Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,893	+C	coding (405/423 nt)	yhaQ ←	putative L‑serine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,891	1	.	C	99.1%	679.4 / ‑1.5	214	coding (407/423 nt)	yhaQ	putative L‑serine dehydratase
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (110/102); total (111/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 240 total aligned reads displayed.

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA  >  NC_000913/3256800‑3256978

tttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑c                                                                                         >  1:2014242/1‑93 (MQ=255)

tttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑c                                                                                         >  1:651769/1‑93 (MQ=255)

tttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑c                                                                                         >  1:1834321/1‑93 (MQ=255)

 ttCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cc                                                                                        >  2:4185960/1‑93 (MQ=255)

  tCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccc                                                                                       <  2:1937738/93‑2 (MQ=255)

  tCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccc                                                                                       <  1:852942/93‑2 (MQ=255)

  tCGACAGCGTGCGACAGACCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccc                                                                                       <  2:1693324/93‑2 (MQ=255)

   cGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccct                                                                                      >  2:2338029/1‑91 (MQ=255)

    gACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccctt                                                                                     >  2:478759/1‑90 (MQ=255)

    gACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑ccctt                                                                                     >  1:2717077/1‑90 (MQ=255)

     aCATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑cccttc                                                                                    <  1:288723/93‑5 (MQ=255)

       aTCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCa                                                                                  <  2:3986324/93‑1 (MQ=255)

        tCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAg                                                                                 >  2:4378330/1‑93 (MQ=255)

         cGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAgg                                                                                <  2:2682857/93‑1 (MQ=255)

          gTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTTCCAGGc                                                                               <  2:4578204/93‑1 (MQ=255)

            gCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAg                                                                             >  2:118142/1‑93 (MQ=255)

            gCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAg                                                                             <  1:57214/93‑1 (MQ=255)

            gCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAg                                                                             <  2:295549/93‑1 (MQ=255)

             cGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGt                                                                            <  1:4561612/93‑1 (MQ=255)

             cGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGt                                                                            <  2:403909/93‑1 (MQ=255)

              gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTg                                                                           >  2:3180987/1‑93 (MQ=255)

              gACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTg                                                                           >  1:3086216/1‑93 (MQ=255)

               aCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGa                                                                          >  2:310396/1‑93 (MQ=255)

               aCAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGa                                                                          >  1:1203924/1‑93 (MQ=255)

                 aGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATc                                                                        <  2:2575415/93‑1 (MQ=255)

                  ggCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATcc                                                                       >  2:3191929/1‑93 (MQ=255)

                  ggCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATcc                                                                       >  2:3363092/1‑93 (MQ=255)

                   gCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCg                                                                      >  2:1839672/1‑93 (MQ=255)

                     cGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATGCGCa                                                                    >  2:4425047/1‑93 (MQ=255)

                      gAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCAt                                                                   >  1:1003601/1‑93 (MQ=255)

                      gAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCAt                                                                   >  2:4081958/1‑93 (MQ=255)

                       aaGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCAtt                                                                  >  2:2102670/1‑93 (MQ=255)

                        aGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTc                                                                 >  2:3557194/1‑93 (MQ=255)

                         gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTcc                                                                >  2:3409953/1‑93 (MQ=255)

                         gtgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTcc                                                                >  1:4290384/1‑93 (MQ=255)

                         gtgtTCTTCTTCAACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTcc                                                                <  1:1650976/93‑1 (MQ=255)

                          tgtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCg                                                               >  2:461840/1‑93 (MQ=255)

                           gtTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGt                                                              >  1:3345331/1‑93 (MQ=255)

                            ttcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGtt                                                             >  1:4135029/1‑93 (MQ=255)

                            ttcttcttcGACAATAAACCCGCCGCCGACAGAGGAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGtt                                                             >  2:1503578/1‑93 (MQ=255)

