Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,706,692	T→C	E482E (GAA→GAG)	yhjW ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,706,692	0	T	C	97.5%	852.8 / 6.7	282	E482E (GAA→GAG)	yhjW	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (3/4); new base C (126/149); total (129/153)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 292 total aligned reads displayed.

TCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGTTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATC  >  NC_000913/3706600‑3706781

   aGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCa                                                                                        <  2:3497345/93‑1 (MQ=255)

   aGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCa                                                                                        <  1:1920618/93‑1 (MQ=255)

   aGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCa                                                                                        <  2:3905242/93‑1 (MQ=255)

    gATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCAt                                                                                       >  1:3071941/1‑93 (MQ=255)

       atTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTaa                                                                                    >  1:224517/1‑93 (MQ=255)

       atTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTaa                                                                                    >  1:1871398/1‑93 (MQ=255)

        tttATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAAt                                                                                   <  1:31546/93‑1 (MQ=255)

        tttATCTGACATCCAGACCATCATCGGCACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCAGTAAt                                                                                   >  1:4681593/1‑93 (MQ=255)

         ttATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAAtt                                                                                  <  1:1179870/93‑1 (MQ=255)

          tATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTg                                                                                 >  1:4581841/1‑93 (MQ=255)

           aTCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGa                                                                                >  1:4518905/1‑93 (MQ=255)

           aTCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGa                                                                                <  2:1532465/93‑1 (MQ=255)

             cTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACt                                                                              <  1:3594150/93‑1 (MQ=255)

              tGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTc                                                                             <  1:3130236/93‑1 (MQ=255)

              tGACATCCAGACCATCATCGGTACGCGGAACTGCT‑CGGCGGTGCCAGTTCACGCGGCGTTCCGTGCAGGTGCTCGAGCTCATTAATTGACTCa                                                                            >  1:2905033/1‑93 (MQ=255)

               taCATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCa                                                                            <  2:3097369/92‑1 (MQ=255)

               gACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCa                                                                            <  1:2812978/93‑1 (MQ=255)

                aCATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCAc                                                                           <  2:2180000/93‑1 (MQ=255)

                   tCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACGGt                                                                        >  1:2692680/1‑93 (MQ=255)

                   tCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGt                                                                        >  1:2773162/1‑93 (MQ=255)

                   tCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGt                                                                        <  1:3769891/93‑1 (MQ=255)

                   tCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGt                                                                        <  1:808416/93‑1 (MQ=255)

                    ccAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTg                                                                       >  1:4488785/1‑93 (MQ=255)

                     cAGCCCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTgg                                                                      <  1:2525731/93‑1 (MQ=255)

                     cAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTgg                                                                      >  2:940648/1‑93 (MQ=255)

                      aGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGt                                                                     >  2:2067169/1‑93 (MQ=255)

                      aGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGt                                                                     >  2:2770869/1‑93 (MQ=255)

                      aGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGt                                                                     <  1:839058/93‑1 (MQ=255)

                       gACCATCATCGGTACGCGGGACTGCTCCGGCGGTGGCAGGTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTgggt                                                                    >  2:4344250/1‑91 (MQ=255)

                       gACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTc                                                                    >  2:4649562/1‑93 (MQ=255)

                       gACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTACACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCCTGGTc                                                                    >  2:3827920/1‑93 (MQ=255)

                        aCCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCa                                                                   >  1:4442533/1‑93 (MQ=255)

                         ctatcatcGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAg                                                                  >  1:3520760/3‑93 (MQ=255)

                          catcatCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGTTCATTAATTGACTCACCGTGGTCAGc                                                                 <  2:895474/93‑1 (MQ=255)

                          catcatCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGc                                                                 >  2:2709138/1‑93 (MQ=255)

                            tcatcGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTg                                                               >  2:234824/1‑93 (MQ=255)

                            tcatcGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTg                                                               >  2:2635308/1‑93 (MQ=255)

                            tcatcGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTg                                                               <  2:867064/93‑1 (MQ=255)

                            tcatcGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTg                                                               >  1:1057781/1‑93 (MQ=255)

