Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,679,306 T→C S89G (AGC→GGC)  yhjJ ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,679,3060TC99.6% 727.7 / ‑5.0 236S89G (AGC→GGC) yhjJhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (109/126);  total (109/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

Only 100 of 247 total aligned reads displayed.

CATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCTTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAA  >  NC_000913/3679216‑3679395
                                                                                          |                                                                                         
aaTAAGAGACAATAACAGGCGGCATCGGGCGATTAGAGCAAAACCCCTGATGCCACAATGAACGCGCCTGTGCAGCGTCAAGGCCACCGCctt                                                                                         <  2:4681126/92‑1 (MQ=255)
   aaGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCg                                                                                      >  1:2850831/1‑93 (MQ=255)
   aaGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGAGCTCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCg                                                                                      >  2:19260/1‑93 (MQ=255)
    agagACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGt                                                                                     >  2:1212535/1‑93 (MQ=255)
     gagaCAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTACTGCGTCAAGGACACCGCCTTGCGtt                                                                                    >  2:501931/1‑93 (MQ=255)
      agaCAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTa                                                                                   <  2:4046781/93‑1 (MQ=255)
      agaCAATTACCAGCGGCATCGGTCTTTTAGGATCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTa                                                                                   <  1:1952001/93‑1 (MQ=255)
        aCAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAgc                                                                                 <  2:1358901/93‑1 (MQ=255)
        aCAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAgc                                                                                 <  1:2495531/93‑1 (MQ=255)
           aTTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCa                                                                              >  1:1782979/1‑93 (MQ=255)
           aTTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCa                                                                              >  1:3514887/1‑93 (MQ=255)
            ttACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCaa                                                                             >  2:1707203/1‑93 (MQ=255)
            ttACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCaa                                                                             <  1:2082597/93‑1 (MQ=255)
            ttACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCaa                                                                             >  2:3813939/1‑93 (MQ=255)
             tACCGGCGGCATGGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAAt                                                                            <  2:3938934/93‑1 (MQ=255)
               ccGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATAc                                                                          <  2:1462557/93‑1 (MQ=255)
               ccGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATAc                                                                          >  2:2187551/1‑93 (MQ=255)
                cggcggCATCGGGCTTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACg                                                                         <  1:2463267/93‑1 (MQ=255)
                 ggcggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGa                                                                        <  1:1196016/93‑1 (MQ=255)
                 ggcggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGa                                                                        >  2:2463099/1‑93 (MQ=255)
                   cggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAgg                                                                      <  1:565725/93‑1 (MQ=255)
                    ggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAggg                                                                     >  2:2663840/1‑93 (MQ=255)
                    ggcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAggg                                                                     <  2:2355902/93‑1 (MQ=255)
                    ggcATCGGGCGTTTAGGGTCGATCCCCTGCTG‑CACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    <  1:3555716/93‑1 (MQ=255)
                     gcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGGGGGa                                                                    >  2:691318/1‑93 (MQ=255)
                     gcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    <  2:4652898/93‑1 (MQ=255)
                     gcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    <  1:168250/93‑1 (MQ=255)
                     gcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    <  1:3862329/93‑1 (MQ=255)
                     gcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    <  2:3517829/93‑1 (MQ=255)
                     gcATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGa                                                                    <  2:4207883/93‑1 (MQ=255)
                       aTCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATg                                                                  >  1:2428604/1‑93 (MQ=255)
                       aTCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATg                                                                  >  2:4425520/1‑93 (MQ=255)
                          gggCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCg                                                               >  2:3851185/1‑93 (MQ=255)
                            gCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTg                                                             <  1:753150/93‑1 (MQ=255)
                             cGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGa                                                            >  1:4430225/1‑93 (MQ=255)
                             cGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGa                                                            <  2:2565390/93‑1 (MQ=255)
                              gTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGAc                                                           <  2:1437606/93‑1 (MQ=255)
                              gTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGAc                                                           <  2:3598055/93‑1 (MQ=255)
                               tttAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACt                                                          <  1:4614393/93‑1 (MQ=255)
                               tttAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACt                                                          >  2:222907/1‑93 (MQ=255)
                               tttAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACt                                                          >  2:3230658/1‑93 (MQ=255)
                               tttAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACt                                                          >  2:3287595/1‑93 (MQ=255)
                                ttAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTg                                                         <  2:993605/93‑1 (MQ=255)
                                 tAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGt                                                        >  2:1139224/1‑93 (MQ=255)
                                   gggTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTaa                                                      <  2:362019/93‑1 (MQ=255)
                                    ggTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAAc                                                     <  1:1045430/93‑1 (MQ=255)
                                     gTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAAcc                                                    >  1:3200053/1‑93 (MQ=255)
