Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,631 Δ1 bp coding (610/1764 nt) dgoA ← 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,6300C.100.0% 1134.8 / NA 247coding (611/1764 nt)dgoA2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (129/118);  total (129/118)

Only 100 of 267 total aligned reads displayed.

CCCAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCACCTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA  >  NC_000913/3870538‑3870718
                                                                                            |                                                                                        
cccAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCAc                                                                                          <  1:668356/93‑1 (MQ=255)
cccAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCAc                                                                                          <  1:3454442/93‑1 (MQ=255)
cccAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCAc                                                                                          <  1:3743442/93‑1 (MQ=255)
cccAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCAc                                                                                          <  2:2020054/93‑1 (MQ=255)
  cAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCActg                                                                                        <  2:302329/93‑3 (MQ=255)
  cAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCActg                                                                                        >  2:2326659/1‑91 (MQ=255)
   aaCCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCActgc                                                                                       >  2:3599883/1‑90 (MQ=255)
    aCCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCActgcc                                                                                      <  2:1377126/93‑5 (MQ=255)
     ccGCCCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGCTACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCct                                                                                    >  2:4641516/1‑93 (MQ=255)
      cGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCctc                                                                                   >  2:3052790/1‑93 (MQ=255)
      cGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCctc                                                                                   <  1:4531548/93‑1 (MQ=255)
         ccACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCg                                                                                <  2:363556/93‑1 (MQ=255)
          cACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGa                                                                               >  2:1604168/1‑93 (MQ=255)
          cACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGa                                                                               <  2:3987931/93‑1 (MQ=255)
          cACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGa                                                                               <  1:1394200/93‑1 (MQ=255)
            cGCCTTCATCGGTTTCAATTTTCAGGAGCATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATa                                                                             >  2:3152254/1‑93 (MQ=255)
            cGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATa                                                                             <  1:4286330/93‑1 (MQ=255)
            cGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATa                                                                             <  2:4388373/93‑1 (MQ=255)
             gCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATAc                                                                            <  1:2995765/93‑1 (MQ=255)
              ccTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACg                                                                           >  1:3636896/1‑93 (MQ=255)
               cTTCATCGGTTTCAATTTTCAGGAACATGCATCGGGGAGGTTAATGATACGTGGTAACTTTGGTGATTTGTAATCCA‑CTGCCTCTCGATACGc                                                                          >  2:89624/1‑93 (MQ=255)
               cTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGc                                                                          >  2:210420/1‑93 (MQ=255)
                 tCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCt                                                                        <  2:3798314/93‑1 (MQ=255)
                   aTCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTa                                                                      >  2:1361196/1‑93 (MQ=255)
                    tCGGTTTTAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTTATTTTCATTGCA‑CTGCCTCTCGATACGCCTTaa                                                                     <  1:2767759/93‑1 (MQ=255)
                    tCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTaa                                                                     >  2:2358244/1‑93 (MQ=255)
                    tCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTaa                                                                     <  1:331009/93‑1 (MQ=255)
                     cGGTTTCAATTTTCAGGACCATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAAc                                                                    <  1:842313/93‑1 (MQ=255)
                      ggTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACa                                                                   >  2:2457553/1‑93 (MQ=255)
                       gTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACaa                                                                  >  1:1559739/1‑93 (MQ=255)
                        tttCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACaaa                                                                 >  1:2299077/1‑93 (MQ=255)
                        tttCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACaaa                                                                 <  2:2949983/93‑1 (MQ=255)
                         ttCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAt                                                                <  2:3867879/93‑1 (MQ=255)
                          tCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAtg                                                               >  2:1144332/1‑93 (MQ=255)
                          tCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAtg                                                               <  1:1986254/93‑1 (MQ=255)
                          tCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAtg                                                               <  2:2749345/93‑1 (MQ=255)
                           cAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAtgc                                                              >  1:1642522/1‑93 (MQ=255)
                            aaTTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAtgct                                                             >  2:72415/1‑93 (MQ=255)
                             aTTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAtgctg                                                            >  1:3880213/1‑93 (MQ=255)
                              ttttCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAtgctgc                                                           <  2:2429926/93‑1 (MQ=255)
                                 tCAGGAACATCCAGCGGGGAGGTAAACGATACGTTGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAtgctgctgc                                                        <  2:4203524/93‑1 (MQ=255)
                                   aGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGctgctgcct                                                      <  1:403360/93‑1 (MQ=255)
                                   aGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGctgctgcct                                                      <  1:2538483/93‑1 (MQ=255)
                                    ggAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGctgctgcctg                                                     >  1:733235/1‑93 (MQ=255)
                                      aaCATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGctgctgcctgct                                                   <  2:2883133/93‑1 (MQ=255)
                                      aaCATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGctgctgcctgct                                                   >  2:1411791/1‑93 (MQ=255)
