Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,403,945 T→C V131A (GTA→GCA)  accC → acetyl CoA carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,403,9450TC99.6% 694.5 / ‑5.0 229V131A (GTA→GCA) accCacetyl CoA carboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (107/121);  total (107/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01

Only 100 of 246 total aligned reads displayed.

TCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403855‑3404034
                                                                                          |                                                                                         
tCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAc                                                                                         >  2:1308219/1‑93 (MQ=255)
tCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAc                                                                                         >  2:3804656/1‑93 (MQ=255)
  ttCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCg                                                                                       >  1:4226373/1‑93 (MQ=255)
  ttCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCg                                                                                       >  1:220786/1‑93 (MQ=255)
   tCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCgg                                                                                      <  1:1025809/93‑1 (MQ=255)
   tCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCgg                                                                                      >  2:1620412/1‑93 (MQ=255)
     aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    >  1:3845198/1‑93 (MQ=255)
     aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    <  2:720748/93‑1 (MQ=255)
     aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    <  2:623871/93‑1 (MQ=255)
      ttGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGtt                                                                                   >  1:2449409/1‑93 (MQ=255)
      ttGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGtt                                                                                   >  1:2362739/1‑93 (MQ=255)
        ggCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCt                                                                                 <  1:3655145/93‑1 (MQ=255)
          cccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGa                                                                               <  1:4105196/93‑1 (MQ=255)
           ccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAc                                                                              <  1:3744526/93‑1 (MQ=255)
            cGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACg                                                                             >  1:1950189/1‑93 (MQ=255)
            cGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACg                                                                             >  2:3377712/1‑93 (MQ=255)
             gAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACgg                                                                            >  1:3954336/1‑93 (MQ=255)
               aaGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGcc                                                                          >  1:2303995/1‑93 (MQ=255)
               aaGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGcc                                                                          >  1:716455/1‑93 (MQ=255)
               aaGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGcc                                                                          >  1:4553535/1‑93 (MQ=255)
               aaGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGcc                                                                          <  1:1387050/93‑1 (MQ=255)
                 gCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCg                                                                        >  2:1416582/1‑93 (MQ=255)
                 gCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCg                                                                        >  1:840580/1‑93 (MQ=255)
                  cAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGc                                                                       <  2:3770159/93‑1 (MQ=255)
                    gAAACCATTCGCCTGATGGGCGACAAAGTATGCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTg                                                                     >  2:786301/1‑93 (MQ=255)
                     aaaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTgg                                                                    >  1:1471466/1‑93 (MQ=255)
                     aaaCCATTCGCCTGATGGGCGACAAAGTATCCGCAAGCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTgg                                                                    <  2:2037636/93‑1 (MQ=255)
                      aaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTggg                                                                   <  2:2330493/93‑1 (MQ=255)
                      aaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTggg                                                                   <  2:293298/93‑1 (MQ=255)
                      aaCCATTCACCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTggg                                                                   >  2:4571878/1‑93 (MQ=255)
                       aCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGc                                                                  >  2:3275407/1‑93 (MQ=255)
                        ccATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcg                                                                 <  2:106389/93‑1 (MQ=255)
                         cATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcga                                                                <  1:4278316/93‑1 (MQ=255)
                          aTTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgac                                                               <  2:4146811/93‑1 (MQ=255)
                           ttCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgacg                                                              >  2:86708/1‑93 (MQ=255)
                             cGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGat                                                            <  2:2685312/93‑1 (MQ=255)
                              gCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGata                                                           <  1:75202/93‑1 (MQ=255)
                              gCCTGATGGGCGACAAAGTATCCGCAAGCGCGGCGATGAAAAAAGCGGGCGTCCCTGGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGata                                                           <  2:3673170/93‑1 (MQ=255)
                               ccTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGatat                                                          <  2:2572054/93‑1 (MQ=255)
                               ccTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGatat                                                          <  2:1705195/93‑1 (MQ=255)
                                cTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         >  1:790866/1‑93 (MQ=255)
                                cTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         <  1:3246199/93‑1 (MQ=255)
                                cTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACAATATg                                                         <  2:574574/93‑1 (MQ=255)
                                 tGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATgg                                                        >  2:1997155/1‑93 (MQ=255)
                                  gATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGa                                                       <  1:1787683/93‑1 (MQ=255)
                                  gATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGa                                                       <  2:1026268/93‑1 (MQ=255)
                                  gATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGa                                                       <  1:2012036/93‑1 (MQ=255)
                                  gATGGGCGACAAAGTAGCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGa                                                       <  1:4122454/93‑1 (MQ=255)
                                    tGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATa                                                     >  1:4668437/1‑93 (MQ=255)
                                    tGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATa                                                     <  1:3186727/93‑1 (MQ=255)
                                     gggCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaa                                                    <  2:1173315/93‑1 (MQ=255)
                                     gggCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaa                                                    >  2:3930839/1‑93 (MQ=255)
                                      ggCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaa                                                   <  2:2308799/93‑1 (MQ=255)
                                       gCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaaa                                                  <  2:3636381/93‑1 (MQ=255)
                                        cGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaaaa                                                 <  1:1127075/93‑1 (MQ=255)
                                        cGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaaaa                                                 <  1:1137667/93‑1 (MQ=255)
                                         gACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCCTGCGCACCGGGGTCTGACCGCCCGCTGGGCGACGATATGGATAAAAAc                                                >  2:1502532/1‑93 (MQ=255)
                                          aCAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAcc                                               <  1:592853/93‑1 (MQ=255)
                                           cAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCg                                              <  2:2470675/93‑1 (MQ=255)
                                            aaaGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGt                                             <  2:1490071/93‑1 (MQ=255)
                                            aaaGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGt                                             >  2:4072873/1‑93 (MQ=255)
                                            aaaGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGt                                             >  2:2849133/1‑93 (MQ=255)
                                              aGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGACCCTTGCGCACCGGGGTCTGACGGCCCGCTCGGCGTCGATACGGACAAGAACCGTGc                                           >  2:2451040/1‑93 (MQ=255)
                                                  tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       <  2:421151/93‑1 (MQ=255)
                                                  tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       >  1:304391/1‑93 (MQ=255)
                                                   ccGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTg                                      >  2:970402/1‑93 (MQ=255)
                                                   ccGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTg                                      >  1:3612501/1‑93 (MQ=255)
                                                     gCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCt                                    >  1:2169609/1‑93 (MQ=255)
                                                       aaTCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTaa                                  >  1:444348/1‑93 (MQ=255)
                                                       aaTCGCGGCGATGAAAAAAGCGGGCGCCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTaa                                  <  2:1561983/93‑1 (MQ=255)
                                                        aTCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTaaa                                 <  1:407907/93‑1 (MQ=255)
                                                        aTCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTaaa                                 <  1:2198005/93‑1 (MQ=255)
                                                        aTCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTaaa                                 >  1:2592165/1‑93 (MQ=255)
                                                         tCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAAc                                <  1:1885201/93‑1 (MQ=255)
                                                         tCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAAc                                <  1:3860039/93‑1 (MQ=255)
                                                             ggcgATGAAAAAAGCGGGCGTCCCTCGCGCGCCGGGTTCTGACGCCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAt                            <  2:2884606/93‑1 (MQ=255)
                                                               cgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTg                          >  2:4012920/1‑93 (MQ=255)
                                                                 aTGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGt                        <  2:3891927/93‑1 (MQ=255)
                                                                   gAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGGCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTa                      <  2:3055569/93‑1 (MQ=255)
                                                                   gAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTa                      <  1:2726623/93‑1 (MQ=255)
                                                                   aaaaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTa                      <  2:1814956/92‑1 (MQ=255)
                                                                      aaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATcc                   <  1:706033/93‑1 (MQ=255)
                                                                       aaaTCGGGCGTCCCTTGCGCGCGGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCg                  <  1:3555139/93‑1 (MQ=255)
                                                                       aaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCg                  >  1:4350498/1‑93 (MQ=255)
                                                                          gCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTg               >  1:524176/1‑93 (MQ=255)
                                                                          gCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTg               >  2:436438/1‑93 (MQ=255)
                                                                            gggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAt             >  1:3568771/1‑93 (MQ=255)
                                                                             ggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAtt            >  2:453860/1‑93 (MQ=255)
                                                                             ggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAtt            <  2:2190953/93‑1 (MQ=255)
                                                                              gCGTCCCTTGCGCACCGGGTTCTGACGGCTCGGTGGGCGAAGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTa           <  1:3420298/93‑1 (MQ=255)
                                                                               cGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAt          <  1:4602356/93‑1 (MQ=255)
                                                                               cGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAt          >  1:2231443/1‑93 (MQ=255)
                                                                                gTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATc         <  2:2549927/93‑1 (MQ=255)
                                                                                 tCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCa        >  2:1965338/1‑93 (MQ=255)
                                                                                 tCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCa        >  2:2086566/1‑93 (MQ=255)