                                     gTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAAcc                                                    >  2:710799/1‑93 (MQ=255)
                                      tCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGGGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGTCATGACTGTAACCg                                                   >  2:3125127/1‑93 (MQ=255)
                                      tCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCg                                                   >  1:1855898/1‑93 (MQ=255)
                                       cGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGc                                                  <  2:3256047/93‑1 (MQ=255)
                                       cGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCAACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGc                                                  >  1:2632553/1‑93 (MQ=255)
                                         aTCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGctc                                                >  2:416551/1‑93 (MQ=255)
                                         aTCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGctc                                                <  1:3971368/93‑1 (MQ=255)
                                          tCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGGGTGCCTGTAACCGctct                                               >  2:1054498/1‑93 (MQ=255)
                                          tCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGctct                                               >  1:2369253/1‑93 (MQ=255)
                                          tCCCCGGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGctct                                               >  2:2968884/1‑93 (MQ=255)
                                            cccTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCtgt                                             >  1:2011610/1‑93 (MQ=255)
                                             ccTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCtgtt                                            >  1:1452686/1‑93 (MQ=255)
                                              ctgctgCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCtgttg                                           >  1:1174085/1‑93 (MQ=255)
                                               tgctgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCtgttgt                                          <  1:674138/93‑1 (MQ=255)
                                                gctgcCCCAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTTCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTtgtg                                         <  1:2557609/93‑1 (MQ=255)
                                                gctgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTtgtg                                         >  1:325642/1‑93 (MQ=255)
                                                gctgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTtgtg                                         <  2:3327683/93‑1 (MQ=255)
                                                 ctgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTgtgt                                        <  1:928793/93‑1 (MQ=255)
                                                 ctgcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTgtgt                                        >  1:1878202/1‑93 (MQ=255)
                                                   gcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTat                                      >  2:3970460/1‑92 (MQ=255)
                                                   gcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTAc                                      <  2:2396895/93‑1 (MQ=255)
                                                   gcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTAc                                      <  1:4382815/93‑1 (MQ=255)
                                                   gcCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTAc                                      <  1:1266098/93‑1 (MQ=255)
                                                    ccACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACt                                     <  1:2151391/93‑1 (MQ=255)
                                                     cacaATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACtt                                    <  2:95300/93‑1 (MQ=255)
                                                         aTGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCCATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCgg                                >  2:4469242/1‑93 (MQ=255)
                                                         aTGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCgg                                >  1:3713509/1‑93 (MQ=255)
                                                           gAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGgcg                              <  1:228838/93‑1 (MQ=255)
                                                               gcgcCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGgcgagcg                          <  1:631160/93‑1 (MQ=255)
                                                                cgcCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGgcgagcga                         >  2:911607/1‑93 (MQ=255)
                                                                 gcCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGaa                        >  1:1076617/1‑93 (MQ=255)
                                                                 gcCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGaa                        >  1:4224318/1‑93 (MQ=255)
                                                                   cTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAAcc                      <  1:4124769/93‑1 (MQ=255)
                                                                   cTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAAcc                      >  1:3414303/1‑93 (MQ=255)
                                                                   cTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAAcc                      >  1:3986097/1‑93 (MQ=255)
                                                                    tgtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCg                     <  1:4513361/93‑1 (MQ=255)
                                                                    tgtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCg                     >  1:4275815/1‑93 (MQ=255)
                                                                    tgtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCg                     <  1:3913184/93‑1 (MQ=255)
                                                                    tgtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCg                     >  2:3034099/1‑93 (MQ=255)
                                                                    tgtgCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCg                     <  2:3576794/93‑1 (MQ=255)
                                                                      tgctgcGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGt                   <  1:4141141/93‑1 (MQ=255)
                                                                      tgctgcGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGt                   <  1:2985475/93‑1 (MQ=255)
                                                                       gctgcGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTa                  >  2:3607539/1‑93 (MQ=255)
                                                                           cGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTaa              <  2:539327/93‑1 (MQ=255)
                                                                            gTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAAc             <  2:1310698/93‑1 (MQ=255)
                                                                              cAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTACCCTCTCTGTTGTGTACTTTCGGCGAGCGAGCCGGTATTAACca           <  2:1863221/93‑1 (MQ=255)
                                                                              cAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACca           <  1:1508592/93‑1 (MQ=255)
                                                                                aGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACcagc         >  2:4348202/1‑93 (MQ=255)
