Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F36 I1 R1	14	89.5	2915230	98.1%	2859840	144.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	762,928	1 bp→GG	intergenic (+189/‑86)	sucB → / → sucC	dihydrolipoyltranssuccinase/succinyl‑CoA synthetase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	762,927	1	.	G	96.4%	287.6 / 3.4	83	intergenic (+188/‑87)	sucB/sucC	dihydrolipoyltranssuccinase/succinyl‑CoA synthetase, beta subunit
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (45/35); total (46/37)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01
*	NC_000913	762,928	0	C	G	100.0%	297.7 / NA	83	intergenic (+189/‑86)	sucB/sucC	dihydrolipoyltranssuccinase/succinyl‑CoA synthetase, beta subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (46/37); total (46/37)

CCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑C‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGTGG  >  NC_000913/762785‑763077

|||

ccTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑g‑gg‑ggt                                                                                                                                                   <  7:193690/149‑3 (MQ=255)

ccTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑g‑gg‑ggt                                                                                                                                                   >  7:60043/1‑147 (MQ=255)

   tctcCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑g‑gg‑ggtgac                                                                                                                                                >  4:235611/1‑144 (MQ=255)

    ctcCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑g‑gg‑ggtgac                                                                                                                                                <  3:235611/148‑6 (MQ=255)

        ggCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGAc                                                                                                                                             >  2:351351/1‑148 (MQ=255)

        ggCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGAc                                                                                                                                             >  2:346407/1‑148 (MQ=255)

         gCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACaa                                                                                                                                           >  2:357783/1‑149 (MQ=255)

         gCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACaa                                                                                                                                           >  2:267121/1‑149 (MQ=255)

            aTTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGc                                                                                                                                        >  2:156518/1‑149 (MQ=255)

             ttGAAGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGcc                                                                                                                                       <  6:165741/149‑1 (MQ=255)

               gAAGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGccc                                                                                                                                      <  8:25090/148‑1 (MQ=255)

                aaGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCg                                                                                                                                     >  3:199159/1‑148 (MQ=255)

                 aGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCgg                                                                                                                                    >  3:104801/1‑148 (MQ=255)

                   ccTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAg                                                                                                                                 >  3:7183/1‑149 (MQ=255)

                            aCGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGaa                                                                                                                        <  7:221210/149‑1 (MQ=255)

                             cGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGaaa                                                                                                                       <  1:267121/149‑1 (MQ=255)

                               cTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAata                                                                                                                     >  2:329819/1‑149 (MQ=255)

                                  aCGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTc                                                                                                                  <  1:351351/149‑1 (MQ=255)

                                  aCGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑G‑GGTGTGACCGACAATGCCCGGAAGCGATACGAAATATTc                                                                                                                  <  1:346407/149‑1 (MQ=255)

                                   cgcgTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                                                                                 <  3:91679/149‑1 (MQ=255)

                                   cgcgTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                                                                                 <  8:60043/149‑1 (MQ=255)

                                            tCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGt                                                                                                        <  5:90617/149‑1 (MQ=255)

                                               ggCCTACGGGTCCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGttt                                                                                                      >  3:18416/1‑148 (MQ=255)

                                               ggCATACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTa                                                                                                     <  1:357783/149‑1 (MQ=255)

                                                gCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGTTGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTa                                                                                                     >  5:161343/1‑148 (MQ=255)

                                                gCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTTTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTaa                                                                                                    <  4:108053/149‑1 (MQ=255)

                                                 ccTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCGACGGTTTaa                                                                                                    >  6:334250/1‑148 (MQ=255)

                                                  cTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTaaaa                                                                                                  <  5:192485/149‑1 (MQ=255)

                                                  cTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTaaaa                                                                                                  <  1:156518/149‑1 (MQ=255)

                                                     cGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGa                                                                                                >  4:334085/1‑148 (MQ=255)

                                                      gggACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATa                                                                                              <  8:274967/149‑1 (MQ=255)

                                                        gACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATAtt                                                                                                                   <  3:156451/126‑1 (MQ=255)

                                                        gACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATAtt                                                                                                                   >  4:156451/1‑126 (MQ=255)

                                                          ccaccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGa                                                                                          <  6:213538/149‑1 (MQ=255)

                                                           caccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGAt                                                                                         <  1:272789/149‑1 (MQ=255)

                                                             ccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTa                                                                                       >  6:244574/1‑149 (MQ=255)

                                                              ctaTGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTTACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTAc                                                                                      <  6:350564/147‑1 (MQ=255)

                                                                aTGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTg                                                                                    <  1:9597/149‑1 (MQ=255)

                                                                  gTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTg                                                                                    >  3:163839/1‑147 (MQ=255)

                                                                   tAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGaa                                                                                  <  1:148708/148‑1 (MQ=255)

                                                                    ttgTCGGATGCGGCGCTTGCGCCTTATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAg                                                                                 >  8:41400/3‑148 (MQ=255)

                                                                     ggTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGa                                                                               >  1:92337/1‑149 (MQ=255)

                                                                      gTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGAt                                                                              >  6:284853/1‑149 (MQ=255)

                                                                       tCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAATAGATAACGATTACTGAAGGATg                                                                             >  7:133741/1‑149 (MQ=255)