                                       aCATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGctgctgcctgctg                                                  >  1:1008981/1‑93 (MQ=255)
                                          tCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTgcgc                                               >  2:4326806/1‑93 (MQ=255)
                                           ccAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGcgcg                                              <  1:3277502/93‑1 (MQ=255)
                                            cAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCgg                                             >  1:996053/1‑93 (MQ=255)
                                               cGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTgc                                          <  1:1301086/93‑1 (MQ=255)
                                               cGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTgc                                          <  2:628672/93‑1 (MQ=255)
                                                 gggAGGTAAGCGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTgcgc                                        <  1:1875212/93‑1 (MQ=255)
                                                      gTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTAc                                   <  1:2267354/93‑1 (MQ=255)
                                                      gTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTAc                                   <  2:451055/93‑1 (MQ=255)
                                                      gTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTAc                                   <  1:2517028/93‑1 (MQ=255)
                                                         aaCGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGa                                <  2:2392856/93‑1 (MQ=255)
                                                         aaCGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGa                                <  2:4633522/93‑1 (MQ=255)
                                                          aCGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGAt                               <  1:427078/93‑1 (MQ=255)
                                                          aCGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGAt                               >  1:4195659/1‑93 (MQ=255)
                                                          aCGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGAt                               <  2:783852/93‑1 (MQ=255)
                                                           cGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGAtt                              <  1:941008/93‑1 (MQ=255)
                                                           cGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGAtt                              <  1:2951707/93‑1 (MQ=255)
                                                             aTACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGc                            >  1:2891387/1‑93 (MQ=255)
                                                             aTACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGc                            >  2:4383516/1‑93 (MQ=255)
                                                             aTACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGc                            >  1:3962640/1‑93 (MQ=255)
                                                             aTACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGc                            >  1:4096367/1‑93 (MQ=255)
                                                              tACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGcc                           >  2:3916455/1‑93 (MQ=255)
                                                              tACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGcc                           <  2:4074948/93‑1 (MQ=255)
                                                              tACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGcc                           >  1:1101265/1‑93 (MQ=255)
                                                              tACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGcc                           <  1:4587492/93‑1 (MQ=255)
                                                                cGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCg                         >  1:1961632/1‑93 (MQ=255)
                                                                 gTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCgg                        <  1:1749564/93‑1 (MQ=255)
                                                                 gTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCgg                        <  1:3061274/93‑1 (MQ=255)
                                                                  tGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGgc                       >  2:936930/1‑93 (MQ=255)
                                                                  tGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGgc                       <  2:2511259/93‑1 (MQ=255)
                                                                  tGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGgc                       >  1:620091/1‑93 (MQ=255)
                                                                  tGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGgc                       >  1:3875645/1‑93 (MQ=255)
                                                                    ggaaTTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGgcgc                     >  2:3733028/3‑93 (MQ=255)
                                                                        ttttGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATa                 <  1:3621730/93‑1 (MQ=255)
                                                                          ttGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATaga               <  2:849134/93‑1 (MQ=255)
                                                                          ttGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATaga               <  1:2847538/93‑1 (MQ=255)
                                                                          ttGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATaga               <  2:1921445/93‑1 (MQ=255)
                                                                          ttGGTGATTTTCATTACA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATaga               <  2:667364/93‑1 (MQ=255)
                                                                           tGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATagag              <  2:2858717/93‑1 (MQ=255)
                                                                            ggTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAgaga             >  2:3315932/1‑93 (MQ=255)
                                                                             gTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGAt            <  1:2969367/93‑1 (MQ=255)
                                                                               gATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCg          >  2:2974724/1‑93 (MQ=255)
                                                                                aTTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGc         >  1:4336686/1‑93 (MQ=255)
                                                                                aTTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGc         <  1:1269500/93‑1 (MQ=255)
                                                                                 ttttCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCt        <  2:3451565/93‑1 (MQ=255)
                                                                                   ttCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGc      >  2:2644951/1‑93 (MQ=255)
                                                                                   ttCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGc      >  1:1469746/1‑93 (MQ=255)
                                                                                     cATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCt    >  2:4023535/1‑93 (MQ=255)
                                                                                      aTTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTa   >  2:2238304/1‑93 (MQ=255)
                                                                                      aTTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTa   >  1:2436100/1‑93 (MQ=255)
