Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,190	(C)_6→5	coding (948/1725 nt)	yhjW ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,185	0	C	.	99.6%	1062.9 / ‑3.0	234	coding (953/1725 nt)	yhjW	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (132/101); minor base T (0/1); total (132/102)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

GCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTG  >  NC_000913/3707093‑3707273

gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcgc                                                                                          >  1:2130239/1‑93 (MQ=255)

  aTAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcgcac                                                                                        >  2:1454932/1‑91 (MQ=255)

  aTAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccc                                                                                        >  2:2064558/1‑93 (MQ=255)

   tAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccc                                                                                       <  2:75262/93‑1 (MQ=255)

   tAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGcccc                                                                                       >  2:3735562/1‑93 (MQ=255)

     gCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccct                                                                                     >  2:4646490/1‑92 (MQ=255)

       gTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccctga                                                                                   >  2:25535/1‑90 (MQ=255)

       gTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGccccctga                                                                                   <  2:3493762/93‑4 (MQ=255)

          gagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGt                                                                               >  1:4630472/1‑93 (MQ=255)

            ggTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTAc                                                                             >  1:930824/1‑93 (MQ=255)

            ggTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTAc                                                                             >  2:1128447/1‑93 (MQ=255)

             gTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACa                                                                            >  1:2698582/1‑93 (MQ=255)

                 gAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACa                                                                        <  1:621483/93‑1 (MQ=255)

                 gAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACa                                                                        <  1:2438607/93‑1 (MQ=255)

                 gAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACa                                                                        <  2:1426358/93‑1 (MQ=255)

                 gAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACa                                                                        >  1:2882658/1‑93 (MQ=255)

                  aaCTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACAt                                                                       >  1:1805457/1‑93 (MQ=255)

                   aCTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATg                                                                      >  2:3896776/1‑93 (MQ=255)

                      gAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCaa                                                                   <  1:431757/93‑1 (MQ=255)

                      gAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCaa                                                                   <  1:1982353/93‑1 (MQ=255)

                       aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAAc                                                                  <  1:289427/93‑1 (MQ=255)

                       aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAAc                                                                  <  2:1001963/93‑1 (MQ=255)

                       aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAAc                                                                  <  1:2654216/93‑1 (MQ=255)

                         tCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGAAACGc                                                                >  2:198627/1‑93 (MQ=255)

                          ccTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCa                                                               >  1:2358188/1‑93 (MQ=255)

                           cTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAg                                                              <  1:2287805/93‑1 (MQ=255)

                            tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGt                                                             <  1:1404140/93‑1 (MQ=255)

                            tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGt                                                             >  2:444746/1‑93 (MQ=255)

                             aaCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTg                                                            >  1:2529007/1‑93 (MQ=255)

                             aaCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGAGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTg                                                            >  1:3299454/1‑93 (MQ=255)

                              aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTga                                                           >  2:2400345/1‑93 (MQ=255)

                              aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTga                                                           <  2:2625846/93‑1 (MQ=255)

                              aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGAGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTga                                                           >  1:3384388/1‑93 (MQ=255)

                                tGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTgaga                                                         <  2:1253112/93‑1 (MQ=255)

                                tGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTgaga                                                         >  1:25931/1‑93 (MQ=255)

                                tGCTTCAGAACCGCGAAAATATTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTgaga                                                         <  1:4386715/93‑1 (MQ=255)

                                   ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGtt                                                      >  1:342947/1‑93 (MQ=255)

                                   ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGtt                                                      <  1:4296071/93‑1 (MQ=255)

                                     cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTg                                                    <  2:2001852/93‑1 (MQ=255)

                                      aGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTgg                                                   >  1:413789/1‑93 (MQ=255)

                                       gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                  <  1:566754/93‑1 (MQ=255)

                                       gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                  <  2:2841488/93‑1 (MQ=255)

                                       gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                  <  1:838355/93‑1 (MQ=255)

                                       gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                  >  2:4380195/1‑93 (MQ=255)

                                       gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                  <  2:840566/93‑1 (MQ=255)

                                       gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                  >  1:4379744/1‑93 (MQ=255)

                                          ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                               <  1:38486/93‑1 (MQ=255)

                                          ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                               >  1:160357/1‑93 (MQ=255)

                                            gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                             >  1:3317329/1‑93 (MQ=255)

                                            gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                             >  1:3664802/1‑93 (MQ=255)

                                              gAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTa                                           <  2:4279712/93‑1 (MQ=255)

                                              gAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTa                                           <  1:3056551/93‑1 (MQ=255)

