Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,702,780 T→C T214A (ACT→GCT)  dppB ← dipeptide transport system permease protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,702,7800TC99.6% 804.8 / ‑5.3 256T214A (ACT→GCT) dppBdipeptide transport system permease protein 1
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (113/141);  total (115/141)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 260 total aligned reads displayed.

CTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGTACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAA  >  NC_000913/3702690‑3702869
                                                                                          |                                                                                         
cTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCAc                                                                                         >  1:1182708/1‑93 (MQ=255)
cTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCAc                                                                                         >  1:1591394/1‑93 (MQ=255)
 tttGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCAcc                                                                                        <  1:1832885/93‑1 (MQ=255)
 tttGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCAcc                                                                                        >  1:487478/1‑93 (MQ=255)
   tGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCa                                                                                      <  1:41987/93‑1 (MQ=255)
    ggCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAg                                                                                     >  1:353073/1‑93 (MQ=255)
    ggCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAg                                                                                     <  2:4192866/93‑1 (MQ=255)
    ggCGCGCGCGGGGCGGATGTAATCCTCGCCCAGCACTTACAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAg                                                                                     >  2:734816/1‑93 (MQ=255)
      cgcgcgcgGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGca                                                                                   <  2:491772/93‑1 (MQ=255)
          cgcgGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAAt                                                                               >  1:3116844/1‑93 (MQ=255)
          cgcgGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAAt                                                                               >  2:1865496/1‑93 (MQ=255)
           gcgGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATg                                                                              <  2:2821548/93‑1 (MQ=255)
           gcgGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATg                                                                              <  2:1915459/93‑1 (MQ=255)
            cgGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATgg                                                                             <  2:2311263/93‑1 (MQ=255)
             ggTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGACTAGCACCCAGCACAATggc                                                                            >  1:1333965/1‑93 (MQ=255)
             ggTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATggc                                                                            <  1:2757081/93‑1 (MQ=255)
               tGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATggcag                                                                          <  2:4149793/93‑1 (MQ=255)
               tGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGAACCCAGCACAATggcag                                                                          >  1:654408/1‑93 (MQ=255)
                 cGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATggcaggc                                                                        <  1:2374355/93‑1 (MQ=255)
                   gATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCaa                                                                      <  1:950706/93‑1 (MQ=255)
                     tGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGa                                                                    <  2:4171350/93‑1 (MQ=255)
                     tGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGa                                                                    <  1:2362421/93‑1 (MQ=255)
                     tGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGa                                                                    <  1:105665/93‑1 (MQ=255)
                        aaTCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCa                                                                 <  2:4552327/93‑1 (MQ=255)
                        aaTCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCa                                                                 <  2:1596162/93‑1 (MQ=255)
                        aaTCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCa                                                                 <  1:2297264/93‑1 (MQ=255)
                          tCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCata                                                               >  2:1955567/1‑93 (MQ=255)
                            cTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATg                                                             >  2:3352335/1‑93 (MQ=255)
                            cTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATg                                                             <  2:2053275/93‑1 (MQ=255)
                            cTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATg                                                             >  2:1003389/1‑93 (MQ=255)
                             tCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATgg                                                            <  1:379090/93‑1 (MQ=255)
                              cGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAACGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATggg                                                           <  1:317655/93‑1 (MQ=255)
                               gCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATgggg                                                          >  2:585043/1‑92 (MQ=255)
                                  cAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGAc                                                       <  2:4630225/93‑1 (MQ=255)
                                  cAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGAc                                                       >  1:3053673/1‑93 (MQ=255)
                                   aGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACg                                                      <  2:240420/93‑1 (MQ=255)
                                   aGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACg                                                      >  1:2623711/1‑93 (MQ=255)
                                    gCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACgg                                                     >  1:2267970/1‑93 (MQ=255)
                                     cACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGc                                                    >  2:954271/1‑93 (MQ=255)
                                     cACTTCAAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGc                                                    >  1:1534386/1‑93 (MQ=255)
