Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,481,725 (C)6→7 coding (887/1023 nt) yheT → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,481,7191.C95.2% 749.9 / 28.1 249coding (881/1023 nt)yheThypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (5/7);  new base C (114/123);  total (119/130)
Fisher's exact test for biased strand distribution p-value = 7.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 276 total aligned reads displayed.

GATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCT  >  NC_000913/3481627‑3481806
                                                                                              |                                                                                        
gATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGtc‑t                                                                                           >  2:4336687/1‑93 (MQ=255)
 aTGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGtc‑t‑c                                                                                         >  2:2224312/1‑93 (MQ=255)
   tcTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGGTTATGGATCATCAGGTGATCCCGAAAGCGGAAAGTC‑T‑ccc                                                                                       >  1:637774/2‑93 (MQ=255)
    cTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑cccc                                                                                      <  2:2948390/93‑1 (MQ=255)
      gAACCGGATCGCCAAACCGACGCTGATTATTAACGCCAAAGACGATCCGTTTATGGGTCATCAGGTGATCCCGAAACCGGAAAGCC‑T‑ccccc‑c                                                                                   >  2:3018181/1‑93 (MQ=255)
       aaCCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑ccccc‑cc                                                                                  <  1:1026809/93‑2 (MQ=255)
       aaCCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑ccccc‑cc                                                                                  <  2:2818747/93‑2 (MQ=255)
          cGGATCGCCAACCCGACTCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑ccccc‑ccgca                                                                               <  2:4585654/93‑5 (MQ=255)
            gATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAgg                                                                              <  1:1606316/93‑1 (MQ=255)
            gATCCCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAgg                                                                              <  2:1314175/93‑1 (MQ=255)
            gATCACCANACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAgg                                                                              <  2:2895045/93‑1 (MQ=255)
                 ccAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAg                                                                         >  1:687314/1‑93 (MQ=255)
                 ccAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAg                                                                         <  1:4312140/93‑1 (MQ=255)
                  cAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAga                                                                        >  2:1343573/1‑92 (MQ=255)
                  cAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGt                                                                        <  2:3579626/93‑1 (MQ=255)
                   aaaCCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTa                                                                       >  1:1019155/1‑93 (MQ=255)
                    aaCCGACGCTGATTATTCACCCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGCAACCGGAACGTC‑TCCCCCC‑CGCAGGTGGAGCAt                                                                      >  1:1307744/1‑93 (MQ=255)
                      ccGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCa                                                                    >  2:1921700/1‑93 (MQ=255)
                      ccGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCa                                                                    >  2:3244297/1‑93 (MQ=255)
                      ccGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCa                                                                    <  1:2601826/93‑1 (MQ=255)
                         tcGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAact                                                                 >  1:4425295/2‑93 (MQ=255)
                          cGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCCGGTGGAGTATCAactg                                                                >  2:3414604/1‑93 (MQ=255)
                          cGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAactg                                                                <  2:792394/93‑1 (MQ=255)
                            cTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAactgac                                                              <  1:2700665/93‑1 (MQ=255)
                            cTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAactgac                                                              >  2:4262561/1‑93 (MQ=255)
                            cTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAactgac                                                              >  1:2375245/1‑93 (MQ=255)
                            cTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAactgac                                                              <  1:1498255/93‑1 (MQ=255)
                             tcattattCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCAC‑CCCCC‑CGCAGGTGGAGTATCAactgact                                                             <  1:4139492/91‑1 (MQ=255)
                             tGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCATGTGGAGTATCAactgact                                                             <  2:2348584/93‑1 (MQ=255)
                             tGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAactgact                                                             >  1:3378310/1‑93 (MQ=255)
                               attattCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAActgactga                                                           >  2:3311036/1‑93 (MQ=255)
                                ttattCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGaa                                                          >  1:4526942/1‑93 (MQ=255)
                                ttattCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGaa                                                          >  2:1764370/1‑93 (MQ=255)
                                 tattCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAAc                                                         >  1:1861814/1‑93 (MQ=255)
                                 tattCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAAc                                                         >  1:299696/1‑93 (MQ=255)
                                  attCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACa                                                        <  2:2563791/93‑1 (MQ=255)
                                   ttCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACAt                                                       >  2:2542033/1‑93 (MQ=255)
                                    tCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATg                                                      <  2:2061846/93‑1 (MQ=255)
                                    tCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATg                                                      >  1:3672287/1‑93 (MQ=255)
