Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,636,128 C→T 100% pseudogene (240/528 nt) nohQ ← pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,636,1280CT98.7% 5379.3 / 22.3 2653pseudogene (240/528 nt)nohQpseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin
Reads supporting (aligned to +/- strand):  ref base C (22/16);  new base T (1382/1224);  total (1411/1242)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 2680 total aligned reads displayed.

AGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATCTTATAACGGCGGCAGAGTCATAAAGCACCTCATTACCCTTGCCAC  >  NC_000913/1635982‑1636270
                                                                                                                                                  |                                                                                                                                              
agaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCCGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTtcc                                                                                                                                              <  2:268921/149‑1 (MQ=255)
  aaTACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctg                                                                                                                                            <  4:155690/149‑1 (MQ=12)
   aTACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctgg                                                                                                                                           <  8:188173/149‑1 (MQ=12)
   aTACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctgg                                                                                                                                           <  3:138053/149‑1 (MQ=255)
       tgccAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCCCCACTTCAGCGGAGTATCTGGCAGTCTTCAGGTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGGTCGTACTCAATAGTGActgcctg                                                                                                                                        >  2:219197/4‑148 (MQ=11)
       tGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTAATCAATAGTTCctggctg                                                                                                                                        >  3:246190/1‑148 (MQ=255)
       tGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAAAGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctg                                                                                                                                        >  1:156657/1‑148 (MQ=255)
         gCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctgg                                                                                                                                       <  7:343342/147‑1 (MQ=12)
         gCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctg                                                                                                                                        >  4:68265/1‑146 (MQ=12)
         gCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGga                                                                                                                                      <  4:18965/148‑1 (MQ=12)
         gCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGga                                                                                                                                      <  6:202575/148‑1 (MQ=12)
           aaTTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctg                                                                                                                                        >  1:122339/1‑144 (MQ=12)
           aaTTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGAt                                                                                                                                   <  3:59733/149‑1 (MQ=12)
            aagtCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGTCATTCTTCAGTTCCTGTGCGTCTGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCTATAGTTCCTGGCTGGAGATc                                                                                                                                  <  4:42105/146‑1 (MQ=11)
            aTTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATc                                                                                                                                  <  6:154464/149‑1 (MQ=12)
                cACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTgc                                                                                                                               <  6:35669/148‑2 (MQ=12)
                 aCCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCGCTGGCAGTCTGCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGcct                                                                                                                             <  8:253442/149‑1 (MQ=11)
                  ccTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGcctc                                                                                                                            <  3:317252/149‑1 (MQ=255)
                   cTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctg                                                                                                                                        >  2:374640/1‑136 (MQ=12)
                    tGCGATCCGCGACAGCACCAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGc                                                                                                                          >  7:153642/1‑149 (MQ=11)
                     gCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCt                                                                                                                         >  5:333402/1‑149 (MQ=255)
                      cGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGc                                                                                                                          <  5:2747/147‑1 (MQ=255)
                      cGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTg                                                                                                                        >  1:79142/1‑149 (MQ=255)
                      cGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTg                                                                                                                        <  4:119092/149‑1 (MQ=255)
                      cGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTg                                                                                                                        <  6:277075/149‑1 (MQ=255)
                       gATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTgcc                                                                                                                              <  8:154175/142‑3 (MQ=12)
                       gATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGc                                                                                                                          <  2:104256/146‑1 (MQ=255)
                       gATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGGTCGTACTCAATAGTTCCTGGCGGGAGATCTGCCTCGCTgg                                                                                                                       >  2:131589/1‑149 (MQ=11)
                         tCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGTGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGcc                                                                                                                     <  6:224719/149‑1 (MQ=11)
                         tCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGcc                                                                                                                     >  5:360344/1‑149 (MQ=255)
                         tCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGACGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGcc                                                                                                                     >  2:43249/1‑149 (MQ=11)
                            gcgACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCt                                                                                                                                 >  8:339405/1‑134 (MQ=12)
                            gcgACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGc                                                                                                                  >  3:369541/1‑149 (MQ=255)
                             cgACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTgg                                                                                                                       <  3:342030/143‑1 (MQ=255)
                             cgACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTgg                                                                                                                       >  4:342030/1‑143 (MQ=255)
                                   cACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGtt                                                                                                           <  6:302848/149‑1 (MQ=255)
                                    aCGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttc                                                                                                          >  7:91659/1‑149 (MQ=255)
                                    aCGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttc                                                                                                          >  6:106561/1‑149 (MQ=255)
                                    aCGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGGTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttc                                                                                                          <  5:147789/149‑1 (MQ=11)
                                     cGAAAGTACAGAATGCGGTTTGCTCGACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGGGTCGGCGTGCGCTCGCGTTAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttct                                                                                                         <  3:168464/149‑1 (MQ=11)
                                        aaGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctgg                                                                                                                                       >  8:71344/1‑116 (MQ=255)
                                        aaGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttcttc                                                                                                       >  6:381188/1‑148 (MQ=255)
                                          gTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAAc                                                                                                    <  7:260301/149‑1 (MQ=255)
                                          gTACAGAATGCGGTTTCCACCACTTCAGCGGAGGCGCTGGCAGTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAAc                                                                                                    <  4:297951/149‑1 (MQ=11)
                                           tACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAAcc                                                                                                   >  6:248074/1‑148 (MQ=255)
                                              aGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTcc                                                                                                >  1:215901/1‑149 (MQ=11)
                                              aGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTcc                                                                                                >  3:292980/1‑149 (MQ=11)
                                              aGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTcc                                                                                                >  3:203084/1‑149 (MQ=11)
                                                      ggttCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAg                                                                                        <  1:293446/147‑1 (MQ=11)