                                                                                      aTTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTa   >  2:3294398/1‑93 (MQ=255)
                                                                                       ttGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTaa  <  2:4262734/93‑1 (MQ=255)
                                                                                       ttGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTaa  <  2:1802369/93‑1 (MQ=255)
                                                                                       ttGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTaa  <  2:65224/93‑1 (MQ=255)
                                                                                            |                                                                                        
CCCAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCACCTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA  >  NC_000913/3870538‑3870718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCACCTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGC  >  NC_000913/3870538‑3870724
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CCCAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCACT                                                                                               <  SRR4302535.3478454/94‑1 (MQ=60)
CCCAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCACT                                                                                               <  SRR4302535.369777/94‑1 (MQ=60)
CCCAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCACT                                                                                               <  SRR4302535.673013/94‑1 (MQ=60)
CCCAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCACT                                                                                               <  SRR4302535.3769341/94‑1 (MQ=60)
      CGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCT                                                                                        >  SRR4302535.2006264/1‑94 (MQ=60)
      CGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCT                                                                                        <  SRR4302535.4562566/94‑1 (MQ=60)
       GACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTTCA‑CTGCCTCTC                                                                                       >  SRR4302535.80007/1‑94 (MQ=60)
       GACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTC                                                                                       >  SRR4302535.951088/1‑94 (MQ=60)
       GACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTC                                                                                       <  SRR4302535.104769/94‑1 (MQ=60)
        ACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCG                                                                                      <  SRR4302535.2997754/94‑1 (MQ=60)
         CCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGA                                                                                     >  SRR4302535.1632613/1‑94 (MQ=60)
          CACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGAT                                                                                    >  SRR4302535.3311784/1‑94 (MQ=60)
          CACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGAT                                                                                    <  SRR4302535.1404157/94‑1 (MQ=60)
          CACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGAT                                                                                    >  SRR4302535.1474835/1‑94 (MQ=60)
          CACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGAT                                                                                    >  SRR4302535.3654177/1‑94 (MQ=60)
          CACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGAT                                                                                    <  SRR4302535.1963109/94‑1 (MQ=60)
            CGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATAC                                                                                  <  SRR4302535.4315653/94‑1 (MQ=60)
            CGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATAC                                                                                  >  SRR4302535.1295371/1‑94 (MQ=60)
             GCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACG                                                                                 >  SRR4302535.3662099/1‑94 (MQ=60)
             GCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACG                                                                                 <  SRR4302535.3016765/94‑1 (MQ=60)
             GCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACG                                                                                 >  SRR4302535.3983017/1‑94 (MQ=60)
              CCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGC                                                                                >  SRR4302535.2297388/1‑94 (MQ=60)
              CCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGCAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGC                                                                                >  SRR4302535.2552670/1‑94 (MQ=60)
                   ATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAA                                                                           >  SRR4302535.2592055/1‑94 (MQ=60)
                    TCGGTTTTAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTTATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAAC                                                                          <  SRR4302535.2787466/94‑1 (MQ=60)
                    TCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAAC                                                                          <  SRR4302535.333305/94‑1 (MQ=60)
                    TCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAAC                                                                          <  SRR4302535.4129527/94‑1 (MQ=60)
                    TCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAAC                                                                          <  SRR4302535.1264124/94‑1 (MQ=60)
                     CGGTTTCAATTTTCAGGACCATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACA                                                                         <  SRR4302535.848227/94‑1 (MQ=60)
                     CGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACA                                                                         <  SRR4302535.357760/94‑1 (MQ=60)
                      GGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAA                                                                        >  SRR4302535.2792158/1‑94 (MQ=60)
                      GGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAA                                                                        >  SRR4302535.1570981/1‑94 (MQ=60)
                       GTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAA                                                                       >  SRR4302535.2315525/1‑94 (MQ=60)
                        TTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAT                                                                      >  SRR4302535.4017629/1‑94 (MQ=60)
                        TTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAAT                                                                      <  SRR4302535.1992854/94‑1 (MQ=60)
                         TTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATG                                                                     >  SRR4302535.4549257/1‑94 (MQ=60)
                          TCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGC                                                                    >  SRR4302535.1654395/1‑94 (MQ=60)
                          TCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGC                                                                    <  SRR4302535.2000558/94‑1 (MQ=60)
                           CAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCT                                                                   >  SRR4302535.2070382/1‑94 (MQ=60)
                           CAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCT                                                                   <  SRR4302535.4312378/94‑1 (MQ=60)
