Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,327,704 (C)5→6 coding (1101/1434 nt) dacB → D‑alanyl‑D‑alanine carboxypeptidase fraction B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,327,6991.C98.1% 659.5 / 4.1 211coding (1096/1434 nt)dacBD‑alanyl‑D‑alanine carboxypeptidase fraction B
Reads supporting (aligned to +/- strand):  ref base . (4/0);  new base C (103/104);  total (107/104)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

CCGTGCGTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTG‑CC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTAT  >  NC_000913/3327607‑3327788
                                                                                              |                                                                                           
ccGTGCGTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTg                                                                                              <  1:333514/93‑1 (MQ=255)
ccGTGCGTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTg                                                                                              >  2:1028166/1‑93 (MQ=255)
 cGTGCGTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTG‑c                                                                                            <  1:3965071/93‑1 (MQ=255)
   tGCGTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTG‑cgc                                                                                          >  1:2969681/1‑91 (MQ=255)
   tGCGTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTG‑cc‑c                                                                                         >  2:74689/1‑93 (MQ=255)
     cGTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTG‑cc‑c‑cc                                                                                      >  2:3693304/1‑93 (MQ=255)
       tCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTG‑cc‑c‑cccg                                                                                    >  1:3410061/1‑91 (MQ=255)
         aGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTG‑cc‑c‑cccgcc                                                                                  >  1:2932485/1‑89 (MQ=255)
           aTCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGccac                                                                                 >  1:4162711/1‑93 (MQ=255)
             ccTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGccacca                                                                               <  2:3933989/93‑1 (MQ=255)
             ccTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGccacca                                                                               >  1:841789/1‑93 (MQ=255)
              cTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCat                                                                              >  1:578318/1‑93 (MQ=255)
              cTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCat                                                                              >  2:2991518/1‑93 (MQ=255)
              cTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCat                                                                              >  1:3006665/1‑93 (MQ=255)
               tGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCatg                                                                             >  1:1342930/1‑93 (MQ=255)
               tGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCatg                                                                             >  2:3430295/1‑93 (MQ=255)
                gcgcCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCatga                                                                            <  2:1128694/93‑1 (MQ=255)
                gcgcCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCatga                                                                            <  1:3115100/93‑1 (MQ=255)
                 cgcCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCatgat                                                                           <  2:2218977/93‑1 (MQ=255)
                   caaGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGc                                                                         <  1:234523/91‑1 (MQ=255)
                    cAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCa                                                                        <  2:2786370/93‑1 (MQ=255)
                     aGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAg                                                                       >  2:2129680/1‑93 (MQ=255)
                     aGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAg                                                                       <  1:3085541/93‑1 (MQ=255)
                     aGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAg                                                                       >  1:1390797/1‑93 (MQ=255)
                     aGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCAACATGATGCAg                                                                       >  2:548974/1‑93 (MQ=255)
                        aaGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGtg                                                                    <  1:4393101/93‑1 (MQ=255)
                         aGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGtgc                                                                   >  1:3239402/1‑93 (MQ=255)
                         aGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGtgc                                                                   <  2:835702/93‑1 (MQ=255)
                         aGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGtgc                                                                   >  1:1015142/1‑93 (MQ=255)
                         aGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCACGGCTTTCCCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAgtggt                                                                   >  1:2903237/1‑89 (MQ=255)
                            cGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGtgctgc                                                                <  1:4612429/93‑1 (MQ=255)
                            cGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCAGCATAACCTGA‑TTG‑CCAC‑CCACCACCATAGTGCAGGtgctgc                                                                >  1:1115037/1‑93 (MQ=255)
                              gtgtCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCaa                                                              <  1:4288871/93‑1 (MQ=255)
                              gtgtCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCaa                                                              >  2:3924391/1‑93 (MQ=255)
                                gtCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATa                                                            >  1:163394/1‑93 (MQ=255)
                                   gATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACAt                                                         >  1:533738/1‑93 (MQ=255)
                                    atatTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACAtt                                                        >  1:3283926/1‑93 (MQ=255)
