Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,586,561 T→C N97S (AAC→AGC)  ugpC ← ATP‑binding component of sn‑glycerol 3‑phosphate transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,586,5610TC99.5% 688.5 / ‑5.0 219N97S (AAC→AGC) ugpCATP‑binding component of sn‑glycerol 3‑phosphate transport system
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (117/101);  total (117/102)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

Only 100 of 227 total aligned reads displayed.

CGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGC  >  NC_000913/3586471‑3586650
                                                                                          |                                                                                         
cgcTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTc                                                                                         >  2:2261475/1‑93 (MQ=255)
cgcTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTc                                                                                         <  2:3465295/93‑1 (MQ=255)
   tCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTgg                                                                                      <  2:3859320/93‑1 (MQ=255)
       cAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAaca                                                                                  >  2:1460526/1‑93 (MQ=255)
        aaTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAacac                                                                                 >  1:2880009/1‑93 (MQ=255)
        aaTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAacac                                                                                 >  2:517637/1‑93 (MQ=255)
        aaTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAacac                                                                                 >  1:1795343/1‑93 (MQ=255)
        aaTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAacac                                                                                 <  1:2615669/93‑1 (MQ=255)
         aTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAacaca                                                                                >  2:2740002/1‑92 (MQ=255)
         aTTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACAcc                                                                                >  1:1198076/1‑93 (MQ=255)
          tttGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCa                                                                               >  2:1715486/1‑93 (MQ=255)
           ttGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCAt                                                                              >  2:3327914/1‑93 (MQ=255)
           ttGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCAt                                                                              <  2:2454744/93‑1 (MQ=255)
           ttGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCAt                                                                              <  2:2330993/93‑1 (MQ=255)
           ttGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCAt                                                                              >  2:1006650/1‑93 (MQ=255)
                gctTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCaa                                                                         >  2:3171273/1‑93 (MQ=255)
                 ctTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAAt                                                                        >  2:3371661/1‑93 (MQ=255)
                 ctTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAAt                                                                        <  2:4653198/93‑1 (MQ=255)
                  ttGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATc                                                                       >  1:715668/1‑93 (MQ=255)
                   tGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATcc                                                                      <  1:1617888/93‑1 (MQ=255)
                     cccATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACCCCATCGCAATCCcg                                                                    >  2:220041/1‑93 (MQ=255)
                     cccATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCcg                                                                    <  1:3453604/93‑1 (MQ=255)
                         tGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGAt                                                                <  1:2297628/93‑1 (MQ=255)
                           ccGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCt                                                              <  1:4187077/93‑1 (MQ=255)
                            cgcgAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCtt                                                             >  2:1785405/1‑93 (MQ=255)
                            cgcgAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCtt                                                             <  2:3945531/93‑1 (MQ=255)
                             gcgAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCttt                                                            <  2:4206559/93‑1 (MQ=255)
                             gcgAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCttt                                                            <  2:2964254/93‑1 (MQ=255)
                             gcgAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCttt                                                            <  1:4224357/93‑1 (MQ=255)
                             gcgAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCttt                                                            >  2:1086549/1‑93 (MQ=255)
                              cgAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCtttt                                                           <  1:600822/93‑1 (MQ=255)
                               gAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTg                                                          <  1:2660667/93‑1 (MQ=255)
                               gAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTg                                                          >  1:4653280/1‑93 (MQ=255)
                               gAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTg                                                          <  1:1317592/93‑1 (MQ=255)
                                  ttttCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCt                                                       >  1:242877/1‑93 (MQ=255)
                                     tCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCa                                                    >  1:511495/1‑93 (MQ=255)
                                     tCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGCAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCa                                                    <  1:2510398/93‑1 (MQ=255)
                                      cAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCAt                                                   <  2:3086222/93‑1 (MQ=255)
                                       aGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCAtt                                                  <  1:2255681/93‑1 (MQ=255)
                                         cccccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTc                                                >  2:828247/1‑93 (MQ=255)
                                          ccccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCa                                               >  2:3534974/1‑93 (MQ=255)
