Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,429,823 A→C 100% S259R (AGT→CGT)  stfR → Rac prophage; putative tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,429,8230AC98.2% 6959.8 / 45.7 2392S259R (AGT→CGT) stfRRac prophage; putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base A (24/23);  new base C (1262/1081);  total (1286/1106)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 2407 total aligned reads displayed.

ATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGC  >  NC_000913/1429677‑1429965
                                                                                                                                                  |                                                                                                                                              
aTGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCggtcgt                                                                                                                                              <  6:193638/149‑1 (MQ=255)
   ctgcAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCa                                                                                                                                           <  1:233191/146‑1 (MQ=255)
      cAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCt                                                                                                                                        >  6:139864/1‑149 (MQ=255)
             acaaCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCgg                                                                                                                                 <  5:254056/146‑1 (MQ=255)
             acaaCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCgg                                                                                                                                 >  2:101906/4‑149 (MQ=255)
             acaaCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGCCGTGCAGCTTCCTCg                                                                                                                                  <  7:212109/145‑1 (MQ=255)
              caaCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGGCGTGCAGCTTCCTCgg                                                                                                                                 >  4:214461/3‑148 (MQ=255)
               aaCAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGc                                                                                                                                >  4:12001/2‑148 (MQ=255)
                 cAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAAc                                                                                                                             >  6:308706/1‑149 (MQ=255)
                  aaTCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAAcg                                                                                                                            >  3:278876/1‑149 (MQ=255)
                  aaTCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGACGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGACCCGGTCGTGCAGCTTCCTCGGCAAcg                                                                                                                            >  8:164942/1‑149 (MQ=255)
                   aTCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAAcgg                                                                                                                           >  2:146765/1‑149 (MQ=255)
                      agccgcCACGTCTGCCTCCACCGCGGCCACGAAAGCGGCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAAcggcgg                                                                                                                        <  2:40781/149‑1 (MQ=255)
                        ccgcCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCa                                                                                                                      <  3:323131/147‑1 (MQ=255)
                           ccACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGCACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAga                                                                                                                    >  1:378653/1‑147 (MQ=255)
                              cgtCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAAt                                                                                                                >  1:141283/3‑149 (MQ=255)
                               gtCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAAtt                                                                                                               >  7:71780/2‑149 (MQ=255)
                                  tGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCg                                                                                                                                  <  6:44257/127‑1 (MQ=255)
                                   gcctCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgc                                                                                                           >  7:272998/4‑149 (MQ=255)
                                   gcctCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgc                                                                                                           >  5:358206/4‑149 (MQ=255)
                                    cctCCACCGCGGCCACGAAAGCGGCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgcc                                                                                                          <  4:68585/147‑2 (MQ=255)
                                     ctCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgc                                                                                                           >  1:122624/2‑147 (MQ=255)
                                      tCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgcc                                                                                                          >  5:329973/1‑146 (MQ=255)
                                       ccACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccagg                                                                                                       >  3:131750/1‑145 (MQ=255)
                                        cACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccaggg                                                                                                      >  1:100674/1‑144 (MQ=255)
                                        cACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccaggg                                                                                                      >  3:857/1‑144 (MQ=255)
                                          ccGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAggcg                                                                                                    <  8:105279/149‑1 (MQ=255)
                                           cgcgGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGggcgg                                                                                                   <  1:262150/149‑1 (MQ=255)
                                             cggccACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGggcggc                                                                                                  >  1:174552/4‑148 (MQ=255)
                                                ccACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCaaaa                                                                                              <  1:352229/149‑1 (MQ=255)
                                                 cACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGCAACGACCGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCaaaaa                                                                                             >  7:152496/1‑149 (MQ=255)
                                                  aCGAAAGCGTCAGAGGCCGCAACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAAc                                                                                            <  3:196809/149‑1 (MQ=255)
                                                    gAAAGCGTCAGAGGCCGCCCCTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGACCGCATCATCAAGTGCCGCTCGTGCAGCTTCCTCGGCAACGGCTGCAGAAAATTCTGCCAGGGCGGCACAAACGt                                                                                          >  2:121104/1‑149 (MQ=255)
                                                    gAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGt                                                                                          <  4:145220/149‑1 (MQ=255)
                                                    gAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGt                                                                                          <  1:50463/149‑1 (MQ=255)
                                                          gtCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCgaga                                                                                    >  2:347094/2‑149 (MQ=255)
                                                           tCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGAc                                                                                   <  5:233780/149‑1 (MQ=255)
                                                             agagGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCATAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACg                                                                                  >  2:178143/1‑148 (MQ=255)
                                                             agagGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGTGGCAAAAACGTCCGAGACGa                                                                                 <  1:283096/149‑1 (MQ=255)
                                                                            agcacgagATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCGTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGtcatct                                                                   <  3:306637/141‑1 (MQ=255)
                                                                              cacgagATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGaa                                                                <  8:290832/144‑1 (MQ=255)
                                                                               ccGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGaaa                                                               <  4:302336/149‑1 (MQ=255)
                                                                               atgagATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGaaa                                                               >  3:100252/5‑149 (MQ=255)
                                                                               acgagATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGaaa                                                               <  8:186644/145‑1 (MQ=255)
                                                                               acgagATGCGGTGGCCTCAAAAGAGGCAGCAAAAGCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGaaa                                                               <  6:108705/145‑1 (MQ=255)
                                                                                cgagATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGaaa                                                               >  8:221672/4‑148 (MQ=255)
                                                                                 gagATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGC‑AAAACGTCCGAGACGAATGCCAGGTCATCTGAAAccg                                                            >  2:240626/3‑147 (MQ=255)
                                                                                  agATGCGGTGGCCTCAAACGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAAcag                                                            <  2:277156/148‑3 (MQ=255)
                                                                                  agATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAAcag                                                            <  4:328539/148‑3 (MQ=255)
