Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,277,510 T→C V319A (GTG→GCG)  agaZ → putative tagatose‑6‑phosphate kinase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,5100TC99.5% 632.0 / ‑5.1 202V319A (GTG→GCG) agaZputative tagatose‑6‑phosphate kinase 2
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (120/81);  total (120/82)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 210 total aligned reads displayed.

TGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGC  >  NC_000913/3277420‑3277601
                                                                                          |                                                                                           
tGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGgcgc                                                                                           >  1:1012899/1‑93 (MQ=255)
 gACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCt                                                                                          >  2:2866318/1‑93 (MQ=255)
    cTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGa                                                                                       <  1:4179949/93‑1 (MQ=255)
    cTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGa                                                                                       <  1:2911232/93‑1 (MQ=255)
     ttCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGaa                                                                                      >  2:1712018/1‑93 (MQ=255)
     ttCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGaa                                                                                      >  2:2266287/1‑93 (MQ=255)
     ttCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGaa                                                                                      <  1:2817048/93‑1 (MQ=255)
        gCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGAc                                                                                   >  2:1663953/1‑93 (MQ=255)
        gCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGAc                                                                                   <  1:183666/93‑1 (MQ=255)
        gCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGAc                                                                                   <  1:625891/93‑1 (MQ=255)
          ccTGCGTGAAGCTCTGTTCTCTCTGGAGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGt                                                                                 >  2:4009175/1‑93 (MQ=255)
           cTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTg                                                                                <  2:4317918/93‑1 (MQ=255)
           cTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTg                                                                                >  2:145605/1‑93 (MQ=255)
           cTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAAATGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTg                                                                                >  2:2368521/1‑93 (MQ=255)
            tGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGa                                                                               >  1:1349821/1‑93 (MQ=255)
            tGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGa                                                                               <  2:3148680/93‑1 (MQ=255)
               ctGAAGCTCTGTGCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGc                                                                            <  1:4502791/92‑1 (MQ=255)
                tGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCt                                                                           <  1:143389/93‑1 (MQ=255)
                 gAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAATGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTc                                                                          <  1:2506742/93‑1 (MQ=255)
                   aGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGa                                                                        <  2:4145613/93‑1 (MQ=255)
                   aGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGa                                                                        >  1:1210426/1‑93 (MQ=255)
                    gCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGGGCTGGAAGACGTGGTGCTCGAc                                                                       >  2:1954799/1‑93 (MQ=255)
                    gCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGAc                                                                       <  2:3877509/93‑1 (MQ=255)
                      tctGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCg                                                                     >  1:3577740/1‑93 (MQ=255)
                      tctGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCg                                                                     >  1:4684664/1‑93 (MQ=255)
                      tctGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGCGATGCTCGAccc                                                                     >  2:2702893/1‑92 (MQ=255)
                       ctGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGc                                                                    >  1:2252662/1‑93 (MQ=255)
                       ctGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGc                                                                    >  1:1914055/1‑93 (MQ=255)
                        tGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGcc                                                                   >  2:1110007/1‑93 (MQ=255)
                        tGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGcc                                                                   <  2:940150/93‑1 (MQ=255)
                        tGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGcc                                                                   <  1:1655388/93‑1 (MQ=255)
                        tGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGcc                                                                   >  2:772442/1‑93 (MQ=255)
                          ttCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCg                                                                 >  2:3656367/1‑93 (MQ=255)
                           tctctcTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGCCGGCCCgg                                                                >  1:3862048/1‑93 (MQ=255)
                           tctctcTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCgg                                                                >  2:719965/1‑93 (MQ=255)
                             tctctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGaa                                                              >  2:2406947/1‑93 (MQ=255)
                             tctctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGaa                                                              <  2:4194041/93‑1 (MQ=255)
                              ctgtggtggCGATTGAAGAAGAACTGGTGCTAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAAt                                                             <  1:751253/90‑1 (MQ=255)
                                ctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATAc                                                           <  1:2549809/93‑1 (MQ=255)
