Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,744,958 G→A G82E (GGA→GAA)  lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,744,9580GA99.2% 901.6 / ‑5.4 251G82E (GGA→GAA) lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (110/139);  total (111/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 267 total aligned reads displayed.

GGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTC  >  NC_000913/3744868‑3745047
                                                                                          |                                                                                         
ggCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGaaa                                                                                         <  2:762671/93‑1 (MQ=255)
 gCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCTGCATCTCCGCACAGGaaaa                                                                                        <  2:1768442/93‑1 (MQ=255)
 gCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGaaaa                                                                                        <  2:2903338/93‑1 (MQ=255)
 gCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGaaaa                                                                                        <  1:2237011/93‑1 (MQ=255)
  cAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAg                                                                                       >  2:4219348/1‑93 (MQ=255)
  cAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAg                                                                                       <  2:4242770/93‑1 (MQ=255)
   aaTGCTGCATGGCTGTCATTCGCTCCTTGCTTACGCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAgg                                                                                      <  2:818464/93‑1 (MQ=255)
    aTGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAggg                                                                                     >  1:1200505/1‑93 (MQ=255)
    aTGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAggg                                                                                     <  2:799791/93‑1 (MQ=255)
    aTGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAggg                                                                                     >  2:2759297/1‑93 (MQ=255)
    aTGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAggg                                                                                     <  1:3037186/93‑1 (MQ=255)
    aTGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAggg                                                                                     <  1:1723955/93‑1 (MQ=255)
     tgctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGc                                                                                    >  2:717537/1‑93 (MQ=255)
      gctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCt                                                                                   <  2:3208739/93‑1 (MQ=255)
       ctgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCtt                                                                                  <  2:3425980/93‑1 (MQ=255)
        tgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTg                                                                                 >  1:18623/1‑93 (MQ=255)
        tgcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTg                                                                                 <  2:26582/93‑1 (MQ=255)
         gcATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGt                                                                                >  2:4126874/1‑93 (MQ=255)
          cATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtt                                                                               <  2:4179429/93‑1 (MQ=255)
          cATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtt                                                                               <  1:1570071/93‑1 (MQ=255)
           aTGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttt                                                                              >  2:4271162/1‑93 (MQ=255)
           aTGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttt                                                                              <  2:64022/93‑1 (MQ=255)
           aTGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttt                                                                              >  2:551663/1‑93 (MQ=255)
            tGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGtttt                                                                             <  2:4558709/93‑1 (MQ=255)
             ggCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttttt                                                                            <  1:4269643/93‑1 (MQ=255)
             ggCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttttt                                                                            <  2:2313567/93‑1 (MQ=255)
             ggCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGttttt                                                                            <  1:3616199/93‑1 (MQ=255)
              gCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGGACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTg                                                                           >  2:1147077/1‑93 (MQ=255)
                 gTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTg                                                                        >  2:3059672/1‑93 (MQ=255)
                 gTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTg                                                                        >  1:1925016/1‑93 (MQ=255)
                   cATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGa                                                                      <  1:2344608/93‑1 (MQ=255)
                       cgcgCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATaaa                                                                  <  1:1348989/93‑1 (MQ=255)
                        gcgcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATaaaa                                                                 <  2:3275091/93‑1 (MQ=255)
                        gcgcCCTGCTTACTCAGTCTGGTGTTAGCGGGGGACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTGTTTGCTGGCTaaaa                                                                 >  2:3613811/1‑93 (MQ=255)
                          gcgcTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGATGGATAAAAAc                                                               <  1:1493684/90‑1 (MQ=255)
                          gcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAc                                                               <  1:3292643/93‑1 (MQ=255)
                          gcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAc                                                               >  1:4420346/1‑93 (MQ=255)
                          gcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAc                                                               >  2:895320/1‑93 (MQ=255)
                          gcCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAc                                                               <  1:658734/93‑1 (MQ=255)
                          ccccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAc                                                               <  1:2457992/92‑1 (MQ=255)
                          ccccTGCTTACTCATTCTGGTGTTAGCGGCGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAc                                                               <  2:2989040/92‑1 (MQ=255)
                            ccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGa                                                             >  1:404881/1‑93 (MQ=255)
                            ccTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGa                                                             >  1:4138538/1‑93 (MQ=255)
                             cTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAc                                                            >  1:3148992/1‑93 (MQ=255)
                              tGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAATAACGACa                                                           >  1:927908/1‑93 (MQ=255)
                                cTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACaaa                                                         <  1:3333912/93‑1 (MQ=255)