                                    atatTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACAtt                                                        >  2:67374/1‑93 (MQ=255)
                                          gAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCaa                                                  >  1:3546120/1‑93 (MQ=255)
                                          gAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCaa                                                  <  2:2710169/93‑1 (MQ=255)
                                           aaaCACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAac                                                 >  1:1725989/1‑93 (MQ=255)
                                           aaaCACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAac                                                 <  1:223802/93‑1 (MQ=255)
                                           aaaCACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAac                                                 <  2:214212/93‑1 (MQ=255)
                                            aaCACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAaca                                                <  2:3177303/93‑1 (MQ=255)
                                             acacCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAacac                                               >  2:2813408/1‑93 (MQ=255)
                                             acacCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAacac                                               <  1:3805210/93‑1 (MQ=255)
                                             acacCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAacac                                               <  1:3907060/93‑1 (MQ=255)
                                                ccATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGAc                                            <  2:509733/93‑1 (MQ=255)
                                                ccATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGAc                                            >  1:2947065/1‑93 (MQ=255)
                                                 cATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACa                                           <  1:1996644/93‑1 (MQ=255)
                                                 cATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACa                                           <  1:687073/93‑1 (MQ=255)
                                                  attattGCCGGTGTTTCAGGGCTTGCGCGGCATAACCTTA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACaa                                          <  2:4052478/93‑1 (MQ=255)
                                                  attattGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACaa                                          >  1:1065413/1‑93 (MQ=255)
                                                  attattGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACaa                                          >  2:2431867/1‑93 (MQ=255)
                                                  attattGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACaa                                          <  1:3296893/93‑1 (MQ=255)
                                                   ttattGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAAt                                         <  1:4271965/93‑1 (MQ=255)
                                                   ttattGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAAt                                         <  2:769911/93‑1 (MQ=255)
                                                    tattGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATg                                        >  1:2675512/1‑93 (MQ=255)
                                                      ttGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaa                                      >  1:1499924/1‑93 (MQ=255)
                                                        gCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaact                                    <  1:1802685/93‑1 (MQ=255)
                                                         ccGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaactt                                   <  2:3063634/93‑1 (MQ=255)
                                                         ccGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaactt                                   >  2:871271/1‑93 (MQ=255)
                                                         ccGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaactt                                   <  1:361640/93‑1 (MQ=255)
                                                         ccGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaactt                                   >  1:3633140/1‑93 (MQ=255)
                                                          cGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaactta                                  >  2:1976330/1‑93 (MQ=255)
                                                          cGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaactta                                  >  2:1464612/1‑93 (MQ=255)
                                                           gATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaacttaa                                 <  1:254070/93‑1 (MQ=255)
                                                           gATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaacttaa                                 >  2:439163/1‑93 (MQ=255)
                                                           gATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaacttaa                                 <  2:1435127/93‑1 (MQ=255)
                                                            aTGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaacttaac                                <  1:2802007/93‑1 (MQ=255)
                                                            aTGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaacttaac                                <  1:2811099/93‑1 (MQ=255)
                                                             tGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaacttaact                               >  2:1687840/1‑93 (MQ=255)
                                                             tGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaacttaact                               <  1:1306767/93‑1 (MQ=255)
                                                              ggTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGaacttaactt                              >  1:1196965/1‑93 (MQ=255)
                                                               gTTCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACttt                             <  2:2010546/93‑1 (MQ=255)
                                                                ttCAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTa                            <  2:3343602/93‑1 (MQ=255)
                                                                 tCAGGGCTTGCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTAt                           <  2:1604970/93‑1 (MQ=255)
                                                                  cAGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTAtc                          <  1:3680471/93‑1 (MQ=255)
