Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,820,538 A→G H171R (CAC→CGC)  spoT → (p)ppGpp synthetase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,820,5380AG99.6% 699.2 / ‑5.7 226H171R (CAC→CGC) spoT(p)ppGpp synthetase II
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (110/114);  total (111/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 240 total aligned reads displayed.

CCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAG  >  NC_000913/3820448‑3820627
                                                                                          |                                                                                         
cccACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGcc                                                                                         >  2:2459255/1‑93 (MQ=255)
 ccACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAACCGTCGCCGCCTCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCg                                                                                        >  1:1007436/1‑93 (MQ=255)
 ccACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCg                                                                                        >  2:2607081/1‑93 (MQ=255)
 ccACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCg                                                                                        >  1:3344007/1‑93 (MQ=255)
   acaacaTGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGtt                                                                                      <  2:1207477/93‑1 (MQ=255)
   acaacaTGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGtt                                                                                      >  1:2326235/1‑93 (MQ=255)
   acaacaTGCGCACGCCGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCTGTGAAACTCTCGATATTTATAGCCCGCTGGCGCGCCGtt                                                                                      >  2:3638516/1‑93 (MQ=255)
    caacaTGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGttt                                                                                     >  2:1431380/1‑93 (MQ=255)
    caacaTGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGttt                                                                                     >  2:3767797/1‑93 (MQ=255)
    caacaTGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGttt                                                                                     >  2:629901/1‑93 (MQ=255)
      acaTGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAg                                                                                   >  1:3112015/1‑93 (MQ=255)
      acaTGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAg                                                                                   >  2:1401343/1‑93 (MQ=255)
       caTGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAgg                                                                                  <  1:1708864/93‑1 (MQ=255)
         tGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTa                                                                                <  2:4153557/93‑1 (MQ=255)
         tGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCATTTAGGTa                                                                                <  2:4569850/93‑1 (MQ=255)
          gcgcACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTAt                                                                               <  1:4546577/93‑1 (MQ=255)
          gcgcACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTAt                                                                               <  2:3997965/93‑1 (MQ=255)
          gcgcACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTAt                                                                               <  1:2748874/93‑1 (MQ=255)
            gcACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGGCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATcc                                                                             <  1:4414669/93‑1 (MQ=255)
            gcACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATcc                                                                             >  1:3974089/1‑93 (MQ=255)
            gcACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATcc                                                                             >  2:4638285/1‑93 (MQ=255)
               cGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATccacc                                                                          >  2:4249508/1‑93 (MQ=255)
                ccTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATccacca                                                                         <  1:4504191/92‑1 (MQ=255)
                  tGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACcaca                                                                       >  1:1586463/1‑93 (MQ=255)
                  tGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGGGCAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACcaca                                                                       >  2:3320267/1‑93 (MQ=255)
                   gggCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACAt                                                                      <  2:2118459/93‑1 (MQ=255)
                    ggCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACAtt                                                                     >  1:2458016/1‑93 (MQ=255)
                    ggCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACAtt                                                                     <  1:873142/93‑1 (MQ=255)
                      cTCACTTCGCCCGGACAGACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTaa                                                                   >  1:4173176/1‑93 (MQ=255)
                      cTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTaa                                                                   <  1:1228905/93‑1 (MQ=255)
                      cTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTaa                                                                   <  1:3440854/93‑1 (MQ=255)
                      cTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTaa                                                                   <  1:3005487/93‑1 (MQ=255)
                      cTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTaa                                                                   <  2:1053992/93‑1 (MQ=255)
                      cTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTaa                                                                   >  2:3213694/1‑93 (MQ=255)
                         aCTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAAc                                                                <  1:574744/93‑1 (MQ=255)
                         aCTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAAc                                                                <  2:4332908/93‑1 (MQ=255)
                          cTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAAcc                                                               <  1:147922/93‑1 (MQ=255)
                          cTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAAcc                                                               >  1:2074633/1‑93 (MQ=255)
                          cTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAAcc                                                               <  2:2267344/93‑1 (MQ=255)
                          cTTCGCCCAGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAAcc                                                               <  2:93190/93‑1 (MQ=255)
                           ttCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCCCCACATTAAAACCg                                                              >  2:3023550/1‑93 (MQ=255)
                           ttCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTGAAACCg                                                              >  2:4359578/1‑93 (MQ=255)
                           ttCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCg                                                              >  1:3791816/1‑93 (MQ=255)
                           ttCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCg                                                              >  2:1296323/1‑93 (MQ=255)
                            tCGCCCGGACAAACGTCGCCTCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGa                                                             <  2:1918655/93‑1 (MQ=255)
                              gCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAAc                                                           <  2:3173259/93‑1 (MQ=255)
