Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,627,142 +G intergenic (‑24/+23) yhiH ← / ← yhiI putative ATP‑binding component of a transport system, fragment 1/putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,627,1401.G100.0% 728.7 / NA 231intergenic (‑22/+25)yhiH/yhiIputative ATP‑binding component of a transport system, fragment 1/putative membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (137/94);  total (137/94)

Only 100 of 256 total aligned reads displayed.

ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227
                                                                                             |                                                                                       
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                        <  2:2865494/93‑1 (MQ=255)
  cATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gg                                                                                       <  1:1162730/93‑1 (MQ=255)
  cATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gg                                                                                       >  1:63578/1‑93 (MQ=255)
     aCAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggac                                                                                    <  1:3199352/93‑4 (MQ=255)
      cAGCGGGCCGGAATATCGAGACTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggacg                                                                                   <  1:1055507/93‑5 (MQ=255)
        gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACggg                                                                                  >  1:1025767/1‑93 (MQ=255)
        gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACggg                                                                                  >  1:2048590/1‑93 (MQ=255)
             ccGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCa                                                                             <  2:3337905/93‑1 (MQ=255)
             ccGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCa                                                                             <  1:3855134/93‑1 (MQ=255)
              cGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAg                                                                            <  2:3406925/93‑1 (MQ=255)
              cGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGCTGCGCGACAGGCGGGACGGGAACCAg                                                                            >  1:386250/1‑93 (MQ=255)
               ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGt                                                                           >  2:1840633/1‑93 (MQ=255)
               ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGt                                                                           >  1:3634780/1‑93 (MQ=255)
               ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGt                                                                           <  1:1337038/93‑1 (MQ=255)
                gAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                          >  1:3046058/1‑93 (MQ=255)
                gAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                          >  1:3951999/1‑93 (MQ=255)
                gAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                          >  1:225735/1‑93 (MQ=255)
                gAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                          >  2:4278829/1‑93 (MQ=255)
                gAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                          <  2:792405/93‑1 (MQ=255)
                 aaTATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTc                                                                         >  1:2318253/1‑93 (MQ=255)
                  atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTcc                                                                        <  2:3719981/93‑1 (MQ=255)
                  atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTcc                                                                        >  2:3026092/1‑93 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCa                                                                       >  1:4500525/1‑93 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCa                                                                       >  2:3764339/1‑93 (MQ=255)
                     tCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGa                                                                     <  2:84931/93‑1 (MQ=255)
                      cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAt                                                                    >  1:1914183/1‑93 (MQ=255)
                         gagTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCg                                                                 >  1:48076/1‑93 (MQ=255)
                         gagTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCg                                                                 >  1:2618171/1‑93 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATGATGCTGGCTCACGCCCGCCTGTTGCGCGACAGGCGGGACGGGAGCCCGGTCCAGGTGCGTc                                                               >  2:3521640/1‑93 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCCGATGCGTc                                                               >  2:1525989/1‑93 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTc                                                               >  1:3425688/1‑93 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCACGGCAGATGCGCGACAGGCGGGACGGGAACCAGGTCCAGATGCGta                                                               >  1:2138003/1‑92 (MQ=255)
                             gATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                             >  1:4601564/1‑93 (MQ=255)
                             gATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                             >  1:2560943/1‑93 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAtt                                                            <  1:1506569/93‑1 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAtt                                                            >  2:709858/1‑93 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAtt                                                            >  1:3487323/1‑93 (MQ=255)
                                atTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGc                                                          <  1:4555307/93‑1 (MQ=255)
                                 ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCg                                                         >  1:3335044/1‑93 (MQ=255)
                                 ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCg                                                         >  2:305363/1‑93 (MQ=255)
                                  tGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCCGATGCGTCATTGCgg                                                        >  1:3144986/1‑93 (MQ=255)
                                   gTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGc                                                       <  1:84810/93‑1 (MQ=255)
                                    tttaGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCa                                                      <  1:4249042/90‑1 (MQ=255)
                                    ttCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCa                                                      >  1:2964259/1‑93 (MQ=255)
                                      cAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAc                                                    <  1:3541220/93‑1 (MQ=255)
                                         cgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTc                                                 >  1:3609695/1‑93 (MQ=255)
                                           cAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcac                                               >  1:4491221/1‑93 (MQ=255)