                                    tCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATg                                                      <  2:3837953/93‑1 (MQ=255)
                                     cACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATgg                                                     <  1:4365769/93‑1 (MQ=255)
                                     cACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATgg                                                     <  2:1703692/93‑1 (MQ=255)
                                     cACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATgg                                                     <  2:374975/93‑1 (MQ=255)
                                      aCGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGc                                                    <  2:1109915/93‑1 (MQ=255)
                                         ccAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGt                                                 <  1:56192/93‑1 (MQ=255)
                                          cAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTc                                                <  1:74448/93‑1 (MQ=255)
                                           aaaGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGCCTGAACATGGCGGTCa                                               >  2:907311/1‑93 (MQ=255)
                                           aaaGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGAATCAACTGACTGAACATGGCGGTCa                                               >  2:4412088/1‑93 (MQ=255)
                                           aaaGACGATCCGTTTATGGATCATCAGGTAATACCGAAACCGGAAAGTC‑TCCCCCC‑CGCTGGAGGAGAATCAACTGACTGAACATGGCGGTCa                                               >  2:2459515/1‑93 (MQ=255)
                                            aaGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCAt                                              >  1:873059/1‑93 (MQ=255)
                                              gtcGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGt                                            <  1:2184593/91‑1 (MQ=255)
                                              gACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGt                                            >  2:706200/1‑93 (MQ=255)
                                              gACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGt                                            <  1:1539861/93‑1 (MQ=255)
                                               aCGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGtt                                           <  2:4181847/93‑1 (MQ=255)
                                                cGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTg                                          <  2:449835/93‑1 (MQ=255)
                                                cGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTg                                          >  1:1398309/1‑93 (MQ=255)
                                                 gATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTgg                                         >  1:3229248/1‑93 (MQ=255)
                                                  aTCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGc                                        >  1:1057967/1‑93 (MQ=255)
                                                  aTCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGc                                        <  2:1777406/93‑1 (MQ=255)
                                                   tCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCt                                       <  2:3079179/93‑1 (MQ=255)
                                                   tCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCt                                       >  1:2060266/1‑93 (MQ=255)
                                                    ccGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCtt                                      <  1:654350/93‑1 (MQ=255)
                                                    ccGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCtt                                      <  1:2979251/93‑1 (MQ=255)
                                                    ccGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCtt                                      >  2:1194515/1‑93 (MQ=255)
                                                      gTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTa                                    >  2:4680632/1‑93 (MQ=255)
                                                         tATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTg                                 >  1:4215702/1‑93 (MQ=255)
                                                         tATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTg                                 <  2:2701453/93‑1 (MQ=255)
                                                         tATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTg                                 <  1:2268561/93‑1 (MQ=255)
                                                           tGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGc                               >  1:3288689/1‑93 (MQ=255)
                                                           tGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGc                               <  1:3594941/93‑1 (MQ=255)
                                                            ggATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATTAACTGACTGAACATTGCGGTCATGTTGGCTTTATTGGCg                              >  2:4300013/1‑93 (MQ=255)
                                                              atcatcAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGt                            >  1:2853452/1‑93 (MQ=255)
                                                              atcatcAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGt                            >  2:1555269/1‑93 (MQ=255)
                                                              atcatcAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCTCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGt                            >  2:859294/1‑93 (MQ=255)
                                                                catcaGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTAc                          >  1:4603236/1‑93 (MQ=255)
                                                                 atcaGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGGACa                         >  2:4034682/1‑93 (MQ=255)
                                                                  tcaGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACAt                        >  1:4679008/1‑93 (MQ=255)
                                                                  tcaGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACAt                        <  1:1331780/93‑1 (MQ=255)
                                                                    aGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTa                      <  2:2021913/93‑1 (MQ=255)
                                                                    aGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTa                      <  1:964430/93‑1 (MQ=255)
                                                                    aGGTGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTa                      <  2:2274508/93‑1 (MQ=255)
                                                                       tGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACtt                   >  1:170872/1‑93 (MQ=255)
                                                                       tGATCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACtt                   <  1:1428792/93‑1 (MQ=255)
                                                                         aTCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCa                 >  1:351500/1‑93 (MQ=255)
