Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,744,796 G→A G28D (GGC→GAC)  lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,744,7960GA99.5% 641.8 / ‑5.4 187G28D (GGC→GAC) lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (88/97);  total (89/98)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01

Only 100 of 211 total aligned reads displayed.

AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA  >  NC_000913/3744706‑3744888
                                                                                          |                                                                                            
aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACg                                                                                            >  2:2584143/1‑93 (MQ=255)
 aTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACgg                                                                                           >  2:3904168/1‑92 (MQ=255)
  tAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTg                                                                                          >  1:2191101/1‑93 (MQ=255)
  tAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGGAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCCGACGTg                                                                                          >  2:2924961/1‑93 (MQ=255)
    aaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGcg                                                                                        >  2:3503483/1‑92 (MQ=255)
     aCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAg                                                                                       <  1:1598797/93‑1 (MQ=255)
      ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgc                                                                                      >  2:1384176/1‑93 (MQ=255)
       cATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgcg                                                                                     >  1:862403/1‑93 (MQ=255)
        aTGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgcgc                                                                                    >  1:1023121/1‑93 (MQ=255)
        aTGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAgcgc                                                                                    <  2:2018585/93‑1 (MQ=255)
         tGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGcc                                                                                   <  1:4478419/93‑1 (MQ=255)
            cGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGc                                                                                <  2:3979923/93‑1 (MQ=255)
            cGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGc                                                                                >  2:4269643/1‑93 (MQ=255)
            cGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGc                                                                                >  2:2939861/1‑93 (MQ=255)
            cGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGc                                                                                >  1:2691228/1‑93 (MQ=255)
             gCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGcc                                                                               >  2:1790644/1‑93 (MQ=255)
               aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGc                                                                             >  1:2569393/1‑93 (MQ=255)
               aaTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGc                                                                             <  1:2890097/93‑1 (MQ=255)
                aTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCt                                                                            >  1:2984137/1‑93 (MQ=255)
                aTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCt                                                                            >  2:290784/1‑93 (MQ=255)
                aTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCt                                                                            >  2:4172213/1‑93 (MQ=255)
                aTACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGACGCt                                                                            >  1:397626/1‑93 (MQ=255)
                 tACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtg                                                                           >  2:21292/1‑93 (MQ=255)
                  aCTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGCAGGCCGGGAGGCAGACGTGCAGCGCCCGCCGCtgg                                                                          >  1:1309296/1‑92 (MQ=255)
                  aCTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtgt                                                                          <  1:1753991/93‑1 (MQ=255)
                  aCTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtgt                                                                          <  2:30223/93‑1 (MQ=255)
                  aCTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtgt                                                                          <  1:2849680/93‑1 (MQ=255)
                  aCTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTAAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCtgt                                                                          <  1:327570/93‑1 (MQ=255)
                   ctggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGGGCCTGCCGCtgtg                                                                         >  2:3838483/1‑93 (MQ=255)
                    tggctggTGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgc                                                                        <  1:1546420/93‑1 (MQ=255)
                     ggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcg                                                                       >  1:4627456/1‑93 (MQ=255)
                     ggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcg                                                                       >  1:248675/1‑93 (MQ=255)
                     ggctggGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTgcg                                                                       <  1:594472/93‑1 (MQ=255)
                         ggggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAtt                                                                   <  1:919781/93‑1 (MQ=255)
                          gggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTa                                                                  <  1:3138322/93‑1 (MQ=255)
                            gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                <  1:4556446/93‑1 (MQ=255)
                            gTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                >  1:4179429/1‑93 (MQ=255)
                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                              >  1:2320015/1‑93 (MQ=255)
                              tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGcc                                                              <  2:2448981/93‑1 (MQ=255)
                               aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGccc                                                             <  2:1027934/93‑1 (MQ=255)
                                gATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCg                                                            >  1:1125324/1‑93 (MQ=255)
                                 aTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGAAGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGc                                                           >  2:4322643/1‑93 (MQ=255)