                                                                                 tCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCa        <  1:376712/93‑1 (MQ=255)
                                                                                  cccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaa       <  2:27618/93‑1 (MQ=255)
                                                                                    cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     <  1:3989070/93‑1 (MQ=255)
                                                                                    cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     <  2:3347446/93‑1 (MQ=255)
                                                                                     ttGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGc    <  1:287156/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403855‑3404034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCGG  >  NC_000913/3403854‑3404038
                                                                                           |                                                                                             
ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAC                                                                                             >  SRR4302535.1829488/1‑94 (MQ=60)
ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAC                                                                                             >  SRR4302535.1284011/1‑94 (MQ=60)
ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAC                                                                                             >  SRR4302535.4590600/1‑94 (MQ=60)
 TCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACC                                                                                            >  SRR4302535.4652351/1‑94 (MQ=60)
  CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                           >  SRR4302535.222308/1‑94 (MQ=60)
  CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                           >  SRR4302535.4255291/1‑94 (MQ=60)
  CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                           >  SRR4302535.3879669/1‑94 (MQ=60)
  CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                           >  SRR4302535.2375383/1‑94 (MQ=60)
  CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                           <  SRR4302535.2222325/94‑1 (MQ=60)
    TCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGG                                                                                         <  SRR4302535.1033095/94‑1 (MQ=60)
     CATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGT                                                                                        >  SRR4302535.3871675/1‑94 (MQ=60)
      ATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTT                                                                                       >  SRR4302535.2467059/1‑94 (MQ=60)
      ATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTT                                                                                       >  SRR4302535.2379709/1‑94 (MQ=60)
         GGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTG                                                                                    <  SRR4302535.3680446/94‑1 (MQ=60)
           CCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAC                                                                                  <  SRR4302535.4133302/94‑1 (MQ=60)
            CCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACG                                                                                 <  SRR4302535.607879/94‑1 (MQ=60)
            CCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACG                                                                                 <  SRR4302535.3770434/94‑1 (MQ=60)
            CCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACG                                                                                 >  SRR4302535.1964229/1‑94 (MQ=60)
             CGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGG                                                                                >  SRR4302535.3981332/1‑94 (MQ=60)
             CGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGC                                                                                >  SRR4302535.715630/1‑94 (MQ=60)
               AAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCC                                                                              >  SRR4302535.2320474/1‑94 (MQ=60)
               AAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCC                                                                              >  SRR4302535.721489/1‑94 (MQ=60)
               AAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCC                                                                              >  SRR4302535.4584646/1‑94 (MQ=60)
                AAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCC                                                                             <  SRR4302535.1396949/94‑1 (MQ=60)
                AAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGCTCTGACGGACC                                                                             >  SRR4302535.3658503/1‑94 (MQ=60)
                 AGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCG                                                                            >  SRR4302535.4142256/1‑94 (MQ=60)
                 AGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCG                                                                            >  SRR4302535.846482/1‑94 (MQ=60)
                   CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          <  SRR4302535.3311042/94‑1 (MQ=60)
                   CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                          <  SRR4302535.416304/94‑1 (MQ=60)
                    AGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTG                                                                         >  SRR4302535.958016/1‑94 (MQ=60)
                    AGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGCTCTGACGGCCCGCTG                                                                         >  SRR4302535.4285346/1‑94 (MQ=60)
                     GAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGG                                                                        <  SRR4302535.718115/94‑1 (MQ=60)
                     GAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGG                                                                        >  SRR4302535.1482003/1‑94 (MQ=60)
                      AAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGG                                                                       >  SRR4302535.2219678/1‑94 (MQ=60)
                       AACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGC                                                                      >  SRR4302535.36219/1‑94 (MQ=60)
                       AACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGC                                                                      <  SRR4302535.4445813/94‑1 (MQ=60)
                        ACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCG                                                                     <  SRR4302535.3236388/94‑1 (MQ=60)
                         CCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGA                                                                    <  SRR4302535.4028664/94‑1 (MQ=60)
                          CATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGAC                                                                   >  SRR4302535.529227/1‑94 (MQ=60)
                          CATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGAC                                                                   >  SRR4302535.577647/1‑94 (MQ=60)
                          CATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGAC                                                                   <  SRR4302535.1143276/94‑1 (MQ=60)
                          CATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGAC                                                                   <  SRR4302535.4307594/94‑1 (MQ=60)
                           ATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACG                                                                  <  SRR4302535.4138780/94‑1 (MQ=60)