                                                                          tCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCAt                <  1:2030823/93‑1 (MQ=255)
                                                                          tCCCGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCAt                >  1:3915926/1‑93 (MQ=255)
                                                                           cccGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTTGCGGTACGTTACTTCATc               >  1:1949302/1‑93 (MQ=255)
                                                                           cccGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATc               <  2:1355694/93‑1 (MQ=255)
                                                                           cccGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATc               <  1:1115057/93‑1 (MQ=255)
                                                                            ccGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATcc              <  1:1174825/93‑1 (MQ=255)
                                                                             cGAAACCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCg             >  2:3005611/1‑93 (MQ=255)
                                                                                aaCCGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCaa          >  2:2165825/1‑93 (MQ=255)
                                                                                  ccGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAt        <  2:754844/93‑1 (MQ=255)
                                                                                   cGGAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAtg       <  1:583778/93‑1 (MQ=255)
                                                                                     gAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAtgtg     <  1:2464841/93‑1 (MQ=255)
                                                                                     gAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAtgtg     >  2:1861529/1‑93 (MQ=255)
                                                                                     gAAAGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAtgtg     <  1:4071135/93‑1 (MQ=255)
                                                                                      aaaGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTgg    <  2:1748440/93‑1 (MQ=255)
                                                                                       aaGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGc   >  2:503573/1‑93 (MQ=255)
                                                                                        aGTC‑TCCCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCt  <  1:1151909/93‑1 (MQ=255)
                                                                                              |                                                                                        
GATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCC‑CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCT  >  NC_000913/3481627‑3481806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCAT  >  NC_000913/3481633‑3481818
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GAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCCC                                                                                              <  SRR4302535.1933657/94‑1 (MQ=60)
 AACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCCcg                                                                                             <  SRR4302535.1034100/94‑3 (MQ=60)
  ACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCCcgc                                                                                            >  SRR4302535.1049268/1‑91 (MQ=60)
  ACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCCcgc                                                                                            >  SRR4302535.2098553/1‑91 (MQ=60)
    CGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAG                                                                                           >  SRR4302535.3189079/1‑94 (MQ=60)
      GATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGT                                                                                         <  SRR4302535.2705140/94‑1 (MQ=60)
      GATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGT                                                                                         <  SRR4302535.1792297/94‑1 (MQ=60)
      GATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGT                                                                                         <  SRR4302535.1617906/94‑1 (MQ=60)
      GATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGT                                                                                         <  SRR4302535.1574068/94‑1 (MQ=60)
         CGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGA                                                                                      >  SRR4302535.4335011/1‑94 (MQ=60)
          GCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAG                                                                                     <  SRR4302535.4186611/94‑1 (MQ=60)
          GCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAG                                                                                     >  SRR4302535.692128/1‑94 (MQ=60)
           CCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGT                                                                                    <  SRR4302535.4341640/94‑1 (MQ=60)
            CAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTA                                                                                   >  SRR4302535.1026395/1‑94 (MQ=60)
            CAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTA                                                                                   >  SRR4302535.4059189/1‑94 (MQ=60)
             AAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTAT                                                                                  >  SRR4302535.597772/1‑94 (MQ=60)
             AAACCGACGCTGATTATTCACCCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGCAACCGGAACGTC‑TCCCCCCCGCAGGTGGAGCAT                                                                                  >  SRR4302535.1317084/1‑94 (MQ=60)
              AACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGGTCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGGGGAGTGTC                                                                                 >  SRR4302535.3558732/1‑94 (MQ=60)
              AACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATC                                                                                 >  SRR4302535.3009120/1‑94 (MQ=60)
              AACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATC                                                                                 <  SRR4302535.2056672/94‑1 (MQ=60)
               ACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCA                                                                                >  SRR4302535.4688864/1‑94 (MQ=60)
                CCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAA                                                                               <  SRR4302535.2620505/94‑1 (MQ=60)
                CCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAA                                                                               <  SRR4302535.2613785/94‑1 (MQ=60)
                 CGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAAC                                                                              <  SRR4302535.4596360/94‑1 (MQ=60)
