Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,403,483 A→G A137A (GCA→GCG)  accB → acetyl‑CoA carboxylase BCCP subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,403,4830AG99.5% 585.4 / ‑6.5 189A137A (GCA→GCG) accBacetyl‑CoA carboxylase BCCP subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (106/82);  total (106/83)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 220 total aligned reads displayed.

GAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTA  >  NC_000913/3403393‑3403572
                                                                                          |                                                                                         
  aaGTCAACGTGGGCGATACCCTGTGCATCGTTGAATCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTc                                                                                       <  1:4366365/93‑1 (MQ=255)
  aaGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTc                                                                                       >  1:3978561/1‑93 (MQ=255)
  aaGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTc                                                                                       <  2:3685734/93‑1 (MQ=255)
  aaGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTc                                                                                       <  1:778621/93‑1 (MQ=255)
   aGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCt                                                                                      <  1:2908352/93‑1 (MQ=255)
    gTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCCTGAAAGCGATTCTg                                                                                     >  1:204430/1‑93 (MQ=255)
    atCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTg                                                                                     <  1:2939840/92‑1 (MQ=255)
     tCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTgg                                                                                    <  1:2235447/93‑1 (MQ=255)
     tCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTgg                                                                                    <  1:4187811/93‑1 (MQ=255)
     tCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTgg                                                                                    >  2:2935783/1‑93 (MQ=255)
      cAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGt                                                                                   <  2:2463397/93‑1 (MQ=255)
       aaCGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTc                                                                                  >  1:4224538/1‑93 (MQ=255)
       aaCGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGCGAAAGCGATTCTGGTc                                                                                  >  2:836701/1‑93 (MQ=255)
          gTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGaa                                                                               <  2:2034140/93‑1 (MQ=255)
             ggCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGt                                                                            <  2:1585494/93‑1 (MQ=255)
              gCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTg                                                                           <  1:61425/93‑1 (MQ=255)
                gATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGTCAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGa                                                                         >  2:4274566/1‑93 (MQ=255)
                gATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGa                                                                         <  2:861726/93‑1 (MQ=255)
                 aTACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGAc                                                                        >  1:3653438/1‑93 (MQ=255)
                  tACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACa                                                                       >  2:3969504/1‑93 (MQ=255)
                   aCCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACaa                                                                      >  1:4217897/1‑93 (MQ=255)
                   aCCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACaa                                                                      >  2:464337/1‑93 (MQ=255)
                     ccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAAcc                                                                    <  2:2684073/93‑1 (MQ=255)
                     ccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAAcc                                                                    >  1:3486659/1‑93 (MQ=255)
                      cTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCg                                                                   <  2:4218091/93‑1 (MQ=255)
                      cTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCg                                                                   >  1:3223428/1‑93 (MQ=255)
                       tgtgCATCGTTGAAGCCATGAAAATGATGAATCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGCTTCTGGTCGAAAGTGGACAACCgg                                                                  >  2:1872929/1‑93 (MQ=255)
                       tgtgCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCgg                                                                  >  1:4549867/1‑93 (MQ=255)
                         tgCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTa                                                                <  2:3620548/93‑1 (MQ=255)
                         tgCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTa                                                                <  2:2424248/93‑1 (MQ=255)
                         tgCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTa                                                                <  2:3540714/93‑1 (MQ=255)
                         tgCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTa                                                                >  2:4423414/1‑93 (MQ=255)
                          gCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAg                                                               >  2:1771703/1‑93 (MQ=255)
                          gCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGGGAAATCGCTTCTGGTCGAAAGTGGCAAACCggaca                                                               >  1:2643622/1‑90 (MQ=255)
                            aTCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGaa                                                             >  1:1545266/1‑93 (MQ=255)
                             tCGTTGAAGCGATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAAt                                                            >  2:1521002/1‑93 (MQ=255)
                             tCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAAt                                                            >  2:674281/1‑93 (MQ=255)
                             tCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAAt                                                            >  2:4102770/1‑93 (MQ=255)
                             tCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAAt                                                            >  1:1910459/1‑93 (MQ=255)
                               gTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAAttt                                                          >  2:172218/1‑93 (MQ=255)
                               gTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAAttt                                                          >  1:1224996/1‑93 (MQ=255)