                                 ttACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAc                                                        <  1:1009020/93‑1 (MQ=255)
                                    cTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGc                                                     <  1:1547588/93‑1 (MQ=255)
                                    cTCATTCTGGTGTTAGCGGAGAACAAATTGTCGGTATCGGCATCTCCGCAAAGGAAACGGTCTTGTTTTTGCTGGATAAAACCGCCAAACCGc                                                     <  2:1574650/93‑1 (MQ=255)
                                      cATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTc                                                   <  2:427417/93‑1 (MQ=255)
                                      cATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTc                                                   >  1:2161366/1‑93 (MQ=255)
                                       aTTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCg                                                  <  2:1345383/93‑1 (MQ=255)
                                        ttCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAACACGACAAACCGCTCgg                                                 >  2:4442504/1‑93 (MQ=255)
                                        ttCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCgt                                                 <  2:814314/93‑2 (MQ=255)
                                        ttCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCgg                                                 >  1:224841/1‑93 (MQ=255)
                                        ttCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCgg                                                 <  1:4625096/93‑1 (MQ=255)
                                        ttCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCgg                                                 >  2:362827/1‑93 (MQ=255)
                                          cTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGa                                               <  2:679227/93‑1 (MQ=255)
                                          cTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGa                                               >  1:473611/1‑93 (MQ=255)
                                           tGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGaa                                              <  2:1013106/93‑1 (MQ=255)
                                           tGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGaa                                              <  1:2153124/93‑1 (MQ=255)
                                            tgtgtTAGCGGGTAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAATCGACAAACCGGTCGGGAAt                                             <  2:4545710/92‑1 (MQ=255)
                                            ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAt                                             <  2:3926289/93‑1 (MQ=255)
                                            ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAt                                             <  1:4571314/93‑1 (MQ=255)
                                            ggTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAt                                             <  2:3136149/93‑1 (MQ=255)
                                             gtgtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATg                                            <  1:371011/93‑1 (MQ=255)
                                               gtTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCt                                          <  1:290784/93‑1 (MQ=255)
                                                  aGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAtt                                       <  1:1442770/93‑1 (MQ=255)
                                                  aGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAtt                                       <  1:4322643/93‑1 (MQ=255)
                                                  aGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAtt                                       <  2:192305/93‑1 (MQ=255)
                                                   gCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAttt                                      >  1:2076545/1‑93 (MQ=255)
                                                   gCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAttt                                      <  2:4627456/93‑1 (MQ=255)
                                                     ggggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTg                                    >  2:2252998/1‑93 (MQ=255)
                                                      gggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGt                                   >  1:2128318/1‑93 (MQ=255)
                                                      gggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGt                                   <  2:280439/93‑1 (MQ=255)
                                                      gggAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCACTCGGGAATGCTATTTTGt                                   >  2:379060/1‑93 (MQ=255)
                                                         aaCAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCt                                <  2:2691228/93‑1 (MQ=255)
                                                          aCAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTc                               <  1:177777/93‑1 (MQ=255)
                                                             aaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGa                            <  1:4172879/93‑1 (MQ=255)
                                                             aaTTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGa                            >  1:2639888/1‑93 (MQ=255)
                                                                 gTCGGTATCAGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAccgc                        <  2:1672203/93‑1 (MQ=255)
                                                                    ggTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCgg                     <  1:2782560/93‑1 (MQ=255)
                                                                     gTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCggg                    >  1:80535/1‑93 (MQ=255)
                                                                       aTCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCg                  >  2:4201714/1‑93 (MQ=255)
                                                                       aTCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCg                  <  1:3946294/93‑1 (MQ=255)
                                                                        tCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGa                 >  1:2597950/1‑93 (MQ=255)
                                                                          ggCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATg               <  2:1502481/93‑1 (MQ=255)
                                                                             aTCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaa            >  2:4005558/1‑93 (MQ=255)
                                                                             aTCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaa            <  2:2019321/93‑1 (MQ=255)
                                                                             aTCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaa            >  2:1894337/1‑93 (MQ=255)
                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaaa           >  2:3321906/1‑93 (MQ=255)
                                                                              tctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGaaa           <  1:251780/93‑1 (MQ=255)
                                                                               ctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAt          <  2:1413239/93‑1 (MQ=255)
                                                                               ctcCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAt          <  1:3503483/93‑1 (MQ=255)
                                                                               ctcCGCACAGGAAAAGGGCTTGTTTTTGCGGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAt          <  2:3799309/93‑1 (MQ=255)
                                                                                 ccGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcg        >  1:3766587/1‑93 (MQ=255)
