Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,896,324 +G coding (698/711 nt) b3718 ← putative isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,896,3241.G100.0% 699.2 / NA 219V233A (GTG→GCG) b3718putative isomerase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (118/101);  total (118/101)

Only 100 of 236 total aligned reads displayed.

TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA  >  NC_000913/3896232‑3896410
                                                                                             |                                                                                      
ttCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCa                                                                                         >  2:652306/1‑93 (MQ=255)
ttCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCa                                                                                         >  2:3333733/1‑93 (MQ=255)
 tCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCag                                                                                        <  1:3004188/93‑2 (MQ=255)
   gTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagcg                                                                                      >  1:4603879/1‑90 (MQ=255)
   gTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagcg                                                                                      <  1:4468600/93‑4 (MQ=255)
    tAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCagcgg                                                                                     <  2:2907671/93‑5 (MQ=255)
        tATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCttt                                                                                 >  1:430361/1‑93 (MQ=255)
         aTTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTa                                                                                <  1:4650393/93‑1 (MQ=255)
         aTTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTa                                                                                <  1:863691/93‑1 (MQ=255)
          tttAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAt                                                                               <  2:2293333/93‑1 (MQ=255)
          tttAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAt                                                                               <  2:3000983/93‑1 (MQ=255)
          tttAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAt                                                                               >  1:2140808/1‑93 (MQ=255)
           ttAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATc                                                                              <  2:1897270/93‑1 (MQ=255)
            tAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCa                                                                             <  1:2652287/93‑1 (MQ=255)
             aaaTAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAg                                                                            <  2:2651562/93‑1 (MQ=255)
              aataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                                           >  1:3654913/1‑93 (MQ=255)
              aataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGc                                                                           <  1:1630704/93‑1 (MQ=255)
               ataatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCg                                                                          >  1:4019835/1‑93 (MQ=255)
                taatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGa                                                                         >  2:1004094/1‑93 (MQ=255)
                taatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGa                                                                         <  2:1305687/93‑1 (MQ=255)
                 aatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAt                                                                        <  1:2083037/93‑1 (MQ=255)
                 aatGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAt                                                                        >  1:94110/1‑93 (MQ=255)
                   tGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTa                                                                      >  1:3315210/1‑93 (MQ=255)
                   tGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTa                                                                      <  1:1578394/93‑1 (MQ=255)
                    gagaACAGGCCGGAGCGTAATTCACACATCCCGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCCGCTTTATCAGCGATTAc                                                                     >  2:2670185/1‑93 (MQ=255)
                     agaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAcc                                                                    >  1:3136891/1‑93 (MQ=255)
                      gaACAGGCCGGAGCGTAATTCACATATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCa                                                                   >  2:4517948/1‑93 (MQ=255)
                      gaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCa                                                                   <  2:4263148/93‑1 (MQ=255)
                      gaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCa                                                                   <  2:277442/93‑1 (MQ=255)
                      gaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCa                                                                   >  1:3461304/1‑93 (MQ=255)
                      gaACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCa                                                                   <  1:47195/93‑1 (MQ=255)
                        aCAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATc                                                                 <  1:561097/93‑1 (MQ=255)
                         cAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCa                                                                >  1:3808416/1‑93 (MQ=255)
                          aGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAg                                                               >  1:3903142/1‑93 (MQ=255)
                           ggCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGc                                                              >  1:3548178/1‑93 (MQ=255)
                              cGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGaa                                                           <  2:2699528/93‑1 (MQ=255)
                              cGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGaa                                                           <  1:4069127/93‑1 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          <  2:1685571/93‑1 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          >  1:1221570/1‑93 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          <  1:2578309/93‑1 (MQ=255)
                               ggAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAg                                                          >  2:694472/1‑93 (MQ=255)
