  <  4:46398/149‑1 (MQ=255)
                                                                 cGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGaaa                                                                                  <  7:245121/149‑1 (MQ=255)
                                                                   gTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAaac                                                                                 >  1:199787/1‑148 (MQ=255)
                                                                         cgAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAAtcatca                                                                                      >  3:221452/1‑137 (MQ=255)
                                                                               cGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCAtcatca                                                                    <  8:79001/149‑1 (MQ=255)
                                                                                gTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCatcatc                                                                     >  1:173532/1‑147 (MQ=255)
                                                                                gTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCaa                                                                   <  3:41584/149‑1 (MQ=255)
                                                                                   aGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAgtg                                                                >  5:323303/1‑147 (MQ=255)
                                                                                   aGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAgtg                                                                >  4:167988/1‑147 (MQ=255)
                                                                                     aaaCGAATGCTGCAGCGTCAAAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGcc                                                              <  6:120457/149‑1 (MQ=255)
                                                                                          aaTGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCggtcg                                                         >  4:223664/1‑147 (MQ=255)
                                                                                            tgctgcAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTg                                                       <  3:299202/149‑1 (MQ=255)
                                                                                              ctgcAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGc                                                      <  2:27977/145‑1 (MQ=255)
                                                                                              ctgcAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGc                                                      <  2:310095/145‑1 (MQ=255)
                                                                                              ctgcAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGc                                                      <  5:243462/145‑1 (MQ=255)
                                                                                              ctgcAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCa                                                     <  1:233191/146‑1 (MQ=255)
                                                                                                 cAGCGTCACAACAATCAGCCGCCATGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGc                                                   <  4:75747/148‑1 (MQ=255)
                                                                                                  aGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCtt                                                 >  2:391252/1‑149 (MQ=255)
                                                                                                     gTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCt                                              >  5:70405/1‑149 (MQ=255)
                                                                                                      tCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCt                                              >  1:348354/1‑149 (MQ=255)
                                                                                                        acaaCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCgg                                           >  3:188828/4‑149 (MQ=255)
                                                                                                        acaaCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCgg                                           <  3:167988/146‑1 (MQ=255)
                                                                                                         caaCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGccg                                                             <  6:265636/128‑2 (MQ=255)
                                                                                                         caaCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCg                                            <  6:346942/145‑1 (MQ=255)
                                                                                                           aCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAAC‑AAAATCATCAGAaacgaa                                                                              <  7:395977/111‑1 (MQ=255)
                                                                                                            cAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGcatcat                                                                      >  8:375948/1‑118 (MQ=255)
                                                                                                                     gcCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAg                               >  6:298347/1‑148 (MQ=255)
                                                                                                                      ccACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAga                              >  7:70919/1‑147 (MQ=255)
                                                                                                                        acgtCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGaaaa                           <  2:365908/146‑1 (MQ=255)
                                                                                                                        acgtCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCAGCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGaaaa                           <  5:136461/146‑1 (MQ=255)
                                                                                                                          gtCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAg                                                    >  8:16158/2‑122 (MQ=255)
                                                                                                                          gtCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAAtt                         <  6:19049/148‑1 (MQ=255)
                                                                                                                               cctCCACCGCGGCCACGAAAGCGTCGGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgc                     >  6:382740/3‑148 (MQ=255)
                                                                                                                               cctCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTct                       <  8:300785/144‑2 (MQ=255)
                                                                                                                                 tCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccag                  >  2:211174/1‑146 (MQ=255)
                                                                                                                                 tCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccag                  >  2:188986/1‑146 (MQ=255)
                                                                                                                                 tCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccag                  >  6:99113/1‑146 (MQ=255)
                                                                                                                                   cACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCatcatc                                                                     >  5:184029/1‑96 (MQ=255)
                                                                                                                                   cACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccaggg                <  8:242611/149‑6 (MQ=255)
                                                                                                                                     ccGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGggcg              >  4:134152/1‑149 (MQ=255)
                                                                                                                                     ccGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGggcg              <  1:147716/149‑1 (MQ=255)
                                                                                                                                      cgcgGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGggcgg             >  2:15599/1‑149 (MQ=255)
                                                                                                                                       gcgGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGggcggc            <  6:225008/149‑1 (MQ=255)
                                                                                                                                         ggccACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCaa          <  8:354449/147‑1 (MQ=255)
                                                                                                                                           ccACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCaaaa        <  1:319391/149‑1 (MQ=255)
                                                                                                                                             aCGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCTAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAAc      <  5:105849/149‑1 (MQ=255)
                                                                                                                                             aCGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAAc      >  5:300111/1‑149 (MQ=255)
                                                                                                                                               gAAAGCGTCAGAGGCCACCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGC‑AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGt    >  7:277067/1‑149 (MQ=255)
                                                                                                                                                 aGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGC‑AAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTcc  <  2:10261/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGC‑AAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCC  >  NC_000913/1429586‑1429879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 679 total aligned reads displayed.
AAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCG  >  NC_000913/1429582‑1429880
                                                                                                                                                      |                                                                                                                                                    
 aaggccactgaatcggaaaaaattgccgcagccGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGGGCGGCGAAAACGTCACAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAG                                                                                                                                                     <  NB501016:230:HFVJLAFXY:4:11605:7021:18498/118‑1 (MQ=60)
 AAGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGTGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAG                                                                                                                                                     <  NB501016:230:HFVJLAFXY:1:11109:15768:5774/151‑1 (MQ=60)
 AAGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCCCGTCTGCCTCCAACGCGGCCACGAACG                                                                                                                                                     >  NB501016:230:HFVJLAFXY:2:21202:10028:3489/1‑151 (MQ=60)
 AAGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAG                                                                                                                                                     <  NB501016:230:HFVJLAFXY:3:21505:7724:9406/151‑1 (MQ=60)
 AAGGCCACTGAAGCGGAAAAAAGGGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAG                                                                                                                                                     <  NB501016:230:HFVJLAFXY:3:21402:18044:1151/151‑1 (MQ=60)
  AGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGC                                                                                                                                                    <  NB501016:230:HFVJLAFXY:2:21201:17139:15663/151‑1 (MQ=60)
  AGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGC                                                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11607:4619:7043/151‑1 (MQ=60)
   GGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCA                                                                                                                                                   <  NB501016:230:HFVJLAFXY:4:21412:16332:16966/151‑1 (MQ=60)
     CCACTGAAGCGGAAAAAAGTGCCGCAGCTGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGTGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 <  NB501016:230:HFVJLAFXY:3:11406:2563:2776/151‑1 (MQ=60)
     CCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCCGCGTCACAACAATCAGCCGCCACGTCTGCCGCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:2:11109:19340:1769/1‑151 (MQ=60)
     CCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 <  NB501016:230:HFVJLAFXY:1:21204:12832:7939/151‑1 (MQ=60)
     CCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 <  NB501016:230:HFVJLAFXY:4:21504:3067:11101/151‑1 (MQ=60)
     CCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCAGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:3:21404:17640:7374/1‑151 (MQ=60)
      CACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCA                                                                                                                                                <  NB501016:230:HFVJLAFXY:1:11101:15864:12016/151‑1 (MQ=60)
      CACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCA                                                                                                                                                <  NB501016:230:HFVJLAFXY:4:11405:3251:14826/151‑1 (MQ=60)
       ACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGTAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAG                                                                                                                                               <  NB501016:230:HFVJLAFXY:4:21502:8629:18553/151‑1 (MQ=60)
       ACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACACCAATCAGCCGCCCCGTCTGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:3:11510:13772:10459/1‑149 (MQ=60)
       ACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAG                                                                                                                                               <  NB501016:230:HFVJLAFXY:3:11502:13820:9951/151‑1 (MQ=60)
       ACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAG                                                                                                                                               <  NB501016:230:HFVJLAFXY:4:11412:17915:4890/151‑1 (MQ=60)
       ACTGAAGCGGAAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11508:11917:10834/1‑149 (MQ=60)
       ACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11411:2721:7845/1‑149 (MQ=60)
        ctgaagcggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:1:11204:7575:6962/11‑148 (MQ=60)
        ctgaagcggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:2:21109:7906:5796/11‑148 (MQ=60)
        ctgaagcggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:1:21102:17892:15062/11‑148 (MQ=60)
        CTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAG                                                                                                                                                      <  NB501016:230:HFVJLAFXY:4:21609:15379:19232/143‑1 (MQ=60)
          gaagcggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGG                                                                                                                                            <  NB501016:230:HFVJLAFXY:1:21201:23819:8179/143‑1 (MQ=60)
          gaagcggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGG                                                                                                                                            <  NB501016:230:HFVJLAFXY:4:21607:2292:10176/143‑1 (MQ=60)
          gaagcggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGG                                                                                                                                            <  NB501016:230:HFVJLAFXY:2:11112:21885:7051/143‑1 (MQ=60)
          gaagcggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGA                                                                                                                                              <  NB501016:230:HFVJLAFXY:1:11209:3315:15449/141‑1 (MQ=60)
          caagcagaagacggcatacgagatcatagccggtgactgagttcagacgtgtgctcttccgatctGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGG                                                                                                                                            <  NB501016:230:HFVJLAFXY:4:11605:17490:14184/86‑1 (MQ=48)
           aagcggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTGAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCCCCGCGGCCACGAAAGCGTCAGAGGC                                                                                                                                           >  NB501016:230:HFVJLAFXY:2:11206:20476:15984/8‑151 (MQ=60)
           aagcggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGC                                                                                                                                           >  NB501016:230:HFVJLAFXY:4:21606:15865:7946/8‑151 (MQ=60)
              cggaAAAAAGTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTC                                                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11507:22808:11705/5‑142 (MQ=60)
                 CAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGAC                                                                                                                                     <  NB501016:230:HFVJLAFXY:2:11108:22729:8100/151‑1 (MQ=60)
                      AGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGG                                                                                                                                <  NB501016:230:HFVJLAFXY:2:11102:24049:12430/151‑1 (MQ=60)
                       GTGCCGCAGCCGCAGAGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGC                                                                                                                                           <  NB501016:230:HFVJLAFXY:3:21403:20802:4901/139‑1 (MQ=60)
                         GCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCC                                                                                                                             >  NB501016:230:HFVJLAFXY:3:11608:7967:16722/1‑151 (MQ=60)
                                         CCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGCCCTCGGCCCGGGATGCGG                                                                                                                    >  NB501016:230:HFVJLAFXY:4:21512:16316:13611/1‑144 (MQ=60)
                                         CCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCG                                                                                                                                       >  NB501016:230:HFVJLAFXY:3:11610:3107:2603/1‑125 (MQ=60)
                                         CCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGGGATGCGGTGGCCTC                                                                                                             >  NB501016:230:HFVJLAFXY:4:21611:3256:17803/1‑151 (MQ=60)
                                          CTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGG                                                                                                                                            >  NB501016:230:HFVJLAFXY:2:21101:15686:1440/1‑119 (MQ=60)
                                            CAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGA                                                                                                                                              >  NB501016:230:HFVJLAFXY:3:21409:11797:2461/1‑115 (MQ=60)
                                            CAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAA                                                                                                                                                      >  NB501016:230:HFVJLAFXY:1:21312:10801:3362/1‑107 (MQ=60)
                                              AAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAG                                                                                                        <  NB501016:230:HFVJLAFXY:1:11312:25562:9901/151‑1 (MQ=60)
                                               AAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGA                                                                                                       <  NB501016:230:HFVJLAFXY:1:21109:11772:8831/151‑1 (MQ=60)
                                                  ACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGC                                                                                                    <  NB501016:230:HFVJLAFXY:4:21605:17661:19717/151‑1 (MQ=60)
                                                  ACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGC                                                                                                    <  NB501016:230:HFVJLAFXY:1:11301:15771:1862/151‑1 (MQ=60)
                                                  ACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGC                                                                                                    <  NB501016:230:HFVJLAFXY:2:11106:18871:3103/151‑1 (MQ=60)
                                                  ACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGC                                                                                                    <  NB501016:230:HFVJLAFXY:2:11104:19351:17218/151‑1 (MQ=60)
                                                  ACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGC                                                                                                    <  NB501016:230:HFVJLAFXY:4:21505:8908:12840/151‑1 (MQ=60)
                                                        CGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAA                                                                                               >  NB501016:230:HFVJLAFXY:3:21512:18288:5504/1‑150 (MQ=60)
                                                        CGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAA                                                                                               >  NB501016:230:HFVJLAFXY:3:11409:13754:12078/1‑150 (MQ=60)
                                                         GGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACACCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAA                                                                                              >  NB501016:230:HFVJLAFXY:4:21408:26736:1270/1‑150 (MQ=50)
                                                         GGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAA                                                                                             <  NB501016:230:HFVJLAFXY:2:21303:11334:4621/151‑1 (MQ=58)
                                                          GCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAAT                                                                                            >  NB501016:230:HFVJLAFXY:4:21409:5309:3650/1‑151 (MQ=56)
                                                           CCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATC                                                                                           >  NB501016:230:HFVJLAFXY:4:11505:14605:10109/1‑151 (MQ=54)
                                                           CCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATC                                                                                           <  NB501016:230:HFVJLAFXY:4:21401:25251:6804/151‑1 (MQ=54)
                                                           CCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATC                                                                                           >  NB501016:230:HFVJLAFXY:2:11312:3814:15928/1‑151 (MQ=54)
                                                           CCACCAGTGCCGGGGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATC                                                                                           <  NB501016:230:HFVJLAFXY:3:11402:9343:9869/151‑1 (MQ=47)
                                                            CACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCA                                                                                          <  NB501016:230:HFVJLAFXY:3:11411:25338:10533/151‑1 (MQ=54)
                                                            CACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATC                                                                                           >  NB501016:230:HFVJLAFXY:4:21502:13801:9078/1‑150 (MQ=53)