                                 aTTGTGGCGGTAGCTGGCTAAAAGCAGGGCTGTATGACCGCGAAGGCCCGGAGGCAGACGCGCAGGGCCTGGCGCTGTGCGCATTAAGCCCGc                                                           >  2:4575194/1‑93 (MQ=255)
                                  ttGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCa                                                          >  2:4663757/1‑93 (MQ=255)
                                  ttGTGGCGGTAGCTGGCTGAAACCCGGGCTGTATGACCGCGAAGGCCGGGCGGCAGACGTGCAGTGCCTGCCGCCGTGCGCATTAAGCCCGCa                                                          >  2:3812457/1‑93 (MQ=255)
                                   tgtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAg                                                         <  2:2034826/93‑1 (MQ=255)
                                     tgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGcc                                                       >  1:2766750/1‑93 (MQ=255)
                                      ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                      <  2:3209575/93‑1 (MQ=255)
                                      ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCa                                                      >  1:3023891/1‑93 (MQ=255)
                                       gCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAg                                                     <  2:1627624/93‑1 (MQ=255)
                                             gctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTggg                                               >  1:307270/1‑93 (MQ=255)
                                             gctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTggg                                               <  1:4156695/93‑1 (MQ=255)
                                             gctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTggg                                               <  1:1673402/93‑1 (MQ=255)
                                             gctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTggg                                               >  1:1909309/1‑93 (MQ=255)
                                              ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                              <  2:2987371/93‑1 (MQ=255)
                                              ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                              <  2:824865/93‑1 (MQ=255)
                                              ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGc                                              <  1:4290101/93‑1 (MQ=255)
                                               tggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCa                                             <  2:2557304/93‑1 (MQ=255)
                                                 gctgAAAGCCGGGCTTAATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                           <  1:4393860/93‑1 (MQ=255)
                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                           >  1:1413239/1‑93 (MQ=255)
                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                           <  2:15908/93‑1 (MQ=255)
                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                           >  2:2209236/1‑93 (MQ=255)
                                                 gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCaga                                           <  2:3857830/93‑1 (MQ=255)
                                                  ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCagag                                          <  1:374349/93‑1 (MQ=255)
                                                   tgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgc                                         <  1:2054508/93‑1 (MQ=255)
                                                    gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                        <  1:3549865/93‑1 (MQ=255)
                                                    gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                        >  1:2436029/1‑93 (MQ=255)
                                                      aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGcgcg                                      <  1:3932428/93‑1 (MQ=255)
                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  1:3175808/93‑1 (MQ=255)
                                                         ccGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                   <  1:2736040/93‑1 (MQ=255)
                                                          cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGatat                                  <  2:2669304/93‑1 (MQ=255)
                                                            ggCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATgg                                >  1:814314/1‑93 (MQ=255)
                                                             gCTGTAGGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGAGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGc                               <  2:3003270/93‑1 (MQ=255)
                                                              cTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCa                              >  2:2515838/1‑93 (MQ=255)
                                                              cTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCa                              >  2:1755979/1‑93 (MQ=255)
                                                               tGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAg                             >  1:468330/1‑93 (MQ=255)
                                                               tGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAg                             >  1:419723/1‑93 (MQ=255)
                                                                 tATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGGCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGAGATGGCAGaa                           >  2:2319078/1‑93 (MQ=255)
                                                                 tATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAgga                           >  2:884899/1‑91 (MQ=255)
                                                                 tATCACCGAGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGGGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGgaagaa                           <  2:1236062/93‑1 (MQ=255)
                                                                  aTGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAc                          <  2:2119800/93‑1 (MQ=255)
                                                                    gACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACtg                        <  2:133005/93‑1 (MQ=255)
                                                                       cgcgAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTgg                     <  1:2932544/93‑1 (MQ=255)
                                                                        gcgAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGCGAGAGCGCGATAGCGCAGAACTGTggg                    >  2:2407057/1‑92 (MQ=255)
                                                                          gAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCaa                  <  1:2511110/93‑1 (MQ=255)
                                                                          gAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCaa                  <  2:789155/93‑1 (MQ=255)
                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCGAt                 >  2:3723178/1‑93 (MQ=255)
                                                                           aaGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAt                 <  2:3222764/93‑1 (MQ=255)
                                                                            aGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGGAAtg                >  2:3591396/1‑93 (MQ=255)