                              gCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAAc                                                           <  2:2751555/93‑1 (MQ=255)
                                ccGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTc                                                         <  2:873339/93‑1 (MQ=255)
                                  ggACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGCACTCGa                                                       >  1:3233453/1‑93 (MQ=255)
                                  ggACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGa                                                       <  1:3660577/93‑1 (MQ=255)
                                  ggACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGAGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGa                                                       <  2:3141035/93‑1 (MQ=255)
                                   gACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGaa                                                      <  2:1668844/93‑1 (MQ=255)
                                   gACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGaa                                                      >  1:2189164/1‑93 (MQ=255)
                                   gACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGaa                                                      >  1:3630698/1‑93 (MQ=255)
                                    aCAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAag                                                     <  1:2890280/93‑1 (MQ=255)
                                     cAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAaga                                                    <  1:1868102/93‑1 (MQ=255)
                                     cAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAaga                                                    <  2:2715746/93‑1 (MQ=255)
                                      aaccGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTGAGGTATCCCCCACATTAAAACCGAACGCGAagag                                                   <  2:2850186/90‑1 (MQ=255)
                                       aaCGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGc                                                  >  1:664526/1‑93 (MQ=255)
                                          gTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTgg                                               <  2:2217270/93‑1 (MQ=255)
                                          gTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTgg                                               <  1:3573683/93‑1 (MQ=255)
                                           tCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTggg                                              <  2:738806/93‑1 (MQ=255)
                                           tCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTggg                                              <  2:3516363/93‑1 (MQ=255)
                                           tCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTggg                                              >  1:114136/1‑93 (MQ=255)
                                           tCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTggg                                              >  1:2356025/1‑93 (MQ=255)
                                               cgcATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGtttt                                          >  1:1624898/1‑93 (MQ=255)
                                                 cATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGa                                        <  2:2080138/93‑1 (MQ=255)
                                                  aTCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGat                                       >  1:4430609/1‑92 (MQ=255)
                                                  aTCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAg                                       <  1:2706102/93‑1 (MQ=255)
                                                  aTCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAg                                       <  1:2306899/93‑1 (MQ=255)
                                                    cGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGgc                                     >  2:3944412/1‑93 (MQ=255)
                                                      cccGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGgcgc                                   <  1:527490/93‑1 (MQ=255)
                                                       ccGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCt                                  >  2:3819878/1‑93 (MQ=255)
                                                       ccGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCt                                  >  1:2654694/1‑93 (MQ=255)
                                                       ccGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCt                                  <  1:4469832/93‑1 (MQ=255)
                                                       ccGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCt                                  >  1:1495633/1‑93 (MQ=255)
                                                        cGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTg                                 >  2:3581843/1‑93 (MQ=255)
                                                          tGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTa                               >  1:4649728/1‑93 (MQ=255)
                                                          tGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTa                               <  1:799376/93‑1 (MQ=255)
                                                          tGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTa                               >  2:1790744/1‑93 (MQ=255)
                                                           gAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTAt                              >  1:1611822/1‑93 (MQ=255)
                                                            aaaCTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTCAGGCGCTGTATc                             <  1:2533528/93‑1 (MQ=255)
                                                             aaCTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATcc                            >  1:4326235/1‑93 (MQ=255)
                                                             aaCTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATcc                            >  2:2418271/1‑93 (MQ=255)
                                                               ctctCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCa                          >  2:223745/1‑93 (MQ=255)
                                                                tctcGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCaa                         <  2:1856261/93‑1 (MQ=255)
                                                                 ctcGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAAc                        <  1:3924359/93‑1 (MQ=255)
                                                                  tcGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAAcc                       <  1:3709597/93‑1 (MQ=255)
                                                                     aaaTTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGtt                    >  1:4052369/1‑93 (MQ=255)
                                                                        tttATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATc                 >  1:2866368/1‑93 (MQ=255)
                                                                        tttATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATc                 >  2:4487486/1‑93 (MQ=255)
                                                                           ataGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATcgcg              <  1:1359891/93‑1 (MQ=255)
                                                                                ccGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATc         <  2:3416097/93‑1 (MQ=255)
                                                                                ccGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATc         >  1:1336630/1‑93 (MQ=255)
                                                                                  ccTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCaa       <  2:1720393/92‑1 (MQ=255)
                                                                                   gtGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCaaa      <  2:3656560/92‑1 (MQ=255)