                                                            CACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATC                                                                                           >  NB501016:230:HFVJLAFXY:2:21112:13361:13642/1‑150 (MQ=53)
                                                            CACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATC                                                                                           >  NB501016:230:HFVJLAFXY:4:21512:2500:12729/1‑150 (MQ=53)
                                                             ACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCAT                                                                                         >  NB501016:230:HFVJLAFXY:3:21612:7510:8115/1‑151 (MQ=54)
                                                              CCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATC                                                                                        <  NB501016:230:HFVJLAFXY:4:11502:6442:11490/151‑1 (MQ=54)
                                                              CCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATC                                                                                        <  NB501016:230:HFVJLAFXY:4:21403:10360:14035/151‑1 (MQ=54)
                                                              CCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATC                                                                                        <  NB501016:230:HFVJLAFXY:4:21403:4219:19732/151‑1 (MQ=54)
                                                              CCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGA                                                                                                       <  NB501016:230:HFVJLAFXY:2:11110:5214:5551/136‑1 (MQ=54)
                                                              CCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCAACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATC                                                                                        <  NB501016:230:HFVJLAFXY:1:11302:2507:1382/151‑1 (MQ=47)
                                                               CAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCA                                                                                       <  NB501016:230:HFVJLAFXY:2:21211:19905:4801/151‑1 (MQ=54)
                                                               CAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCA                                                                                       <  NB501016:230:HFVJLAFXY:3:21406:7910:12142/151‑1 (MQ=54)
                                                               CAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCA                                                                                       <  NB501016:230:HFVJLAFXY:2:21312:15439:17111/151‑1 (MQ=54)
                                                               CAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCA                                                                                       <  NB501016:230:HFVJLAFXY:3:11412:4289:5031/151‑1 (MQ=54)
                                                               CAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCA                                                                                       <  NB501016:230:HFVJLAFXY:1:21101:2562:18585/151‑1 (MQ=54)
                                                               CAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCA                                                                                       <  NB501016:230:HFVJLAFXY:3:21610:18882:4854/151‑1 (MQ=54)
                                                               CAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAAGCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCA                                                                                       <  NB501016:230:HFVJLAFXY:2:11301:24233:2203/151‑1 (MQ=47)
                                                               CAGTGCCGGTGCGGCGAAAACGGCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTGCAGCACGAGATGCGGGGGCCTCAAAAGAGGCAGCAAAATCATCA                                                                                       <  NB501016:230:HFVJLAFXY:2:11209:14489:3382/151‑1 (MQ=40)
                                                                AGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAG                                                                                      <  NB501016:230:HFVJLAFXY:1:11101:3596:11987/151‑1 (MQ=54)
                                                                 GTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGA                                                                                     <  NB501016:230:HFVJLAFXY:1:21211:18643:13859/151‑1 (MQ=54)
                                                                 GTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGA                                                                                     <  NB501016:230:HFVJLAFXY:1:11303:2801:13527/151‑1 (MQ=54)
                                                                  TGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAAATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGA                                                                                     >  NB501016:230:HFVJLAFXY:4:11504:1347:7868/1‑150 (MQ=45)
                                                                    CCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAAC                                                                                  <  NB501016:230:HFVJLAFXY:2:11207:15491:1115/151‑1 (MQ=54)
                                                                    CCGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAAC                                                                                  <  NB501016:230:HFVJLAFXY:2:21306:5682:12358/151‑1 (MQ=54)
                                                                     CGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACG                                                                                 <  NB501016:230:HFVJLAFXY:2:11210:8309:15775/151‑1 (MQ=54)
                                                                     CGGTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACG                                                                                 <  NB501016:230:HFVJLAFXY:4:11505:26091:5248/151‑1 (MQ=54)
                                                                       GTGCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAA                                                                               <  NB501016:230:HFVJLAFXY:2:21210:1881:7160/151‑1 (MQ=54)
                                                                         GCGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACG                                                                             <  NB501016:230:HFVJLAFXY:3:21608:13810:8604/151‑1 (MQ=54)
                                                                          CGGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGC                                                                            <  NB501016:230:HFVJLAFXY:2:21106:14968:9535/151‑1 (MQ=54)
                                                                           gctcttccgatctGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGC                                                                            >  NB501016:230:HFVJLAFXY:2:11307:9841:18103/14‑150 (MQ=36)
