Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,979,391 T→C T272A (ACC→GCC)  araE ← low‑affinity L‑arabinose transport system proton symport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,979,3910TC99.5% 585.0 / NA 187T272A (ACC→GCC) araElow‑affinity L‑arabinose transport system proton symport protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (97/89);  minor base A (0/1);  total (97/90)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

CCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTT  >  NC_000913/2979301‑2979480
                                                                                          |                                                                                         
 cAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGTTACGCGGCGCGTAGTACATAATGATGTTCATACCGGCaaa                                                                                        >  2:3880310/1‑93 (MQ=255)
 cAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCaaa                                                                                        >  1:1145822/1‑93 (MQ=255)
  agagTCGCAATCATCTGTTGTTCTGTGGTCTTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAc                                                                                       <  2:3999643/93‑1 (MQ=255)
  agagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAc                                                                                       <  1:1105955/93‑1 (MQ=255)
  agagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAc                                                                                       <  2:2170315/93‑1 (MQ=255)
  agagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAc                                                                                       <  1:3508065/93‑1 (MQ=255)
  agagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAc                                                                                       >  2:2604668/1‑93 (MQ=255)
  agagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAc                                                                                       <  1:434744/93‑1 (MQ=255)
   gagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAct                                                                                      >  1:51114/1‑93 (MQ=255)
   gagTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAct                                                                                      >  1:3436382/1‑93 (MQ=255)
     gTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAActgc                                                                                    <  1:763401/93‑1 (MQ=255)
     gTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAActgc                                                                                    <  2:306918/93‑1 (MQ=255)
        gCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACtgctgc                                                                                 >  2:4125934/1‑93 (MQ=255)
        gCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACtgctgc                                                                                 >  1:3230803/1‑93 (MQ=255)
         cAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCa                                                                                >  1:2772803/1‑93 (MQ=255)
         cAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCa                                                                                <  1:194648/93‑1 (MQ=255)
         cAATCAGCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCa                                                                                <  1:4108062/93‑1 (MQ=255)
          aaTCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCAt                                                                               <  1:473528/93‑1 (MQ=255)
          aaTCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCAt                                                                               >  2:766995/1‑93 (MQ=255)
           atcatcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATc                                                                              <  1:85350/93‑1 (MQ=255)
           atcatcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATc                                                                              <  2:4239999/93‑1 (MQ=255)
           atcatcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATc                                                                              <  1:990763/93‑1 (MQ=255)
             catcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGc                                                                            >  2:4634303/1‑93 (MQ=255)
              atcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGcc                                                                           <  1:4558559/93‑1 (MQ=255)
              atcTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGcc                                                                           <  1:4550033/93‑1 (MQ=255)
              atcTGTTGTTCTGTAGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCTAACTGCTTCATCGcc                                                                           >  2:2052108/1‑93 (MQ=255)
                 tgttgtTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGc                                                                        <  1:4185096/93‑1 (MQ=255)
                  gttgttCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGca                                                                       >  1:3725849/1‑93 (MQ=255)
                   ttgttCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGcaa                                                                      >  1:4272689/1‑93 (MQ=255)
                      ttCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGcaacaa                                                                   >  1:3058041/1‑93 (MQ=255)
                      ttCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGcaacaa                                                                   <  2:3847811/93‑1 (MQ=255)
                       tCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCaacaac                                                                  <  2:1373490/93‑1 (MQ=255)
                        cTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGCTGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAacaaca                                                                 <  1:2514791/93‑1 (MQ=255)
                        cTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAacaaca                                                                 <  1:4082498/93‑1 (MQ=255)
                         tgtgGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACAt                                                                >  2:895327/1‑93 (MQ=255)
                          gtgGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATa                                                               >  1:1531193/1‑93 (MQ=255)
                          gtgGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATa                                                               <  1:1698857/93‑1 (MQ=255)
                            ggTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATAcc                                                             <  1:3152396/93‑1 (MQ=255)
                            ggTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATAcc                                                             >  2:4517796/1‑93 (MQ=255)
