Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,590,502 A→G T189T (ACT→ACC)  b3454 ← ATP‑binding component of leucine transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,590,5020AG100.0% 815.3 / NA 253T189T (ACT→ACC) b3454ATP‑binding component of leucine transport
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (95/158);  total (95/158)

Only 100 of 254 total aligned reads displayed.

ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591
                                                                                          |                                                                                         
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         >  1:3411609/1‑93 (MQ=255)
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         <  1:2561092/93‑1 (MQ=255)
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         >  2:1708608/1‑93 (MQ=255)
aTCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGt                                                                                         <  1:968787/93‑1 (MQ=255)
 tCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGta                                                                                        >  1:1641533/1‑92 (MQ=255)
 tCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTc                                                                                        <  2:326670/93‑1 (MQ=255)
  cGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCa                                                                                       <  1:1497202/93‑1 (MQ=255)
    gAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATc                                                                                     <  2:4088168/93‑1 (MQ=255)
    gAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATc                                                                                     >  2:2275980/1‑93 (MQ=255)
    gAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGGTGGCGTTCTGCTCGACGAGAAAGATGGTCATc                                                                                     >  1:2396484/1‑93 (MQ=255)
     aaaGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATcc                                                                                    <  2:1075294/93‑1 (MQ=255)
      aaGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATccc                                                                                   >  1:307928/1‑93 (MQ=255)
      aaGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATccc                                                                                   >  1:4367995/1‑93 (MQ=255)
          aCTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGc                                                                               >  1:2223978/1‑93 (MQ=255)
           cTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCt                                                                              >  2:838401/1‑93 (MQ=255)
           cTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCt                                                                              >  1:1031131/1‑93 (MQ=255)
            tACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTc                                                                             >  1:962129/1‑93 (MQ=255)
              cATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcgc                                                                           <  2:1956931/93‑1 (MQ=255)
              cATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTcgc                                                                           <  2:3796574/93‑1 (MQ=255)
                  gCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAg                                                                       >  1:2610960/1‑93 (MQ=255)
                   ccGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgc                                                                      <  1:1137999/93‑1 (MQ=255)
                   ccGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgc                                                                      >  1:2812240/1‑93 (MQ=255)
                    cGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgct                                                                     >  1:425487/1‑93 (MQ=255)
                    cGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgct                                                                     <  1:4160936/93‑1 (MQ=255)
                      ttttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCACCTGCTCGCGCAgctgc                                                                   >  2:2866424/1‑93 (MQ=255)
                       tttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgct                                                                  >  2:4648175/1‑93 (MQ=255)
                       tttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAgctgct                                                                  >  2:3747752/1‑93 (MQ=255)
                        ttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTc                                                                 <  2:1158037/93‑1 (MQ=255)
                        ttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTc                                                                 <  2:1180969/93‑1 (MQ=255)
                        ttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTc                                                                 <  1:2712954/93‑1 (MQ=255)
                        ttCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTc                                                                 <  1:3903976/93‑1 (MQ=255)
                           cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAt                                                              <  2:2218010/93‑1 (MQ=255)
                           cAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAt                                                              <  2:3135610/93‑1 (MQ=255)
                            aGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCCGCTGCTCGATg                                                             >  2:367729/1‑93 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            <  1:4602993/93‑1 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            <  2:4425644/93‑1 (MQ=255)
                             gCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATgg                                                            <  1:1281506/93‑1 (MQ=255)
                              cACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgt                                                           <  1:3629337/93‑1 (MQ=255)
                              cACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgt                                                           <  1:806124/93‑1 (MQ=255)
                               aCGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtg                                                          <  1:1851219/93‑1 (MQ=255)
                                cGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGgtgt                                                         <  2:2081846/93‑1 (MQ=255)
                                 gTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTc                                                        >  2:1100419/1‑93 (MQ=255)
                                     ccGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGaaa                                                    <  2:646681/93‑1 (MQ=255)
                                     ccGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGaaa                                                    >  1:2331263/1‑93 (MQ=255)
                                      cgcgATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGaaaa                                                   <  1:4091954/93‑1 (MQ=255)
                                        cgATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAtt                                                 <  2:3372887/93‑1 (MQ=255)
                                         gATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAttt                                                >  2:2639779/1‑93 (MQ=255)
