Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,930,568 +G intergenic (+66/‑373) kup → / → rbsD low affinity potassium transport system/RbsD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,930,5671.G100.0% 724.5 / NA 229intergenic (+65/‑374)kup/rbsDlow affinity potassium transport system/RbsD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (94/135);  total (94/135)

Only 100 of 255 total aligned reads displayed.

GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATG  >  NC_000913/3930475‑3930655
                                                                                             |                                                                                        
 cACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑g                                                                                         <  1:3537441/93‑1 (MQ=255)
  aCCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑gg                                                                                        >  2:2712835/1‑92 (MQ=255)
    cGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggcg                                                                                      >  1:3290142/1‑90 (MQ=255)
     gAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggcga                                                                                     <  1:3954406/93‑5 (MQ=255)
        gACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGaa                                                                                   >  2:585030/1‑93 (MQ=255)
        gACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGaa                                                                                   <  2:1761620/93‑1 (MQ=255)
        gACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCCGGGGGGCAAGTTATGGTTGGCGAGaa                                                                                   >  2:3297244/1‑93 (MQ=255)
         aCGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGaaa                                                                                  <  1:1469567/93‑1 (MQ=255)
         aCGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGaaa                                                                                  <  1:1922353/93‑1 (MQ=255)
          cGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAAc                                                                                 <  2:930755/93‑1 (MQ=255)
          cGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAAc                                                                                 <  1:2362446/93‑1 (MQ=255)
             tCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGcc                                                                              >  1:2322782/1‑93 (MQ=255)
              ccATATGCCCATAACGCCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTCCTGGCGCGTGGTGGCAGGTTATGGTTGGCGAGAAACGCCa                                                                             <  2:1791084/93‑1 (MQ=255)
                 tatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAc                                                                          <  2:306234/93‑1 (MQ=255)
                 tatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAc                                                                          <  1:32964/93‑1 (MQ=255)
                    gTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTa                                                                       <  1:1127924/93‑1 (MQ=255)
                     tCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTag                                                                      <  2:3405260/93‑1 (MQ=255)
                      ccATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTaga                                                                     <  2:389616/93‑1 (MQ=255)
                       cATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaa                                                                    <  2:704910/93‑1 (MQ=255)
                       cATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaa                                                                    <  1:733087/93‑1 (MQ=255)
                       cATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaa                                                                    <  1:1453805/93‑1 (MQ=255)
                        aTAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaag                                                                   <  1:3782144/93‑1 (MQ=255)
                        aTAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaag                                                                   <  2:2443557/93‑1 (MQ=255)
                         tAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaaga                                                                  <  2:3657163/93‑1 (MQ=255)
                         tAACGTCCGGCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaaga                                                                  <  1:1056418/93‑1 (MQ=255)
                          aaCGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAgaagaa                                                                 <  1:2662258/93‑1 (MQ=255)
                          aaCGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAgaagaa                                                                 >  1:2128109/1‑93 (MQ=255)
                           aCGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGaagaag                                                                >  2:909005/1‑93 (MQ=255)
                             gtccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtt                                                              <  1:4290560/93‑1 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                             <  1:456358/93‑1 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                             <  1:4505027/93‑1 (MQ=255)
                               ccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtttt                                                            <  2:2208943/93‑1 (MQ=255)
                                cgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTc                                                           <  1:2622091/93‑1 (MQ=255)
                                 gtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTcc                                                          >  2:4346491/1‑93 (MQ=255)
                                 gtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTCATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTcc                                                          <  1:3387632/93‑1 (MQ=255)
                                     ggTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCg                                                      >  1:2771832/1‑93 (MQ=255)
                                      gTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGc                                                     <  1:30269/93‑1 (MQ=255)
                                       tACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCt                                                    >  1:1299907/1‑93 (MQ=255)
                                        aCAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTg                                                   >  2:1020897/1‑93 (MQ=255)
                                        aCAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTg                                                   >  1:786629/1‑93 (MQ=255)
                                         cAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGc                                                  <  1:3682039/93‑1 (MQ=255)
                                          aGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCg                                                 <  2:8240/93‑1 (MQ=255)
                                           gATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCgg                                                <  1:2468842/93‑1 (MQ=255)
                                           gATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCgg                                                >  1:601527/1‑93 (MQ=255)
                                            aTTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGt                                               <  2:2386393/93‑1 (MQ=255)
                                             ttGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTc                                              <  1:1154195/93‑1 (MQ=255)
                                             ttGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTc                                              <  1:235590/93‑1 (MQ=255)
