Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,333,953 A→G G61G (GGT→GGC)  murA ← UDP‑N‑glucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,333,9530AG99.5% 613.8 / ‑4.9 196G61G (GGT→GGC) murAUDP‑N‑glucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (110/85);  total (111/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 203 total aligned reads displayed.

ACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGC  >  NC_000913/3333863‑3334042
                                                                                          |                                                                                         
aCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAGTATGCACAGAACCATTACGTTCTACTTTCGCGcc                                                                                         >  2:3903094/1‑93 (MQ=255)
aCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGcc                                                                                         <  2:3752581/93‑1 (MQ=255)
aCGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGcc                                                                                         >  2:4185281/1‑93 (MQ=255)
 cGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGccc                                                                                        >  2:2373723/1‑93 (MQ=255)
  gCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCa                                                                                       >  1:777568/1‑93 (MQ=255)
  gCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCa                                                                                       >  2:1516820/1‑93 (MQ=255)
   cATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTATGTTCTACTTTCGCGCCCAg                                                                                      >  2:732725/1‑93 (MQ=255)
    aTGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGc                                                                                     >  2:1064669/1‑93 (MQ=255)
    aTGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGc                                                                                     <  1:1545498/93‑1 (MQ=255)
     tGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCt                                                                                    >  2:2057846/1‑93 (MQ=255)
     tGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCt                                                                                    >  2:2726520/1‑93 (MQ=255)
     tGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCt                                                                                    >  2:3318179/1‑93 (MQ=255)
      ggTTTTAACGAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTg                                                                                   <  2:4094988/93‑1 (MQ=255)
      ggTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTg                                                                                   <  1:1408464/93‑1 (MQ=255)
        ttttAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGc                                                                                 >  1:854709/1‑93 (MQ=255)
          ttAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCtt                                                                               >  2:1875632/1‑93 (MQ=255)
          ttAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCtt                                                                               <  1:2978811/93‑1 (MQ=255)
             aCCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTagc                                                                            <  1:1584303/93‑1 (MQ=255)
                 gATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCt                                                                        <  1:4295841/93‑1 (MQ=255)
                 gATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTCGCTTAGCAGCt                                                                        >  2:2951856/1‑93 (MQ=255)
                  aTCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCtt                                                                       >  1:2605172/1‑93 (MQ=255)
                     gTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCAt                                                                    >  1:962573/1‑93 (MQ=255)
                      tAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCAtt                                                                   <  1:3900884/93‑1 (MQ=255)
                      tAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCAtt                                                                   >  1:4234530/1‑93 (MQ=255)
                      tAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCAtt                                                                   >  2:1122128/1‑93 (MQ=255)
                      tAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCAtt                                                                   >  1:1146321/1‑93 (MQ=255)
                      tAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCAtt                                                                   >  2:4355323/1‑93 (MQ=255)
                       aaGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTg                                                                  <  2:3583039/93‑1 (MQ=255)
                        aGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGa                                                                 >  1:1891100/1‑93 (MQ=255)
                         ggTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGAt                                                                <  2:2408551/93‑1 (MQ=255)
                         ggTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGAt                                                                <  2:3821670/93‑1 (MQ=255)
                            gcgcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTa                                                             <  2:3267851/93‑1 (MQ=255)
                            gcgcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTa                                                             <  1:2056501/93‑1 (MQ=255)
                             cgcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTAt                                                            <  1:2285218/93‑1 (MQ=255)
                             cgcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTAt                                                            <  2:1179290/93‑1 (MQ=255)
                             cgcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTAt                                                            <  2:3977465/93‑1 (MQ=255)
                              gcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATc                                                           >  2:2708131/1‑93 (MQ=255)
                              gcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATc                                                           <  1:466620/93‑1 (MQ=255)
                                aGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGa                                                         <  2:3678532/93‑1 (MQ=255)
                                 gAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGAc                                                        >  2:2291596/1‑93 (MQ=255)
                                  aaTACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACg                                                       <  1:4034636/93‑1 (MQ=255)
                                   aTACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGt                                                      <  1:3442774/93‑1 (MQ=255)
                                    tACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTc                                                     <  2:1150470/93‑1 (MQ=255)
                                    tACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTc                                                     >  1:3555827/1‑93 (MQ=255)
                                      cATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCtt                                                   <  1:3006943/93‑1 (MQ=255)
                                      cATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCtt                                                   >  1:3194674/1‑93 (MQ=255)
                                      cATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCtt                                                   <  2:2618823/93‑1 (MQ=255)
                                      cATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCtt                                                   >  1:1016263/1‑93 (MQ=255)
                                      cATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCtt                                                   >  2:1835339/1‑93 (MQ=255)
                                       aTTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCttt                                                  >  1:480267/1‑93 (MQ=255)