                                                                                   gATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAAcag                                                            >  6:332849/1‑146 (MQ=255)
                                                                                    aTGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAAcag                                                            >  5:42080/1‑145 (MQ=255)
                                                                                       cggtggCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCg                                                       <  7:180791/149‑1 (MQ=255)
                                                                                          tggcCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACTTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCggaa                                                    <  7:377558/148‑3 (MQ=255)
                                                                                          tggcCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCggaa                                                    >  5:85977/2‑147 (MQ=255)
                                                                                            gcCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAAcg                                                  <  3:48791/149‑2 (MQ=255)
                                                                                             ccTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAAcgg                                                 <  1:306372/149‑3 (MQ=255)
                                                                                              cTCAAAAGAGGCAGCAAAATCATCAGAACCGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACgga                                                <  5:18593/149‑1 (MQ=255)
                                                                                              cTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAAcg                                                  >  8:249447/1‑146 (MQ=255)
                                                                                              cTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAAcg                                                  >  1:71959/1‑146 (MQ=255)
                                                                                              cTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACgga                                                >  3:228545/1‑149 (MQ=255)
                                                                                              cTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACgga                                                >  8:39868/1‑149 (MQ=255)
                                                                                              cTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACgga                                                <  7:34339/149‑1 (MQ=255)
                                                                                               tCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgcca                                                                                                         <  8:322512/91‑3 (MQ=255)
                                                                                               tCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACggag                                               >  3:255823/1‑149 (MQ=255)
                                                                                               tCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACggag                                               >  3:346837/1‑149 (MQ=255)
                                                                                                 aaaaGAGGCAGCAAAATCATCAGAAACTAACGCATCATCAAGTGCCGGGCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCATAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAgc                                              <  2:370162/148‑1 (MQ=255)
                                                                                                 aaaaGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAgc                                              <  6:168857/148‑1 (MQ=255)
                                                                                                  aaaGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTc                                                                                                                                   >  8:64186/1‑62 (MQ=255)
                                                                                                          agCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGccg                                    >  7:316599/2‑147 (MQ=255)
                                                                                                           gCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGc                                   <  8:366360/149‑1 (MQ=255)
                                                                                                            cAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGaaa                                                               <  4:268596/120‑1 (MQ=255)
                                                                                                             aaaaTCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCgg                                 <  5:280560/149‑1 (MQ=255)
                                                                                                             aaaaTCATCAGAAACGAACGCATCAGCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCgg                                 <  6:91145/149‑1 (MQ=255)
                                                                                                              aaaTCATCAGAAACGCACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGc                                >  8:135155/1‑149 (MQ=255)
                                                                                                               aaTCATCAGAAACGATCGCATCATCAAGTGCCGGTTGTGCAGCTTCCTCGGCAACGGGGGCTGAAAATTCTGCCAGGGCGGCAAAAAAGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCa                               <  2:76919/149‑1 (MQ=255)
                                                                                                               aaTCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCgg                                 >  5:132601/1‑147 (MQ=255)
                                                                                                               aaTCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCgg                                 >  1:201117/1‑147 (MQ=255)
                                                                                                                atcatcAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAg                              <  2:325024/149‑1 (MQ=255)
                                                                                                                   atcaGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAgacg                           <  2:205091/149‑4 (MQ=255)
                                                                                                                   atcaGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAgacg                           <  1:327289/149‑4 (MQ=255)
                                                                                                                    tcaGAAACGCACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGACTCTGCCGCGGCAgacg                           >  6:67367/1‑145 (MQ=255)
                                                                                                                    tcaGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAgacg                           >  5:159833/1‑145 (MQ=255)
                                                                                                                       gAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCaa                        >  5:301566/1‑148 (MQ=255)
                                                                                                                       gAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCaa                        >  4:173759/1‑148 (MQ=255)
                                                                                                                        aaaCGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCaaa                       >  3:163582/1‑148 (MQ=255)
                                                                                                                         aacgaacgCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCaaaaa                     <  7:292040/149‑1 (MQ=255)
                                                                                                                            gaacgCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAg                  >  7:236475/1‑149 (MQ=255)
                                                                                                                             aacgCATCATCGAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGc                 <  6:281282/149‑1 (MQ=255)
                                                                                                                             aacgCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGc                 >  7:46133/1‑149 (MQ=255)
                                                                                                                             aacgCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGc                 <  6:222335/149‑1 (MQ=255)
                                                                                                                              acgCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCg                >  5:6201/1‑149 (MQ=255)
                                                                                                                                 catcatCAAGTGCCGGTCGTGCAGCTTCCTCGGCCCCGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGcg             >  5:214391/1‑148 (MQ=255)
                                                                                                                                  atcatcAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCg                >  7:117834/1‑145 (MQ=255)
                                                                                                                                  atcatcAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGcgg            >  6:358641/1‑147 (MQ=255)
                                                                                                                                   tcatcaAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCggcggc           <  8:342782/149‑1 (MQ=255)
                                                                                                                                     atcaATTGCCGGTCGTGCATCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGGAAAAACGTCCGAGACGAATGCCAGGTCATCGGAAACAGCAGCGGAACGCAGCGCCACTGCCGCGGCAGACGCAAAAACAgcggcggcgc         >  4:236708/1‑148 (MQ=255)
                                                                                                                                     atcaAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAAGGCCAGGGCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAgcggcggcgg         >  6:249875/1‑148 (MQ=255)
                                                                                                                                      tcaAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAgcggcggcggg        >  5:103327/1‑147 (MQ=255)
                                                                                                                                       caAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGa                                                                                 >  2:30185/1‑75 (MQ=255)
                                                                                                                                       caAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAgcggcggcggg        >  4:358310/1‑146 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
ATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGC  >  NC_000913/1429677‑1429965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 1471 total aligned reads displayed.
AATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAG  >  NC_000913/1429676‑1429973
                                                                                                                                                   |                                                                                                                                                      
AATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTG                                                                                                                                                     >  NB501016:230:HFVJLAFXY:4:21509:19893:13711/1‑151 (MQ=38)
 ATGCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGC                                                                                                                                                    <  NB501016:230:HFVJLAFXY:2:21211:21388:16609/151‑1 (MQ=36)
   GCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCA                                                                                                                                                   <  NB501016:230:HFVJLAFXY:3:21506:7029:5084/150‑1 (MQ=35)
   GCTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCA                                                                                                                                                   <  NB501016:230:HFVJLAFXY:3:21410:18632:19498/150‑1 (MQ=35)
     TGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCT                                                                                                                                                >  NB501016:230:HFVJLAFXY:3:11406:2372:11210/1‑151 (MQ=36)
      TCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCT                                                                                                                                                <  NB501016:230:HFVJLAFXY:4:11602:26841:16864/150‑1 (MQ=60)
      GCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCAGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCT                                                                                                                                                <  NB501016:230:HFVJLAFXY:1:21312:17341:3633/150‑1 (MQ=29)
        AGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCC                                                                                                                                             <  NB501016:230:HFVJLAFXY:4:11407:6887:10046/151‑1 (MQ=39)
         GCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCACAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCT                                                                                                                                            >  NB501016:230:HFVJLAFXY:3:21411:3667:8992/1‑151 (MQ=32)
         GCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGGGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCT                                                                                                                                            <  NB501016:230:HFVJLAFXY:3:11405:17606:2230/151‑1 (MQ=39)
          CGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTC                                                                                                                                           >  NB501016:230:HFVJLAFXY:3:11502:18752:9906/1‑151 (MQ=39)
           GTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCG                                                                                                                                          <  NB501016:230:HFVJLAFXY:1:11311:24925:15708/151‑1 (MQ=39)
           GTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCG                                                                                                                                          <  NB501016:230:HFVJLAFXY:3:11505:11503:1750/151‑1 (MQ=39)
              ACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGG                                                                                                                                         >  NB501016:230:HFVJLAFXY:3:21606:10884:7480/1‑149 (MQ=39)
               CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCA                                                                                                                                       >  NB501016:230:HFVJLAFXY:1:21308:11206:7332/1‑150 (MQ=42)
               CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCA                                                                                                                                       >  NB501016:230:HFVJLAFXY:3:21604:5063:9374/1‑150 (MQ=42)
               CAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCA                                                                                                                                                   >  NB501016:230:HFVJLAFXY:3:21508:11540:5369/1‑138 (MQ=30)
                AACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAAC                                                                                                                                     <  NB501016:230:HFVJLAFXY:1:21106:3916:11919/151‑1 (MQ=45)
                AACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAAC                                                                                                                                     >  NB501016:230:HFVJLAFXY:2:21301:1226:14472/1‑151 (MQ=45)
                 ACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACG                                                                                                                                    >  NB501016:230:HFVJLAFXY:2:11109:15819:16570/1‑151 (MQ=47)
                  CAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCACCGG                                                                                                                                   >  NB501016:230:HFVJLAFXY:2:21110:23277:6276/1‑151 (MQ=40)
                  CAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGG                                                                                                                                   >  NB501016:230:HFVJLAFXY:4:11507:7379:11544/1‑151 (MQ=47)
                   AATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGC                                                                                                                                  >  NB501016:230:HFVJLAFXY:3:11410:10422:8263/1‑151 (MQ=47)
                    ATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCG                                                                                                                                 <  NB501016:230:HFVJLAFXY:2:11309:23993:5029/151‑1 (MQ=48)
                       agccgccacggctgcctccaccgcggccacgaaagcgtcagaggacgccacttcagcacgagatgcgttGGCCTCAAAAGAGGCAGGAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCA                                                                                                                              <  NB501016:230:HFVJLAFXY:4:21402:17922:13521/82‑1 (MQ=60)
                        gccgccacgtctgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAG                                                                                                                             <  NB501016:230:HFVJLAFXY:2:21304:25195:9398/91‑1 (MQ=60)
                          cgccacgtctgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAG                                                                                                                                                  >  NB501016:230:HFVJLAFXY:2:11201:10489:13940/59‑128 (MQ=47)
                           GCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGCACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAA                                                                                                                           >  NB501016:230:HFVJLAFXY:1:21309:17269:11526/1‑150 (MQ=47)
                           GCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAA                                                                                                                           >  NB501016:230:HFVJLAFXY:3:11506:2147:8092/1‑150 (MQ=55)
                            CCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAA                                                                                                                          >  NB501016:230:HFVJLAFXY:1:11109:6095:7931/1‑150 (MQ=55)
                             cacgtctgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAAT                                                                                                                        <  NB501016:230:HFVJLAFXY:1:11106:4780:3164/96‑1 (MQ=60)
                             cacgtctgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAAT                                                                                                                        >  NB501016:230:HFVJLAFXY:2:11101:18287:12118/56‑151 (MQ=60)
                             cacgtctgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCAGCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAAT                                                                                                                        <  NB501016:230:HFVJLAFXY:3:11602:3380:16669/96‑1 (MQ=60)