                                            ccGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCt                                             <  2:418285/93‑1 (MQ=255)
                                              gCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTgg                                           <  2:3631501/93‑1 (MQ=255)
                                              gCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTgg                                           <  1:486730/93‑1 (MQ=255)
                                              gCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTgg                                           >  1:4231015/1‑93 (MQ=255)
                                               ccAGCTTTAGCGTCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          <  1:2036825/93‑1 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTTCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          <  1:1158216/93‑1 (MQ=255)
                                               ccAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGga                                          >  1:1631671/1‑93 (MQ=255)
                                                cAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgat                                         <  2:4255073/93‑1 (MQ=255)
                                                   cTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatgat                                      <  2:3240134/93‑1 (MQ=255)
                                                   cTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatgat                                      <  1:2788245/93‑1 (MQ=255)
                                                   cTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGgatgat                                      <  2:1561941/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTTATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  2:4512356/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  2:4372931/93‑1 (MQ=255)
                                                       aGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  2:3619814/93‑1 (MQ=255)
                                                       aGCGCCTGATTGGCGTTCTGCTCGACGAGAAAGATGGTCACCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATc                                  <  1:3234347/93‑1 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 <  1:3025582/93‑1 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 >  1:3324702/1‑93 (MQ=255)
                                                        gcgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCg                                 >  2:3468032/1‑93 (MQ=255)
                                                         cgcCTGGTTGGGGTTCTGCTCGATGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCgg                                <  2:3001730/93‑1 (MQ=255)
                                                         cgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCgg                                <  2:1591751/93‑1 (MQ=255)
                                                         cgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCgg                                <  2:3994169/93‑1 (MQ=255)
                                                         cgcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCgg                                <  1:178253/93‑1 (MQ=255)
                                                          gcCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgc                               >  2:1245110/1‑93 (MQ=255)
                                                           ccTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgcg                              >  2:38333/1‑93 (MQ=255)
                                                            cTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGgcgc                             >  1:4636999/1‑93 (MQ=255)
                                                             tGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCa                            >  2:1202955/1‑93 (MQ=255)
                                                             tGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCa                            <  2:236790/93‑1 (MQ=255)
                                                                 tGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAc                        <  1:3966639/93‑1 (MQ=255)
                                                                  ggCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAcc                       >  2:1267112/1‑93 (MQ=255)
                                                                   gCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCg                      <  2:1415989/93‑1 (MQ=255)
                                                                   gCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCg                      <  2:1901279/93‑1 (MQ=255)
                                                                    cGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGa                     <  2:3095661/93‑1 (MQ=255)
                                                                    cGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGa                     <  1:2098410/93‑1 (MQ=255)
                                                                      ttCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGc                   <  2:712754/93‑1 (MQ=255)
                                                                      ttCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGc                   <  2:4326724/93‑1 (MQ=255)
                                                                      ttCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGc                   <  2:2415143/93‑1 (MQ=255)
                                                                        cTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGa                 <  1:13620/93‑1 (MQ=255)
                                                                        cTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGa                 <  1:3951473/93‑1 (MQ=255)
                                                                        cTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGa                 <  2:565397/93‑1 (MQ=255)
                                                                        cTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGa                 <  1:61872/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  1:713763/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  1:3371336/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  2:1397628/93‑1 (MQ=255)
                                                                         tGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAt                <  2:4621591/93‑1 (MQ=255)
                                                                          gCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATg               <  1:3845617/93‑1 (MQ=255)
                                                                           cTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATgg              <  2:4030887/93‑1 (MQ=255)
                                                                            tCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGc             <  1:3551599/93‑1 (MQ=255)
                                                                                 gagaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCtcatc        >  1:2670816/1‑93 (MQ=255)
                                                                                   gaAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCaa      <  2:3293133/93‑1 (MQ=255)
                                                                                     aaGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGc    <  1:715633/93‑1 (MQ=255)
                                                                                      aGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGCAAATTTGCTGGATGATAATAGGCGCAAGACCGAGCGATGGCTCATCCAGCa   >  2:781139/1‑93 (MQ=255)
                                                                                      aGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCa   <  1:4513239/93‑1 (MQ=255)
                                                                                       gATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAg  <  1:3155169/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG  >  NC_000913/3590412‑3590591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGC  >  NC_000913/3590409‑3590595