                                gAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAgg                                                         <  2:165656/93‑1 (MQ=255)
                                  gCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAt                                                       >  1:1114083/1‑93 (MQ=255)
                                  gCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAt                                                       <  1:4394536/93‑1 (MQ=255)
                                  gCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAt                                                       >  2:572882/1‑93 (MQ=255)
                                    gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc                                                     >  2:4342700/1‑93 (MQ=255)
                                    gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc                                                     >  1:1787618/1‑93 (MQ=255)
                                    gTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc                                                     <  2:278535/93‑1 (MQ=255)
                                     tAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATACa                                                    >  2:447046/1‑93 (MQ=255)
                                       aTTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGc                                                  >  2:598834/1‑93 (MQ=255)
                                            cacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCaa                                             <  2:853177/93‑1 (MQ=255)
                                            cacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCaa                                             <  1:2300513/93‑1 (MQ=255)
                                            cacaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCaa                                             >  1:326039/1‑93 (MQ=255)
                                             acaTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAac                                            <  2:2838802/93‑1 (MQ=255)
                                              caTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAaca                                           >  1:4642189/1‑93 (MQ=255)
                                              caTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAaca                                           >  2:3256344/1‑93 (MQ=255)
                                              caTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAaca                                           >  1:2479102/1‑93 (MQ=255)
                                              caTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAaca                                           >  2:2329716/1‑93 (MQ=255)
                                               aTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAacac                                          <  2:460171/93‑1 (MQ=255)
                                               aTCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAacac                                          >  1:92258/1‑93 (MQ=255)
                                                tCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACg                                         >  1:17107/1‑93 (MQ=255)
                                                tCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACg                                         >  1:4217043/1‑93 (MQ=255)
                                                   ggCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGaa                                      >  1:137037/1‑93 (MQ=255)
                                                   ggCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGaa                                      >  1:160037/1‑93 (MQ=255)
                                                    gCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAg                                     >  2:2516556/1‑93 (MQ=255)
                                                     ccTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGc                                    >  1:63635/1‑93 (MQ=255)
                                                     ccTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGc                                    <  1:3075168/93‑1 (MQ=255)
                                                     ccTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGc                                    >  2:1975547/1‑93 (MQ=255)
                                                      cTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCt                                   >  1:1517749/1‑93 (MQ=255)
                                                      cTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCAACAGCGAAGGATGCAGCTGCAACACGGAAGCt                                   >  1:4355944/1‑93 (MQ=255)
                                                       ttATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTg                                  >  2:2118083/1‑93 (MQ=255)
                                                       ttATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTg                                  >  1:2035601/1‑93 (MQ=255)
                                                       ttATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTg                                  >  2:4074963/1‑93 (MQ=255)
                                                        tATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGCAGCTgg                                 >  2:4423755/1‑93 (MQ=255)
                                                         aTTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGa                                >  1:72766/1‑93 (MQ=255)
                                                         aTTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGa                                >  1:835855/1‑93 (MQ=255)
                                                         aTTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGa                                >  2:2899251/1‑93 (MQ=255)
                                                          tttCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGaa                               <  1:2395299/93‑1 (MQ=255)
                                                          tttCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGaa                               >  1:1488259/1‑93 (MQ=255)
                                                           ttCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAAc                              <  1:2909021/93‑1 (MQ=255)
                                                           ttCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAAc                              >  1:4533142/1‑93 (MQ=255)
                                                              ttAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATc                           <  1:3737893/93‑1 (MQ=255)
                                                                 aGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTc                        <  1:2045604/93‑1 (MQ=255)
                                                                  gCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCg                       >  1:3465052/1‑93 (MQ=255)
                                                                  gCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCg                       >  1:504313/1‑93 (MQ=255)