                                             ttGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTc                                              <  2:4216316/93‑1 (MQ=255)
                                             ttGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTc                                              <  1:3617890/93‑1 (MQ=255)
                                                 acaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGa                                          >  1:956845/1‑93 (MQ=255)
                                                   aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAg                                        <  1:499742/93‑1 (MQ=255)
                                                   aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGCCTGGAAg                                        >  1:482697/1‑93 (MQ=255)
                                                    acTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAgg                                       >  2:3556159/1‑93 (MQ=255)
                                                     cTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGa                                      >  1:2815933/1‑93 (MQ=255)
                                                     cTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGa                                      >  1:2997015/1‑93 (MQ=255)
                                                      tGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGAt                                     <  2:1628941/93‑1 (MQ=255)
                                                       gTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGAtt                                    <  1:200441/93‑1 (MQ=255)
                                                         cGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTaa                                  <  2:376563/93‑1 (MQ=255)
                                                          gCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAg                                 >  1:3127657/1‑93 (MQ=255)
                                                          gCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAAAGCCAAACGTGGAAGAAGGTTTACACCGCTGCGGTCTGGAAGGATTAAg                                 >  1:3836490/1‑93 (MQ=255)
                                                           cccACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGt                                <  2:3323934/93‑1 (MQ=255)
                                                           cccACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGt                                <  1:1733873/93‑1 (MQ=255)
                                                           cccACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGt                                >  2:4195051/1‑93 (MQ=255)
                                                            cgaCTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGtt                               >  2:2957843/3‑93 (MQ=255)
                                                            ccACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCGCCGCTGCGGTCTGGAAGGATTAAGtt                               >  2:3792690/1‑93 (MQ=255)
                                                            ccACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGtt                               >  2:3870255/1‑93 (MQ=255)
                                                              aCTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGc                             >  1:2998837/1‑93 (MQ=255)
                                                              aCTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGc                             >  2:3724181/1‑93 (MQ=255)
                                                              aCTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGc                             >  2:1495255/1‑93 (MQ=255)
                                                               cTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGcc                            >  2:3105163/1‑93 (MQ=255)
                                                                tttCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGcgg                           >  2:1943694/1‑91 (MQ=255)
                                                                tttCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCg                           >  1:290127/1‑93 (MQ=255)
                                                                tttCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCg                           >  2:1703481/1‑93 (MQ=255)
                                                                   cTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgat                        <  2:724803/93‑1 (MQ=255)
                                                                   cTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgat                        <  2:1114888/93‑1 (MQ=255)
                                                                    tGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatg                       >  2:498332/1‑93 (MQ=255)
                                                                    tGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatg                       >  1:3081196/1‑93 (MQ=255)
                                                                     ggCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatga                      >  2:1303705/1‑93 (MQ=255)
                                                                       cgcgTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGatgatg                    <  2:3578940/93‑1 (MQ=255)
                                                                        gcgTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATgg                   <  2:3187262/93‑1 (MQ=255)
                                                                        gcgTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATgg                   <  2:111315/93‑1 (MQ=255)
                                                                          gtggtgGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGaa                 >  2:1810650/1‑93 (MQ=255)
                                                                           tggtggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGaaa                <  2:320951/93‑1 (MQ=255)
                                                                           tggtggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGaaa                >  2:562859/1‑93 (MQ=255)
                                                                           tggtggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGaaa                >  2:4662159/1‑93 (MQ=255)
                                                                             gtggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcc              <  1:4315025/93‑1 (MQ=255)
                                                                              tggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcct             >  2:4063214/1‑93 (MQ=255)
                                                                               ggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcctc            <  2:2079811/93‑1 (MQ=255)
                                                                                gCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcctcc           <  2:4587470/93‑1 (MQ=255)
                                                                                gCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcctcc           >  2:1317208/1‑93 (MQ=255)
                                                                                 cAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcctcct          <  1:666841/93‑1 (MQ=255)
                                                                                  aaGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCctt         <  1:4135261/93‑1 (MQ=255)
                                                                                  aaGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCctt         <  1:3427065/93‑1 (MQ=255)
                                                                                  aaGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCAGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCctt         <  1:1758358/93‑1 (MQ=255)
                                                                                   aGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttc        >  1:539529/1‑93 (MQ=255)
                                                                                    gTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttct       <  2:3993714/93‑1 (MQ=255)
                                                                                    gTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttct       <  1:3170290/93‑1 (MQ=255)
                                                                                    gTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttct       >  2:2763080/1‑93 (MQ=255)