                                                                   aGGGCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTAtct                         >  2:1885686/1‑93 (MQ=255)
                                                                    gggCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTAtctc                        >  2:1625088/1‑93 (MQ=255)
                                                                    gggCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTAtctc                        >  2:3886108/1‑93 (MQ=255)
                                                                    gggCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTAtctc                        >  1:4666390/1‑93 (MQ=255)
                                                                      gCTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCa                      >  2:3754532/1‑93 (MQ=255)
                                                                       cTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAt                     <  2:1723021/93‑1 (MQ=255)
                                                                       cTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAt                     <  2:1478878/93‑1 (MQ=255)
                                                                       cTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAt                     <  1:964093/93‑1 (MQ=255)
                                                                       cTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAt                     <  2:43561/93‑1 (MQ=255)
                                                                       cTTTCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAt                     <  1:2128136/93‑1 (MQ=255)
                                                                        tttCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAtg                    <  1:4561019/93‑1 (MQ=255)
                                                                         ttCGCGGCATAACCTGATTTC‑CC‑C‑CCGGCACCATTATGCAGGGGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAtgc                   <  1:2093829/93‑1 (MQ=255)
                                                                          tCGCGGCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGGGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAtgct                  >  2:2817286/1‑93 (MQ=255)
                                                                               gCATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGTTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGc             <  1:483136/93‑1 (MQ=255)
                                                                                cATAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCt            >  1:2581404/1‑93 (MQ=255)
                                                                                  tAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTgg          >  1:2555863/1‑93 (MQ=255)
                                                                                  tAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTgg          >  1:2429133/1‑93 (MQ=255)
                                                                                  tAACCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTgg          <  1:2193756/93‑1 (MQ=255)
                                                                                  aaaCCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTgg          <  2:4248261/92‑1 (MQ=255)
                                                                                   aaCCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGc         >  2:328963/1‑93 (MQ=255)
                                                                                    aCCTGA‑TTGCCC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCg        <  2:3306944/93‑1 (MQ=255)
                                                                                           ttG‑CC‑CACCGCCACCATAATGCAGGTGCTGCAATACATTGCCCCACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTAt  >  2:2649437/1‑93 (MQ=255)
                                                                                              |                                                                                           
CCGTGCGTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTG‑CC‑C‑CCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTAT  >  NC_000913/3327607‑3327788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑G‑CC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTATGACGGCTCT  >  NC_000913/3327613‑3327797
                                                                                               |                                                                                             
GTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑G‑CC‑CCCcg                                                                                             >  SRR4302535.3433791/1‑92 (MQ=60)
  CAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCC                                                                                            >  SRR4302535.2953131/1‑94 (MQ=60)
    GATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCAC                                                                                          >  SRR4302535.4191205/1‑94 (MQ=60)
     ATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACC                                                                                         >  SRR4302535.2112789/1‑94 (MQ=60)
      TCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCA                                                                                        >  SRR4302535.847694/1‑94 (MQ=60)
       CCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCAT                                                                                       >  SRR4302535.582383/1‑94 (MQ=60)
       CCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCAT                                                                                       >  SRR4302535.3027728/1‑94 (MQ=60)
       CCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCAT                                                                                       <  SRR4302535.1637661/94‑1 (MQ=60)
        CTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATG                                                                                      <  SRR4302535.3319682/94‑1 (MQ=60)
        CTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATG                                                                                      >  SRR4302535.1352516/1‑94 (MQ=60)
         TGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGA                                                                                     >  SRR4302535.739959/1‑94 (MQ=60)
          GCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGAT                                                                                    <  SRR4302535.3136788/94‑1 (MQ=60)
           CCCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATG                                                                                   <  SRR4302535.3385624/94‑1 (MQ=60)
            GCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGC                                                                                  >  SRR4302535.2926580/1‑94 (MQ=60)
             CAAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCA                                                                                 <  SRR4302535.236144/94‑1 (MQ=60)
              CAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAG                                                                                >  SRR4302535.1400722/1‑94 (MQ=60)
              CAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAG                                                                                >  SRR4302535.58006/1‑94 (MQ=60)
              CAGAAAGCCGTTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAG                                                                                <  SRR4302535.2668837/94‑1 (MQ=60)
               AGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGG                                                                               <  SRR4302535.3107034/94‑1 (MQ=60)
                 CAAGCCGGTGTCGATATTGGAAACGCCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTG                                                                             <  SRR4302535.1113412/94‑1 (MQ=60)
                 CAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTG                                                                             >  SRR4302535.44150/1‑94 (MQ=60)
                  AAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGC                                                                            >  SRR4302535.3775468/1‑94 (MQ=60)
                  AAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGC                                                                            >  SRR4302535.1022361/1‑94 (MQ=60)
                  AAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGC                                                                            >  SRR4302535.2984526/1‑94 (MQ=60)
                  AAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGC                                                                            >  SRR4302535.3261895/1‑94 (MQ=60)
                  AAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGC                                                                            <  SRR4302535.4423160/94‑1 (MQ=60)
                  AAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGC                                                                            <  SRR4302535.854215/94‑1 (MQ=60)
                  AAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCACGGCTTTCCCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGtggt                                                                            >  SRR4302535.2923758/1‑90 (MQ=60)
                    GCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTG                                                                          <  SRR4302535.1043792/94‑1 (MQ=60)
                     CCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGC                                                                         <  SRR4302535.3445533/94‑1 (MQ=60)
                     CCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCAGCATAACCTGA‑TT‑G‑CCACCCACCACCATAGTGCAGGTGCTGC                                                                         >  SRR4302535.1122984/1‑94 (MQ=60)
                      CGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCA                                                                        <  SRR4302535.4643767/94‑1 (MQ=60)
                        GTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAAT                                                                      <  SRR4302535.4318208/94‑1 (MQ=60)
                         TGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TTAC‑CC‑CCCGCCACCATGATGCAGGTGCTGCAATA                                                                     >  SRR4302535.3307717/1‑94 (MQ=60)
                         TGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATA                                                                     <  SRR4302535.308819/94‑1 (MQ=60)
                         TGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATA                                                                     >  SRR4302535.164535/1‑94 (MQ=60)
                          GTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATAC                                                                    >  SRR4302535.2292605/1‑94 (MQ=60)
                          GTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATAC                                                                    <  SRR4302535.3107364/94‑1 (MQ=60)
                            CGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACAT                                                                  >  SRR4302535.537472/1‑94 (MQ=60)
                            CGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACAT                                                                  >  SRR4302535.3600693/1‑94 (MQ=60)
                             GATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATT                                                                 >  SRR4302535.3306733/1‑94 (MQ=60)
                               TATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGC                                                               >  SRR4302535.1373537/1‑94 (MQ=60)
                                ATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCC                                                              <  SRR4302535.247334/94‑1 (MQ=60)
                                   GGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAA                                                           >  SRR4302535.3570742/1‑94 (MQ=60)
                                    GAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAAC                                                          >  SRR4302535.1738469/1‑94 (MQ=60)
                                     AAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACA                                                         <  SRR4302535.225341/94‑1 (MQ=60)
                                     AAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACA                                                         >  SRR4302535.3066618/1‑94 (MQ=60)
                                     AAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACA                                                         <  SRR4302535.2936027/94‑1 (MQ=60)
                                       ACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACG                                                       >  SRR4302535.2179404/1‑94 (MQ=60)
                                       ACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACG                                                       <  SRR4302535.3831439/94‑1 (MQ=60)
                                       ACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACG                                                       <  SRR4302535.3933828/94‑1 (MQ=60)
                                        CACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGA                                                      <  SRR4302535.2993896/94‑1 (MQ=60)
                                         ACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGAC                                                     >  SRR4302535.3473577/1‑94 (MQ=60)
                                         ACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGAC                                                     <  SRR4302535.4306776/94‑1 (MQ=60)
