Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,658,084	T→C	V14A (GTA→GCA)	yhiV →	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,658,084	0	T	C	99.6%	742.6 / NA	234	V14A (GTA→GCA)	yhiV	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (130/103); minor base A (0/1); total (130/104)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

ACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTA  >  NC_000913/3657994‑3658173

 cGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACt                                                                                        >  1:517855/1‑93 (MQ=255)

  gCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACtt                                                                                       <  1:2846874/93‑1 (MQ=255)

  gCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACtt                                                                                       <  2:1579817/93‑1 (MQ=255)

  gCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACtt                                                                                       >  1:1901289/1‑93 (MQ=255)

    cAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGc                                                                                     >  2:4240812/1‑93 (MQ=255)

    cAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGc                                                                                     <  2:366597/93‑1 (MQ=255)

    cAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGc                                                                                     >  2:1511368/1‑93 (MQ=255)

    cAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGc                                                                                     >  2:4687259/1‑93 (MQ=255)

      gCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCa                                                                                   <  2:866613/93‑1 (MQ=255)

        aCCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCatt                                                                                 >  1:749635/1‑93 (MQ=255)

        aCCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCatt                                                                                 >  1:4377482/1‑93 (MQ=255)

         ccGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCatta                                                                                <  1:205613/93‑1 (MQ=255)

         ccGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCatta                                                                                >  1:2183422/1‑93 (MQ=255)

         ccGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCatta                                                                                >  2:698585/1‑93 (MQ=255)

          cGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCACGGTTTTTGCCTGGGCACTTGCCattat                                                                               <  2:674306/93‑1 (MQ=255)

           gAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCattatt                                                                              >  1:2970374/1‑93 (MQ=255)

            aaTCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCAttatta                                                                             <  2:2810659/93‑1 (MQ=255)

              tCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTatg                                                                           >  2:3875839/1‑93 (MQ=255)

              tCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTatg                                                                           <  2:3363663/93‑1 (MQ=255)

               cGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTatga                                                                          <  2:2630117/93‑1 (MQ=255)

                gAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTatgat                                                                         <  1:3861261/93‑1 (MQ=255)

                gAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTatgat                                                                         >  2:2499880/1‑93 (MQ=255)

                 aaaCAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTatgatg                                                                        <  1:3282474/93‑1 (MQ=255)

                  aaCAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGc                                                                       >  1:4273880/1‑93 (MQ=255)

                  aaCAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGc                                                                       >  2:3604380/1‑93 (MQ=255)

                   aCAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCt                                                                      >  1:4605994/1‑93 (MQ=255)

                   aCAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCt                                                                      <  1:4150512/93‑1 (MQ=255)

                    cAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCtt                                                                     <  1:1419853/93‑1 (MQ=255)

                    cAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCtt                                                                     >  1:4508079/1‑93 (MQ=255)

                      aTAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGc                                                                   >  2:2289951/1‑93 (MQ=255)

                      aTAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGc                                                                   <  1:1141490/93‑1 (MQ=255)

                       tAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCa                                                                  >  1:3092683/1‑93 (MQ=255)

                       tAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCa                                                                  >  2:2031279/1‑93 (MQ=255)

                        aaCGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAg                                                                 <  2:4493689/93‑1 (MQ=255)

                          cGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAggt                                                               >  1:2795171/1‑93 (MQ=255)

                              gCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTc                                                           <  1:951290/93‑1 (MQ=255)

                               cAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCt                                                          <  2:328488/93‑1 (MQ=255)

                                 ggCTGAAGGCGACTTTCATGGCTAACTAGCTTATTGATCGCCCGGTTTTTGCCTGGGAGCTTGCCATGATTATGATGCTTGCAGGTGGTCAgg                                                        <  2:4159094/93‑1 (MQ=255)

                                  gCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGc                                                       >  1:1116699/1‑93 (MQ=255)

                                    ttAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGa                                                     <  1:419069/93‑1 (MQ=255)

                                       aGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCa                                                  <  1:4066975/93‑1 (MQ=255)

                                       aGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCa                                                  >  1:3534858/1‑93 (MQ=255)

                                       aGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCa                                                  <  1:1666136/93‑1 (MQ=255)

                                        ggggACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCAt                                                 <  1:3781077/93‑1 (MQ=255)

                                         gggACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATg                                                >  2:4021885/1‑93 (MQ=255)

                                          ggACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGa                                               >  2:2967851/1‑93 (MQ=255)