                                  ggcggcGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTg                                                         <  1:838849/93‑1 (MQ=255)
                                  ggcggcGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTg                                                         >  2:1410373/1‑93 (MQ=255)
                                   gcggcgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTgg                                                        >  2:546394/1‑93 (MQ=255)
                                       cgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCaaa                                                    >  2:2537923/1‑93 (MQ=255)
                                          ttGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGcc                                                 <  1:3412868/93‑1 (MQ=255)
                                          ttGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGcc                                                 >  2:596735/1‑93 (MQ=255)
                                           tGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCa                                                >  2:2921768/1‑93 (MQ=255)
                                              agaagaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTa                                             >  2:2765145/1‑93 (MQ=255)
                                              agaagaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTa                                             <  2:918225/93‑1 (MQ=255)
                                              agaagaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTa                                             >  1:969385/1‑93 (MQ=255)
                                              agaagaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTa                                             <  2:3831503/93‑1 (MQ=255)
                                               gaagaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTAc                                            >  2:1760429/1‑93 (MQ=255)
                                                aagaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTAcc                                           <  1:3393302/93‑1 (MQ=255)
                                                aagaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTAcc                                           <  2:1820522/93‑1 (MQ=255)
                                                  gaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAAGACTGGCAAAGCCACTACCAc                                         <  2:3507239/93‑1 (MQ=255)
                                                    aCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACgg                                       >  1:4447890/1‑93 (MQ=255)
                                                    aCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACgg                                       <  1:2407983/93‑1 (MQ=255)
                                                     cTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGt                                      <  1:305633/93‑1 (MQ=255)
                                                     cTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGt                                      <  2:3094781/93‑1 (MQ=255)
                                                     cTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGt                                      <  2:3026805/93‑1 (MQ=255)
                                                      tGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTg                                     <  1:2100306/93‑1 (MQ=255)
                                                        gTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGAc                                   <  2:1600856/93‑1 (MQ=255)
                                                        gTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGAc                                   <  1:1506066/93‑1 (MQ=255)
                                                        gTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGAc                                   >  1:1118961/1‑93 (MQ=255)
                                                         tGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACg                                  >  2:3424018/1‑93 (MQ=255)
                                                         tGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACg                                  <  1:1326008/93‑1 (MQ=255)
                                                           ccAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGc                                >  1:2033258/1‑93 (MQ=255)
                                                            cAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCa                               >  2:506468/1‑93 (MQ=255)
                                                             aGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCaa                              <  1:4564448/93‑1 (MQ=255)
                                                              gCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAc                             <  2:1495821/93‑1 (MQ=255)
                                                               cGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcg                            >  1:3342282/1‑93 (MQ=255)
                                                                gAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgc                           >  2:4221697/1‑93 (MQ=255)
                                                                gAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgc                           >  1:2833099/1‑93 (MQ=255)
                                                                gAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgc                           >  1:3197244/1‑93 (MQ=255)
                                                                gAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgc                           <  2:1924256/93‑1 (MQ=255)
                                                                 aaaGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgcg                          >  2:4053666/1‑93 (MQ=255)
                                                                 aaaGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgcg                          >  1:3073578/1‑93 (MQ=255)
                                                                  aaGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgcgc                         <  2:245779/93‑1 (MQ=255)
                                                                     ccTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGcgtc                      >  1:3670781/1‑93 (MQ=255)
                                                                      cTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGcgtcg                     >  1:3006237/1‑93 (MQ=255)
                                                                       tGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTGCCACGGTGACGGCAACGCGcgtcgc                    <  2:4450398/93‑2 (MQ=255)
                                                                       tGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGcgtcgt                    >  2:2339062/1‑93 (MQ=255)
                                                                        gTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCgtcgtc                   >  1:2321642/1‑93 (MQ=255)
                                                                        gTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCgtcgtc                   <  1:3265445/93‑1 (MQ=255)
                                                                        gTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCgtcgtc                   <  1:1591819/93‑1 (MQ=255)