                                                                                 ggCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACcagca        <  2:804242/93‑1 (MQ=255)
                                                                                     aCCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGAAGGCg    >  2:1657473/1‑93 (MQ=255)
                                                                                       cGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGaa  <  1:3796377/93‑1 (MQ=255)
                                                                                       cGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGaa  >  2:4262568/1‑93 (MQ=255)
                                                                                       cGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGaa  <  1:780577/93‑1 (MQ=255)
                                                                                          |                                                                                         
CATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCTTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAA  >  NC_000913/3679216‑3679395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTCATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCTTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAATTTC  >  NC_000913/3679213‑3679399
                                                                                             |                                                                                             
TGTCATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCC                                                                                               <  SRR4302535.2908627/94‑1 (MQ=60)
 GTCATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCT                                                                                              >  SRR4302535.1897723/1‑94 (MQ=60)
  GCATAAGAGACAATTGCCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTT                                                                                             <  SRR4302535.3215650/94‑1 (MQ=60)
   CATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTG                                                                                            <  SRR4302535.1079735/94‑1 (MQ=60)
    ATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGC                                                                                           >  SRR4302535.581638/1‑94 (MQ=60)
    ATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGC                                                                                           <  SRR4302535.2930269/94‑1 (MQ=60)
    ATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGC                                                                                           >  SRR4302535.4406275/1‑94 (MQ=60)
    ATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGC                                                                                           >  SRR4302535.2475701/1‑94 (MQ=60)
    ATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGC                                                                                           >  SRR4302535.4109371/1‑94 (MQ=60)
     TAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCG                                                                                          >  SRR4302535.2871033/1‑94 (MQ=60)
         AGACAATTACCAGCGGCATCGGTCTTTTAGGATCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAG                                                                                      <  SRR4302535.1966049/94‑1 (MQ=60)
          GACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGC                                                                                     <  SRR4302535.1391483/94‑1 (MQ=60)
           ACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCG                                                                                    >  SRR4302535.7175/1‑94 (MQ=60)
           ACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCG                                                                                    <  SRR4302535.2513537/94‑1 (MQ=60)
           ACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCG                                                                                    <  SRR4302535.3009103/94‑1 (MQ=60)
           ACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCG                                                                                    >  SRR4302535.3155220/1‑94 (MQ=60)
             AATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCA                                                                                  >  SRR4302535.3539311/1‑94 (MQ=60)
             AATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCA                                                                                  >  SRR4302535.1795885/1‑94 (MQ=60)
               TTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAAT                                                                                <  SRR4302535.2097599/94‑1 (MQ=60)
                 TCCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATAC                                                                              <  SRR4302535.4531387/94‑1 (MQ=60)
                   CGGCGGCATCGTGCGCTTAGGGTCCATCCCCTGCTGCCACAATGACCGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGA                                                                            <  SRR4302535.124792/94‑1 (MQ=60)
                   CGGCGGCATCGGGCTTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGA                                                                            <  SRR4302535.2481027/94‑1 (MQ=60)
                   CGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGA                                                                            <  SRR4302535.2000519/94‑1 (MQ=60)
                    GGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAG                                                                           <  SRR4302535.1204584/94‑1 (MQ=60)
                      CGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGG                                                                         <  SRR4302535.569687/94‑1 (MQ=60)
                       GGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGA                                                                        >  SRR4302535.3992086/1‑94 (MQ=60)
                       GGCATCGGGCGTTTAGGGTCGATCCCCTGCTG‑CACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGAT                                                                       <  SRR4302535.3580378/94‑1 (MQ=60)
                        GCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGAT                                                                       <  SRR4302535.3888903/94‑1 (MQ=60)
                        GCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGAT                                                                       <  SRR4302535.1527025/94‑1 (MQ=60)
                        GCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGAT                                                                       <  SRR4302535.169418/94‑1 (MQ=60)
                         CATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATG                                                                      >  SRR4302535.660720/1‑94 (MQ=60)
                         CATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATG                                                                      <  SRR4302535.1937020/94‑1 (MQ=60)
                         CATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATG                                                                      >  SRR4302535.2446103/1‑94 (MQ=60)
                            CTGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCG                                                                   <  SRR4302535.766505/94‑1 (MQ=60)
                             GGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGT                                                                  <  SRR4302535.4009016/94‑1 (MQ=60)
                               GCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGA                                                                <  SRR4302535.758452/94‑1 (MQ=60)
                               GCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGA                                                                <  SRR4302535.2272654/94‑1 (MQ=60)