                             catcGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGc                                                              <  1:4380198/93‑1 (MQ=255)

                              atcGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCg                                                             >  2:3819108/1‑93 (MQ=255)

                               tcGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGt                                                            >  2:3284042/1‑93 (MQ=255)

                                   tACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGaa                                                        <  2:2310374/93‑1 (MQ=255)

                                   tACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGaa                                                        >  1:476674/1‑93 (MQ=255)

                                    aCGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAac                                                       <  1:323047/93‑1 (MQ=255)

                                     cgcgGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAaca                                                      >  2:3608814/1‑93 (MQ=255)

                                       cgGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGTTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAAcaca                                                    >  2:3246221/1‑93 (MQ=255)

                                        ggAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACaa                                                   >  2:2555948/1‑93 (MQ=255)

                                          aaCTGCTCCGGCGGTGCCAGTTCACGCGTCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATc                                                 <  2:3629875/93‑1 (MQ=255)

                                           aCTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCg                                                <  2:3145911/93‑1 (MQ=255)

                                           aCTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCg                                                >  2:1774891/1‑93 (MQ=255)

                                              gCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCtt                                             <  1:3305356/93‑1 (MQ=255)

                                                tCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTc                                           <  2:1142558/93‑1 (MQ=255)

                                                 ccGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCt                                          <  1:1418134/93‑1 (MQ=255)

                                                   ggcggTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTa                                        <  2:2979615/93‑1 (MQ=255)

                                                   ggcggTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTa                                        <  1:3375016/93‑1 (MQ=255)

                                                    gcggTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTAt                                       >  2:3713735/1‑93 (MQ=255)

                                                    gcggTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTAt                                       <  2:1940221/93‑1 (MQ=255)

                                                       gTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATcgc                                    <  2:1838862/93‑1 (MQ=255)

                                                       gTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATcgc                                    >  1:349209/1‑93 (MQ=255)

                                                         gCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGa                                  <  2:4658659/93‑1 (MQ=255)

                                                          ccAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGaa                                 >  1:1897799/1‑93 (MQ=255)

                                                          ccAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGaa                                 <  2:287180/93‑1 (MQ=255)

                                                             gTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCt                              <  1:1028033/93‑1 (MQ=255)

                                                             gTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCt                              >  2:3444515/1‑93 (MQ=255)

                                                             gTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCt                              <  2:4169995/93‑1 (MQ=255)

                                                             gTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCt                              <  2:566292/93‑1 (MQ=255)

                                                             gTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCt                              <  2:2600361/93‑1 (MQ=255)

                                                              ttCACGCCGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTg                             <  1:3664531/93‑1 (MQ=255)

                                                               tCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGa                            <  1:247883/93‑1 (MQ=255)

                                                                cACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGat                           <  1:427671/93‑1 (MQ=255)

                                                                cACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGat                           <  2:3327677/93‑1 (MQ=255)

                                                                 aCGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGatc                          <  2:3989046/93‑1 (MQ=255)

                                                                  cgcgGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGatca                         <  2:1124882/93‑1 (MQ=255)

                                                                   gcggcgTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGatcaa                        >  1:2405708/1‑93 (MQ=255)

                                                                   gcggcgTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGatcaa                        <  2:3358660/93‑1 (MQ=255)

                                                                   gcggcgTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGatcaa                        <  1:4005479/93‑1 (MQ=255)

                                                                     ggcgTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGatcaatc                      <  1:1829681/93‑1 (MQ=255)

                                                                      gcgTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGatcaatca                     <  1:3780671/93‑1 (MQ=255)

                                                                      gcgTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGatcaatca                     >  1:3800260/1‑93 (MQ=255)

                                                                      gcgTGCCGGGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGTACCTGatcaatca                     >  2:4119502/1‑93 (MQ=255)

                                                                       cgtgccgtgcAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCAc                    <  1:2379812/93‑1 (MQ=255)

                                                                        gtgccgtgcAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACg                   >  2:2415524/1‑93 (MQ=255)

                                                                          gccgtgcAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCt                 <  1:3916665/93‑1 (MQ=255)

                                                                             gtgcAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGga              >  2:4476069/1‑93 (MQ=255)