                             tcttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTtc                                                            >  1:4619710/1‑93 (MQ=255)

                              cttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTtct                                                           >  2:2171337/1‑93 (MQ=255)

                               ttcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTtctc                                                          >  2:4319654/1‑93 (MQ=255)

                                tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCa                                                         <  1:257205/93‑1 (MQ=255)

                                tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCa                                                         >  1:4348972/1‑93 (MQ=255)

                                tcttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCa                                                         >  2:1594052/1‑93 (MQ=255)

                                  ttcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATg                                                       >  1:1543359/1‑93 (MQ=255)

                                   tcGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATgg                                                      >  2:79085/1‑93 (MQ=255)

                                    cGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGc                                                     <  2:2282064/93‑1 (MQ=255)

                                      aCAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGa                                                   <  2:348429/93‑1 (MQ=255)

                                       cAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg                                                  >  2:3727400/1‑93 (MQ=255)

                                       cAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAg                                                  >  1:2211512/1‑93 (MQ=255)

                                         aTAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGc                                                >  1:1273368/1‑93 (MQ=255)

                                          tAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCa                                               >  1:311533/1‑93 (MQ=255)

                                           aaccccGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa                                              <  1:771798/90‑1 (MQ=255)

                                           aaaCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCaa                                              <  2:2631265/93‑1 (MQ=255)

                                             aCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACa                                            <  1:936699/93‑1 (MQ=255)

                                               ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAtt                                          >  1:4010690/1‑93 (MQ=255)

                                               ccgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAtt                                          <  1:3968038/93‑1 (MQ=255)

                                                cgccgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAttt                                         <  1:3792604/93‑1 (MQ=255)

                                                   cgccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCgg                                      >  2:697020/1‑93 (MQ=255)

                                                    gccgACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCggg                                     <  1:1557012/93‑1 (MQ=255)

                                                       gACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATg                                  >  1:1041889/1‑93 (MQ=255)

                                                        aCAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATgg                                 >  2:2470521/1‑93 (MQ=255)

                                                         cAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGa                                <  1:897736/93‑1 (MQ=255)

                                                         cAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGa                                <  1:4499930/93‑1 (MQ=255)

                                                         cAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGa                                <  2:2189941/93‑1 (MQ=255)

                                                          agagTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGaa                               <  2:887382/93‑1 (MQ=255)

                                                             gTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAt                            >  1:1018868/1‑93 (MQ=255)

                                                             gTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAt                            <  2:2981007/93‑1 (MQ=255)

                                                             gTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAt                            <  1:274747/93‑1 (MQ=255)

                                                              tAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATa                           >  1:453814/1‑93 (MQ=255)

                                                               aaTAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATaa                          <  2:1142647/93‑1 (MQ=255)

                                                                aTAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAt                         >  1:2872354/1‑93 (MQ=255)

                                                                 tAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg                        <  2:634269/93‑1 (MQ=255)

                                                                 tAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATg                        <  1:2918175/93‑1 (MQ=255)

                                                                  aGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt                       >  1:3520693/1‑93 (MQ=255)

                                                                  aGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt                       <  1:3711356/93‑1 (MQ=255)

                                                                  aGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt                       <  1:722975/93‑1 (MQ=255)

                                                                  aGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGt                       <  1:1893184/93‑1 (MQ=255)

                                                                    gTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTc                     >  2:529498/1‑93 (MQ=255)

                                                                    gTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTc                     <  1:2000006/93‑1 (MQ=255)

                                                                     ttttACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCt                    <  1:1141908/93‑1 (MQ=255)

                                                                     ttttACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCt                    >  1:169441/1‑93 (MQ=255)

                                                                       ttACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGGTAATGTTCttt                  >  2:3523754/1‑93 (MQ=255)

                                                                       ttACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCttt                  <  2:2805539/93‑1 (MQ=255)

                                                                       ttACTTAATAGCTCTGCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCttt                  <  2:415969/93‑1 (MQ=255)

                                                                         aCTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGc                >  1:3284538/1‑93 (MQ=255)

                                                                            tAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAc             >  1:1319478/1‑93 (MQ=255)

                                                                             aaTAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACa            <  1:2653935/93‑1 (MQ=255)

                                                                              aTAGCTCTTCCTGTCCC‑TTCCAGGGAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAg           <  1:4488748/93‑1 (MQ=255)

                                                                              aTAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAg           <  1:2479904/93‑1 (MQ=255)

                                                                                aGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGa         >  1:1778987/1‑93 (MQ=255)

                                                                                aGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGa         >  2:58239/1‑93 (MQ=255)

                                                                                  ctctTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaa       >  1:135969/1‑93 (MQ=255)

                                                                                   tctTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaaa      <  1:1232872/93‑1 (MQ=255)

                                                                                   tctTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGaaaa      <  1:777482/93‑1 (MQ=255)

                                                                                    ctTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAt     >  1:1074460/1‑93 (MQ=255)

                                                                                     ttCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCAGGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATc    >  2:3826560/1‑93 (MQ=255)

                                                                                       ccTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATcaa  <  2:3674030/93‑1 (MQ=255)

TTTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAA  >  NC_000913/3256800‑3256978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  NC_000913/3256801‑3256986

TTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CC‑C                                                                                              <  SRR4302535.2315394/94‑1 (MQ=60)

 TCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CC‑CT                                                                                             <  SRR4302535.858948/94‑1 (MQ=60)

  CGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCctt                                                                                             >  SRR4302535.2736486/1‑91 (MQ=60)

    ACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCC                                                                                           <  SRR4302535.290745/94‑1 (MQ=60)

    ACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCC                                                                                           >  SRR4302535.1702953/1‑94 (MQ=60)

     CATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCA                                                                                          <  SRR4302535.3053676/94‑1 (MQ=60)

     CATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCA                                                                                          <  SRR4302535.4207546/94‑1 (MQ=60)

        CGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGC                                                                                       <  SRR4302535.4505487/94‑1 (MQ=60)

           TCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGT                                                                                    <  SRR4302535.337780/94‑1 (MQ=60)

           GCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGT                                                                                    <  SRR4302535.57630/94‑1 (MQ=60)

            CGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTG                                                                                   >  SRR4302535.3107710/1‑94 (MQ=60)

            CGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTG                                                                                   <  SRR4302535.4592744/94‑1 (MQ=60)

             GACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGA                                                                                  >  SRR4302535.2896984/1‑94 (MQ=60)

             GACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGA                                                                                  >  SRR4302535.1212565/1‑94 (MQ=60)

               CAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATC                                                                                >  SRR4302535.2018591/1‑94 (MQ=60)

                 GGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCG                                                                              >  SRR4302535.3917694/1‑94 (MQ=60)

                   CCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCA                                                                            >  SRR4302535.1802053/1‑94 (MQ=60)

                    CGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCAT                                                                           >  SRR4302535.1010725/1‑94 (MQ=60)

                       AGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCC                                                                        >  SRR4302535.4319729/1‑94 (MQ=60)

                       AGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCC                                                                        >  SRR4302535.2869380/1‑94 (MQ=60)

                        GTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCG                                                                       <  SRR4302535.1316742/94‑1 (MQ=60)

                        GTGTTCTTCTTCAACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCG                                                                       <  SRR4302535.1662913/94‑1 (MQ=60)

                         TGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGT                                                                      >  SRR4302535.3368633/1‑94 (MQ=60)

                          GTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTT                                                                     >  SRR4302535.4163344/1‑94 (MQ=60)

                           TTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTC                                                                    >  SRR4302535.1351647/1‑94 (MQ=60)

                           TTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTC                                                                    >  SRR4302535.4651100/1‑94 (MQ=60)