                                      aCTTCCAGCATCGAGGAGCGTGTCACACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCa                                                   <  1:1680962/93‑1 (MQ=255)
                                       cTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCAt                                                  <  1:300105/93‑1 (MQ=255)
                                       cTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGAAt                                                  >  1:3877024/1‑93 (MQ=255)
                                        ttCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATc                                                 <  1:4464429/93‑1 (MQ=255)
                                          ccAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGa                                               <  1:3290353/93‑1 (MQ=255)
                                           cAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGAt                                              >  1:4328918/1‑93 (MQ=255)
                                            aGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATa                                             >  1:2175081/1‑93 (MQ=255)
                                            aGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATa                                             <  1:563147/93‑1 (MQ=255)
                                             gCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATaa                                            >  2:2709479/1‑93 (MQ=255)
                                               aTCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAg                                          <  2:468579/93‑1 (MQ=255)
                                               aTCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAg                                          >  2:898511/1‑93 (MQ=255)
                                                tCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGt                                         <  2:3924790/93‑1 (MQ=255)
                                                tCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGt                                         >  1:75229/1‑93 (MQ=255)
                                                tCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGt                                         <  1:2642988/93‑1 (MQ=255)
                                                 cGAGGAGCGTGTCATACGCACAAGGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGtt                                        <  2:1175840/93‑1 (MQ=255)
                                                  gaggagCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTg                                       >  2:1405295/1‑93 (MQ=255)
                                                  gaggagCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTg                                       <  1:3833538/93‑1 (MQ=255)
                                                   aggagCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGc                                      >  2:767438/1‑93 (MQ=255)
                                                   aggagCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGc                                      <  1:1659234/93‑1 (MQ=255)
                                                    ggagCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGcc                                     >  2:3405503/1‑93 (MQ=255)
                                                       gCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTc                                  >  1:3679977/1‑93 (MQ=255)
                                                        cGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCt                                 >  1:1447238/1‑93 (MQ=255)
                                                          tgtCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCtta                               >  1:2905373/1‑92 (MQ=255)
                                                          tgtCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTc                               >  2:3789917/1‑93 (MQ=255)
                                                           gtCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCa                              <  2:1256671/93‑1 (MQ=255)
                                                            tCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAc                             >  1:4262081/1‑93 (MQ=255)
                                                             cATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAcc                            >  1:2871113/1‑93 (MQ=255)
                                                             cATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAcc                            <  1:2045260/93‑1 (MQ=255)
                                                              aTACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAccc                           >  2:3532258/1‑93 (MQ=255)
                                                               tACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAcccc                          <  1:2465225/93‑1 (MQ=255)
                                                               tACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAcccc                          >  2:655502/1‑93 (MQ=255)
                                                               tACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAcccc                          <  1:123829/93‑1 (MQ=255)
                                                               tACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAcccc                          <  2:3479266/93‑1 (MQ=255)
                                                                aCGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCa                         <  2:1699355/93‑1 (MQ=255)
                                                                    acaATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATg                     <  2:256454/93‑1 (MQ=255)
                                                                    acaATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATg                     >  2:320460/1‑93 (MQ=255)
                                                                    acaATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATg                     >  1:8808/1‑93 (MQ=255)
                                                                     caATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATgg                    <  1:2528071/93‑1 (MQ=255)
                                                                       aTGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGGGACGGCATCGATAAAGTTGCCGTCTTGACCCCAGATcgcg                  >  2:2945572/1‑93 (MQ=255)
                                                                          aCCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGgtg               <  1:804841/93‑1 (MQ=255)
                                                                           ccGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGgtgt              <  1:4536477/93‑1 (MQ=255)
                                                                            cGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTc             <  1:2618563/93‑1 (MQ=255)
                                                                            cGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTc             >  2:2783137/1‑93 (MQ=255)
                                                                             gCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCg            >  2:3249145/1‑93 (MQ=255)
                                                                             gCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCg            >  1:1519144/1‑93 (MQ=255)
                                                                             gCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCg            <  1:3259258/93‑1 (MQ=255)