                                                                                    gTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttct       >  1:4198254/1‑93 (MQ=255)
                                                                                     ttATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttctt      <  2:521219/93‑1 (MQ=255)
                                                                                             |                                                                                        
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATG  >  NC_000913/3930475‑3930655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  >  NC_000913/3930476‑3930661
                                                                                              |                                                                                             
CACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTT‑GG                                                                                              <  SRR4302535.3366316/94‑1 (MQ=60)
CACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTT‑GG                                                                                              <  SRR4302535.955223/94‑1 (MQ=60)
CACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTT‑GG                                                                                              <  SRR4302535.3561999/94‑1 (MQ=60)
 ACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTT‑Ggc                                                                                             <  SRR4302535.4596155/94‑3 (MQ=60)
  CCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTT‑Ggcg                                                                                            >  SRR4302535.791535/1‑91 (MQ=60)
  CCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTT‑Ggcg                                                                                            >  SRR4302535.3313017/1‑91 (MQ=60)
    GAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAG                                                                                           <  SRR4302535.3981402/94‑1 (MQ=60)
    GAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAG                                                                                           <  SRR4302535.4592070/94‑1 (MQ=60)
    GAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAG                                                                                           >  SRR4302535.585327/1‑94 (MQ=60)
     AAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGA                                                                                          >  SRR4302535.1908063/1‑94 (MQ=60)
     AAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTT‑Ggggaga                                                                                         >  SRR4302535.3690099/1‑88 (MQ=60)
      AGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAA                                                                                         >  SRR4302535.421344/1‑94 (MQ=60)
      AGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAA                                                                                         <  SRR4302535.4210009/94‑1 (MQ=60)
        ACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAAC                                                                                       <  SRR4302535.3474040/94‑1 (MQ=60)
        ACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAAC                                                                                       <  SRR4302535.1480094/94‑1 (MQ=60)
        ACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAAC                                                                                       <  SRR4302535.2372587/94‑1 (MQ=60)
        ACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAAC                                                                                       <  SRR4302535.1936209/94‑1 (MQ=60)
         CGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACG                                                                                      <  SRR4302535.2379413/94‑1 (MQ=60)
           CTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCC                                                                                    >  SRR4302535.2339404/1‑94 (MQ=60)
           CTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCC                                                                                    <  SRR4302535.739579/94‑1 (MQ=60)
            TCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCA                                                                                   >  SRR4302535.2177631/1‑94 (MQ=60)
              CATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAA                                                                                 <  SRR4302535.1459113/94‑1 (MQ=60)
               ATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATCGCTG‑GCGAGAAACGCCACAC                                                                                >  SRR4302535.3905159/1‑94 (MQ=60)
               ATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGAGCGTGGGGGCAAGTTAT‑GGTTGGCGGGAAAAGCCAAAC                                                                                >  SRR4302535.3025548/1‑94 (MQ=60)
                TATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACG                                                                               <  SRR4302535.33218/94‑1 (MQ=60)
                TATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACG                                                                               <  SRR4302535.3792299/94‑1 (MQ=60)
                 ATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGT                                                                              >  SRR4302535.2062626/1‑94 (MQ=60)
                 ATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGT                                                                              <  SRR4302535.4062476/94‑1 (MQ=60)
                   GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302535.1135977/94‑1 (MQ=60)
                   GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302535.712883/94‑1 (MQ=60)
                   GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAG                                                                            >  SRR4302535.1308715/1‑94 (MQ=60)
                    TCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGA                                                                           >  SRR4302535.558752/1‑94 (MQ=60)
                    TCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGA                                                                           >  SRR4302535.1692139/1‑94 (MQ=60)
                     CCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAA                                                                          >  SRR4302535.4329287/1‑94 (MQ=60)
                      CATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAG                                                                         <  SRR4302535.738237/94‑1 (MQ=60)
                      CATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAG                                                                         <  SRR4302535.4498138/94‑1 (MQ=60)
                      CATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAG                                                                         <  SRR4302535.1464209/94‑1 (MQ=60)
                       ATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGA                                                                        <  SRR4302535.2232803/94‑1 (MQ=60)
                       ATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGA                                                                        <  SRR4302535.3808240/94‑1 (MQ=60)
                        TAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAA                                                                       >  SRR4302535.2143455/1‑94 (MQ=60)
                        TAACGTCCGGCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAA                                                                       <  SRR4302535.1063929/94‑1 (MQ=60)
                         AACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAG                                                                      <  SRR4302535.2374200/94‑1 (MQ=60)
                         AACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAG                                                                      <  SRR4302535.543002/94‑1 (MQ=60)