                                                                    ttAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGt                     <  2:4037010/93‑1 (MQ=255)
                                                                       aCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAc                  <  1:4169460/93‑1 (MQ=255)
                                                                       aCCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAc                  >  1:1925937/1‑93 (MQ=255)
                                                                        ccGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAcc                 <  2:662351/93‑1 (MQ=255)
                                                                          gAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCgg               <  2:3870236/93‑1 (MQ=255)
                                                                           aGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGa              <  1:268287/93‑1 (MQ=255)
                                                                           aGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGa              >  2:3606423/1‑93 (MQ=255)
                                                                                  aTTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAACTTGCa       >  1:3390136/1‑93 (MQ=255)
                                                                                   ttCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAg      >  2:2144611/1‑93 (MQ=255)
                                                                                   ttCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAg      >  2:4293735/1‑93 (MQ=255)
                                                                                   ttCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAg      >  1:3726581/1‑93 (MQ=255)
                                                                                      tGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAc   >  2:3762163/1‑93 (MQ=255)
                                                                                      tGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAc   <  2:2057571/93‑1 (MQ=255)
                                                                                      tGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAc   <  1:1287427/93‑1 (MQ=255)
                                                                                       gCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACa  <  2:804650/93‑1 (MQ=255)
                                                                                             |                                                                                      
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA  >  NC_000913/3896232‑3896410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCA  >  NC_000913/3896232‑3896417
                                                                                            |                                                                                              
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑G                                                                                              <  SRR4302535.929675/94‑1 (MQ=60)
 TCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgc                                                                                              <  SRR4302535.3025246/94‑3 (MQ=60)
 TCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgc                                                                                              <  SRR4302535.4668051/94‑3 (MQ=60)
  CGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgcg                                                                                             >  SRR4302535.4635179/1‑91 (MQ=60)
   GTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAgcgc                                                                                            >  SRR4302535.2624429/1‑90 (MQ=60)
   GTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGG                                                                                            <  SRR4302535.1703430/94‑1 (MQ=60)
   GTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGG                                                                                            <  SRR4302535.4499262/94‑1 (MQ=60)
      GTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTT                                                                                         <  SRR4302535.2167295/94‑1 (MQ=60)
       TTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTT                                                                                        >  SRR4302535.433355/1‑94 (MQ=60)
         ATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAT                                                                                      <  SRR4302535.4681885/94‑1 (MQ=60)
         ATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAT                                                                                      <  SRR4302535.869793/94‑1 (MQ=60)
         ATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAT                                                                                      <  SRR4302535.242819/94‑1 (MQ=60)
         ATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTAT                                                                                      >  SRR4302535.2156219/1‑94 (MQ=60)
          TTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATC                                                                                     >  SRR4302535.2358763/1‑94 (MQ=60)
           TTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCA                                                                                    >  SRR4302535.894547/1‑94 (MQ=60)
            TAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAG                                                                                   <  SRR4302535.2671287/94‑1 (MQ=60)
            TAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAG                                                                                   <  SRR4302535.2671288/94‑1 (MQ=60)
             AAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGC                                                                                  >  SRR4302535.488437/1‑94 (MQ=60)
             AAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGC                                                                                  >  SRR4302535.737554/1‑94 (MQ=60)
             AAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGC                                                                                  >  SRR4302535.3680214/1‑94 (MQ=60)
              AATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCG                                                                                 <  SRR4302535.1642478/94‑1 (MQ=60)
              AATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCG                                                                                 >  SRR4302535.4047341/1‑94 (MQ=60)
              AATAATGAAGACAGGCCGGAGCGTAATTCACCCATCCGGCCTTATTTCTTAAGCTTAACCGGATGCTAATTCTGCGGCAGCGGCTTTATCAGCG                                                                                 <  SRR4302535.4613556/94‑1 (MQ=60)
               ATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTCAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGT                                                                                >  SRR4302535.381112/1‑94 (MQ=60)
               ATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGA                                                                                >  SRR4302535.1158397/1‑94 (MQ=60)
                TAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAT                                                                               <  SRR4302535.3082728/94‑1 (MQ=60)
                TAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGAT                                                                               >  SRR4302535.94780/1‑94 (MQ=60)
                 AATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATT                                                                              <  SRR4302535.2098039/94‑1 (MQ=60)
                 AATGAGAACAGGCCGGAGCGTAATTCACACATCAGGCCTTATTTCTTAAGCCTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGACT                                                                              >  SRR4302535.3968776/1‑94 (MQ=60)
                  ATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTA                                                                             >  SRR4302535.3547738/1‑94 (MQ=60)
                  ATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTA                                                                             >  SRR4302535.3338276/1‑94 (MQ=60)
                   TGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAC                                                                            <  SRR4302535.3486956/94‑1 (MQ=60)
                   TGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAC                                                                            <  SRR4302535.1589789/94‑1 (MQ=60)
                    GAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACC                                                                           >  SRR4302535.3158722/1‑94 (MQ=60)
                    GAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACC                                                                           >  SRR4302535.3839933/1‑94 (MQ=60)
                     AGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCA                                                                          >  SRR4302535.3485370/1‑94 (MQ=60)
                      GAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCAT                                                                         <  SRR4302535.47550/94‑1 (MQ=60)
                       AACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATC                                                                        >  SRR4302535.4396325/1‑94 (MQ=60)
                        ACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCA                                                                       <  SRR4302535.565020/94‑1 (MQ=60)
                        ACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCA                                                                       >  SRR4302535.3834686/1‑94 (MQ=60)
                         CAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAG                                                                      >  SRR4302535.3929880/1‑94 (MQ=60)
                         CAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAG                                                                      <  SRR4302535.1509939/94‑1 (MQ=60)
                          AGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGC                                                                     >  SRR4302535.3572810/1‑94 (MQ=60)
                          AGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGC                                                                     >  SRR4302535.1610393/1‑94 (MQ=60)
                             CCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTCCCATCgacgag                                                                  >  SRR4302535.4110617/1‑88 (MQ=60)
                              CGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAG                                                                 <  SRR4302535.4096975/94‑1 (MQ=60)
                              CGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAG                                                                 >  SRR4302535.1230322/1‑94 (MQ=60)
                               GGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGG                                                                >  SRR4302535.1328475/1‑94 (MQ=60)
                               GGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGG                                                                >  SRR4302535.3209962/1‑94 (MQ=60)
                               GGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGG                                                                <  SRR4302535.2596836/94‑1 (MQ=60)
                               GGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGG                                                                <  SRR4302535.4681276/94‑1 (MQ=60)
                                GAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGA                                                               <  SRR4302535.1935506/94‑1 (MQ=60)
                                 AGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAT                                                              >  SRR4302535.1122025/1‑94 (MQ=60)
                                 AGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAT                                                              >  SRR4302535.3146107/1‑94 (MQ=60)
                                 AGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATAAGCGAAGGAT                                                              <  SRR4302535.4178628/94‑1 (MQ=60)
                                 AGCGTAATTCACACATCCGGCCTTATTTCTTAAAATTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGAT                                                              >  SRR4302535.4311768/1‑94 (MQ=60)
                                  GCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATG                                                             <  SRR4302535.4424608/94‑1 (MQ=60)
                                  GCCTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATG                                                             <  SRR4302535.47451/94‑1 (MQ=60)
                                   ctcAATTCACACATCCGGCCTTATTACTTAAGCTTAACCGAGTGCTAATTCTTCGGCAGCGGCTTTATCAGCGATTACCATCAGCTAAGGATGC                                                            <  SRR4302535.3760159/91‑1 (MQ=60)
                                   CGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGC                                                            >  SRR4302535.1800549/1‑94 (MQ=60)
                                    GTAATTCGCACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           >  SRR4302535.913401/1‑94 (MQ=60)
                                    GTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCA                                                           <  SRR4302535.1768282/94‑1 (MQ=60)
                                      AATTCACACATCCGGCCTTATTTCTTTAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGC                                                         >  SRR4302535.863834/1‑94 (MQ=60)