                                              gAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTa                                           <  1:2012478/93‑1 (MQ=255)

                                               aaaaTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTAt                                          <  1:4567457/93‑1 (MQ=255)

                                                aaaTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATc                                         <  2:703232/93‑1 (MQ=255)

                                                aaaTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATc                                         <  1:1224764/93‑1 (MQ=255)

                                                 aaTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                        >  2:3273706/1‑93 (MQ=255)

                                                  aTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                       >  1:4261586/1‑93 (MQ=255)

                                                  aTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                       >  1:2798473/1‑93 (MQ=255)

                                                  aTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                       >  1:683727/1‑93 (MQ=255)

                                                  aTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                       <  1:448680/93‑1 (MQ=255)

                                                   tgttctgttcTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcaca                                      <  1:3119057/93‑1 (MQ=255)

                                                   tgttctgttcTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcaca                                      <  2:4142922/93‑1 (MQ=255)

                                                       ctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg                                  <  2:1803304/93‑1 (MQ=255)

                                                        tgttctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                 >  1:3705914/1‑93 (MQ=255)

                                                         gttctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTa                                >  2:3192132/1‑93 (MQ=255)

                                                         gttctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTa                                >  2:2022202/1‑93 (MQ=255)

                                                          ttctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCCCAGGAGTaa                               <  2:3044134/93‑1 (MQ=255)

                                                          ttctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa                               >  2:2605494/1‑93 (MQ=255)

                                                           tctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAc                              <  2:2973448/93‑1 (MQ=255)

                                                            ctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                             >  2:3638707/1‑93 (MQ=255)

                                                            ctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                             >  1:3598831/1‑93 (MQ=255)

                                                             ttttAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCa                            >  2:2145669/1‑93 (MQ=255)

                                                             ttttAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCa                            >  2:2363925/1‑93 (MQ=255)

                                                             ttttAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGGTTGGTTGCGGTATCACAGGAGTAACCa                            >  2:383047/1‑93 (MQ=255)

                                                              tttAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCAc                           <  2:725638/93‑1 (MQ=255)

                                                                 aaTGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGa                        >  2:2686250/1‑93 (MQ=255)

                                                                 aaTGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGa                        <  1:130392/93‑1 (MQ=255)

                                                                 aaTGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGa                        <  2:2274980/93‑1 (MQ=255)

                                                                  aTGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGaa                       >  1:3462603/1‑93 (MQ=255)

                                                                  aTGTGCGCTGCGGATTATCTTCAGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGaa                       >  2:660221/1‑93 (MQ=255)

                                                                   tgtgCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc                      <  1:3811997/93‑1 (MQ=255)

                                                                        gcTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcgccgc                 <  2:2855237/93‑1 (MQ=255)

                                                                         cTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACgccgcc                >  2:3938682/1‑93 (MQ=255)

                                                                         cTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACgccgcc                <  2:532150/93‑1 (MQ=255)

                                                                          tGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa               >  1:213645/1‑93 (MQ=255)

                                                                          tGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa               >  1:878379/1‑93 (MQ=255)

                                                                           gCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAg              >  2:1634425/1‑93 (MQ=255)

                                                                           gCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAg              >  1:103441/1‑93 (MQ=255)

                                                                              gATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAtt           >  1:2339497/1‑93 (MQ=255)

                                                                              gATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAtt           <  2:3166794/93‑1 (MQ=255)

                                                                              gATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAtt           >  2:594303/1‑93 (MQ=255)

                                                                                  aTCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAA‑CACGGAACGCCGCCAGATTTTtct      <  2:3181456/93‑1 (MQ=255)

                                                                                   tCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtct      >  1:3549928/1‑93 (MQ=255)

                                                                                   tCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtct      <  1:3901330/93‑1 (MQ=255)

                                                                                     ttCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc    <  2:4053857/93‑1 (MQ=255)

                                                                                     ttCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTcc    >  1:3208550/1‑93 (MQ=255)

                                                                                      tCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCt   >  1:40458/1‑93 (MQ=255)

                                                                                      tCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCt   >  2:4252206/1‑93 (MQ=255)

                                                                                      tCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCt   <  1:454838/93‑1 (MQ=255)

GCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTG  >  NC_000913/3707093‑3707273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTC  >  NC_000913/3707097‑3707283

AGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCT                                                                                               <  SRR4302535.231162/94‑1 (MQ=60)

AGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCT                                                                                               <  SRR4302535.4485831/94‑1 (MQ=60)

AGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCT                                                                                               >  SRR4302535.2422850/1‑94 (MQ=60)

  CGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGA                                                                                            >  SRR4302535.690290/1‑94 (MQ=60)

  CGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGA                                                                                            >  SRR4302535.3337532/1‑94 (MQ=60)

   GTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGAC                                                                                           <  SRR4302535.2785704/94‑1 (MQ=60)

     AGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGT                                                                                         >  SRR4302535.4661890/1‑94 (MQ=60)

       AGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTAC                                                                                       >  SRR4302535.937405/1‑94 (MQ=60)

        GGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACA                                                                                      >  SRR4302535.2717867/1‑94 (MQ=60)

         GTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAA                                                                                     >  SRR4302535.3899523/1‑94 (MQ=60)

           CAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAAC                                                                                   >  SRR4302535.4415314/1‑94 (MQ=60)

            AGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACA                                                                                  >  SRR4302535.2903060/1‑94 (MQ=60)

            AGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACA                                                                                  <  SRR4302535.3473478/94‑1 (MQ=60)

             GAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACAT                                                                                 <  SRR4302535.3293665/94‑1 (MQ=60)

             GAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACAT                                                                                 >  SRR4302535.1818503/1‑94 (MQ=60)

             GAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACAT                                                                                 <  SRR4302535.2456174/94‑1 (MQ=60)

             GAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACAT                                                                                 <  SRR4302535.625798/94‑1 (MQ=60)

             GAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACAT                                                                                 <  SRR4302535.2394896/94‑1 (MQ=60)

               ACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGC                                                                               <  SRR4302535.3914701/94‑1 (MQ=60)

               ACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGC                                                                               >  SRR4302535.1233734/1‑94 (MQ=60)

                CTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCA                                                                              >  SRR4302535.2235721/1‑94 (MQ=60)

                CTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCA                                                                              >  SRR4302535.3608822/1‑94 (MQ=60)

                CTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCA                                                                              >  SRR4302535.3420645/1‑94 (MQ=60)

                  GAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAAC                                                                            <  SRR4302535.434764/94‑1 (MQ=60)

                  GAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAAC                                                                            <  SRR4302535.1996626/94‑1 (MQ=60)

                  GAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAAC                                                                            <  SRR4302535.2362952/94‑1 (MQ=60)

                   AATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACG                                                                           <  SRR4302535.2673232/94‑1 (MQ=60)

                   AATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACG                                                                           <  SRR4302535.291452/94‑1 (MQ=60)

                   AATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACG                                                                           >  SRR4302535.3683928/1‑94 (MQ=60)

                   AATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACG                                                                           >  SRR4302535.2569012/1‑94 (MQ=60)

                    ATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGC                                                                          <  SRR4302535.3545713/94‑1 (MQ=60)

                     TCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCA                                                                         >  SRR4302535.2375105/1‑94 (MQ=60)

                     TCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCA                                                                         <  SRR4302535.2795485/94‑1 (MQ=60)

                      CCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAG                                                                        >  SRR4302535.3428040/1‑94 (MQ=60)

                       CTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGT                                                                       <  SRR4302535.2304146/94‑1 (MQ=60)

                        TAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTG                                                                      >  SRR4302535.4591698/1‑94 (MQ=60)

                        TAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTG                                                                      >  SRR4302535.2547229/1‑94 (MQ=60)

                        TAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTG                                                                      <  SRR4302535.1414156/94‑1 (MQ=60)

                        TAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGAGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTG                                                                      >  SRR4302535.3322400/1‑94 (MQ=60)

                         AACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGA                                                                     >  SRR4302535.3695626/1‑94 (MQ=60)

                         AACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGA                                                                     >  SRR4302535.880983/1‑94 (MQ=60)

                         AACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGAGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGA                                                                     >  SRR4302535.3407936/1‑94 (MQ=60)

                          ACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAG                                                                    <  SRR4302535.915522/94‑1 (MQ=60)

                          ACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAG                                                                    <  SRR4302535.3857399/94‑1 (MQ=60)

                          ACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAG                                                                    >  SRR4302535.902397/1‑94 (MQ=60)

                           CTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGA                                                                   >  SRR4302535.26132/1‑94 (MQ=60)

                            TGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAG                                                                  >  SRR4302535.4226865/1‑94 (MQ=60)

                            TGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAG                                                                  <  SRR4302535.3051902/94‑1 (MQ=60)

                            TGCTTCAGAACCGCGAAAATATTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAG                                                                  <  SRR4302535.4416725/94‑1 (MQ=60)

                              CTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTT                                                                >  SRR4302535.4347098/1‑94 (MQ=60)

                              CTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTT                                                                >  SRR4302535.345319/1‑94 (MQ=60)

