Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,636,503 T→C 93.9% intergenic (‑136/‑253) nohQ ← / → ynfO pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,636,5030TC93.9% 1796.5 / 60.8 778intergenic (‑136/‑253)nohQ/ynfOpseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage
Reads supporting (aligned to +/- strand):  ref base T (23/25);  new base C (357/366);  total (384/394)
Fisher's exact test for biased strand distribution p-value = 8.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 778 total aligned reads displayed.

ACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAA‑AAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTC  >  NC_000913/1636360‑1636648
                                                                                                                                                |                                                                                                                                                 
aCTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAA‑AAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAATGCCt                                                                                                                                               <  3:94868/148‑1 (MQ=11)
     cATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCAAA‑AAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAcc                                                                                                                                         >  7:91303/1‑149 (MQ=11)
                                                gagCCCAACAAGCTCGCTATCAGCACCTGTCGTTTACGTTCTTTTGAGAGTGTATTTTAAATAAAAACAATTACTAATGACGAAGAAGAACGGAAAAGCCTTAACCGTTAAAATTTTCATAAATAGCGAAAACCCGCGAGTTcgccggc                                                                                               <  2:241642/147‑3 (MQ=2)
                                                agGCCCAA‑AAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGccc                                                                                              <  5:53733/149‑1 (MQ=255)
                                                     cAA‑AAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTaa                                                                                         <  2:16884/149‑1 (MQ=40)
                                                               gctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCtt                                                                                                                                              <  4:119251/85‑1 (MQ=11)
                                                               gTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGa                                                                                >  6:376556/1‑149 (MQ=38)
                                                                ctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAAc                                                                                        <  3:280008/139‑1 (MQ=9)
                                                                ctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCCAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCTGAt                                                                               <  4:184957/148‑1 (MQ=11)
                                                                 tttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGAt                                                                               >  1:326848/1‑148 (MQ=12)
                                                                 tttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCCAGGTcgcc                                                                                                  <  8:110790/129‑1 (MQ=255)
                                                                   tCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                                               <  2:387647/130‑1 (MQ=12)
                                                                    cAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAAt                                                                                                                                  >  2:332858/1‑94 (MQ=11)
                                                                    cAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCAcc                                                                           >  4:93717/1‑149 (MQ=255)
                                                                    cAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCAc                                                                            <  5:19118/148‑1 (MQ=16)
                                                                       cACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                                               <  6:40760/126‑1 (MQ=12)
                                                                            gTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAAccc                                                                                                             <  5:278431/107‑1 (MQ=255)
                                                                            gTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGa                                                                   >  2:7897/1‑149 (MQ=255)
                                                                             tCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTc                                                                                                                              <  4:19619/89‑1 (MQ=11)
                                                                               gTTTGCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCGTTAAACCGGAAAATTTTCATAAATAGCGAAAAGCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGTAcc                                                                 <  4:210154/148‑1 (MQ=11)
                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAAccc                                                                                                             <  5:382618/104‑1 (MQ=255)
                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                                                                <  4:268993/149‑1 (MQ=255)
                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                                                                >  5:319627/1‑149 (MQ=255)
                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                                                                >  6:212385/1‑149 (MQ=255)
                                                                                tttCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCg                                                               <  2:203237/149‑1 (MQ=255)
                                                                                tttCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCg                                                               >  8:225247/1‑149 (MQ=255)
                                                                                 ttCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGCCGAAGAAGAACGGAACCGCCTTAAACCGGAAAATTTTCATATATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCg                                                               >  1:24909/1‑148 (MQ=11)
                                                                                  tCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGc                                                                                                                     >  1:56192/1‑93 (MQ=11)
                                                                                  tCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGt                                                              <  7:360891/148‑1 (MQ=255)
                                                                                    ctttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTa                                                             >  3:308352/1‑147 (MQ=255)
                                                                                    ctttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGCCGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTa                                                             >  4:325512/1‑147 (MQ=255)
                                                                                    ctttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGCACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTa                                                             >  6:186057/1‑147 (MQ=255)
                                                                                    ctttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTa                                                             >  6:261042/1‑147 (MQ=255)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTc                                                                                                                              <  1:160866/81‑1 (MQ=11)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGa                                                                                                         <  2:272795/102‑1 (MQ=255)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                                               <  8:373598/112‑1 (MQ=255)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCa                                                                             <  7:126005/130‑1 (MQ=255)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg                                                          >  2:11490/1‑149 (MQ=255)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg                                                          >  1:354248/1‑149 (MQ=255)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg                                                          >  3:61828/1‑149 (MQ=255)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg                                                          >  6:319368/1‑149 (MQ=255)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTATCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                                                                     <  7:185992/138‑1 (MQ=255)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAAATTTCATAAATAGCGAAAACACGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTaaat                                                          >  6:170718/1‑148 (MQ=11)
                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAACGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTcgccg                                                                                                 >  5:172742/1‑110 (MQ=11)
                                                                                     tttatttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGATACGACTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGACGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg                                                          >  5:248898/1‑149 (MQ=14)
                                                                                       tctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                                         <  1:379556/148‑1 (MQ=255)
                                                                                       tctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                                         <  8:306541/148‑1 (MQ=255)
                                                                                        ctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                                               >  2:357403/1‑109 (MQ=255)
                                                                                         ttttCAGAGGGTATTTTAAATAAAAACTTTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGa                                                       >  2:6518/1‑148 (MQ=255)
                                                                                         ttttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                                               <  4:284987/108‑1 (MQ=255)
