Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F36 I1 R1 9 107.0 3793126 97.8% 3709677 139.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTG  >  NC_000913/755195‑755473
                                                                                                                                             |                                                                                                                                         
AAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAG                                                                                                                                  <  NB501016:230:HFVJLAFXY:3:21505:22624:3395/151‑1 (MQ=60)
               AATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTC                                                                                                                  >  NB501016:230:HFVJLAFXY:4:11501:10522:9142/1‑151 (MQ=60)
                   TGGACCTACAGACCATCCGGTTCCCCAACACGGCGATAGCGTCCATTATCCATAGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTGTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCG                                                                                                              >  NB501016:230:HFVJLAFXY:1:21212:19777:6686/1‑151 (MQ=60)
                       CCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCGGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATT                                                                                                           >  NB501016:230:HFVJLAFXY:3:11501:12500:6079/1‑151 (MQ=60)
                       CCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCATCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATT                                                                                                           >  NB501016:230:HFVJLAFXY:1:21103:12567:4999/1‑151 (MQ=60)
                          ACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTA                                                                                                          <  NB501016:230:HFVJLAFXY:3:21402:19708:4514/149‑1 (MQ=60)
                               CCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGC                                                                                                  >  NB501016:230:HFVJLAFXY:3:11606:22407:8801/1‑151 (MQ=60)
                               CCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTA                                                                                                          <  NB501016:230:HFVJLAFXY:3:11401:17543:6614/143‑1 (MQ=60)
                                    CGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCT                                                                                              >  NB501016:230:HFVJLAFXY:3:21509:13612:18037/1‑151 (MQ=60)
                                         CCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTC                                                                                         >  NB501016:230:HFVJLAFXY:1:21302:1399:6607/1‑150 (MQ=60)
                                          CCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTC                                                                                         >  NB501016:230:HFVJLAFXY:3:11503:22683:7996/1‑150 (MQ=60)
                                          CCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCA                                                                                        >  NB501016:230:HFVJLAFXY:2:11312:3253:17908/1‑150 (MQ=60)
                                           CCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCA                                                                                        >  NB501016:230:HFVJLAFXY:2:21303:23365:20093/1‑150 (MQ=60)
                                                ACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGTCTTCTGGGTAGCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTAT                                                                                 <  NB501016:230:HFVJLAFXY:3:11510:24454:7482/151‑1 (MQ=60)
                                                       TAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCA                                                                                                    <  NB501016:230:HFVJLAFXY:4:11512:25699:16608/125‑1 (MQ=60)
                                                          CGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGT                                                                                                                                >  NB501016:230:HFVJLAFXY:4:11603:11223:8743/1‑94 (MQ=60)
                                                              CATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTCCC                                                                                                                                         <  NB501016:230:HFVJLAFXY:3:11608:25309:2930/82‑1 (MQ=60)
                                                               ATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGCGGGGCATCCT                                                                  >  NB501016:230:HFVJLAFXY:1:21108:10156:17966/1‑151 (MQ=60)
                                                                  CTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTAC                                                               >  NB501016:230:HFVJLAFXY:4:11412:18189:6286/1‑151 (MQ=60)
                                                                   TCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCG                                                                                           <  NB501016:230:HFVJLAFXY:1:11203:3884:16748/123‑1 (MQ=60)
                                                                    CCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCG                                                             >  NB501016:230:HFVJLAFXY:2:21102:16998:4517/1‑151 (MQ=60)
                                                                    CCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTA                                                                                  >  NB501016:230:HFVJLAFXY:1:11207:18483:13487/1‑130 (MQ=60)
                                                                    CCATCGCGTTTCCGGTGTGATCACCTTTGGTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTAC                                                               <  NB501016:230:HFVJLAFXY:3:11609:4281:18583/149‑1 (MQ=60)
                                                                     CATCGCGTTTGCGGTGGGATCACCTTTGTTGCAGTGTGCATCCTGCTGTGGCTTCTGTGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCTATTTTATCATGTGGGGCATCCTTACCG                                                             <  NB501016:230:HFVJLAFXY:1:11108:26392:1869/151‑1 (MQ=60)
                                                                     CATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCA                                                                               <  NB501016:230:HFVJLAFXY:2:21111:21601:13712/133‑1 (MQ=60)
                                                                     CATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCG                                                                                           <  NB501016:230:HFVJLAFXY:4:21508:18052:1527/120‑1 (MQ=60)
                                                                     CATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCT                                                                                                 <  NB501016:230:HFVJLAFXY:2:21107:8124:9013/114‑1 (MQ=60)