                                                                                   cTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCaaa      >  1:1830774/1‑93 (MQ=255)
                                                                                   cTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCaaa      >  2:3091571/1‑93 (MQ=255)
                                                                                    tGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAaag     >  1:4260968/1‑93 (MQ=255)
                                                                                     ggCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAaaga    >  2:1591879/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAG  >  NC_000913/3820448‑3820627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGT  >  NC_000913/3820445‑3820631
                                                                                             |                                                                                             
GTACCCAGAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCG                                                                                               >  SRR4302535.1967395/1‑94 (MQ=60)
GTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCG                                                                                               <  SRR4302535.1753524/94‑1 (MQ=60)
  ACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCC                                                                                             <  SRR4302535.4194472/94‑1 (MQ=60)
   CCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAACCGTCGCCGCCTCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCG                                                                                            >  SRR4302535.1014594/1‑94 (MQ=60)
   CCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCG                                                                                            >  SRR4302535.3367303/1‑94 (MQ=60)
     CACACCATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGCCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTT                                                                                          <  SRR4302535.1284003/94‑1 (MQ=60)
     CACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTT                                                                                          <  SRR4302535.1783213/94‑1 (MQ=60)
     CACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTT                                                                                          >  SRR4302535.2342878/1‑94 (MQ=60)
      ACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTT                                                                                         >  SRR4302535.582159/1‑94 (MQ=60)
       CAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTA                                                                                        >  SRR4302535.4683069/1‑94 (MQ=60)
       CAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTA                                                                                        <  SRR4302535.1940169/94‑1 (MQ=60)
       CAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTA                                                                                        <  SRR4302535.4262616/94‑1 (MQ=60)
       CAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTA                                                                                        <  SRR4302535.2681582/94‑1 (MQ=60)
       CAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTA                                                                                        <  SRR4302535.4477559/94‑1 (MQ=60)
       CAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTA                                                                                        <  SRR4302535.3872210/94‑1 (MQ=60)
        AACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAG                                                                                       >  SRR4302535.3133679/1‑94 (MQ=60)
          CATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGT                                                                                     <  SRR4302535.1721218/94‑1 (MQ=60)
           ATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTA                                                                                    >  SRR4302535.4556147/1‑94 (MQ=60)
            TGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTAT                                                                                   <  SRR4302535.2335644/94‑1 (MQ=60)
            TGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTAT                                                                                   <  SRR4302535.1227656/94‑1 (MQ=60)
             GCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATC                                                                                  <  SRR4302535.2768471/94‑1 (MQ=60)
             GCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATC                                                                                  <  SRR4302535.4577664/94‑1 (MQ=60)
              CGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCC                                                                                 >  SRR4302535.4001234/1‑94 (MQ=60)
               GCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGGCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCA                                                                                <  SRR4302535.4444883/94‑1 (MQ=60)
               GCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCA                                                                                <  SRR4302535.2560738/94‑1 (MQ=60)
               GCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCA                                                                                <  SRR4302535.1640042/94‑1 (MQ=60)
                   CCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCAC                                                                            <  SRR4302535.4535117/94‑1 (MQ=60)
                    CTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACA                                                                           >  SRR4302535.1597911/1‑94 (MQ=60)
                      GGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATT                                                                         >  SRR4302535.2475743/1‑94 (MQ=60)
                       GGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTA                                                                        <  SRR4302535.879331/94‑1 (MQ=60)
                        GCTCACTTCGCCCGGACAGACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAA                                                                       >  SRR4302535.4201740/1‑94 (MQ=60)
                        GCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAA                                                                       <  SRR4302535.4421002/94‑1 (MQ=60)
                         CTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAA                                                                      <  SRR4302535.3464782/94‑1 (MQ=60)
                         CTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAA                                                                      <  SRR4302535.1237685/94‑1 (MQ=60)
                         CTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAA                                                                      <  SRR4302535.3026549/94‑1 (MQ=60)
                          TCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAA                                                                     <  SRR4302535.372603/94‑1 (MQ=60)
                           CACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAAC                                                                    >  SRR4302535.4487315/1‑94 (MQ=60)
                            ACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACC                                                                   <  SRR4302535.578779/94‑1 (MQ=60)
                            ACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACC                                                                   >  SRR4302535.2494092/1‑94 (MQ=60)
                            ACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACC                                                                   <  SRR4302535.2347453/94‑1 (MQ=60)
                            ACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACC                                                                   >  SRR4302535.2089579/1‑94 (MQ=60)
                            ACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACC                                                                   >  SRR4302535.4073966/1‑94 (MQ=60)