                                                                                         ttttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGa                                                       >  1:98883/1‑148 (MQ=255)
                                                                                           ttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAt                                                      <  6:332138/147‑1 (MQ=255)
                                                                                           ttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAt                                                      <  8:47123/147‑1 (MQ=255)
                                                                                            tCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATaa                                                    <  7:221345/148‑1 (MQ=255)
                                                                                            tCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATaa                                                    <  5:207692/148‑1 (MQ=255)
                                                                                              agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                                                                <  8:336781/131‑1 (MQ=255)
                                                                                              agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGa                                                 <  7:246506/146‑1 (MQ=255)
                                                                                              agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGa                                                 >  7:387780/4‑149 (MQ=255)
                                                                                              agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGa                                                 >  1:182861/4‑149 (MQ=255)
                                                                                              agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGa                                                 <  3:204762/146‑1 (MQ=255)
                                                                                               gagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAcc                                                                                                                                         >  5:90942/3‑60 (MQ=255)
                                                                                               gagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAt                                                >  2:141741/3‑149 (MQ=255)
                                                                                                agggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCGTAAACCGGTAAATTTTCATAAAGAGAGAAAACCCTCGAGGTCGCCGCCACGGAACATGTCGGATCACCGGAAAGGACCCGTAATGTGATAATGAt                                                >  6:350762/2‑148 (MQ=255)
                                                                                                 gggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTa                                              >  6:62742/1‑149 (MQ=255)
                                                                                                  ggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCg                                                                                  <  3:140316/112‑1 (MQ=12)
                                                                                                  ggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCAc                                                                            >  7:63024/1‑118 (MQ=19)
                                                                                                   gTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTatc                                            <  6:282719/149‑1 (MQ=255)
                                                                                                   gTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTatc                                            <  3:336757/149‑1 (MQ=255)
                                                                                                      ttttAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTatcat                                          <  7:324963/148‑1 (MQ=255)
                                                                                                           aataaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGTGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACa                                     >  5:232030/1‑148 (MQ=255)
                                                                                                           aataaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGCGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCGACa                                     >  6:246273/1‑148 (MQ=255)
                                                                                                           aataaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACa                                     >  8:26441/1‑148 (MQ=255)
                                                                                                              aaaaaCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGAAAAGGACCCGTAAAGTGATAATGAtt                                               >  2:99854/1‑135 (MQ=255)
                                                                                                               aaaaCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAACCCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATca                                >  6:132851/1‑149 (MQ=255)
                                                                                                                     ttAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgt                          <  3:196012/149‑1 (MQ=255)
                                                                                                                        aGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTa                                              <  1:87871/126‑1 (MQ=255)
                                                                                                                        aGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgc                        <  8:8354/148‑1 (MQ=255)
                                                                                                                         gTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgt                      <  1:100139/149‑1 (MQ=255)
                                                                                                                          ttATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATg                                                  <  7:103912/120‑1 (MQ=255)
                                                                                                                          ttATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgtg                     <  5:34978/149‑1 (MQ=255)
                                                                                                                          ttATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGGCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgtg                     <  2:304515/149‑1 (MQ=255)
                                                                                                                          ttACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgtg                     <  1:128215/149‑1 (MQ=255)
                                                                                                                               acgaAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGc                >  7:287723/1‑149 (MQ=255)
                                                                                                                                cgaAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGc                >  5:261461/1‑148 (MQ=255)
                                                                                                                                  aagaagaaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCTCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCAt             <  5:146772/149‑1 (MQ=255)
                                                                                                                                  aagaagaaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTaaa                                                           >  3:218737/1‑103 (MQ=255)
                                                                                                                                  aagaagaaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCAt             >  4:226774/1‑149 (MQ=255)
                                                                                                                                   agaagaaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCAt             >  1:199954/1‑148 (MQ=255)
                                                                                                                                     aagaaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCa              <  3:239691/145‑1 (MQ=255)
                                                                                                                                       gaaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAAc        >  4:132641/1‑149 (MQ=255)
                                                                                                                                       gaaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGCAAAGGACCCCTAAAGAGATAATCATTATCAACAACATAACACAACGAGCGTGCAGGCCATCAAAc        >  4:250630/1‑149 (MQ=14)
                                                                                                                                        aaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAAcc       <  8:355996/149‑1 (MQ=255)
                                                                                                                                        aaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAAc        >  8:198240/1‑148 (MQ=255)
                                                                                                                                        aaCGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAAc        >  7:4732/1‑148 (MQ=255)
                                                                                                                                          cGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAtt                                               <  1:291732/107‑1 (MQ=255)
                                                                                                                                          cGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCAc     >  8:158574/1‑149 (MQ=255)
                                                                                                                                          cGGAAACGCCTTAAACCGGAAAATTTCCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAtt                                               >  4:37245/1‑107 (MQ=255)
                                                                                                                                             aaaCGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATa                                                     <  5:368251/98‑1 (MQ=255)
                                                                                                                                             aaaCGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGa                   >  3:180390/1‑132 (MQ=255)
                                                                                                                                             aaaCGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGt   >  1:222769/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAA‑AAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTC  >  NC_000913/1636360‑1636648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 458 total aligned reads displayed.
TTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAAT  >  NC_000913/1636354‑1636652
                                                                                                                                                     |                                                                                                                                                     
    ttagTTCCATTGTTCATTCGAAGGACAATAACAGATCAAGGAAACGACCTAGGCCAAAAAGCTCGATTTAAGAACCTGTCTTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCT                                                                                                                                                   <  NB501016:230:HFVJLAFXY:3:21402:26367:3974/146‑1 (MQ=0)
    TGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTT                                                                                                                                                  >  NB501016:230:HFVJLAFXY:4:21603:17082:6382/1‑151 (MQ=0)
     GACTTCCATTGTTCATTCCACGGACAAAAACAGTGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                                                                 <  NB501016:230:HFVJLAFXY:3:11509:15304:6356/151‑1 (MQ=0)
     GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAATCGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTT                                                                                                                                                  <  NB501016:230:HFVJLAFXY:4:11605:7045:7674/150‑1 (MQ=0)
     GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAATGCCTT                                                                                                                                                  <  NB501016:230:HFVJLAFXY:2:11207:21946:18931/150‑1 (MQ=8)
     GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                                                                 <  NB501016:230:HFVJLAFXY:4:11509:5250:15810/151‑1 (MQ=0)
     GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                                                                 <  NB501016:230:HFVJLAFXY:4:11608:9105:8864/151‑1 (MQ=0)
     GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTT                                                                                                                                                  <  NB501016:230:HFVJLAFXY:4:11602:8963:5287/150‑1 (MQ=0)
      ACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTTGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAA                                                                                                                                                <  NB501016:230:HFVJLAFXY:4:11602:15535:3547/151‑1 (MQ=0)
       CTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGGGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAA                                                                                                                                                >  NB501016:230:HFVJLAFXY:4:21401:5335:19164/1‑150 (MQ=0)
       CTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAA                                                                                                                                                >  NB501016:230:HFVJLAFXY:1:11102:4071:11022/1‑150 (MQ=0)
       CTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTGTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGACCGGAAACGCCTTAA                                                                                                                                                >  NB501016:230:HFVJLAFXY:1:11209:3661:2194/1‑150 (MQ=0)
        TTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTA                                                                                                                                                 >  NB501016:230:HFVJLAFXY:1:21301:12722:10593/1‑148 (MQ=60)
          CCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCG                                                                                                                                            >  NB501016:230:HFVJLAFXY:3:21402:4954:18809/1‑151 (MQ=60)
           CATTGTTCATTCCACGGCCAAAAACAGATAAAGGAAACGACAGAGGCCCAAAATCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCATGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGG                                                                                                                                           <  NB501016:230:HFVJLAFXY:2:21112:19887:2913/151‑1 (MQ=60)
                         CGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAA                                                                                                                              >  NB501016:230:HFVJLAFXY:1:11311:23511:13079/1‑150 (MQ=60)
                          cgaacaaaacacactaaagaaaagacacacgccaaaaaccccgcctttaaccacatgagttttcatttctttaatacgggaattttAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAAT                                                                                                                            <  NB501016:230:HFVJLAFXY:3:11411:19460:7890/65‑1 (MQ=11)
                                              atacgagatcatagccggtgactggagttcagacgtgtgctccttccgatctcagaGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCG                                                                                                        <  NB501016:230:HFVJLAFXY:2:21203:18338:9036/95‑1 (MQ=9)
                                                    ccttGCCCAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                                                  <  NB501016:230:HFVJLAFXY:2:11109:10618:14899/147‑1 (MQ=5)
                                                           gaaagcgagctttttggcctCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACC                                                                                           <  NB501016:230:HFVJLAFXY:4:11405:16810:18007/131‑1 (MQ=7)
                                                            AAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCAGCGAGGTCGCCGCCCCGTAAGCT                                                                                          >  NB501016:230:HFVJLAFXY:3:11503:12326:16760/1‑151 (MQ=0)
                                                             AAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTG                                                                                         <  NB501016:230:HFVJLAFXY:4:11507:23954:11362/151‑1 (MQ=0)
                                                              AAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGATACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                                                  >  NB501016:230:HFVJLAFXY:2:11209:11519:3928/1‑141 (MQ=0)
                                                              AAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGT                                                                                        <  NB501016:230:HFVJLAFXY:1:11311:6464:17909/151‑1 (MQ=0)
                                                              AAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGA                                                                                                                       <  NB501016:230:HFVJLAFXY:3:11408:12909:8036/120‑1 (MQ=0)
                                                               AGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTC                                                                                       >  NB501016:230:HFVJLAFXY:2:21103:19552:17864/1‑151 (MQ=43)
                                                                 CTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGagatcggaaagagcacacgtctgaact                                                                                     >  NB501016:230:HFVJLAFXY:3:11603:5344:15361/1‑124 (MQ=0)
                                                                 CTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTAGGACGAAGAAGAACGGAACCGCCTTAACCCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTCACCTGTCGG                                                                                     >  NB501016:230:HFVJLAFXY:1:21112:23804:3480/1‑151 (MQ=0)
                                                                  TCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGG                                                                                                                                           >  NB501016:230:HFVJLAFXY:2:21304:21370:13473/1‑96 (MQ=0)
                                                                   CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGAT                                                                                   <  NB501016:230:HFVJLAFXY:3:11407:4615:18565/151‑1 (MQ=1)
                                                                   CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGAT                                                                                   <  NB501016:230:HFVJLAFXY:1:11107:14939:8628/151‑1 (MQ=1)
                                                                   CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGAT                                                                                   >  NB501016:230:HFVJLAFXY:1:21101:18957:17152/1‑151 (MQ=1)