                                                                             gCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCg            >  2:4255668/1‑93 (MQ=255)
                                                                              caaGCGGAATAGCACCCAGCACAATTGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGa           <  2:432967/91‑1 (MQ=255)
                                                                               cAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGAt          >  2:37131/1‑93 (MQ=255)
                                                                                 gCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTa        <  2:1793506/93‑1 (MQ=255)
                                                                                 gCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGAGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTa        >  1:2859789/1‑93 (MQ=255)
                                                                                  cGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAg       <  2:3140600/93‑1 (MQ=255)
                                                                                   ggAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGTCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGc      <  1:4017408/93‑1 (MQ=255)
                                                                                   ggAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGc      >  1:3717782/1‑93 (MQ=255)
                                                                                   ggAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGc      <  2:388841/93‑1 (MQ=255)
                                                                                   ggAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGc      >  1:1125903/1‑93 (MQ=255)
                                                                                    gAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCa     >  1:4553295/1‑93 (MQ=255)
                                                                                      aTAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATa   >  2:4505158/1‑93 (MQ=255)
                                                                                      aTAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATa   <  1:1314138/93‑1 (MQ=255)
                                                                                       tAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGCCTTCACCCCAGATGGTGGTGTCGATTATCATaa  >  2:213792/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGTACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAA  >  NC_000913/3702690‑3702869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGTACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAAAACC  >  NC_000913/3702687‑3702873
                                                                                             |                                                                                             
GCCCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGC                                                                                               <  SRR4302535.2138377/94‑1 (MQ=60)
 CCCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCA                                                                                              <  SRR4302535.767675/94‑1 (MQ=60)
 CCCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCA                                                                                              >  SRR4302535.4061169/1‑94 (MQ=60)
 CCCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCA                                                                                              <  SRR4302535.1241533/94‑1 (MQ=60)
  CCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGGATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCAC                                                                                             >  SRR4302535.2800839/1‑94 (MQ=60)
  CCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCAC                                                                                             >  SRR4302535.1602876/1‑94 (MQ=60)
  CCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCAC                                                                                             <  SRR4302535.2684860/94‑1 (MQ=60)
  CCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCAC                                                                                             >  SRR4302535.1191155/1‑94 (MQ=60)
   CTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACC                                                                                            >  SRR4302535.3949206/1‑94 (MQ=60)
   CTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACC                                                                                            <  SRR4302535.3668950/94‑1 (MQ=60)
   CTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACC                                                                                            >  SRR4302535.490895/1‑94 (MQ=60)
    TTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCC                                                                                           <  SRR4302535.1340943/94‑1 (MQ=60)
    TTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCC                                                                                           <  SRR4302535.1846111/94‑1 (MQ=60)
      TGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAG                                                                                         >  SRR4302535.355509/1‑94 (MQ=60)
      TGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAG                                                                                         <  SRR4302535.42307/94‑1 (MQ=60)
      TGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAG                                                                                         <  SRR4302535.733544/94‑1 (MQ=60)
        GCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCA                                                                                       <  SRR4302535.2960199/94‑1 (MQ=60)
        GCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCA                                                                                       >  SRR4302535.2436593/1‑94 (MQ=60)
        GCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCA                                                                                       <  SRR4302535.1768239/94‑1 (MQ=60)
          GCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACA                                                                                     <  SRR4302535.337073/94‑1 (MQ=60)
           CGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAA                                                                                    >  SRR4302535.4066583/1‑94 (MQ=60)
            GCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAAT                                                                                   >  SRR4302535.3138543/1‑94 (MQ=60)
             CGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATG                                                                                  <  SRR4302535.996983/94‑1 (MQ=60)
              GCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGG                                                                                 <  SRR4302535.3710274/94‑1 (MQ=60)
               CGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGACTAGCACCCAGCACAATGGC                                                                                >  SRR4302535.1343480/1‑94 (MQ=60)
               CGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGC                                                                                <  SRR4302535.1909815/94‑1 (MQ=60)
               CGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGC                                                                                >  SRR4302535.1653940/1‑94 (MQ=60)
                GGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCA                                                                               <  SRR4302535.837392/94‑1 (MQ=60)
                GGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCA                                                                               <  SRR4302535.2776730/94‑1 (MQ=60)
                 GTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAG                                                                              >  SRR4302535.2328203/1‑94 (MQ=60)
                 GTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAG                                                                              <  SRR4302535.2360669/94‑1 (MQ=60)
                 GTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGAACCCAGCACAATGGCAG                                                                              >  SRR4302535.658978/1‑94 (MQ=60)
                    CGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCA                                                                           <  SRR4302535.2391410/94‑1 (MQ=60)
                     GGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGCATAGCACCCAGCACAATCGCAGGCAC                                                                          >  SRR4302535.22124/1‑94 (MQ=60)
                     GGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAA                                                                          >  SRR4302535.2415522/1‑94 (MQ=60)
                      GATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAG                                                                         <  SRR4302535.1329867/94‑1 (MQ=60)
                      GATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAG                                                                         <  SRR4302535.957437/94‑1 (MQ=60)
                      GATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAG                                                                         >  SRR4302535.2541285/1‑94 (MQ=60)
                       ATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGA                                                                        >  SRR4302535.1579638/1‑94 (MQ=60)
                        TGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGAT                                                                       <  SRR4302535.2703219/94‑1 (MQ=60)
                        TGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGAT                                                                       <  SRR4302535.2379388/94‑1 (MQ=60)
                        TGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGAT                                                                       <  SRR4302535.106416/94‑1 (MQ=60)
                          TAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCA                                                                     >  SRR4302535.1262390/1‑94 (MQ=60)
                          TAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCA                                                                     >  SRR4302535.457889/1‑94 (MQ=60)
                          TAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCA                                                                     <  SRR4302535.1207918/94‑1 (MQ=60)
                          TAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCA                                                                     <  SRR4302535.4477643/94‑1 (MQ=60)
                           AATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCAT                                                                    <  SRR4302535.2313704/94‑1 (MQ=60)
                            ATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATA                                                                   <  SRR4302535.4548563/94‑1 (MQ=60)
                             TCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATAT                                                                  <  SRR4302535.2764246/94‑1 (MQ=60)
                             TCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATAT                                                                  >  SRR4302535.2902764/1‑94 (MQ=60)
                             TCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATAT                                                                  <  SRR4302535.1137014/94‑1 (MQ=60)
                              CCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATG                                                                 >  SRR4302535.1494784/1‑94 (MQ=60)
                               CTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGG                                                                <  SRR4302535.2380442/94‑1 (MQ=60)
                                TCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGG                                                               <  SRR4302535.381715/94‑1 (MQ=60)
                                 CGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAACGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGC                                                              <  SRR4302535.319859/94‑1 (MQ=60)
                                  GCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCG                                                             <  SRR4302535.2893936/94‑1 (MQ=60)
                                   CCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGA                                                            >  SRR4302535.380516/1‑94 (MQ=60)
                                    CCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGAC                                                           >  SRR4302535.3075053/1‑94 (MQ=60)
                                     CAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACG                                                          >  SRR4302535.2642513/1‑94 (MQ=60)
                                      AGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGG                                                         >  SRR4302535.2284138/1‑94 (MQ=60)
                                       GCACTTCAAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGC                                                        >  SRR4302535.1545439/1‑94 (MQ=60)
                                         ACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCAT                                                      <  SRR4302535.4246181/94‑1 (MQ=60)
                                         ACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCAT                                                      >  SRR4302535.265913/1‑94 (MQ=60)
                                         ACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCAT                                                      <  SRR4302535.2882582/94‑1 (MQ=60)
                                         ACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGAAT                                                      >  SRR4302535.3903650/1‑94 (MQ=60)
                                         ACTTCCAGCATCGAGGAGCGTGTCACACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCAT                                                      <  SRR4302535.1693137/94‑1 (MQ=60)
                                          CTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATC                                                     <  SRR4302535.654886/94‑1 (MQ=60)
                                          CTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATC                                                     <  SRR4302535.302196/94‑1 (MQ=60)