                              acgtctgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATT                                                                                                                       >  NB501016:230:HFVJLAFXY:4:11505:4430:4441/55‑151 (MQ=60)
                               cgtctgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTC                                                                                                                      <  NB501016:230:HFVJLAFXY:4:11609:6604:16444/98‑1 (MQ=60)
                               cgtctgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTC                                                                                                                      >  NB501016:230:HFVJLAFXY:4:21506:10338:13723/54‑151 (MQ=60)
                                 gctgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTG                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11205:7536:18590/100‑1 (MQ=60)
                                 gctgcctccaccgcggccacgaaagcggcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTG                                                                                                                    <  NB501016:230:HFVJLAFXY:1:11307:11848:8692/100‑1 (MQ=60)
                                 TCTGCCTCCACCGCGGCCACGAAAGCGTCAGAGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTG                                                                                                                    >  NB501016:230:HFVJLAFXY:1:21111:12480:13537/1‑151 (MQ=58)
                                   tgcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCGCAAAAGAGGCAGCAAAAGCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGCAAATTCtgcc                                                                                                                  >  NB501016:230:HFVJLAFXY:3:11612:20304:9089/50‑147 (MQ=60)
                                    gcctcccccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGGGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGC                                                                                                                   <  NB501016:230:HFVJLAFXY:4:11607:23645:19853/101‑1 (MQ=60)
                                    gcctccaccgcggccacgaaagcgtcagagggcgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgcc                                                                                                                  <  NB501016:230:HFVJLAFXY:3:11607:7810:4133/102‑5 (MQ=60)
                                    gcctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgcc                                                                                                                  >  NB501016:230:HFVJLAFXY:4:21401:22188:14041/49‑146 (MQ=60)
                                    gcctccaccgcggccacgaaagcggcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgcc                                                                                                                  <  NB501016:230:HFVJLAFXY:1:11107:20658:14433/102‑5 (MQ=60)
                                     cctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGCGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccag                                                                                                                <  NB501016:230:HFVJLAFXY:3:21401:25190:19661/104‑7 (MQ=60)
                                     cctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTG                                                                                                                    <  NB501016:230:HFVJLAFXY:2:21303:16713:7891/100‑1 (MQ=60)
                                      ctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccagg                                                                                                               >  NB501016:230:HFVJLAFXY:1:11210:22350:8610/47‑144 (MQ=60)
                                      ctccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTG                                                                                                                                                     <  NB501016:230:HFVJLAFXY:3:11410:5870:12176/67‑1 (MQ=49)
                                        ccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGACCGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCtgccagggc                                                                                                             >  NB501016:230:HFVJLAFXY:1:21204:1696:12889/45‑142 (MQ=60)
                                        ccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccaggg                                                                                                              <  NB501016:230:HFVJLAFXY:3:11608:3277:16892/106‑9 (MQ=60)
                                        ccaccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccagg                                                                                                               <  NB501016:230:HFVJLAFXY:4:21601:15130:7136/105‑8 (MQ=60)
                                         caccgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccagggcg                                                                                                            <  NB501016:230:HFVJLAFXY:3:21509:15448:19034/108‑11 (MQ=60)
                                          gtggcggcctctgacgctttcgtggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGG                                                                                                           >  NB501016:230:HFVJLAFXY:3:11601:21471:8383/43‑151 (MQ=60)
                                          accgcggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGG                                                                                                           <  NB501016:230:HFVJLAFXY:1:11303:14870:10312/109‑1 (MQ=60)
                                          ACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGGGGCGGCCTCAACAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGG                                                                                                           <  NB501016:230:HFVJLAFXY:3:21611:6857:2345/151‑1 (MQ=60)
                                              cggccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAA                                                                                                       <  NB501016:230:HFVJLAFXY:2:11208:15307:3266/113‑1 (MQ=60)
                                                gccacgacagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGGCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAA                                                                                                     <  NB501016:230:HFVJLAFXY:3:11501:15909:4169/115‑1 (MQ=60)
                                                 ccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAAC                                                                                                    >  NB501016:230:HFVJLAFXY:1:11105:4562:10886/36‑151 (MQ=60)
                                                 ccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAAC                                                                                                    <  NB501016:230:HFVJLAFXY:1:11202:7541:5986/116‑1 (MQ=60)
                                                 ccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAAC                                                                                                    <  NB501016:230:HFVJLAFXY:4:11603:22097:16192/116‑1 (MQ=60)
                                                 ccacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAAC                                                                                                    <  NB501016:230:HFVJLAFXY:1:21210:7463:12556/116‑1 (MQ=60)
                                                  cacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCTAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACG                                                                                                   <  NB501016:230:HFVJLAFXY:3:11509:23046:4829/117‑1 (MQ=60)
                                                  cacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACG                                                                                                   <  NB501016:230:HFVJLAFXY:4:11411:6966:18686/117‑1 (MQ=60)
                                                  cacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCA                                                                                                         <  NB501016:230:HFVJLAFXY:2:21203:4421:6176/111‑1 (MQ=60)
                                                  cacgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCGCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAACAACG                                                                                                   >  NB501016:230:HFVJLAFXY:3:11408:26553:13700/35‑151 (MQ=60)
                                                    cgaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTC                                                                                                 <  NB501016:230:HFVJLAFXY:1:11211:21222:3811/119‑1 (MQ=60)
                                                     gaaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCC                                                                                                >  NB501016:230:HFVJLAFXY:3:11510:19572:14371/32‑151 (MQ=60)