                         AACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAG                                                                      <  SRR4302535.2681322/94‑1 (MQ=60)
                         AACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAG                                                                      <  SRR4302535.299395/94‑1 (MQ=60)
                          ACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGT                                                                     <  SRR4302535.3170960/94‑1 (MQ=60)
                           CGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTT                                                                    <  SRR4302535.4643943/94‑1 (MQ=60)
                            GTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTT                                                                   <  SRR4302535.4319906/94‑1 (MQ=60)
                             TCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTT                                                                  <  SRR4302535.4535957/94‑1 (MQ=60)
                             TCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTT                                                                  <  SRR4302535.459550/94‑1 (MQ=60)
                              CCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTC                                                                 >  SRR4302535.3222849/1‑94 (MQ=60)
                               CGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCC                                                                <  SRR4302535.2640887/94‑1 (MQ=60)
                                GTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCC                                                               >  SRR4302535.1088926/1‑94 (MQ=60)
                                GTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTCAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCA                                                               <  SRR4302535.3411202/94‑1 (MQ=60)
                                  CGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACC                                                             >  SRR4302535.3467308/1‑94 (MQ=60)
                                   GGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCG                                                            >  SRR4302535.2791571/1‑94 (MQ=60)
                                   CGGTACAGATTGAACAACTGTCGCCCACTTTCTGTCTCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCG                                                            <  SRR4302535.3925729/94‑1 (MQ=60)
                                     GTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCT                                                          <  SRR4302535.609874/94‑1 (MQ=60)
                                     GTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCT                                                          >  SRR4302535.1309173/1‑94 (MQ=60)
                                     GTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCT                                                          <  SRR4302535.30502/94‑1 (MQ=60)
                                      TACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTG                                                         >  SRR4302535.792146/1‑94 (MQ=60)
                                       ACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGC                                                        >  SRR4302535.3632288/1‑94 (MQ=60)
                                        CAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCG                                                       <  SRR4302535.3707521/94‑1 (MQ=60)
                                        CAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCG                                                       >  SRR4302535.3314139/1‑94 (MQ=60)
                                        CAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCG                                                       <  SRR4302535.2833337/94‑1 (MQ=60)
                                         AGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGG                                                      >  SRR4302535.605734/1‑94 (MQ=60)
                                          GATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGT                                                     <  SRR4302535.2486638/94‑1 (MQ=60)
                                           ATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTC                                                    >  SRR4302535.2936239/1‑94 (MQ=60)
                                            TTGAACAACTGTCTCGCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCT                                                   <  SRR4302535.1478061/94‑1 (MQ=60)
                                            TTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCT                                                   <  SRR4302535.3642970/94‑1 (MQ=60)
                                            TTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCT                                                   <  SRR4302535.237219/94‑1 (MQ=60)
                                            TTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCT                                                   <  SRR4302535.1162444/94‑1 (MQ=60)
                                             TGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTG                                                  >  SRR4302535.1594839/1‑94 (MQ=60)
                                               AACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGA                                                >  SRR4302535.963637/1‑94 (MQ=60)
                                                 CAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGCCTGGAAG                                              >  SRR4302535.486081/1‑94 (MQ=60)
                                                  AACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGG                                             <  SRR4302535.503244/94‑1 (MQ=60)
                                                   ACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGA                                            >  SRR4302535.3018032/1‑94 (MQ=60)
                                                   ACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGA                                            >  SRR4302535.2835936/1‑94 (MQ=60)
                                                    CTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGCT                                           >  SRR4302535.1003472/1‑94 (MQ=60)
                                                    CTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGAT                                           >  SRR4302535.3404600/1‑94 (MQ=60)
                                                     TGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATT                                          >  SRR4302535.2509788/1‑94 (MQ=60)
                                                      GTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTA                                         <  SRR4302535.201812/94‑1 (MQ=60)
                                                      GTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTA                                         <  SRR4302535.2876986/94‑1 (MQ=60)
                                                      GTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTA                                         >  SRR4302535.1160755/1‑94 (MQ=60)
                                                        CGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAG                                       >  SRR4302535.3149439/1‑94 (MQ=60)
                                                        CGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAG                                       >  SRR4302535.500508/1‑94 (MQ=60)
                                                        CGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAG                                       <  SRR4302535.1766253/94‑1 (MQ=60)
                                                        CGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAG                                       <  SRR4302535.3140232/94‑1 (MQ=60)
                                                        CGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAG                                       <  SRR4302535.628094/94‑1 (MQ=60)
                                                        CGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAG                                       >  SRR4302535.3862941/1‑94 (MQ=60)
                                                        CGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAAAGCCAAACGTGGAAGAAGGTTTACACCGCTGCGGTCTGGAAGGATTAAG                                       >  SRR4302535.3862942/1‑94 (MQ=60)