                                                         tCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCtt                                                                                     >  5:224561/1‑149 (MQ=11)
                                                         tCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCATGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCt                                                                                      <  3:90146/148‑1 (MQ=11)
                                                           caccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCtttt                                                                                   >  8:11959/1‑149 (MQ=11)
                                                            accacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTc                                                                                  <  6:179376/149‑1 (MQ=11)
                                                            accacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTc                                                                                  <  8:222566/149‑1 (MQ=11)
                                                            accacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTc                                                                                  >  6:13113/1‑149 (MQ=11)
                                                             ccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCg                                                                                 <  1:100552/149‑1 (MQ=11)
                                                             ccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCg                                                                                 >  5:297476/1‑149 (MQ=11)
                                                              cacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGt                                                                                <  6:67403/149‑1 (MQ=11)
                                                               acTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGtt                                                                               <  2:345323/149‑1 (MQ=11)
                                                                cTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTtc                                                                              <  6:92040/149‑1 (MQ=11)
                                                                 ttCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttcttc                                                                                                       <  5:380988/123‑1 (MQ=255)
                                                                 ttCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTc                                                                                  >  2:166207/1‑144 (MQ=11)
                                                                 ttCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTtct                                                                             >  7:212138/1‑149 (MQ=11)
                                                                    aGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCaa                                                                          >  1:375593/1‑149 (MQ=37)
                                                                     gCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAAt                                                                         <  8:314132/149‑1 (MQ=11)
                                                                     gCGGAGTCTCTGGCATTCTTCAGTTACTGTGAGTCGGCCTGCGCACGCGTAAGTCGATCGCGTTCGTACTCAATAGTTCCTTGCTGTAGATCTGCCTAGCTGGCCTGCCGCAGTTCTACAACCTCCCGGCGAAGCTTTTCGTTAACAAt                                                                         >  1:49691/1‑149 (MQ=11)
                                                                       ggAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCt                                                                                                                    >  4:95684/1‑104 (MQ=11)
                                                                        gAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCACCCTCCCGGCGCAGCTTTTCGTTCTCAAttt                                                                       >  5:245885/1‑148 (MQ=11)
                                                                         aGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAGCCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa                                                                     <  3:10439/149‑1 (MQ=11)
                                                                         aGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa                                                                     <  2:185963/149‑1 (MQ=11)
                                                                         aGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa                                                                     <  8:279921/149‑1 (MQ=11)
                                                                         aGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa                                                                     <  7:65523/149‑1 (MQ=11)
                                                                         aGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa                                                                     <  5:70713/149‑1 (MQ=11)
                                                                           gctctTGCATTCTTCTGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCTATAGTTCCTGGCTGGAGTTCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGc                                                                   <  1:318274/148‑1 (MQ=11)
                                                                             tctGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTTAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAAc                                                                                                    <  7:120233/114‑1 (MQ=11)
                                                                               tGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATcc                                                               >  8:399623/1‑149 (MQ=11)
                                                                               tGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATcc                                                               >  5:201033/1‑149 (MQ=11)
                                                                               tGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATcc                                                               >  7:397901/1‑149 (MQ=11)
                                                                                ggCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATccc                                                              >  1:350108/1‑149 (MQ=11)
                                                                                 gCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCt                                                             >  1:264349/1‑149 (MQ=11)
                                                                                 gCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACCCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCaa                                                                          >  2:235917/1‑136 (MQ=11)
                                                                                 gCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACCCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCaa                                                                          <  1:235917/136‑1 (MQ=11)
                                                                                   aTTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCttt                                                           >  7:241477/1‑149 (MQ=11)
                                                                                    ttcttCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTTCTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTc                                                          >  5:120337/4‑149 (MQ=11)
                                                                                    ttcttCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATATGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTc                                                          >  3:269889/4‑149 (MQ=11)
                                                                                    ttcttCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGAGCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTc                                                          <  4:216474/146‑1 (MQ=11)
                                                                                    ttcttCAGTTACTGTGCGTCGGACTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTc                                                          >  2:327774/4‑149 (MQ=11)
                                                                                       ttCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttcttc                                                                                                       <  2:297990/101‑1 (MQ=255)
                                                                                       ttCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCgg                                                        >  6:334413/1‑148 (MQ=255)
                                                                                         cAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCgg                                                        <  5:174277/146‑1 (MQ=255)
                                                                                         cAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGc                                                       <  1:185143/147‑1 (MQ=255)
                                                                                         cAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGACGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCgg                                                        <  7:369760/146‑1 (MQ=11)
                                                                                          agttCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTGCGTACTCAATAGTTCCTGGCTGGAGAGCTGCCTCGCTGGCCGGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATa                                                    <  4:276263/146‑1 (MQ=11)
                                                                                           gttCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATAc                                                   >  2:77600/3‑149 (MQ=12)
                                                                                            ttCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATAc                                                   >  7:318284/2‑148 (MQ=12)
                                                                                             tCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCa                                                 >  8:366031/1‑149 (MQ=12)
                                                                                              ccTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCAt                                                >  7:179425/1‑149 (MQ=12)
                                                                                                tgtgCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa                                                                     <  7:296450/126‑1 (MQ=12)
                                                                                                tgtgCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCGGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTc                                                          <  4:24552/137‑1 (MQ=255)
                                                                                                    cGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttc                                                                                                          <  4:317884/85‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
AGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATCTTATAACGGCGGCAGAGTCATAAAGCACCTCATTACCCTTGCCAC  >  NC_000913/1635982‑1636270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 1443 total aligned reads displayed.
GTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTT‑TTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATCTTATAACGGCGGCAGAGTCATAAAGCACCTCATTACCCTTGCCACC  >  NC_000913/1635978‑1636271
                                                                                                                                                       |                                                                                                                                               
GTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCTGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTT                                                                                                                                                 <  NB501016:230:HFVJLAFXY:1:11302:8481:5222/151‑1 (MQ=7)
  CGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCC                                                                                                                                               >  NB501016:230:HFVJLAFXY:3:11511:15332:13630/1‑151 (MQ=7)
  CGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCC                                                                                                                                               >  NB501016:230:HFVJLAFXY:4:11405:13233:9627/1‑151 (MQ=7)
   GAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCT                                                                                                                                              >  NB501016:230:HFVJLAFXY:2:21203:18985:1399/1‑151 (MQ=7)
   GAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCT                                                                                                                                              >  NB501016:230:HFVJLAFXY:2:21307:13957:2347/1‑151 (MQ=7)
    AGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTG                                                                                                                                             <  NB501016:230:HFVJLAFXY:3:21503:2791:12634/151‑1 (MQ=7)
     GAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGG                                                                                                                                            <  NB501016:230:HFVJLAFXY:4:21610:1617:17650/151‑1 (MQ=7)
     GAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGG                                                                                                                                            <  NB501016:230:HFVJLAFXY:1:21108:8277:13509/151‑1 (MQ=7)
      AATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGC                                                                                                                                           <  NB501016:230:HFVJLAFXY:4:11506:9703:15882/151‑1 (MQ=7)
        TACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTG                                                                                                                                         >  NB501016:230:HFVJLAFXY:2:21102:15888:4805/1‑151 (MQ=7)
         ACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGACTGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:2:11104:17243:1552/151‑1 (MQ=7)
         ACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:3:21604:18470:18522/151‑1 (MQ=7)
         ACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTG                                                                                                                                         <  NB501016:230:HFVJLAFXY:1:11204:7680:13475/150‑1 (MQ=7)
          CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:2:11102:5074:17551/1‑150 (MQ=7)
          CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:2:11211:11818:3600/1‑150 (MQ=7)
          CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:1:21110:3487:7677/1‑150 (MQ=7)
          CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:4:11507:4584:8173/1‑150 (MQ=7)
          CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:4:11604:24908:17109/1‑150 (MQ=7)
          CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTG                                                                                                                                             >  NB501016:230:HFVJLAFXY:1:11109:3200:19435/1‑145 (MQ=7)
          CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTAATCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:2:21201:21958:1500/1‑150 (MQ=7)
          CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGGAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGG                                                                                                                                       >  NB501016:230:HFVJLAFXY:1:11110:19100:15007/1‑151 (MQ=7)
          CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTAGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:2:11206:8701:2237/1‑150 (MQ=7)
            GGCATTTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCAGCACTTCAGCGGAGTCTCTGGAATTC‑TTCAGTTCCTGTGCGTCGGGCTGCGCAGGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAG                                                                                                                                      <  NB501016:230:HFVJLAFXY:2:11305:24922:4279/150‑1 (MQ=6)
            GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGC                                                                                                                                     >  NB501016:230:HFVJLAFXY:3:21406:6173:12957/1‑151 (MQ=7)
            GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:2:11302:9905:8417/1‑148 (MQ=7)
            GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:1:21104:5697:12505/1‑148 (MQ=7)
            GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:4:21604:14796:6528/1‑148 (MQ=7)
            GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCT                                                                                                                                          <  NB501016:230:HFVJLAFXY:3:21507:21937:10754/146‑1 (MQ=7)
            GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCCGGCTGGAG                                                                                                                                      <  NB501016:230:HFVJLAFXY:3:21510:7365:4778/150‑1 (MQ=6)
            GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTATCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:3:21605:24841:6051/1‑148 (MQ=7)
              CAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATC                                                                                                                                   <  NB501016:230:HFVJLAFXY:3:21610:15254:6754/151‑1 (MQ=7)
               ATTTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCT                                                                                                                                  <  NB501016:230:HFVJLAFXY:4:21402:4388:13228/151‑1 (MQ=7)
               AATTTCACCTGCGATCCGCGACCGCACGAAAGTACAGAATGCGGTTTCCGCCACTTCAGCGGAGTCTCTGGCATGC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCT                                                                                                                                  <  NB501016:230:HFVJLAFXY:2:21107:19782:7462/151‑1 (MQ=6)
               AATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCT                                                                                                                                  <  NB501016:230:HFVJLAFXY:4:11401:4520:10435/151‑1 (MQ=7)
                ATTTCACCTGCGATCCGCGAGAGCACGATAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACTCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                                                                                 <  NB501016:230:HFVJLAFXY:4:21608:13996:17870/151‑1 (MQ=6)
                ATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11504:13469:18104/1‑151 (MQ=7)
                ATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                                                                                 >  NB501016:230:HFVJLAFXY:3:11601:23298:4878/1‑151 (MQ=7)
                ATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11411:13867:7222/1‑151 (MQ=7)
                  TTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCTTACTCAATAGTTCCTGGCTGGAGATCTGCC                                                                                                                               >  NB501016:230:HFVJLAFXY:1:21310:2718:15507/1‑151 (MQ=7)
                  TTCACCTGCGAGCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGC                                                                                                                                <  NB501016:230:HFVJLAFXY:1:21208:4294:5029/150‑1 (MQ=7)