                                           gACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGaa                                              <  1:3941381/93‑1 (MQ=255)

                                             cTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACt                                            >  2:1278352/1‑93 (MQ=255)

                                             cTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACt                                            >  2:3729186/1‑93 (MQ=255)

                                              tttCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACtt                                           >  1:4028539/1‑93 (MQ=255)

                                              tttCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACtt                                           >  1:1215781/1‑93 (MQ=255)

                                              tttCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACta                                           >  1:3818638/1‑92 (MQ=255)

                                                 cATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTaac                                        >  2:1744698/1‑91 (MQ=255)

                                                 cATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTAcc                                        <  2:429278/93‑1 (MQ=255)

                                                 cATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTAcc                                        <  1:863707/93‑1 (MQ=255)

                                                  aTGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCg                                       >  1:3994738/1‑93 (MQ=255)

                                                  aTGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCg                                       >  2:2732795/1‑93 (MQ=255)

                                                  aTGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCg                                       <  2:585850/93‑1 (MQ=255)

                                                   tGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCgg                                      >  2:3452985/1‑93 (MQ=255)

                                                   tGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCgg                                      >  1:24268/1‑93 (MQ=255)

                                                   tGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCgg                                      >  1:316636/1‑93 (MQ=255)

                                                    ggttAATTATTTTATTGACCGCCGGGTTTTTGCCTGGGCCCTTGCCTTTATTATAATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGt                                     <  2:3779713/90‑1 (MQ=255)

                                                     gCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGtt                                    <  2:3221851/93‑1 (MQ=255)

                                                      cTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTg                                   >  1:995557/1‑93 (MQ=255)

                                                       tAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTgc                                  >  1:46301/1‑93 (MQ=255)

                                                       tAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTgc                                  >  2:3408166/1‑93 (MQ=255)

                                                       tAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTgc                                  >  2:4473458/1‑93 (MQ=255)

                                                         aCTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTgcgc                                <  2:1353003/93‑1 (MQ=255)

                                                         aCTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTgcgc                                >  2:4368425/1‑93 (MQ=255)

                                                              tttATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTAGTCTGGCGATCATGAACTTACCGGTTGCGCAGTAt                           <  2:828402/93‑1 (MQ=255)

                                                               ttATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGACCTTACCGGTTGCGCAGTATc                          <  1:1164323/93‑1 (MQ=255)

                                                                 aTTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCg                        >  1:1046384/1‑93 (MQ=255)

                                                                  ttGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGc                       <  2:763726/93‑1 (MQ=255)

                                                                   tGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCa                      <  1:585342/93‑1 (MQ=255)

                                                                   tGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGAGATCATGAACTTACTGGTTGCGCAGTATCAGCa                      >  1:1570525/1‑93 (MQ=255)

                                                                    gATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAg                     >  2:2365042/1‑93 (MQ=255)

                                                                    gATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAg                     >  1:1742950/1‑93 (MQ=255)

                                                                     aTCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGa                    <  2:391387/93‑1 (MQ=255)

                                                                      tCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGAt                   >  2:1706359/1‑93 (MQ=255)

                                                                      tCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGAt                   >  1:2678529/1‑93 (MQ=255)

                                                                       cGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGAtt                  >  2:4608704/1‑93 (MQ=255)

                                                                       cGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGAtt                  <  1:2094871/93‑1 (MQ=255)

                                                                       cGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGAtt                  <  2:926436/93‑1 (MQ=255)

                                                                         cccGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTgc                <  1:812364/93‑1 (MQ=255)

                                                                          ccGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTgcg               >  1:2773184/1‑93 (MQ=255)

                                                                            ggTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGcc             <  1:2211277/93‑1 (MQ=255)

                                                                             gTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCa            >  1:2862036/1‑93 (MQ=255)

                                                                             gTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCa            >  1:2837265/1‑93 (MQ=255)

                                                                             gTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCa            >  2:314583/1‑93 (MQ=255)

                                                                              tttttGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCAc           >  2:3714868/1‑93 (MQ=255)

                                                                              tttttGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCAc           >  2:890846/1‑93 (MQ=255)

                                                                               ttttGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCAcc          <  2:629241/93‑1 (MQ=255)

                                                                               ttttGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCAcc          >  1:2325260/1‑93 (MQ=255)

                                                                                tttGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCg         <  1:698585/93‑1 (MQ=255)

                                                                                tttGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCg         >  2:1331626/1‑93 (MQ=255)

                                                                                    ccTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCa     <  1:4318402/93‑1 (MQ=255)