                                                                   CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGG                                                                                     <  NB501016:230:HFVJLAFXY:2:21309:15141:3722/149‑1 (MQ=1)
                                                                   CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGG                                                                                     <  NB501016:230:HFVJLAFXY:3:11606:25501:15209/149‑1 (MQ=1)
                                                                   CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCG                                                                                                                >  NB501016:230:HFVJLAFXY:3:11410:11589:4912/1‑122 (MQ=1)
                                                                   CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                                                                 >  NB501016:230:HFVJLAFXY:2:21102:1989:9894/1‑89 (MQ=2)
                                                                   CGCTTTCAGCACCAGTCGTTTCCTTTCTTTTCAGAGGGTAATTTAAATAAAAACAATAAGTTATGAAGAAGAAGAACGGAAACGCCTTAAACCGTAAAATTATCAAATCTAGCGAAAACCCTCGAGGTCGCCGCCCAGTAACATGTCGGA                                                                                    >  NB501016:230:HFVJLAFXY:3:21603:4553:9691/1‑150 (MQ=0)
                                                                    GTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATC                                                                                  <  NB501016:230:HFVJLAFXY:1:11201:22453:3994/151‑1 (MQ=37)
                                                                    GCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACC                                                                                           <  NB501016:230:HFVJLAFXY:2:21207:17427:18274/142‑1 (MQ=3)
                                                                     CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATC                                                                                  >  NB501016:230:HFVJLAFXY:1:21211:11502:17798/1‑150 (MQ=5)
                                                                     CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATC                                                                                  >  NB501016:230:HFVJLAFXY:4:11402:1463:10841/1‑150 (MQ=5)
                                                                     CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATC                                                                                  >  NB501016:230:HFVJLAFXY:4:21611:12590:18080/1‑150 (MQ=5)
                                                                     CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGAGAGGTCG                                                                                                        >  NB501016:230:HFVJLAFXY:4:11411:9049:1480/1‑128 (MQ=5)
                                                                     CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAAACCGCGAGGTCGCCTCCCAGTAACCTGTCGGATC                                                                                  >  NB501016:230:HFVJLAFXY:2:11105:10581:2355/1‑150 (MQ=4)
                                                                        TCAGCACCTGTCGTTTCCTTTCTTCTCCGAGGGTATAGTAAATAAACACATTAAGGTATGACGAACAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCTCCGCCCCGCAACCTGTCGGATCACC                                                                               <  NB501016:230:HFVJLAFXY:3:21412:5296:3505/150‑1 (MQ=2)
                                                                         CAGCTCCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGTAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGG                                                                             <  NB501016:230:HFVJLAFXY:1:21202:20903:8663/151‑1 (MQ=19)
                                                                         CAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGG                                                                             <  NB501016:230:HFVJLAFXY:1:11106:2020:19163/151‑1 (MQ=20)
                                                                         CAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                                                  >  NB501016:230:HFVJLAFXY:3:21611:22975:18972/1‑130 (MQ=7)
                                                                           GCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGA                                                                            >  NB501016:230:HFVJLAFXY:4:21611:1880:5138/1‑150 (MQ=20)
                                                                           GCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                                                  >  NB501016:230:HFVJLAFXY:3:11408:25445:18634/1‑128 (MQ=7)
                                                                           GCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                                                  <  NB501016:230:HFVJLAFXY:3:21611:12175:13627/128‑1 (MQ=7)
                                                                            CACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAA                                                                           >  NB501016:230:HFVJLAFXY:4:11409:24428:1530/1‑150 (MQ=20)
                                                                             ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                                         <  NB501016:230:HFVJLAFXY:1:11110:19437:2044/151‑1 (MQ=20)
                                                                             ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                                         <  NB501016:230:HFVJLAFXY:3:21608:8699:2574/151‑1 (MQ=20)
                                                                             ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                                         <  NB501016:230:HFVJLAFXY:2:21108:13600:12436/151‑1 (MQ=20)
                                                                             ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                                         <  NB501016:230:HFVJLAFXY:2:21103:13980:7389/151‑1 (MQ=20)
                                                                             ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                                         <  NB501016:230:HFVJLAFXY:4:11502:24439:13116/151‑1 (MQ=20)
                                                                              CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATATCGAAAACCCGCGCGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                                                                        >  NB501016:230:HFVJLAFXY:1:11308:21985:19921/1‑151 (MQ=19)
                                                                              CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGG                                                                                                                                           <  NB501016:230:HFVJLAFXY:4:11505:25813:6974/84‑1 (MQ=9)
                                                                              CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCT                                                                                                                                                   <  NB501016:230:HFVJLAFXY:3:21601:3376:11865/76‑1 (MQ=7)
                                                                               CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                                                                        >  NB501016:230:HFVJLAFXY:4:11511:11308:17091/1‑150 (MQ=20)
                                                                               CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                                         <  NB501016:230:HFVJLAFXY:3:21511:22950:4006/149‑1 (MQ=20)
                                                                               CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                                                  <  NB501016:230:HFVJLAFXY:1:11306:21246:10060/124‑1 (MQ=8)
                                                                               CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAACGGAAAATTTTCATAAATAGCGAAAACACGCGAGGTCGCCGACCCGTAACCTGTCGGATCACCGGAAAGGC                                                                       >  NB501016:230:HFVJLAFXY:3:21407:16335:9815/1‑151 (MQ=18)
                                                                                TGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGCAAGGAC                                                                      >  NB501016:230:HFVJLAFXY:4:21411:25794:4782/1‑151 (MQ=19)
                                                                                 ccgctcTCCTTTCTTTTCAGGGGGTATTTTAACTAAAAACATTAAGTTACGACGAAGACGACCGGAAATGCCTTAAACCGGAAACTTTTCATAAATCGCGAAACCCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                                                                      <  NB501016:230:HFVJLAFXY:1:21205:1566:2891/144‑1 (MQ=37)