                                         ACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGAC                                                     >  SRR4302535.2967773/1‑94 (MQ=60)
                                         ACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGAC                                                     <  SRR4302535.3261096/94‑1 (MQ=60)
                                          CCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACA                                                    >  SRR4302535.3766633/1‑94 (MQ=60)
                                          CCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACA                                                    >  SRR4302535.1840168/1‑94 (MQ=60)
                                          CCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACA                                                    <  SRR4302535.4692409/94‑1 (MQ=60)
                                           CATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAA                                                   >  SRR4302535.1072992/1‑94 (MQ=60)
                                           CATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAA                                                   <  SRR4302535.2011018/94‑1 (MQ=60)
                                           CATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAA                                                   <  SRR4302535.691886/94‑1 (MQ=60)
                                            ATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACACT                                                  >  SRR4302535.1287164/1‑94 (MQ=60)
                                            ATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAAT                                                  <  SRR4302535.3319813/94‑1 (MQ=60)
                                            ATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAAT                                                  >  SRR4302535.4507340/1‑94 (MQ=60)
                                            ATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAAT                                                  >  SRR4302535.493654/1‑94 (MQ=60)
                                            ATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAAT                                                  <  SRR4302535.1776540/94‑1 (MQ=60)
                                             TTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATG                                                 >  SRR4302535.2694655/1‑94 (MQ=60)
                                             TTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATG                                                 <  SRR4302535.4301210/94‑1 (MQ=60)
                                               ATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAA                                               >  SRR4302535.1510713/1‑94 (MQ=60)
                                                 TGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACT                                             >  SRR4302535.3268869/1‑94 (MQ=60)
                                                 TGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACT                                             >  SRR4302535.4256896/1‑94 (MQ=60)
                                                  GCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTT                                            >  SRR4302535.3658318/1‑94 (MQ=60)
                                                  GCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTT                                            <  SRR4302535.1815716/94‑1 (MQ=60)
                                                   CCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTA                                           >  SRR4302535.224191/1‑94 (MQ=60)
                                                   CCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTA                                           <  SRR4302535.364136/94‑1 (MQ=60)
                                                    CGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAC                                          >  SRR4302535.2580938/1‑94 (MQ=60)
                                                    CGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAA                                          >  SRR4302535.2230376/1‑94 (MQ=60)
                                                     GATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAAC                                         <  SRR4302535.255824/94‑1 (MQ=60)
                                                     GATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAAC                                         >  SRR4302535.4020679/1‑94 (MQ=60)
                                                      ATGGTTCAGGTCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACT                                        <  SRR4302535.2468632/94‑1 (MQ=60)
                                                      ATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACT                                        <  SRR4302535.2821936/94‑1 (MQ=60)
                                                      ATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACT                                        <  SRR4302535.2831083/94‑1 (MQ=60)
                                                       TGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTT                                       <  SRR4302535.1316098/94‑1 (MQ=60)
                                                       TGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTT                                       >  SRR4302535.1205536/1‑94 (MQ=60)
                                                        GGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTT                                      >  SRR4302535.3928335/1‑94 (MQ=60)
                                                            CAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCT                                  <  SRR4302535.3705943/94‑1 (MQ=60)
                                                            CAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCT                                  >  SRR4302535.3684041/1‑94 (MQ=60)
                                                            CAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCT                                  >  SRR4302535.1159926/1‑94 (MQ=60)
                                                            CAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCT                                  >  SRR4302535.3965254/1‑94 (MQ=60)
                                                            CAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCT                                  <  SRR4302535.208360/94‑1 (MQ=60)
                                                            CAGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCT                                  <  SRR4302535.4600084/94‑1 (MQ=60)
                                                             AGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTC                                 >  SRR4302535.2410561/1‑94 (MQ=60)
                                                             AGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTC                                 >  SRR4302535.4697932/1‑94 (MQ=60)
                                                             AGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTC                                 <  SRR4302535.2715446/94‑1 (MQ=60)