                                                                        gTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCgtcgtc                   <  2:2482780/93‑1 (MQ=255)
                                                                          tCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTg                 >  2:3300866/1‑93 (MQ=255)
                                                                           cTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTgg                <  2:2043017/93‑1 (MQ=255)
                                                                            tGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgc               >  1:2703846/1‑93 (MQ=255)
                                                                             ggTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcg              <  2:21222/93‑1 (MQ=255)
                                                                              gTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc             <  1:3595214/93‑1 (MQ=255)
                                                                              gTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc             >  2:1264603/1‑93 (MQ=255)
                                                                                  tcGTCAGGCGCTGGAAGACGTGATGATCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCACCGCGCGTCGCCTGGCGCGTgg         <  2:1747639/92‑1 (MQ=255)
                                                                                     tCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTta      >  1:2950131/1‑93 (MQ=255)
                                                                                     tCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTta      >  1:1972/1‑93 (MQ=255)
                                                                                     ccAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTta      <  1:4084243/92‑1 (MQ=255)
                                                                                      cAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACAGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTtat     <  2:4287066/93‑1 (MQ=255)
                                                                                       aGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTtata    <  1:2016278/93‑1 (MQ=255)
                                                                                       aGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTtata    >  1:1260625/1‑93 (MQ=255)
                                                                                       aGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTtata    <  2:3984567/93‑1 (MQ=255)
                                                                                         gTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGc  <  2:2610285/93‑1 (MQ=255)
                                                                                          |                                                                                           
TGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGC  >  NC_000913/3277420‑3277601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTA  >  NC_000913/3277417‑3277603
                                                                                             |                                                                                             
CGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGC                                                                                               >  SRR4302535.1920229/1‑94 (MQ=60)
CGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGC                                                                                               >  SRR4302535.355802/1‑94 (MQ=60)
 GCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCG                                                                                              >  SRR4302535.194796/1‑94 (MQ=60)
  CTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGC                                                                                             >  SRR4302535.1020101/1‑94 (MQ=60)
     ACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGGAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGG                                                                                          >  SRR4302535.2534922/1‑94 (MQ=60)
     ACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGG                                                                                          >  SRR4302535.2036232/1‑94 (MQ=60)
       CTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAA                                                                                        <  SRR4302535.4208556/94‑1 (MQ=60)
       CTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAA                                                                                        <  SRR4302535.2931795/94‑1 (MQ=60)
        TTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAG                                                                                       <  SRR4302535.2837056/94‑1 (MQ=60)
        TTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAG                                                                                       >  SRR4302535.3266921/1‑94 (MQ=60)
        TTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGACTGTTCTGGTCTGCGTCAGGCGCTGGAAG                                                                                       >  SRR4302535.541540/1‑94 (MQ=60)
           GCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACG                                                                                    <  SRR4302535.630243/94‑1 (MQ=60)
           GCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACG                                                                                    <  SRR4302535.4019144/94‑1 (MQ=60)
           GCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACG                                                                                    <  SRR4302535.184930/94‑1 (MQ=60)
            CCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGT                                                                                   >  SRR4302535.3340635/1‑94 (MQ=60)
              CTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGA                                                                                 >  SRR4302535.984092/1‑94 (MQ=60)
              CTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGA                                                                                 >  SRR4302535.1359460/1‑94 (MQ=60)
                GCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATG                                                                               <  SRR4302535.1708794/94‑1 (MQ=60)
                  CTGAAGCTCTGTGCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCT                                                                             <  SRR4302535.4533710/94‑1 (MQ=60)
                   TGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTC                                                                            <  SRR4302535.144387/94‑1 (MQ=60)
                    GAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAATGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCG                                                                           <  SRR4302535.2524814/94‑1 (MQ=60)
                     AAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGA                                                                          >  SRR4302535.1219121/1‑94 (MQ=60)
                      AGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGAC                                                                         <  SRR4302535.2651751/94‑1 (MQ=60)
