Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F15 I1 R1
|
9 |
36.2 |
1317874 |
96.5% |
1271748 |
132.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
758,061 |
G→T |
G125* (GGA→TGA) |
sdhB → |
succinate dehydrogenase, FeS subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 758,061 | 0 | G | T | 100.0%
| 168.4
/ NA
| 48 | G125* (GGA→TGA) | sdhB | succinate dehydrogenase, FeS subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (25/23); total (25/23) |
CCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGG > NC_000913/757918‑758200
|
ccccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACa > 5:2840/1‑148 (MQ=255)
cccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACaa > 1:58187/1‑148 (MQ=255)
cccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACaa > 2:157044/1‑148 (MQ=255)
ccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGa < 1:153073/144‑1 (MQ=255)
ccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGa > 2:153073/1‑144 (MQ=255)
ccGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACaaaa < 2:58187/149‑1 (MQ=255)
ggCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCg > 1:5165/1‑145 (MQ=255)
ggCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCg < 2:5165/145‑1 (MQ=255)
ggCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACa < 1:110277/149‑1 (MQ=255)
gaagaTTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGc < 6:2840/149‑1 (MQ=255)
ttGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCagag < 1:157044/149‑1 (MQ=255)
ttCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAgcgc < 2:151817/149‑1 (MQ=255)
ccAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTAc < 6:56021/121‑1 (MQ=255)
ccAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTAc > 5:56021/1‑121 (MQ=255)
gTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACggg > 1:169631/1‑149 (MQ=255)
ggTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCa < 7:34276/118‑1 (MQ=255)
ggTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCa > 8:34276/1‑118 (MQ=255)
ccGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTa > 6:168685/1‑149 (MQ=255)
cgcgATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAg > 3:92400/1‑105 (MQ=255)
cgcgATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAg < 4:92400/105‑1 (MQ=255)
gcgATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTAtt < 2:169631/149‑1 (MQ=255)
gATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCt > 3:118722/1‑134 (MQ=255)
gATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCt < 4:118722/134‑1 (MQ=255)
gtAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGAt < 2:63687/95‑1 (MQ=255)
gtAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGAt > 1:63687/1‑95 (MQ=255)
aTGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATgttgtt > 3:62129/1‑148 (MQ=255)
gggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCaa > 2:119694/1‑149 (MQ=255)
gggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCACCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCAtgt > 2:120383/1‑142 (MQ=255)
gggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCACCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCAtgt < 1:120383/142‑1 (MQ=255)
ggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGaaaaa < 7:114370/105‑1 (MQ=255)
ggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGaaaaa > 8:114370/1‑105 (MQ=255)
ggACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAAc > 3:138939/1‑149 (MQ=255)
aaTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTcgc < 5:132981/68‑1 (MQ=255)
aaTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTcgc > 6:132981/1‑68 (MQ=255)
aTTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCtt > 1:86828/1‑149 (MQ=255)
cgcAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCt > 2:52386/1‑149 (MQ=255)
atatGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCt < 1:52386/149‑1 (MQ=255)
tGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtggt > 1:43959/1‑149 (MQ=255)
gagaAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTctct < 4:34160/112‑1 (MQ=255)
gagaAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTctct > 3:34160/1‑112 (MQ=255)
agaAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtggtg < 7:73438/148‑1 (MQ=255)
agaAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCtggtg > 8:73438/1‑148 (MQ=255)
aCCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGt < 1:119694/147‑1 (MQ=255)
ttGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAAc > 6:526/1‑64 (MQ=255)
ttGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAAc < 5:526/64‑1 (MQ=255)
ttGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCgg < 4:62129/149‑1 (MQ=255)
gAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTAt < 6:147276/76‑1 (MQ=255)
gAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTAt > 5:147276/1‑76 (MQ=255)
|
CCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTTTATCGG > NC_000913/757918‑758200
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTT > NC_000913/757917‑758194
|
ACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAA > NB501016:228:HFK2FAFXY:3:11402:7622:3644/1‑150 (MQ=60)
CCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAA > NB501016:228:HFK2FAFXY:1:11210:6799:8543/1‑150 (MQ=60)
CCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGAC < NB501016:228:HFK2FAFXY:1:21210:21791:7171/146‑1 (MQ=60)
CGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGC > NB501016:228:HFK2FAFXY:1:11102:16111:17338/1‑147 (MQ=60)
GGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAG < NB501016:228:HFK2FAFXY:1:21105:10819:17072/151‑1 (MQ=60)
ATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGC < NB501016:228:HFK2FAFXY:1:21211:21225:17670/151‑1 (MQ=60)
GCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACA > NB501016:228:HFK2FAFXY:3:11512:8278:1171/1‑123 (MQ=60)
GGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGC > NB501016:228:HFK2FAFXY:1:21304:10057:14584/1‑151 (MQ=60)
CGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAG < NB501016:228:HFK2FAFXY:4:11504:4002:13561/120‑1 (MQ=60)
CCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGA > NB501016:228:HFK2FAFXY:2:21109:22802:4445/1‑107 (MQ=60)
CGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTG > NB501016:228:HFK2FAFXY:2:21210:15679:11059/1‑136 (MQ=60)
GGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATG > NB501016:228:HFK2FAFXY:1:11212:15371:9663/1‑97 (MQ=60)
GTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTT > NB501016:228:HFK2FAFXY:3:21607:13721:15527/1‑151 (MQ=60)
CATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTC > NB501016:228:HFK2FAFXY:2:11306:22554:18280/1‑150 (MQ=60)
TGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCACCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTT < NB501016:228:HFK2FAFXY:1:21109:18439:13339/144‑1 (MQ=60)
GGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACC > NB501016:228:HFK2FAFXY:2:21308:13228:2789/1‑151 (MQ=60)
GGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAAC < NB501016:228:HFK2FAFXY:4:21508:3771:7815/107‑1 (MQ=60)
CAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCG < NB501016:228:HFK2FAFXY:3:21508:19626:13616/70‑1 (MQ=60)
AATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTG > NB501016:228:HFK2FAFXY:1:11309:20028:3181/1‑151 (MQ=60)
AATATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTG < NB501016:228:HFK2FAFXY:1:11208:19611:4350/151‑1 (MQ=60)
ATGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTG > NB501016:228:HFK2FAFXY:1:11205:7064:2161/1‑151 (MQ=60)
TGAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGCCGGGCTGTATGAATGTATTCTCTG > NB501016:228:HFK2FAFXY:2:11205:17005:18887/1‑114 (MQ=60)
GAGAAAATTAAGCCTTACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGG < NB501016:228:HFK2FAFXY:4:11612:19531:10303/150‑1 (MQ=60)
TACCTGTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTT < NB501016:228:HFK2FAFXY:1:21109:3892:7922/149‑1 (MQ=60)
GTTGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACT < NB501016:228:HFK2FAFXY:3:11401:24307:5109/66‑1 (MQ=60)
TGAATAATTGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATG > NB501016:228:HFK2FAFXY:3:21602:17384:18245/1‑78 (MQ=60)
|
ACCCCGATTTCGGCACTCAACCAGCCGGGCAAGAAGATTGTGATTCGCCCGCTGCCAGGTTTACCGGTGATCCGCGATTTGGTGGTAGACATGGGACAATTCTATGCGCAATATGAGAAAATTAAGCCTTACCTGTTGAATAATGGACAAAATCCGCCAGCTCGCGAGCATTTACAGATGCCAGAGCAGCGCGAAAAACTCGACGGGCTGTATGAATGTATTCTCTGCGCATGTTGTTCAACCTCTTGTCCGTCTTTCTGGTGGAATCCCGATAAGTT > NC_000913/757917‑758194
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