                                                                                 GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACC                                                                     <  NB501016:230:HFVJLAFXY:2:21104:12399:5400/151‑1 (MQ=20)
                                                                                 GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACC                                                                     >  NB501016:230:HFVJLAFXY:4:21412:22024:9471/1‑151 (MQ=20)
                                                                                 GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                                                                      <  NB501016:230:HFVJLAFXY:2:11309:5630:18224/150‑1 (MQ=20)
                                                                                 GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCA                                                                                                                                 >  NB501016:230:HFVJLAFXY:2:11104:17300:17099/1‑91 (MQ=9)
                                                                                   CGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGCACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCGGCGAGGTGGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCG                                                                   <  NB501016:230:HFVJLAFXY:1:11304:6998:13283/151‑1 (MQ=20)
                                                                                   CGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCG                                                                   <  NB501016:230:HFVJLAFXY:2:11203:9302:11727/151‑1 (MQ=22)
                                                                                   CGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCG                                                                                                                <  NB501016:230:HFVJLAFXY:3:21612:6823:11847/106‑1 (MQ=8)
                                                                                   CGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTC                                                                                                                                  >  NB501016:230:HFVJLAFXY:3:21406:18350:1808/1‑88 (MQ=9)
                                                                                   CGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACG                                                                                                                                                      >  NB501016:230:HFVJLAFXY:3:11511:7591:15376/1‑68 (MQ=8)
                                                                                    GTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGT                                                                  >  NB501016:230:HFVJLAFXY:4:21402:5561:3011/1‑151 (MQ=24)
                                                                                    GTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCAT                                                                  >  NB501016:230:HFVJLAFXY:2:11302:11004:6397/1‑151 (MQ=18)
                                                                                     TTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGCCGAAGAAGAACGGAACCGCCTTAAACCGGAAAATTTTCATATATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGT                                                                  >  NB501016:230:HFVJLAFXY:1:11104:23916:18466/1‑150 (MQ=22)
                                                                                     TTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGT                                                                  >  NB501016:230:HFVJLAFXY:3:21505:24330:12204/1‑150 (MQ=24)
                                                                                     TTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGT                                                                  >  NB501016:230:HFVJLAFXY:3:21603:15017:14285/1‑150 (MQ=24)
                                                                                      TTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAATAACGGAAACGCCTTAAAACGTAAAATTTTCATAAATAGCGAAAACCCTCGAGGTCGCCGCCCCGTAACCTGTCGTATCACCGGAAAGGACCCGTA                                                                 >  NB501016:230:HFVJLAFXY:4:21605:10786:8507/1‑150 (MQ=23)
                                                                                      TTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCG                                                                                                                        >  NB501016:230:HFVJLAFXY:1:11110:5797:5416/1‑95 (MQ=8)
                                                                                       TCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAA                                                                >  NB501016:230:HFVJLAFXY:3:11608:19978:18130/1‑150 (MQ=28)
                                                                                       TCCTTTCTTTTCAGAGGGTAATTTAAATAAAAACATTAAGTTATGACGAAGAAGAACCGAACAGCCTTAAACCGGAAAATTTTCATAAATAGAGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAA                                                                >  NB501016:230:HFVJLAFXY:1:11106:1844:3234/1‑150 (MQ=34)
                                                                                        CCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAA                                                                >  NB501016:230:HFVJLAFXY:2:21301:8819:12476/1‑149 (MQ=52)
                                                                                        CCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCG                                                                                                                                            >  NB501016:230:HFVJLAFXY:4:11612:13186:10314/1‑73 (MQ=9)
                                                                                         CTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCTCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                                                                      >  NB501016:230:HFVJLAFXY:1:11309:18072:4216/1‑142 (MQ=43)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCT                                                                                                                                 <  NB501016:230:HFVJLAFXY:1:11305:16110:18674/83‑1 (MQ=9)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                                             >  NB501016:230:HFVJLAFXY:2:11212:16561:14023/1‑151 (MQ=34)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                                             >  NB501016:230:HFVJLAFXY:2:11201:8906:10821/1‑151 (MQ=34)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                                             >  NB501016:230:HFVJLAFXY:1:11310:19805:2697/1‑151 (MQ=34)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                                             >  NB501016:230:HFVJLAFXY:2:11201:23027:7567/1‑151 (MQ=34)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                                             >  NB501016:230:HFVJLAFXY:2:21101:8371:10568/1‑151 (MQ=34)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                                             >  NB501016:230:HFVJLAFXY:4:21506:22022:19428/1‑151 (MQ=34)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                                                  >  NB501016:230:HFVJLAFXY:4:21608:15537:3241/1‑114 (MQ=8)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAG                                                                                                            >  NB501016:230:HFVJLAFXY:1:21201:14382:11604/1‑104 (MQ=8)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCG                                                                                                              >  NB501016:230:HFVJLAFXY:3:11405:12701:6343/1‑102 (MQ=8)
                                                                                         CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTATCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                                                                        <  NB501016:230:HFVJLAFXY:4:11609:25592:8866/140‑1 (MQ=20)
                                                                                         CTTTATTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGATACGACTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGACGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                                             >  NB501016:230:HFVJLAFXY:3:21411:6637:5243/1‑151 (MQ=30)
                                                                                          TTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTG                                                            >  NB501016:230:HFVJLAFXY:1:11202:8470:8630/1‑151 (MQ=36)
                                                                                          TTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTAATAAATAGCGAAAACCCGCGAGGTCGCAGCCCCGTAAACTGTCTGATCACCGGAAAGGACCCGTAAAGTG                                                            >  NB501016:230:HFVJLAFXY:3:11402:15395:1112/1‑151 (MQ=32)