                                gTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCgaga                                                         <  2:2068022/93‑1 (MQ=255)
                                  aaaGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGaaa                                                       >  2:3259563/1‑93 (MQ=255)
                                   aaGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAac                                                      >  2:2856001/1‑93 (MQ=255)
                                   aaGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAac                                                      >  2:3683274/1‑93 (MQ=255)
                                     gCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAacac                                                    >  2:2360997/1‑93 (MQ=255)
                                     gCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAacac                                                    >  1:699827/1‑93 (MQ=255)
                                     gCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAacac                                                    <  1:2461815/93‑1 (MQ=255)
                                     gCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAacac                                                    <  1:3806346/93‑1 (MQ=255)
                                      cccGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAAcaca                                                   <  1:1515550/93‑1 (MQ=255)
                                       ccGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAg                                                  <  2:4254153/93‑1 (MQ=255)
                                        cGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGc                                                 <  1:2806796/93‑1 (MQ=255)
                                         gCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCa                                                >  1:4312128/1‑93 (MQ=255)
                                            aTTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacga                                             >  1:642667/1‑93 (MQ=255)
                                            aTTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacga                                             >  2:1860914/1‑93 (MQ=255)
                                             ttttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacgac                                            >  1:96718/1‑93 (MQ=255)
                                             ttttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacgac                                            >  2:1104298/1‑93 (MQ=255)
                                             ttttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacgac                                            >  2:3159539/1‑93 (MQ=255)
                                             ttttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacgac                                            >  1:4092055/1‑93 (MQ=255)
                                             ttttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacgac                                            <  1:3661945/93‑1 (MQ=255)
                                             ttttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCacaac                                            >  2:1660730/1‑93 (MQ=255)
                                               ttGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacgg                                          <  1:3268988/93‑1 (MQ=255)
                                                tGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacgga                                         <  2:1247773/93‑1 (MQ=255)
                                                tGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacgga                                         <  2:1334592/93‑1 (MQ=255)
                                                tGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGAAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacgga                                         <  1:2276785/93‑1 (MQ=255)
                                                 gAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacggac                                        >  2:2845331/1‑93 (MQ=255)
                                                 gAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacggac                                        >  2:4471208/1‑93 (MQ=255)
                                                 gAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacggac                                        <  2:637030/93‑1 (MQ=255)
                                                  aaGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACgacggacg                                       >  2:2802654/1‑93 (MQ=255)
                                                    gATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGtt                                     >  1:1635939/1‑93 (MQ=255)
                                                        cgcgGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACgg                                 <  1:367202/93‑1 (MQ=255)
                                                        cgcgGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACgg                                 <  1:2135781/93‑1 (MQ=255)
                                                         gcggcgCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGt                                <  2:2410421/93‑1 (MQ=255)
                                                         gcggcgCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGt                                <  1:866246/93‑1 (MQ=255)
                                                           ggcgCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTg                              <  1:1319844/93‑1 (MQ=255)
                                                            gcgcGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGa                             <  2:1782942/93‑1 (MQ=255)
                                                            gcgcGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGa                             >  2:1174850/1‑93 (MQ=255)
                                                               cgTAGTACATGATGATGTTCATACCGGCAACCTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGAACGACGGACGTTACGGTTGATCt                          <  2:2268662/93‑1 (MQ=255)
                                                               cgTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCt                          >  2:904695/1‑93 (MQ=255)
                                                                 tagtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTa                        >  2:2045566/1‑93 (MQ=255)
                                                                 tagtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTa                        >  2:337877/1‑93 (MQ=255)
                                                                 gagtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATATTa                        >  2:4420984/2‑93 (MQ=255)
                                                                  agtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTaa                       >  2:1979031/1‑93 (MQ=255)
                                                                  agtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTAATCTTaa                       <  2:3748253/93‑1 (MQ=255)