                 CGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAAC                                                                              >  SRR4302535.666150/1‑94 (MQ=60)
                  GTCGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACT                                                                             >  SRR4302535.4455605/1‑94 (MQ=60)
                  GACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACT                                                                             >  SRR4302535.3270564/1‑94 (MQ=60)
                  GACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACT                                                                             <  SRR4302535.1056900/94‑1 (MQ=60)
                  GACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACT                                                                             >  SRR4302535.4166040/1‑94 (MQ=60)
                  GACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTA‑TCCCCCCCGCAGGTGGAGTATCAACT                                                                             >  SRR4302535.4286171/1‑94 (MQ=60)
                     GCTGATTATTCACGCGAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCGC‑CCCCCCGCAGGTGGAGTATCAACTGAC                                                                          <  SRR4302535.3370607/94‑1 (MQ=60)
                     GCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGAC                                                                          <  SRR4302535.58673/94‑1 (MQ=60)
                     GCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGAC                                                                          >  SRR4302535.2392310/1‑94 (MQ=60)
                     GCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGAC                                                                          <  SRR4302535.1112292/94‑1 (MQ=60)
                      CTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACT                                                                         >  SRR4302535.2241008/1‑94 (MQ=60)
                      CTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACT                                                                         >  SRR4302535.3401799/1‑94 (MQ=60)
                      CTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACT                                                                         <  SRR4302535.2719961/94‑1 (MQ=60)
                      CTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACT                                                                         <  SRR4302535.1509030/94‑1 (MQ=60)
                       TGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTG                                                                        >  SRR4302535.811294/1‑94 (MQ=60)
                       TCATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCAC‑CCCCCCGCAGGTGGAGTATCAACTGACTG                                                                        <  SRR4302535.4167838/94‑1 (MQ=60)
                        GATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGA                                                                       >  SRR4302535.488120/1‑94 (MQ=60)
                         ATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAA                                                                      >  SRR4302535.4557949/1‑94 (MQ=60)
                          TTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAAC                                                                     >  SRR4302535.1875240/1‑94 (MQ=60)
                          TTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAAC                                                                     >  SRR4302535.301785/1‑94 (MQ=60)
                           TATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACA                                                                    <  SRR4302535.3029362/94‑1 (MQ=60)
                            ATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACAT                                                                   <  SRR4302535.1138535/94‑1 (MQ=60)
                            ATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCCGCAGGTGGAGTATCAACTGACTGAACATG                                                                  >  SRR4302535.3482129/1‑94 (MQ=60)
                             TTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATG                                                                  >  SRR4302535.3697718/1‑94 (MQ=60)
                              TCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGG                                                                 <  SRR4302535.2604525/94‑1 (MQ=60)
                               CACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGC                                                                <  SRR4302535.59442/94‑1 (MQ=60)
                               CACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGC                                                                <  SRR4302535.4395642/94‑1 (MQ=60)
                                   CCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTC                                                            <  SRR4302535.56599/94‑1 (MQ=60)
                                    CAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCA                                                           >  SRR4302535.137839/1‑94 (MQ=60)
                                    CAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCA                                                           <  SRR4302535.1329707/94‑1 (MQ=60)
                                    CAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCA                                                           <  SRR4302535.74990/94‑1 (MQ=60)
                                    CAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCA                                                           >  SRR4302535.644006/1‑94 (MQ=60)
                                    CAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCA                                                           >  SRR4302535.1985640/1‑94 (MQ=60)
                                     AAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCAT                                                          >  SRR4302535.879246/1‑94 (MQ=60)
                                        GTCGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTT                                                       <  SRR4302535.2200168/94‑1 (MQ=60)
                                        GACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTT                                                       <  SRR4302535.1550964/94‑1 (MQ=60)
                                         ACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTG                                                      <  SRR4302535.3064372/94‑1 (MQ=60)
                                         ACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTG                                                      >  SRR4302535.1408290/1‑94 (MQ=60)
                                         ACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTG                                                      <  SRR4302535.1652658/94‑1 (MQ=60)
                                          CGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGG                                                     >  SRR4302535.3251632/1‑94 (MQ=60)
                                           GATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGC                                                    >  SRR4302535.1065492/1‑94 (MQ=60)
                                           GATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGC                                                    >  SRR4302535.3288607/1‑94 (MQ=60)