                                                                                   gCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgtt      <  2:2189484/93‑1 (MQ=255)
                                                                                   gCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgtt      <  1:984976/93‑1 (MQ=255)
                                                                                     acaGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAtcgttcg    >  2:951978/1‑93 (MQ=255)
                                                                                       aGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTtcgtc  <  2:3699878/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTC  >  NC_000913/3744868‑3745047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTG  >  NC_000913/3744866‑3745051
                                                                                            |                                                                                             
GTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAA                                                                                              >  SRR4302535.1002720/1‑94 (MQ=60)
GTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAA                                                                                              <  SRR4302535.2960536/94‑1 (MQ=60)
GTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAA                                                                                              <  SRR4302535.4119616/94‑1 (MQ=60)
   GCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAG                                                                                           <  SRR4302535.2252923/94‑1 (MQ=60)
     AATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGG                                                                                         >  SRR4302535.1209106/1‑94 (MQ=60)
     AATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGG                                                                                         >  SRR4302535.90872/1‑94 (MQ=60)
     AATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGG                                                                                         <  SRR4302535.573417/94‑1 (MQ=60)
      ATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGC                                                                                        <  SRR4302535.3058457/94‑1 (MQ=60)
      ATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGC                                                                                        <  SRR4302535.1736423/94‑1 (MQ=60)
       TGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCT                                                                                       <  SRR4302535.1805844/94‑1 (MQ=60)
       TGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCT                                                                                       <  SRR4302535.4170432/94‑1 (MQ=60)
        GCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTT                                                                                      <  SRR4302535.2911178/94‑1 (MQ=60)
        GCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTT                                                                                      <  SRR4302535.3545167/94‑1 (MQ=60)
         CTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTG                                                                                     >  SRR4302535.18761/1‑94 (MQ=60)
           GCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTT                                                                                   <  SRR4302535.4517724/94‑1 (MQ=60)
            CATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTT                                                                                  <  SRR4302535.1581405/94‑1 (MQ=60)
            CATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTT                                                                                  <  SRR4302535.1394056/94‑1 (MQ=60)
              TGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTT                                                                                <  SRR4302535.1432650/94‑1 (MQ=60)
               GGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTG                                                                               <  SRR4302535.4298875/94‑1 (MQ=60)
               GGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTG                                                                               <  SRR4302535.2106304/94‑1 (MQ=60)
               GGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTG                                                                               <  SRR4302535.2106303/94‑1 (MQ=60)
               GGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTG                                                                               <  SRR4302535.3641266/94‑1 (MQ=60)
                  TGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTG                                                                            >  SRR4302535.1938894/1‑94 (MQ=60)
                   GTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGG                                                                           >  SRR4302535.3812790/1‑94 (MQ=60)
                    GCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATTGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGA                                                                          <  SRR4302535.673805/94‑1 (MQ=60)
                     CATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGAT                                                                         <  SRR4302535.2361406/94‑1 (MQ=60)
                     CATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGAT                                                                         >  SRR4302535.54983/1‑94 (MQ=60)
                     CATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGAT                                                                         <  SRR4302535.1777717/94‑1 (MQ=60)
                     CATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGCGCTTGTTTTTGCTGGAT                                                                         <  SRR4302535.2434719/94‑1 (MQ=60)
                         CGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAA                                                                     <  SRR4302535.1358623/94‑1 (MQ=60)
                         CGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAA                                                                     >  SRR4302535.3184728/1‑94 (MQ=60)
                          GCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAA                                                                    >  SRR4302535.191365/1‑94 (MQ=60)
                           CGCGCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAC                                                                   <  SRR4302535.1997861/94‑1 (MQ=60)
                           CGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAAC                                                                   >  SRR4302535.4450611/1‑94 (MQ=60)
                            GCGCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGATGGATAAAAACG                                                                  <  SRR4302535.1504416/94‑1 (MQ=60)
                            GCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACG                                                                  <  SRR4302535.2174302/94‑1 (MQ=60)
                            GCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACG                                                                  <  SRR4302535.3315536/94‑1 (MQ=60)
                            GCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACG                                                                  <  SRR4302535.663332/94‑1 (MQ=60)
                            CCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACG                                                                  <  SRR4302535.2475719/94‑1 (MQ=60)
                             CCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGA                                                                 >  SRR4302535.407716/1‑94 (MQ=60)
                             CCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGA                                                                 >  SRR4302535.94459/1‑94 (MQ=60)
                             CCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGA                                                                 >  SRR4302535.4166881/1‑94 (MQ=60)