                                                                                    ccTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCa     <  2:2255487/93‑1 (MQ=255)

                                                                                     cTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGGCACCGACCAt    >  2:3539127/1‑93 (MQ=255)

                                                                                     cTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCAt    <  1:3431219/93‑1 (MQ=255)

                                                                                       gggCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTa  <  1:3672445/93‑1 (MQ=255)

ACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTA  >  NC_000913/3657994‑3658173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGT  >  NC_000913/3657991‑3658177

AAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGC                                                                                               <  SRR4302535.4221403/94‑1 (MQ=60)

  AACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCAC                                                                                             >  SRR4302535.4267995/1‑94 (MQ=60)

  AACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCAC                                                                                             >  SRR4302535.848590/1‑94 (MQ=60)

   ACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACT                                                                                            <  SRR4302535.2962115/94‑1 (MQ=60)

   ACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACT                                                                                            >  SRR4302535.521481/1‑94 (MQ=60)

    CGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTT                                                                                           >  SRR4302535.1914972/1‑94 (MQ=60)

     GCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTG                                                                                          <  SRR4302535.2296273/94‑1 (MQ=60)

     GCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTG                                                                                          <  SRR4302535.2867054/94‑1 (MQ=60)

      CCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGC                                                                                         >  SRR4302535.1551470/1‑94 (MQ=60)

      CCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGC                                                                                         <  SRR4302535.998531/94‑1 (MQ=60)

        AGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCTCCCGGTTTTTGCCTGGGCACTTGCCA                                                                                       <  SRR4302535.524328/94‑1 (MQ=60)

        AGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCA                                                                                       >  SRR4302535.4417444/1‑94 (MQ=60)

         GCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCAT                                                                                      <  SRR4302535.151739/94‑1 (MQ=60)

          CACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATT                                                                                     >  SRR4302535.4407435/1‑94 (MQ=60)

          CACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATT                                                                                     >  SRR4302535.754917/1‑94 (MQ=60)

           ACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTA                                                                                    >  SRR4302535.2198991/1‑94 (MQ=60)

            CCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTAT                                                                                   <  SRR4302535.207022/94‑1 (MQ=60)

             CGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATT                                                                                  >  SRR4302535.2991224/1‑94 (MQ=60)

             CGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATT                                                                                  <  SRR4302535.2094265/94‑1 (MQ=60)

               AATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTAT                                                                                >  SRR4302535.3266745/1‑94 (MQ=60)

               AATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTAT                                                                                <  SRR4302535.3417796/94‑1 (MQ=60)

               AATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTAGCCATTATTAT                                                                                >  SRR4302535.772151/1‑94 (MQ=60)

                ATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATG                                                                               <  SRR4302535.3817874/94‑1 (MQ=60)

                 TCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGA                                                                              >  SRR4302535.2857450/1‑94 (MQ=60)

                   GAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATG                                                                            <  SRR4302535.3887829/94‑1 (MQ=60)

                    AAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGC                                                                           >  SRR4302535.3520107/1‑94 (MQ=60)

                    AAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGC                                                                           <  SRR4302535.3305271/94‑1 (MQ=60)

                    AAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGC                                                                           >  SRR4302535.4303136/1‑94 (MQ=60)

                     AACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCT                                                                          >  SRR4302535.4637300/1‑94 (MQ=60)

                      ACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTT                                                                         >  SRR4302535.4025288/1‑94 (MQ=60)

                      ACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTT                                                                         <  SRR4302535.4178933/94‑1 (MQ=60)

                      ACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTT                                                                         >  SRR4302535.4539014/1‑94 (MQ=60)

                      ACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGGTTTTGCCTGGGCACTTGCCATTATTATGATGCTT                                                                         >  SRR4302535.4150048/1‑94 (MQ=60)

                       CAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTG                                                                        <  SRR4302535.1429991/94‑1 (MQ=60)

                        AATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGC                                                                       >  SRR4302535.1182941/1‑94 (MQ=60)

                         ATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCA                                                                      <  SRR4302535.3853058/94‑1 (MQ=60)

                         ATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCA                                                                      >  SRR4302535.3935527/1‑94 (MQ=60)

                         ATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCA                                                                      >  SRR4302535.3114218/1‑94 (MQ=60)

                         ATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCA                                                                      <  SRR4302535.3272234/94‑1 (MQ=60)

                         ATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCA                                                                      <  SRR4302535.1149627/94‑1 (MQ=60)

                         ATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCA                                                                      >  SRR4302535.1280113/1‑94 (MQ=60)