                                                                           GGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGC                                                                            >  NB501016:230:HFVJLAFXY:3:11605:22401:15295/1‑150 (MQ=53)
                                                                           GGCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAA                                                                                             <  NB501016:230:HFVJLAFXY:4:21611:1582:5926/133‑1 (MQ=33)
                                                                            GCGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCAT                                                                          <  NB501016:230:HFVJLAFXY:4:11501:18457:6582/151‑1 (MQ=54)
                                                                             CGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAATAGGCAGCAAAATCATCAGAAACGAACGCATC                                                                         <  NB501016:230:HFVJLAFXY:1:21311:1239:1119/151‑1 (MQ=47)
                                                                             CGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATC                                                                         >  NB501016:230:HFVJLAFXY:3:21411:20424:7889/1‑151 (MQ=54)
                                                                             CGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATC                                                                         <  NB501016:230:HFVJLAFXY:1:21112:4564:7664/151‑1 (MQ=54)
                                                                             CGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATC                                                                         >  NB501016:230:HFVJLAFXY:4:11601:13420:14182/1‑151 (MQ=54)
                                                                             CGAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGACGCCACTTCAGCACGAGATGCCGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATC                                                                         >  NB501016:230:HFVJLAFXY:3:11405:5853:4034/1‑151 (MQ=39)
                                                                              GAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCA                                                                        <  NB501016:230:HFVJLAFXY:3:21401:4540:15252/151‑1 (MQ=54)
                                                                              GAAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATC                                                                         >  NB501016:230:HFVJLAFXY:4:21407:23514:14861/1‑150 (MQ=53)
                                                                               AAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCAT                                                                       <  NB501016:230:HFVJLAFXY:3:11606:8675:6676/151‑1 (MQ=54)
                                                                               AAAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCAT                                                                       <  NB501016:230:HFVJLAFXY:2:21309:9186:15135/151‑1 (MQ=54)
                                                                                AAACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATC                                                                      <  NB501016:230:HFVJLAFXY:3:11512:8731:11588/151‑1 (MQ=54)
                                                                                 AACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCA                                                                     <  NB501016:230:HFVJLAFXY:1:21111:17425:12047/151‑1 (MQ=54)
                                                                                  ACGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAAC                                                                              >  NB501016:230:HFVJLAFXY:2:11303:10086:11078/1‑141 (MQ=41)
                                                                                   CGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAG                                                                   <  NB501016:230:HFVJLAFXY:3:11504:26057:7788/151‑1 (MQ=54)
                                                                                   CGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAG                                                                   <  NB501016:230:HFVJLAFXY:2:11205:18366:17585/151‑1 (MQ=54)
                                                                                   CGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAG                                                                   <  NB501016:230:HFVJLAFXY:4:11609:14171:13216/151‑1 (MQ=54)
                                                                                   CGTCAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCA                                                                     >  NB501016:230:HFVJLAFXY:2:11306:26758:6246/1‑149 (MQ=52)
                                                                                      CAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGC                                                                <  NB501016:230:HFVJLAFXY:4:11402:11197:18295/151‑1 (MQ=50)
                                                                                      CAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGC                                                                <  NB501016:230:HFVJLAFXY:3:21510:22005:12954/151‑1 (MQ=50)
                                                                                      CAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGC                                                                >  NB501016:230:HFVJLAFXY:3:11602:14498:13924/1‑151 (MQ=50)
                                                                                      CAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGC                                                                <  NB501016:230:HFVJLAFXY:4:11510:16058:9604/151‑1 (MQ=50)
                                                                                      CAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGC                                                                >  NB501016:230:HFVJLAFXY:3:11503:18336:14890/1‑151 (MQ=50)
                                                                                      CAGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTG                                                                 <  NB501016:230:HFVJLAFXY:3:21601:20680:5651/150‑1 (MQ=50)
                                                                                       AGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCC                                                               >  NB501016:230:HFVJLAFXY:4:21510:13348:13878/1‑151 (MQ=48)
                                                                                       AGAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCC                                                               >  NB501016:230:HFVJLAFXY:1:21205:19074:5800/1‑151 (MQ=48)
                                                                                        GAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGTCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccg                                                              >  NB501016:230:HFVJLAFXY:1:11308:20000:11953/1‑146 (MQ=40)
                                                                                        GAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccg                                                              >  NB501016:230:HFVJLAFXY:1:11206:4198:13947/1‑146 (MQ=48)
