Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,869,012 T→C I116V (ATT→GTT)  b3691 ← D‑galactonate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,869,0120TC100.0% 654.2 / NA 207I116V (ATT→GTT) b3691D‑galactonate transport
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (103/104);  total (103/104)

Only 100 of 221 total aligned reads displayed.

TATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAA  >  NC_000913/3868922‑3869101
                                                                                          |                                                                                         
 ataAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACAcc                                                                                        <  2:815469/93‑1 (MQ=255)
 ataAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACAcc                                                                                        >  2:756556/1‑93 (MQ=255)
 ataAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACAcc                                                                                        <  1:3494992/93‑1 (MQ=255)
  taAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCg                                                                                       >  2:2916402/1‑93 (MQ=255)
   aaaaaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCgg                                                                                      >  1:2544434/1‑93 (MQ=255)
    aaaaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGt                                                                                     <  1:1141204/93‑1 (MQ=255)
    aaaaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGt                                                                                     <  2:3854014/93‑1 (MQ=255)
     aaaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGtt                                                                                    >  2:4684622/1‑93 (MQ=255)
      aaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTa                                                                                   >  2:1531674/1‑93 (MQ=255)
      aaCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTa                                                                                   <  2:1919023/93‑1 (MQ=255)
       aCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTCTTGGTCGGGCAAGCAGGCGCTTCGAAAACACCGGCTAt                                                                                  >  1:3969411/1‑93 (MQ=255)
       aCCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTAt                                                                                  >  2:1813220/1‑93 (MQ=255)
        ccAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGATAGCAGGCGCTTCGAAAACACCGGTTATc                                                                                 >  1:2817478/1‑93 (MQ=255)
        ccAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATc                                                                                 >  1:3398936/1‑93 (MQ=255)
          aaCGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATcgc                                                                               >  1:3815233/1‑93 (MQ=255)
               cAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCa                                                                          >  1:3556171/1‑93 (MQ=255)
                 gAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGa                                                                        >  2:2143490/1‑93 (MQ=255)
                 gAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGa                                                                        >  2:139531/1‑93 (MQ=255)
                 gAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGa                                                                        >  2:1104089/1‑93 (MQ=255)
                 gAAGCGCGTTAATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCCGTTATCGCGCGCAGa                                                                        >  2:3331830/1‑93 (MQ=255)
                   aGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAcc                                                                      <  2:1095859/93‑1 (MQ=255)
                    gcgcGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCa                                                                     >  1:1229039/1‑93 (MQ=255)
                    gcgcGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCa                                                                     >  1:2067879/1‑93 (MQ=255)
                     cgcgTTCATGTTCCGGGAACCATCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCaa                                                                    <  1:879356/93‑1 (MQ=255)
                      gcgTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAAt                                                                   >  1:45332/1‑93 (MQ=255)
                      gcgTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAAt                                                                   <  2:656487/93‑1 (MQ=255)
                         ttCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTaa                                                                >  2:2635213/1‑93 (MQ=255)
                         ttCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTaa                                                                >  1:324420/1‑93 (MQ=255)
                          tCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAAt                                                               <  2:1365735/93‑1 (MQ=255)
                           cATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATg                                                              >  2:1333494/1‑93 (MQ=255)
                           cATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATg                                                              <  1:4019661/93‑1 (MQ=255)
                           cATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATg                                                              <  1:4104940/93‑1 (MQ=255)
                             tGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGAc                                                            >  2:2651913/1‑93 (MQ=255)
                               ttCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCATTTAATGCCAt                                                          >  2:4646801/1‑93 (MQ=255)
                               ttCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACAt                                                          <  1:2307791/93‑1 (MQ=255)
                               ttCCCGGAACCAGCTGCCCACCATCGGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGGCCAATTAATGACAt                                                          <  2:3667983/93‑1 (MQ=255)
                                tCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACAtt                                                         >  1:4248078/1‑93 (MQ=255)
                                  cGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTaa                                                       <  2:1120373/93‑1 (MQ=255)
                                  cGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTaa                                                       <  2:935015/93‑1 (MQ=255)
                                  cGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTaa                                                       >  1:543761/1‑93 (MQ=255)
                                   gggAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAg                                                      >  2:1015895/1‑93 (MQ=255)
                                    ggAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGc                                                     <  1:4236966/93‑1 (MQ=255)
                                    ggAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGc                                                     <  1:2971997/93‑1 (MQ=255)
                                      aaCCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGccc                                                   >  2:2242945/1‑93 (MQ=255)
                                       aCCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAGCAATTAATGACATTAAGCCCg                                                  >  1:1428854/1‑93 (MQ=255)
                                       aCCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCg                                                  >  1:778816/1‑93 (MQ=255)