                           ATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACG                                                                  <  SRR4302535.4273033/94‑1 (MQ=60)
                            TTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGA                                                                 >  SRR4302535.722864/1‑94 (MQ=60)
                              CGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATA                                                               <  SRR4302535.4512864/94‑1 (MQ=60)
                               GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              <  SRR4302535.465658/94‑1 (MQ=60)
                               GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              <  SRR4302535.75752/94‑1 (MQ=60)
                               GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              >  SRR4302535.206546/1‑94 (MQ=60)
                               GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                              <  SRR4302535.1789398/94‑1 (MQ=60)
                                CCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATG                                                             >  SRR4302535.796418/1‑94 (MQ=60)
                                CCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATG                                                             >  SRR4302535.1924388/1‑94 (MQ=60)
                                CCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATG                                                             >  SRR4302535.2761777/1‑94 (MQ=60)
                                 CTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                            <  SRR4302535.3268742/94‑1 (MQ=60)
                                 CTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                            <  SRR4302535.3592058/94‑1 (MQ=60)
                                  TGATGGGGGACAAAGTATCCGCAATCGGGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTCCTGACGGCCCGCTGGGCGACGATATGGA                                                           <  SRR4302535.3713385/94‑1 (MQ=60)
                                   GATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAT                                                          <  SRR4302535.2026531/94‑1 (MQ=60)
                                   GATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAT                                                          <  SRR4302535.1800615/94‑1 (MQ=60)
                                   GATGGGCGACAAAGTAGCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAT                                                          <  SRR4302535.4150663/94‑1 (MQ=60)
                                    ATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATA                                                         >  SRR4302535.4699980/1‑94 (MQ=60)
                                    ATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATA                                                         <  SRR4302535.1976034/94‑1 (MQ=60)
                                     TGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAA                                                        <  SRR4302535.3208765/94‑1 (MQ=60)
                                         CGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAC                                                    <  SRR4302535.1145780/94‑1 (MQ=60)
                                         CGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAC                                                    <  SRR4302535.1135124/94‑1 (MQ=60)
                                          GACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACC                                                   <  SRR4302535.3823330/94‑1 (MQ=60)
                                           ACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCG                                                  <  SRR4302535.597008/94‑1 (MQ=60)
                                           ACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCG                                                  <  SRR4302535.3828278/94‑1 (MQ=60)
                                             AAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTG                                                <  SRR4302535.3581531/94‑1 (MQ=60)
                                               AGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCC                                              >  SRR4302535.2364054/1‑94 (MQ=60)
                                                GTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTAGGCGACGATATGGATAAAAACCGAGCCA                                             >  SRR4302535.1514071/1‑94 (MQ=60)
                                                  ATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATT                                           <  SRR4302535.2094778/94‑1 (MQ=60)
                                                  ATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATT                                           >  SRR4302535.306503/1‑94 (MQ=60)
                                                  ATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATT                                           >  SRR4302535.2942287/1‑94 (MQ=60)
                                                   TCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTG                                          >  SRR4302535.3637551/1‑94 (MQ=60)
                                                    CCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGCGCGACGATATGGATAAAAACCGTGCCATTGC                                         >  SRR4302535.4031794/1‑94 (MQ=60)
                                                     CGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCT                                        >  SRR4302535.2185148/1‑94 (MQ=60)
                                                      GCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCAGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTA                                       >  SRR4302535.2302066/1‑94 (MQ=60)
                                                       CAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAA                                      >  SRR4302535.447457/1‑94 (MQ=60)
                                                        AATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAA                                     <  SRR4302535.1100677/94‑1 (MQ=60)
                                                        AATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAA                                     >  SRR4302535.2570956/1‑94 (MQ=60)
                                                        AATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAA                                     >  SRR4302535.2610785/1‑94 (MQ=60)
                                                         ATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAAC                                    <  SRR4302535.2213667/94‑1 (MQ=60)
                                                         ATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAAC                                    <  SRR4302535.410764/94‑1 (MQ=60)
                                                          TCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACG                                   <  SRR4302535.1898785/94‑1 (MQ=60)
                                                          TCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACG                                   <  SRR4302535.3886604/94‑1 (MQ=60)
                                                           CGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGC                                  >  SRR4302535.1958914/1‑94 (MQ=60)
                                                             CGGCGATGAAAAAAGCGGGCGTCCCTTGCGCTCCGAGTTCTGACGGCCCGCTGTGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAT                                <  SRR4302535.2408865/94‑1 (MQ=60)
                                                             CGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAT                                >  SRR4302535.3184523/1‑94 (MQ=60)
                                                              GGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATT                               <  SRR4302535.2526168/94‑1 (MQ=60)