                   TCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCC                                                                                                                               <  NB501016:230:HFVJLAFXY:4:21407:3204:9152/150‑1 (MQ=7)
                    CACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTC                                                                                                                             >  NB501016:230:HFVJLAFXY:1:21207:7039:6478/1‑151 (MQ=7)
                    CACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTC                                                                                                                             >  NB501016:230:HFVJLAFXY:3:21405:12232:3881/1‑151 (MQ=7)
                    CACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:3:21406:10051:13278/1‑140 (MQ=7)
                     ACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCAAGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTCGCTGGAGATCTGccacg                                                                                                                            >  NB501016:230:HFVJLAFXY:1:11210:15241:20093/1‑146 (MQ=6)
                     ACCTGCGAGCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCG                                                                                                                            <  NB501016:230:HFVJLAFXY:3:21403:14121:13554/151‑1 (MQ=6)
                      CCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGC                                                                                                                           >  NB501016:230:HFVJLAFXY:3:11609:7642:15811/1‑151 (MQ=7)
                      CCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGC                                                                                                                           >  NB501016:230:HFVJLAFXY:2:11107:1538:15783/1‑151 (MQ=7)
                      CCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                                                                                 <  NB501016:230:HFVJLAFXY:3:11512:11546:14293/145‑1 (MQ=7)
                       CTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCT                                                                                                                          >  NB501016:230:HFVJLAFXY:4:11412:15434:3344/1‑151 (MQ=7)
                       CTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCT                                                                                                                          >  NB501016:230:HFVJLAFXY:4:21607:22063:7829/1‑151 (MQ=7)
                        TGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCCCGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTG                                                                                                                         >  NB501016:230:HFVJLAFXY:3:21611:1434:19101/1‑151 (MQ=6)
                         GCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGG                                                                                                                        <  NB501016:230:HFVJLAFXY:4:21611:15321:15078/151‑1 (MQ=7)
                         GCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCT                                                                                                                          <  NB501016:230:HFVJLAFXY:4:11509:4139:16884/149‑1 (MQ=7)
                          CGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGC                                                                                                                       <  NB501016:230:HFVJLAFXY:2:11207:9842:14759/151‑1 (MQ=7)
                           GATCCGCGACCGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATC                                                                                                                                   <  NB501016:230:HFVJLAFXY:3:11501:11449:18365/138‑1 (MQ=7)
                           GATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCC                                                                                                                      <  NB501016:230:HFVJLAFXY:2:21108:4402:2672/151‑1 (MQ=7)
                           GATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCC                                                                                                                      >  NB501016:230:HFVJLAFXY:3:21408:11166:13262/1‑151 (MQ=7)
                           GATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCC                                                                                                                      >  NB501016:230:HFVJLAFXY:2:11112:13842:16481/1‑151 (MQ=7)
                            ATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCT                                                                                                                     >  NB501016:230:HFVJLAFXY:2:11312:6854:17742/1‑151 (MQ=7)
                            ATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGACATCTGCCTCGCTGGCC                                                                                                                      >  NB501016:230:HFVJLAFXY:2:11101:13204:10488/1‑150 (MQ=6)
                            ATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGC                                                                                                                                           >  NB501016:230:HFVJLAFXY:2:21208:13120:8873/1‑129 (MQ=7)
                              CCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGC                                                                                                                   >  NB501016:230:HFVJLAFXY:4:21412:2669:14311/1‑151 (MQ=7)
                               CGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCC                                                                                                                  >  NB501016:230:HFVJLAFXY:2:21304:24036:9518/1‑151 (MQ=9)
                               CGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCT                                                                                                                                              <  NB501016:230:HFVJLAFXY:1:21103:1535:15560/123‑1 (MQ=8)
                               CGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACATCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCC                                                                                                                  >  NB501016:230:HFVJLAFXY:2:21209:12328:9662/1‑151 (MQ=8)
                                GCGACAGCACGAAAGTACAGAATGCGGTTTGCACCACTTCAGCGGAGTCTCTGGCATGC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTCCTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCG                                                                                                                 <  NB501016:230:HFVJLAFXY:3:21504:24288:3307/151‑1 (MQ=9)
                                GCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCG                                                                                                                 <  NB501016:230:HFVJLAFXY:1:21102:12573:14490/151‑1 (MQ=11)
                                GCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCG                                                                                                                 <  NB501016:230:HFVJLAFXY:3:21609:21473:12817/151‑1 (MQ=11)
                                 CGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGC                                                                                                                <  NB501016:230:HFVJLAFXY:2:21302:14541:4509/151‑1 (MQ=15)
                                 CGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGC                                                                                                                <  NB501016:230:HFVJLAFXY:3:11404:21248:19583/151‑1 (MQ=15)
                                  GACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCA                                                                                                               <  NB501016:230:HFVJLAFXY:1:11107:2618:18469/151‑1 (MQ=17)
                                  GACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGC                                                                                                                >  NB501016:230:HFVJLAFXY:4:11610:15444:5444/1‑150 (MQ=15)