                                          ccccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCa                                               >  1:521411/1‑93 (MQ=255)
                                          ccccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCACGCGATCTTTTGGCTCCATTTCa                                               <  2:4342014/93‑1 (MQ=255)
                                           cccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAg                                              >  2:1609048/1‑93 (MQ=255)
                                           cccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAg                                              <  1:3625630/93‑1 (MQ=255)
                                           cccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAg                                              >  2:863575/1‑93 (MQ=255)
                                            ccACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGt                                             >  2:631149/1‑93 (MQ=255)
                                             cACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTc                                            >  1:1449729/1‑93 (MQ=255)
                                              aCGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCa                                           <  2:4012804/93‑1 (MQ=255)
                                              aCGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCa                                           >  2:1728027/1‑93 (MQ=255)
                                               cGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAc                                          <  2:2772178/93‑1 (MQ=255)
                                                gCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcg                                         >  2:587061/1‑93 (MQ=255)
                                                 ccATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcgc                                        <  1:3889100/93‑1 (MQ=255)
                                                 ccATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCAcgc                                        <  2:858875/93‑1 (MQ=255)
                                                   aTGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACgcgc                                      <  1:210582/93‑1 (MQ=255)
                                                   aTGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACgcgc                                      <  2:673050/93‑1 (MQ=255)
                                                   aTGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACgcgc                                      <  2:2091567/93‑1 (MQ=255)
                                                    tGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCt                                     >  2:1418447/1‑93 (MQ=255)
                                                    tGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCt                                     >  1:3796593/1‑93 (MQ=255)
                                                    tGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCt                                     >  1:4003455/1‑93 (MQ=255)
                                                    tGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCt                                     <  1:1885970/93‑1 (MQ=255)
                                                      ttttCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTgg                                   <  2:4156244/93‑1 (MQ=255)
                                                       tttCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGt                                  >  1:2708860/1‑93 (MQ=255)
                                                         tcttcGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGGCg                                >  2:5500/1‑93 (MQ=255)
                                                          cttcGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGt                               >  2:204880/1‑93 (MQ=255)
                                                           ttcGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGtt                              <  1:371563/93‑1 (MQ=255)
                                                            tcGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGTTCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTg                             <  2:1335148/93‑1 (MQ=255)
                                                            tcGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTg                             <  1:3093335/93‑1 (MQ=255)
                                                              gACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGAt                           <  1:4318623/93‑1 (MQ=255)
                                                                 acTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATcta                        >  1:1532686/1‑91 (MQ=255)
                                                                 acTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCa                        >  2:3337943/1‑93 (MQ=255)
                                                                 acTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCa                        <  1:28306/93‑1 (MQ=255)
                                                                  cTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAg                       >  1:685201/1‑93 (MQ=255)
                                                                  cTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAg                       >  1:1036215/1‑93 (MQ=255)
                                                                   tCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGa                      <  1:905889/93‑1 (MQ=255)
                                                                   tCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGa                      <  2:3598275/93‑1 (MQ=255)
                                                                    cATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGat                     <  2:4544648/93‑1 (MQ=255)
                                                                     atatGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGata                    >  1:3204900/1‑93 (MQ=255)
                                                                     atatGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGata                    >  2:4088244/1‑93 (MQ=255)
                                                                      tatGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGatat                   >  2:2372610/1‑93 (MQ=255)
                                                                      tatGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGatat                   >  1:3383126/1‑93 (MQ=255)
                                                                      tatGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGatat                   >  1:192909/1‑93 (MQ=255)
                                                                       atGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATc                  >  1:3563865/1‑93 (MQ=255)
                                                                       atGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATc                  <  1:2956373/93‑1 (MQ=255)
                                                                        tGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCg                 >  1:317738/1‑93 (MQ=255)
                                                                        tGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCg                 >  2:2688606/1‑93 (MQ=255)