                                                                CGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGG                             >  SRR4302535.1166336/1‑94 (MQ=60)
                                                                  ATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTT                           >  SRR4302535.2849716/1‑94 (MQ=60)
                                                                  ATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTT                           >  SRR4302535.790639/1‑94 (MQ=60)
                                                                  ATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTT                           <  SRR4302535.4126821/94‑1 (MQ=60)
                                                                    GAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTAT                         <  SRR4302535.776949/94‑1 (MQ=60)
                                                                    GAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTAT                         <  SRR4302535.2198488/94‑1 (MQ=60)
                                                                    GAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTAT                         <  SRR4302535.2746098/94‑1 (MQ=60)
                                                                      AAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCC                       <  SRR4302535.3982604/94‑1 (MQ=60)
                                                                      AAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCC                       <  SRR4302535.811066/94‑1 (MQ=60)
                                                                       AAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCG                      >  SRR4302535.4380261/1‑94 (MQ=60)
                                                                       AAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCG                      <  SRR4302535.3939759/94‑1 (MQ=60)
                                                                       AAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCG                      <  SRR4302535.710992/94‑1 (MQ=60)
                                                                        AAATCGGGCGTCCCTTGCGCGCGGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGG                     <  SRR4302535.3579800/94‑1 (MQ=60)
                                                                         AAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGT                    <  SRR4302535.3932541/94‑1 (MQ=60)
                                                                         AAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGT                    >  SRR4302535.4551927/1‑94 (MQ=60)
                                                                          AGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTG                   <  SRR4302535.397032/94‑1 (MQ=60)
                                                                          AGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTG                   >  SRR4302535.527838/1‑94 (MQ=60)
                                                                            CGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAT                 >  SRR4302535.3593518/1‑94 (MQ=60)
                                                                            CGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAT                 <  SRR4302535.1458719/94‑1 (MQ=60)
                                                                               GCGTCCCTTGCGCACCGGGTTCTGACGGCTCGGTGGGCGAAGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAT              <  SRR4302535.3444087/94‑1 (MQ=60)
                                                                               GCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAT              >  SRR4302535.2247310/1‑94 (MQ=60)
                                                                                CGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATC             <  SRR4302535.4633654/94‑1 (MQ=60)
                                                                                CGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATA             >  SRR4302535.3792804/1‑94 (MQ=60)
                                                                                  TCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAA           <  SRR4302535.379325/94‑1 (MQ=60)
                                                                                  TCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAA           >  SRR4302535.3743121/1‑94 (MQ=60)
                                                                                  TCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAA           <  SRR4302535.4344939/94‑1 (MQ=60)
                                                                                  TCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAA           >  SRR4302535.3083276/1‑94 (MQ=60)
                                                                                   CCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAA          >  SRR4302535.3481217/1‑94 (MQ=60)
                                                                                    CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG         <  SRR4302535.2675311/94‑1 (MQ=60)
                                                                                    CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG         >  SRR4302535.3357826/1‑94 (MQ=60)
                                                                                    CCTTGCGCACCGGGTTCTGACGACCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG         <  SRR4302535.4711457/94‑1 (MQ=60)
                                                                                     CTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGC        <  SRR4302535.4016353/94‑1 (MQ=60)
                                                                                      TTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCC       <  SRR4302535.289167/94‑1 (MQ=60)
                                                                                       TGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTCATCCGGTGATTATCAAAGCCT      <  SRR4302535.2239093/94‑1 (MQ=60)
                                                                                         CGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCC    >  SRR4302535.2144810/1‑94 (MQ=60)
                                                                                         CGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGACATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCC    <  SRR4302535.2538102/94‑1 (MQ=60)
                                                                                          GCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCG   <  SRR4302535.2963150/94‑1 (MQ=60)
                                                                                          GCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCG   <  SRR4302535.3443545/94‑1 (MQ=60)
                                                                                          GCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCG   <  SRR4302535.4405461/94‑1 (MQ=60)
                                                                                          GCACCGGGTGCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCG   <  SRR4302535.4153062/94‑1 (MQ=60)
                                                                                           CACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCGG  <  SRR4302535.624012/94‑1 (MQ=60)
                                                                                           CACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCGG  >  SRR4302535.4191881/1‑94 (MQ=60)
                                                                                           CACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCGG  <  SRR4302535.1901180/94‑1 (MQ=60)
                                                                                           CACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCGG  >  SRR4302535.499692/1‑94 (MQ=60)
                                                                                           |                                                                                             
ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCTCCGG  >  NC_000913/3403854‑3404038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