                                      AATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGC                                                         <  SRR4302535.640943/94‑1 (MQ=60)
                                       ATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCCTCAGCGAAGGATCCAGCT                                                        >  SRR4302535.3763253/1‑94 (MQ=60)
                                       ATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCT                                                        <  SRR4302535.1558728/94‑1 (MQ=60)
                                        TTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTG                                                       <  SRR4302535.1364147/94‑1 (MQ=60)
                                        TTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTG                                                       >  SRR4302535.4453412/1‑94 (MQ=60)
                                          CACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCA                                                     >  SRR4302535.251781/1‑94 (MQ=60)
                                          CACACATCCGGCCTTATTACTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCA                                                     <  SRR4302535.1976928/94‑1 (MQ=60)
                                           ACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAA                                                    >  SRR4302535.328300/1‑94 (MQ=60)
                                            CACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAAC                                                   <  SRR4302535.2316975/94‑1 (MQ=60)
                                            CACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAAA                                                   >  SRR4302535.1985351/1‑94 (MQ=60)
                                             ACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACA                                                  >  SRR4302535.1286664/1‑94 (MQ=60)
                                             ACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACA                                                  >  SRR4302535.2496983/1‑94 (MQ=60)
                                             ACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACA                                                  >  SRR4302535.4673655/1‑94 (MQ=60)
                                              CATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACAC                                                 <  SRR4302535.2843213/94‑1 (MQ=60)
                                              CATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACAC                                                 >  SRR4302535.92912/1‑94 (MQ=60)
                                               ATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACG                                                >  SRR4302535.17234/1‑94 (MQ=60)
                                               ATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACG                                                >  SRR4302535.4245899/1‑94 (MQ=60)
                                                  CGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAA                                             >  SRR4302535.161160/1‑94 (MQ=60)
                                                  CGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAA                                             >  SRR4302535.137997/1‑94 (MQ=60)
                                                  CGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAA                                             >  SRR4302535.978741/1‑94 (MQ=60)
                                                   GGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAG                                            <  SRR4302535.1632951/94‑1 (MQ=60)
                                                    GCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGC                                           <  SRR4302535.4058425/94‑1 (MQ=60)
                                                    GCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGC                                           >  SRR4302535.64114/1‑94 (MQ=60)
                                                     CCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCT                                          >  SRR4302535.1528682/1‑94 (MQ=60)
                                                     CCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCT                                          >  SRR4302535.52553/1‑94 (MQ=60)
                                                     CCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCT                                          >  SRR4302535.2065917/1‑94 (MQ=60)
                                                     CCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCT                                          <  SRR4302535.3096627/94‑1 (MQ=60)
                                                     CCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCAACAGCGAAGGATGCAGCTGCAACACGGAAGCT                                          >  SRR4302535.4385759/1‑94 (MQ=60)
                                                      CTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTG                                         >  SRR4302535.2050264/1‑94 (MQ=60)
                                                      CTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTG                                         >  SRR4302535.4177062/1‑94 (MQ=60)
                                                       TTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGG                                        >  SRR4302535.210090/1‑94 (MQ=60)
                                                       TTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGG                                        <  SRR4302535.1536090/94‑1 (MQ=60)
                                                       TTATCTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGG                                        <  SRR4302535.1462717/94‑1 (MQ=60)
                                                        TATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGA                                       >  SRR4302535.841718/1‑94 (MQ=60)
                                                        TATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGA                                       >  SRR4302535.73295/1‑94 (MQ=60)
                                                        TATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGA                                       >  SRR4302535.3344432/1‑94 (MQ=60)
                                                         ATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAA                                      >  SRR4302535.1498928/1‑94 (MQ=60)
                                                         ATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAA                                      >  SRR4302535.2659027/1‑94 (MQ=60)
                                                          TTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAAC                                     <  SRR4302535.2412514/94‑1 (MQ=60)
                                                          TTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAAC                                     >  SRR4302535.4564166/1‑94 (MQ=60)