                                           TTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCG                                                    <  SRR4302535.4495050/94‑1 (MQ=60)
                                           TTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCG                                                    <  SRR4302535.3816110/94‑1 (MQ=60)
                                             CCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGAT                                                  >  SRR4302535.4358517/1‑94 (MQ=60)
                                             CCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGAT                                                  <  SRR4302535.3313229/94‑1 (MQ=60)
                                              CAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATA                                                 >  SRR4302535.2190633/1‑94 (MQ=60)
                                              CAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATA                                                 <  SRR4302535.3884707/94‑1 (MQ=60)
                                              CAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATA                                                 >  SRR4302535.2190634/1‑94 (MQ=60)
                                               AGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAA                                                <  SRR4302535.776162/94‑1 (MQ=60)
                                               AGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAA                                                <  SRR4302535.567091/94‑1 (MQ=60)
                                                 CATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAG                                              <  SRR4302535.3076531/94‑1 (MQ=60)
                                                  ATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGT                                             >  SRR4302535.75779/1‑94 (MQ=60)
                                                  ATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGT                                             <  SRR4302535.260423/94‑1 (MQ=60)
                                                  ATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGT                                             >  SRR4302535.4685622/1‑94 (MQ=60)
                                                   TCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTT                                            <  SRR4302535.2661919/94‑1 (MQ=60)
                                                   TCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTT                                            <  SRR4302535.214012/94‑1 (MQ=60)
                                                     GAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGC                                          >  SRR4302535.2151544/1‑94 (MQ=60)
                                                     GAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGC                                          <  SRR4302535.3859974/94‑1 (MQ=60)
                                                      AGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCC                                         <  SRR4302535.1671229/94‑1 (MQ=60)
                                                      AGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCC                                         >  SRR4302535.3053214/1‑94 (MQ=60)
                                                       GGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCG                                        <  SRR4302535.3047317/94‑1 (MQ=60)
                                                         AGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTC                                      >  SRR4302535.3705445/1‑94 (MQ=60)
                                                         AGCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTC                                      >  SRR4302535.3267474/1‑94 (MQ=60)
                                                          GCGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCT                                     >  SRR4302535.1457587/1‑94 (MQ=60)
                                                           CGTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTT                                    >  SRR4302535.4335704/1‑94 (MQ=60)
                                                            GTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTC                                   <  SRR4302535.1592938/94‑1 (MQ=60)
                                                            GTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTC                                   <  SRR4302535.165169/94‑1 (MQ=60)
                                                            GTGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTA                                   >  SRR4302535.2925910/1‑94 (MQ=60)
                                                             TGTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCA                                  <  SRR4302535.3257492/94‑1 (MQ=60)
                                                              GTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAC                                 <  SRR4302535.154257/94‑1 (MQ=60)
                                                              GTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAC                                 >  SRR4302535.4291271/1‑94 (MQ=60)
                                                              GTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAC                                 >  SRR4302535.4482493/1‑94 (MQ=60)
                                                              GTCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCAC                                 <  SRR4302535.2640265/94‑1 (MQ=60)
                                                               TCATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACC                                >  SRR4302535.2891455/1‑94 (MQ=60)
                                                                CATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCC                               <  SRR4302535.2059991/94‑1 (MQ=60)
                                                                 ATACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCC                              >  SRR4302535.3688548/1‑94 (MQ=60)
                                                                  TACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCA                             <  SRR4302535.2483001/94‑1 (MQ=60)
                                                                  TACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCA                             <  SRR4302535.124697/94‑1 (MQ=60)
                                                                   ACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAG                            <  SRR4302535.2638130/94‑1 (MQ=60)
                                                                   ACGCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAG                            <  SRR4302535.2305815/94‑1 (MQ=60)
                                                                     GCACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGAT                          <  SRR4302535.4553197/94‑1 (MQ=60)
                                                                      CACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATG                         >  SRR4302535.8880/1‑94 (MQ=60)
                                                                      CACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATG                         <  SRR4302535.4041016/94‑1 (MQ=60)