                                            ATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCTACTGACTGAACATGGCGGTCATGTTGGCT                                                   <  SRR4302535.22854/94‑1 (MQ=60)
                                            ATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCT                                                   >  SRR4302535.2075111/1‑94 (MQ=60)
                                              CCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTT                                                 <  SRR4302535.658919/94‑1 (MQ=60)
                                              CCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTT                                                 <  SRR4302535.3000153/94‑1 (MQ=60)
                                              CCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTT                                                 <  SRR4302535.3850836/94‑1 (MQ=60)
                                                GTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTAT                                               >  SRR4302535.2633002/1‑94 (MQ=60)
                                                GTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTAT                                               >  SRR4302535.740549/1‑94 (MQ=60)
                                                  TTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTGTTG                                             >  SRR4302535.4576994/1‑94 (MQ=60)
                                                  TTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTG                                             >  SRR4302535.4244553/1‑94 (MQ=60)
                                                  TTATGGATCATCAGCTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTG                                             <  SRR4302535.2991402/94‑1 (MQ=60)
                                                   TATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGG                                            >  SRR4302535.3888038/1‑94 (MQ=60)
                                                   TATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGG                                            <  SRR4302535.2284732/94‑1 (MQ=60)
                                                    ATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGC                                           <  SRR4302535.1872081/94‑1 (MQ=60)
                                                    ATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGC                                           >  SRR4302535.3311554/1‑94 (MQ=60)
                                                     TGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCG                                          <  SRR4302535.29525/94‑1 (MQ=60)
                                                     TGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCG                                          <  SRR4302535.3619888/94‑1 (MQ=60)
                                                     TGGATCATCAGGGGAGCCCGAAACCGGAAAGTCGC‑CCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCG                                          <  SRR4302535.1027590/94‑1 (MQ=60)
                                                      GGATCATCTGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGG                                         <  SRR4302535.114272/94‑1 (MQ=60)
                                                       GATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGT                                        >  SRR4302535.2873667/1‑94 (MQ=60)
                                                        ATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTA                                       >  SRR4302535.155585/1‑94 (MQ=60)
                                                        ATCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGAGGTCATGTTGGCTTTATTGGCGGGA                                       >  SRR4302535.4538927/1‑94 (MQ=60)
                                                         TCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTAC                                      >  SRR4302535.4634536/1‑94 (MQ=60)
                                                         TCATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTAA                                      >  SRR4302535.2782508/1‑94 (MQ=60)
                                                          CATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACA                                     <  SRR4302535.1218239/94‑1 (MQ=60)
                                                          CATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACA                                     >  SRR4302535.2419089/1‑94 (MQ=60)
                                                           ATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACAT                                    >  SRR4302535.333143/1‑94 (MQ=60)
                                                           ATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACAT                                    <  SRR4302535.928040/94‑1 (MQ=60)
                                                           ATCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACAT                                    >  SRR4302535.4710586/1‑94 (MQ=60)
                                                            TCAGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATT                                   <  SRR4302535.1341281/94‑1 (MQ=60)
                                                            TCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTA                                  >  SRR4302535.4113223/1‑94 (MQ=60)
                                                              AGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTAC                                 <  SRR4302535.971283/94‑1 (MQ=60)
                                                              AGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTAC                                 >  SRR4302535.1456589/1‑94 (MQ=60)
                                                              AGGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTAC                                 <  SRR4302535.1497276/94‑1 (MQ=60)
                                                              AGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACT                                >  SRR4302535.1318022/1‑94 (MQ=60)
                                                               GGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACT                                >  SRR4302535.2101944/1‑94 (MQ=60)
                                                               GGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACT                                >  SRR4302535.776725/1‑94 (MQ=60)
                                                               GGTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACT                                <  SRR4302535.1527236/94‑1 (MQ=60)
                                                                GTGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTT                               >  SRR4302535.172055/1‑94 (MQ=60)
                                                                 TGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTC                              <  SRR4302535.2384737/94‑1 (MQ=60)
                                                                 TGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTC                              <  SRR4302535.143720/94‑1 (MQ=60)
                                                                 TGATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTC                              <  SRR4302535.1438985/94‑1 (MQ=60)