                               gTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAAttt                                                          >  1:47412/1‑93 (MQ=255)
                                ttGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTg                                                         >  1:2132070/1‑93 (MQ=255)
                                 tGAAGCCATGAAAATGATGAACAAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGa                                                        <  1:4486825/93‑1 (MQ=255)
                                   aaGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACg                                                      >  1:2528955/1‑93 (MQ=255)
                                     gCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAg                                                    >  2:1877952/1‑93 (MQ=255)
                                          gAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGcg                                               >  2:3817776/1‑92 (MQ=255)
                                           aaaaTGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTg                                              >  2:181552/1‑93 (MQ=255)
                                            aaaTGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTgg                                             >  2:4233909/1‑93 (MQ=255)
                                             aaTGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgt                                            <  1:1339213/93‑1 (MQ=255)
                                             aaTGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgt                                            >  1:233332/1‑93 (MQ=255)
                                              atgatgAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtc                                           >  2:3522116/1‑93 (MQ=255)
                                               tgatgaACCAGATCGAAGCGGACAAATCCGGTCCCGGGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtcg                                          <  2:2972964/93‑1 (MQ=255)
                                               agatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtcg                                          <  1:1658521/92‑1 (MQ=255)
                                                gatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtcgt                                         >  2:3466553/1‑93 (MQ=255)
                                                gatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtcgt                                         >  2:2886304/1‑93 (MQ=255)
                                                gatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtcgt                                         >  2:3557021/1‑93 (MQ=255)
                                                  tgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtcgtct                                       >  1:270167/1‑92 (MQ=255)
                                                  tgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCa                                       <  1:1990616/93‑1 (MQ=255)
                                                  tgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCa                                       <  2:3650183/93‑1 (MQ=255)
                                                     aCCAGATCGAAGCGGACAAACCGGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                    <  1:2487159/93‑1 (MQ=255)
                                                     aCCACATGGAAGCGGACAAATCCGGTCCCGTGAAAGCGATTCTGGTCGAAAGTGGACACCCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                    <  2:3402797/93‑1 (MQ=255)
                                                        aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGt                                 <  2:4529865/93‑1 (MQ=255)
                                                        aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGt                                 >  1:3141675/1‑93 (MQ=255)
                                                        aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGt                                 >  1:3431372/1‑93 (MQ=255)
                                                        aGATCGAAGCGCAAAAATCCGGTACCGGGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGt                                 <  2:3093492/93‑1 (MQ=255)
                                                         gATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTa                                >  1:3062843/1‑93 (MQ=255)
                                                         gATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTa                                >  1:71959/1‑93 (MQ=255)
                                                          aTCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTaa                               <  2:956708/93‑1 (MQ=255)
                                                          aTCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTaa                               >  2:1984729/1‑93 (MQ=255)
                                                           tCGAAGCGGACAACTCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAAc                              <  2:370927/93‑1 (MQ=255)
                                                           tCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAAc                              <  1:708296/93‑1 (MQ=255)
                                                           acggaGCGGACAAACCCGGTACCGTAAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAAc                              <  1:966218/89‑1 (MQ=255)
                                                             gagacGGACAAATAGCGTACTGTCAAAGATATTCTGGTGAAAAGGGAACACCCGGTAGAATTTGTTGAGCCTCTGGTCTTCATCGAGTAACGa                            <  2:937554/89‑1 (MQ=255)
                                                             gAAGCGGACAAGAGCGATACCGTGAAGGCGATTCTGGGCGAAAGTGGAAACCCGATAGAATTTGGCGAGCCGCTGGTCGTCATCGAGTAACGa                            <  1:831636/93‑1 (MQ=255)
                                                             gAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGa                            >  2:3751672/1‑93 (MQ=255)
                                                             gAAGCGCACAAGTCCGGAACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGa                            <  2:1995247/93‑1 (MQ=255)
                                                                gtggACAAATCGGGTACCGTGAAAGCGATTCGGGTCGAAAGTGGACAACCGGTAAAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGc                         <  1:1048158/91‑1 (MQ=255)
                                                                  ggACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGa                       >  2:3223623/1‑93 (MQ=255)
                                                                   gACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGac                      >  1:1441755/1‑92 (MQ=255)
                                                                     cAAATCGGGTACCGTGAAAGCGATTCTGGCCGAAGGTCGACAACGGGAAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACa                    <  1:2731883/93‑1 (MQ=255)
                                                                     cAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACa                    <  1:49830/93‑1 (MQ=255)
                                                                      aaaTCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACAt                   <  2:1325478/93‑1 (MQ=255)
                                                                        aTCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGc                 >  2:584720/1‑93 (MQ=255)
                                                                        aTCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGc                 >  1:1085729/1‑93 (MQ=255)