                             CTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTC                                                                  >  SRR4302535.3860713/1‑94 (MQ=60)

                             CTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CCTTTACAGGCAGTGATTCGCATTCCGTTCTC                                                                  >  SRR4302535.1706463/1‑94 (MQ=60)

                              TTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCA                                                                 >  SRR4302535.2943191/1‑94 (MQ=60)

                              TTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCA                                                                 >  SRR4302535.4378724/1‑94 (MQ=60)

                               TCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCAT                                                                <  SRR4302535.258980/94‑1 (MQ=60)

                                CTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATG                                                               >  SRR4302535.1554481/1‑94 (MQ=60)

                                CTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATG                                                               <  SRR4302535.1831067/94‑1 (MQ=60)

                                 CTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGG                                                              <  SRR4302535.1036258/94‑1 (MQ=60)

                                  TCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGC                                                             <  SRR4302535.3785169/94‑1 (MQ=60)

                                    GACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGA                                                           >  SRR4302535.948968/1‑94 (MQ=60)

                                     ACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAG                                                          >  SRR4302535.780982/1‑94 (MQ=60)

                                     ACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAG                                                          >  SRR4302535.2227265/1‑94 (MQ=60)

                                       AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGC                                                        >  SRR4302535.1282463/1‑94 (MQ=60)

                                       AATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGACGTGCTCCCCATTCCGTTCTCATGGCGCGGC                                                        >  SRR4302535.886246/1‑94 (MQ=60)

                                        ATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCA                                                       <  SRR4302535.4574232/94‑1 (MQ=60)

                                        ATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCA                                                       >  SRR4302535.313692/1‑94 (MQ=60)

                                          AACCCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAAC                                                     <  SRR4302535.777230/94‑1 (MQ=60)

                                          AAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAAC                                                     >  SRR4302535.934229/1‑94 (MQ=60)

                                          AAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAAC                                                     >  SRR4302535.1713675/1‑94 (MQ=60)

                                           AACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACA                                                    >  SRR4302535.3112787/1‑94 (MQ=60)

                                            ACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAT                                                   <  SRR4302535.943334/94‑1 (MQ=60)

                                            ACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACAT                                                   <  SRR4302535.750731/94‑1 (MQ=60)

                                             CCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATT                                                  >  SRR4302535.4038128/1‑94 (MQ=60)

                                              CCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTT                                                 <  SRR4302535.1017927/94‑1 (MQ=60)

                                              CCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTT                                                 <  SRR4302535.3995141/94‑1 (MQ=60)

                                               CGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTC                                                <  SRR4302535.3818752/94‑1 (MQ=60)

                                               CGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTC                                                <  SRR4302535.4521379/94‑1 (MQ=60)

                                                  CGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGG                                             <  SRR4302535.1896907/94‑1 (MQ=60)

                                                   GCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGA                                            >  SRR4302535.2826907/1‑94 (MQ=60)

                                                   GCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGA                                            <  SRR4302535.1568237/94‑1 (MQ=60)

                                                   GCCAACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGA                                            >  SRR4302535.744259/1‑94 (MQ=60)

                                                    CCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAT                                           >  SRR4302535.1239090/1‑94 (MQ=60)

                                                    CCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGAT                                           <  SRR4302535.1420284/94‑1 (MQ=60)

                                                     CGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATG                                          >  SRR4302535.1049275/1‑94 (MQ=60)

                                                       ACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGA                                        <  SRR4302535.4494262/94‑1 (MQ=60)

                                                       ACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGA                                        >  SRR4302535.1205089/1‑94 (MQ=60)

                                                        CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                       <  SRR4302535.4530827/94‑1 (MQ=60)

                                                        CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                       >  SRR4302535.2524471/1‑94 (MQ=60)

                                                        CAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAA                                       <  SRR4302535.904099/94‑1 (MQ=60)

                                                         AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATTCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      >  SRR4302535.324403/1‑94 (MQ=60)