                                        ttAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTTTTTc                                                 >  1:3722199/1‑93 (MQ=255)
                                        ttAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTc                                                 >  1:829882/1‑93 (MQ=255)
                                        ttAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTc                                                 >  2:1660459/1‑93 (MQ=255)
                                           aCGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGt                                              >  2:4316608/1‑93 (MQ=255)
                                            cGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGtt                                             >  2:4255916/1‑93 (MQ=255)
                                            cGTCGCGGCCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGtt                                             <  2:124363/93‑1 (MQ=255)
                                             ctCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGttt                                            <  1:3750432/92‑1 (MQ=255)
                                              tCGCGGGCATCAATATTCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTc                                           <  1:2102065/93‑1 (MQ=255)
                                               cgcgGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCg                                          >  1:680350/1‑93 (MQ=255)
                                                gcgGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCgg                                         >  2:1747700/1‑93 (MQ=255)
                                                  gggCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGa                                       >  1:93700/1‑93 (MQ=255)
                                                  gggCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGa                                       <  2:479109/93‑1 (MQ=255)
                                                   ggCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGAc                                      >  1:1979046/1‑93 (MQ=255)
                                                    gCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACg                                     >  1:4599668/1‑93 (MQ=255)
                                                    gCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACg                                     <  2:3798662/93‑1 (MQ=255)
                                                     cATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGt                                    <  1:3059145/93‑1 (MQ=255)
                                                     cATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGt                                    <  2:4549186/93‑1 (MQ=255)
                                                       tCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTc                                  >  2:3947769/1‑93 (MQ=255)
                                                        cAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCt                                 <  1:3029134/93‑1 (MQ=255)
                                                         aaTATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTg                                <  2:4444216/93‑1 (MQ=255)
                                                          atatGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTgg                               <  2:1752352/93‑1 (MQ=255)
                                                          atatGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTgg                               >  1:3157657/1‑93 (MQ=255)
                                                           tatGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGa                              >  1:1370287/1‑93 (MQ=255)
                                                           tatGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGa                              >  2:2731231/1‑93 (MQ=255)
                                                             tGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGAtc                            >  1:2764663/1‑93 (MQ=255)
                                                              gCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTTGGGACGTTCTGGAtct                           >  1:2948929/1‑93 (MQ=255)
                                                                   gAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTAcc                      >  2:828103/1‑93 (MQ=255)
                                                                   gAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTAcc                      <  1:3588713/93‑1 (MQ=255)
                                                                      ccATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGt                   <  2:1965960/93‑1 (MQ=255)
                                                                      ccATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGt                   >  1:1646890/1‑93 (MQ=255)
                                                                       cATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTGTGGATCTCTACCGGtt                  >  2:1364443/1‑93 (MQ=255)
                                                                         ttACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGttct                <  2:3095629/93‑1 (MQ=255)
                                                                           aCGTTCTACTTTCGCGCACAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGttcttc              <  2:1457132/93‑1 (MQ=255)
                                                                            cGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTcc             <  1:2143394/93‑1 (MQ=255)
                                                                              ttCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCTGGACGTTCTGGATCTCTACCGGTTCTTCCGc           >  2:2465635/1‑93 (MQ=255)
                                                                              ttCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGc           >  1:696593/1‑93 (MQ=255)
                                                                              ttCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGc           >  1:152900/1‑93 (MQ=255)
                                                                              ttCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGc           >  2:2343188/1‑93 (MQ=255)
                                                                                cTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCa         >  2:49930/1‑93 (MQ=255)
                                                                                 tACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCag        >  1:167881/1‑93 (MQ=255)
                                                                                 tACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCag        <  2:3522318/93‑1 (MQ=255)
                                                                                  aCTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCagt       >  2:3983400/1‑93 (MQ=255)
                                                                                    tttCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCagtag     >  1:3337222/1‑93 (MQ=255)
                                                                                    tttCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCagtag     <  1:3226657/93‑1 (MQ=255)
                                                                                    tttCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCagtag     <  2:3070130/93‑1 (MQ=255)
                                                                                     ttCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCGGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCagtagt    >  1:436430/1‑93 (MQ=255)
                                                                                      tCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTg   >  2:278666/1‑93 (MQ=255)
                                                                                      tCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTg   <  2:1082357/93‑1 (MQ=255)
                                                                                       cgcgCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGc  <  1:330450/93‑1 (MQ=255)
                                                                                       cgcgCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGc  <  2:57846/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGC  >  NC_000913/3333863‑3334042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCA  >  NC_000913/3333860‑3334046
                                                                                             |                                                                                             
AGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCG                                                                                               >  SRR4302535.2356528/1‑94 (MQ=60)
 GCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGC                                                                                              >  SRR4302535.2378671/1‑94 (MQ=60)
    CGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCA                                                                                           >  SRR4302535.783033/1‑94 (MQ=60)
     GCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAG                                                                                          <  SRR4302535.3610920/94‑1 (MQ=60)
      CATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGC                                                                                         >  SRR4302535.301930/1‑94 (MQ=60)
      CATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGC                                                                                         >  SRR4302535.3744403/1‑94 (MQ=60)
       ATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCT                                                                                        >  SRR4302535.1538023/1‑94 (MQ=60)
       ATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCT                                                                                        <  SRR4302535.1556627/94‑1 (MQ=60)
         GGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGG                                                                                      <  SRR4302535.1418524/94‑1 (MQ=60)
          GTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGC                                                                                     >  SRR4302535.860732/1‑94 (MQ=60)
           TTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCT                                                                                    <  SRR4302535.165877/94‑1 (MQ=60)
             TTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTA                                                                                  <  SRR4302535.2999711/94‑1 (MQ=60)
                ACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCA                                                                               <  SRR4302535.1595734/94‑1 (MQ=60)
                 CCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAG                                                                              >  SRR4302535.4348294/1‑94 (MQ=60)
                   AGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCT                                                                            >  SRR4302535.2981601/1‑94 (MQ=60)
                    GATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTT                                                                           >  SRR4302535.2623871/1‑94 (MQ=60)
                    GATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTT                                                                           <  SRR4302535.4325234/94‑1 (MQ=60)
                    GATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTT                                                                           >  SRR4302535.4717449/1‑94 (MQ=60)
                     ATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTC                                                                          >  SRR4302535.3248807/1‑94 (MQ=60)
                       CGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCAT                                                                        <  SRR4302535.1580312/94‑1 (MQ=60)
                       CGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCAT                                                                        >  SRR4302535.969411/1‑94 (MQ=60)
                        GTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATT                                                                       >  SRR4302535.4263508/1‑94 (MQ=60)
                        GTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATT                                                                       <  SRR4302535.854884/94‑1 (MQ=60)
                        GTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATT                                                                       >  SRR4302535.1154489/1‑94 (MQ=60)
                         TAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTG                                                                      <  SRR4302535.3927604/94‑1 (MQ=60)
                         TAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTG                                                                      <  SRR4302535.990668/94‑1 (MQ=60)
                          AAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGA                                                                     >  SRR4302535.1904720/1‑94 (MQ=60)
                          AAGGCGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGA                                                                     <  SRR4302535.948205/94‑1 (MQ=60)
                               GCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTAT                                                                <  SRR4302535.2071316/94‑1 (MQ=60)
                                CGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATC                                                               <  SRR4302535.2301541/94‑1 (MQ=60)
                                 GCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCG                                                              >  SRR4302535.1543779/1‑94 (MQ=60)
                                 GCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCG                                                              <  SRR4302535.469886/94‑1 (MQ=60)
                                  CAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGA                                                             >  SRR4302535.3336779/1‑94 (MQ=60)
                                   AGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGAC                                                            <  SRR4302535.1083427/94‑1 (MQ=60)
                                     AATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGT                                                          <  SRR4302535.4062256/94‑1 (MQ=60)
                                      ATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTC                                                         >  SRR4302535.3580490/1‑94 (MQ=60)
                                      ATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTC                                                         <  SRR4302535.3466715/94‑1 (MQ=60)
                                       TACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCT                                                        <  SRR4302535.3870165/94‑1 (MQ=60)
                                       TACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCT                                                        >  SRR4302535.3048479/1‑94 (MQ=60)
                                        ACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTT                                                       >  SRR4302535.1023486/1‑94 (MQ=60)
                                        ACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTT                                                       >  SRR4302535.3216777/1‑94 (MQ=60)
                                         CATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTT                                                      <  SRR4302535.3028007/94‑1 (MQ=60)
                                         CATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTT                                                      >  SRR4302535.483635/1‑94 (MQ=60)
                                          ATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTTTTTC                                                     >  SRR4302535.3747985/1‑94 (MQ=60)
                                          ATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTC                                                     >  SRR4302535.140570/1‑94 (MQ=60)
                                          ATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTC                                                     >  SRR4302535.835695/1‑94 (MQ=60)
                                          ATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTC                                                     <  SRR4302535.4600542/94‑1 (MQ=60)
                                          ATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTC                                                     <  SRR4302535.4142899/94‑1 (MQ=60)
                                          ATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTC                                                     >  SRR4302535.3763998/1‑94 (MQ=60)
                                             AACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGT                                                  <  SRR4302535.2646425/94‑1 (MQ=60)
                                              ACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTT                                                 <  SRR4302535.2941527/94‑1 (MQ=60)