                                                                          gATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGa                                                                           >  1:206190/1‑148 (MQ=255)

                                                                          gATAACGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGt                                                                                                        <  1:100691/119‑1 (MQ=255)

                                                                           aTAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGa                                                                           >  2:107369/1‑147 (MQ=255)

                                                                            tAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGAt                                                                              >  1:34897/1‑143 (MQ=255)

                                                                            tAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGAt                                                                              <  2:34897/143‑1 (MQ=255)

                                                                              aGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGaa                                                                      <  5:171136/149‑1 (MQ=255)

                                                                               ggCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAac                                                                     <  4:104801/149‑1 (MQ=255)

                                                                                gcgcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTa                                                                                                     <  8:294482/116‑1 (MQ=255)

                                                                                gcgcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCGAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTa                                                                                                     >  7:294482/1‑116 (MQ=255)

                                                                                  gcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATaa                                                                                             >  3:22694/1‑122 (MQ=255)

                                                                                  gcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATaa                                                                                             <  4:22694/122‑1 (MQ=255)

                                                                                  gcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAAcaca                                                                  >  8:179684/1‑149 (MQ=255)

                                                                                  gcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAAcaca                                                                  >  2:73846/1‑149 (MQ=255)

                                                                                   cttgcgcCTTATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACAt                                                                 <  7:41400/146‑1 (MQ=255)

                                                                                      gcgcCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGaa                                                              <  4:7183/149‑1 (MQ=255)

                                                                                        gccgcATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACt                                                            >  4:304896/1‑149 (MQ=255)

                                                                                        gccgcATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACt                                                            >  4:266179/1‑149 (MQ=255)

                                                                                            cATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACa                                                        >  5:284064/1‑149 (MQ=255)

                                                                                             aTCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACAt                                                       <  2:220174/149‑1 (MQ=255)

                                                                                                  aCAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACAt                                                                 <  7:110581/134‑1 (MQ=255)

                                                                                                  aCAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACAt                                                                 >  8:110581/1‑134 (MQ=255)

                                                                                                     aGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑Ca                                              <  1:107369/149‑1 (MQ=255)

                                                                                                      gCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAg                                             >  7:10897/1‑149 (MQ=255)

                                                                                                          tGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCTATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTCCTGAAGGATTGACAGAACACATGAACTTTCATTAATCT‑AAGGCa                                          >  6:336462/1‑148 (MQ=255)

                                                                                                             cTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCaaaa                                       >  5:253765/1‑148 (MQ=255)

                                                                                                             cTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCAGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAaac                                      <  4:199159/149‑1 (MQ=255)

                                                                                                               gATGTGACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAaacaa                                    >  8:88350/1‑149 (MQ=255)

                                                                                                                 tgtgACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGCCCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATATCGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATACATGCaaatgat                                    >  8:7958/1‑144 (MQ=255)

                                                                                                                   tgACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACttt                                <  4:78267/149‑1 (MQ=255)

                                                                                                                   tgACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACttt                                <  7:88350/149‑1 (MQ=255)

                                                                                                                   tgACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACttt                                >  3:46709/1‑149 (MQ=255)

                                                                                                                    gACGTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACtttt                               >  7:19588/1‑149 (MQ=255)

                                                                                                                       gTTTAACGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGc                            >  2:54847/1‑149 (MQ=255)

                                                                                                                            aCGTGTCTTATCAGGCCTA‑G‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTa                      >  8:22274/1‑149 (MQ=255)

                                                                                                                             cGTGTCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTa                      >  6:309760/1‑149 (MQ=255)

                                                                                                                              gtgtCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTAt                     <  3:334085/149‑1 (MQ=255)

                                                                                                                                gtCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATgg                   >  6:77967/1‑149 (MQ=255)

                                                                                                                                gtCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATgg                   >  2:165688/1‑149 (MQ=255)

                                                                                                                                 tCTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATgg                   <  6:284064/148‑1 (MQ=255)

                                                                                                                                  cTTATCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCt                 >  3:160720/1‑149 (MQ=255)

                                                                                                                                    tATCAGGCCTAGG‑GG‑GTGACCTACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CCGGCAACACAACTTTTTGCCCGCTATGGCTTa               >  4:290816/1‑149 (MQ=255)

                                                                                                                                      tCAGGCCTAGG‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTAc              >  2:4369/1‑148 (MQ=255)

                                                                                                                                        aGGCCTA‑GGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCa            <  1:73846/148‑1 (MQ=255)

                                                                                                                                            cctagggg‑gTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCAc        <  5:284853/143‑1 (MQ=255)

                                                                                                                                             ctagggg‑gTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCAcc       <  3:304896/144‑1 (MQ=255)

                                                                                                                                                gggg‑gTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGt    <  3:266179/147‑1 (MQ=255)

                                                                                                                                                gggg‑gTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGt    >  3:17686/3‑149 (MQ=255)

                                                                                                                                                 ggg‑gTGACCGACAATGCCCGGAGGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGTg   >  4:21103/2‑149 (MQ=255)

                                                                                                                                                  gg‑gTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGTgg  <  6:161343/149‑1 (MQ=255)

|||

CCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑C‑GG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATAT‑CAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAGCACCGGTGG  >  NC_000913/762785‑763077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A