Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,953,377 T→C T217A (ACG→GCG)  recB ← RecB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,953,3770TC100.0% 620.1 / NA 197T217A (ACG→GCG) recBRecB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (102/95);  total (102/95)

Only 100 of 205 total aligned reads displayed.

TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCC  >  NC_000913/2953287‑2953466
                                                                                          |                                                                                         
 cAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtc                                                                                        >  1:4097223/1‑93 (MQ=255)
 cAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCACGTGACGGGAAGCCAGCGCTtc                                                                                        >  1:4546295/1‑93 (MQ=255)
  aGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtca                                                                                       >  1:589306/1‑93 (MQ=255)
  aGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtca                                                                                       >  1:3284370/1‑93 (MQ=255)
   gcgcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcat                                                                                      >  2:4063419/1‑93 (MQ=255)
    cgcATCCATTTCACCCCCTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcatc                                                                                     <  2:1740257/93‑1 (MQ=255)
    cgcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcatc                                                                                     <  1:1064802/93‑1 (MQ=255)
     gcATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTtcatca                                                                                    >  1:3104623/1‑93 (MQ=255)
      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   >  1:3692746/1‑93 (MQ=255)
      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   <  2:4016581/93‑1 (MQ=255)
      cATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTcatcat                                                                                   <  2:2388354/93‑1 (MQ=255)
       aTCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCatcatc                                                                                  <  2:2724712/93‑1 (MQ=255)
        tCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCg                                                                                 >  1:4220493/1‑93 (MQ=255)
        tCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCg                                                                                 <  2:3607669/93‑1 (MQ=255)
        tCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCg                                                                                 <  1:195615/93‑1 (MQ=255)
         ccAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCgg                                                                                <  2:2588315/93‑1 (MQ=255)
           aGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggc                                                                              <  1:3493388/93‑1 (MQ=255)
            gTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcg                                                                             >  2:3978671/1‑93 (MQ=255)
             ttCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcgg                                                                            >  2:1917324/1‑93 (MQ=255)
             ttCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcgg                                                                            >  2:915371/1‑93 (MQ=255)
              tCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGggcggc                                                                           <  1:1760211/93‑1 (MQ=255)
                aCCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGcggcgg                                                                         <  1:2152024/93‑1 (MQ=255)
                aCCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGcggcgg                                                                         >  1:3578624/1‑93 (MQ=255)
                 cccACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGcggcggg                                                                        >  1:2966398/1‑92 (MQ=255)
                  ccACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTg                                                                       >  2:4606870/1‑93 (MQ=255)
                  ccACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTg                                                                       >  1:441205/1‑93 (MQ=255)
                  ccACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTg                                                                       >  2:4039469/1‑93 (MQ=255)
                  ccACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTg                                                                       >  2:606810/1‑93 (MQ=255)
                   cACTGCGCCGCGCCACTGCTGTTTTACCGTATCAATAAGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTgt                                                                      >  2:2496020/1‑92 (MQ=255)
                     cTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCtt                                                                    >  1:1473414/1‑93 (MQ=255)
                      tGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCttt                                                                   >  1:1201931/1‑93 (MQ=255)
                       gCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTg                                                                  >  1:2209994/1‑93 (MQ=255)
                        cGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGa                                                                 <  2:3110414/93‑1 (MQ=255)
                         gTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGAt                                                                <  1:2721982/93‑1 (MQ=255)
                         gTCGCGCCACTGCTGTTTGACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGAt                                                                <  2:1533173/93‑1 (MQ=255)
                             cgcCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAAcc                                                            <  2:4597182/93‑1 (MQ=255)
                             cgcCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAAcc                                                            <  2:698728/93‑1 (MQ=255)
                               ccACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCgg                                                          >  2:1677308/1‑93 (MQ=255)
                                cACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgc                                                         >  1:3935260/1‑93 (MQ=255)
                                 aCTGCTGTTTTACCGTATCAATACGCGCCGCAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgcg                                                        >  2:4231167/1‑93 (MQ=255)
                                  ctgctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGgcgc                                                       >  2:2610444/1‑93 (MQ=255)