                                                                                           TTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGG                                                                                                                                           >  NB501016:230:HFVJLAFXY:3:11511:3912:20146/1‑71 (MQ=10)
                                                                                            TCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                                                  <  NB501016:230:HFVJLAFXY:1:21305:5525:12455/111‑1 (MQ=8)
                                                                                            TCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCG                                                                                                                        <  NB501016:230:HFVJLAFXY:2:21101:16918:6586/89‑1 (MQ=9)
                                                                                             CTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAT                                                          >  NB501016:230:HFVJLAFXY:1:11206:21263:5176/1‑150 (MQ=40)
                                                                                              TTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATA                                                         >  NB501016:230:HFVJLAFXY:3:11505:18703:13191/1‑150 (MQ=42)
                                                                                               TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATA                                                         <  NB501016:230:HFVJLAFXY:3:11404:15172:12600/149‑1 (MQ=42)
                                                                                               TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAT                                                          <  NB501016:230:HFVJLAFXY:3:21607:23552:9088/148‑1 (MQ=40)
                                                                                               TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAT                                                          <  NB501016:230:HFVJLAFXY:4:21608:5392:9529/148‑1 (MQ=40)
                                                                                                TTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAAT                                                       <  NB501016:230:HFVJLAFXY:3:11610:24343:9927/150‑1 (MQ=46)
                                                                                                  CAGAGGTTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGCGATAATGAT                                                    <  NB501016:230:HFVJLAFXY:1:11205:5449:7515/151‑1 (MQ=40)
                                                                                                  CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT                                                    >  NB501016:230:HFVJLAFXY:3:11406:7485:13456/1‑151 (MQ=52)
                                                                                                  CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT                                                    <  NB501016:230:HFVJLAFXY:2:21104:18859:11484/151‑1 (MQ=52)
                                                                                                  CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT                                                    <  NB501016:230:HFVJLAFXY:4:21408:11509:8560/151‑1 (MQ=52)
                                                                                                  CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT                                                    <  NB501016:230:HFVJLAFXY:4:21509:10408:11031/151‑1 (MQ=52)
                                                                                                  CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGA                                                     <  NB501016:230:HFVJLAFXY:4:11508:15225:16973/150‑1 (MQ=50)
                                                                                                  CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCG                                                                   >  NB501016:230:HFVJLAFXY:4:21601:8933:7229/1‑136 (MQ=23)
                                                                                                  CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                                                  <  NB501016:230:HFVJLAFXY:1:21309:24049:17419/105‑1 (MQ=8)
                                                                                                  CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGC                                                                                                                                                     <  NB501016:230:HFVJLAFXY:3:11511:15798:6669/54‑1 (MQ=8)
                                                                                                   AGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAG                                                                                                                          >  NB501016:230:HFVJLAFXY:1:21102:20951:6564/1‑80 (MQ=9)
                                                                                                   AGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCG                                                                                                                                            >  NB501016:230:HFVJLAFXY:3:11506:13163:7261/1‑62 (MQ=10)
                                                                                                    GAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTA                                                  <  NB501016:230:HFVJLAFXY:1:11101:24388:17623/151‑1 (MQ=57)
                                                                                                     AGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTAT                                                 <  NB501016:230:HFVJLAFXY:1:11108:10370:16593/151‑1 (MQ=59)
                                                                                                     AGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTAT                                                 <  NB501016:230:HFVJLAFXY:1:11310:8434:5593/151‑1 (MQ=59)
                                                                                                     AGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTAT                                                 >  NB501016:230:HFVJLAFXY:4:11403:4463:10823/1‑151 (MQ=59)
                                                                                                      GGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGTAAACGCCTTAAACCGGATAATTTTCATAAATAGCGAAATCCCGCGAGGTCGCCGCCCagatcggaagagcacacgtctgatctccaagacaccggctatgatctcgt                                                >  NB501016:230:HFVJLAFXY:2:11212:24785:1582/1‑101 (MQ=7)
                                                                                                      GGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATC                                                >  NB501016:230:HFVJLAFXY:3:11411:8464:16403/1‑151 (MQ=60)
                                                                                                      GGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACC                                                                               >  NB501016:230:HFVJLAFXY:4:11411:12163:10623/1‑120 (MQ=18)
                                                                                                      GGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGG                                                                                     <  NB501016:230:HFVJLAFXY:2:11304:12029:15763/114‑1 (MQ=8)
                                                                                                      GGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAA                                                                                                                              >  NB501016:230:HFVJLAFXY:4:21612:20574:10726/1‑73 (MQ=10)
                                                                                                          ATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCT                                            <  NB501016:230:HFVJLAFXY:4:11503:8201:12581/151‑1 (MQ=60)
                                                                                                          ATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCT                                            <  NB501016:230:HFVJLAFXY:3:21411:21641:7424/151‑1 (MQ=60)
                                                                                                          ATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATC                                             <  NB501016:230:HFVJLAFXY:4:21511:24501:4135/150‑1 (MQ=60)
                                                                                                          ATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCAT                                              <  NB501016:230:HFVJLAFXY:1:21201:7440:5950/149‑1 (MQ=60)
                                                                                                          ATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCAGCGAGGTCGCAGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATC                                             >  NB501016:230:HFVJLAFXY:2:21208:15769:2781/1‑150 (MQ=58)