                                                                             gtgcAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGga              <  2:1028284/93‑1 (MQ=255)

                                                                               gcAGGTGCTCGCGTTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGgaga            <  2:4537372/93‑1 (MQ=255)

                                                                               gcAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGgaga            <  2:4087122/93‑1 (MQ=255)

                                                                               gcAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGgaga            <  1:3847982/93‑1 (MQ=255)

                                                                                 aGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATa          <  2:141184/93‑1 (MQ=255)

                                                                                  ggTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATaa         <  2:683186/93‑1 (MQ=255)

                                                                                  ggTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATaa         >  1:1389525/1‑93 (MQ=255)

                                                                                   gTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATaaa        <  2:1007084/93‑1 (MQ=255)

                                                                                    tGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAg       <  1:2054935/93‑1 (MQ=255)

                                                                                      cTCGCGTTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTg     <  1:1550920/93‑1 (MQ=255)

                                                                                      cTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTg     >  1:662291/1‑93 (MQ=255)

                                                                                      cTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTg     <  2:2788013/93‑1 (MQ=255)

                                                                                        cgcgCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGAt   <  1:3043669/93‑1 (MQ=255)

                                                                                         gcgcTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATc  >  2:4246951/1‑93 (MQ=255)

                                                                                         gcgcTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATc  >  2:1232881/1‑93 (MQ=255)

TCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGTTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATC  >  NC_000913/3706600‑3706781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGTTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCCACA  >  NC_000913/3706600‑3706785

TCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGTT                                                                                              <  SRR4302535.3666158/94‑1 (MQ=60)

TCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCT                                                                                              >  SRR4302535.2660128/1‑94 (MQ=60)

TCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCT                                                                                              >  SRR4302535.3570022/1‑94 (MQ=60)

  CAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCA                                                                                            >  SRR4302535.2383701/1‑94 (MQ=60)

   AGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCAT                                                                                           >  SRR4302535.3093388/1‑94 (MQ=60)

   AGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCAT                                                                                           <  SRR4302535.1934463/94‑1 (MQ=60)

     ATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTA                                                                                         >  SRR4302535.2481190/1‑94 (MQ=60)

      TATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAA                                                                                        >  SRR4302535.226064/1‑94 (MQ=60)

      TATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAA                                                                                        <  SRR4302535.3139549/94‑1 (MQ=60)

      TATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAA                                                                                        >  SRR4302535.1884885/1‑94 (MQ=60)

       ATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAAT                                                                                       >  SRR4302535.1777940/1‑94 (MQ=60)

       ATTTATCTGACATCCAGACCATCATCGGCACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCAGTAAT                                                                                       >  SRR4302535.4713228/1‑94 (MQ=60)

        TTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATT                                                                                      <  SRR4302535.31787/94‑1 (MQ=60)

         TTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTG                                                                                     <  SRR4302535.1188289/94‑1 (MQ=60)

         TTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTG                                                                                     >  SRR4302535.4613043/1‑94 (MQ=60)

          TATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGA                                                                                    >  SRR4302535.4549879/1‑94 (MQ=60)

           ATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGAC                                                                                   >  SRR4302535.47247/1‑94 (MQ=60)

            TCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACT                                                                                  >  SRR4302535.1909509/1‑94 (MQ=60)

             CTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTC                                                                                 <  SRR4302535.3619092/94‑1 (MQ=60)

             CTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTC                                                                                 >  SRR4302535.2625509/1‑94 (MQ=60)

             CTGACATCCAGACCATCATCGGTACGCGGAACTGCT‑CGGCGGTGCCAGTTCACGCGGCGTTCCGTGCAGGTGCTCGAGCTCATTAATTGACTCA                                                                                >  SRR4302535.2925568/1‑94 (MQ=60)

              TGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCA                                                                                <  SRR4302535.3152037/94‑1 (MQ=60)

              TGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCA                                                                                <  SRR4302535.15247/94‑1 (MQ=60)

               GACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCAC                                                                               <  SRR4302535.2832968/94‑1 (MQ=60)

                ACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACC                                                                              <  SRR4302535.3069940/94‑1 (MQ=60)