                                    gctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCtt                                                     <  2:1212749/93‑1 (MQ=255)
                                    gctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCtt                                                     >  2:2147103/1‑93 (MQ=255)
                                     ctgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTc                                                    >  2:3663698/1‑93 (MQ=255)
                                      tgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCg                                                   >  1:4459574/1‑93 (MQ=255)
                                      tgTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCg                                                   >  1:3621236/1‑93 (MQ=255)
                                       gTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGc                                                  >  1:1651765/1‑93 (MQ=255)
                                       gTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGc                                                  <  2:4666445/93‑1 (MQ=255)
                                       gTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCCGCGCCTCGc                                                  >  2:2843477/1‑93 (MQ=255)
                                        ttttACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGcc                                                 >  2:1425393/1‑93 (MQ=255)
                                         tttACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCt                                                <  1:1113621/93‑1 (MQ=255)
                                               gTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGa                                          >  1:449293/1‑93 (MQ=255)
                                               gTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGa                                          >  1:4034030/1‑93 (MQ=255)
                                               gTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGa                                          >  1:1315753/1‑93 (MQ=255)
                                               ctATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGa                                          <  2:2808889/92‑1 (MQ=255)
                                                tATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAt                                         >  1:1036776/1‑93 (MQ=255)
                                                 aTCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATa                                        >  2:3217311/1‑93 (MQ=255)
                                                 aTCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATa                                        <  2:916767/93‑1 (MQ=255)
                                                     aTACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGa                                    <  1:4068910/93‑1 (MQ=255)
                                                     aTACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGa                                    <  1:809607/93‑1 (MQ=255)
                                                      tACGCGCCACAATTTGCGCGTGACGGGAAGCCAGTGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAt                                   <  1:265532/93‑1 (MQ=255)
                                                      tACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAt                                   <  2:2954921/93‑1 (MQ=255)
                                                          cgcCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAat                               <  2:3766314/93‑1 (MQ=255)
                                                           gcCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAata                              <  2:1137860/93‑1 (MQ=255)
                                                           gcCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAata                              <  1:1860734/93‑1 (MQ=255)
                                                             cacaATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATc                            <  1:1185470/93‑1 (MQ=255)
                                                              acaATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATcg                           <  2:2066256/93‑1 (MQ=255)
                                                              acaATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATcg                           >  2:1054051/1‑93 (MQ=255)
                                                               caATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATcgc                          <  1:3480599/93‑1 (MQ=255)
                                                               caATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATcgc                          <  2:2269170/93‑1 (MQ=255)
                                                                 aTTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCgcgc                        >  2:495477/1‑93 (MQ=255)
                                                                  tttGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgca                       >  1:127743/1‑93 (MQ=255)
                                                                  tttGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgca                       >  2:483629/1‑93 (MQ=255)
                                                                  tttGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgca                       <  2:351463/93‑1 (MQ=255)
                                                                    tGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgcagc                     >  1:4136633/1‑93 (MQ=255)
                                                                     gcgcGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgcagca                    <  1:4566596/93‑1 (MQ=255)
                                                                      cgcgTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCaa                   <  2:1391049/93‑1 (MQ=255)
                                                                         gTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGc                <  2:3348184/93‑1 (MQ=255)
                                                                          tGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGcc               <  2:2734307/93‑1 (MQ=255)
                                                                            aCGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTg             >  1:227779/1‑93 (MQ=255)
                                                                            aCGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTg             <  1:2389688/93‑1 (MQ=255)
                                                                            aCGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTg             <  2:3953066/93‑1 (MQ=255)
                                                                            aCGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTg             >  1:2488220/1‑93 (MQ=255)
                                                                             cGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGc            <  2:4507259/93‑1 (MQ=255)
                                                                             cGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGc            >  1:3353701/1‑93 (MQ=255)