                                                             AGGGCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTC                                 >  SRR4302535.1509923/1‑94 (MQ=60)
                                                                GCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAT                              <  SRR4302535.2824834/94‑1 (MQ=60)
                                                                GCTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCAT                              <  SRR4302535.2727192/94‑1 (MQ=60)
                                                                 CTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATG                             <  SRR4302535.970945/94‑1 (MQ=60)
                                                                 CTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATG                             <  SRR4302535.2143482/94‑1 (MQ=60)
                                                                 CTTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATG                             >  SRR4302535.3641586/1‑94 (MQ=60)
                                                                  TTTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGC                            <  SRR4302535.4592149/94‑1 (MQ=60)
                                                                   TTCGCGGCATAACCTGATTT‑C‑CC‑CCCGGCACCATTATGCAGGGGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCT                           <  SRR4302535.2108922/94‑1 (MQ=60)
                                                                   TTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCGGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCT                           <  SRR4302535.1827471/94‑1 (MQ=60)
                                                                   TTCGCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCT                           <  SRR4302535.4524366/94‑1 (MQ=60)
                                                                    TCGCGGCATACCCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTG                          <  SRR4302535.3332373/94‑1 (MQ=60)
                                                                      GCGGCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCC                        <  SRR4302535.2957452/94‑1 (MQ=60)
                                                                         GCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGTTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCT                     <  SRR4302535.486524/94‑1 (MQ=60)
                                                                         GCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCT                     >  SRR4302535.2599948/1‑94 (MQ=60)
                                                                         GCATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCT                     <  SRR4302535.486523/94‑1 (MQ=60)
                                                                          CATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTG                    >  SRR4302535.1667375/1‑94 (MQ=60)
                                                                           ATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGG                   >  SRR4302535.2900733/1‑94 (MQ=60)
                                                                           ATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGG                   >  SRR4302535.2574267/1‑94 (MQ=60)
                                                                           ATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGG                   >  SRR4302535.2446635/1‑94 (MQ=60)
                                                                           ATAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGG                   <  SRR4302535.1316559/94‑1 (MQ=60)
                                                                            TAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGC                  <  SRR4302535.795060/94‑1 (MQ=60)
                                                                            TAACCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGC                  <  SRR4302535.2209388/94‑1 (MQ=60)
                                                                               CCTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGG               >  SRR4302535.1579184/1‑94 (MQ=60)
                                                                                CTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGC              >  SRR4302535.1255825/1‑94 (MQ=60)
                                                                                CTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGC              <  SRR4302535.4116834/94‑1 (MQ=60)
                                                                                CTGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGC              >  SRR4302535.2559699/1‑94 (MQ=60)
                                                                                 TGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCT             <  SRR4302535.4714681/94‑1 (MQ=60)
                                                                                 TGA‑TT‑GCCC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCT             <  SRR4302535.664487/94‑1 (MQ=60)
                                                                                        C‑CC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATGTCCATGCTGCCGCTGGCGGGCTATGACG       >  SRR4302535.578678/1‑94 (MQ=60)
                                                                                        C‑CC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTATGACG       >  SRR4302535.4474650/1‑94 (MQ=60)
                                                                                          CC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTATGACGG      >  SRR4302535.4410899/1‑94 (MQ=60)
                                                                                          CC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTATGACGG      >  SRR4302535.3652943/1‑94 (MQ=60)
                                                                                          CC‑CCCGCCACCATGACGCAGGTGCTGCAATACATTGCCCAACACGACAATGACCTTAACTTTATCTCCATGCTGCCGCGGGCGGGCTATGACGG      >  SRR4302535.647630/1‑94 (MQ=60)
                                                                                             CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTATGACGGCT    <  SRR4302535.4141302/94‑1 (MQ=60)
                                                                                             CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTATGACGGCT    <  SRR4302535.4562725/94‑1 (MQ=60)
                                                                                               CGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTATGACGGCTCT  <  SRR4302535.928747/94‑1 (MQ=60)
                                                                                               |                                                                                             
GTCAGATCCTGCGCCAGCAAGCCGGTGTCGATATTGGAAACACCATTATTGCCGATGGTTCAGGGCTTTCGCGGCATAACCTGA‑TT‑G‑CC‑CCCGCCACCATGATGCAGGTGCTGCAATACATTGCCCAACACGACAATGAACTTAACTTTATCTCCATGCTGCCGCTGGCGGGCTATGACGGCTCT  >  NC_000913/3327613‑3327797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