                        CTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCG                                                                       >  SRR4302535.3602543/1‑94 (MQ=60)
                        CTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCG                                                                       >  SRR4302535.4716362/1‑94 (MQ=60)
                         TCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGC                                                                      >  SRR4302535.2268702/1‑94 (MQ=60)
                         TCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGC                                                                      >  SRR4302535.1927849/1‑94 (MQ=60)
                           TGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCC                                                                    <  SRR4302535.1667361/94‑1 (MQ=60)
                            GTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCG                                                                   >  SRR4302535.2838682/1‑94 (MQ=60)
                            GTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCG                                                                   >  SRR4302535.1405902/1‑94 (MQ=60)
                             TTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGCCGGCCCGG                                                                  >  SRR4302535.3888621/1‑94 (MQ=60)
                               CTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAA                                                                >  SRR4302535.2911171/1‑94 (MQ=60)
                               CTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAA                                                                >  SRR4302535.3434393/1‑94 (MQ=60)
                                TCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAAT                                                               >  SRR4302535.295965/1‑94 (MQ=60)
                                 CTGTGGTGGCGATTGAAGAAGAACTGGTGCTAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATA                                                              <  SRR4302535.756545/94‑1 (MQ=60)
                                 CTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATA                                                              <  SRR4302535.2279800/94‑1 (MQ=60)
                                   CTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACT                                                            <  SRR4302535.2568165/94‑1 (MQ=60)
                                    TGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCGTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTG                                                           <  SRR4302535.2175295/94‑1 (MQ=60)
                                    TGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTG                                                           <  SRR4302535.417733/94‑1 (MQ=60)
                                     GGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGG                                                          <  SRR4302535.844740/94‑1 (MQ=60)
                                     GGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGG                                                          <  SRR4302535.3001546/94‑1 (MQ=60)
                                        GGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAA                                                       >  SRR4302535.3369069/1‑94 (MQ=60)
                                        GGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAA                                                       >  SRR4302535.1142826/1‑94 (MQ=60)
                                          CGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAG                                                     <  SRR4302535.4228406/94‑1 (MQ=60)
                                          CGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAG                                                     >  SRR4302535.1972855/1‑94 (MQ=60)
                                          CGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAG                                                     >  SRR4302535.77675/1‑94 (MQ=60)
                                           GATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGC                                                    >  SRR4302535.2344914/1‑94 (MQ=60)
                                           GATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGC                                                    >  SRR4302535.3817134/1‑94 (MQ=60)
                                             TTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCA                                                  <  SRR4302535.3436615/94‑1 (MQ=60)
                                              TGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCAC                                                 >  SRR4302535.4314981/1‑94 (MQ=60)
                                              TGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCAC                                                 <  SRR4302535.4615721/94‑1 (MQ=60)
                                                AAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTA                                               >  SRR4302535.976270/1‑94 (MQ=60)
                                                AAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTA                                               >  SRR4302535.3589632/1‑94 (MQ=60)
                                                 AGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTAC                                              >  SRR4302535.2553319/1‑94 (MQ=60)
                                                  GAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACC                                             <  SRR4302535.1920066/94‑1 (MQ=60)
                                                  GAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACC                                             <  SRR4302535.4497851/94‑1 (MQ=60)
                                                   AAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCA                                            <  SRR4302535.3416911/94‑1 (MQ=60)
                                                    AGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCAC                                           >  SRR4302535.1802912/1‑94 (MQ=60)
                                                     GAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACG                                          >  SRR4302535.1601003/1‑94 (MQ=60)
                                                     GAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACG                                          <  SRR4302535.1307762/94‑1 (MQ=60)
                                                      AACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGG                                         >  SRR4302535.4478390/1‑94 (MQ=60)
                                                      AACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGG                                         >  SRR4302535.2083273/1‑94 (MQ=60)
                                                       ACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGT                                        <  SRR4302535.2425318/94‑1 (MQ=60)
                                                        CTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTG                                       >  SRR4302535.2817859/1‑94 (MQ=60)