                                                                                        GAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccg                                                              >  NB501016:230:HFVJLAFXY:1:11204:19393:7237/1‑146 (MQ=48)
                                                                                        GAAACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccg                                                              >  NB501016:230:HFVJLAFXY:1:21207:6295:5113/1‑146 (MQ=48)
                                                                                        GAAACGAATGCTGCAGCGTCACAACAATCACCCGCCACGTCTGCATCCACCGCGACCACGAAACCGTCAGACGCCCCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAAAGCAACATCAAGtgcac                                                              >  NB501016:230:HFVJLAFXY:1:21302:2454:15452/1‑146 (MQ=26)
                                                                                          AACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGTAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGT                                                            >  NB501016:230:HFVJLAFXY:3:21602:9641:5258/1‑151 (MQ=36)
                                                                                          AACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGT                                                            >  NB501016:230:HFVJLAFXY:4:21406:11148:1701/1‑151 (MQ=43)
                                                                                          AACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGT                                                            <  NB501016:230:HFVJLAFXY:1:21202:7587:14366/151‑1 (MQ=43)
                                                                                          AACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGT                                                            <  NB501016:230:HFVJLAFXY:2:21308:21636:15729/151‑1 (MQ=43)
                                                                                          AACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGT                                                            >  NB501016:230:HFVJLAFXY:3:11507:24027:12882/1‑151 (MQ=43)
                                                                                           ACGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccggtc                                                           >  NB501016:230:HFVJLAFXY:2:11109:9045:20305/1‑143 (MQ=44)
                                                                                            CGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccggtcg                                                          <  NB501016:230:HFVJLAFXY:1:11110:5631:2928/151‑10 (MQ=42)
                                                                                            CGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccggtcg                                                          <  NB501016:230:HFVJLAFXY:4:11409:17368:19684/151‑10 (MQ=42)
                                                                                            CGAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCA                                                                     >  NB501016:230:HFVJLAFXY:4:21601:19781:11210/1‑140 (MQ=40)
                                                                                             GAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCCCCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccggtcgt                                                         >  NB501016:230:HFVJLAFXY:1:21104:4698:13600/1‑141 (MQ=33)
                                                                                             GAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccggtcgt                                                         >  NB501016:230:HFVJLAFXY:1:11102:23956:7588/1‑141 (MQ=41)
                                                                                             GAATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTG                                                                 <  NB501016:230:HFVJLAFXY:3:11403:7691:19188/143‑1 (MQ=41)
                                                                                                 GCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCA                                                      <  NB501016:230:HFVJLAFXY:3:21506:7029:5084/150‑1 (MQ=35)
                                                                                                 GCTGCAGCGGCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCA                                                      <  NB501016:230:HFVJLAFXY:3:11603:24110:13318/150‑1 (MQ=28)
                                                                                                  CTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGC                                                    >  NB501016:230:HFVJLAFXY:2:11207:3085:6721/1‑151 (MQ=35)
                                                                                                  CTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGC                                                    >  NB501016:230:HFVJLAFXY:4:21604:22547:6699/1‑151 (MQ=35)
                                                                                                   TGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCT                                                   >  NB501016:230:HFVJLAFXY:3:11405:16072:16386/1‑151 (MQ=36)
                                                                                                   TGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCT                                                   >  NB501016:230:HFVJLAFXY:2:11309:19720:16033/1‑151 (MQ=36)
                                                                                                   TGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCT                                                   >  NB501016:230:HFVJLAFXY:2:21305:15713:19840/1‑151 (MQ=36)
                                                                                                   TGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGC                                                    >  NB501016:230:HFVJLAFXY:4:21610:18892:9980/1‑150 (MQ=35)
                                                                                                    GCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTT                                                  <  NB501016:230:HFVJLAFXY:1:21209:7664:17469/151‑1 (MQ=38)
                                                                                                    GCAGCGTCACAACAATCAGCCGCCACGTCTGCCACCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTT                                                  >  NB501016:230:HFVJLAFXY:4:21410:3866:19695/1‑151 (MQ=31)
                                                                                                     CAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGC                                                       >  NB501016:230:HFVJLAFXY:3:11412:13865:11809/1‑145 (MQ=36)
                                                                                                      AGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCC                                                >  NB501016:230:HFVJLAFXY:1:21307:16434:12990/1‑151 (MQ=39)
                                                                                                       GCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCACAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCT                                               >  NB501016:230:HFVJLAFXY:3:21411:3667:8992/1‑151 (MQ=32)