                                                                        tGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCg                 >  1:1213811/1‑93 (MQ=255)
                                                                        tGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTCGATCCAGATATCg                 >  2:2104615/1‑93 (MQ=255)
                                                                            gATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCtt             >  2:1672228/1‑93 (MQ=255)
                                                                             aTAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTc            <  1:564754/93‑1 (MQ=255)
                                                                              tAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCt           <  2:3745053/93‑1 (MQ=255)
                                                                              tAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCt           >  2:11208/1‑93 (MQ=255)
                                                                              tAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCt           <  2:4217060/93‑1 (MQ=255)
                                                                              tAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCt           <  2:177579/93‑1 (MQ=255)
                                                                                   cgcgTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCAc      <  2:179445/93‑1 (MQ=255)
                                                                                    gcgTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCAcc     <  1:1141040/93‑1 (MQ=255)
                                                                                     cgTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCAccc    <  2:3239556/93‑1 (MQ=255)
                                                                                      gTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCg   >  1:3017519/1‑93 (MQ=255)
                                                                                      gTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCg   >  1:4486580/1‑93 (MQ=255)
                                                                                      gTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCg   <  2:1776600/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGC  >  NC_000913/3586471‑3586650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCA  >  NC_000913/3586469‑3586654
                                                                                            |                                                                                             
CGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCT                                                                                              <  SRR4302535.196101/94‑1 (MQ=60)
CGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCT                                                                                              <  SRR4302535.3065890/94‑1 (MQ=60)
CGAGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCT                                                                                              <  SRR4302535.3680433/94‑1 (MQ=60)
  CGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCT                                                                                            <  SRR4302535.298328/94‑1 (MQ=60)
    CTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGG                                                                                          >  SRR4302535.4473467/1‑94 (MQ=60)
    CTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGG                                                                                          <  SRR4302535.3627191/94‑1 (MQ=60)
     TCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGA                                                                                         >  SRR4302535.3541247/1‑94 (MQ=60)
         CAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACAC                                                                                     >  SRR4302535.1808323/1‑94 (MQ=60)
         CAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACAC                                                                                     >  SRR4302535.2900399/1‑94 (MQ=60)
          AATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACC                                                                                    >  SRR4302535.1206657/1‑94 (MQ=60)
          AATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACC                                                                                    >  SRR4302535.4183223/1‑94 (MQ=60)
          AATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACC                                                                                    <  SRR4302535.2634426/94‑1 (MQ=60)
          AATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACC                                                                                    <  SRR4302535.3396363/94‑1 (MQ=60)
           ATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCA                                                                                   <  SRR4302535.976991/94‑1 (MQ=60)
           ATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCA                                                                                   <  SRR4302535.4690775/94‑1 (MQ=60)
            TTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCAT                                                                                  <  SRR4302535.1395600/94‑1 (MQ=60)
             TTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATC                                                                                 <  SRR4302535.4162877/94‑1 (MQ=60)
                CTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCA                                                                              >  SRR4302535.366413/1‑94 (MQ=60)
                CTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCA                                                                              >  SRR4302535.2448809/1‑94 (MQ=60)
                CTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCA                                                                              >  SRR4302535.2246416/1‑94 (MQ=60)
                CTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACAATGGCA                                                                              >  SRR4302535.1060048/1‑94 (MQ=60)
                 TGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAA                                                                             >  SRR4302535.4266357/1‑94 (MQ=60)
                   CTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATC                                                                           >  SRR4302535.720695/1‑94 (MQ=60)
                   CTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATC                                                                           >  SRR4302535.570998/1‑94 (MQ=60)
                     TGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCC                                                                         <  SRR4302535.1629541/94‑1 (MQ=60)
                     TGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCC                                                                         >  SRR4302535.2700659/1‑94 (MQ=60)
                       CCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGC                                                                       <  SRR4302535.3477611/94‑1 (MQ=60)
                        CCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCG                                                                      >  SRR4302535.1854426/1‑94 (MQ=60)