                                        ccAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGt                                                 >  2:234582/1‑93 (MQ=255)
                                        ccAGCTGGTCACCATCAGGTAATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGt                                                 <  2:309656/93‑1 (MQ=255)
                                              ggTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCaaa                                           >  1:3820138/1‑93 (MQ=255)
                                              ggTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCaaa                                           <  2:3171436/93‑1 (MQ=255)
                                               gTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAg                                          >  1:1022553/1‑93 (MQ=255)
                                                tCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGc                                         <  2:1166414/93‑1 (MQ=255)
                                                tCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGc                                         <  1:2458116/93‑1 (MQ=255)
                                                 cACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGcc                                        <  1:4534476/93‑1 (MQ=255)
                                                  aCCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGccc                                       <  2:3247052/93‑1 (MQ=255)
                                                  aCCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGccc                                       <  2:3714512/93‑1 (MQ=255)
                                                  aCCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGccc                                       >  2:260041/1‑93 (MQ=255)
                                                   ccATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCt                                      >  1:1073942/1‑93 (MQ=255)
                                                    cATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTg                                     <  1:2113486/93‑1 (MQ=255)
                                                    cATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTg                                     >  1:1003899/1‑93 (MQ=255)
                                                    cATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTg                                     <  1:3606070/93‑1 (MQ=255)
                                                       ccGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGaa                                  >  1:2878992/1‑93 (MQ=255)
                                                       ccGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGaa                                  >  2:835185/1‑93 (MQ=255)
                                                        cGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAAt                                 >  1:3158682/1‑93 (MQ=255)
                                                         ggTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATa                                <  2:4194454/93‑1 (MQ=255)
                                                         ggTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATa                                >  1:3011000/1‑93 (MQ=255)
                                                          gTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATaa                               <  1:1785329/93‑1 (MQ=255)
                                                           ttATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATaaa                              <  2:2500010/93‑1 (MQ=255)
                                                           ttATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATaaa                              <  1:962592/93‑1 (MQ=255)
                                                             aTTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGt                            <  1:3683888/93‑1 (MQ=255)
                                                              ttGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTc                           >  2:3614197/1‑93 (MQ=255)
                                                              ttGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTc                           >  1:1282346/1‑93 (MQ=255)
                                                                ggTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGc                         <  1:967593/93‑1 (MQ=255)
                                                                   cGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCAc                      >  2:3375476/1‑93 (MQ=255)
                                                                   cGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCAc                      <  2:2282205/93‑1 (MQ=255)
                                                                   cGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCAc                      <  2:1426469/93‑1 (MQ=255)
                                                                    gggAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACt                     >  1:4303465/1‑93 (MQ=255)
                                                                     ggAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTg                    >  1:3352239/1‑93 (MQ=255)
                                                                      gAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGa                   <  1:2162011/93‑1 (MQ=255)
                                                                      gAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGa                   >  1:3195732/1‑93 (MQ=255)
                                                                      gAAAGCAGCCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGa                   <  2:4311231/93‑1 (MQ=255)
                                                                         aGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCa                <  1:1450489/93‑1 (MQ=255)
                                                                          gCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAg               <  1:1966621/93‑1 (MQ=255)
                                                                            aGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGcc             <  1:4492032/93‑1 (MQ=255)
                                                                            aGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGcc             >  1:2444336/1‑93 (MQ=255)
                                                                            aGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGcc             <  2:488364/93‑1 (MQ=255)
                                                                             ggCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCa            >  2:2798622/1‑93 (MQ=255)
                                                                             ggCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCa            >  2:242714/1‑93 (MQ=255)
                                                                              gcgcTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCaa           <  1:2423005/93‑1 (MQ=255)
                                                                              gcgcTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCAGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCaa           >  2:2206396/1‑93 (MQ=255)
                                                                                gcTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAat         >  1:1856314/1‑93 (MQ=255)
                                                                                gcTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAat         <  2:444264/93‑1 (MQ=255)
                                                                                gcTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAat         >  2:536379/1‑93 (MQ=255)
                                                                                 cTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAata        >  1:1903656/1‑93 (MQ=255)
                                                                                  ttCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAatat       <  1:656551/93‑1 (MQ=255)