                                           cAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcac                                               >  1:3244676/1‑93 (MQ=255)
                                            aaCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacc                                              >  2:1802951/1‑93 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             >  1:288973/1‑93 (MQ=255)
                                             aCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             >  1:681242/1‑93 (MQ=255)
                                              cGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcaccac                                            <  1:1239729/93‑1 (MQ=255)
                                               ggTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACg                                           >  1:3732388/1‑93 (MQ=255)
                                                gTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGa                                          >  2:3975136/1‑93 (MQ=255)
                                                 tttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                         <  2:904835/93‑1 (MQ=255)
                                                   tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAggc                                       >  1:2761571/1‑92 (MQ=255)
                                                     tCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcg                                     >  1:1191343/1‑93 (MQ=255)
                                                     tCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcg                                     >  2:1337601/1‑93 (MQ=255)
                                                      ccATAATGTTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgt                                    >  2:4567215/1‑93 (MQ=255)
                                                       cATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgtc                                   >  2:2104809/1‑93 (MQ=255)
                                                        aTACTGCTGGCGCCCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCCCCACGGGGTCGTCa                                  <  1:2403144/93‑1 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  >  2:1226162/1‑93 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  <  1:3397098/93‑1 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  >  2:260624/1‑93 (MQ=255)
                                                         tAATGCTGGCCCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                 <  1:2207919/93‑1 (MQ=255)
                                                          aaTGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAgg                                >  1:4514756/1‑93 (MQ=255)
                                                           aTGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGc                               >  1:953678/1‑93 (MQ=255)
                                                           aTGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGc                               >  1:4014065/1‑93 (MQ=255)
                                                            tGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGcc                              >  2:2947764/1‑93 (MQ=255)
                                                            tGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGcc                              >  1:3954899/1‑93 (MQ=255)
                                                              cTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCAc                            <  2:3101882/93‑1 (MQ=255)
                                                               tGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACg                           >  2:535186/1‑93 (MQ=255)
                                                               tGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACg                           >  2:942024/1‑93 (MQ=255)
                                                                ggCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGTAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACgg                          >  1:3991156/1‑93 (MQ=255)
                                                                 gCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGa                         <  2:3225241/93‑1 (MQ=255)
                                                                    cACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGt                      <  2:1065883/93‑1 (MQ=255)
                                                                      cgcccgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                    <  2:3184457/93‑1 (MQ=255)
                                                                      cgcccgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                    <  2:170132/93‑1 (MQ=255)
                                                                        cccgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttctt                  >  1:4667370/1‑93 (MQ=255)
                                                                         ccgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttcttc                 <  2:3312933/93‑1 (MQ=255)
                                                                          cgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttca                <  1:1623312/93‑1 (MQ=255)
                                                                          cgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttca                >  2:1987629/1‑93 (MQ=255)
                                                                           gccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcat               >  2:2141659/1‑93 (MQ=255)
                                                                           gccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcat               >  2:4379979/1‑93 (MQ=255)
                                                                           gccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcat               >  1:1985705/1‑93 (MQ=255)
                                                                            ccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcatt              >  2:4499870/1‑93 (MQ=255)
                                                                            ccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcatt              <  2:137768/93‑1 (MQ=255)
                                                                              aGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattca            <  1:79748/93‑1 (MQ=255)
                                                                              aGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattca            >  1:1843603/1‑93 (MQ=255)
                                                                               gTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           >  1:3705403/1‑93 (MQ=255)
                                                                               gTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           >  2:541896/1‑93 (MQ=255)
                                                                               gTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           <  2:2237028/93‑1 (MQ=255)
                                                                               gTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           <  1:414392/93‑1 (MQ=255)
                                                                               gTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           >  2:3653503/1‑93 (MQ=255)
                                                                                ttGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAcc          <  1:464235/93‑1 (MQ=255)
                                                                                  gcgcGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGGAGTTCTTCATTCACCCg        >  1:1735002/1‑93 (MQ=255)