                                                           TTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACA                                    <  SRR4302535.700631/94‑1 (MQ=60)
                                                           TTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACA                                    <  SRR4302535.2929578/94‑1 (MQ=60)
                                                            TCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACAT                                   >  SRR4302535.1702028/1‑94 (MQ=60)
                                                             CTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATC                                  >  SRR4302535.3562780/1‑94 (MQ=60)
                                                              TTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCT                                 <  SRR4302535.3763763/94‑1 (MQ=60)
                                                              TTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCT                                 <  SRR4302535.4607889/94‑1 (MQ=60)
                                                               TAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTT                                >  SRR4302535.2992292/1‑94 (MQ=60)
                                                                AAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTC                               <  SRR4302535.4369538/94‑1 (MQ=60)
                                                                 AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              >  SRR4302535.3489135/1‑94 (MQ=60)
                                                                 AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302535.3102743/94‑1 (MQ=60)
                                                                 AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302535.4033099/94‑1 (MQ=60)
                                                                 AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              >  SRR4302535.507838/1‑94 (MQ=60)
                                                                 AGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCG                              <  SRR4302535.2060336/94‑1 (MQ=60)
                                                                  GCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGG                             <  SRR4302535.3359553/94‑1 (MQ=60)
                                                                   CTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGT                            >  SRR4302535.619455/1‑94 (MQ=60)
                                                                   CTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGA                            >  SRR4302535.3000630/1‑94 (MQ=60)
                                                                     TAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAA                          >  SRR4302535.2344326/1‑94 (MQ=60)
                                                                     TAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAA                          >  SRR4302535.3613989/1‑94 (MQ=60)
                                                                      AACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAC                         >  SRR4302535.1939820/1‑94 (MQ=60)
                                                                      AACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAC                         >  SRR4302535.4526679/1‑94 (MQ=60)
                                                                      AACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAAC                         >  SRR4302535.4526680/1‑94 (MQ=60)
                                                                       ACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACC                        <  SRR4302535.4198011/94‑1 (MQ=60)
                                                                        CCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCG                       <  SRR4302535.503674/94‑1 (MQ=60)
                                                                         CGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGG                      >  SRR4302535.348126/1‑94 (MQ=60)
                                                                          GAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGA                     <  SRR4302535.4359256/94‑1 (MQ=60)
                                                                           AGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAC                    <  SRR4302535.270151/94‑1 (MQ=60)
                                                                             TGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCT                  <  SRR4302535.3737078/94‑1 (MQ=60)
                                                                              GCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGTAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTT                 <  SRR4302535.3330242/94‑1 (MQ=60)
                                                                              GCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGGCCTT                 <  SRR4302535.4241478/94‑1 (MQ=60)
                                                                                 AATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGAACTTGCA              >  SRR4302535.3413721/1‑94 (MQ=60)
                                                                                  ATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAG             >  SRR4302535.3752398/1‑94 (MQ=60)
                                                                                     CTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAC          <  SRR4302535.2051807/94‑1 (MQ=60)
                                                                                     CTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAAC          <  SRR4302535.2062828/94‑1 (MQ=60)
                                                                                      TGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACA         <  SRR4302535.1296603/94‑1 (MQ=60)
                                                                                       GCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACAT        <  SRR4302535.4681728/94‑1 (MQ=60)
                                                                                        CGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATT       <  SRR4302535.263311/94‑1 (MQ=60)
                                                                                         GGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTT      >  SRR4302535.4155374/1‑94 (MQ=60)
                                                                                          GCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTT     <  SRR4302535.2712630/94‑1 (MQ=60)
                                                                                            G‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCA  <  SRR4302535.1705080/94‑1 (MQ=60)
                                                                                            |                                                                                              
TTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCA‑CGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACACGGAAGCTGGAACATCTTCGGTAACCGGACCTTGCAGAACATTTTTCA  >  NC_000913/3896232‑3896417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