                               GCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGA                                                                >  SRR4302535.4460580/1‑94 (MQ=60)
                                 GTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACT                                                              <  SRR4302535.1939527/94‑1 (MQ=60)
                                 GTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACT                                                              >  SRR4302535.3566331/1‑94 (MQ=60)
                                  TTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTG                                                             <  SRR4302535.4645744/94‑1 (MQ=60)
                                   TTAGGGTCGAGCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGT                                                            <  SRR4302535.810629/94‑1 (MQ=60)
                                     AGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAA                                                          >  SRR4302535.3634062/1‑94 (MQ=60)
                                      GGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAAC                                                         >  SRR4302535.1151627/1‑94 (MQ=60)
                                       GGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACC                                                        <  SRR4302535.1052838/94‑1 (MQ=60)
                                       GGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACC                                                        >  SRR4302535.3222210/1‑94 (MQ=60)
                                       GGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACC                                                        <  SRR4302535.2698652/94‑1 (MQ=60)
                                        GTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCG                                                       >  SRR4302535.1869266/1‑94 (MQ=60)
                                        GTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCG                                                       <  SRR4302535.2076342/94‑1 (MQ=60)
                                         TCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCAACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGC                                                      >  SRR4302535.2651418/1‑94 (MQ=60)
                                          CGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCT                                                     <  SRR4302535.209917/94‑1 (MQ=60)
                                          CGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCT                                                     <  SRR4302535.4411112/94‑1 (MQ=60)
                                            ATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCT                                                   <  SRR4302535.3998492/94‑1 (MQ=60)
                                            ATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCT                                                   >  SRR4302535.2386262/1‑94 (MQ=60)
                                            ATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCT                                                   <  SRR4302535.270119/94‑1 (MQ=60)
                                            ATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCT                                                   <  SRR4302535.375339/94‑1 (MQ=60)
                                            ATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCT                                                   <  SRR4302535.1458825/94‑1 (MQ=60)
                                             TCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTG                                                  <  SRR4302535.4513438/94‑1 (MQ=60)
                                              CCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGT                                                 >  SRR4302535.2026104/1‑94 (MQ=60)
                                              CCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGT                                                 >  SRR4302535.3428258/1‑94 (MQ=60)
                                              CCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGT                                                 >  SRR4302535.186154/1‑94 (MQ=60)
                                               CCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTT                                                >  SRR4302535.1463082/1‑94 (MQ=60)
                                               CCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTT                                                >  SRR4302535.263033/1‑94 (MQ=60)
                                               CCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTT                                                >  SRR4302535.65061/1‑94 (MQ=60)
                                               CCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTT                                                >  SRR4302535.1876411/1‑94 (MQ=60)
                                                CCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTG                                               >  SRR4302535.1182466/1‑94 (MQ=60)
                                                CCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTG                                               >  SRR4302535.1663573/1‑94 (MQ=60)
                                                  TGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTG                                             <  SRR4302535.678847/94‑1 (MQ=60)
                                                  TGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTG                                             >  SRR4302535.327896/1‑94 (MQ=60)
                                                   GCTGCCCCAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTTCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGT                                            <  SRR4302535.2576023/94‑1 (MQ=60)
                                                   GCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGT                                            >  SRR4302535.1891735/1‑94 (MQ=60)
                                                    CTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTA                                           <  SRR4302535.935367/94‑1 (MQ=60)
                                                     TGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTAC                                          >  SRR4302535.1183120/1‑94 (MQ=60)
                                                     TGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTAC                                          >  SRR4302535.3886774/1‑94 (MQ=60)
                                                      GCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACT                                         <  SRR4302535.4412801/94‑1 (MQ=60)
                                                      GCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACT                                         <  SRR4302535.1275153/94‑1 (MQ=60)
                                                       CCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTT                                        <  SRR4302535.2166856/94‑1 (MQ=60)
                                                       CCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTT                                        <  SRR4302535.4192109/94‑1 (MQ=60)
                                                        CACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTTTTGTGTACTTT                                       <  SRR4302535.2457452/94‑1 (MQ=60)
                                                        CACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTT                                       <  SRR4302535.4243736/94‑1 (MQ=60)
                                                         ACAATGAACGCGCCTGTGCTGCGTCAAGGGCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTC                                      <  SRR4302535.4419137/94‑1 (MQ=60)
                                                         ACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTC                                      <  SRR4302535.2486759/94‑1 (MQ=60)
                                                         ACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTC                                      >  SRR4302535.2523739/1‑94 (MQ=60)