                                                                                   cgcgACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGc       <  2:2483234/93‑1 (MQ=255)
                                                                                    gcgACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCa      <  2:1952174/93‑1 (MQ=255)
                                                                                      gACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAcc    >  2:4220098/1‑93 (MQ=255)
                                                                                      gACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAcc    <  2:3888541/93‑1 (MQ=255)
                                                                                             |                                                                                       
ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG  >  NC_000913/3627049‑3627234
                                                                                              |                                                                                            
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                              >  SRR4302535.1843196/1‑94 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                              >  SRR4302535.64057/1‑94 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                             <  SRR4302535.1171039/94‑1 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                             <  SRR4302535.4588996/94‑1 (MQ=60)
   TACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgac                                                                                           <  SRR4302535.307822/94‑4 (MQ=60)
    ACAGCGGGCCGTAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                           <  SRR4302535.910106/94‑1 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                           <  SRR4302535.3925215/94‑1 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                           <  SRR4302535.713192/94‑1 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                           <  SRR4302535.3221498/94‑1 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                           >  SRR4302535.2782594/1‑94 (MQ=60)
     CAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGG                                                                                          >  SRR4302535.625941/1‑94 (MQ=60)
     CAGCGGGCCGGAATATCGAGACTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGG                                                                                          <  SRR4302535.1063011/94‑1 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGG                                                                                         >  SRR4302535.1205758/1‑94 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGG                                                                                         >  SRR4302535.2063339/1‑94 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGG                                                                                         >  SRR4302535.1033053/1‑94 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCAGAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGG                                                                                         <  SRR4302535.3324520/94‑1 (MQ=60)
         GGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGGCGGGAAC                                                                                      >  SRR4302535.3696623/1‑94 (MQ=60)
         GGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAAC                                                                                      <  SRR4302535.2692754/94‑1 (MQ=60)
         GGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAAC                                                                                      <  SRR4302535.3279927/94‑1 (MQ=60)
           GCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCA                                                                                    <  SRR4302535.245593/94‑1 (MQ=60)
           GCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCA                                                                                    >  SRR4302535.4222893/1‑94 (MQ=60)
            CCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAG                                                                                   <  SRR4302535.3881680/94‑1 (MQ=60)
            CCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAG                                                                                   <  SRR4302535.341642/94‑1 (MQ=60)
            CCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGCTGCGCGACAGGCGGGACGGGAACCAG                                                                                   >  SRR4302535.388934/1‑94 (MQ=60)
             CGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGT                                                                                  >  SRR4302535.3659967/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTT                                                                                 >  SRR4302535.3067394/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTT                                                                                 <  SRR4302535.1346576/94‑1 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTT                                                                                 >  SRR4302535.227287/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTT                                                                                 >  SRR4302535.3978981/1‑94 (MQ=60)
               GAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTC                                                                                >  SRR4302535.2334846/1‑94 (MQ=60)
                AATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCC                                                                               >  SRR4302535.2373303/1‑94 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCA                                                                              >  SRR4302535.3175053/1‑94 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCA                                                                              >  SRR4302535.261084/1‑94 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCA                                                                              >  SRR4302535.4531434/1‑94 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCA                                                                              >  SRR4302535.1406828/1‑94 (MQ=60)
                   ATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGA                                                                            <  SRR4302535.1089953/94‑1 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAT                                                                           >  SRR4302535.4641383/1‑94 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAT                                                                           >  SRR4302535.1927978/1‑94 (MQ=60)
                    TCGAGAGTGATATTGTCCAGCGCAACGGTTTTTCAATAATGCTGGATCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAT                                                                           <  SRR4302535.2234559/94‑1 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCG                                                                        >  SRR4302535.1182215/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCG                                                                        >  SRR4302535.2073134/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCG                                                                        >  SRR4302535.2636940/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCG                                                                        >  SRR4302535.48436/1‑94 (MQ=60)