                                                      aaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCG                                                                                               <  NB501016:230:HFVJLAFXY:4:21504:16939:2384/121‑1 (MQ=60)
                                                      aaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCG                                                                                               <  NB501016:230:HFVJLAFXY:3:21504:10092:8900/121‑1 (MQ=60)
                                                      aaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCG                                                                                               <  NB501016:230:HFVJLAFXY:3:21601:6496:15987/121‑1 (MQ=60)
                                                      aaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCG                                                                                               <  NB501016:230:HFVJLAFXY:4:11511:20223:2429/121‑1 (MQ=60)
                                                      aaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCG                                                                                               <  NB501016:230:HFVJLAFXY:1:11102:22238:19691/121‑1 (MQ=60)
                                                      aaagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAA                                                                                                     >  NB501016:230:HFVJLAFXY:2:21201:18453:1525/31‑145 (MQ=60)
                                                      AAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCG                                                                                               >  NB501016:230:HFVJLAFXY:3:21511:20099:7640/1‑151 (MQ=60)
                                                       aagcgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGA                                                                                              <  NB501016:230:HFVJLAFXY:4:21406:20700:5004/122‑1 (MQ=60)
                                                         gcgtcagaggccgccacttcagcacgtGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCATCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGA                                                                                            <  NB501016:230:HFVJLAFXY:2:21311:10298:1409/124‑1 (MQ=60)
                                                          cgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGAC                                                                                           >  NB501016:230:HFVJLAFXY:1:21108:5560:1742/27‑151 (MQ=60)
                                                          cgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGAC                                                                                           >  NB501016:230:HFVJLAFXY:3:21406:9864:5492/27‑151 (MQ=60)
                                                          cgtcagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGAAGCAAAATCATCAGAAACGAACTCATCATCAAGTGCCGGTCGTGCAGCTTCATCGGCAACGGCGGCAGAAAATTCTGCCAGGCCGGCAAAAAAGACCGAGAC                                                                                           >  NB501016:230:HFVJLAFXY:3:11402:4017:17301/27‑151 (MQ=40)
                                                             cagaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAA                                                                                        <  NB501016:230:HFVJLAFXY:4:11608:9605:9915/128‑1 (MQ=60)
                                                               gaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATG                                                                                      <  NB501016:230:HFVJLAFXY:4:11405:2281:12184/130‑1 (MQ=60)
                                                               gaggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATG                                                                                      <  NB501016:230:HFVJLAFXY:2:11210:10239:6183/130‑1 (MQ=60)
                                                               gaggccgccacttcagcacgaGATGCGGTGGCCGCAAAAGACGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATG                                                                                      <  NB501016:230:HFVJLAFXY:2:21106:13036:8115/130‑1 (MQ=60)
                                                                 ggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCC                                                                                    <  NB501016:230:HFVJLAFXY:1:11308:21162:5961/132‑1 (MQ=60)
                                                                 ggccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCAGAGACGAATGCC                                                                                    >  NB501016:230:HFVJLAFXY:4:21403:9135:7583/20‑151 (MQ=60)
                                                                   ccgccacttcagcaggaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCATCGGCGGCTGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAG                                                                                  <  NB501016:230:HFVJLAFXY:1:21205:17105:12756/134‑1 (MQ=60)
                                                                   ccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAG                                                                                  <  NB501016:230:HFVJLAFXY:4:21405:11627:9939/134‑1 (MQ=60)
                                                                   ccgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCC                                                                                    >  NB501016:230:HFVJLAFXY:2:21302:20259:5509/18‑149 (MQ=60)
                                                                    cgccacttcagcacgaGATGCGGTGGCCTCAAAAGATGCAGCAAAATAATCATAAACTAACGCATCATCAAGTGCCGGGCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGG                                                                                 <  NB501016:230:HFVJLAFXY:3:21408:25002:17560/135‑1 (MQ=58)
                                                                    cgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGTCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGG                                                                                 <  NB501016:230:HFVJLAFXY:3:21403:10215:4875/135‑1 (MQ=60)
                                                                    cgccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGG                                                                                 <  NB501016:230:HFVJLAFXY:1:21310:8125:5008/135‑1 (MQ=60)
                                                                     gccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGT                                                                                <  NB501016:230:HFVJLAFXY:3:21603:20969:8722/136‑1 (MQ=60)
                                                                     gccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCAAGACGAATGCCAGGT                                                                                >  NB501016:230:HFVJLAFXY:3:11607:18592:14395/16‑151 (MQ=60)
                                                                     gccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGC‑AAAACGTCCGAGACGAATGCCAGGTC                                                                               >  NB501016:230:HFVJLAFXY:4:21410:4041:4716/16‑151 (MQ=60)
                                                                      ccacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTC                                                                               <  NB501016:230:HFVJLAFXY:3:11411:7995:10198/137‑1 (MQ=60)
                                                                       cacttcagcacgagatgcggtggcctcaaaagcgtcaGCAAAATCATCAGAACCGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGCGCGGCAAAAACGTCCGAGCCGAATGCAAGGTC                                                                               >  NB501016:230:HFVJLAFXY:2:21209:3467:12747/38‑150 (MQ=55)
                                                                       cacttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGG                                                                                                           >  NB501016:230:HFVJLAFXY:1:21304:21323:15944/14‑122 (MQ=60)
                                                                         cttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAAGGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGT                                                                                <  NB501016:230:HFVJLAFXY:4:11506:8342:6004/136‑1 (MQ=60)
                                                                         cttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGCACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATC                                                                            >  NB501016:230:HFVJLAFXY:2:21309:18272:12184/12‑151 (MQ=60)
                                                                         cttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAATTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATC                                                                            >  NB501016:230:HFVJLAFXY:1:21105:2755:2835/12‑151 (MQ=60)
                                                                          ttcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCT                                                                           >  NB501016:230:HFVJLAFXY:1:11301:6181:2260/11‑151 (MQ=60)