                                                         AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      >  SRR4302535.2160632/1‑94 (MQ=60)

                                                         AGAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAG                                      <  SRR4302535.3331967/94‑1 (MQ=60)

                                                          GAGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGA                                     <  SRR4302535.1691131/94‑1 (MQ=60)

                                                           AGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAT                                    <  SRR4302535.4634426/94‑1 (MQ=60)

                                                           AGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAT                                    <  SRR4302535.273004/94‑1 (MQ=60)

                                                           AGTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGAT                                    >  SRR4302535.1026105/1‑94 (MQ=60)

                                                            GTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCGAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATA                                   <  SRR4302535.4665742/94‑1 (MQ=60)

                                                            GTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATA                                   <  SRR4302535.4056693/94‑1 (MQ=60)

                                                            GTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATA                                   <  SRR4302535.276660/94‑1 (MQ=60)

                                                            GTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATA                                   >  SRR4302535.456995/1‑94 (MQ=60)

                                                            GTAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATA                                   >  SRR4302535.456996/1‑94 (MQ=60)

                                                             TAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAA                                  <  SRR4302535.2737523/94‑1 (MQ=60)

                                                             TAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAA                                  <  SRR4302535.2026593/94‑1 (MQ=60)

                                                             TAATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAA                                  <  SRR4302535.4182601/94‑1 (MQ=60)

                                                              AATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 >  SRR4302535.2892709/1‑94 (MQ=60)

                                                              AATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 >  SRR4302535.3230907/1‑94 (MQ=60)

                                                              AATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 >  SRR4302535.355170/1‑94 (MQ=60)

                                                              AATAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAAT                                 <  SRR4302535.1056248/94‑1 (MQ=60)

                                                                TAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAGCATTTCGGGATGGAAGATAATGT                               <  SRR4302535.4312942/94‑1 (MQ=60)

                                                                TAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGT                               <  SRR4302535.27190/94‑1 (MQ=60)

                                                                TAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGT                               <  SRR4302535.2938771/94‑1 (MQ=60)

                                                                TAGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGT                               >  SRR4302535.3545145/1‑94 (MQ=60)

                                                                TAGGTTTGACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGT                               <  SRR4302535.1553345/94‑1 (MQ=60)

                                                                 AGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                              <  SRR4302535.120585/94‑1 (MQ=60)

                                                                 AGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                              <  SRR4302535.3737065/94‑1 (MQ=60)

                                                                 AGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                              >  SRR4302535.65755/1‑94 (MQ=60)

                                                                 AGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                              <  SRR4302535.728051/94‑1 (MQ=60)

                                                                 AGGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTT                              <  SRR4302535.1906816/94‑1 (MQ=60)

                                                                  GGTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTC                             >  SRR4302535.1254213/1‑94 (MQ=60)

                                                                   GTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCT                            <  SRR4302535.2014418/94‑1 (MQ=60)

                                                                   GTTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCT                            >  SRR4302535.170619/1‑94 (MQ=60)

                                                                    TTTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTT                           <  SRR4302535.1150047/94‑1 (MQ=60)

                                                                     TTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTT                          <  SRR4302535.821806/94‑1 (MQ=60)

                                                                     TTTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTT                          <  SRR4302535.1598143/94‑1 (MQ=60)

                                                                      TTACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTG                         <  SRR4302535.1097652/94‑1 (MQ=60)

                                                                       TACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGC                        >  SRR4302535.3307369/1‑94 (MQ=60)

                                                                        ACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCT                       >  SRR4302535.4364380/1‑94 (MQ=60)

                                                                        ACTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCT                       >  SRR4302535.2656835/1‑94 (MQ=60)

                                                                         CTTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTA                      >  SRR4302535.2916628/1‑94 (MQ=60)

                                                                          TTAATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTAC                     >  SRR4302535.1328907/1‑94 (MQ=60)

                                                                            AATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAG                   <  SRR4302535.4018503/94‑1 (MQ=60)