                        GAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAAACAGTTCCAGActcgt                                                                       >  SRR4302535.2998737/1‑89 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTC                                                                      >  SRR4302535.214239/1‑94 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTC                                                                      >  SRR4302535.3449509/1‑94 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCACGGCAGATGCGCGACAGGCGGGACGGGAACCAGGTCCAGATGCGTA                                                                      >  SRR4302535.2153400/1‑94 (MQ=60)
                           TGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAT                                                                    >  SRR4302535.4632857/1‑94 (MQ=60)
                           TGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAT                                                                    >  SRR4302535.2579381/1‑94 (MQ=60)
                           TGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAT                                                                    <  SRR4302535.600489/94‑1 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGCACCAGTTTCAGATTCGTCATT                                                                   >  SRR4302535.1607653/1‑94 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                   <  SRR4302535.2666844/94‑1 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                   >  SRR4302535.3511535/1‑94 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                  <  SRR4302535.3481118/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                  <  SRR4302535.1517401/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                  >  SRR4302535.1702351/1‑94 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                  >  SRR4302535.677812/1‑94 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATTCGTCATTGCG                                                                >  SRR4302535.2520721/1‑94 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                <  SRR4302535.4526163/94‑1 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                >  SRR4302535.1657952/1‑94 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                <  SRR4302535.4586421/94‑1 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                >  SRR4302535.3358270/1‑94 (MQ=60)
                                TTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCCGATGCGTCATTGCGG                                                               >  SRR4302535.3166858/1‑94 (MQ=60)
                                 TGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGC                                                              >  SRR4302535.3956811/1‑94 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                             >  SRR4302535.2985063/1‑94 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                             <  SRR4302535.85407/94‑1 (MQ=60)
                                   TTTAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAA                                                            <  SRR4302535.4278143/94‑1 (MQ=60)
                                     CAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACC                                                          <  SRR4302535.3565826/94‑1 (MQ=60)
                                       GCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTC                                                        >  SRR4302535.3634731/1‑94 (MQ=60)
                                        CGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGATCCAGATGCGTCATTGCCGCAACCTCA                                                       >  SRR4302535.1150539/1‑94 (MQ=60)
                                         GCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAC                                                      >  SRR4302535.3267212/1‑94 (MQ=60)
                                         GCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAC                                                      >  SRR4302535.4522050/1‑94 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                     <  SRR4302535.191995/94‑1 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                     <  SRR4302535.4556806/94‑1 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                    >  SRR4302535.290995/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                    >  SRR4302535.3630738/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                    >  SRR4302535.2932009/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                    >  SRR4302535.686010/1‑94 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCA                                                    >  SRR4302535.3240607/1‑94 (MQ=60)
                                            ACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCAC                                                   <  SRR4302535.3732701/94‑1 (MQ=60)
                                            ACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCAC                                                   >  SRR4302535.3668608/1‑94 (MQ=60)
                                            ACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAATGCGGCAACCTCACCAC                                                   >  SRR4302535.4659419/1‑94 (MQ=60)
                                             CGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACG                                                  <  SRR4302535.4005725/94‑1 (MQ=60)
                                             CGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACG                                                  <  SRR4302535.1248578/94‑1 (MQ=60)
                                             CGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACG                                                  >  SRR4302535.3758230/1‑94 (MQ=60)
                                              GGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGA                                                 <  SRR4302535.4523953/94‑1 (MQ=60)
                                              GGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGA                                                 <  SRR4302535.3882457/94‑1 (MQ=60)
                                               GTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAG                                                <  SRR4302535.4266993/94‑1 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                               <  SRR4302535.4545794/94‑1 (MQ=60)
                                                 TTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGC                                              >  SRR4302535.2781250/1‑94 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                             <  SRR4302535.3551354/94‑1 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                             <  SRR4302535.2028750/94‑1 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                             >  SRR4302535.12408/1‑94 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                             <  SRR4302535.105745/94‑1 (MQ=60)
                                                   TTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCG                                            >  SRR4302535.1199873/1‑94 (MQ=60)
                                                    TCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGT                                           >  SRR4302535.3835729/1‑94 (MQ=60)
                                                    TCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGGCGT                                           >  SRR4302535.3342145/1‑94 (MQ=60)
                                                     CCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTTCGGCAACCTCACCACGAGGTCGTC                                          >  SRR4302535.1318047/1‑94 (MQ=60)
                                                     CCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTC                                          >  SRR4302535.3426311/1‑94 (MQ=60)