                                                                                  ttCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAatat       >  2:3968039/1‑93 (MQ=255)
                                                                                   tCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATc      <  1:51235/93‑1 (MQ=255)
                                                                                   tCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATc      <  1:3927309/93‑1 (MQ=255)
                                                                                     gAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGc    >  2:130447/1‑93 (MQ=255)
                                                                                       aaaCACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCaa  <  1:366194/93‑1 (MQ=255)
                                                                                          |                                                                                         
TATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAA  >  NC_000913/3868922‑3869101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAA  >  NC_000913/3868919‑3869105
                                                                                             |                                                                                             
ACGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAAC                                                                                               <  SRR4302535.4137828/94‑1 (MQ=60)
ACGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAAC                                                                                               <  SRR4302535.4484850/94‑1 (MQ=60)
 CGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACA                                                                                              >  SRR4302535.3912034/1‑94 (MQ=60)
 CGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACA                                                                                              >  SRR4302535.1129584/1‑94 (MQ=60)
 CGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACA                                                                                              <  SRR4302535.4347366/94‑1 (MQ=60)
  GTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGCAAACAC                                                                                             >  SRR4302535.2802560/1‑94 (MQ=60)
  GTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACAC                                                                                             >  SRR4302535.4436263/1‑94 (MQ=60)
  GTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACAC                                                                                             <  SRR4302535.1984098/94‑1 (MQ=60)
  GTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACAC                                                                                             <  SRR4302535.186416/94‑1 (MQ=60)
  GTAAAAAAACAAGCGGCAGAAGCGCGTTCATGTGCCGGGAACCAGCTGCTCACCATCC‑GTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACC                                                                                            <  SRR4302535.2795759/94‑1 (MQ=60)
    ATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCG                                                                                           <  SRR4302535.3519272/94‑1 (MQ=60)
     TAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGG                                                                                          >  SRR4302535.2562753/1‑94 (MQ=60)
     TAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGG                                                                                          <  SRR4302535.2448320/94‑1 (MQ=60)
     TAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGG                                                                                          <  SRR4302535.2123161/94‑1 (MQ=60)
      AAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGT                                                                                         <  SRR4302535.3566176/94‑1 (MQ=60)
       AAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTT                                                                                        >  SRR4302535.3817474/1‑94 (MQ=60)
       AAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTT                                                                                        <  SRR4302535.1149337/94‑1 (MQ=60)
        AAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTA                                                                                       <  SRR4302535.263915/94‑1 (MQ=60)
        AAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTA                                                                                       <  SRR4302535.2465059/94‑1 (MQ=60)
         AACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTCTTGGTCGGGCAAGCAGGCGCTTCGAAAACACCGGCTAT                                                                                      >  SRR4302535.3996523/1‑94 (MQ=60)
          ACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGATAGCAGGCGCTTCGAAAACACCGGTTATC                                                                                     >  SRR4302535.2837489/1‑94 (MQ=60)
          ACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATC                                                                                     >  SRR4302535.3422586/1‑94 (MQ=60)
            CAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGC                                                                                   >  SRR4302535.3841557/1‑94 (MQ=60)
            CAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACAATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGC                                                                                   >  SRR4302535.3329221/1‑94 (MQ=60)
             AACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCG                                                                                  >  SRR4302535.592428/1‑94 (MQ=60)
                 GCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCA                                                                              >  SRR4302535.3580836/1‑94 (MQ=60)
                 GCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCA                                                                              >  SRR4302535.3709286/1‑94 (MQ=60)
                   AGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGC                                                                            >  SRR4302535.1217875/1‑94 (MQ=60)
                     AAGCGCGTTCATGTTCCGGGATCCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACC                                                                          <  SRR4302535.4272363/94‑1 (MQ=60)
                     AAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACC                                                                          >  SRR4302535.4096334/1‑94 (MQ=60)
                      AGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCA                                                                         >  SRR4302535.1237820/1‑94 (MQ=60)
                      AGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCA                                                                         >  SRR4302535.2082783/1‑94 (MQ=60)
                        CGCGTTCATGTTCCGGGAACCATCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAAT                                                                       <  SRR4302535.885584/94‑1 (MQ=60)
                        CGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAAT                                                                       >  SRR4302535.45675/1‑94 (MQ=60)
                         GCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATT                                                                      >  SRR4302535.61456/1‑94 (MQ=60)
                         GCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATT                                                                      <  SRR4302535.4047262/94‑1 (MQ=60)
                           GTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAGAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAA                                                                    >  SRR4302535.2935148/1‑94 (MQ=60)
                           GTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAA                                                                    <  SRR4302535.1442021/94‑1 (MQ=60)