                                                                                             |                                                                                             
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATG                                                                                               <  SRR4302535.1646370/94‑1 (MQ=60)
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATG                                                                                               >  SRR4302535.278162/1‑94 (MQ=60)
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATG                                                                                               <  SRR4302535.4589773/94‑1 (MQ=60)
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATG                                                                                               >  SRR4302535.332639/1‑94 (MQ=60)
  TATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGT                                                                                             >  SRR4302535.3435346/1‑94 (MQ=60)
   ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTC                                                                                            <  SRR4302535.2579530/94‑1 (MQ=60)
   ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTC                                                                                            <  SRR4302535.975669/94‑1 (MQ=60)
   ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTC                                                                                            >  SRR4302535.556799/1‑94 (MQ=60)
   ATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTC                                                                                            >  SRR4302535.3507306/1‑94 (MQ=60)
   AGCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTC                                                                                            <  SRR4302535.3360289/94‑1 (MQ=60)
     CGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                          <  SRR4302535.2936901/94‑1 (MQ=60)
     CGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCAT                                                                                          <  SRR4302535.1507969/94‑1 (MQ=60)
      GGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGGTGGCGTTCTGCTCGACGAGAAAGATGGTCATC                                                                                         >  SRR4302535.2413709/1‑94 (MQ=60)
        AAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCC                                                                                       >  SRR4302535.310059/1‑94 (MQ=60)
        AAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCC                                                                                       >  SRR4302535.4397885/1‑94 (MQ=60)
        AAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCC                                                                                       <  SRR4302535.115591/94‑1 (MQ=60)
          AGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCT                                                                                     <  SRR4302535.3409587/94‑1 (MQ=60)
           GCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTG                                                                                    <  SRR4302535.4345032/94‑1 (MQ=60)
            CACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGC                                                                                   >  SRR4302535.2239807/1‑94 (MQ=60)
             ACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCT                                                                                  >  SRR4302535.2678721/1‑94 (MQ=60)
             ACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCT                                                                                  >  SRR4302535.1038455/1‑94 (MQ=60)
              CTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTC                                                                                 >  SRR4302535.968964/1‑94 (MQ=60)
               TACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCG                                                                                >  SRR4302535.271256/1‑94 (MQ=60)
                   TGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCA                                                                            <  SRR4302535.311478/94‑1 (MQ=60)
                    GGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAG                                                                           <  SRR4302535.4145395/94‑1 (MQ=60)
                    GGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAG                                                                           >  SRR4302535.2629693/1‑94 (MQ=60)
                     GCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGC                                                                          >  SRR4302535.2832226/1‑94 (MQ=60)
                      CCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCT                                                                         <  SRR4302535.156050/94‑1 (MQ=60)
                      CCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCT                                                                         >  SRR4302535.428453/1‑94 (MQ=60)
                      CCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCT                                                                         <  SRR4302535.282478/94‑1 (MQ=60)
                      CCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCT                                                                         <  SRR4302535.1146115/94‑1 (MQ=60)
                       CGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTG                                                                        <  SRR4302535.4189416/94‑1 (MQ=60)
                        GTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGC                                                                       >  SRR4302535.4513339/1‑94 (MQ=60)
                        GTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGC                                                                       <  SRR4302535.1105497/94‑1 (MQ=60)
                         TTTTCCAGCACGTAGCCGCGATCAGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCT                                                                      <  SRR4302535.2127638/94‑1 (MQ=60)
                          TTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCCGCTCGCGCAGCTGCTC                                                                     <  SRR4302535.3071831/94‑1 (MQ=60)
                           TTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCG                                                                    <  SRR4302535.3930719/94‑1 (MQ=60)
                           TTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCG                                                                    <  SRR4302535.4266427/94‑1 (MQ=60)
                           TTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCG                                                                    <  SRR4302535.2732339/94‑1 (MQ=60)
                             CCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAT                                                                  <  SRR4302535.719105/94‑1 (MQ=60)
                             CCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAT                                                                  >  SRR4302535.942205/1‑94 (MQ=60)
                             CCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGAT                                                                  <  SRR4302535.886629/94‑1 (MQ=60)
                              CAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATG                                                                 <  SRR4302535.3021186/94‑1 (MQ=60)
                              CAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATG                                                                 <  SRR4302535.4390157/94‑1 (MQ=60)