                                                                            aGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtg                >  2:1697834/1‑93 (MQ=255)
                                                                             ggCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgc               >  2:669269/1‑93 (MQ=255)
                                                                             ggCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgc               <  1:4107373/93‑1 (MQ=255)
                                                                              gCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGCACCGTGGCCAtccc              >  2:2200612/1‑90 (MQ=255)
                                                                               ccGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgctg             <  2:4505386/93‑1 (MQ=255)
                                                                                cGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgctgc            <  2:2993702/93‑1 (MQ=255)
                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          <  1:1836610/93‑1 (MQ=255)
                                                                                  ggAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt          <  1:2177844/93‑1 (MQ=255)
                                                                                    aggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg        <  1:4346607/93‑1 (MQ=255)
                                                                                     ggcagacGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       >  1:2611771/1‑93 (MQ=255)
                                                                                     ggcagacGTGCAGCCCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc       <  1:670954/93‑1 (MQ=255)
                                                                                          |                                                                                            
AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA  >  NC_000913/3744706‑3744888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  >  NC_000913/3744704‑3744889
                                                                                            |                                                                                             
GCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGAC                                                                                              >  SRR4302535.2033876/1‑94 (MQ=60)
 CAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCCGTATGACCGCGAAGGCCGGGAGGCAGACG                                                                                             >  SRR4302535.2135088/1‑94 (MQ=60)
 CAAAAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACG                                                                                             <  SRR4302535.3374668/94‑1 (MQ=60)
  AATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGT                                                                                            >  SRR4302535.4197245/1‑94 (MQ=60)
   ATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTG                                                                                           >  SRR4302535.2206697/1‑94 (MQ=60)
     AAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCA                                                                                         <  SRR4302535.3164724/94‑1 (MQ=60)
     AAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCA                                                                                         <  SRR4302535.3259772/94‑1 (MQ=60)
       ACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGC                                                                                       <  SRR4302535.1610327/94‑1 (MQ=60)
       ACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGC                                                                                       <  SRR4302535.1944834/94‑1 (MQ=60)
        CCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCG                                                                                      >  SRR4302535.868502/1‑94 (MQ=60)
        CCATGACGCAATACTGGCTGGGGGTAGATTGTGGCGGGAGCTGGCTGAAACACCGGCTGTATGACCGCGAACGCCGGGAGGCAGACGTGCAGCC                                                                                      >  SRR4302535.3634061/1‑94 (MQ=60)
         CATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGC                                                                                     >  SRR4302535.1030389/1‑94 (MQ=60)
           TGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCT                                                                                   <  SRR4302535.4509166/94‑1 (MQ=60)
             ACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGC                                                                                 <  SRR4302535.4364316/94‑1 (MQ=60)
             ACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGC                                                                                 >  SRR4302535.2710464/1‑94 (MQ=60)
             ACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGC                                                                                 >  SRR4302535.3396439/1‑94 (MQ=60)
              CGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCC                                                                                <  SRR4302535.4508747/94‑1 (MQ=60)
                CAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGC                                                                              >  SRR4302535.2587872/1‑94 (MQ=60)
                 AATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCT                                                                             >  SRR4302535.3005070/1‑94 (MQ=60)
                 AATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCT                                                                             <  SRR4302535.2910543/94‑1 (MQ=60)
                 AATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGACGCT                                                                             >  SRR4302535.400413/1‑94 (MQ=60)
                   TACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGCAGGCCGGGAGGCAGACGTGCAGCGCCCGCCGCTGG                                                                           >  SRR4302535.1318653/1‑94 (MQ=60)
                    ACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTG                                                                          <  SRR4302535.2869870/94‑1 (MQ=60)
                    ACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTG                                                                          <  SRR4302535.1766667/94‑1 (MQ=60)
                    ACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTAAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTG                                                                          <  SRR4302535.329841/94‑1 (MQ=60)
                     CTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGC                                                                         <  SRR4302535.2584475/94‑1 (MQ=60)
                     CTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGC                                                                         <  SRR4302535.4250885/94‑1 (MQ=60)
                      TGGCTGGTGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCG                                                                        <  SRR4302535.1557559/94‑1 (MQ=60)
                      TGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCG                                                                        >  SRR4302535.250385/1‑94 (MQ=60)