                          TAGCGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAG                                                                     <  SRR4302535.2873644/94‑1 (MQ=60)

                          TAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAG                                                                     <  SRR4302535.1863475/94‑1 (MQ=60)

                            ACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGT                                                                   >  SRR4302535.2815061/1‑94 (MQ=60)

                             CGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTG                                                                  >  SRR4302535.3982565/1‑94 (MQ=60)

                                TGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTC                                                               <  SRR4302535.4527736/94‑1 (MQ=60)

                                 GCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCT                                                              <  SRR4302535.958022/94‑1 (MQ=60)

                                 GCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCT                                                              <  SRR4302535.2847942/94‑1 (MQ=60)

                                   AGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGG                                                            <  SRR4302535.3629942/94‑1 (MQ=60)

                                   AGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGG                                                            >  SRR4302535.664254/1‑94 (MQ=60)

                                   AGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGG                                                            <  SRR4302535.3565111/94‑1 (MQ=60)

                                    GGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGC                                                           >  SRR4302535.1124662/1‑94 (MQ=60)

                                       TTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGAT                                                        <  SRR4302535.3777419/94‑1 (MQ=60)

                                       TTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGAT                                                        <  SRR4302535.422002/94‑1 (MQ=60)

                                         AAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCA                                                      <  SRR4302535.4353778/94‑1 (MQ=60)

                                         AAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCA                                                      >  SRR4302535.3559396/1‑94 (MQ=60)

                                          AGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCAT                                                     <  SRR4302535.1678190/94‑1 (MQ=60)

                                          AGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCAT                                                     <  SRR4302535.4625772/94‑1 (MQ=60)

                                          AGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCAT                                                     <  SRR4302535.4094804/94‑1 (MQ=60)

                                           GGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATG                                                    <  SRR4302535.3807173/94‑1 (MQ=60)

                                              GACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAAC                                                 <  SRR4302535.3968285/94‑1 (MQ=60)

                                               GCTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACT                                                <  SRR4302535.1601870/94‑1 (MQ=60)

                                                CTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTT                                               >  SRR4302535.4056101/1‑94 (MQ=60)

                                                CTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTT                                               >  SRR4302535.1224502/1‑94 (MQ=60)

                                                CTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTT                                               >  SRR4302535.2813667/1‑94 (MQ=60)

                                                CTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTA                                               >  SRR4302535.3844988/1‑94 (MQ=60)

                                                 TTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTA                                              <  SRR4302535.2107627/94‑1 (MQ=60)

                                                  TTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTAC                                             >  SRR4302535.219966/1‑94 (MQ=60)

                                                   TCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACC                                            <  SRR4302535.502466/94‑1 (MQ=60)

                                                    CATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCG                                           <  SRR4302535.4683063/94‑1 (MQ=60)

                                                    CATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCG                                           >  SRR4302535.4022069/1‑94 (MQ=60)

                                                    CATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCG                                           <  SRR4302535.869810/94‑1 (MQ=60)

                                                    CATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCG                                           <  SRR4302535.247620/94‑1 (MQ=60)

                                                     ATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGG                                          >  SRR4302535.24456/1‑94 (MQ=60)

                                                     ATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGG                                          >  SRR4302535.850140/1‑94 (MQ=60)

                                                     ATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGG                                          >  SRR4302535.318834/1‑94 (MQ=60)

                                                        GCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTG                                       >  SRR4302535.1705809/1‑94 (MQ=60)

                                                        GCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTG                                       >  SRR4302535.1002625/1‑94 (MQ=60)

                                                        GCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTG                                       >  SRR4302535.3987787/1‑94 (MQ=60)

                                                        GCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTG                                       >  SRR4302535.2717957/1‑94 (MQ=60)

                                                         CTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGG                                      >  SRR4302535.1073669/1‑94 (MQ=60)

                                                         CTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGC                                      >  SRR4302535.1170304/1‑94 (MQ=60)

                                                         CTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGC                                      >  SRR4302535.46651/1‑94 (MQ=60)

                                                           AACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGC                                    >  SRR4302535.464501/1‑94 (MQ=60)

                                                            ACTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCA                                   <  SRR4302535.1166248/94‑1 (MQ=60)

                                                             CTATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAG                                  >  SRR4302535.3823250/1‑94 (MQ=60)

                                                               ATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTA                                >  SRR4302535.3053063/1‑94 (MQ=60)

                                                               ATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTA                                <  SRR4302535.2815142/94‑1 (MQ=60)

                                                               ATTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTA                                >  SRR4302535.899502/1‑94 (MQ=60)