                                                                        aTCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGAACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGc                 <  1:4279723/93‑1 (MQ=255)
                                                                          ccGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTg               <  1:690713/93‑1 (MQ=255)
                                                                             gTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGGCGTCATCGAGTAACGAGGCGAACATGCTgggt            >  2:3283280/1‑91 (MQ=255)
                                                                               tccGGGAAAGGGATTTTGGTGAAAAGTGGGAAACCGGTAAAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATaa          <  2:4482995/92‑1 (MQ=255)
                                                                                ccGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATaaa         >  1:1617973/1‑93 (MQ=255)
                                                                                 cGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATaaaa        <  2:2259979/93‑1 (MQ=255)
                                                                                 cGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATaaaa        <  2:2184205/93‑1 (MQ=255)
                                                                                  gaaaaaGCGTTTTTGGTGAAAAGT‑GACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAAtt      <  2:3079809/90‑1 (MQ=255)
                                                                                   tGAAAGCGATTCTGGTCGAAGGTGGACACCCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAAtt      <  2:1294399/93‑1 (MQ=255)
                                                                                    gAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTg     >  1:374930/1‑93 (MQ=255)
                                                                                    gAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTg     >  2:1667504/1‑93 (MQ=255)
                                                                                      aaGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGtt   >  1:974721/1‑93 (MQ=255)
                                                                                      aaGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGtt   >  1:4098271/1‑93 (MQ=255)
                                                                                       aGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTa  <  1:185958/93‑1 (MQ=255)
                                                                                       aGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTa  <  2:428444/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTA  >  NC_000913/3403393‑3403572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGC  >  NC_000913/3403391‑3403576
                                                                                            |                                                                                             
CAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGA                                                                                              <  SRR4302535.1333942/94‑1 (MQ=60)
 AGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGAT                                                                                             >  SRR4302535.81499/1‑94 (MQ=60)
   AAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTC                                                                                           >  SRR4302535.4005748/1‑94 (MQ=60)
    AAGTCAACGTGGGCGATGCCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCT                                                                                          <  SRR4302535.2484837/94‑1 (MQ=60)
    AAGTCAACGTGGGCGATACCCTGTGCATCGTTGAATCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCT                                                                                          <  SRR4302535.4396243/94‑1 (MQ=60)
    AAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCT                                                                                          <  SRR4302535.784091/94‑1 (MQ=60)
     AGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTG                                                                                         <  SRR4302535.2928908/94‑1 (MQ=60)
     AGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCCTGAAAGCGATTCTG                                                                                         >  SRR4302535.205830/1‑94 (MQ=60)
      ATCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGG                                                                                        <  SRR4302535.2960515/94‑1 (MQ=60)
       TCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGT                                                                                       <  SRR4302535.4216474/94‑1 (MQ=60)
       TCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGT                                                                                       <  SRR4302535.2251349/94‑1 (MQ=60)
        CAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTC                                                                                      >  SRR4302535.4253443/1‑94 (MQ=60)
        CAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTC                                                                                      >  SRR4302535.2069394/1‑94 (MQ=60)
              GGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGT                                                                                >  SRR4302535.276047/1‑94 (MQ=60)
              GGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGT                                                                                <  SRR4302535.2741874/94‑1 (MQ=60)
               GGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTG                                                                               <  SRR4302535.975877/94‑1 (MQ=60)
                GCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGG                                                                              <  SRR4302535.61885/94‑1 (MQ=60)
                 CGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAATCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGA                                                                             <  SRR4302535.72481/94‑1 (MQ=60)
                 CGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGA                                                                             <  SRR4302535.3297002/94‑1 (MQ=60)
                  GATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGAC                                                                            >  SRR4302535.197511/1‑94 (MQ=60)
                  GATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGAC                                                                            >  SRR4302535.3678733/1‑94 (MQ=60)
                    TACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGACAGTGGACAA                                                                          >  SRR4302535.1977740/1‑94 (MQ=60)
                    TACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAA                                                                          >  SRR4302535.4246762/1‑94 (MQ=60)
                     TCCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAAC                                                                         <  SRR4302535.511416/94‑1 (MQ=60)
                     ACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAAC                                                                         <  SRR4302535.4701155/94‑1 (MQ=60)