                              CAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATG                                                                 <  SRR4302535.450771/94‑1 (MQ=60)
                               AGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGG                                                                >  SRR4302535.1967976/1‑94 (MQ=60)
                               AGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGG                                                                <  SRR4302535.2511285/94‑1 (MQ=60)
                                GCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGT                                                               <  SRR4302535.4634293/94‑1 (MQ=60)
                                GCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGT                                                               <  SRR4302535.1290652/94‑1 (MQ=60)
                                GCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTATGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGT                                                               <  SRR4302535.4564773/94‑1 (MQ=60)
                                 CACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTG                                                              <  SRR4302535.811779/94‑1 (MQ=60)
                                 CACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTG                                                              <  SRR4302535.3654493/94‑1 (MQ=60)
                                  ACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGT                                                             <  SRR4302535.1864557/94‑1 (MQ=60)
                                   CGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTTGATGGTGTG                                                            >  SRR4302535.1201597/1‑94 (MQ=60)
                                      AGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAA                                                         >  SRR4302535.1328877/1‑94 (MQ=60)
                                      AGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAA                                                         >  SRR4302535.133544/1‑94 (MQ=60)
                                       GCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAA                                                        >  SRR4302535.2347936/1‑94 (MQ=60)
                                         CGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCGCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAT                                                      <  SRR4302535.3969556/94‑1 (MQ=60)
                                         CGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAT                                                      >  SRR4302535.4244360/1‑94 (MQ=60)
                                         CGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAT                                                      <  SRR4302535.4119953/94‑1 (MQ=60)
                                         CGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAAT                                                      >  SRR4302535.383296/1‑94 (MQ=60)
                                          GCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATT                                                     <  SRR4302535.1847935/94‑1 (MQ=60)
                                           CGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTT                                                    >  SRR4302535.968968/1‑94 (MQ=60)
                                           CGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTT                                                    >  SRR4302535.733094/1‑94 (MQ=60)
                                             ATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGC                                                  >  SRR4302535.4240124/1‑94 (MQ=60)
                                               CCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTG                                                >  SRR4302535.20796/1‑94 (MQ=60)
                                                CGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGG                                               >  SRR4302535.4259968/1‑94 (MQ=60)
                                                 GCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGA                                              >  SRR4302535.1643459/1‑94 (MQ=60)
                                                 GCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGA                                              <  SRR4302535.490143/94‑1 (MQ=60)
                                                  CCAGCTTTAGCGTCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                             <  SRR4302535.2051492/94‑1 (MQ=60)
                                                  CCAGCTTTAGCGCCTGGTTGGCGTTCTTCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                             <  SRR4302535.1166497/94‑1 (MQ=60)
                                                  CCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGAT                                             <  SRR4302535.1154343/94‑1 (MQ=60)
                                                   CAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATG                                            <  SRR4302535.2540556/94‑1 (MQ=60)
                                                   CAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATG                                            <  SRR4302535.399015/94‑1 (MQ=60)
                                                      CTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATA                                         <  SRR4302535.2808087/94‑1 (MQ=60)
                                                      CTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATA                                         <  SRR4302535.4009170/94‑1 (MQ=60)
                                                       TTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAA                                        <  SRR4302535.38756/94‑1 (MQ=60)
                                                        TTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAAT                                       <  SRR4302535.1893627/94‑1 (MQ=60)
                                                        TTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAAT                                       <  SRR4302535.719313/94‑1 (MQ=60)
                                                         TAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATC                                      <  SRR4302535.47536/94‑1 (MQ=60)
                                                         TAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATC                                      <  SRR4302535.1688011/94‑1 (MQ=60)
                                                          AGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                     <  SRR4302535.4662137/94‑1 (MQ=60)
                                                          AGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                     >  SRR4302535.3347837/1‑94 (MQ=60)
                                                          AGCGCCTGATTGGCGTTCTGCTCGACGAGAAAGATGGTCACCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCG                                     <  SRR4302535.3256790/94‑1 (MQ=60)
                                                           GCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGG                                    <  SRR4302535.3046763/94‑1 (MQ=60)
                                                            CGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGC                                   <  SRR4302535.179492/94‑1 (MQ=60)
                                                            CGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGC                                   <  SRR4302535.4073535/94‑1 (MQ=60)
                                                            CGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGC                                   <  SRR4302535.2394020/94‑1 (MQ=60)