                                                                              gggAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCg           >  1:3821868/1‑93 (MQ=255)
                                                                               ggAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCgg          >  2:854798/1‑93 (MQ=255)
                                                                               ggAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTAGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCgg          >  1:802065/1‑93 (MQ=255)
                                                                                gAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGc         >  1:1908727/1‑93 (MQ=255)
                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       >  2:2680515/1‑93 (MQ=255)
                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       >  2:1051769/1‑93 (MQ=255)
                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       >  2:3053760/1‑93 (MQ=255)
                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       >  2:817193/1‑93 (MQ=255)
                                                                                  aGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGccc       >  1:85418/1‑93 (MQ=255)
                                                                                   gCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCTGCCCt      >  1:1674571/1‑93 (MQ=255)
                                                                                   gCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCt      <  1:3020789/93‑1 (MQ=255)
                                                                                     cAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCttt    <  1:3842932/93‑1 (MQ=255)
                                                                                     cAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCttt    >  1:2114505/1‑93 (MQ=255)
                                                                                      aGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTc   >  2:3083805/1‑93 (MQ=255)
                                                                                       gcgcTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTcc  <  2:2453557/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCC  >  NC_000913/2953287‑2953466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGG  >  NC_000913/2953284‑2953469
                                                                                             |                                                                                            
CGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGC                                                                                              >  SRR4302535.1878298/1‑94 (MQ=60)
 GATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCT                                                                                             <  SRR4302535.1346990/94‑1 (MQ=60)
 GATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATCTGCGCGTGACGGGAAGCCAGCGCT                                                                                             <  SRR4302535.3494360/94‑1 (MQ=60)
  ATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTT                                                                                            <  SRR4302535.1513590/94‑1 (MQ=60)
   TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTC                                                                                           >  SRR4302535.4125267/1‑94 (MQ=60)
   TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTC                                                                                           <  SRR4302535.3309450/94‑1 (MQ=60)
   TCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCACGTGACGGGAAGCCAGCGCTTC                                                                                           >  SRR4302535.4577381/1‑94 (MQ=60)
    CAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCA                                                                                          >  SRR4302535.593436/1‑94 (MQ=60)
    CAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCA                                                                                          >  SRR4302535.3307201/1‑94 (MQ=60)
     AGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCAT                                                                                         >  SRR4302535.1829062/1‑94 (MQ=60)
     AGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCAT                                                                                         <  SRR4302535.2262092/94‑1 (MQ=60)
      GCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATC                                                                                        <  SRR4302535.4043301/94‑1 (MQ=60)
       CGCATCCAGTTCCCCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCA                                                                                       <  SRR4302535.20092/94‑1 (MQ=60)
       CGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCA                                                                                       >  SRR4302535.3126239/1‑94 (MQ=60)
       CGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCA                                                                                       <  SRR4302535.1072377/94‑1 (MQ=60)
        GCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCAT                                                                                      >  SRR4302535.3718304/1‑94 (MQ=60)
        GCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCAT                                                                                      >  SRR4302535.2992925/1‑94 (MQ=60)
         CATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATC                                                                                     <  SRR4302535.1670623/94‑1 (MQ=60)
          ATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCG                                                                                    >  SRR4302535.4249373/1‑94 (MQ=60)
          ATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCG                                                                                    >  SRR4302535.900739/1‑94 (MQ=60)
           TCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGG                                                                                   <  SRR4302535.196960/94‑1 (MQ=60)
              AGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCG                                                                                <  SRR4302535.3517666/94‑1 (MQ=60)
               GTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGG                                                                               >  SRR4302535.3689965/1‑94 (MQ=60)
                 TCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCG                                                                             <  SRR4302535.1772955/94‑1 (MQ=60)
                  CACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGG                                                                            >  SRR4302535.3603431/1‑94 (MQ=60)
                   CCCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGT                                                                           <  SRR4302535.125524/94‑1 (MQ=60)