                           CAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCT                                                                   >  SRR4302535.3711247/1‑94 (MQ=60)
                           CAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCT                                                                   <  SRR4302535.4051137/94‑1 (MQ=60)
                            AATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTG                                                                  >  SRR4302535.3906847/1‑94 (MQ=60)
                             ATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGC                                                                 >  SRR4302535.292477/1‑94 (MQ=60)
                              TTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCT                                                                <  SRR4302535.4087872/94‑1 (MQ=60)
                                   AGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTG                                                           >  SRR4302535.738387/1‑94 (MQ=60)
                                   AGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTG                                                           <  SRR4302535.406186/94‑1 (MQ=60)
                                   AGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTG                                                           <  SRR4302535.2556768/94‑1 (MQ=60)
                                    GGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGC                                                          >  SRR4302535.1885514/1‑94 (MQ=60)
                                    GGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGC                                                          >  SRR4302535.3883016/1‑94 (MQ=60)
                                     GAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCT                                                         <  SRR4302535.4365350/94‑1 (MQ=60)
                                     GAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCT                                                         <  SRR4302535.2902722/94‑1 (MQ=60)
                                      AACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTG                                                        >  SRR4302535.1016151/1‑94 (MQ=60)
                                      AACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGAATTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTG                                                        >  SRR4302535.2529338/1‑94 (MQ=60)
                                           CCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGG                                                   >  SRR4302535.1003126/1‑94 (MQ=60)
                                           CCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGG                                                   <  SRR4302535.3300270/94‑1 (MQ=60)
                                             AGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTG                                                 <  SRR4302535.646299/94‑1 (MQ=60)
                                             AGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTG                                                 <  SRR4302535.1797787/94‑1 (MQ=60)
                                             AGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTG                                                 <  SRR4302535.3041875/94‑1 (MQ=60)
                                             AGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTG                                                 <  SRR4302535.2491092/94‑1 (MQ=60)
                                               CGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCG                                               <  SRR4302535.1310364/94‑1 (MQ=60)
                                                GGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGC                                              >  SRR4302535.3904514/1‑94 (MQ=60)
                                                 GGGAGGTAAGCGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCT                                             <  SRR4302535.1888718/94‑1 (MQ=60)
                                                  GGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTC                                            <  SRR4302535.3566206/94‑1 (MQ=60)
                                                    AGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTA                                          <  SRR4302535.4630766/94‑1 (MQ=60)
                                                     GGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTAC                                         >  SRR4302535.688308/1‑94 (MQ=60)
                                                      GTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACG                                        <  SRR4302535.2283516/94‑1 (MQ=60)
                                                      GTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACG                                        <  SRR4302535.2535172/94‑1 (MQ=60)
                                                       TAAACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGG                                       >  SRR4302535.1660920/1‑94 (MQ=60)
                                                       TAAACGATACGTGGTAATTTTGGTGATTGTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGG                                       <  SRR4302535.1425840/94‑1 (MQ=60)
                                                         AACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGAT                                     >  SRR4302535.4224373/1‑94 (MQ=60)
                                                          ACGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATT                                    <  SRR4302535.430051/94‑1 (MQ=60)
                                                           CGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTG                                   >  SRR4302535.4270300/1‑94 (MQ=60)
                                                           CGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTG                                   <  SRR4302535.1286711/94‑1 (MQ=60)
                                                           CGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTG                                   <  SRR4302535.2972443/94‑1 (MQ=60)
                                                           CGATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTG                                   <  SRR4302535.947669/94‑1 (MQ=60)
                                                            GATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGC                                  >  SRR4302535.4124405/1‑94 (MQ=60)
                                                            GATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGC                                  >  SRR4302535.3989706/1‑94 (MQ=60)
                                                            GATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGC                                  >  SRR4302535.2911841/1‑94 (MQ=60)
                                                             ATACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCC                                 >  SRR4302535.1109131/1‑94 (MQ=60)
                                                              TACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCC                                <  SRR4302535.1514960/94‑1 (MQ=60)
                                                              TACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCC                                <  SRR4302535.4618729/94‑1 (MQ=60)
                                                               ACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCG                               >  SRR4302535.1975778/1‑94 (MQ=60)
                                                               ACGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCG                               <  SRR4302535.4152771/94‑1 (MQ=60)
                                                                CGTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGG                              <  SRR4302535.3450347/94‑1 (MQ=60)
                                                                 GTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGC                             <  SRR4302535.3082683/94‑1 (MQ=60)
                                                                 GTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGC                             <  SRR4302535.1762220/94‑1 (MQ=60)
                                                                 GTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGC                             >  SRR4302535.3902268/1‑94 (MQ=60)