                 CATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCG                                                                             >  SRR4302535.1872970/1‑94 (MQ=60)

                  ATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACGGT                                                                            >  SRR4302535.2711927/1‑94 (MQ=60)

                  ATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGT                                                                            >  SRR4302535.2917877/1‑94 (MQ=60)

                  ATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGT                                                                            >  SRR4302535.2711928/1‑94 (MQ=60)

                  ATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGT                                                                            >  SRR4302535.3906924/1‑94 (MQ=60)

                  ATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGT                                                                            <  SRR4302535.4492883/94‑1 (MQ=60)

                  ATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGT                                                                            >  SRR4302535.2792908/1‑94 (MQ=60)

                   TCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTG                                                                           <  SRR4302535.3795909/94‑1 (MQ=60)

                   TCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTG                                                                           >  SRR4302535.4519599/1‑94 (MQ=60)

                   TCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTG                                                                           <  SRR4302535.1983012/94‑1 (MQ=60)

                   TCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTG                                                                           <  SRR4302535.1106470/94‑1 (MQ=60)

                   TCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTG                                                                           <  SRR4302535.814076/94‑1 (MQ=60)

                     CAGCCCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGT                                                                         <  SRR4302535.2543930/94‑1 (MQ=60)

                      AGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTC                                                                        <  SRR4302535.844950/94‑1 (MQ=60)

                       GACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCA                                                                       >  SRR4302535.4472998/1‑94 (MQ=60)

                        ACTATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAG                                                                      >  SRR4302535.3545212/1‑94 (MQ=60)

                         CCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGC                                                                     >  SRR4302535.3126040/1‑94 (MQ=60)

                         CCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGC                                                                     >  SRR4302535.3855430/1‑94 (MQ=60)

                          CATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCT                                                                    >  SRR4302535.1935290/1‑94 (MQ=60)

                          CATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCT                                                                    <  SRR4302535.3212218/94‑1 (MQ=60)

                          CATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCT                                                                    <  SRR4302535.2952550/94‑1 (MQ=60)

                           ATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTG                                                                   >  SRR4302535.1065303/1‑94 (MQ=60)

                           ATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTG                                                                   >  SRR4302535.2817302/1‑94 (MQ=60)

                           ATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTG                                                                   >  SRR4302535.331131/1‑94 (MQ=60)

                             CATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCG                                                                 <  SRR4302535.3353579/94‑1 (MQ=60)

                             CATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCG                                                                 >  SRR4302535.3545170/1‑94 (MQ=60)

                             CATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCG                                                                 >  SRR4302535.1403155/1‑94 (MQ=60)

                             CATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCG                                                                 <  SRR4302535.4410167/94‑1 (MQ=60)

                             CATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCG                                                                 >  SRR4302535.3574752/1‑94 (MQ=60)

                              ATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGTTCATTAATTGACTCACCGTGGTCAGCTGCGT                                                                >  SRR4302535.4464002/1‑94 (MQ=60)

                              ATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGT                                                                >  SRR4302535.3519241/1‑94 (MQ=60)

                                CGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAG                                                              <  SRR4302535.2770135/94‑1 (MQ=60)

                                CGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAG                                                              >  SRR4302535.3152795/1‑94 (MQ=60)

                                CGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAG                                                              >  SRR4302535.4164531/1‑94 (MQ=60)

                                 GGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGGCAGCTGCGTAGA                                                             >  SRR4302535.4668945/1‑94 (MQ=60)

                                  GTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAA                                                            >  SRR4302535.480019/1‑94 (MQ=60)

                                   TACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAAC                                                           <  SRR4302535.3079867/94‑1 (MQ=60)

                                    ACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACA                                                          <  SRR4302535.325281/94‑1 (MQ=60)

                                      GCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACA                                                        <  SRR4302535.484381/94‑1 (MQ=60)

                                        GGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAAT                                                      <  SRR4302535.3421554/94‑1 (MQ=60)

                                         GAACTGCTCCGGCGGTGCCAGTTCACGCGTCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGCCTCACCGTGGTCAGCTGCGTAGAACACAATC                                                     <  SRR4302535.3762134/94‑1 (MQ=60)