                          ATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGAT                                                                    >  SRR4302535.3659027/1‑94 (MQ=60)
                           TGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATC                                                                   <  SRR4302535.3840443/94‑1 (MQ=60)
                           TGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATC                                                                   <  SRR4302535.2314070/94‑1 (MQ=60)
                             CCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTT                                                                 <  SRR4302535.4215732/94‑1 (MQ=60)
                              CGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTT                                                                >  SRR4302535.3044091/1‑94 (MQ=60)
                               GCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTT                                                               <  SRR4302535.1922053/94‑1 (MQ=60)
                               GCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTT                                                               <  SRR4302535.4253262/94‑1 (MQ=60)
                                CGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTG                                                              >  SRR4302535.4684784/1‑94 (MQ=60)
                                CGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTG                                                              <  SRR4302535.605024/94‑1 (MQ=60)
                                CGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTG                                                              <  SRR4302535.4652207/94‑1 (MQ=60)
                                 GAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGG                                                             <  SRR4302535.1327005/94‑1 (MQ=60)
                                 GAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGG                                                             <  SRR4302535.2679726/94‑1 (MQ=60)
                                 GAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGG                                                             >  SRR4302535.121868/1‑94 (MQ=60)
                                   ATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCT                                                           >  SRR4302535.244539/1‑94 (MQ=60)
                                   ATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCT                                                           <  SRR4302535.1510211/94‑1 (MQ=60)
                                    TTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTC                                                          <  SRR4302535.315463/94‑1 (MQ=60)
                                      TTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCA                                                        >  SRR4302535.515070/1‑94 (MQ=60)
                                      TTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCA                                                        >  SRR4302535.2646711/1‑94 (MQ=60)
                                       TCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCAT                                                       <  SRR4302535.545408/94‑1 (MQ=60)
                                       TCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGCAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCAT                                                       <  SRR4302535.2528496/94‑1 (MQ=60)
                                         AGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTT                                                     <  SRR4302535.531314/94‑1 (MQ=60)
                                         AGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTT                                                     <  SRR4302535.2271740/94‑1 (MQ=60)
                                           CCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCA                                                   >  SRR4302535.525058/1‑94 (MQ=60)
                                            CCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAG                                                  <  SRR4302535.238843/94‑1 (MQ=60)
                                             CCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCACTCCCGCGATCTTTTGGCTCCATTTCAGC                                                 >  SRR4302535.621269/1‑94 (MQ=60)
                                             CCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGT                                                 <  SRR4302535.3650758/94‑1 (MQ=60)
                                             CCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGT                                                 >  SRR4302535.4231107/1‑94 (MQ=60)
                                              CCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTC                                                >  SRR4302535.1460097/1‑94 (MQ=60)
                                               CACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCA                                               >  SRR4302535.4026969/1‑94 (MQ=60)
                                               CACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCA                                               >  SRR4302535.1287359/1‑94 (MQ=60)
                                                   CCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCG                                           <  SRR4302535.3915768/94‑1 (MQ=60)
                                                    CATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGagt                                          >  SRR4302535.3413830/1‑91 (MQ=60)
                                                    CATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGC                                          >  SRR4302535.1694927/1‑94 (MQ=60)
                                                    CATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGC                                          >  SRR4302535.2941930/1‑94 (MQ=60)
                                                     ATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCT                                         <  SRR4302535.212029/94‑1 (MQ=60)
                                                     ATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCT                                         <  SRR4302535.2571599/94‑1 (MQ=60)
                                                     ATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCT                                         >  SRR4302535.4030839/1‑94 (MQ=60)
                                                     ATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCT                                         >  SRR4302535.3822766/1‑94 (MQ=60)
                                                      TGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTG                                        >  SRR4302535.1150443/1‑94 (MQ=60)
                                                      TGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTG                                        <  SRR4302535.1899556/94‑1 (MQ=60)
                                                      TGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTG                                        <  SRR4302535.2766079/94‑1 (MQ=60)
                                                        TTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGT                                      >  SRR4302535.2728220/1‑94 (MQ=60)
                                                        TTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGT                                      <  SRR4302535.860779/94‑1 (MQ=60)
