Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
46.7 |
5280542 |
98.1% |
5180211 |
47.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_017635 |
2,142,906 |
T→C |
K147R (AAG→AGG) |
uvrC ← |
excinuclease ABC subunit UvrC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_017635 | 2,142,906 | 0 | T | C | 82.2%
| 269.6
/ 48.9
| 101 | K147R (AAG→AGG) | uvrC | excinuclease ABC subunit UvrC |
| Reads supporting (aligned to +/- strand): ref base T (5/13); new base C (37/46); total (42/59) |
| Fisher's exact test for biased strand distribution p-value = 2.91e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 |
Only 100 of 103 total aligned reads displayed.
AGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACGGCATAGCCA > NC_017635/2142859‑2142944
|
agcgaTTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCt < 2:523511/48‑1 (MQ=255)
gaTTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCtttt < 2:1694289/48‑1 (MQ=255)
ttGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGc < 2:1941857/48‑1 (MQ=255)
gCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCa < 1:1140597/47‑1 (MQ=255)
gCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCa < 1:396275/47‑1 (MQ=255)
cGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAg < 1:93174/47‑1 (MQ=255)
tAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAgc > 2:2064998/1‑48 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 1:1401160‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc > 1:1034327‑M1/34‑48 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc > 1:1405956‑M1/34‑48 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc > 1:1526378‑M1/34‑48 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 1:515503‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc > 1:31639‑M1/34‑48 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 1:2598654‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 1:1790320‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc > 1:2595569‑M1/34‑48 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 1:1868888‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 1:2518113‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 1:1318185‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 2:442213‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 1:1305054‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 2:1140819‑M1/15‑1 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc > 2:2028870‑M1/34‑48 (MQ=255)
accagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgc < 2:2270074‑M1/15‑1 (MQ=255)
accagaagcagcatcagggacgacattagaaatATCCTTTGCAGTAgc < 1:563961‑M1/15‑1 (MQ=255)
ccagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcg < 1:463894‑M1/16‑1 (MQ=255)
ccagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcg < 1:2544229‑M1/16‑1 (MQ=255)
ccagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcg > 1:1126429‑M1/33‑48 (MQ=255)
ccagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcg < 1:1776994‑M1/16‑1 (MQ=255)
ccagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcg < 1:2333252‑M1/16‑1 (MQ=255)
ccagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcg < 1:1689519‑M1/16‑1 (MQ=255)
ccagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcg > 1:708569‑M1/33‑48 (MQ=255)
ccagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcg > 2:1771130‑M1/33‑48 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc < 2:854656‑M1/17‑1 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc < 1:2079250‑M1/17‑1 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc > 2:555024‑M1/32‑48 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc < 2:2479114‑M1/17‑1 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc > 2:2245325‑M1/32‑48 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc > 1:677044‑M1/32‑48 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc > 1:1653419‑M1/32‑48 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc > 1:1480380‑M1/32‑48 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc > 1:1094626‑M1/32‑48 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc < 1:1318228‑M1/17‑1 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc < 1:1953925‑M1/17‑1 (MQ=255)
cagaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc > 1:1602244‑M1/32‑48 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc < 1:435457‑M1/17‑1 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc < 1:685318‑M1/17‑1 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc < 1:1144680‑M1/17‑1 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAgcgc < 2:1455904‑M1/17‑1 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc > 1:2066332‑M1/31‑48 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc > 1:371420‑M1/31‑48 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc > 1:464000‑M1/31‑48 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc > 1:307744‑M1/31‑48 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc > 1:2503582‑M1/31‑48 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc > 1:983336‑M1/31‑48 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc > 1:902532‑M1/31‑48 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc > 1:1985044‑M1/31‑48 (MQ=255)
agaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc > 1:2072706‑M1/31‑48 (MQ=255)
acacTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAgcgc > 2:1783234/1‑47 (MQ=255)
acacTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAgcgc > 2:1877247/1‑47 (MQ=255)
gaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc < 1:1919350‑M1/18‑1 (MQ=255)
gaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGcc < 1:277742‑M1/18‑1 (MQ=255)
gaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCCa > 1:954788‑M1/30‑48 (MQ=255)
gaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCCa > 1:1313382‑M1/30‑48 (MQ=255)
gaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCCa > 1:176436‑M1/30‑48 (MQ=255)
gaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCCa > 1:2427090‑M1/30‑48 (MQ=255)
gaagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCCa > 1:2029488‑M1/30‑48 (MQ=255)
gaagcagaatcagtgacgacattagaaatATCCTTTGCAGTAGCGCCa > 1:778833‑M1/30‑48 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:1245860‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 2:2176224‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 2:2178361‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa > 2:2315152‑M1/29‑47 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 2:1097782‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 2:433077‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:1087890‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa > 1:1516428‑M1/29‑47 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:1606015‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:1609438‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:49288‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:1695257‑M1/20‑2 (MQ=255)
aagcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:2511478‑M1/20‑2 (MQ=255)
cTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCaga < 1:2500396/48‑1 (MQ=255)
agcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGccaat > 1:1092156‑M1/28‑46 (MQ=255)
agcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGccaat > 1:2259913‑M1/28‑46 (MQ=255)
agcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:1992430‑M1/20‑2 (MQ=255)
agcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:871735‑M1/20‑2 (MQ=255)
agcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:1135388‑M1/20‑2 (MQ=255)
agcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:29253‑M1/20‑2 (MQ=255)
agcagcatcagtgacgacattagaaatATCCTTTGCAGTAGCGCcaa < 1:308383‑M1/20‑2 (MQ=255)
agcagcagcagtgacgacattagaaatATCCTTTGCAGTAGCGccaat > 1:1332990‑M1/28‑46 (MQ=255)
ttttCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGAGtt < 1:1325494/48‑1 (MQ=255)
gCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCAc < 1:1796684/47‑1 (MQ=255)
tGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACg > 1:1169214/1‑48 (MQ=255)
gCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACGGc < 2:2373867/48‑1 (MQ=255)
aaTGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACGGCATa < 1:1204030/48‑1 (MQ=255)
aaTGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACGGCATa < 1:2494667/48‑1 (MQ=255)
aaTGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACGGCATa < 2:571054/48‑1 (MQ=255)
aTGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACGGCATAg < 1:1109572/48‑1 (MQ=255)
tGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACGGCATAGc > 1:1722449/1‑48 (MQ=255)
gggAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACGGCATAGCCa < 2:1246114/48‑1 (MQ=255)
|
AGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGAGTTTCACGTACGGCATAGCCA > NC_017635/2142859‑2142944
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