                                                                      ATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGC                                                                                                                     >  NB501016:230:HFVJLAFXY:4:11505:20915:17765/1‑93 (MQ=60)
                                                                                    GTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGT                                             >  NB501016:230:HFVJLAFXY:4:21603:15419:13758/1‑151 (MQ=60)
                                                                                      GATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCG                                           >  NB501016:230:HFVJLAFXY:3:11401:24238:7886/1‑151 (MQ=60)
                                                                                      GATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCG                                           <  NB501016:230:HFVJLAFXY:2:11301:26109:7998/151‑1 (MQ=60)
                                                                                         CACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCA                                                <  NB501016:230:HFVJLAFXY:2:21106:12459:11486/143‑1 (MQ=60)
                                                                                            CTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATC                                                                                <  NB501016:230:HFVJLAFXY:2:21102:13451:7765/109‑1 (MQ=60)
                                                                                            CTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGCGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATTGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTCGCGTATCACGTCGTagtat                                     >  NB501016:230:HFVJLAFXY:4:21507:13869:2338/1‑146 (MQ=60)
                                                                                              TTGTTGCCGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCTCTATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAG                                     <  NB501016:230:HFVJLAFXY:2:11207:8009:18657/150‑1 (MQ=60)
                                                                                               TGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGG                                    <  NB501016:230:HFVJLAFXY:1:21105:11128:17088/150‑1 (MQ=60)
                                                                                               TGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAG                                                                                                                                  <  NB501016:230:HFVJLAFXY:1:21101:14954:17731/56‑1 (MQ=60)
                                                                                                GTTGCAGTGGGCATACTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCGTGCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGT                                   >  NB501016:230:HFVJLAFXY:3:11607:17291:19493/1‑150 (MQ=60)
                                                                                                  TGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTC                               >  NB501016:230:HFVJLAFXY:1:21103:21000:3158/1‑151 (MQ=60)
                                                                                                   GCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCG                              >  NB501016:230:HFVJLAFXY:2:11101:4387:9293/1‑151 (MQ=60)
                                                                                                        GGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCAC                          >  NB501016:230:HFVJLAFXY:3:21510:1893:15642/1‑151 (MQ=60)
                                                                                                            ATCCTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGAGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATACTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTAGCCACATGAT                     >  NB501016:230:HFVJLAFXY:1:11101:1405:14277/1‑151 (MQ=60)
                                                                                                              CCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATG                    <  NB501016:230:HFVJLAFXY:4:11501:4122:9062/151‑1 (MQ=60)
                                                                                                               CTGCTGTGGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCC                            <  NB501016:230:HFVJLAFXY:4:11507:21143:6221/141‑1 (MQ=60)
                                                                                                                  CTGTGGCTTATGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTACGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGG                >  NB501016:230:HFVJLAFXY:3:11507:2271:14452/1‑150 (MQ=60)
                                                                                                                      GGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGG                                    >  NB501016:230:HFVJLAFXY:4:11412:23031:15873/1‑126 (MQ=60)
                                                                                                                      GGCTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGG                                    >  NB501016:230:HFVJLAFXY:4:21411:4580:7599/1‑126 (MQ=60)
                                                                                                                        CTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCTGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTG          >  NB501016:230:HFVJLAFXY:1:21310:15195:10828/1‑151 (MQ=60)
                                                                                                                        CTTCTGGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGG         >  NB501016:230:HFVJLAFXY:3:11604:9955:2049/1‑151 (MQ=60)
                                                                                                                            TGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTGCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTG          <  NB501016:230:HFVJLAFXY:3:11505:4038:12924/147‑1 (MQ=60)
                                                                                                                             GGGTACCAGCCTCTC‑TCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATC    <  NB501016:230:HFVJLAFXY:3:21412:24972:11140/151‑1 (MQ=60)
                                                                                                                                      CCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTG  >  NB501016:230:HFVJLAFXY:3:21608:24940:4859/1‑145 (MQ=60)
                                                                                                                                             |                                                                                                                                         
AAACAAAGACCTGTTAATCTGGACCTACAGACCATCCGGTTCCCCATCACGGCGATAGCGTCCATTCTCCATCGCGTTTCCGGTGTGATCACCTTTGTTGCAGTGGGCATCCTGCTGTGGCTTCTGGGTACCAGCCTCTCTTCCCCTGAAGGTTTCGAGCAAGCTTCCGCGATTATGGGCAGCTTCTTCGTCAAATTTATCATGTGGGGCATCCTTACCGCTCTGGCGTATCACGTCGTCGTAGGTATTCGCCACATGATGATGGATTTTGGCTATCTG  >  NC_000913/755195‑755473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