                                                                                                           TTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCT                                            >  NB501016:230:HFVJLAFXY:1:11212:21731:16249/1‑150 (MQ=60)
                                                                                                            TTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTA                                           <  NB501016:230:HFVJLAFXY:2:21207:7149:19365/150‑1 (MQ=60)
                                                                                                             TTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAC                                          <  NB501016:230:HFVJLAFXY:1:11101:15980:4289/150‑1 (MQ=60)
                                                                                                             TTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAC                                          >  NB501016:230:HFVJLAFXY:2:21107:12394:7903/1‑150 (MQ=60)
                                                                                                             TTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGC                                                                                                                         <  NB501016:230:HFVJLAFXY:1:21108:11583:2515/71‑1 (MQ=10)
                                                                                                             TTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAC                                          >  NB501016:230:HFVJLAFXY:3:21602:10254:13981/1‑150 (MQ=60)
                                                                                                              TAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGATGTCTCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACA                                         <  NB501016:230:HFVJLAFXY:1:11204:1915:9992/150‑1 (MQ=58)
                                                                                                              TAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACATGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACA                                         <  NB501016:230:HFVJLAFXY:2:11203:2283:1396/150‑1 (MQ=60)
                                                                                                              TAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACAT                                        >  NB501016:230:HFVJLAFXY:4:21606:7627:14829/1‑151 (MQ=60)
                                                                                                               AAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTACCCTGTCGGATCACCGTAAAGGACCCGTAAAGTGATAATGATTATCATCTACAT                                        >  NB501016:230:HFVJLAFXY:3:21610:14884:18938/1‑150 (MQ=58)
                                                                                                               AAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACAT                                        <  NB501016:230:HFVJLAFXY:3:11612:18935:15053/150‑1 (MQ=60)
                                                                                                               AAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCAGCCCCGTAACCTGTCGGATCACCagatcggaagagcacacgtctgactccagtcaccggctat                                       >  NB501016:230:HFVJLAFXY:1:21302:8060:2696/1‑111 (MQ=18)
                                                                                                               AAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACAT                                        <  NB501016:230:HFVJLAFXY:3:21601:20551:3425/150‑1 (MQ=60)
                                                                                                                 ATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGTAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATC                                     >  NB501016:230:HFVJLAFXY:3:21401:9627:11148/1‑151 (MQ=60)
                                                                                                                 ATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATC                                     >  NB501016:230:HFVJLAFXY:3:21605:5947:17117/1‑151 (MQ=60)
                                                                                                                 ATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATCATTATCActttacgggt                                     >  NB501016:230:HFVJLAFXY:4:11408:26035:16850/1‑141 (MQ=51)
                                                                                                                  TAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGAAAAGGACCCGTAAAGTGATAATGATTA                                                  <  NB501016:230:HFVJLAFXY:1:11206:7545:8677/137‑1 (MQ=57)
                                                                                                                  TAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATC                                                                                  >  NB501016:230:HFVJLAFXY:2:21306:12088:13535/1‑105 (MQ=9)
                                                                                                                   AAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCAC                                   <  NB501016:230:HFVJLAFXY:4:21605:14865:8646/151‑1 (MQ=60)
                                                                                                                   AAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCA                                    <  NB501016:230:HFVJLAFXY:4:21406:2252:7877/150‑1 (MQ=60)
                                                                                                                    AAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCAC                                   >  NB501016:230:HFVJLAFXY:1:11106:15156:20080/1‑150 (MQ=60)
                                                                                                                    AAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCA                                               <  NB501016:230:HFVJLAFXY:1:21208:4018:8118/138‑1 (MQ=59)
                                                                                                                     AAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAA                                 <  NB501016:230:HFVJLAFXY:1:21307:8744:19449/151‑1 (MQ=60)
                                                                                                                       ACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACG                               >  NB501016:230:HFVJLAFXY:3:11606:5134:12270/1‑151 (MQ=60)
                                                                                                                       ACATTAAGTTATGACGAAGAAGAACGGAAACGCATTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACG                               >  NB501016:230:HFVJLAFXY:2:21110:8750:16547/1‑151 (MQ=60)
                                                                                                                         ATTAAGTTATGACGAAGATGTACGGAAACGCCTTAAACCGGTAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTG                             <  NB501016:230:HFVJLAFXY:2:11306:18018:2639/151‑1 (MQ=56)
                                                                                                                          TTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCG                                                                                                                <  NB501016:230:HFVJLAFXY:1:21212:26280:9581/67‑1 (MQ=0)
                                                                                                                            AAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCG                           <  NB501016:230:HFVJLAFXY:2:21109:5105:7059/150‑1 (MQ=60)
                                                                                                                            AAGTTATGAAGAAGAAGAACGGAAACGCCTTAAACAGTAAAATTTTCATAAATAGCGAAAACCCGCGAGGTAGCCGCCCCGTAAACTGTCGGATAACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGT                          <  NB501016:230:HFVJLAFXY:1:21105:25205:11386/151‑1 (MQ=55)
                                                                                                                             AGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTG                         <  NB501016:230:HFVJLAFXY:3:21410:8608:16521/151‑1 (MQ=60)
                                                                                                                              GTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG                        >  NB501016:230:HFVJLAFXY:2:11106:23988:14235/1‑151 (MQ=60)
                                                                                                                              GTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG                        <  NB501016:230:HFVJLAFXY:4:11410:6303:18698/151‑1 (MQ=60)