                                                        CTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTG                                       <  SRR4302535.307751/94‑1 (MQ=60)
                                                        CTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTG                                       <  SRR4302535.2446211/94‑1 (MQ=60)
                                                         TGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGA                                      <  SRR4302535.2115453/94‑1 (MQ=60)
                                                         TGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGA                                      >  SRR4302535.4576821/1‑94 (MQ=60)
                                                          GGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGAC                                     >  SRR4302535.1126944/1‑94 (MQ=60)
                                                           GTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACG                                    <  SRR4302535.1516894/94‑1 (MQ=60)
                                                           GTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACG                                    <  SRR4302535.3678989/94‑1 (MQ=60)
                                                            TGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGG                                   >  SRR4302535.3081917/1‑94 (MQ=60)
                                                            TGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGG                                   <  SRR4302535.1335473/94‑1 (MQ=60)
                                                             GCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGC                                  >  SRR4302535.2047910/1‑94 (MQ=60)
                                                              CCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCA                                 <  SRR4302535.888610/94‑1 (MQ=60)
                                                                AGCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAC                               <  SRR4302535.4595594/94‑1 (MQ=60)
                                                                 GCGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACG                              >  SRR4302535.3365571/1‑94 (MQ=60)
                                                                  CGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGC                             >  SRR4302535.1339620/1‑94 (MQ=60)
                                                                  CGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGC                             >  SRR4302535.2853208/1‑94 (MQ=60)
                                                                  CGAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGC                             >  SRR4302535.3219368/1‑94 (MQ=60)
                                                                   GAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCG                            >  SRR4302535.3095032/1‑94 (MQ=60)
                                                                   GAAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCG                            <  SRR4302535.3057967/94‑1 (MQ=60)
                                                                    AAAGCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAATCCACTACCACGGTGACGGCAACGCGC                           >  SRR4302535.3602560/1‑94 (MQ=60)
                                                                       GCCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTC                        >  SRR4302535.3696201/1‑94 (MQ=60)
                                                                        CCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCG                       >  SRR4302535.3027300/1‑94 (MQ=60)
                                                                        CCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCG                       >  SRR4302535.1240638/1‑94 (MQ=60)
                                                                        CCTGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTAG                       >  SRR4302535.1666964/1‑94 (MQ=60)
                                                                          TGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTC                     >  SRR4302535.2338257/1‑94 (MQ=60)
                                                                          TGTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTC                     <  SRR4302535.1020832/94‑1 (MQ=60)
                                                                           GTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCT                    <  SRR4302535.1603303/94‑1 (MQ=60)
                                                                           GTTCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCT                    <  SRR4302535.3288125/94‑1 (MQ=60)
                                                                             TCTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGG                  <  SRR4302535.3238163/94‑1 (MQ=60)
                                                                              CTGGTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGC                 >  SRR4302535.2723163/1‑94 (MQ=60)
                                                                                 GTCTGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCG              <  SRR4302535.3620163/94‑1 (MQ=60)
                                                                                    TGCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGCATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGG           >  SRR4302535.1473751/1‑94 (MQ=60)
                                                                                     GCGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGT          >  SRR4302535.7553/1‑94 (MQ=60)
                                                                                      CGTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTT         >  SRR4302535.2006298/1‑94 (MQ=60)
                                                                                       GTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTA        >  SRR4302535.2970857/1‑94 (MQ=60)
                                                                                       GTCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTA        >  SRR4302535.1991/1‑94 (MQ=60)
                                                                                        TCAGGCGCTGGAGGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTAT       <  SRR4302535.3099771/94‑1 (MQ=60)
                                                                                        CCAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTAT       <  SRR4302535.4112170/94‑1 (MQ=60)
                                                                                         CAGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATA      >  SRR4302535.1269646/1‑94 (MQ=60)
                                                                                          AGGCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAG     <  SRR4302535.2030804/94‑1 (MQ=60)
                                                                                            GCGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCT   <  SRR4302535.557675/94‑1 (MQ=60)
                                                                                             CGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTA  >  SRR4302535.2849432/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTA  >  NC_000913/3277417‑3277603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