                                                                   gtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTaaa                      <  2:3210597/93‑1 (MQ=255)
                                                                   gtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTaaa                      >  1:1185359/1‑93 (MQ=255)
                                                                   gtaCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTaaa                      <  1:3953409/93‑1 (MQ=255)
                                                                    taCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAAc                     <  1:2230788/93‑1 (MQ=255)
                                                                     aCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACa                    <  1:920470/93‑1 (MQ=255)
                                                                     aCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACa                    >  1:4614176/1‑93 (MQ=255)
                                                                       atgatgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGt                  >  1:517276/1‑93 (MQ=255)
                                                                        tgatgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTg                 >  2:1031544/1‑93 (MQ=255)
                                                                         gatgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGc                <  1:4631085/93‑1 (MQ=255)
                                                                         gatgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGc                >  2:1196075/1‑93 (MQ=255)
                                                                          atgatgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGcc               >  1:3734498/1‑93 (MQ=255)
                                                                            gctgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGCCGTTACGGTTGATCTTACACAGTGCCCa             >  2:505155/3‑93 (MQ=255)
                                                                             atgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCaa            >  2:1256547/1‑93 (MQ=255)
                                                                              tgTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAAc           <  1:1026820/93‑1 (MQ=255)
                                                                               gTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAAcc          >  1:2320669/1‑93 (MQ=255)
                                                                                   aTACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGccc      <  1:1738485/93‑1 (MQ=255)
                                                                                    tACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCt     <  2:942308/93‑1 (MQ=255)
                                                                                     aCCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTg    >  1:2878745/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTT  >  NC_000913/2979301‑2979480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTT  >  NC_000913/2979300‑2979482
                                                                                           |                                                                                           
ACCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAA                                                                                           >  SRR4302535.4013885/1‑94 (MQ=60)
 CCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAA                                                                                          >  SRR4302535.1153988/1‑94 (MQ=60)
  CAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAAC                                                                                         <  SRR4302535.1187402/94‑1 (MQ=60)
   AGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACT                                                                                        <  SRR4302535.1113856/94‑1 (MQ=60)
   AGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACT                                                                                        >  SRR4302535.51492/1‑94 (MQ=60)
   AGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACT                                                                                        <  SRR4302535.437773/94‑1 (MQ=60)
   AGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACT                                                                                        >  SRR4302535.3460281/1‑94 (MQ=60)
   AGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACT                                                                                        <  SRR4302535.3532445/94‑1 (MQ=60)
    GAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTG                                                                                       >  SRR4302535.681982/1‑94 (MQ=60)
     AGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGC                                                                                      >  SRR4302535.1999028/1‑94 (MQ=60)
      GTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCT                                                                                     <  SRR4302535.768779/94‑1 (MQ=60)
        CGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGC                                                                                   >  SRR4302535.3253203/1‑94 (MQ=60)
         GCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCA                                                                                  >  SRR4302535.2792546/1‑94 (MQ=60)
          CAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCAT                                                                                 <  SRR4302535.195988/94‑1 (MQ=60)
          CAATCAGCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCAT                                                                                 <  SRR4302535.4136189/94‑1 (MQ=60)
           AATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATC                                                                                >  SRR4302535.4352095/1‑94 (MQ=60)
           AATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATC                                                                                <  SRR4302535.476843/94‑1 (MQ=60)
            ATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCG                                                                               <  SRR4302535.4171263/94‑1 (MQ=60)
            ATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCG                                                                               <  SRR4302535.85950/94‑1 (MQ=60)
            ATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCG                                                                               <  SRR4302535.997796/94‑1 (MQ=60)
              CATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCC                                                                             <  SRR4302535.3866862/94‑1 (MQ=60)
               ATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCT                                                                            >  SRR4302535.2828799/1‑94 (MQ=60)
               ATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCT                                                                            <  SRR4302535.2795632/94‑1 (MQ=60)
               ATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCT                                                                            <  SRR4302535.4639164/94‑1 (MQ=60)
               ATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCT                                                                            >  SRR4302535.821705/1‑94 (MQ=60)
               ATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCT                                                                            <  SRR4302535.4589683/94‑1 (MQ=60)
               ATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCT                                                                            <  SRR4302535.4581136/94‑1 (MQ=60)
                TCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTG                                                                           <  SRR4302535.3188463/94‑1 (MQ=60)
                 CTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGC                                                                          >  SRR4302535.1361451/1‑94 (MQ=60)
                  TGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCA                                                                         >  SRR4302535.3751663/1‑94 (MQ=60)
                  TGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCA                                                                         <  SRR4302535.4213726/94‑1 (MQ=60)
                  TGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCA                                                                         >  SRR4302535.623623/1‑94 (MQ=60)
                   GTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAA                                                                        >  SRR4302535.4301937/1‑94 (MQ=60)
                    TTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAAC                                                                       >  SRR4302535.2288291/1‑94 (MQ=60)
                    TTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAAC                                                                       >  SRR4302535.3563759/1‑94 (MQ=60)
                      GTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAA                                                                     >  SRR4302535.3079433/1‑94 (MQ=60)
                       TTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAAC                                                                    <  SRR4302535.4278300/94‑1 (MQ=60)
                         CTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGCTGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACAT                                                                  <  SRR4302535.2532920/94‑1 (MQ=60)
                         CTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACAT                                                                  <  SRR4302535.4110421/94‑1 (MQ=60)
                          TGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATA                                                                 >  SRR4302535.1542223/1‑94 (MQ=60)
                           GTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATAC                                                                <  SRR4302535.1711135/94‑1 (MQ=60)
                            TGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACC                                                               <  SRR4302535.124358/94‑1 (MQ=60)
                             GGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCG                                                              >  SRR4302535.3427461/1‑94 (MQ=60)
                             GGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCG                                                              <  SRR4302535.3174302/94‑1 (MQ=60)
                                  TAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAA                                                         >  SRR4302535.1119442/1‑94 (MQ=60)
                                    AAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACA                                                       >  SRR4302535.993112/1‑94 (MQ=60)
                                    AAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACA                                                       <  SRR4302535.2974185/94‑1 (MQ=60)
                                     AGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACAC                                                      >  SRR4302535.704737/1‑94 (MQ=60)
                                      GCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACA                                                     <  SRR4302535.3832592/94‑1 (MQ=60)
                                      GCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACA                                                     <  SRR4302535.2479562/94‑1 (MQ=60)
                                       CCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAG                                                    >  SRR4302535.4335772/1‑94 (MQ=60)
                                       CCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAG                                                    <  SRR4302535.1526468/94‑1 (MQ=60)
                                        CCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGC                                                   <  SRR4302535.4571491/94‑1 (MQ=60)
                                         CGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCA                                                  <  SRR4302535.1774076/94‑1 (MQ=60)
                                         CGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCA                                                  >  SRR4302535.4341628/1‑94 (MQ=60)
                                         CGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCA                                                  <  SRR4302535.2826749/94‑1 (MQ=60)
                                          GCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCAC                                                 >  SRR4302535.736520/1‑94 (MQ=60)
                                           CCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACG                                                <  SRR4302535.2570832/94‑1 (MQ=60)
                                           CCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACG                                                >  SRR4302535.1533605/1‑94 (MQ=60)
                                            CATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGA                                               <  SRR4302535.3758022/94‑1 (MQ=60)
                                            CATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGA                                               >  SRR4302535.958560/1‑94 (MQ=60)
                                            CATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGA                                               >  SRR4302535.647155/1‑94 (MQ=60)
                                             ATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGAC                                              >  SRR4302535.97401/1‑94 (MQ=60)
                                             ATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGAC                                              >  SRR4302535.4120056/1‑94 (MQ=60)
                                              TTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACG                                             <  SRR4302535.3687299/94‑1 (MQ=60)