                             CTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCG                                                                  >  SRR4302535.4165441/1‑94 (MQ=60)
                             CTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCG                                                                  >  SRR4302535.3817958/1‑94 (MQ=60)
                             CTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCG                                                                  <  SRR4302535.148955/94‑1 (MQ=60)
                                  CCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTC                                                             >  SRR4302535.859708/1‑94 (MQ=60)
                                    CGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGCACTCGA                                                           >  SRR4302535.3255892/1‑94 (MQ=60)
                                    CGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGA                                                           >  SRR4302535.2943445/1‑94 (MQ=60)
                                     GGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAA                                                          <  SRR4302535.3685913/94‑1 (MQ=60)
                                     GGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAA                                                          >  SRR4302535.2204754/1‑94 (MQ=60)
                                     GGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAA                                                          >  SRR4302535.3655865/1‑94 (MQ=60)
                                      GACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAG                                                         >  SRR4302535.70578/1‑94 (MQ=60)
                                      GACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAG                                                         <  SRR4302535.2602219/94‑1 (MQ=60)
                                       ACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGA                                                        <  SRR4302535.2910728/94‑1 (MQ=60)
                                        CAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAG                                                       <  SRR4302535.1881565/94‑1 (MQ=60)
                                         AAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGC                                                      >  SRR4302535.4354837/1‑94 (MQ=60)
                                         AAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGC                                                      >  SRR4302535.669169/1‑94 (MQ=60)
                                           ACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTG                                                    <  SRR4302535.4310007/94‑1 (MQ=60)
                                             GTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGG                                                  <  SRR4302535.3598462/94‑1 (MQ=60)
                                             GTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGG                                                  >  SRR4302535.2372919/1‑94 (MQ=60)
                                             GTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGG                                                  >  SRR4302535.114937/1‑94 (MQ=60)
                                              TCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGT                                                 <  SRR4302535.367279/94‑1 (MQ=60)
                                              TCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGT                                                 <  SRR4302535.3767765/94‑1 (MQ=60)
                                                 CCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTT                                              >  SRR4302535.1636631/1‑94 (MQ=60)
                                                 CCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTT                                              <  SRR4302535.1621638/94‑1 (MQ=60)
                                                  CGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTG                                             <  SRR4302535.3974918/94‑1 (MQ=60)
                                                   GCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGA                                            >  SRR4302535.4526863/1‑94 (MQ=60)
                                                   GCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGA                                            <  SRR4302535.3482625/94‑1 (MQ=60)
                                                   GCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGA                                            >  SRR4302535.2188199/1‑94 (MQ=60)
                                                    CATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAT                                           >  SRR4302535.4460967/1‑94 (MQ=60)
                                                    CATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAG                                           >  SRR4302535.2762942/1‑94 (MQ=60)
                                                    CATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAG                                           >  SRR4302535.71991/1‑94 (MQ=60)
                                                     ATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGG                                          >  SRR4302535.2866959/1‑94 (MQ=60)
                                                     ATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGG                                          <  SRR4302535.2725440/94‑1 (MQ=60)
                                                     ATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGG                                          <  SRR4302535.328526/94‑1 (MQ=60)
                                                     ATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGG                                          <  SRR4302535.2323398/94‑1 (MQ=60)
                                                      TCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGC                                         <  SRR4302535.545787/94‑1 (MQ=60)
                                                      TCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGC                                         <  SRR4302535.3630902/94‑1 (MQ=60)
                                                        GCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGC                                       >  SRR4302535.4092065/1‑94 (MQ=60)
                                                         CCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCT                                      >  SRR4302535.351877/1‑94 (MQ=60)
                                                         CCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCT                                      <  SRR4302535.1494685/94‑1 (MQ=60)
                                                         CCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCT                                      >  SRR4302535.2673715/1‑94 (MQ=60)
                                                         CCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCT                                      >  SRR4302535.1506389/1‑94 (MQ=60)
                                                         CCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCT                                      <  SRR4302535.531175/94‑1 (MQ=60)
                                                          CCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTG                                     <  SRR4302535.4500501/94‑1 (MQ=60)
                                                           CGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGT                                    >  SRR4302535.2227379/1‑94 (MQ=60)
                                                            GTGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTA                                   >  SRR4302535.4681219/1‑94 (MQ=60)
                                                            GTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTA                                   <  SRR4302535.3008605/94‑1 (MQ=60)
                                                             TGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTAT                                  >  SRR4302535.1385270/1‑94 (MQ=60)
                                                             TGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTAT                                  >  SRR4302535.1623443/1‑94 (MQ=60)