                     ACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAAC                                                                         <  SRR4302535.1465016/94‑1 (MQ=60)
                      CCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACC                                                                        >  SRR4302535.3510865/1‑94 (MQ=60)
                      CCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACC                                                                        <  SRR4302535.4368959/94‑1 (MQ=60)
                       CCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCG                                                                       >  SRR4302535.3245772/1‑94 (MQ=60)
                       CCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCG                                                                       >  SRR4302535.1554807/1‑94 (MQ=60)
                        CTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGG                                                                      >  SRR4302535.3183608/1‑94 (MQ=60)
                        CTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGG                                                                      >  SRR4302535.309412/1‑94 (MQ=60)
                        CTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGG                                                                      >  SRR4302535.4580969/1‑94 (MQ=60)
                          GTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGGAAGCGATTCTGGTCGAAAGTGGTCAACCGGTA                                                                    >  SRR4302535.1882193/1‑94 (MQ=60)
                           TGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAG                                                                   <  SRR4302535.731229/94‑1 (MQ=60)
                           TGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGGGAAATCGCTTCTGGTCGAAAGTGGcaaaccggaca                                                                   >  SRR4302535.2662556/1‑83 (MQ=60)
                            GCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGA                                                                  <  SRR4302535.3547764/94‑1 (MQ=60)
                             AATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAA                                                                 >  SRR4302535.1556393/1‑94 (MQ=60)
                              ATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAAT                                                                >  SRR4302535.1924227/1‑94 (MQ=60)
                                CGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTT                                                              >  SRR4302535.47768/1‑94 (MQ=60)
                                CGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTT                                                              >  SRR4302535.703051/1‑94 (MQ=60)
                                CGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTT                                                              >  SRR4302535.1233756/1‑94 (MQ=60)
                                 GTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTG                                                             >  SRR4302535.2147440/1‑94 (MQ=60)
                                  TTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGA                                                            >  SRR4302535.2282973/1‑94 (MQ=60)
                                   TGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGAC                                                           >  SRR4302535.1630984/1‑94 (MQ=60)
                                   TGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGAC                                                           <  SRR4302535.1117702/94‑1 (MQ=60)
                                   TGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGAC                                                           <  SRR4302535.684499/94‑1 (MQ=60)
                                   TGAAGCCATGAAAATGATGAACAAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGAC                                                           <  SRR4302535.4517625/94‑1 (MQ=60)
                                    GAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACG                                                          <  SRR4302535.4528737/94‑1 (MQ=60)
                                    GAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACG                                                          >  SRR4302535.2547176/1‑94 (MQ=60)
                                      AGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAG                                                        <  SRR4302535.2939747/94‑1 (MQ=60)
                                         CATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCG                                                     >  SRR4302535.1072954/1‑94 (MQ=60)
                                         CATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCG                                                     >  SRR4302535.485014/1‑94 (MQ=60)
                                         CATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCG                                                     >  SRR4302535.1346988/1‑94 (MQ=60)
                                             AAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGG                                                 >  SRR4302535.442525/1‑94 (MQ=60)
                                              AAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGT                                                >  SRR4302535.234944/1‑94 (MQ=60)
                                               AATGATGAGCCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTC                                               <  SRR4302535.1348769/94‑1 (MQ=60)
                                               AATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTC                                               <  SRR4302535.1348768/94‑1 (MQ=60)
                                                aggatgaacaagctgGAAGCGGACAAATCGGGTACGGTGAAAGCGATGCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCG                                              <  SRR4302535.4250852/79‑1 (MQ=60)
                                                 TGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGT                                             >  SRR4302535.424527/1‑94 (MQ=60)
                                                 AGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGT                                             <  SRR4302535.1670509/94‑1 (MQ=60)
                                                  GATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTC                                            >  SRR4302535.2217877/1‑94 (MQ=60)
                                                   ATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCT                                           >  SRR4302535.272046/1‑94 (MQ=60)
                                                    TGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCAT                                          <  SRR4302535.2004950/94‑1 (MQ=60)
                                                       ACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGA                                       <  SRR4302535.3066643/94‑1 (MQ=60)
                                                       ACCAGATCGAAGCGGACAAACCGGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGA                                       <  SRR4302535.2505094/94‑1 (MQ=60)
                                                        CCGGATCGAAGCGGAAAAATCCGGTACCGTGAAAGCGATCCTGGTCAAAAGTGGACAACCGGTAGATTTGGACGAGCCGCTGGTCGTCATCGAG                                      <  SRR4302535.1860318/94‑1 (MQ=60)