                                                     CCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTC                                          >  SRR4302535.4539146/1‑94 (MQ=60)
                                                       ATACTGCTGGCGCCCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCCCCACGGGGTCGTCAG                                        <  SRR4302535.2420431/94‑1 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                        <  SRR4302535.3420733/94‑1 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                        >  SRR4302535.4090991/1‑94 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                        <  SRR4302535.1792430/94‑1 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                        <  SRR4302535.1976934/94‑1 (MQ=60)
                                                       ATAATGCTGGCCCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCACCCTCACCACGAGGTCGTCAG                                        <  SRR4302535.4025484/94‑1 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                       >  SRR4302535.4545712/1‑94 (MQ=60)
                                                        TAATGCTGGCCCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                       <  SRR4302535.2223645/94‑1 (MQ=60)
                                                         AATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGC                                      >  SRR4302535.960434/1‑94 (MQ=60)
                                                         AATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGC                                      >  SRR4302535.4041520/1‑94 (MQ=60)
                                                         AATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGC                                      >  SRR4302535.2223240/1‑94 (MQ=60)
                                                         AATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGC                                      >  SRR4302535.2994688/1‑94 (MQ=60)
                                                          ATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCC                                     >  SRR4302535.3981899/1‑94 (MQ=60)
                                                           TGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCA                                    >  SRR4302535.4684588/1‑94 (MQ=60)
                                                           TGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAAGCGTCATGGCGGCAACCTCACCACGAGGACGTCAGGCCA                                    >  SRR4302535.4717470/1‑94 (MQ=60)
                                                              TGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGTAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGG                                 >  SRR4302535.4018454/1‑94 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAG                              <  SRR4302535.3530/94‑1 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGACGTCAGGCCACGGAAG                              >  SRR4302535.4696311/1‑94 (MQ=60)
                                                                  TCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGT                             <  SRR4302535.3652059/94‑1 (MQ=60)
                                                                   CACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTT                            <  SRR4302535.3982131/94‑1 (MQ=60)
                                                                   CACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTT                            <  SRR4302535.3099166/94‑1 (MQ=60)
                                                                     CGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCT                          <  SRR4302535.522102/94‑1 (MQ=60)
                                                                      GCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTT                         >  SRR4302535.4698913/1‑94 (MQ=60)
                                                                       CCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTC                        >  SRR4302535.4066100/1‑94 (MQ=60)
                                                                        CCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCA                       >  SRR4302535.80624/1‑94 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                      <  SRR4302535.4392944/94‑1 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                      <  SRR4302535.1635030/94‑1 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                      >  SRR4302535.2379379/1‑94 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                      >  SRR4302535.3429825/1‑94 (MQ=60)
                                                                         CGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCAT                      >  SRR4302535.2000005/1‑94 (MQ=60)
                                                                           CCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTC                    >  SRR4302535.387852/1‑94 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                   <  SRR4302535.777877/94‑1 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                   >  SRR4302535.1856897/1‑94 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                   >  SRR4302535.3201189/1‑94 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                  >  SRR4302535.3731062/1‑94 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                  <  SRR4302535.80316/94‑1 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                  >  SRR4302535.2266164/1‑94 (MQ=60)
                                                                              GTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACC                 <  SRR4302535.417303/94‑1 (MQ=60)
                                                                               TTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCC                <  SRR4302535.467480/94‑1 (MQ=60)
                                                                               TTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCC                <  SRR4302535.2924298/94‑1 (MQ=60)
                                                                                TGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGGAGTTCTTCATTCACCCG               >  SRR4302535.1747546/1‑94 (MQ=60)
                                                                                TGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCG               <  SRR4302535.363823/94‑1 (MQ=60)
                                                                                  CGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCA             >  SRR4302535.2304335/1‑94 (MQ=60)
                                                                                   GCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAC            <  SRR4302535.950816/94‑1 (MQ=60)
                                                                                    CGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACC           <  SRR4302535.3980851/94‑1 (MQ=60)
                                                                                       CAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC        >  SRR4302535.2899681/1‑94 (MQ=60)
                                                                                        AGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACG       >  SRR4302535.1663920/1‑94 (MQ=60)
                                                                                           cgGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTA    <  SRR4302535.4406639/92‑1 (MQ=60)
                                                                                             GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG  <  SRR4302535.1239849/94‑1 (MQ=60)
                                                                                              |                                                                                            
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG  >  NC_000913/3627049‑3627234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