                                     AGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTT                                                                                                            <  NB501016:230:HFVJLAFXY:4:11502:20143:10141/151‑1 (MQ=19)
                                      GCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTC                                                                                                           >  NB501016:230:HFVJLAFXY:4:21508:12548:1551/1‑151 (MQ=19)
                                      GCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTT                                                                                                            >  NB501016:230:HFVJLAFXY:4:21606:14550:13557/1‑150 (MQ=19)
                                      GCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCGCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTCCTCAATAGTTCCTGGCTGGAGCTCTGCCTCGCTGGCCTGCCGCAGTTC                                                                                                           >  NB501016:230:HFVJLAFXY:4:21512:8382:15547/1‑151 (MQ=18)
                                        ACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTT                                                                                                         <  NB501016:230:HFVJLAFXY:4:21409:11565:16094/151‑1 (MQ=19)
                                         CGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCCCGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCCGTTCTTC                                                                                                        >  NB501016:230:HFVJLAFXY:3:11409:11696:18839/1‑151 (MQ=18)
                                         CGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATTTGCCTCGCTGGCCTGCCGCAGTTCTTC                                                                                                        >  NB501016:230:HFVJLAFXY:3:11402:19782:13615/1‑151 (MQ=19)
                                         CGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTC                                                                                                        <  NB501016:230:HFVJLAFXY:1:11202:4257:6181/151‑1 (MQ=19)
                                         CGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTC                                                                                                        >  NB501016:230:HFVJLAFXY:1:21204:14567:12874/1‑151 (MQ=19)
                                          GAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCA                                                                                                       <  NB501016:230:HFVJLAFXY:2:21306:13705:8542/151‑1 (MQ=19)
                                          GAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCA                                                                                                       <  NB501016:230:HFVJLAFXY:3:21410:3474:11093/151‑1 (MQ=19)
                                          GAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCA                                                                                                       <  NB501016:230:HFVJLAFXY:3:11604:22273:13212/151‑1 (MQ=19)
                                          GAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTC                                                                                                        >  NB501016:230:HFVJLAFXY:3:11508:15272:20181/1‑150 (MQ=19)
                                            AAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAAC                                                                                                     <  NB501016:230:HFVJLAFXY:1:11309:13738:1480/151‑1 (MQ=19)
                                             gtaatacgcatcatcactggCCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACC                                                                                                    <  NB501016:230:HFVJLAFXY:1:21112:10483:15195/131‑1 (MQ=18)
                                             AGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACC                                                                                                    <  NB501016:230:HFVJLAFXY:4:11505:7481:4333/151‑1 (MQ=17)
                                             AGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACC                                                                                                    >  NB501016:230:HFVJLAFXY:1:11307:22831:17586/1‑151 (MQ=17)
                                              GTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCT                                                                                                   >  NB501016:230:HFVJLAFXY:2:21311:19781:13300/1‑151 (MQ=15)
                                                ACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTACGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATATGCATCGCTGCCCTGCCGCAGTTCTTCACCCTCC                                                                                                 >  NB501016:230:HFVJLAFXY:2:11209:19422:13656/1‑151 (MQ=15)
                                                 CAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCC                                                                                                >  NB501016:230:HFVJLAFXY:4:11406:6875:20284/1‑151 (MQ=6)
                                                  AGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCG                                                                                               >  NB501016:230:HFVJLAFXY:1:21212:10391:11161/1‑151 (MQ=6)
                                                  AGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGC                                                                                                                           >  NB501016:230:HFVJLAFXY:3:21611:9306:10523/1‑123 (MQ=7)
                                                   GAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGG                                                                                              >  NB501016:230:HFVJLAFXY:4:21410:22374:10276/1‑151 (MQ=6)
                                                   GAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGG                                                                                              >  NB501016:230:HFVJLAFXY:4:21507:19004:8543/1‑151 (MQ=6)
                                                     ATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCGGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCG                                                                                            >  NB501016:230:HFVJLAFXY:2:21105:25488:6537/1‑151 (MQ=6)
                                                     ATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCG                                                                                            >  NB501016:230:HFVJLAFXY:1:11104:1993:12132/1‑151 (MQ=6)
                                                     ATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGATCCTGTGCGTCGGCCAGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCG                                                                                            >  NB501016:230:HFVJLAFXY:3:21608:1829:18754/1‑151 (MQ=6)
                                                       GCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCA                                                                                          <  NB501016:230:HFVJLAFXY:1:21308:5166:13572/151‑1 (MQ=6)
                                                        CGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCT                                                                                                          <  NB501016:230:HFVJLAFXY:4:21405:17222:14519/134‑1 (MQ=20)
                                                         GGGTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGC                                                                                        <  NB501016:230:HFVJLAFXY:1:21205:15122:9281/151‑1 (MQ=6)
                                                          GTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCT                                                                                       <  NB501016:230:HFVJLAFXY:2:21303:17338:19045/151‑1 (MQ=6)
                                                          GTTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAAAGTTCCTGGCTGGAGATCTGCCTCGATGGCCTGCCGCAGTACTTCAACCTCCCCGCGCAGCT                                                                                       >  NB501016:230:HFVJLAFXY:4:11406:13601:16304/1‑151 (MQ=5)