                              CCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGAC                                                                >  SRR4302535.3170884/1‑94 (MQ=60)
                               CTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAATAACGACA                                                               >  SRR4302535.934479/1‑94 (MQ=60)
                                 GCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAA                                                             >  SRR4302535.2844154/1‑94 (MQ=60)
                                  CTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAC                                                            <  SRR4302535.3357134/94‑1 (MQ=60)
                                  CTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAC                                                            >  SRR4302535.4340076/1‑94 (MQ=60)
                                  CTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAC                                                            >  SRR4302535.1570629/1‑94 (MQ=60)
                                  CTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAAC                                                            >  SRR4302535.2375033/1‑94 (MQ=60)
                                   TTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACC                                                           <  SRR4302535.3156288/94‑1 (MQ=60)
                                   TTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACC                                                           <  SRR4302535.1016190/94‑1 (MQ=60)
                                    TACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCG                                                          <  SRR4302535.4605543/94‑1 (MQ=60)
                                    TACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCG                                                          >  SRR4302535.990963/1‑94 (MQ=60)
                                     ACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGC                                                         >  SRR4302535.2619138/1‑94 (MQ=60)
                                      CTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCT                                                        <  SRR4302535.1558734/94‑1 (MQ=60)
                                      CTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCT                                                        >  SRR4302535.1127983/1‑94 (MQ=60)
                                       TCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTC                                                       >  SRR4302535.2176880/1‑94 (MQ=60)
                                       TCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTC                                                       <  SRR4302535.2610437/94‑1 (MQ=60)
                                         ATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGG                                                     >  SRR4302535.665251/1‑94 (MQ=60)
                                         ATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGG                                                     >  SRR4302535.226390/1‑94 (MQ=60)
                                         ATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGG                                                     >  SRR4302535.3695811/1‑94 (MQ=60)
                                          TTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGG                                                    <  SRR4302535.4656499/94‑1 (MQ=60)
                                          TTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGG                                                    <  SRR4302535.1769726/94‑1 (MQ=60)
                                           TCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGA                                                   >  SRR4302535.2348885/1‑94 (MQ=60)
                                           TCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGA                                                   >  SRR4302535.476926/1‑94 (MQ=60)
                                            CTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAA                                                  <  SRR4302535.2600104/94‑1 (MQ=60)
                                             TGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAT                                                 <  SRR4302535.4200773/94‑1 (MQ=60)
                                             TGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAAT                                                 <  SRR4302535.2168599/94‑1 (MQ=60)
                                              GGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATG                                                <  SRR4302535.4602481/94‑1 (MQ=60)
                                              GGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATG                                                <  SRR4302535.3019487/94‑1 (MQ=60)
                                               GTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGC                                               >  SRR4302535.1731889/1‑94 (MQ=60)
                                               GTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGC                                               <  SRR4302535.373578/94‑1 (MQ=60)
                                                 GTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTA                                             <  SRR4302535.292819/94‑1 (MQ=60)
                                                  TTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTAT                                            <  SRR4302535.93109/94‑1 (MQ=60)
                                                    AGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTT                                          <  SRR4302535.4352215/94‑1 (MQ=60)
                                                    AGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTT                                          >  SRR4302535.2091505/1‑94 (MQ=60)
                                                    AGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTT                                          <  SRR4302535.1453091/94‑1 (MQ=60)
                                                       GGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGT                                       >  SRR4302535.2143664/1‑94 (MQ=60)
                                                         GGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGCCAAACCGCTCGGGAATGCTATTTTGTCC                                     >  SRR4302535.1474268/1‑94 (MQ=60)
                                                         GGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCC                                     <  SRR4302535.3791085/94‑1 (MQ=60)
                                                         GGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCC                                     <  SRR4302535.2175495/94‑1 (MQ=60)
                                                         GGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCC                                     >  SRR4302535.2028237/1‑94 (MQ=60)
                                                         GGAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCC                                     >  SRR4302535.2512878/1‑94 (MQ=60)
                                                          GAACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCT                                    >  SRR4302535.4703542/1‑94 (MQ=60)
                                                           AACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGCATGCTATTTTGTCCTC                                   >  SRR4302535.4407335/1‑94 (MQ=60)
                                                            ACAGATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCG                                  <  SRR4302535.21449/94‑1 (MQ=60)
                                                            ACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCG                                  <  SRR4302535.179012/94‑1 (MQ=60)
                                                            ACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCG                                  <  SRR4302535.4423201/94‑1 (MQ=60)
                                                            ACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCG                                  <  SRR4302535.3407360/94‑1 (MQ=60)