                               TTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTT                                                               <  SRR4302535.4325468/94‑1 (MQ=60)

                               TTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTT                                                               >  SRR4302535.1512163/1‑94 (MQ=60)

                                 CAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGG                                                             <  SRR4302535.2583540/94‑1 (MQ=60)

                                 CAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGG                                                             >  SRR4302535.416694/1‑94 (MQ=60)

                                  AGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGT                                                            >  SRR4302535.4409710/1‑94 (MQ=60)

                                  AGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGT                                                            <  SRR4302535.3740066/94‑1 (MQ=60)

                                  AGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGCGAGAGTTTGGT                                                            >  SRR4302535.758495/1‑94 (MQ=60)

                                   GAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTT                                                           >  SRR4302535.4547562/1‑94 (MQ=60)

                                   GAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTT                                                           <  SRR4302535.1966468/94‑1 (MQ=60)

                                   GAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTT                                                           <  SRR4302535.4296356/94‑1 (MQ=60)

                                   GAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTT                                                           <  SRR4302535.570722/94‑1 (MQ=60)

                                   GAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTT                                                           <  SRR4302535.844242/94‑1 (MQ=60)

                                    AACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTG                                                          <  SRR4302535.2716100/94‑1 (MQ=60)

                                     ACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGC                                                         >  SRR4302535.161482/1‑94 (MQ=60)

                                     ACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGC                                                         >  SRR4302535.3953181/1‑94 (MQ=60)

                                     ACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGC                                                         <  SRR4302535.1450324/94‑1 (MQ=60)

                                      CCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCG                                                        <  SRR4302535.38782/94‑1 (MQ=60)

                                      CCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCG                                                        >  SRR4302535.3295338/1‑94 (MQ=60)

                                       CGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGG                                                       >  SRR4302535.440213/1‑94 (MQ=60)

                                       CGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGG                                                       <  SRR4302535.2351110/94‑1 (MQ=60)

                                       CGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGG                                                       >  SRR4302535.3340410/1‑94 (MQ=60)

                                       CGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGG                                                       >  SRR4302535.3690180/1‑94 (MQ=60)

                                         CGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTA                                                     >  SRR4302535.2096533/1‑94 (MQ=60)

                                          GAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTAT                                                    >  SRR4302535.2154287/1‑94 (MQ=60)

                                          GAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTAT                                                    <  SRR4302535.2026975/94‑1 (MQ=60)

                                          GAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTAT                                                    <  SRR4302535.3077943/94‑1 (MQ=60)

                                           AAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATC                                                   <  SRR4302535.3339133/94‑1 (MQ=60)

                                           AAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATC                                                   <  SRR4302535.4598611/94‑1 (MQ=60)

                                           AAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATC                                                   >  SRR4302535.1726651/1‑94 (MQ=60)

                                           AAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATC                                                   >  SRR4302535.2864467/1‑94 (MQ=60)

                                            AAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCA                                                  <  SRR4302535.1233523/94‑1 (MQ=60)

                                            AAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCA                                                  >  SRR4302535.4624949/1‑94 (MQ=60)

                                             AATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCAC                                                 >  SRR4302535.3579547/1‑94 (MQ=60)

                                             AATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCAC                                                 >  SRR4302535.688512/1‑94 (MQ=60)

                                             AATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCAC                                                 >  SRR4302535.4290774/1‑94 (MQ=60)

                                             AATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCAC                                                 >  SRR4302535.2818388/1‑94 (MQ=60)

                                             AATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCAC                                                 >  SRR4302535.4609546/1‑94 (MQ=60)

                                              ATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACA                                                <  SRR4302535.451824/94‑1 (MQ=60)

                                               TGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAG                                               <  SRR4302535.3140770/94‑1 (MQ=60)

                                               TGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAG                                               >  SRR4302535.584369/1‑94 (MQ=60)

                                                GTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGG                                              >  SRR4302535.3098172/1‑94 (MQ=60)

                                                  TCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAG                                            >  SRR4302535.3814636/1‑94 (MQ=60)

                                                  TCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAG                                            <  SRR4302535.4184559/94‑1 (MQ=60)

                                                  TCTGTTCTTTTAATGCGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAG                                            <  SRR4302535.1132772/94‑1 (MQ=60)

                                                   CTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGT                                           <  SRR4302535.2150602/94‑1 (MQ=60)

                                                   CTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGT                                           <  SRR4302535.2651699/94‑1 (MQ=60)

                                                   CTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGT                                           >  SRR4302535.3731577/1‑94 (MQ=60)

                                                      TTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAC                                        <  SRR4302535.4468182/94‑1 (MQ=60)