                                                                TTTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTAT                               >  SRR4302535.4288173/1‑94 (MQ=60)

                                                                 TTTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATC                              >  SRR4302535.3152673/1‑94 (MQ=60)

                                                                  TTATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGACCTTACCGGTTGCGCAGTATCC                             <  SRR4302535.1172637/94‑1 (MQ=60)

                                                                   TATTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCG                            >  SRR4302535.1053801/1‑94 (MQ=60)

                                                                     TTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCA                          >  SRR4302535.2987932/1‑94 (MQ=60)

                                                                     TTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCA                          <  SRR4302535.754062/94‑1 (MQ=60)

                                                                     TTGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGAGATCATGAACTTACTGGTTGCGCAGTATCAGCA                          >  SRR4302535.1581863/1‑94 (MQ=60)

                                                                      TGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAG                         >  SRR4302535.1755554/1‑94 (MQ=60)

                                                                      TGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAG                         <  SRR4302535.589444/94‑1 (MQ=60)

                                                                      TGATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAG                         <  SRR4302535.1967853/94‑1 (MQ=60)

                                                                        ATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGAT                       <  SRR4302535.4209429/94‑1 (MQ=60)

                                                                        ATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGAT                       >  SRR4302535.1140496/1‑94 (MQ=60)

                                                                        ATCGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGAT                       >  SRR4302535.2697691/1‑94 (MQ=60)

                                                                          CGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTG                     <  SRR4302535.2109977/94‑1 (MQ=60)

                                                                          CGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTG                     <  SRR4302535.2439802/94‑1 (MQ=60)

                                                                          CGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTG                     >  SRR4302535.4333132/1‑94 (MQ=60)

                                                                          CGCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTG                     <  SRR4302535.4147160/94‑1 (MQ=60)

                                                                           GCCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGC                    >  SRR4302535.4082708/1‑94 (MQ=60)

                                                                            CCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCG                   >  SRR4302535.681764/1‑94 (MQ=60)

                                                                            CCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCG                   <  SRR4302535.818060/94‑1 (MQ=60)

                                                                            CCCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCG                   >  SRR4302535.2792930/1‑94 (MQ=60)

                                                                             CCGGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGC                  >  SRR4302535.2195624/1‑94 (MQ=60)

                                                                               GGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCA                >  SRR4302535.2882310/1‑94 (MQ=60)

                                                                               GGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCA                <  SRR4302535.2227029/94‑1 (MQ=60)

                                                                               GGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCA                >  SRR4302535.2857399/1‑94 (MQ=60)

                                                                               GGTTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCA                >  SRR4302535.2235252/1‑94 (MQ=60)

                                                                                 TTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACC              <  SRR4302535.2781002/94‑1 (MQ=60)

                                                                                 TTTTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACC              >  SRR4302535.2341896/1‑94 (MQ=60)

                                                                                   TTTGCCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGA            <  SRR4302535.703486/94‑1 (MQ=60)

                                                                                       CCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCAT        <  SRR4302535.4347945/94‑1 (MQ=60)

                                                                                       CCTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCAT        >  SRR4302535.182866/1‑94 (MQ=60)

                                                                                        CTGGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATT       <  SRR4302535.3455066/94‑1 (MQ=60)

                                                                                          GGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTAC     <  SRR4302535.3697876/94‑1 (MQ=60)

                                                                                          GGGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTAC     >  SRR4302535.4168136/1‑94 (MQ=60)

                                                                                           GGCACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACC    >  SRR4302535.2761563/1‑94 (MQ=60)

                                                                                             CACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGT  >  SRR4302535.3242490/1‑94 (MQ=60)

                                                                                             CACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGT  >  SRR4302535.4156551/1‑94 (MQ=60)

                                                                                             CACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGT  >  SRR4302535.2003424/1‑94 (MQ=60)

                                                                                             CACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGT  >  SRR4302535.2228753/1‑94 (MQ=60)

                                                                                             CACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCACAGATTGCGCCACCGACCATTACCGT  <  SRR4302535.2141164/94‑1 (MQ=60)

AAAACGCCAGCACCGAATCGAAACAATAACGTTGCAGGCTTAAGGGGACTTTCATGGCTAACTATTTTATTGATCGCCCGGTTTTTGCCTGGGTACTTGCCATTATTATGATGCTTGCAGGTGGTCTGGCGATCATGAACTTACCGGTTGCGCAGTATCCGCAGATTGCGCCACCGACCATTACCGT  >  NC_000913/3657991‑3658177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`