                                               CGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTT                                                <  SRR4302535.22002/94‑1 (MQ=60)
                                                CTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTC                                               <  SRR4302535.3776397/94‑1 (MQ=60)
                                                 TCGCGGGCATCAATATTCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCG                                              <  SRR4302535.2117227/94‑1 (MQ=60)
                                                 TCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCG                                              >  SRR4302535.685116/1‑94 (MQ=60)
                                                    CGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGA                                           >  SRR4302535.94364/1‑94 (MQ=60)
                                                     GGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGAC                                          >  SRR4302535.1993298/1‑94 (MQ=60)
                                                      GGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACG                                         >  SRR4302535.4630954/1‑94 (MQ=60)
                                                       GCATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGT                                        >  SRR4302535.1944532/1‑94 (MQ=60)
                                                        CATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTT                                       <  SRR4302535.3066135/94‑1 (MQ=60)
                                                        CATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTT                                       >  SRR4302535.2104399/1‑94 (MQ=60)
                                                        CATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTT                                       <  SRR4302535.3080542/94‑1 (MQ=60)
                                                         ATCAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTC                                      >  SRR4302535.2044214/1‑94 (MQ=60)
                                                           CAATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTG                                    <  SRR4302535.3050337/94‑1 (MQ=60)
                                                            AATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGG                                   >  SRR4302535.3179577/1‑94 (MQ=60)
                                                            AATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGG                                   >  SRR4302535.1645929/1‑94 (MQ=60)
                                                            AATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGG                                   <  SRR4302535.649698/94‑1 (MQ=60)
                                                             ATATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGA                                  >  SRR4302535.1380078/1‑94 (MQ=60)
                                                               ATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATC                                >  SRR4302535.688720/1‑94 (MQ=60)
                                                               ATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATC                                >  SRR4302535.2784355/1‑94 (MQ=60)
                                                               ATGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATC                                >  SRR4302535.3186084/1‑94 (MQ=60)
                                                                TGCACAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTTGGGACGTTCTGGATCT                               >  SRR4302535.2969647/1‑94 (MQ=60)
                                                                    CAGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTGTCGGGACGTTCTGGATCTCTAC                           <  SRR4302535.831494/94‑1 (MQ=60)
                                                                     AGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACC                          >  SRR4302535.2138040/1‑94 (MQ=60)
                                                                     AGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACC                          >  SRR4302535.1554803/1‑94 (MQ=60)
                                                                     AGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACC                          >  SRR4302535.1869604/1‑94 (MQ=60)
                                                                     AGAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACC                          >  SRR4302535.3268086/1‑94 (MQ=60)
                                                                      GAACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCG                         <  SRR4302535.3613607/94‑1 (MQ=60)
                                                                        ACCATTACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGT                       >  SRR4302535.1658796/1‑94 (MQ=60)
                                                                             TACGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTC                  >  SRR4302535.3567107/1‑94 (MQ=60)
                                                                               CGTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCG                <  SRR4302535.2158812/94‑1 (MQ=60)
                                                                                GTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGC               >  SRR4302535.3393097/1‑94 (MQ=60)
                                                                                GTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGC               >  SRR4302535.701478/1‑94 (MQ=60)
                                                                                GTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGC               >  SRR4302535.153972/1‑94 (MQ=60)
                                                                                GTTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGC               >  SRR4302535.4009122/1‑94 (MQ=60)
                                                                                 TTCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGCTCTTCCGCC              >  SRR4302535.900014/1‑94 (MQ=60)
                                                                                  TCTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCA             <  SRR4302535.4664271/94‑1 (MQ=60)
                                                                                   CTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAG            >  SRR4302535.169045/1‑94 (MQ=60)
                                                                                   CTACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAG            >  SRR4302535.4207726/1‑94 (MQ=60)
                                                                                    TACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGT           <  SRR4302535.4313344/94‑1 (MQ=60)
                                                                                     ACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTA          <  SRR4302535.1195624/94‑1 (MQ=60)
                                                                                     ACTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCT‑GATCTCTACCGGTTCTTCCGCCAGGAG         >  SRR4302535.3171735/1‑94 (MQ=60)
                                                                                      CTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAG         <  SRR4302535.2523557/94‑1 (MQ=60)
                                                                                      CTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAG         <  SRR4302535.761141/94‑1 (MQ=60)
                                                                                      CTTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAG         >  SRR4302535.3360464/1‑94 (MQ=60)
                                                                                       TTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCGGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGT        >  SRR4302535.439473/1‑94 (MQ=60)
                                                                                       TTTCGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGT        <  SRR4302535.3249023/94‑1 (MQ=60)
                                                                                          CGCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCG     <  SRR4302535.332739/94‑1 (MQ=60)
                                                                                           GCGCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGG    <  SRR4302535.4549848/94‑1 (MQ=60)
                                                                                             GCCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCA  <  SRR4302535.1709894/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCA  >  NC_000913/3333860‑3334046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