                                         GAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATC                                                     <  SRR4302535.856949/94‑1 (MQ=60)

                                          AACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACGCACCGTGGTCAGCTGCGTAGAACACAATCG                                                    <  SRR4302535.1793690/94‑1 (MQ=60)

                                           ACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGC                                                   <  SRR4302535.4707419/94‑1 (MQ=60)

                                            CTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCT                                                  >  SRR4302535.809876/1‑94 (MQ=60)

                                            CTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCT                                                  <  SRR4302535.3934878/94‑1 (MQ=60)

                                              GCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTT                                                <  SRR4302535.3328352/94‑1 (MQ=60)

                                                TCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCT                                              <  SRR4302535.3798320/94‑1 (MQ=60)

                                                TCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCT                                              >  SRR4302535.2797353/1‑94 (MQ=60)

                                                TCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCT                                              <  SRR4302535.3797575/94‑1 (MQ=60)

                                                 CCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTT                                             <  SRR4302535.1428259/94‑1 (MQ=60)

                                                 CCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTT                                             <  SRR4302535.1283014/94‑1 (MQ=60)

                                                  CGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTA                                            >  SRR4302535.456600/1‑94 (MQ=60)

                                                   GGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTAT                                           <  SRR4302535.1907320/94‑1 (MQ=60)

                                                   GGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTAT                                           <  SRR4302535.3398489/94‑1 (MQ=60)

                                                     CGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCG                                         <  SRR4302535.4536085/94‑1 (MQ=60)

                                                      GGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGC                                        >  SRR4302535.1918058/1‑94 (MQ=60)

                                                      GGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGC                                        >  SRR4302535.2477531/1‑94 (MQ=60)

                                                      GGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGC                                        >  SRR4302535.351613/1‑94 (MQ=60)

                                                       GTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCG                                       >  SRR4302535.4481751/1‑94 (MQ=60)

                                                       GTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCG                                       <  SRR4302535.1198387/94‑1 (MQ=60)

                                                        TGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGA                                      >  SRR4302535.3482019/1‑94 (MQ=60)

                                                         GCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAA                                     >  SRR4302535.915588/1‑94 (MQ=60)

                                                         GCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAA                                     >  SRR4302535.1911451/1‑94 (MQ=60)

                                                           CAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACC                                   >  SRR4302535.1864001/1‑94 (MQ=60)

                                                           CAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACC                                   >  SRR4302535.2113070/1‑94 (MQ=60)

                                                           CAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACC                                   <  SRR4302535.2906563/94‑1 (MQ=60)

                                                           CAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACC                                   >  SRR4302535.3868588/1‑94 (MQ=60)

                                                            AGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCT                                  >  SRR4302535.480943/1‑94 (MQ=60)

                                                             GTTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTG                                 <  SRR4302535.1035333/94‑1 (MQ=60)

                                                              TTCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGA                                <  SRR4302535.2518461/94‑1 (MQ=60)

                                                              TTCACGCCGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGA                                <  SRR4302535.3689905/94‑1 (MQ=60)

                                                               TCACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGAT                               <  SRR4302535.249586/94‑1 (MQ=60)

                                                                CACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATC                              >  SRR4302535.47252/1‑94 (MQ=60)

                                                                CACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATC                              <  SRR4302535.430644/94‑1 (MQ=60)

                                                                 ACGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCA                             <  SRR4302535.4460678/94‑1 (MQ=60)

                                                                  CGCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAA                            >  SRR4302535.2423024/1‑94 (MQ=60)

                                                                   GCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAAT                           <  SRR4302535.2420994/94‑1 (MQ=60)

                                                                   GCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAAT                           <  SRR4302535.3817886/94‑1 (MQ=60)

                                                                   GCGGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAAT                           <  SRR4302535.4032879/94‑1 (MQ=60)

                                                                     GGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCA                         >  SRR4302535.3826456/1‑94 (MQ=60)

                                                                     GGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCA                         >  SRR4302535.2391316/1‑94 (MQ=60)

                                                                     GGCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCA                         <  SRR4302535.1842892/94‑1 (MQ=60)

                                                                      GCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCAC                        <  SRR4302535.3806763/94‑1 (MQ=60)

