Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F8 I1 R1 10 32.8 1234644 96.3% 1188962 128.0

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCAGCGTTTTACCGCTATAAGTATTCCCTGCATTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACA  >  NC_000913/1595924‑1596188
                                                                                                                                   |                                                                                                                                     
GACCAGCGTTTTACCGCTATAAGTATTCCCTGCATTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGGATGATTAAAGACAAAAACG                                                                                                                    <  NB501016:228:HFK2FAFXY:2:21212:12669:1445/151‑1 (MQ=60)
                    AAGTATTCCCTGCATTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTTGTAGCCGGCATCACTGTTATTGGGATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTC                                                                                                >  NB501016:228:HFK2FAFXY:1:21306:26079:1773/1‑151 (MQ=60)
                                      actggagttcagacgtgtgctccttccgatcTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGT                                                                              <  NB501016:228:HFK2FAFXY:1:11107:13329:17171/120‑1 (MQ=60)
                                         CCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGTGAT                                                                           <  NB501016:228:HFK2FAFXY:3:21610:19155:11563/151‑1 (MQ=60)
                                                  CTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGGATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGTATTGGTGATAAATCCGGC                                                                  >  NB501016:228:HFK2FAFXY:4:11405:5129:4443/1‑151 (MQ=60)
                                                                              ATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGGATGATT                                                                                                                                 >  NB501016:228:HFK2FAFXY:4:21611:26471:15291/1‑60 (MQ=60)
                                                                                              AGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGGATGATTAAAGAC                                                                                                                           <  NB501016:228:HFK2FAFXY:2:21206:2968:18266/50‑1 (MQ=60)
                                                                                                 ATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGAGAGCAG                                        >  NB501016:228:HFK2FAFXY:4:11511:1837:10661/1‑130 (MQ=60)
                                                                                                              AGCCGGCATCACTGTTATTGGGATGATTAAAGACAAAAACGCCTTCGCCAAGACCACACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAG                <  NB501016:228:HFK2FAFXY:1:21207:8091:17752/141‑1 (MQ=60)
                                                                                                                 CGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAAC   <  NB501016:228:HFK2FAFXY:4:11607:25665:6128/151‑1 (MQ=60)
                                                                                                                  GGGATCACTGTTATTGGGATGATTAAAGACAAAAACGCCTTCTCCAAGACCAAACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACA  <  NB501016:228:HFK2FAFXY:1:21201:21357:14937/151‑1 (MQ=60)
                                                                                                                       CACTTTTATTGGTATGTTTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTGGCATTGGTGATAAATCCGGCATCTGCCGCCGCCGCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAG             <  NB501016:228:HFK2FAFXY:4:21409:17368:2680/135‑1 (MQ=60)
                                                                                                                              ATTGGGATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAG                                        >  NB501016:228:HFK2FAFXY:1:11306:7554:14368/1‑101 (MQ=60)
                                                                                                                                   GATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCG                                               <  NB501016:228:HFK2FAFXY:3:11602:24442:15712/89‑1 (MQ=60)
                                                                                                                                   |                                                                                                                                     
GACCAGCGTTTTACCGCTATAAGTATTCCCTGCATTGAACACCGTATGGCCTGCATCATGGATCACTTTTCCGTCTTTATCGTCACCTGTAATCAGCATATCGACCTGGTAGCCGGCATCACTGTTATTGGTATGATTAAAGACAAAAACGCCTTCGCCAAGACCAAACTCCACTTTCGTCGCATTGGTGATAAATCCGGCATCTGCCGCCGCCTCACCGTGAGCAGCACCAATGTTTAGCGTTCCTACAGCAGGCTCAAAAACA  >  NC_000913/1595924‑1596188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