                      TGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCG                                                                        <  SRR4302535.2511486/94‑1 (MQ=60)
                      TGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCG                                                                        >  SRR4302535.4658868/1‑94 (MQ=60)
                       GGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGC                                                                       <  SRR4302535.598640/94‑1 (MQ=60)
                        GCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCA                                                                      >  SRR4302535.4406447/1‑94 (MQ=60)
                         CTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCAT                                                                     >  SRR4302535.28363/1‑94 (MQ=60)
                           GGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTA                                                                   <  SRR4302535.926304/94‑1 (MQ=60)
                           GGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCCGCCGCTGTGCGCATTA                                                                   >  SRR4302535.989812/1‑94 (MQ=60)
                            GGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAA                                                                  <  SRR4302535.3160161/94‑1 (MQ=60)
                            GGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAA                                                                  >  SRR4302535.2205075/1‑94 (MQ=60)
                             GGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAG                                                                 >  SRR4302535.4208034/1‑94 (MQ=60)
                             GGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAG                                                                 >  SRR4302535.851632/1‑94 (MQ=60)
                              GTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGC                                                                <  SRR4302535.4587561/94‑1 (MQ=60)
                               TTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCC                                                               >  SRR4302535.2336620/1‑94 (MQ=60)
                                 AGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCG                                                             >  SRR4302535.1878336/1‑94 (MQ=60)
                                 AGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCG                                                             >  SRR4302535.1133358/1‑94 (MQ=60)
                                 AGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCG                                                             >  SRR4302535.4212913/1‑94 (MQ=60)
                                  GATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGC                                                            >  SRR4302535.4643505/1‑94 (MQ=60)
                                   ATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCA                                                           >  SRR4302535.4576794/1‑94 (MQ=60)
                                      GTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCC                                                        >  SRR4302535.2786453/1‑94 (MQ=60)
                                       TGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCA                                                       >  SRR4302535.3045062/1‑94 (MQ=60)
                                        GGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAG                                                      <  SRR4302535.1346933/94‑1 (MQ=60)
                                          CGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGC                                                    >  SRR4302535.809291/1‑94 (MQ=60)
                                           GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCGGGCT                                                   >  SRR4302535.2599108/1‑94 (MQ=60)
                                           GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCT                                                   <  SRR4302535.1065295/94‑1 (MQ=60)
                                           GGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCT                                                   >  SRR4302535.2943465/1‑94 (MQ=60)
                                             TAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGG                                                 <  SRR4302535.3030180/94‑1 (MQ=60)
                                              AGCTGGCTGAAAGCCGGGCTGTATGGCTGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGG                                                <  SRR4302535.4700437/94‑1 (MQ=60)
                                              AGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGG                                                <  SRR4302535.248912/94‑1 (MQ=60)
                                              AGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGG                                                <  SRR4302535.1647436/94‑1 (MQ=60)
                                              AGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGG                                                >  SRR4302535.1923072/1‑94 (MQ=60)
                                              AGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGG                                                >  SRR4302535.309397/1‑94 (MQ=60)
                                               GCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGC                                               <  SRR4302535.4185148/94‑1 (MQ=60)
                                               GCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGC                                               <  SRR4302535.1685515/94‑1 (MQ=60)
                                                CTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCA                                              <  SRR4302535.4319442/94‑1 (MQ=60)
                                                 TGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGGAG                                             >  SRR4302535.545105/1‑94 (MQ=60)
                                                 TGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAG                                             >  SRR4302535.3157976/1‑94 (MQ=60)
                                                  GGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGA                                            >  SRR4302535.1423325/1‑94 (MQ=60)
                                                   GCTGAAAGCCGGGCTTAATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAG                                           <  SRR4302535.4423926/94‑1 (MQ=60)
                                                    CTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGC                                          <  SRR4302535.376939/94‑1 (MQ=60)
                                                     TGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCG                                         <  SRR4302535.1821289/94‑1 (MQ=60)
                                                     TGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCG                                         <  SRR4302535.2069307/94‑1 (MQ=60)
                                                     TGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCG                                         >  SRR4302535.2453582/1‑94 (MQ=60)
                                                      GAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGC                                        <  SRR4302535.3574502/94‑1 (MQ=60)