                                                                      CACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATG                         >  SRR4302535.67200/1‑94 (MQ=60)
                                                                      CACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATG                         <  SRR4302535.2504579/94‑1 (MQ=60)
                                                                      CACAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATG                         <  SRR4302535.2791753/94‑1 (MQ=60)
                                                                        CAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGC                       <  SRR4302535.566832/94‑1 (MQ=60)
                                                                        CAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGC                       >  SRR4302535.608435/1‑94 (MQ=60)
                                                                        CAATGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGC                       <  SRR4302535.2546282/94‑1 (MQ=60)
                                                                         AATGACCGCCAGCGGAATAGCACCCCGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCG                      >  SRR4302535.1430398/1‑94 (MQ=60)
                                                                           TGACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGT                    <  SRR4302535.1092829/94‑1 (MQ=60)
                                                                            GACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTG                   <  SRR4302535.3111827/94‑1 (MQ=60)
                                                                             ACCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGT                  <  SRR4302535.810492/94‑1 (MQ=60)
                                                                              CCGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTC                 <  SRR4302535.4567514/94‑1 (MQ=60)
                                                                               CGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCG                <  SRR4302535.2637336/94‑1 (MQ=60)
                                                                               CGCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCG                >  SRR4302535.1530085/1‑94 (MQ=60)
                                                                               CGCCAGCGGAATAGCACCCAGCAAAACGGCAACGAAGATCATATGCGCGAAGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGCTGTCG                <  SRR4302535.3329862/94‑1 (MQ=53)
                                                                                GCCAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGA               <  SRR4302535.3281911/94‑1 (MQ=60)
                                                                                  CAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATT             >  SRR4302535.4655532/1‑94 (MQ=60)
                                                                                  CAGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATT             >  SRR4302535.152594/1‑94 (MQ=60)
                                                                                   AGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTA            >  SRR4302535.1279926/1‑94 (MQ=60)
                                                                                   AGCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGAGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTA            >  SRR4302535.2880046/1‑94 (MQ=60)
                                                                                    GCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAG           <  SRR4302535.3877985/94‑1 (MQ=60)
                                                                                    GCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAG           <  SRR4302535.979545/94‑1 (MQ=60)
                                                                                    GCGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAG           >  SRR4302535.4242519/1‑94 (MQ=60)
                                                                                     CGTAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGC          <  SRR4302535.901381/94‑1 (MQ=60)
                                                                                     CGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGC          >  SRR4302535.1672234/1‑94 (MQ=60)
                                                                                     CGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGC          >  SRR4302535.1133940/1‑94 (MQ=60)
                                                                                     CGGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGC          >  SRR4302535.3743543/1‑94 (MQ=60)
                                                                                      GGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGTCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCA         <  SRR4302535.4044888/94‑1 (MQ=60)
                                                                                      GGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCA         >  SRR4302535.4584406/1‑94 (MQ=60)
                                                                                      GGAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCA         >  SRR4302535.1842930/1‑94 (MQ=60)
                                                                                       GAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCAT        <  SRR4302535.3302711/94‑1 (MQ=60)
                                                                                       GAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCAT        <  SRR4302535.3848531/94‑1 (MQ=60)
                                                                                       GAATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGGCTTCACTCCAGATGGCGGTGTCGATTAGCAT        >  SRR4302535.1174871/1‑94 (MQ=60)
                                                                                        AATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATA       <  SRR4302535.318518/94‑1 (MQ=60)
                                                                                         ATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAA      <  SRR4302535.821774/94‑1 (MQ=60)
                                                                                         ATAGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAA      <  SRR4302535.1323530/94‑1 (MQ=60)
                                                                                           AGCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAAAA    <  SRR4302535.4549150/94‑1 (MQ=60)
                                                                                           AGCACCCAGCACAATGGCAGGCAAGATCATATGCGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAAAA    <  SRR4302535.4429585/94‑1 (MQ=60)
                                                                                            GCACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAAAAC   <  SRR4302535.2575744/94‑1 (MQ=60)
                                                                                             CACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAAAACC  <  SRR4302535.4676911/94‑1 (MQ=60)
                                                                                             CACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAAAACC  <  SRR4302535.3947057/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCCCTTTGGCGCGCGCGGTGCGGATGTAATCCTCGCCCAGCACTTCCAGCATCGAGGAGCGTGTCATACGCACAATGACCGCCAGCGGAATAGTACCCAGCACAATGGCAGGCAAGATCATATGGGCGACGGCATCGATAAAGTTGCCGTCTTCACCCCAGATGGCGGTGTCGATTAGCATAAAACC  >  NC_000913/3702687‑3702873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