                                                                      GCGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCAC                        <  SRR4302535.830055/94‑1 (MQ=60)

                                                                       CGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACG                       >  SRR4302535.3170446/1‑94 (MQ=60)

                                                                       CGTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACG                       <  SRR4302535.2396900/94‑1 (MQ=60)

                                                                        GTGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGC                      >  SRR4302535.4563430/1‑94 (MQ=60)

                                                                         TGCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCT                     <  SRR4302535.2561929/94‑1 (MQ=60)

                                                                          GCCGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTG                    <  SRR4302535.3943466/94‑1 (MQ=60)

                                                                            CGTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGA                  >  SRR4302535.4180206/1‑94 (MQ=60)

                                                                             GTGCAGGTGCTCGCGTTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAG                 >  SRR4302535.1455177/1‑94 (MQ=60)

                                                                             GTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAG                 >  SRR4302535.1917825/1‑94 (MQ=60)

                                                                             GTGCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAG                 <  SRR4302535.4520048/94‑1 (MQ=60)

                                                                               GCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGAT               <  SRR4302535.3298063/94‑1 (MQ=60)

                                                                               GCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGAT               <  SRR4302535.3874468/94‑1 (MQ=60)

                                                                               GCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGAT               <  SRR4302535.2533297/94‑1 (MQ=60)

                                                                               GCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGAT               <  SRR4302535.1047358/94‑1 (MQ=60)

                                                                               GCAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGAT               >  SRR4302535.2966892/1‑94 (MQ=60)

                                                                                CAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATA              <  SRR4302535.3646275/94‑1 (MQ=60)

                                                                                CAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATA              <  SRR4302535.77558/94‑1 (MQ=60)

                                                                                CAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATA              >  SRR4302535.844054/1‑94 (MQ=60)

                                                                                CAGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATA              <  SRR4302535.3750846/94‑1 (MQ=60)

                                                                                 AGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAA             <  SRR4302535.3180562/94‑1 (MQ=60)

                                                                                 AGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAA             >  SRR4302535.1399442/1‑94 (MQ=60)

                                                                                 AGGTGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAA             >  SRR4302535.4316977/1‑94 (MQ=60)

                                                                                    TGCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGT          <  SRR4302535.2069736/94‑1 (MQ=60)

                                                                                     GCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTG         <  SRR4302535.2198482/94‑1 (MQ=60)

                                                                                     GCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTG         >  SRR4302535.3809535/1‑94 (MQ=60)

                                                                                     GCTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTG         >  SRR4302535.666914/1‑94 (MQ=60)

                                                                                      CTCGCGTTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGA        <  SRR4302535.1562090/94‑1 (MQ=60)

                                                                                      CTCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGA        >  SRR4302535.3984614/1‑94 (MQ=60)

                                                                                       TCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGAT       <  SRR4302535.3597514/94‑1 (MQ=60)

                                                                                       TCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGAT       <  SRR4302535.1417589/94‑1 (MQ=60)

                                                                                       TCGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGAT       <  SRR4302535.4013269/94‑1 (MQ=60)

                                                                                        CGCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATC      <  SRR4302535.3064992/94‑1 (MQ=60)

                                                                                         GCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCC     >  SRR4302535.2217904/1‑94 (MQ=60)

                                                                                         GCGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCC     <  SRR4302535.1147465/94‑1 (MQ=60)

                                                                                          CGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCCA    <  SRR4302535.152835/94‑1 (MQ=60)

                                                                                          CGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCCA    >  SRR4302535.616707/1‑94 (MQ=60)

                                                                                          CGCTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCCA    <  SRR4302535.3133131/94‑1 (MQ=60)

                                                                                            CTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCCACA  <  SRR4302535.1084077/94‑1 (MQ=60)

                                                                                            CTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCCACA  <  SRR4302535.4174314/94‑1 (MQ=60)

                                                                                            CTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCCACA  <  SRR4302535.3255474/94‑1 (MQ=60)

TCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGCTCGCGTTCATTAATTGACTCACCGTGGTCAGCTGCGTAGAACACAATCGCTTTCTTATCGCGAACCTGATCAATCACGCTGGAGATAAAGTGATCCACA  >  NC_000913/3706600‑3706785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`