                                                           TTTCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTT                                                                                      >  NB501016:230:HFVJLAFXY:2:21312:17555:14222/1‑151 (MQ=6)
                                                             TCCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTT                                                                                    >  NB501016:230:HFVJLAFXY:3:11506:16636:18621/1‑151 (MQ=6)
                                                              CCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTC                                                                                   >  NB501016:230:HFVJLAFXY:1:11304:19490:1891/1‑151 (MQ=6)
                                                              CCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTT                                                                                    <  NB501016:230:HFVJLAFXY:4:11607:17806:4413/150‑1 (MQ=6)
                                                              CCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTT                                                                                    <  NB501016:230:HFVJLAFXY:1:11102:11982:2159/150‑1 (MQ=6)
                                                              CCACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTT                                                                                    <  NB501016:230:HFVJLAFXY:2:21308:21373:13623/150‑1 (MQ=6)
                                                               CACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                                                                                  >  NB501016:230:HFVJLAFXY:3:21509:19040:8669/1‑151 (MQ=6)
                                                               CACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                                                                                  <  NB501016:230:HFVJLAFXY:1:21203:22034:18389/151‑1 (MQ=6)
                                                               CACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATACTTCCTG                                                                                                                                             >  NB501016:230:HFVJLAFXY:2:21204:18055:5570/1‑92 (MQ=8)
                                                                ACCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGT                                                                                 >  NB501016:230:HFVJLAFXY:1:21303:8887:6173/1‑151 (MQ=6)
                                                                 CCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTT                                                                                     >  NB501016:230:HFVJLAFXY:4:21410:9063:3441/1‑146 (MQ=6)
                                                                 CCACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGGACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTGTCGTT                                                                                >  NB501016:230:HFVJLAFXY:3:11406:9112:16615/1‑151 (MQ=6)
                                                                  CACTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTC                                                                               <  NB501016:230:HFVJLAFXY:4:11512:9275:16366/151‑1 (MQ=6)
                                                                   ACTTCCGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCT                                                                              >  NB501016:230:HFVJLAFXY:2:21103:7964:6870/1‑151 (MQ=6)
                                                                    CTTCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTC                                                                             >  NB501016:230:HFVJLAFXY:4:21402:12296:5571/1‑151 (MQ=6)
                                                                      TCAGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTC                                                                             <  NB501016:230:HFVJLAFXY:1:21101:10818:5827/149‑1 (MQ=6)
                                                                        AGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATT                                                                         <  NB501016:230:HFVJLAFXY:4:21506:2534:17223/151‑1 (MQ=6)
                                                                        AGCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATT                                                                         <  NB501016:230:HFVJLAFXY:2:11301:13331:16061/151‑1 (MQ=6)
                                                                         GCGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTT                                                                        >  NB501016:230:HFVJLAFXY:3:21506:10956:17071/1‑151 (MQ=6)
                                                                          CGGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTC                                                                       <  NB501016:230:HFVJLAFXY:1:11209:11090:10597/151‑1 (MQ=6)
                                                                           GGAGTCTCTGTCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCA                                                                      <  NB501016:230:HFVJLAFXY:2:21303:5942:2676/151‑1 (MQ=6)
                                                                           GGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCA                                                                      <  NB501016:230:HFVJLAFXY:1:21106:21710:7627/151‑1 (MQ=6)
                                                                           GGAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCA                                                                      <  NB501016:230:HFVJLAFXY:1:21202:16530:7403/151‑1 (MQ=6)
                                                                            GAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                                                                     <  NB501016:230:HFVJLAFXY:1:11310:4931:6320/151‑1 (MQ=6)
                                                                            GAGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                                                                     <  NB501016:230:HFVJLAFXY:3:11502:6011:10895/151‑1 (MQ=6)
                                                                             AGTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCCCGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGACCTGCCGCAGTTCTTCAACCGCCCGGCGCAGCTATTCGTGCTCAATTTCAGC                                                                    >  NB501016:230:HFVJLAFXY:3:11401:18546:11276/1‑151 (MQ=5)
                                                                              GTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCA                                                                   <  NB501016:230:HFVJLAFXY:2:21212:13040:9100/151‑1 (MQ=6)
                                                                              GTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCA                                                                   <  NB501016:230:HFVJLAFXY:1:11211:10888:15477/151‑1 (MQ=6)
                                                                              GTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCA                                                                   <  NB501016:230:HFVJLAFXY:4:11403:14966:9436/151‑1 (MQ=6)
                                                                              GTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCA                                                                   <  NB501016:230:HFVJLAFXY:4:11409:25789:5112/151‑1 (MQ=6)
                                                                              GTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGC                                                                    >  NB501016:230:HFVJLAFXY:4:11511:14455:20064/1‑150 (MQ=6)
                                                                              GTCTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGC                                                                    >  NB501016:230:HFVJLAFXY:3:11612:13047:5968/1‑150 (MQ=6)
                                                                               ggctcttgcattcttctgtTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCTATAGTTCCTGGCTGGAGTTCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCA                                                                   <  NB501016:230:HFVJLAFXY:1:21210:20456:6231/132‑1 (MQ=7)