                                                        AAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGA                                      <  SRR4302535.3959270/94‑1 (MQ=60)
                                                           CCGGTCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAT                                   <  SRR4302535.645861/94‑1 (MQ=60)
                                                           CCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAT                                   <  SRR4302535.2755559/94‑1 (MQ=60)
                                                           CCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAT                                   <  SRR4302535.2755560/94‑1 (MQ=60)
                                                           CCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAT                                   <  SRR4302535.3197784/94‑1 (MQ=60)
                                                            CGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATG                                  >  SRR4302535.4051136/1‑94 (MQ=60)
                                                            CGGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCCCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATG                                  <  SRR4302535.2339304/94‑1 (MQ=60)
                                                             GGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGG                                 <  SRR4302535.2644163/94‑1 (MQ=60)
                                                             GGGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGG                                 >  SRR4302535.820024/1‑94 (MQ=60)
                                                              GGCTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGC                                <  SRR4302535.889831/94‑1 (MQ=60)
                                                               GCTGTATGCCCGCGAAGGCGGGGAGGCAGACGTGCAGCGCCTGCCGCTGGGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCA                               <  SRR4302535.1713260/94‑1 (MQ=60)
                                                                CTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAG                              >  SRR4302535.471605/1‑94 (MQ=60)
                                                                CTGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAG                              >  SRR4302535.422657/1‑94 (MQ=60)
                                                                 TGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGGTATGGCAGA                             >  SRR4302535.1513290/1‑94 (MQ=60)
                                                                 TGTATGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGA                             >  SRR4302535.4154783/1‑94 (MQ=60)
                                                                     TGACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTG                         <  SRR4302535.3784281/94‑1 (MQ=60)
                                                                      GACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGT                        >  SRR4302535.2701378/1‑94 (MQ=60)
                                                                      GACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAAATGT                        >  SRR4302535.3993873/1‑94 (MQ=60)
                                                                       ACCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTG                       <  SRR4302535.1150527/94‑1 (MQ=60)
                                                                        CCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGG                      <  SRR4302535.2216403/94‑1 (MQ=60)
                                                                        CCGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGG                      <  SRR4302535.4185692/94‑1 (MQ=60)
                                                                         CGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGC                     <  SRR4302535.2953190/94‑1 (MQ=60)
                                                                         CGCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGC                     <  SRR4302535.2725688/94‑1 (MQ=60)
                                                                          GCGAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCA                    <  SRR4302535.2893037/94‑1 (MQ=60)
                                                                            GAAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAT                  <  SRR4302535.2529209/94‑1 (MQ=60)
                                                                             AAGGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGAAGAACTGTGGCAATG                 >  SRR4302535.3136511/1‑94 (MQ=60)
                                                                               GGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCT               <  SRR4302535.4135497/94‑1 (MQ=60)
                                                                               GGCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCT               <  SRR4302535.539850/94‑1 (MQ=60)
                                                                                GCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTG              <  SRR4302535.3690158/94‑1 (MQ=60)
                                                                                GCCGGGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTG              <  SRR4302535.1984825/94‑1 (MQ=60)
                                                                                  CGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCA            >  SRR4302535.3001788/1‑94 (MQ=60)
                                                                                    GGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATG          <  SRR4302535.1849861/94‑1 (MQ=60)
                                                                                    GGAGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATG          <  SRR4302535.2193400/94‑1 (MQ=60)
                                                                                      AGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGC        <  SRR4302535.4376347/94‑1 (MQ=60)
                                                                                      AGGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGC        >  SRR4302535.2630506/1‑94 (MQ=60)
                                                                                       GGCAGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCT       <  SRR4302535.1594416/94‑1 (MQ=60)
                                                                                       GGCAGACGTGCAGCCCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCT       <  SRR4302535.675639/94‑1 (MQ=60)
                                                                                          AGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTC    <  SRR4302535.4450657/94‑1 (MQ=60)
                                                                                          AGACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTC    >  SRR4302535.3986823/1‑94 (MQ=60)
                                                                                           GACGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCA   <  SRR4302535.3126654/94‑1 (MQ=60)
                                                                                            GCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  <  SRR4302535.3053635/94‑1 (MQ=60)
                                                                                            |                                                                                             
GCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGTGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAT  >  NC_000913/3744704‑3744889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