                                                        CCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAG                                      <  SRR4302535.2702887/94‑1 (MQ=60)
                                                         CAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGT                                     >  SRR4302535.3455220/1‑94 (MQ=60)
                                                         CAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGT                                     >  SRR4302535.3163535/1‑94 (MQ=60)
                                                          AGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTA                                    >  SRR4302535.72484/1‑94 (MQ=60)
                                                          AGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTA                                    >  SRR4302535.3084256/1‑94 (MQ=60)
                                                          AGATCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTA                                    <  SRR4302535.3698701/94‑1 (MQ=60)
                                                             acggAGCGGACAAACCCGGTACCGTAAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACG                                 <  SRR4302535.973083/90‑1 (MQ=60)
                                                             TCGAAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACG                                 <  SRR4302535.713267/94‑1 (MQ=60)
                                                             TCGAAGCGAACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACG                                 <  SRR4302535.54216/94‑1 (MQ=60)
                                                               GAAGCGGACAAGAGCGATACCGTGAAGGCGATTCTGGGCGAAAGTGGAAACCCGATAGAATTTGGCGAGCCGCTGGTCGTCATCGAGTAACGAG                               <  SRR4302535.837464/94‑1 (MQ=38)
                                                                AAGCGGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGG                              <  SRR4302535.4378528/94‑1 (MQ=60)
                                                                  GTGGACAAATCGGGTACCGTGAAAGCGATTCGGGTCGAAAGTGGACAACCGGTAAAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCG                            <  SRR4302535.1055592/94‑1 (MQ=60)
                                                                    GGACAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAC                          >  SRR4302535.1452068/1‑94 (MQ=60)
                                                                       gtttcCCGGTACGGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACAT                       <  SRR4302535.2007609/89‑1 (MQ=60)
                                                                       CAAATCGGGTACCGTGAAAGCGATTCTGGCCGAAGGTCGACAACGGGAAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACAT                       <  SRR4302535.2751386/94‑1 (MQ=60)
                                                                       CAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACAT                       <  SRR4302535.4033634/94‑1 (MQ=60)
                                                                       CAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACAT                       <  SRR4302535.50200/94‑1 (MQ=60)
                                                                       CAAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACAT                       >  SRR4302535.421405/1‑94 (MQ=60)
                                                                        AAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATG                      >  SRR4302535.3396559/1‑94 (MQ=60)
                                                                        AAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATG                      >  SRR4302535.957911/1‑94 (MQ=60)
                                                                        AAATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATG                      >  SRR4302535.4279740/1‑94 (MQ=60)
                                                                         AATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGC                     >  SRR4302535.1093445/1‑94 (MQ=60)
                                                                          ATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCT                    <  SRR4302535.2576711/94‑1 (MQ=60)
                                                                          ATCCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGAACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCT                    <  SRR4302535.4309008/94‑1 (MQ=60)
                                                                            CCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGG                  <  SRR4302535.814208/94‑1 (MQ=60)
                                                                            CCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGG                  <  SRR4302535.695553/94‑1 (MQ=60)
                                                                            CCGGTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGG                  >  SRR4302535.2768436/1‑94 (MQ=60)
                                                                              GGTACCGTGAAAGCGATTCTGGCCGAAAGTGGACATCCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGAT                <  SRR4302535.3811397/94‑1 (MQ=60)
                                                                               GTACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATA               <  SRR4302535.3508340/94‑1 (MQ=60)
                                                                                 ACCGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAA             >  SRR4302535.1629626/1‑94 (MQ=60)
                                                                                   CGTGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAAT           >  SRR4302535.1629095/1‑94 (MQ=60)
                                                                                    GTGAAAGAAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATT          <  SRR4302535.2440465/94‑1 (MQ=60)
                                                                                    GGGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATT          <  SRR4302535.1823123/94‑1 (MQ=60)
                                                                                     TGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTG         >  SRR4302535.377524/1‑94 (MQ=60)
                                                                                     GGAAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTG         <  SRR4302535.4102229/94‑1 (MQ=60)
                                                                                       AAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTT       >  SRR4302535.4126326/1‑94 (MQ=60)
                                                                                       AAAGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTT       >  SRR4302535.981641/1‑94 (MQ=60)
                                                                                         AGCGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTAT     <  SRR4302535.187241/94‑1 (MQ=60)
                                                                                           CGATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTG   >  SRR4302535.4122479/1‑94 (MQ=60)
                                                                                            GATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGC  >  SRR4302535.30526/1‑94 (MQ=60)
                                                                                            |                                                                                             
CAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGC  >  NC_000913/3403391‑3403576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