                                                                                CTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCCACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                                                                 >  NB501016:230:HFVJLAFXY:4:11501:13068:19117/1‑151 (MQ=6)
                                                                                CTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                                                                 >  NB501016:230:HFVJLAFXY:1:11106:15076:13268/1‑151 (MQ=6)
                                                                                CTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                                                                 >  NB501016:230:HFVJLAFXY:4:11404:19079:18168/1‑151 (MQ=6)
                                                                                CTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                                                                 >  NB501016:230:HFVJLAFXY:3:11610:2480:9396/1‑151 (MQ=6)
                                                                                CTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                                                                 >  NB501016:230:HFVJLAFXY:2:11310:2515:4689/1‑151 (MQ=6)
                                                                                CTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                                                                 >  NB501016:230:HFVJLAFXY:4:21602:4550:17424/1‑151 (MQ=6)
                                                                                CTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                                                                                 >  NB501016:230:HFVJLAFXY:1:11208:11353:7078/1‑87 (MQ=9)
                                                                                CTCTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGAGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                                                                 >  NB501016:230:HFVJLAFXY:1:21307:2803:5231/1‑151 (MQ=6)
                                                                                CTCTGGCATTC‑TTCAGTTCCTGTCCGTCGGCCTGCGCACGCGTAAGTGGATGGCGTTCGTACTCAATAGTTCCTGCCTGGACATCTGCCTCGCTGTCCTGCCGAAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                                                                 >  NB501016:230:HFVJLAFXY:3:11608:26730:16371/1‑151 (MQ=5)
                                                                                  CTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC                                                               >  NB501016:230:HFVJLAFXY:2:21206:20857:10943/1‑151 (MQ=6)
                                                                                  CTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC                                                               >  NB501016:230:HFVJLAFXY:3:21412:17668:10946/1‑151 (MQ=6)
                                                                                  CTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC                                                               >  NB501016:230:HFVJLAFXY:1:21206:18479:7613/1‑151 (MQ=6)
                                                                                  CTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC                                                               >  NB501016:230:HFVJLAFXY:1:11203:5829:12803/1‑151 (MQ=6)
                                                                                  CTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCAT                                                                  <  NB501016:230:HFVJLAFXY:3:11510:10906:7384/148‑1 (MQ=6)
                                                                                  CTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCAT                                                                  <  NB501016:230:HFVJLAFXY:1:21302:19697:14395/148‑1 (MQ=6)
                                                                                  CTGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                                                                     >  NB501016:230:HFVJLAFXY:3:11603:22500:11989/1‑145 (MQ=7)
                                                                                   TGGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCT                                                              >  NB501016:230:HFVJLAFXY:1:21304:4959:6191/1‑151 (MQ=6)
                                                                                    GGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGCGGGCGGTCGTACTCAATAGTTCCTCGCGGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCAGCCC                                                               <  NB501016:230:HFVJLAFXY:3:11605:7372:20333/149‑1 (MQ=5)
                                                                                    GGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTT                                                             >  NB501016:230:HFVJLAFXY:4:11606:1628:14433/1‑151 (MQ=6)
                                                                                    GGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCT                                                              <  NB501016:230:HFVJLAFXY:1:21105:18877:19073/150‑1 (MQ=6)
                                                                                    GGCATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC                                                               <  NB501016:230:HFVJLAFXY:4:21504:23543:5255/149‑1 (MQ=6)
                                                                                      CATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCACTTTCAGCATCCCTTTC                                                           >  NB501016:230:HFVJLAFXY:1:11112:6432:9895/1‑151 (MQ=6)
                                                                                      CATTC‑TTAAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAAAGTTACTGGCTGGAGATATGCCTCCCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTATCGTTCTCAATTTCAGCATCCCTTTC                                                           >  NB501016:230:HFVJLAFXY:4:21407:20345:9891/1‑151 (MQ=5)
                                                                                       ATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG                                                          <  NB501016:230:HFVJLAFXY:4:11602:5332:6267/151‑1 (MQ=6)
                                                                                       ATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG                                                          <  NB501016:230:HFVJLAFXY:3:21401:22162:3226/151‑1 (MQ=6)
                                                                                       ATTC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG                                                          <  NB501016:230:HFVJLAFXY:2:21205:3848:12339/151‑1 (MQ=6)
                                                                                        TTTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCA                                                                            >  NB501016:230:HFVJLAFXY:4:11408:22297:19819/1‑133 (MQ=7)
                                                                                         TC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCGTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGG                                                         <  NB501016:230:HFVJLAFXY:3:11411:13851:7074/150‑1 (MQ=6)
                                                                                         TC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC                                                        >  NB501016:230:HFVJLAFXY:1:11101:10299:7719/1‑151 (MQ=7)
                                                                                         TC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGG                                                         <  NB501016:230:HFVJLAFXY:2:21210:24216:8462/150‑1 (MQ=7)
                                                                                         TC‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGGTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGG                                                         <  NB501016:230:HFVJLAFXY:3:11504:24761:17386/150‑1 (MQ=6)
                                                                                          C‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC                                                        >  NB501016:230:HFVJLAFXY:2:11304:19178:4527/1‑150 (MQ=7)