                                                                                                        CGTCACAACAATCAGCCGCGACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATTCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTC                                              <  NB501016:230:HFVJLAFXY:3:21408:16695:16703/151‑1 (MQ=29)
                                                                                                        CGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGtgccggtcctgaagcttcaac                                              >  NB501016:230:HFVJLAFXY:4:21507:16588:18367/1‑130 (MQ=34)
                                                                                                        CGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAAC                                                                              >  NB501016:230:HFVJLAFXY:1:11108:13265:3594/1‑119 (MQ=19)
                                                                                                         GTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTTCTCG                                             <  NB501016:230:HFVJLAFXY:2:11310:6946:13988/151‑1 (MQ=32)
                                                                                                         GTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCG                                             >  NB501016:230:HFVJLAFXY:4:11408:22738:9357/1‑151 (MQ=39)
                                                                                                         GTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCG                                             >  NB501016:230:HFVJLAFXY:1:11310:19221:19762/1‑151 (MQ=39)
                                                                                                         GTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCG                                             >  NB501016:230:HFVJLAFXY:1:21303:9743:13329/1‑151 (MQ=39)
                                                                                                         GTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCG                                             >  NB501016:230:HFVJLAFXY:3:21508:20820:15547/1‑151 (MQ=39)
                                                                                                         GTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCC                                                               >  NB501016:230:HFVJLAFXY:2:11203:13523:4519/1‑133 (MQ=32)
                                                                                                          TCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAACGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGG                                            >  NB501016:230:HFVJLAFXY:2:11304:6889:10319/1‑151 (MQ=39)
                                                                                                           CACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTTCTCGGC                                           >  NB501016:230:HFVJLAFXY:2:21103:13973:10670/1‑151 (MQ=33)
                                                                                                           CACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGC                                           <  NB501016:230:HFVJLAFXY:4:11603:14359:14492/151‑1 (MQ=40)
                                                                                                           CACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGC                                           <  NB501016:230:HFVJLAFXY:1:11210:24172:8270/151‑1 (MQ=40)
                                                                                                           CACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGC                                           >  NB501016:230:HFVJLAFXY:4:11406:5182:17660/1‑151 (MQ=40)
                                                                                                           CACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGC                                           >  NB501016:230:HFVJLAFXY:3:21503:4837:12024/1‑151 (MQ=40)
                                                                                                           CACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGG                                            <  NB501016:230:HFVJLAFXY:1:11203:6585:10575/150‑1 (MQ=39)
                                                                                                           CACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGG                                            <  NB501016:230:HFVJLAFXY:4:21404:9143:14700/150‑1 (MQ=39)
                                                                                                           CACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCACGGC                                           >  NB501016:230:HFVJLAFXY:4:21508:14372:12482/1‑151 (MQ=33)
                                                                                                           CACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACG                                                                             <  NB501016:230:HFVJLAFXY:4:11604:2900:4325/117‑1 (MQ=18)
                                                                                                            ACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGG                                            >  NB501016:230:HFVJLAFXY:3:21606:10884:7480/1‑149 (MQ=39)
                                                                                                             CTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCA                                          >  NB501016:230:HFVJLAFXY:4:11506:16027:17636/1‑150 (MQ=60)
                                                                                                             CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCA                                          >  NB501016:230:HFVJLAFXY:2:21204:2421:14250/1‑150 (MQ=42)
                                                                                                             CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCA                                          >  NB501016:230:HFVJLAFXY:3:11509:24816:12861/1‑150 (MQ=42)
                                                                                                             CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCA                                          >  NB501016:230:HFVJLAFXY:2:21110:7091:18272/1‑150 (MQ=42)
                                                                                                             CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCT                                                   >  NB501016:230:HFVJLAFXY:1:11202:22278:20296/1‑141 (MQ=31)
                                                                                                             CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCA                                                      >  NB501016:230:HFVJLAFXY:3:21508:11540:5369/1‑138 (MQ=30)
                                                                                                             CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACG                                                                             >  NB501016:230:HFVJLAFXY:1:21110:2975:8469/1‑115 (MQ=18)
                                                                                                             CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATCCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCA                                          >  NB501016:230:HFVJLAFXY:1:11211:7153:19692/1‑150 (MQ=35)