                                                          CCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTT                                     <  SRR4302535.2071713/94‑1 (MQ=60)
                                                          CCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTT                                     <  SRR4302535.1746404/94‑1 (MQ=60)
                                                            CACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGC                                   >  SRR4302535.3745338/1‑94 (MQ=60)
                                                            CACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGC                                   >  SRR4302535.3019863/1‑94 (MQ=60)
                                                              CTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCG                                 >  SRR4302535.292160/1‑94 (MQ=60)
                                                                  CTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATG                             <  SRR4302535.3700581/94‑1 (MQ=60)
                                                                  CTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATG                             <  SRR4302535.265308/94‑1 (MQ=60)
                                                                  CTGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATG                             >  SRR4302535.3102675/1‑94 (MQ=60)
                                                                   TGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGA                            <  SRR4302535.2777353/94‑1 (MQ=60)
                                                                   TGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGA                            <  SRR4302535.4474361/94‑1 (MQ=60)
                                                                   TGGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGA                            <  SRR4302535.3142574/94‑1 (MQ=60)
                                                                    GGCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGAT                           <  SRR4302535.2637250/94‑1 (MQ=60)
                                                                     GCGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATG                          <  SRR4302535.512645/94‑1 (MQ=60)
                                                                      CGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGG                         <  SRR4302535.3571081/94‑1 (MQ=60)
                                                                      CGCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGG                         <  SRR4302535.3018908/94‑1 (MQ=60)
                                                                       GCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGA                        >  SRR4302535.1399659/1‑94 (MQ=60)
                                                                       GCGTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGA                        <  SRR4302535.4344027/94‑1 (MQ=60)
                                                                         GTGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAA                      >  SRR4302535.4269535/1‑94 (MQ=60)
                                                                          TGGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAC                     >  SRR4302535.3429223/1‑94 (MQ=60)
                                                                           GGTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACC                    <  SRR4302535.3033848/94‑1 (MQ=60)
                                                                            GTGGCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCT                   <  SRR4302535.4344543/94‑1 (MQ=60)
                                                                             TGGCAAGTGATCGGTG‑GCGAGAAAGGCGAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTC                  <  SRR4302535.1333522/94‑1 (MQ=60)
                                                                               GCAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCT                <  SRR4302535.284813/94‑1 (MQ=60)
                                                                                CAAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTT               <  SRR4302535.671491/94‑1 (MQ=60)
                                                                                 AAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTC              >  SRR4302535.543294/1‑94 (MQ=60)
                                                                                 AAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTC              <  SRR4302535.3450891/94‑1 (MQ=60)
                                                                                 AAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTC              <  SRR4302535.3056390/94‑1 (MQ=60)
                                                                                 AAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTC              <  SRR4302535.4163576/94‑1 (MQ=60)
                                                                                 AAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCAGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTC              <  SRR4302535.1771069/94‑1 (MQ=60)
                                                                                 AAGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTGTACACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTC              <  SRR4302535.2373294/94‑1 (MQ=60)
                                                                                  AGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             >  SRR4302535.4226982/1‑94 (MQ=60)
                                                                                  AGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             >  SRR4302535.2708742/1‑94 (MQ=60)
                                                                                  AGTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             >  SRR4302535.3196996/1‑94 (MQ=60)
                                                                                   GTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTT            <  SRR4302535.3192244/94‑1 (MQ=60)
                                                                                   GTTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTT            <  SRR4302535.19253/94‑1 (MQ=60)
                                                                                    TTAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTT           <  SRR4302535.1143062/94‑1 (MQ=60)
                                                                                     TAT‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTA          <  SRR4302535.195766/94‑1 (MQ=60)
                                                                                       T‑GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATG        >  SRR4302535.4449535/1‑94 (MQ=60)
                                                                                       T‑GGTG‑GCGAGAAACGCCAAACGTAGAAAGAGTTTTCCCCCCGTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGT       <  SRR4302535.2397810/94‑1 (MQ=60)
                                                                                          GTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTC      <  SRR4302535.2957642/94‑1 (MQ=60)
                                                                                          GTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTC      <  SRR4302535.2091429/94‑1 (MQ=60)
                                                                                           ttgGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCG     <  SRR4302535.949356/91‑1 (MQ=60)
                                                                                           ttgGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCG     <  SRR4302535.282069/91‑1 (MQ=60)
                                                                                              GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  <  SRR4302535.4537013/94‑1 (MQ=60)
                                                                                              GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  <  SRR4302535.2121323/94‑1 (MQ=60)
                                                                                              |                                                                                             
CACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT‑GGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  >  NC_000913/3930476‑3930661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