                                                                            AATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAG                   <  SRR4302535.2672949/94‑1 (MQ=60)

                                                                             ATAGCTCTTCCTGTCCC‑TTCCAGGGAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGG                  <  SRR4302535.4519562/94‑1 (MQ=60)

                                                                             ATAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGG                  <  SRR4302535.2497786/94‑1 (MQ=60)

                                                                              TAGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGA                 >  SRR4302535.1791868/1‑94 (MQ=60)

                                                                               AGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAA                >  SRR4302535.3046707/1‑94 (MQ=60)

                                                                               AGCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAA                <  SRR4302535.2486133/94‑1 (MQ=60)

                                                                                GCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAA               >  SRR4302535.136917/1‑94 (MQ=60)

                                                                                GCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAA               >  SRR4302535.1074075/1‑94 (MQ=60)

                                                                                GCTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAA               >  SRR4302535.3579365/1‑94 (MQ=60)

                                                                                 CTCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAA              >  SRR4302535.8533/1‑94 (MQ=60)

                                                                                  TCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAT             >  SRR4302535.1082100/1‑94 (MQ=60)

                                                                                  TCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAT             <  SRR4302535.782947/94‑1 (MQ=60)

                                                                                  TCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAT             >  SRR4302535.1964417/1‑94 (MQ=60)

                                                                                  TCTTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAAT             <  SRR4302535.1241689/94‑1 (MQ=60)

                                                                                    ttcctttcCC‑TTCTAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATTGAAGATAATGTTCTTTGCTACAGGAAAATCA           <  SRR4302535.2432584/86‑1 (MQ=60)

                                                                                    TTCCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCA           >  SRR4302535.4544501/1‑94 (MQ=60)

                                                                                      CCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         <  SRR4302535.598862/94‑1 (MQ=60)

                                                                                      CCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         >  SRR4302535.1964238/1‑94 (MQ=60)

                                                                                      CCTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAAC         >  SRR4302535.4126592/1‑94 (MQ=60)

                                                                                       CTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        <  SRR4302535.29566/94‑1 (MQ=60)

                                                                                       CTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        <  SRR4302535.3934668/94‑1 (MQ=60)

                                                                                       CTGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACA        <  SRR4302535.2146650/94‑1 (MQ=60)

                                                                                        TGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA       >  SRR4302535.1911913/1‑94 (MQ=60)

                                                                                        TGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA       <  SRR4302535.687601/94‑1 (MQ=60)

                                                                                        TGTCCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAA       >  SRR4302535.1151669/1‑94 (MQ=60)

                                                                                         gtcCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAAT      <  SRR4302535.4449244/91‑1 (MQ=60)

                                                                                          tcCC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATA     <  SRR4302535.4494082/92‑1 (MQ=60)

                                                                                            CG‑TTCCAGGCAGTGATCCGCATGCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG   <  SRR4302535.743319/94‑1 (MQ=60)

                                                                                            CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG   >  SRR4302535.3787049/1‑94 (MQ=60)

                                                                                            CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATG   >  SRR4302535.171522/1‑94 (MQ=60)

                                                                                             C‑TTCTAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  SRR4302535.2749438/1‑94 (MQ=60)

                                                                                             C‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  SRR4302535.656757/1‑94 (MQ=60)

                                                                                             C‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  SRR4302535.3876766/1‑94 (MQ=60)

                                                                                             C‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  <  SRR4302535.2671357/94‑1 (MQ=60)

TTCGACATCGTGCGACAGGCCGAAGTGTTCTTCTTCGACAATAAACCCGCCGCCGACAGAGTAATAGGTTTTACTTAATAGCTCTTCCTGT‑CC‑TTCCAGGCAGTGATCCGCATTCCGTTCTCATGGCGAGGCAACATTTCGGGATGGAAGATAATGTTCTTTGCTACAGGAAAATCAACAATATGC  >  NC_000913/3256801‑3256986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`