                                                                           tcagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCT                                                                           <  NB501016:230:HFVJLAFXY:2:11212:8374:8072/141‑1 (MQ=60)
                                                                            cagcactaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTG                                                                          <  NB501016:230:HFVJLAFXY:4:21507:2516:6926/142‑1 (MQ=60)
                                                                            cagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGACCGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGGCATCTG                                                                          <  NB501016:230:HFVJLAFXY:1:21306:24915:13878/142‑1 (MQ=60)
                                                                            cagcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTG                                                                          <  NB501016:230:HFVJLAFXY:4:11409:6455:16116/142‑1 (MQ=60)
                                                                             agcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAA                                                                        <  NB501016:230:HFVJLAFXY:4:21502:18868:9676/144‑1 (MQ=60)
                                                                             agcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAA                                                                        <  NB501016:230:HFVJLAFXY:1:21102:2421:3123/144‑1 (MQ=60)
                                                                             agcacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAA                                                                        <  NB501016:230:HFVJLAFXY:2:11312:20721:9471/144‑1 (MQ=60)
                                                                              GCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGCGACGAATGCCAGGTCATCTGAA                                                                        >  NB501016:230:HFVJLAFXY:2:21204:8533:3050/1‑150 (MQ=60)
                                                                              GCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAA                                                                        >  NB501016:230:HFVJLAFXY:2:11106:6392:7794/1‑150 (MQ=60)
                                                                              GCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTC                                                                                                                      >  NB501016:230:HFVJLAFXY:1:11208:10519:16191/1‑104 (MQ=60)
                                                                               cacgcGATGCGGTGGCCTCAAAAGAGGCATCAAAATCATCAGAAACGAACGCATCATCACGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGTGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAAC                                                                      <  NB501016:230:HFVJLAFXY:1:11112:14121:18358/146‑1 (MQ=60)
                                                                               cacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAAC                                                                      <  NB501016:230:HFVJLAFXY:2:11107:24559:11870/146‑1 (MQ=60)
                                                                               cacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAAC                                                                      >  NB501016:230:HFVJLAFXY:4:11403:16546:16283/6‑151 (MQ=60)
                                                                               cacgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAAC                                                                      >  NB501016:230:HFVJLAFXY:3:21608:22627:8616/6‑151 (MQ=60)
                                                                                acgaGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAG                                                                                  <  NB501016:230:HFVJLAFXY:4:21409:19508:19930/134‑1 (MQ=60)
                                                                                 CGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAG                                                                    <  NB501016:230:HFVJLAFXY:1:21212:23429:4942/151‑1 (MQ=60)
                                                                                 CGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAAC                                                                      >  NB501016:230:HFVJLAFXY:2:21207:5453:10489/1‑149 (MQ=60)
                                                                                    GATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAG                                                                 <  NB501016:230:HFVJLAFXY:1:11310:11430:6031/151‑1 (MQ=60)
                                                                                    GATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGC                                                                   >  NB501016:230:HFVJLAFXY:2:11305:8979:18782/1‑149 (MQ=60)
                                                                                      TGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGaaaatagatcggaagagcacacgtctgaactcagtcaccggctatgagctcgtatgccgtct                                                               >  NB501016:230:HFVJLAFXY:2:21206:1561:8049/1‑89 (MQ=60)
                                                                                      TGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCG                                                               >  NB501016:230:HFVJLAFXY:3:21506:14366:3162/1‑151 (MQ=60)
                                                                                       GCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCG                                                               <  NB501016:230:HFVJLAFXY:4:21407:23284:11484/150‑1 (MQ=60)
                                                                                         GGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAA                                                            <  NB501016:230:HFVJLAFXY:4:11503:13821:17167/151‑1 (MQ=60)
                                                                                         GGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGA                                                             >  NB501016:230:HFVJLAFXY:4:21611:14855:10257/1‑150 (MQ=60)
                                                                                            GGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGaacgg                                                         <  NB501016:230:HFVJLAFXY:2:21108:15910:15994/151‑6 (MQ=60)
                                                                                            GGCCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGaacgg                                                         <  NB501016:230:HFVJLAFXY:2:11110:2580:14552/151‑6 (MQ=60)
                                                                                              CCTCAAAAGAGGCAGCAAAATCATCAGAACCGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAG                                                       <  NB501016:230:HFVJLAFXY:3:11404:18109:13227/151‑1 (MQ=60)
                                                                                              CCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgcc                                                                                                                  >  NB501016:230:HFVJLAFXY:3:21404:3520:17533/1‑88 (MQ=60)
                                                                                              CCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGaacgg                                                         >  NB501016:230:HFVJLAFXY:1:11201:17782:1813/1‑144 (MQ=60)
                                                                                              CCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGaacgg                                                         >  NB501016:230:HFVJLAFXY:1:11212:20612:7248/1‑144 (MQ=60)
                                                                                              CCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGaacgg                                                         >  NB501016:230:HFVJLAFXY:3:21512:6069:7469/1‑144 (MQ=60)
                                                                                              CCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGaacgg                                                         >  NB501016:230:HFVJLAFXY:3:11604:8361:1775/1‑144 (MQ=60)
                                                                                              CCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAG                                                       <  NB501016:230:HFVJLAFXY:1:21103:19715:17708/151‑1 (MQ=60)
                                                                                              CCTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAG                                                                                             <  NB501016:230:HFVJLAFXY:4:11409:11170:13952/113‑1 (MQ=60)
                                                                                               CTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCtgccaggg                                                                                                              >  NB501016:230:HFVJLAFXY:2:11210:4770:9604/1‑87 (MQ=60)