                                                       TCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACC                                       >  SRR4302535.4365378/1‑94 (MQ=60)

                                                       TCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACC                                       >  SRR4302535.3623805/1‑94 (MQ=60)

                                                          TTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACG                                    >  SRR4302535.2627727/1‑94 (MQ=60)

                                                            TAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGA                                  >  SRR4302535.9851/1‑94 (MQ=60)

                                                             AATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAA                                 >  SRR4302535.2826868/1‑94 (MQ=60)

                                                             AATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAA                                 >  SRR4302535.3486672/1‑94 (MQ=60)

                                                             AATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAA                                 <  SRR4302535.131308/94‑1 (MQ=60)

                                                              ATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAC                                <  SRR4302535.2003767/94‑1 (MQ=60)

                                                               TGTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACG                               <  SRR4302535.3838300/94‑1 (MQ=60)

                                                                GTGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGC                              >  SRR4302535.565196/1‑94 (MQ=60)

                                                                 TGCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCC                             >  SRR4302535.4528855/1‑94 (MQ=60)

                                                                  GCGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCG                            <  SRR4302535.3013566/94‑1 (MQ=60)

                                                                   CGCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCCCGGAACGCCGC                           >  SRR4302535.169230/1‑94 (MQ=60)

                                                                    GCTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCC                          >  SRR4302535.4067356/1‑94 (MQ=60)

                                                                     CTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCA                         >  SRR4302535.215109/1‑94 (MQ=60)

                                                                     CTGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCA                         >  SRR4302535.884598/1‑94 (MQ=60)

                                                                      TGCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAG                        >  SRR4302535.104174/1‑94 (MQ=60)

                                                                       GCGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA                       <  SRR4302535.1317860/94‑1 (MQ=60)

                                                                        CGGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAT                      <  SRR4302535.3173508/94‑1 (MQ=60)

                                                                         GGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATT                     >  SRR4302535.2356235/1‑94 (MQ=60)

                                                                         GGATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATT                     >  SRR4302535.2911806/1‑94 (MQ=60)

                                                                          GATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTT                    >  SRR4302535.68514/1‑94 (MQ=60)

                                                                          GATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTT                    <  SRR4302535.4098398/94‑1 (MQ=60)

                                                                           ATTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTT                   >  SRR4302535.2082806/1‑94 (MQ=60)

                                                                            TTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTT                  >  SRR4302535.3081956/1‑94 (MQ=60)

                                                                            TTATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTT                  <  SRR4302535.3561326/94‑1 (MQ=60)

                                                                              ATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCT                <  SRR4302535.4326495/94‑1 (MQ=60)

                                                                              ATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCT                <  SRR4302535.662038/94‑1 (MQ=60)

                                                                              ATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCT                >  SRR4302535.2535381/1‑94 (MQ=60)

                                                                              ATCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCT                >  SRR4302535.3574565/1‑94 (MQ=60)

                                                                               TCTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTC               <  SRR4302535.3928053/94‑1 (MQ=60)

                                                                                CTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCC              >  SRR4302535.3230773/1‑94 (MQ=60)

                                                                                CTTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCC              >  SRR4302535.1284409/1‑94 (MQ=60)

                                                                                 TTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCT             >  SRR4302535.40765/1‑94 (MQ=60)

                                                                                 TTCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCT             <  SRR4302535.3276062/94‑1 (MQ=60)

                                                                                  TCCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTG            <  SRR4302535.458023/94‑1 (MQ=60)

                                                                                   CCGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGG           <  SRR4302535.491569/94‑1 (MQ=60)

                                                                                    CGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGG          <  SRR4302535.1079428/94‑1 (MQ=60)

                                                                                    CGCG‑CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGG          >  SRR4302535.4450481/1‑94 (MQ=60)

                                                                                       cgCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCC        >  SRR4302535.4205436/3‑94 (MQ=60)

                                                                                       cgCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCC        >  SRR4302535.3465734/3‑94 (MQ=60)

                                                                                        GCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCA       >  SRR4302535.2478771/1‑94 (MQ=60)

                                                                                         CCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAG      >  SRR4302535.2203963/1‑94 (MQ=60)

                                                                                          CCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGCACGCCGCCAGATTTTTCTCCTGGGCCAGT     >  SRR4302535.987601/1‑94 (MQ=60)

                                                                                            CCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTT   >  SRR4302535.4277930/1‑94 (MQ=60)

                                                                                             CTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTC  <  SRR4302535.2477412/94‑1 (MQ=60)

AGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTC  >  NC_000913/3707097‑3707283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`