                                                                                          C‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC                                                        >  NB501016:230:HFVJLAFXY:4:21512:1404:10731/1‑150 (MQ=7)
                                                                                          C‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC                                                        >  NB501016:230:HFVJLAFXY:3:21408:8031:2003/1‑150 (MQ=7)
                                                                                          C‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAAGTTCAGCATCCCTTTCGGC                                                        >  NB501016:230:HFVJLAFXY:2:11204:8899:2115/1‑150 (MQ=6)
                                                                                          C‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTC                                                                               >  NB501016:230:HFVJLAFXY:2:11203:1650:4630/1‑127 (MQ=7)
                                                                                          C‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                                                                                  >  NB501016:230:HFVJLAFXY:3:21601:24384:8443/1‑124 (MQ=7)
                                                                                          C‑TTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCT                                                                                       <  NB501016:230:HFVJLAFXY:1:11305:26572:3707/119‑1 (MQ=7)
                                                                                           cttcagttcaTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGATCTTCAACCTCCCGGAGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC                                                        >  NB501016:230:HFVJLAFXY:3:21602:5781:10021/11‑150 (MQ=7)
                                                                                             TCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCAT                                                      >  NB501016:230:HFVJLAFXY:2:21309:8133:3422/1‑150 (MQ=7)
                                                                                             TCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC                                                        <  NB501016:230:HFVJLAFXY:2:11309:1470:6722/148‑1 (MQ=7)
                                                                                             TCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCAT                                                      <  NB501016:230:HFVJLAFXY:1:21107:26768:5639/150‑1 (MQ=6)
                                                                                               AGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACC                                                   >  NB501016:230:HFVJLAFXY:1:11206:20795:1695/1‑151 (MQ=7)
                                                                                                GTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACC                                                   >  NB501016:230:HFVJLAFXY:4:21509:19328:18380/1‑150 (MQ=7)
                                                                                                  TCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATT                                                >  NB501016:230:HFVJLAFXY:2:11201:4991:5621/1‑151 (MQ=5)
                                                                                                  TCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATT                                                >  NB501016:230:HFVJLAFXY:4:11608:2826:4440/1‑151 (MQ=5)
                                                                                                   CCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATTT                                               <  NB501016:230:HFVJLAFXY:3:11607:22705:16642/151‑1 (MQ=3)
                                                                                                   CCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATTT                                               <  NB501016:230:HFVJLAFXY:2:11111:11590:9341/151‑1 (MQ=3)
                                                                                                   CCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATTT                                               >  NB501016:230:HFVJLAFXY:3:21607:13103:14464/1‑151 (MQ=3)
                                                                                                   CCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTC                                                                               <  NB501016:230:HFVJLAFXY:3:21508:19401:16286/119‑1 (MQ=8)
                                                                                                   CCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGATTTTCGTTCTCAATTTCAGCATCCCTTTCGGAATACCATTT                                               >  NB501016:230:HFVJLAFXY:1:11108:2569:5946/1‑151 (MQ=3)
                                                                                                    CTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCGGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGagctcggaagcg                                              >  NB501016:230:HFVJLAFXY:2:11105:20941:16753/1‑139 (MQ=7)
                                                                                                     TGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGGAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATTTT                                              >  NB501016:230:HFVJLAFXY:1:11212:23085:14891/1‑150 (MQ=1)
                                                                                                     TGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATTTT                                              >  NB501016:230:HFVJLAFXY:1:21306:2344:8449/1‑150 (MQ=1)
                                                                                                     GGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATTTT                                              >  NB501016:230:HFVJLAFXY:4:21502:23889:9751/1‑150 (MQ=1)
                                                                                                      GTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATTTTAT                                            <  NB501016:230:HFVJLAFXY:4:11612:23953:3806/151‑1 (MQ=0)
                                                                                                      GTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATTTTA                                             <  NB501016:230:HFVJLAFXY:3:11405:21759:6177/150‑1 (MQ=0)
                                                                                                      GTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATTTTA                                             <  NB501016:230:HFVJLAFXY:2:11307:20936:7081/150‑1 (MQ=0)
                                                                                                         CGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTacaggccgatgccgctgaactggatgcgttg                                          >  NB501016:230:HFVJLAFXY:4:21511:13941:7145/1‑119 (MQ=1)
                                                                                                                  GCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCC                                                                                                >  NB501016:230:HFVJLAFXY:3:11412:10421:16660/1‑87 (MQ=0)
                                                                                                                             AGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATCTTATAACGGCGGCAGAGTCATAAAGC                      >  NB501016:230:HFVJLAFXY:1:11103:10795:4447/1‑150 (MQ=43)
                                                                                                                                  ATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATCTTATAACGGCGGCAGAGTCATAAAGCACCTC                 >  NB501016:230:HFVJLAFXY:2:11109:18911:18121/1‑150 (MQ=43)
                                                                                                                                                       |                                                                                                                                               
GTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTT‑TTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCATCTTATAACGGCGGCAGAGTCATAAAGCACCTCATTACCCTTGCCACC  >  NC_000913/1635978‑1636271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