                                                                                               CTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGCCGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGC                                                      >  NB501016:230:HFVJLAFXY:3:21502:10329:9266/1‑151 (MQ=60)
                                                                                               CTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGC                                                      >  NB501016:230:HFVJLAFXY:2:21308:15134:16034/1‑151 (MQ=60)
                                                                                               CTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGC                                                      >  NB501016:230:HFVJLAFXY:2:21307:1057:8165/1‑151 (MQ=60)
                                                                                               CTCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCA                                                                                   <  NB501016:230:HFVJLAFXY:3:11606:17976:7807/122‑1 (MQ=60)
                                                                                                TCAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGACGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCG                                                     >  NB501016:230:HFVJLAFXY:4:11509:14217:18661/1‑151 (MQ=60)
                                                                                                 CATAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCG                                                     <  NB501016:230:HFVJLAFXY:1:11204:17036:2710/150‑1 (MQ=60)
                                                                                                 CAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGC                                                    >  NB501016:230:HFVJLAFXY:4:11501:10521:15370/1‑151 (MQ=60)
                                                                                                 CAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGC                                                    >  NB501016:230:HFVJLAFXY:2:21305:20011:5506/1‑151 (MQ=60)
                                                                                                 CAAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCATGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGA                                                                         >  NB501016:230:HFVJLAFXY:1:21302:21696:5652/1‑130 (MQ=60)
                                                                                                  AAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCC                                                   >  NB501016:230:HFVJLAFXY:4:21609:2044:11659/1‑151 (MQ=60)
                                                                                                  AAAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTG                                                                                                                    >  NB501016:230:HFVJLAFXY:4:11407:10476:4340/1‑86 (MQ=60)
                                                                                                   AAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCT                                                  <  NB501016:230:HFVJLAFXY:3:21606:5924:6589/151‑1 (MQ=60)
                                                                                                   AAAGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCCGTCGTGCCGCTTCCTCTGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCC                                                   >  NB501016:230:HFVJLAFXY:1:11109:12295:7693/1‑150 (MQ=60)
                                                                                                     AGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGATACTAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCT                                                <  NB501016:230:HFVJLAFXY:2:11311:17824:3288/151‑1 (MQ=60)
                                                                                                     AGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCT                                                <  NB501016:230:HFVJLAFXY:1:21203:18750:10682/151‑1 (MQ=60)
                                                                                                     AGAGGCAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCT                                                <  NB501016:230:HFVJLAFXY:4:21603:20978:15869/151‑1 (MQ=60)
                                                                                                        ggcaGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCTGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATATGAAACAGCAGCGGaacggatcgcctctgcc                                             >  NB501016:230:HFVJLAFXY:4:21604:24673:5685/5‑134 (MQ=60)
                                                                                                         GCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTG                                            <  NB501016:230:HFVJLAFXY:2:11304:13887:19273/151‑1 (MQ=60)
                                                                                                          CAGCAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGC                                           >  NB501016:230:HFVJLAFXY:4:21508:21765:7864/1‑151 (MQ=60)
                                                                                                             CAAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGC                                        <  NB501016:230:HFVJLAFXY:2:21111:8313:19430/151‑1 (MQ=60)
                                                                                                              AAAATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCA                                       <  NB501016:230:HFVJLAFXY:1:21206:7185:6967/151‑1 (MQ=60)
                                                                                                                 ATCATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGAC                                    <  NB501016:230:HFVJLAFXY:3:21510:10425:11615/151‑1 (MQ=60)
                                                                                                                    ATCAGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGacgc                                  >  NB501016:230:HFVJLAFXY:1:11103:6242:1099/1‑146 (MQ=60)
                                                                                                                       AGAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAA                               >  NB501016:230:HFVJLAFXY:4:11411:11113:18264/1‑150 (MQ=60)
                                                                                                                        GAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAA                              >  NB501016:230:HFVJLAFXY:1:11101:10916:18123/1‑150 (MQ=60)
                                                                                                                        GAAACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAA                              >  NB501016:230:HFVJLAFXY:2:11308:14977:19668/1‑150 (MQ=60)
                                                                                                                         AAACGAACGCATCATCAAGTGCCGGACGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAA                             >  NB501016:230:HFVJLAFXY:2:21109:8984:16301/1‑150 (MQ=60)
                                                                                                                          AACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGacgcaaacacc                           >  NB501016:230:HFVJLAFXY:3:11401:23018:4174/1‑140 (MQ=60)
                                                                                                                          AACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAAC                            >  NB501016:230:HFVJLAFXY:1:21303:4584:7342/1‑150 (MQ=60)
                                                                                                                          AACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTC                                                 <  NB501016:230:HFVJLAFXY:3:21406:13603:14761/129‑1 (MQ=60)
                                                                                                                           ACGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAG                          <  NB501016:230:HFVJLAFXY:4:21607:18820:8859/151‑1 (MQ=60)
                                                                                                                            CGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGC                         >  NB501016:230:HFVJLAFXY:2:11204:2933:13652/1‑151 (MQ=60)
                                                                                                                              AACGCATCATCAAGAGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGG                       <  NB501016:230:HFVJLAFXY:3:11507:21608:17917/151‑1 (MQ=60)
                                                                                                                               ACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGGCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGC                      >  NB501016:230:HFVJLAFXY:1:21305:25891:19078/1‑151 (MQ=60)
