Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
46.7 |
5280542 |
98.1% |
5180211 |
47.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_017635 |
2,322,732 |
T→C |
intergenic (‑40/+60) |
ECW_RS11650 ← / ← ECW_RS11655 |
head completion/stabilization protein/terminase endonuclease subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_017635 | 2,322,732 | 0 | T | C | 100.0%
| 94.4
/ NA
| 36 | intergenic (‑40/+60) | ECW_RS11650/ECW_RS11655 | head completion/stabilization protein/terminase endonuclease subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/15); total (21/15) |
CATCATGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGTGTCAGGTGATTCACCGTCACTGACCGGCGTGCCGCCCTGGCGC > NC_017635/2322687‑2322775
|
catcatGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAgcgt > 1:1099140/1‑48 (MQ=255)
catcatGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAgcgt > 2:1696692/1‑48 (MQ=255)
tcatGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCa < 1:952202/48‑1 (MQ=255)
catGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAg > 1:1179824/1‑48 (MQ=255)
aCTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGa < 1:1839959/48‑1 (MQ=255)
cTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGAt > 1:2086976/1‑48 (MQ=255)
cTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGAt > 2:492344/1‑48 (MQ=255)
cTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGAt > 2:409839/1‑48 (MQ=255)
tACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGAtt > 1:18702/1‑48 (MQ=255)
tACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGAtt > 2:830022/1‑48 (MQ=255)
ccTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTc < 1:1567258/47‑1 (MQ=255)
ccTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCa < 2:2265541/48‑1 (MQ=255)
ctctGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCAc < 2:839517/48‑1 (MQ=255)
ctctGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCAc > 2:1687792/1‑48 (MQ=255)
ctctGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCAc > 1:800604/1‑48 (MQ=255)
ctctGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCAc < 1:473538/48‑1 (MQ=255)
tGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCAcc < 2:1514182/46‑1 (MQ=255)
tGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCAcc < 2:1874585/46‑1 (MQ=255)
gAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCAccct > 2:2508710/1‑45 (MQ=11)
aaaaGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTc < 1:910531/47‑1 (MQ=11)
aaaaGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCa < 1:2279796/48‑1 (MQ=11)
aaaGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCAc > 2:2049858/1‑48 (MQ=11)
gTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCACTGa < 1:701589/48‑1 (MQ=11)
gTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCACTGa > 2:230051/1‑48 (MQ=11)
gTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCACTGa < 2:210158/48‑1 (MQ=11)
gTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCACTGa < 1:456607/48‑1 (MQ=11)
tGGACGCCGGTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTgc > 2:1978709/1‑48 (MQ=11)
aCGCCGGTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTgccgc > 1:2488885/1‑48 (MQ=11)
cGCCGGTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTgccgcc < 2:153596/48‑1 (MQ=11)
gCCGGTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGccc < 2:2614180/48‑1 (MQ=11)
tACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGgcg > 1:1294654/1‑48 (MQ=11)
tACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGgcg > 1:2553648/1‑48 (MQ=11)
tACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGgcg > 1:1972418/1‑48 (MQ=11)
aCAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGgcgc > 2:2190977/1‑48 (MQ=11)
aCAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGgcgc > 1:1383250/1‑48 (MQ=11)
aCAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGgcgc > 2:286283/1‑48 (MQ=11)
aCAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGgcgc > 1:2534793/1‑48 (MQ=11)
aCAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGgcgc > 1:509743/1‑48 (MQ=11)
