Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F30 I1 R1 17 32.7 1267382 95.3% 1207815 125.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGATGTCAGCACCCTGAAAGAGCTGGCGCGCCGCTGGAAGCCGGAAATTCTGGATGGTTTTACCAAGCAGGGGACGCATC  >  NC_000913/4391799‑4392078
                                                                                                                                   |                                                                                                                                                    
CATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATTGGTCAGGACCGTCGTTTCC                                                                                                                                   <  NB501016:228:HFK2FAFXY:4:11511:4123:13021/151‑1 (MQ=60)
CATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATTGGTCAGGACCGTCGTTTCC                                                                                                                                   >  NB501016:228:HFK2FAFXY:3:11406:26652:10842/1‑151 (MQ=60)
    tttttttttcaagcagaagacggcatacgagatctatcgctgtgatggagttcagacgtgtgctcttccgatctTTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATTGGTCAGGACCGTCGTTTCCTG                                                                                                                                 <  NB501016:228:HFK2FAFXY:4:21601:18363:19104/75‑1 (MQ=60)
    CCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATTGGTCAGGACCGTCGTTTCCTGTT                                                                                                                               <  NB501016:228:HFK2FAFXY:1:11111:9801:4363/151‑1 (MQ=60)
                  GTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGG                                                                                                                 <  NB501016:228:HFK2FAFXY:2:21306:13556:9507/151‑1 (MQ=60)
                    AGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACAT                                                                                                                      >  NB501016:228:HFK2FAFXY:2:11312:21437:16591/1‑144 (MQ=60)
                            TGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGG                                                                                                                                                    >  NB501016:228:HFK2FAFXY:1:21211:13822:9737/1‑106 (MQ=60)
                             GAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGG                                                                                                                                               <  NB501016:228:HFK2FAFXY:1:21103:9360:17007/110‑1 (MQ=60)
                                    AGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATTGGTCAGGACCGTCGagatcggaagagcacaacgtctgaactccagtcacagcgat                                                                                               >  NB501016:228:HFK2FAFXY:1:21306:1941:8648/1‑110 (MQ=60)
                                        CGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATTGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTC                                                                                           >  NB501016:228:HFK2FAFXY:4:21510:15939:16458/1‑151 (MQ=60)
                                        CGATGGGCGATCGGGAAGCTGAACTGACAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATTGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACT                                                                                            >  NB501016:228:HFK2FAFXY:4:11408:13580:4033/1‑150 (MQ=60)
                                                               CTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATTGG                                                                                                                                                    <  NB501016:228:HFK2FAFXY:2:21204:3063:5958/71‑1 (MQ=60)
                                                                                       CAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTagatcggaaagagcacacgtctgaactccagtcacagcgatagatgtcgtatgccggcttctgcttgaaaaaaaaaaggggggg                                            >  NB501016:228:HFK2FAFXY:2:11105:4024:15796/1‑67 (MQ=60)
                                                                                                           ATCGCCGATTTGCGGTAACAGCATTGGTCAGGACCGTCGTTTCCTGTTTACATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGATGTCAGCACCCTGAAAGAGCTGGCGCGCCGCTGGAAGCCGGAAATTCTGGATGGT                        <  NB501016:228:HFK2FAFXY:3:21605:16625:12343/151‑1 (MQ=60)
                                                                                                              GCCGATTTGCGGTAACAGCATTGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGATGTCAGCACCCTGAAAGAGCTGGCGCGCCGCTGGAAGCCGGAAATTCTGGATGGTTTT                     <  NB501016:228:HFK2FAFXY:3:21611:15116:19648/151‑1 (MQ=60)
                                                                                                                          TAACAGCATCGGTCAGGACCGTCGTTTGCTGTTTAAATACAAGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGAT                                                                              >  NB501016:228:HFK2FAFXY:2:21111:25938:19102/1‑82 (MQ=60)
                                                                                                                                 ATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGATGTCAGCACCCTGAAAGAGCTGGCGCGCCGCTGGAAGCCGGAAATTCTGGATGGTTTTACCAAGCAGGGGACGCATC  <  NB501016:228:HFK2FAFXY:1:21312:23437:3928/151‑1 (MQ=60)
                                                                                                                                   |                                                                                                                                                    
CATACCGCCAGCGGGCTGGTAGAGCGCGTGAAAGCGAGCACGATGGGCGATCGGGAAGCTGAACTGGCAACGCTCGAATTTTTAAAACAGTGGGTGCCTGCGGGAAAATCGCCGATTTGCGGTAACAGCATCGGTCAGGACCGTCGTTTCCTGTTTAAATACATGCCGGAGCTGGAAGCCTACTTCCACTACCGTTATCTCGATGTCAGCACCCTGAAAGAGCTGGCGCGCCGCTGGAAGCCGGAAATTCTGGATGGTTTTACCAAGCAGGGGACGCATC  >  NC_000913/4391799‑4392078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