                                                             TGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTAT                                  <  SRR4302535.804988/94‑1 (MQ=60)
                                                             TGAAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTAT                                  >  SRR4302535.322102/1‑94 (MQ=60)
                                                               AAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCC                                <  SRR4302535.1090028/94‑1 (MQ=60)
                                                               AAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCC                                >  SRR4302535.4355817/1‑94 (MQ=60)
                                                               AAACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTCAGGCGCTGTATCC                                <  SRR4302535.2551778/94‑1 (MQ=60)
                                                                AACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCC                               >  SRR4302535.2017681/1‑94 (MQ=60)
                                                                 ACTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCA                              <  SRR4302535.2800924/94‑1 (MQ=60)
                                                                  CTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCTACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAA                             >  SRR4302535.4560721/1‑94 (MQ=60)
                                                                  CTCTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAA                             >  SRR4302535.897207/1‑94 (MQ=60)
                                                                    CTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGCGCTGGGATTTGATGCGCTGTATCCCAAAC                           >  SRR4302535.2637373/1‑94 (MQ=60)
                                                                    CTCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACC                           <  SRR4302535.3951176/94‑1 (MQ=60)
                                                                     TCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCG                          <  SRR4302535.1636117/94‑1 (MQ=60)
                                                                     TCGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCG                          <  SRR4302535.3735288/94‑1 (MQ=60)
                                                                      CGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGT                         >  SRR4302535.2454975/1‑94 (MQ=60)
                                                                      CGAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGT                         >  SRR4302535.4623422/1‑94 (MQ=60)
                                                                       GAAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTT                        >  SRR4302535.4080114/1‑94 (MQ=60)
                                                                        AAATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTA                       >  SRR4302535.3272792/1‑94 (MQ=60)
                                                                          ATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATC                     >  SRR4302535.3059910/1‑94 (MQ=60)
                                                                          ATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATC                     >  SRR4302535.1409501/1‑94 (MQ=60)
                                                                          ATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATC                     >  SRR4302535.2886672/1‑94 (MQ=60)
                                                                          ATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATC                     <  SRR4302535.54006/94‑1 (MQ=60)
                                                                          ATTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATC                     >  SRR4302535.165564/1‑94 (MQ=60)
                                                                           TTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCG                    >  SRR4302535.1852031/1‑94 (MQ=60)
                                                                           TTTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCG                    <  SRR4302535.2385250/94‑1 (MQ=60)
                                                                            TTATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTaacccg                   >  SRR4302535.4067556/1‑88 (MQ=60)
                                                                             TATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCG                  >  SRR4302535.3943441/1‑94 (MQ=60)
                                                                             TATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTNANCGCG                  >  SRR4302535.4061494/1‑94 (MQ=60)
                                                                              ATAGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGT                 <  SRR4302535.1369616/94‑1 (MQ=60)
                                                                                AGCCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAA               <  SRR4302535.3445037/94‑1 (MQ=60)
                                                                                  CCCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATC             >  SRR4302535.1346165/1‑94 (MQ=60)
                                                                                   CCGCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCA            <  SRR4302535.1356897/94‑1 (MQ=60)
                                                                                     GCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCACA          >  SRR4302535.2232471/1‑94 (MQ=60)
                                                                                     GCTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAA          >  SRR4302535.1843988/1‑94 (MQ=60)
                                                                                      CTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCTCTGTATCCCAACCGTTATCGCGTAATCAAAG         <  SRR4302535.1604002/94‑1 (MQ=60)
                                                                                      CTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAG         <  SRR4302535.1528584/94‑1 (MQ=60)
                                                                                      CTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAG         >  SRR4302535.371467/1‑94 (MQ=60)
                                                                                      CTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAG         <  SRR4302535.1324127/94‑1 (MQ=60)
                                                                                      CTGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAG         >  SRR4302535.4290151/1‑94 (MQ=60)
                                                                                       TGGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGA        >  SRR4302535.2888489/1‑94 (MQ=60)
                                                                                        GGCGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAA       <  SRR4302535.4444902/94‑1 (MQ=60)
                                                                                          CGCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGT     <  SRR4302535.1274410/94‑1 (MQ=60)
                                                                                           GCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTG    >  SRR4302535.4069809/1‑94 (MQ=60)
                                                                                           GCGCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCAGTATCCCAACCGTTATCGCGTAATCAAAGAAGTG    <  SRR4302535.3691800/94‑1 (MQ=60)
                                                                                             GCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGT  >  SRR4302535.2685299/1‑94 (MQ=60)
                                                                                             GCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGT  <  SRR4302535.3446072/94‑1 (MQ=60)
                                                                                             GCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGT  >  SRR4302535.1365685/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGT  >  NC_000913/3820445‑3820631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