                   ACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGT                                                                           <  SRR4302535.2167493/94‑1 (MQ=60)
                   ACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGG                                                                           >  SRR4302535.2987209/1‑94 (MQ=60)
                    CCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTG                                                                          >  SRR4302535.444294/1‑94 (MQ=60)
                       ACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTT                                                                       >  SRR4302535.1483963/1‑94 (MQ=60)
                        CTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTT                                                                      >  SRR4302535.1210559/1‑94 (MQ=60)
                         TGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTG                                                                     <  SRR4302535.2664273/94‑1 (MQ=60)
                         TGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTG                                                                     >  SRR4302535.2225737/1‑94 (MQ=60)
                           CGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGAT                                                                   >  SRR4302535.3581255/1‑94 (MQ=60)
                           CGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGAT                                                                   >  SRR4302535.1102274/1‑94 (MQ=60)
                            GTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATA                                                                  <  SRR4302535.2741426/94‑1 (MQ=60)
                                CGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCG                                                              <  SRR4302535.3118798/94‑1 (MQ=60)
                                CGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCG                                                              <  SRR4302535.1522836/94‑1 (MQ=60)
                                 GCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGG                                                             >  SRR4302535.3758872/1‑94 (MQ=60)
                                  CCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGC                                                            >  SRR4302535.3512184/1‑94 (MQ=60)
                                  CCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGC                                                            <  SRR4302535.2895098/94‑1 (MQ=60)
                                  CCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGC                                                            >  SRR4302535.3962111/1‑94 (MQ=60)
                                   CACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTAATCATCGGGCGGCGGTGCTTTGATAACCGGCG                                                           >  SRR4302535.807222/1‑94 (MQ=60)
                                    ACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGC                                                          >  SRR4302535.455/1‑94 (MQ=60)
                                     CTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGCCGGTGCTTTGATAACCGGCGCT                                                         >  SRR4302535.363223/1‑94 (MQ=60)
                                        CTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCG                                                      >  SRR4302535.3646338/1‑94 (MQ=60)
                                        CTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCG                                                      >  SRR4302535.4490160/1‑94 (MQ=60)
                                        CTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCG                                                      >  SRR4302535.1878294/1‑94 (MQ=60)
                                         TGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGATGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGC                                                     <  SRR4302535.1532034/94‑1 (MQ=60)
                                         TGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGC                                                     >  SRR4302535.1663710/1‑94 (MQ=60)
                                         TGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGAGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGC                                                     >  SRR4302535.3254811/1‑94 (MQ=60)
                                          GTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCC                                                    >  SRR4302535.4094402/1‑94 (MQ=60)
                                          GTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCC                                                    >  SRR4302535.1945223/1‑94 (MQ=60)
                                            TTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTT                                                  <  SRR4302535.1121562/94‑1 (MQ=60)
                                            TGTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTT                                                  <  SRR4302535.4596949/94‑1 (MQ=60)
                                                 CGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGA                                             >  SRR4302535.4061647/1‑94 (MQ=60)
                                                 CGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGA                                             >  SRR4302535.452440/1‑94 (MQ=60)
                                                 CGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGA                                             >  SRR4302535.1325151/1‑94 (MQ=60)
                                                  GTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAT                                            <  SRR4302535.814655/94‑1 (MQ=60)
                                                  GTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAT                                            >  SRR4302535.1044140/1‑94 (MQ=60)
                                                  CTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAT                                            <  SRR4302535.4392935/94‑1 (MQ=60)
                                                   TATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATA                                           <  SRR4302535.4224914/94‑1 (MQ=60)
                                                   TATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATA                                           <  SRR4302535.4109193/94‑1 (MQ=60)
                                                      CAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACG                                        >  SRR4302535.4512707/1‑94 (MQ=60)
                                                      CAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACG                                        <  SRR4302535.106580/94‑1 (MQ=60)
                                                       AATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGA                                       <  SRR4302535.1210109/94‑1 (MQ=60)
                                                        ATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAT                                      <  SRR4302535.815270/94‑1 (MQ=60)