                                                         ACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTC                                      <  SRR4302535.763301/94‑1 (MQ=60)
                                                           AATGATCGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGG                                    <  SRR4302535.4156417/94‑1 (MQ=60)
                                                           AATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGG                                    >  SRR4302535.3739238/1‑94 (MQ=60)
                                                            ATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGC                                   <  SRR4302535.4686819/94‑1 (MQ=60)
                                                              GAACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGA                                 <  SRR4302535.230414/94‑1 (MQ=60)
                                                               AACGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAG                                <  SRR4302535.1794793/94‑1 (MQ=60)
                                                                 CGCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCG                              <  SRR4302535.945218/94‑1 (MQ=60)
                                                                  GCGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGA                             <  SRR4302535.635563/94‑1 (MQ=60)
                                                                   CGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAA                            >  SRR4302535.255142/1‑94 (MQ=60)
                                                                   CGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAA                            >  SRR4302535.1084274/1‑94 (MQ=60)
                                                                   CGCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAA                            >  SRR4302535.4253223/1‑94 (MQ=60)
                                                                    GCCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAAC                           <  SRR4302535.1202283/94‑1 (MQ=60)
                                                                     CCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACC                          >  SRR4302535.4574307/1‑94 (MQ=60)
                                                                     CCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACC                          >  SRR4302535.3438057/1‑94 (MQ=60)
                                                                     CCTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACC                          >  SRR4302535.4013339/1‑94 (MQ=60)
                                                                      CTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCG                         >  SRR4302535.4305083/1‑94 (MQ=60)
                                                                      CTGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCG                         <  SRR4302535.4152997/94‑1 (MQ=60)
                                                                       TGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGG                        >  SRR4302535.2279137/1‑94 (MQ=60)
                                                                       TGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGG                        <  SRR4302535.4544309/94‑1 (MQ=60)
                                                                       TGTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGG                        <  SRR4302535.3939969/94‑1 (MQ=60)
                                                                        GTGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGT                       <  SRR4302535.1508476/94‑1 (MQ=60)
                                                                         TGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTA                      <  SRR4302535.3006414/94‑1 (MQ=60)
                                                                         TGCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTA                      <  SRR4302535.4169498/94‑1 (MQ=60)
                                                                          GCTGCGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTAT                     >  SRR4302535.1851486/1‑94 (MQ=60)
                                                                              CGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAAC                 <  SRR4302535.3752544/94‑1 (MQ=60)
                                                                              CGTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAAC                 <  SRR4302535.3910820/94‑1 (MQ=60)
                                                                               GTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACC                <  SRR4302535.968800/94‑1 (MQ=60)
                                                                               GTCAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACC                >  SRR4302535.3277184/1‑94 (MQ=60)
                                                                                 CAAGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAG              <  SRR4302535.1519436/94‑1 (MQ=60)
                                                                                 CAAGGCCACCGCCTTGCGTTAGCACAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAG              <  SRR4302535.3566007/94‑1 (MQ=60)
                                                                                   AGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCA            <  SRR4302535.4303534/94‑1 (MQ=60)
                                                                                   AGGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCA            >  SRR4302535.3899856/1‑94 (MQ=60)
                                                                                    GGCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAG           <  SRR4302535.3364767/94‑1 (MQ=60)
                                                                                     GCCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGG          <  SRR4302535.2040146/94‑1 (MQ=60)
                                                                                      CCACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGC         >  SRR4302535.2330073/1‑94 (MQ=60)
                                                                                       CACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCG        >  SRR4302535.3478558/1‑94 (MQ=60)
                                                                                       CACCGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCG        >  SRR4302535.4127554/1‑94 (MQ=60)
                                                                                          CGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAAT     <  SRR4302535.3822546/94‑1 (MQ=60)
                                                                                          CGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAAT     <  SRR4302535.786056/94‑1 (MQ=60)
                                                                                          CGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAAT     <  SRR4302535.2394634/94‑1 (MQ=60)
                                                                                          CGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAAT     <  SRR4302535.546190/94‑1 (MQ=60)
                                                                                          CGCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAAACAGCAGGCGAAT     >  SRR4302535.4158489/1‑94 (MQ=60)
                                                                                           GCCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAATT    <  SRR4302535.804643/94‑1 (MQ=60)
                                                                                            CCTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAATTT   <  SRR4302535.3666635/94‑1 (MQ=60)
                                                                                             CTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAATTTC  <  SRR4302535.929358/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGTCATAAGAGACAATTACCGGCGGCATCGGGCGTTTAGGGTCGATCCCCTGCTGCCACAATGAACGCGCCTGTGCTGCGTCAAGGCCACCGCTTTGCGTTAGCGCAATACGAGGGATGGCGTGACTGTAACCGCTCTGTTGTGTACTTTCGGCGAGCGAACCGGTATTAACCAGCAGGCGAATTTC  >  NC_000913/3679213‑3679399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