                           GTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAA                                                                    <  SRR4302535.3394814/94‑1 (MQ=60)
                           GTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAA                                                                    <  SRR4302535.3458334/94‑1 (MQ=60)
                           GTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAA                                                                    >  SRR4302535.326663/1‑94 (MQ=60)
                             TCATGTTCCGGGAACCAGCTGGTCACCATCCGGGTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATG                                                                  >  SRR4302535.238249/1‑94 (MQ=60)
                              CATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGA                                                                 <  SRR4302535.4133044/94‑1 (MQ=60)
                              CATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGA                                                                 <  SRR4302535.4047165/94‑1 (MQ=60)
                                TGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACA                                                               <  SRR4302535.3952992/94‑1 (MQ=60)
                                  TTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATT                                                             <  SRR4302535.2324301/94‑1 (MQ=60)
                                  TTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATT                                                             >  SRR4302535.4277171/1‑94 (MQ=60)
                                    CCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAA                                                           <  SRR4302535.4565319/94‑1 (MQ=60)
                                    CCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAA                                                           >  SRR4302535.547563/1‑94 (MQ=60)
                                    CCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAA                                                           <  SRR4302535.3651475/94‑1 (MQ=60)
                                      GGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGC                                                         <  SRR4302535.1943529/94‑1 (MQ=60)
                                      GGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGC                                                         >  SRR4302535.4191167/1‑94 (MQ=60)
                                       GGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCC                                                        <  SRR4302535.2992859/94‑1 (MQ=60)
                                       GGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCC                                                        <  SRR4302535.4265962/94‑1 (MQ=60)
                                        GAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCC                                                       <  SRR4302535.2150187/94‑1 (MQ=60)
                                         AACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGAGCAATTAATGACATTAAGCCCG                                                      >  SRR4302535.1439047/1‑94 (MQ=60)
                                         AACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCG                                                      >  SRR4302535.784287/1‑94 (MQ=60)
                                           CCAGCTGGTCGCCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTG                                                    <  SRR4302535.188773/94‑1 (MQ=60)
                                            CAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGG                                                   <  SRR4302535.2500125/94‑1 (MQ=60)
                                            CAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGG                                                   >  SRR4302535.128251/1‑94 (MQ=60)
                                             AGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGC                                                  >  SRR4302535.4329409/1‑94 (MQ=60)
                                             AGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGC                                                  >  SRR4302535.1828651/1‑94 (MQ=60)
                                               CTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAA                                                >  SRR4302535.1245820/1‑94 (MQ=60)
                                                TGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAA                                               >  SRR4302535.3846495/1‑94 (MQ=60)
                                                 TGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAG                                              >  SRR4302535.1029817/1‑94 (MQ=60)
                                                  GTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGC                                             <  SRR4302535.2851906/94‑1 (MQ=60)
                                                   TCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCC                                            <  SRR4302535.2475843/94‑1 (MQ=60)
                                                   TCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCC                                            <  SRR4302535.4536319/94‑1 (MQ=60)
                                                    CACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCC                                           >  SRR4302535.22162/1‑94 (MQ=60)
                                                    CACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCC                                           <  SRR4302535.4565506/94‑1 (MQ=60)
                                                     ACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCT                                          >  SRR4302535.1706716/1‑94 (MQ=60)
                                                     ACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCT                                          <  SRR4302535.971658/94‑1 (MQ=60)
                                                     ACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCT                                          >  SRR4302535.1081579/1‑94 (MQ=60)
                                                      CCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTG                                         >  SRR4302535.1011026/1‑94 (MQ=60)
                                                       CATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGG                                        <  SRR4302535.3631086/94‑1 (MQ=60)
                                                       CATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGG                                        <  SRR4302535.2128740/94‑1 (MQ=60)
                                                       CATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGG                                        <  SRR4302535.835878/94‑1 (MQ=60)
                                                        ATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGA                                       >  SRR4302535.3475118/1‑94 (MQ=60)
                                                         TCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAA                                      >  SRR4302535.2899376/1‑94 (MQ=60)
                                                          CCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAAT                                     <  SRR4302535.1014044/94‑1 (MQ=60)
                                                          CCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAAT                                     >  SRR4302535.3180603/1‑94 (MQ=60)
                                                           CGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATA                                    >  SRR4302535.3032094/1‑94 (MQ=60)
                                                            GGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAA                                   <  SRR4302535.384434/94‑1 (MQ=60)
                                                             GTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAA                                  <  SRR4302535.1798249/94‑1 (MQ=60)
                                                             GTTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAA                                  >  SRR4302535.3099843/1‑94 (MQ=60)