                                                TTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGA                                           <  SRR4302535.3291697/94‑1 (MQ=60)
                                                 TGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGAC                                          <  SRR4302535.2305261/94‑1 (MQ=60)
                                                 TGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGAAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGAC                                          <  SRR4302535.2293042/94‑1 (MQ=60)
                                                    AGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTT                                       >  SRR4302535.1647760/1‑94 (MQ=60)
                                                    AGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTT                                       <  SRR4302535.4666619/94‑1 (MQ=60)
                                                         CGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGT                                  <  SRR4302535.2151169/94‑1 (MQ=60)
                                                         CGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGT                                  <  SRR4302535.2513752/94‑1 (MQ=60)
                                                         CGCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGT                                  <  SRR4302535.369734/94‑1 (MQ=60)
                                                          GCGGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTT                                 <  SRR4302535.872372/94‑1 (MQ=60)
                                                            GGCGCGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGA                               <  SRR4302535.1329275/94‑1 (MQ=60)
                                                                CGTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTT                           <  SRR4302535.906210/94‑1 (MQ=60)
                                                                 GTAGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTA                          <  SRR4302535.1638049/94‑1 (MQ=60)
                                                                   AGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAA                        >  SRR4302535.1193828/1‑94 (MQ=60)
                                                                   AGTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAA                        >  SRR4302535.2394182/1‑94 (MQ=60)
                                                                    GTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAAC                       <  SRR4302535.458369/94‑1 (MQ=60)
                                                                    GTACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAAC                       <  SRR4302535.3980398/94‑1 (MQ=60)
                                                                     TACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACA                      >  SRR4302535.4645526/1‑94 (MQ=60)
                                                                     TACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACA                      <  SRR4302535.2246650/94‑1 (MQ=60)
                                                                     TACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACAAAGCACGACGGACGTTACGGTTGATCTTAAACA                      >  SRR4302535.868262/1‑94 (MQ=60)
                                                                      TCATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAG                     <  SRR4302535.3787965/94‑1 (MQ=60)
                                                                      ACATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAG                     <  SRR4302535.927001/94‑1 (MQ=60)
                                                                       CATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGT                    >  SRR4302535.520894/1‑94 (MQ=60)
                                                                        ATGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTG                   <  SRR4302535.1704435/94‑1 (MQ=60)
                                                                         TGATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGC                  >  SRR4302535.3499890/1‑94 (MQ=60)
                                                                          GATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCC                 >  SRR4302535.4660396/1‑94 (MQ=60)
                                                                          GATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCC                 <  SRR4302535.4662508/94‑1 (MQ=60)
                                                                          GATGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCC                 >  SRR4302535.3760345/1‑94 (MQ=60)
                                                                            TGATGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCA               >  SRR4302535.4114623/1‑94 (MQ=60)
                                                                               TGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACC            >  SRR4302535.2337279/1‑94 (MQ=60)
                                                                               TGTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACC            <  SRR4302535.1034111/94‑1 (MQ=60)
                                                                                GTTCATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCG           <  SRR4302535.491694/94‑1 (MQ=60)
                                                                                    ATACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCT       <  SRR4302535.1751051/94‑1 (MQ=60)
                                                                                     TACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTG      >  SRR4302535.2899129/1‑94 (MQ=60)
                                                                                     TACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTG      <  SRR4302535.1728730/94‑1 (MQ=60)
                                                                                     TACCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTG      >  SRR4302535.3720603/1‑94 (MQ=60)
                                                                                       CCGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTT    <  SRR4302535.3691707/94‑1 (MQ=60)
                                                                                        CGGCAAACTGCTGCATCGCCTGCAACAACATGCCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTT   <  SRR4302535.310117/94‑1 (MQ=60)
                                                                                        CGGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTT   >  SRR4302535.345957/1‑94 (MQ=60)
                                                                                         GGCAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTT  >  SRR4302535.2599629/1‑94 (MQ=60)
                                                                                           |                                                                                           
ACCAGAGTCGCAATCATCTGTTGTTCTGTGGTCGTAAAGCCCGCCATTTTGAAGATACGCGGCGCGTAGTACATGATGATGTTCATACCGGTAAACTGCTGCATCGCCTGCAACAACATACCGAGAAACACAGCACGACGGACGTTACGGTTGATCTTAAACAGTGCCCAACCGCCCTGTTTT  >  NC_000913/2979300‑2979482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