                                                                 GTGGTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGC                             >  SRR4302535.624403/1‑94 (MQ=60)
                                                                    GTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCG                          >  SRR4302535.3950625/1‑94 (MQ=60)
                                                                    GTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCG                          <  SRR4302535.1760456/94‑1 (MQ=60)
                                                                    GTAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCG                          >  SRR4302535.1532687/1‑94 (MQ=60)
                                                                     TAATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGA                         <  SRR4302535.1167130/94‑1 (MQ=60)
                                                                      AATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGAT                        <  SRR4302535.2981552/94‑1 (MQ=60)
                                                                      AATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGAT                        >  SRR4302535.4573043/1‑94 (MQ=60)
                                                                       ATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGGTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATA                       >  SRR4302535.804620/1‑94 (MQ=60)
                                                                       ATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATA                       <  SRR4302535.4328701/94‑1 (MQ=60)
                                                                       ATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATA                       <  SRR4302535.3181263/94‑1 (MQ=60)
                                                                       ATTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATA                       >  SRR4302535.4649032/1‑94 (MQ=60)
                                                                        TTTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAG                      <  SRR4302535.3646834/94‑1 (MQ=60)
                                                                         TTTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGA                     <  SRR4302535.2808073/94‑1 (MQ=60)
                                                                          TTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAG                    >  SRR4302535.304486/1‑94 (MQ=60)
                                                                          TTGGTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAG                    <  SRR4302535.2867720/94‑1 (MQ=60)
                                                                             GTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATC                 <  SRR4302535.4439789/94‑1 (MQ=60)
                                                                             GTGATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATC                 <  SRR4302535.2990204/94‑1 (MQ=60)
                                                                              TGATTTTCATTGCA‑CTGCCTTTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCG                <  SRR4302535.1977325/94‑1 (MQ=60)
                                                                               GATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGC               >  SRR4302535.4366348/1‑94 (MQ=60)
                                                                               GATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGC               >  SRR4302535.667859/1‑94 (MQ=60)
                                                                                ATTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCT              <  SRR4302535.1278573/94‑1 (MQ=60)
                                                                                 TTTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTG             >  SRR4302535.855471/1‑94 (MQ=60)
                                                                                  TTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGC            >  SRR4302535.1480274/1‑94 (MQ=60)
                                                                                  TTTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGC            >  SRR4302535.2440383/1‑94 (MQ=60)
                                                                                   TTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCC           >  SRR4302535.326039/1‑94 (MQ=60)
                                                                                   TTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCC           >  SRR4302535.4236176/1‑94 (MQ=60)
                                                                                   TTCATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCC           >  SRR4302535.2433809/1‑94 (MQ=60)
                                                                                     CATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTA         >  SRR4302535.2453653/1‑94 (MQ=60)
                                                                                     CATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTA         >  SRR4302535.2658076/1‑94 (MQ=60)
                                                                                     CATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTA         >  SRR4302535.1349622/1‑94 (MQ=60)
                                                                                      ATTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA        >  SRR4302535.3368704/1‑94 (MQ=60)
                                                                                       TTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAG       <  SRR4302535.866853/94‑1 (MQ=60)
                                                                                       TTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAG       <  SRR4302535.2973214/94‑1 (MQ=60)
                                                                                       TTGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAG       <  SRR4302535.3806263/94‑1 (MQ=60)
                                                                                        TGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGC      >  SRR4302535.3860727/1‑94 (MQ=60)
                                                                                        TGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGC      >  SRR4302535.834389/1‑94 (MQ=60)
                                                                                        TGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGC      <  SRR4302535.2323626/94‑1 (MQ=60)
                                                                                        TGCA‑CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGC      <  SRR4302535.4296091/94‑1 (MQ=60)
                                                                                           caCTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCC    >  SRR4302535.680789/3‑94 (MQ=60)
                                                                                           caCTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCC    >  SRR4302535.656333/3‑94 (MQ=60)
                                                                                           caCTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCC    >  SRR4302535.3665958/3‑94 (MQ=60)
                                                                                            ACTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG   >  SRR4302535.1091192/1‑94 (MQ=60)
                                                                                            ACTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG   >  SRR4302535.1749467/1‑94 (MQ=60)
                                                                                             CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGGTCTCCGGATTGCCCGGCGCGATAGAGGTCGCTGCCTAAGCCGGC  >  SRR4302535.3784720/1‑94 (MQ=60)
                                                                                             CTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGC  >  SRR4302535.3362624/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCCAACCGACCACGCCTTCATCGGTTTCAATTTTCAGGAACATCCAGCGGGGAGGTAAACGATACGTGGTAATTTTGGTGATTTTCATTGCACCTGCCTCTCGATACGCCTTAACAAATGCTGCTGCCTGCTGCGCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGC  >  NC_000913/3870538‑3870724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