                                                        ATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAT                                      <  SRR4302535.532569/94‑1 (MQ=60)
                                                        ATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGAT                                      <  SRR4302535.4096757/94‑1 (MQ=60)
                                                         TACGCGCCACAATTTGCGCGTGACGGGAAGCCAGTGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATT                                     <  SRR4302535.267376/94‑1 (MQ=60)
                                                             CGCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATA                                 <  SRR4302535.2160911/94‑1 (MQ=60)
                                                              GCCACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATAT                                <  SRR4302535.1874150/94‑1 (MQ=60)
                                                                CACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCG                              >  SRR4302535.3445509/1‑94 (MQ=60)
                                                                CACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCG                              <  SRR4302535.1193939/94‑1 (MQ=60)
                                                                 ACAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGC                             >  SRR4302535.1175801/1‑94 (MQ=60)
                                                                  CAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCG                            >  SRR4302535.3935786/1‑94 (MQ=60)
                                                                  CAATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCG                            <  SRR4302535.3504778/94‑1 (MQ=60)
                                                                   AATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGC                           >  SRR4302535.2576793/1‑94 (MQ=60)
                                                                    ATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCA                          >  SRR4302535.4290243/1‑94 (MQ=60)
                                                                    ATTTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCA                          >  SRR4302535.128646/1‑94 (MQ=60)
                                                                      TTGCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGC                        >  SRR4302535.4164956/1‑94 (MQ=60)
                                                                        GCGCGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAA                      <  SRR4302535.4597747/94‑1 (MQ=60)
                                                                           CGTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGC                   <  SRR4302535.838825/94‑1 (MQ=60)
                                                                            GTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCC                  <  SRR4302535.2707053/94‑1 (MQ=60)
                                                                            GTGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCC                  <  SRR4302535.3861849/94‑1 (MQ=60)
                                                                             TGACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCT                 >  SRR4302535.4485357/1‑94 (MQ=60)
                                                                              GACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTG                >  SRR4302535.2506163/1‑94 (MQ=60)
                                                                              GACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTG                >  SRR4302535.229345/1‑94 (MQ=60)
                                                                               ACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGC               >  SRR4302535.3377055/1‑94 (MQ=60)
                                                                               ACGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGC               <  SRR4302535.2406867/94‑1 (MQ=60)
                                                                                CGGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCG              >  SRR4302535.3848234/1‑94 (MQ=60)
                                                                                 GGGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTAGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGG             >  SRR4302535.807700/1‑94 (MQ=60)
                                                                                  GGAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGC            >  SRR4302535.1922487/1‑94 (MQ=60)
                                                                                   GAAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCC           >  SRR4302535.1732664/1‑94 (MQ=60)
                                                                                    AAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCC          >  SRR4302535.86018/1‑94 (MQ=60)
                                                                                    AAGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCC          <  SRR4302535.1472118/94‑1 (MQ=60)
                                                                                     AGCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCTGCCCT         >  SRR4302535.1686695/1‑94 (MQ=60)
                                                                                      GCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTT        >  SRR4302535.2487166/1‑94 (MQ=60)
                                                                                      GCCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTT        <  SRR4302535.3041944/94‑1 (MQ=60)
                                                                                       CCAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTT       >  SRR4302535.2129766/1‑94 (MQ=60)
                                                                                        CAGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTC      <  SRR4302535.3869403/94‑1 (MQ=60)
                                                                                         AGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCC     <  SRR4302535.23076/94‑1 (MQ=60)
                                                                                         AGCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCC     >  SRR4302535.2188755/1‑94 (MQ=60)
                                                                                          GCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCA    <  SRR4302535.1288005/94‑1 (MQ=60)
                                                                                          GCGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCA    <  SRR4302535.4135401/94‑1 (MQ=60)
                                                                                           CGCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAG   >  SRR4302535.4077889/1‑94 (MQ=60)
                                                                                            GCTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGAGAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGG  <  SRR4302535.98706/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGG  >  NC_000913/2953284‑2953469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