                                                                                                                              GTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGA                                                     <  NB501016:230:HFVJLAFXY:4:11506:17472:13059/122‑1 (MQ=44)
                                                                                                                              GTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG                        <  NB501016:230:HFVJLAFXY:1:11211:8891:17274/151‑1 (MQ=60)
                                                                                                                                TATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG                        >  NB501016:230:HFVJLAFXY:3:21410:1330:6928/1‑149 (MQ=60)
                                                                                                                                 ATGACGAAGAAGAACGGAAACGCCTTACACCGGAAAATTTTCCTACATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGCGCCCCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG                     <  NB501016:230:HFVJLAFXY:3:21504:2265:19076/151‑1 (MQ=56)
                                                                                                                                 ATGACGAAGAAGAACGGAAACGCCTTAAACCGTAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCTCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAG                      <  NB501016:230:HFVJLAFXY:1:21103:3108:20012/150‑1 (MQ=60)
                                                                                                                                 ATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG                     <  NB501016:230:HFVJLAFXY:4:21404:12754:13503/151‑1 (MQ=60)
                                                                                                                                 ATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG                     <  NB501016:230:HFVJLAFXY:1:11307:2151:8674/151‑1 (MQ=60)
                                                                                                                                 ATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAG                      <  NB501016:230:HFVJLAFXY:3:21612:15407:16542/150‑1 (MQ=60)
                                                                                                                                 ATGACGAAGAAGAACCGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG                     <  NB501016:230:HFVJLAFXY:3:11611:7230:7185/151‑1 (MQ=60)
                                                                                                                                   GACGAAGAAGAACGGAAACGCCTTTAACAGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTAGCCGCCCCGTAACCTGTAGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGC                    <  NB501016:230:HFVJLAFXY:2:21211:4979:20361/150‑1 (MQ=60)
                                                                                                                                   GACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCC                   >  NB501016:230:HFVJLAFXY:4:21510:15006:8776/1‑151 (MQ=60)
                                                                                                                                   GACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGC                    <  NB501016:230:HFVJLAFXY:2:11302:4073:20248/150‑1 (MQ=60)
                                                                                                                                   GACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGAGCGTGGAGGCC                   >  NB501016:230:HFVJLAFXY:4:21504:24965:5355/1‑151 (MQ=60)
                                                                                                                                    ACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCA                  <  NB501016:230:HFVJLAFXY:3:11605:10435:8155/151‑1 (MQ=60)
                                                                                                                                    ACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCC                   >  NB501016:230:HFVJLAFXY:3:21502:7108:11424/1‑150 (MQ=60)
                                                                                                                                     CGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG                     <  NB501016:230:HFVJLAFXY:1:11211:15609:18065/147‑1 (MQ=60)
                                                                                                                                      GAAGAATAACGGAAACGCCATAAACCGGAAAATTTTCATAAATAGCGAAAACCAGCGACGTCGCCGACCCGTAACCTGTCGCATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATC                <  NB501016:230:HFVJLAFXY:2:21306:26676:20071/151‑1 (MQ=60)
                                                                                                                                      GAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATC                <  NB501016:230:HFVJLAFXY:2:21110:5718:11485/151‑1 (MQ=60)
                                                                                                                                       AAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATGAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAATATCATCTACATATCACAACGTGCGTGGAGGCCATC                >  NB501016:230:HFVJLAFXY:3:21506:7902:12208/1‑150 (MQ=60)
                                                                                                                                       AAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCA               <  NB501016:230:HFVJLAFXY:2:21105:15639:10653/151‑1 (MQ=60)
                                                                                                                                        AGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCA               >  NB501016:230:HFVJLAFXY:3:21504:1237:10222/1‑150 (MQ=60)
                                                                                                                                         GAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCAT                 <  NB501016:230:HFVJLAFXY:2:21111:25544:13533/147‑1 (MQ=60)
                                                                                                                                           AGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACC           <  NB501016:230:HFVJLAFXY:3:21502:11186:1366/151‑1 (MQ=60)
                                                                                                                                            GAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACC           >  NB501016:230:HFVJLAFXY:4:11401:13257:17495/1‑150 (MQ=60)
                                                                                                                                              ACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCTGATCACCGGAAAGGACCCGTAAAGTGATAATGATTA                                                  <  NB501016:230:HFVJLAFXY:1:21205:9724:2957/109‑1 (MQ=53)
                                                                                                                                               CGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCCTGGAGGCCATCAAACCACGT       >  NB501016:230:HFVJLAFXY:1:11204:14790:11616/1‑151 (MQ=60)
                                                                                                                                                 GAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAACCCACGTC      >  NB501016:230:HFVJLAFXY:3:21406:26435:9978/1‑150 (MQ=60)
                                                                                                                                                 GAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCA     <  NB501016:230:HFVJLAFXY:4:21405:22296:3046/151‑1 (MQ=60)
                                                                                                                                                 GAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTC      >  NB501016:230:HFVJLAFXY:1:21106:8524:19778/1‑150 (MQ=60)
                                                                                                                                                 GAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAG                      >  NB501016:230:HFVJLAFXY:2:11311:18196:17683/1‑134 (MQ=60)
                                                                                                                                                    ACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAAT  <  NB501016:230:HFVJLAFXY:2:21105:2795:10162/151‑1 (MQ=60)
                                                                                                                                                    ACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAAT  <  NB501016:230:HFVJLAFXY:4:11504:22295:10303/151‑1 (MQ=60)
                                                                                                                                                    ACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAAT  <  NB501016:230:HFVJLAFXY:1:21311:25446:9869/151‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAAT  >  NC_000913/1636354‑1636652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