|
CATCATGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGTGTCAGGTGATTCACCGTCACTGACCGGCGTGCCGCCCTGGCGC > NC_017635/2322687‑2322775
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTCATCATGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGTGTCAGGTGATTCACCGTCACTGACCGGCGTGCCGCCCTGGCGCGGGGCG > NC_017635/2322685‑2322781
|
GTCATCATGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTC > M01420:84:000000000‑ABVEH:1:1110:3836:13179/1‑51 (MQ=10)
ATCATGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGG > M01420:84:000000000‑ABVEH:1:1111:20246:5201/1‑51 (MQ=13)
ATCATGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGG < M01420:84:000000000‑ABVEH:1:1109:19209:7380/51‑1 (MQ=13)
GACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATT > M01420:84:000000000‑ABVEH:1:2104:9544:22457/1‑51 (MQ=13)
GACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATT < M01420:84:000000000‑ABVEH:1:2102:20051:18830/51‑1 (MQ=13)
GACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATT > M01420:84:000000000‑ABVEH:1:2113:9832:16084/1‑51 (MQ=13)
ACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTC > M01420:84:000000000‑ABVEH:1:1101:17225:7432/1‑51 (MQ=13)
ACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACC > M01420:84:000000000‑ABVEH:1:1107:7421:24059/1‑51 (MQ=13)
ACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCAC < M01420:84:000000000‑ABVEH:1:1114:10083:11367/50‑1 (MQ=13)
CCTCTGAGAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCC < M01420:84:000000000‑ABVEH:1:2110:8762:16375/51‑1 (MQ=60)
CCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCC < M01420:84:000000000‑ABVEH:1:2110:9001:6612/51‑1 (MQ=13)
CCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCC > M01420:84:000000000‑ABVEH:1:2110:25164:15614/1‑51 (MQ=13)
CCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCC < M01420:84:000000000‑ABVEH:1:1105:17107:4914/51‑1 (MQ=13)
CTGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTC > M01420:84:000000000‑ABVEH:1:2110:12896:17451/1‑50 (MQ=13)
TGAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCAC > M01420:84:000000000‑ABVEH:1:2111:9059:26283/1‑51 (MQ=12)
GAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCACT < M01420:84:000000000‑ABVEH:1:2106:2593:14909/51‑1 (MQ=12)
GAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCAC < M01420:84:000000000‑ABVEH:1:1108:6694:22411/50‑1 (MQ=12)
GAAAAGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCA < M01420:84:000000000‑ABVEH:1:2114:25381:19588/49‑1 (MQ=23)
AGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCACTGACC < M01420:84:000000000‑ABVEH:1:1107:20377:3085/51‑1 (MQ=60)
AGTCGGGCGGTGGACGCCGGTACAGCGTCAGGTGATTCACCCTCACTGACC < M01420:84:000000000‑ABVEH:1:1104:24273:25854/51‑1 (MQ=60)
GGACGCCGGTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCC > M01420:84:000000000‑ABVEH:1:2108:15796:9841/1‑51 (MQ=12)
GGACGCCGGTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCC > M01420:84:000000000‑ABVEH:1:2113:4714:9095/1‑51 (MQ=12)
GGTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGGCGC > M01420:84:000000000‑ABVEH:1:2108:22479:22189/1‑51 (MQ=6)
GGTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGGCGC > M01420:84:000000000‑ABVEH:1:2103:17881:22443/1‑51 (MQ=6)
GGTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGGCGC > M01420:84:000000000‑ABVEH:1:1112:8518:3920/1‑51 (MQ=6)
GTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGGCGCG > M01420:84:000000000‑ABVEH:1:2108:13723:18491/1‑51 (MQ=3)
GTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGGCGCG > M01420:84:000000000‑ABVEH:1:1112:26402:21964/1‑51 (MQ=3)
GTACAGCGTCAGGTGATTCACCCTCACTGACCGGCGTGCCGCCCTGGCGCG > M01420:84:000000000‑ABVEH:1:1105:26464:12798/1‑51 (MQ=3)
GTGTCAGGTGATTCACCGTCACTGACCGGCGTGCCGCCCTGGCGCGGGGCG > M01420:84:000000000‑ABVEH:1:1109:18847:6391/1‑51 (MQ=0)
|
GTCATCATGACTACCTCTGAAAAGTCGGGCGGTGGACGCCGGTACAGTGTCAGGTGATTCACCGTCACTGACCGGCGTGCCGCCCTGGCGCGGGGCG > NC_017635/2322685‑2322781
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