                                                            ACAAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCG                                  >  SRR4302535.1326872/1‑94 (MQ=60)
                                                              AAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGA                                >  SRR4302535.4671385/1‑94 (MQ=60)
                                                              AAATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGA                                >  SRR4302535.2658800/1‑94 (MQ=60)
                                                               AATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAC                               <  SRR4302535.4201439/94‑1 (MQ=60)
                                                               AATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGAC                               <  SRR4302535.2322152/94‑1 (MQ=60)
                                                                ATTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACC                              >  SRR4302535.2985845/1‑94 (MQ=60)
                                                                 TTGTCGGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCG                             <  SRR4302535.2228015/94‑1 (MQ=60)
                                                                     CGGTATCGGCATCTCCGCACAGAAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGG                         <  SRR4302535.3332749/94‑1 (MQ=60)
                                                                      GGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGG                        >  SRR4302535.81108/1‑94 (MQ=60)
                                                                      GGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGG                        >  SRR4302535.992393/1‑94 (MQ=60)
                                                                      GGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGG                        <  SRR4302535.2802365/94‑1 (MQ=60)
                                                                      GGTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGG                        >  SRR4302535.3638161/1‑94 (MQ=60)
                                                                       GTATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGC                       >  SRR4302535.217823/1‑94 (MQ=60)
                                                                         ATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGA                     >  SRR4302535.1812842/1‑94 (MQ=60)
                                                                         ATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGA                     >  SRR4302535.2616610/1‑94 (MQ=60)
                                                                         ATCGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGA                     <  SRR4302535.3973242/94‑1 (MQ=60)
                                                                           CGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATG                   <  SRR4302535.3300611/94‑1 (MQ=60)
                                                                           CGGCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATG                   <  SRR4302535.1560006/94‑1 (MQ=60)
                                                                             GCATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGA                 <  SRR4302535.1507194/94‑1 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                <  SRR4302535.3330782/94‑1 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                >  SRR4302535.4129862/1‑94 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                >  SRR4302535.3983068/1‑94 (MQ=60)
                                                                              CATCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAA                >  SRR4302535.4003330/1‑94 (MQ=60)
                                                                                TCTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAAT              <  SRR4302535.253513/94‑1 (MQ=60)
                                                                                 CTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATC             <  SRR4302535.3527834/94‑1 (MQ=60)
                                                                                 CTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATC             >  SRR4302535.3813380/1‑94 (MQ=60)
                                                                                 CTCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATC             <  SRR4302535.4292047/94‑1 (MQ=60)
                                                                                  TCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGCCCGCCGGGCGATGGAAATCG            <  SRR4302535.266074/94‑1 (MQ=60)
                                                                                  TCCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCG            >  SRR4302535.3792599/1‑94 (MQ=60)
                                                                                   CCGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGT           <  SRR4302535.4589514/94‑1 (MQ=60)
                                                                                    CGCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTT          <  SRR4302535.1289883/94‑1 (MQ=60)
                                                                                     GCACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTC         <  SRR4302535.991974/94‑1 (MQ=60)
                                                                                      CACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCG        >  SRR4302535.263735/1‑94 (MQ=60)
                                                                                      CACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCG        >  SRR4302535.3655355/1‑94 (MQ=60)
                                                                                       ACAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGT       <  SRR4302535.1511707/94‑1 (MQ=60)
                                                                                        CAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTC      <  SRR4302535.4257699/94‑1 (MQ=60)
                                                                                        CAGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTC      >  SRR4302535.2264278/1‑94 (MQ=60)
                                                                                         AGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCG     >  SRR4302535.1337141/1‑94 (MQ=60)
                                                                                         AGGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCG     >  SRR4302535.888094/1‑94 (MQ=60)
                                                                                          GGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGC    >  SRR4302535.3179072/1‑94 (MQ=60)
                                                                                          GGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGC    <  SRR4302535.3632810/94‑1 (MQ=60)
                                                                                          GGAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTAGTCGC    >  SRR4302535.989161/1‑94 (MQ=60)
                                                                                           GAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCT   <  SRR4302535.120533/94‑1 (MQ=60)
                                                                                           GAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCT   <  SRR4302535.3679325/94‑1 (MQ=60)
                                                                                           GAAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCT   >  SRR4302535.2285658/1‑94 (MQ=60)
                                                                                            AAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTG  >  SRR4302535.2244210/1‑94 (MQ=60)
                                                                                            |                                                                                             
GTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCATCTCCGCACAGGGAAAGGGCTTGTTTTTGCTGGATAAAAACGACAAACCGCTCGGGAATGCTATTTTGTCCTCGGACCGCCGGGCGATGGAAATCGTTCGTCGCTG  >  NC_000913/3744866‑3745051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