                                                                                                              AACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAAC                                        <  NB501016:230:HFVJLAFXY:1:21106:3916:11919/151‑1 (MQ=45)
                                                                                                              AACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCAT                                                                       >  NB501016:230:HFVJLAFXY:1:11105:12695:16428/1‑120 (MQ=26)
                                                                                                               ACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCCCGAGATGCGGTGGCCTCAAAAGAGGCATCAAAATCATCAGAATCGCACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACG                                       >  NB501016:230:HFVJLAFXY:2:21205:24879:14494/1‑151 (MQ=32)
                                                                                                               ACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACG                                       >  NB501016:230:HFVJLAFXY:3:21602:19752:8584/1‑151 (MQ=47)
                                                                                                               ACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTC                                              <  NB501016:230:HFVJLAFXY:1:11112:7789:15703/144‑1 (MQ=37)
                                                                                                               ACAATCAGCAGCCACATCTGCATACACCGCGACCACGAATGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATAAGAAACGCACGCATCATCAAGCGCCAGTAGTCCAGCTTCCTCGGCAACG                                       >  NB501016:230:HFVJLAFXY:4:21602:22137:18574/1‑151 (MQ=60)
                                                                                                                CAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGG                                      >  NB501016:230:HFVJLAFXY:2:21207:16733:14042/1‑151 (MQ=47)
                                                                                                                 AATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGC                                     <  NB501016:230:HFVJLAFXY:4:11404:12417:15808/151‑1 (MQ=47)
                                                                                                                 AATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGC                                     <  NB501016:230:HFVJLAFXY:2:21312:2296:14239/151‑1 (MQ=47)
                                                                                                                     AGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCCACGGCGGC                                  >  NB501016:230:HFVJLAFXY:2:21104:5887:17892/1‑150 (MQ=44)
                                                                                                                     AGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCA                                 <  NB501016:230:HFVJLAFXY:3:11410:22486:6181/151‑1 (MQ=53)
                                                                                                                     AGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGC                                  >  NB501016:230:HFVJLAFXY:1:21301:10562:4916/1‑150 (MQ=51)
                                                                                                                     AGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGC                                  >  NB501016:230:HFVJLAFXY:4:11409:4285:5949/1‑150 (MQ=51)
                                                                                                                     AGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAAC                                        >  NB501016:230:HFVJLAFXY:4:21508:23412:11250/1‑144 (MQ=43)
                                                                                                                         GCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGCACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAA                              >  NB501016:230:HFVJLAFXY:1:21309:17269:11526/1‑150 (MQ=47)
                                                                                                                         GCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCCACGGCGGCAGAA                              >  NB501016:230:HFVJLAFXY:3:11505:13872:20113/1‑150 (MQ=47)
                                                                                                                         GCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAA                             <  NB501016:230:HFVJLAFXY:4:11610:20512:20106/151‑1 (MQ=54)
                                                                                                                         GCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAA                              >  NB501016:230:HFVJLAFXY:2:11209:9098:2588/1‑150 (MQ=55)
                                                                                                                         GCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAA                              >  NB501016:230:HFVJLAFXY:3:11608:21150:7045/1‑150 (MQ=55)
                                                                                                                         GCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAA                              >  NB501016:230:HFVJLAFXY:2:21301:3963:6100/1‑150 (MQ=55)
                                                                                                                          CCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAA                            <  NB501016:230:HFVJLAFXY:3:21604:18615:8353/151‑1 (MQ=54)
                                                                                                                          CCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAA                             >  NB501016:230:HFVJLAFXY:3:21604:7321:1400/1‑150 (MQ=55)
                                                                                                                             CGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACCTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTC                         <  NB501016:230:HFVJLAFXY:1:21308:20481:16726/151‑1 (MQ=59)
                                                                                                                               TCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGC                                     >  NB501016:230:HFVJLAFXY:1:11306:19318:6238/1‑137 (MQ=50)
                                                                                                                                        ACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGGGGCGGCCTCAACAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGG              <  NB501016:230:HFVJLAFXY:3:21611:6857:2345/151‑1 (MQ=60)
                                                                                                                                                    AAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCG  >  NB501016:230:HFVJLAFXY:3:21511:20099:7640/1‑151 (MQ=60)
                                                                                                                                                      |                                                                                                                                                    
AAAGGCCACTGAAGCATCAAAAAGTGCTGCCGCTGCAGAGTCCTCAAAAAGCGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCG  >  NC_000913/1429582‑1429880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