                                                                  GATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCA                             >  SRR4302535.1390114/1‑94 (MQ=60)
                                                                  GATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCA                             >  SRR4302535.353922/1‑94 (MQ=60)
                                                                   ATCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCAT                            >  SRR4302535.3942724/1‑94 (MQ=60)
                                                                    TCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTTGCGGTACGTTACTTCATC                           >  SRR4302535.1963334/1‑94 (MQ=60)
                                                                    TCCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATC                           <  SRR4302535.2045459/94‑1 (MQ=60)
                                                                     CCCGAAGCCGGAAAGGC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCC                          <  SRR4302535.567189/94‑1 (MQ=60)
                                                                     CCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCC                          >  SRR4302535.1264857/1‑94 (MQ=60)
                                                                     CCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCC                          <  SRR4302535.921036/94‑1 (MQ=60)
                                                                     CCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCC                          <  SRR4302535.4403799/94‑1 (MQ=60)
                                                                     CCCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCC                          <  SRR4302535.1123004/94‑1 (MQ=60)
                                                                      CCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCG                         <  SRR4302535.1183211/94‑1 (MQ=60)
                                                                      CCGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCG                         >  SRR4302535.2316397/1‑94 (MQ=60)
                                                                       CGAAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGC                        >  SRR4302535.1181271/1‑94 (MQ=60)
                                                                         AAACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAA                      >  SRR4302535.1965419/1‑94 (MQ=60)
                                                                          AACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAT                     >  SRR4302535.669775/1‑94 (MQ=60)
                                                                           ACCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAT                    <  SRR4302535.4376404/94‑1 (MQ=60)
                                                                            CCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATG                   <  SRR4302535.506849/94‑1 (MQ=60)
                                                                            CCGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATG                   >  SRR4302535.4473087/1‑94 (MQ=60)
                                                                             CGGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGT                  <  SRR4302535.587872/94‑1 (MQ=60)
                                                                              GGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTG                 <  SRR4302535.3646269/94‑1 (MQ=60)
                                                                              GGAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTG                 >  SRR4302535.4206205/1‑94 (MQ=60)
                                                                               GAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGG                <  SRR4302535.2482615/94‑1 (MQ=60)
                                                                               GAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGG                <  SRR4302535.4098995/94‑1 (MQ=60)
                                                                               GAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGG                <  SRR4302535.2841201/94‑1 (MQ=60)
                                                                               GAAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTAAATTTCTTCATCCCCAAATGGGG                >  SRR4302535.1111924/1‑94 (MQ=60)
                                                                                AAAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGGTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGC               <  SRR4302535.697561/94‑1 (MQ=60)
                                                                                 AAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCT              <  SRR4302535.355983/94‑1 (MQ=60)
                                                                                 AAGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCT              <  SRR4302535.3623886/94‑1 (MQ=60)
                                                                                  AGTC‑TCCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTG             <  SRR4302535.1160137/94‑1 (MQ=60)
                                                                                      ctcCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAG          >  SRR4302535.3413659/4‑94 (MQ=60)
                                                                                      ctcCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAG          >  SRR4302535.2798381/4‑94 (MQ=60)
                                                                                       tcCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGT         >  SRR4302535.4325405/3‑94 (MQ=60)
                                                                                          CCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCAC      <  SRR4302535.599348/94‑1 (MQ=60)
                                                                                           CCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACG     >  SRR4302535.1318295/1‑94 (MQ=60)
                                                                                            CCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGC    >  SRR4302535.4548266/1‑94 (MQ=60)
                                                                                            CCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGC    >  SRR4302535.2963740/1‑94 (MQ=60)
                                                                                              CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCAT  <  SRR4302535.4639646/94‑1 (MQ=60)
                                                                                              CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCAT  <  SRR4302535.3825018/94‑1 (MQ=60)
                                                                                              CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCAT  <  SRR4302535.1965870/94‑1 (MQ=60)
                                                                                              CGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCAAGCAT  >  SRR4302535.2375517/1‑94 (MQ=60)
                                                                                              |                                                                                             
GAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTC‑T‑CCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAATGTGGCTGGAGTCACGCAT  >  NC_000913/3481633‑3481818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