                                                              TTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAG                                 <  SRR4302535.969430/94‑1 (MQ=60)
                                                              TTATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAG                                 <  SRR4302535.4003135/94‑1 (MQ=60)
                                                                ATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTC                               <  SRR4302535.3709377/94‑1 (MQ=60)
                                                                ATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTC                               >  SRR4302535.1291494/1‑94 (MQ=60)
                                                                ATTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTC                               >  SRR4302535.3141736/1‑94 (MQ=60)
                                                                 TTGGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCG                              <  SRR4302535.4664001/94‑1 (MQ=60)
                                                                   GGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCC                            <  SRR4302535.974469/94‑1 (MQ=60)
                                                                   GGTCGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCC                            >  SRR4302535.1889365/1‑94 (MQ=60)
                                                                      CGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACT                         <  SRR4302535.939656/94‑1 (MQ=60)
                                                                      CGGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACT                         >  SRR4302535.4332908/1‑94 (MQ=60)
                                                                       GGGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTG                        >  SRR4302535.3375586/1‑94 (MQ=60)
                                                                        GGAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGA                       >  SRR4302535.3217847/1‑94 (MQ=60)
                                                                         GAAAGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGAC                      <  SRR4302535.2177528/94‑1 (MQ=60)
                                                                            AGCAGGCTCTTCGAAAACACCGGTTATCGCTCGCAGACCAATTAATGACATTAAGCCCGGGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAG                   <  SRR4302535.2245261/94‑1 (MQ=60)
                                                                            AGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAG                   >  SRR4302535.3598074/1‑94 (MQ=60)
                                                                            AGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAG                   <  SRR4302535.1384140/94‑1 (MQ=60)
                                                                            AGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAG                   >  SRR4302535.685366/1‑94 (MQ=60)
                                                                            AGCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAG                   <  SRR4302535.1460865/94‑1 (MQ=60)
                                                                             GCAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGC                  <  SRR4302535.1980796/94‑1 (MQ=60)
                                                                              CAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCC                 >  SRR4302535.322226/1‑94 (MQ=60)
                                                                              CAGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCC                 >  SRR4302535.2461945/1‑94 (MQ=60)
                                                                               AGGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCA                <  SRR4302535.4522867/94‑1 (MQ=60)
                                                                                GGCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAA               >  SRR4302535.3189217/1‑94 (MQ=60)
                                                                                 GCGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAA              <  SRR4302535.2440461/94‑1 (MQ=60)
                                                                                  GGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAAT             <  SRR4302535.517617/94‑1 (MQ=60)
                                                                                  CGCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAAT             >  SRR4302535.1869684/1‑94 (MQ=60)
                                                                                   GCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATA            <  SRR4302535.3735209/94‑1 (MQ=60)
                                                                                   GCTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATA            >  SRR4302535.1917357/1‑94 (MQ=60)
                                                                                    CTTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATTACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATAT           <  SRR4302535.3979686/94‑1 (MQ=60)
                                                                                     TTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATTACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATC          >  SRR4302535.2044198/1‑94 (MQ=60)
                                                                                     TTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATC          <  SRR4302535.661132/94‑1 (MQ=60)
                                                                                     TTCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATC          >  SRR4302535.1081288/1‑94 (MQ=60)
                                                                                      TCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCG         <  SRR4302535.3954137/94‑1 (MQ=60)
                                                                                      TCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCG         <  SRR4302535.51613/94‑1 (MQ=60)
                                                                                      TCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCG         <  SRR4302535.435759/94‑1 (MQ=60)
                                                                                      TCGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCG         <  SRR4302535.2999073/94‑1 (MQ=60)
                                                                                       CGAAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAA‑CCCTGGAAaaaaagtgcccacgaacaacccaaaaacgca       >  SRR4302535.2571300/1‑63 (MQ=60)
                                                                                         AAAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAA      >  SRR4302535.111822/1‑94 (MQ=60)
                                                                                          AAACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAAT     <  SRR4302535.368715/94‑1 (MQ=60)
                                                                                           AACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATA    >  SRR4302535.4339086/1‑94 (MQ=60)
                                                                                           AACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATA    <  SRR4302535.4241483/94‑1 (MQ=60)
                                                                                            ACACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAA   <  SRR4302535.1992439/94‑1 (MQ=60)
                                                                                             CACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAA  <  SRR4302535.4096624/94‑1 (MQ=60)
                                                                                             |                                                                                             
ACGTATAAAAACCAACGGCAGAAGCGCGTTCATGTTCCGGGAACCAGCTGGTCACCATCCGGTTATTGGTCGGGAAAGCAGGCGCTTCGAAAATACCGGTTATCGCGCGCAGACCAATTAATGACATTAAGCCCGTGGCAAAGCCCTGGAATAAAGTCGCCACTGACCAGCCAAATATCGCAATAAA  >  NC_000913/3868919‑3869105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