                                                            CTTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTT                                  >  SRR4302535.691717/1‑94 (MQ=60)
                                                             TTCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTG                                 <  SRR4302535.374134/94‑1 (MQ=60)
                                                              TCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGA                                <  SRR4302535.3114877/94‑1 (MQ=60)
                                                              TCGACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGA                                >  SRR4302535.131607/1‑94 (MQ=60)
                                                                GACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATC                              <  SRR4302535.4348167/94‑1 (MQ=60)
                                                                 ACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCC                             >  SRR4302535.519963/1‑94 (MQ=60)
                                                                 ACACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCC                             >  SRR4302535.731665/1‑94 (MQ=60)
                                                                  CACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCTA                            >  SRR4302535.1543728/1‑94 (MQ=60)
                                                                  CACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCA                            >  SRR4302535.2452479/1‑94 (MQ=60)
                                                                  CACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCA                            >  SRR4302535.4528083/1‑94 (MQ=60)
                                                                   ACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAG                           >  SRR4302535.1043573/1‑94 (MQ=60)
                                                                   ACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAG                           >  SRR4302535.690004/1‑94 (MQ=60)
                                                                   ACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAG                           >  SRR4302535.2833606/1‑94 (MQ=60)
                                                                   ACTCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAG                           <  SRR4302535.28529/94‑1 (MQ=60)
                                                                     TCATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGAT                         <  SRR4302535.912317/94‑1 (MQ=60)
                                                                      CATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATA                        <  SRR4302535.2851732/94‑1 (MQ=60)
                                                                      CATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATA                        >  SRR4302535.3227096/1‑94 (MQ=60)
                                                                       ATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATAT                       >  SRR4302535.194238/1‑94 (MQ=60)
                                                                       ATATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATAT                       >  SRR4302535.3406646/1‑94 (MQ=60)
                                                                        TATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATC                      >  SRR4302535.3588576/1‑94 (MQ=60)
                                                                         ATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCG                     >  SRR4302535.1222517/1‑94 (MQ=60)
                                                                         ATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCG                     <  SRR4302535.2977138/94‑1 (MQ=60)
                                                                         ATGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCG                     >  SRR4302535.319942/1‑94 (MQ=60)
                                                                          TGCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGC                    <  SRR4302535.2052106/94‑1 (MQ=60)
                                                                           GCGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCC                   <  SRR4302535.3794630/94‑1 (MQ=60)
                                                                            CGGATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCT                  <  SRR4302535.15997/94‑1 (MQ=60)
                                                                               ATAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCT               <  SRR4302535.568708/94‑1 (MQ=60)
                                                                                TAAAGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTG              <  SRR4302535.2175642/94‑1 (MQ=60)
                                                                                   AGCGCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCA           >  SRR4302535.320665/1‑94 (MQ=60)
                                                                                      GCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCC        <  SRR4302535.3326192/94‑1 (MQ=60)
                                                                                      GCGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCC        <  SRR4302535.1149172/94‑1 (MQ=60)
                                                                                       CGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCG       >  SRR4302535.3054699/1‑94 (MQ=60)
                                                                                       CGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCG       >  SRR4302535.3038659/1‑94 (MQ=60)
                                                                                       CGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCG       >  SRR4302535.1660337/1‑94 (MQ=60)
                                                                                       CGTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCG       >  SRR4302535.4517380/1‑94 (MQ=60)
                                                                                        GTAGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGC      >  SRR4302535.227520/1‑94 (MQ=60)
                                                                                          AGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTC    >  SRR4302535.448705/1‑94 (MQ=60)
                                                                                          AGCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTC    >  SRR4302535.4043171/1‑94 (MQ=60)
                                                                                           GCTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCC   <  SRR4302535.3472036/94‑1 (MQ=60)
                                                                                            CTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCA  >  SRR4302535.3781112/1‑94 (MQ=60)
                                                                                            |                                                                                             
CGCGCTCGGCAATTTGCTGCTTGCCCATGCCGCGAATTTTCAGCCCCCACGCCATGTTTTCTTCGACACTCATATGCGGATAAAGCGCGTAGTTCTGGAACACCATCGCAATCCCGCGATCTTTTGGCTCCATTTCAGTCACGCGCTGGTCGTTGATCCAGATATCGCCTTCTGTCACCCGCTCCA  >  NC_000913/3586469‑3586654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