                                                                                                                                CGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGCATGCCAGGTCATCTGACACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGCCGCAAAAACAGCGGCG                     >  NB501016:230:HFVJLAFXY:1:21209:24790:8685/1‑151 (MQ=53)
                                                                                                                                 GCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGG                       <  NB501016:230:HFVJLAFXY:1:21208:18262:19253/148‑1 (MQ=60)
                                                                                                                                  CATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGCCGCAAAAACAGCGGCGGC                   >  NB501016:230:HFVJLAFXY:4:21602:20402:7861/1‑151 (MQ=60)
                                                                                                                                  CATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCGGC                   >  NB501016:230:HFVJLAFXY:2:11303:13119:18880/1‑151 (MQ=60)
                                                                                                                                  CATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGC                                                                   <  NB501016:230:HFVJLAFXY:1:11206:25269:17744/103‑1 (MQ=60)
                                                                                                                                   ATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTatgccagggcggcaaagccggcagagccgcatgcccggtcctctgaaacagcagcggcccggagcgcctctgccgcggcagacgaaaaaacagcggcggcg                  >  NB501016:230:HFVJLAFXY:4:21501:12253:5007/1‑50 (MQ=28)
                                                                                                                                   ATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGC                         <  NB501016:230:HFVJLAFXY:1:21202:17431:1587/144‑1 (MQ=60)
                                                                                                                                     CATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGTAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggg                >  NB501016:230:HFVJLAFXY:4:11604:23040:14040/1‑145 (MQ=54)
                                                                                                                                     CATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGCACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggg                >  NB501016:230:HFVJLAFXY:3:11610:3154:18264/1‑145 (MQ=60)
                                                                                                                                     CATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcgg                 <  NB501016:230:HFVJLAFXY:1:21112:9150:12187/150‑6 (MQ=60)
                                                                                                                                     CATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACCGCGGCAGAAAATTCTGCCACGGCGGCAAAAACGTCCGAGACGAAAGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcgcg                >  NB501016:230:HFVJLAFXY:3:11503:19487:12658/1‑145 (MQ=54)
                                                                                                                                      ATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGCACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcgggg               >  NB501016:230:HFVJLAFXY:3:11407:25371:6842/1‑144 (MQ=60)
                                                                                                                                      ATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcgggg               >  NB501016:230:HFVJLAFXY:2:21103:12479:2741/1‑144 (MQ=60)
                                                                                                                                      ATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcgggg               >  NB501016:230:HFVJLAFXY:3:11508:2950:14205/1‑144 (MQ=60)
                                                                                                                                      ATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcgggg               >  NB501016:230:HFVJLAFXY:1:21110:14562:6789/1‑144 (MQ=60)
                                                                                                                                      ATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcgggg               >  NB501016:230:HFVJLAFXY:1:21104:8072:18206/1‑144 (MQ=60)
                                                                                                                                      ATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcgggg               <  NB501016:230:HFVJLAFXY:1:11305:2851:3565/151‑8 (MQ=60)
                                                                                                                                      ATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGGCCGCGACGAAGGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcgggg               <  NB501016:230:HFVJLAFXY:3:21408:12913:10229/151‑8 (MQ=47)
                                                                                                                                        CAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggag             <  NB501016:230:HFVJLAFXY:4:21511:11195:6962/151‑10 (MQ=60)
                                                                                                                                        CAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggag             <  NB501016:230:HFVJLAFXY:2:11102:22664:12964/151‑10 (MQ=60)
                                                                                                                                        CAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggag             <  NB501016:230:HFVJLAFXY:4:11611:19880:14633/151‑10 (MQ=60)
                                                                                                                                         AAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggagt            >  NB501016:230:HFVJLAFXY:1:11207:12121:7596/1‑141 (MQ=60)
                                                                                                                                         AAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGG                                                                                 >  NB501016:230:HFVJLAFXY:1:11312:8649:7872/1‑82 (MQ=52)
                                                                                                                                             GCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggagtgcgt        >  NB501016:230:HFVJLAFXY:3:21610:17623:6440/1‑137 (MQ=60)
                                                                                                                                             GCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggagtgcgt        <  NB501016:230:HFVJLAFXY:2:21301:4139:8520/151‑15 (MQ=60)
                                                                                                                                              CCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggagtgcgtc       <  NB501016:230:HFVJLAFXY:2:21103:9533:8895/151‑16 (MQ=60)
                                                                                                                                                   CGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggagtgcgtcaacgg  <  NB501016:230:HFVJLAFXY:4:11404:19136:20246/151‑21 (MQ=60)
                                                                                                                                                   CGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggagtgcgtcaacgg  <  NB501016:230:HFVJLAFXY:4:11601:2257:14051/151‑21 (MQ=60)
                                                                                                                                                   CGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGGCCGAGACGAATGCCAGGGCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggagtgcgtcaacg   <  NB501016:230:HFVJLAFXY:3:11511:2680:1946/150‑20 (MQ=50)
                                                                                                                                                   CGTGCAGCGTCCTCGGCAACGGCGGCAGAAAATTCTGCCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGAGCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCggcggggagtgcgtcaacgg  <  NB501016:230:HFVJLAFXY:4:21408:2066:1697/151‑21 (MQ=59)
                                                                                                                                                   |                                                                                                                                                      
AATGCTTCAGCGTCACTACAATCAGCAGCCACATCTGCATCCACCGCGACCACGAAGGCATCAGAAGCTGCGACCTCGGCCCGGGATGCGGCGGCCTCAAAAGAAGCGGCAAAATCATCAGAAACGAACGCATCATCAAGCGCCAGTAGTGCAGCTTCCTCGGCAACGGCGGCAGGAAATTCCGCGAAGGCGGCAAAAACGTCCGAGACGAACGCCAGGTCTTCTGAAACGGCAGCGGGACAGAGCGCCTCGGCTGCGGCAGGCTCAAAAACAGCGGCTGCGTCGTCTGCCAGTGCAG  >  NC_000913/1429676‑1429973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