                                                             GCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCG                                  >  SRR4302535.1550941/1‑94 (MQ=60)
                                                             GCCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCG                                  >  SRR4302535.3786490/1‑94 (MQ=60)
                                                              CCTGGTTGGCGTTCTGCTCGACGAGAACGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGC                                 <  SRR4302535.345385/94‑1 (MQ=60)
                                                              CCTGGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGC                                 >  SRR4302535.4668452/1‑94 (MQ=60)
                                                                 GGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAG                              <  SRR4302535.2742624/94‑1 (MQ=60)
                                                                 GGTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGGGTCGAAAATTTGCTGGATGATAATCGGCGCACG                              >  SRR4302535.2740018/1‑94 (MQ=60)
                                                                  GTTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGA                             >  SRR4302535.69065/1‑94 (MQ=60)
                                                                   TTGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGAC                            <  SRR4302535.3943256/94‑1 (MQ=60)
                                                                    TGGCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACC                           <  SRR4302535.3993732/94‑1 (MQ=60)
                                                                      GCGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATTGTGTCGAAAATTTGCTGGATGATAATTGGCGCAAGAACGA                         >  SRR4302535.3300340/1‑94 (MQ=60)
                                                                       CGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAG                        <  SRR4302535.2113545/94‑1 (MQ=60)
                                                                       CGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAG                        <  SRR4302535.1664291/94‑1 (MQ=60)
                                                                       CGTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAACACCGAG                        >  SRR4302535.1440979/1‑94 (MQ=60)
                                                                        GTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGC                       <  SRR4302535.3234112/94‑1 (MQ=60)
                                                                        GTTCTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGC                       >  SRR4302535.3345758/1‑94 (MQ=60)
                                                                           CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    <  SRR4302535.3978452/94‑1 (MQ=60)
                                                                           CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    <  SRR4302535.13720/94‑1 (MQ=60)
                                                                           CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    <  SRR4302535.558025/94‑1 (MQ=60)
                                                                           CTGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGAT                    <  SRR4302535.62335/94‑1 (MQ=60)
                                                                            TGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATG                   <  SRR4302535.2323291/94‑1 (MQ=60)
                                                                            TGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATG                   <  SRR4302535.3394791/94‑1 (MQ=60)
                                                                            TGCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATG                   <  SRR4302535.718778/94‑1 (MQ=60)
                                                                             GCTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGG                  <  SRR4302535.3872095/94‑1 (MQ=60)
                                                                              CTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGC                 >  SRR4302535.22217/1‑94 (MQ=60)
                                                                              CTCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGC                 <  SRR4302535.3103963/94‑1 (MQ=60)
                                                                               TCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCT                <  SRR4302535.3628799/94‑1 (MQ=60)
                                                                               TCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCT                <  SRR4302535.3576245/94‑1 (MQ=60)
                                                                               TCGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCT                >  SRR4302535.1651873/1‑94 (MQ=60)
                                                                                CGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTC               <  SRR4302535.2302671/94‑1 (MQ=60)
                                                                                CGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTC               >  SRR4302535.4646870/1‑94 (MQ=60)
                                                                                CGACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTC               <  SRR4302535.267403/94‑1 (MQ=60)
                                                                                  ACGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCAT             >  SRR4302535.2584953/1‑94 (MQ=60)
                                                                                   CGAGAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATC            >  SRR4302535.2689925/1‑94 (MQ=60)
                                                                                      GAAAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAG         <  SRR4302535.3378565/94‑1 (MQ=60)
                                                                                        AAGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCA       <  SRR4302535.720660/94‑1 (MQ=60)
                                                                                         AGATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAG      <  SRR4302535.4544186/94‑1 (MQ=60)
                                                                                          GATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGT     <  SRR4302535.3177085/94‑1 (MQ=60)
                                                                                           ATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTA    <  SRR4302535.2236981/94‑1 (MQ=60)
                                                                                           ATGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTA    >  SRR4302535.2189569/1‑94 (MQ=60)
                                                                                            TGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGCAAATTTGCTGGATGATGATCGAAGCAAGACCGAGCGATGGCTATTCAAGCAGTAG   >  SRR4302535.899215/1‑94 (MQ=60)
                                                                                            TGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAG   <  SRR4302535.4158543/94‑1 (MQ=60)
                                                                                            TGGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAG   <  SRR4302535.1429894/94‑1 (MQ=60)
                                                                                             GGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGC  >  SRR4302535.3101502/1‑94 (MQ=60)
                                                                                             GGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGC  <  SRR4302535.19303/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGTCATCCCCTGCTCGCGCAGCTGCTCGATGGTGTCGAAAATTTGCTGGATGATAATCGGCGCAAGACCGAGCGATGGCTCATCAAGCAGTAGC  >  NC_000913/3590409‑3590595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