Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,938,723	C→T	R28R (CGG→CGA)	yiePs ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,938,723	0	C	T	99.2%	890.7 / ‑4.6	245	R28R (CGG→CGA)	yiePs	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (143/100); total (145/100)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 249 total aligned reads displayed.

GTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAA  >  NC_000913/3938633‑3938812

 tGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGa                                                                                        <  2:1991653/93‑1 (MQ=255)

  gATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAg                                                                                       <  2:2016502/93‑1 (MQ=255)

      gATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAATTTCCAGTTCGATTGTGGCATGACTCGAGTAcc                                                                                   >  1:137271/1‑93 (MQ=255)

      gATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTAcc                                                                                   >  1:3181744/1‑93 (MQ=255)

      gATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTAcc                                                                                   >  1:2090958/1‑93 (MQ=255)

       aTGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCa                                                                                  >  2:4586878/1‑93 (MQ=255)

        tGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCaa                                                                                 >  1:1952877/1‑93 (MQ=255)

         gACCTGATGAAAGTTCTCTTCTGTCATCAACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAAt                                                                                <  1:4509010/93‑1 (MQ=255)

           ccTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtc                                                                              <  2:2961256/93‑1 (MQ=255)

           ccTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtc                                                                              >  2:1908878/1‑93 (MQ=255)

             tgatgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgc                                                                            >  2:3183356/1‑93 (MQ=255)

             tgatgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgc                                                                            >  1:4658257/1‑93 (MQ=255)

             tgatgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgc                                                                            >  1:4591533/1‑93 (MQ=255)

              gatgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcg                                                                           >  1:2716957/1‑93 (MQ=255)

              gatgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACAAATtcgcg                                                                           >  1:3588068/1‑93 (MQ=255)

                tgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcggt                                                                         <  2:4110728/93‑1 (MQ=255)

                tgaAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcggt                                                                         <  1:739322/93‑1 (MQ=255)

                   aaGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcggtcgc                                                                      <  1:1928741/93‑1 (MQ=255)

                    aGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcggtcgcg                                                                     >  2:4382434/1‑93 (MQ=255)

                     gTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATtcgcggtcgcgg                                                                    <  2:2967839/93‑1 (MQ=255)

                      ttCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTcgcggtcgcggt                                                                   >  1:577653/1‑93 (MQ=255)

                       tctcTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGATTCTAGTACCAATTCGCGGTCGCGGTa                                                                  >  2:725202/1‑93 (MQ=255)

                       tctcTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTa                                                                  >  1:669302/1‑93 (MQ=255)

                        ctctTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTaa                                                                 <  1:2929120/93‑1 (MQ=255)

                        ctctTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTaa                                                                 <  1:927981/93‑1 (MQ=255)

                         tcttctGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTaaa                                                                >  2:2511359/1‑93 (MQ=255)

                           ttctGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAc                                                              <  1:796346/93‑1 (MQ=255)

                            tctGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAcc                                                             >  1:1702028/1‑93 (MQ=255)

                              tGTCATCCACCAGGTAAGCAATTCCTAATAAAGAAAATTCCAGTTCGATTGTGGCATGCCTCGAGTACCAATTCGCGGTCGCGGTAAAACCAt                                                           <  2:1878620/93‑1 (MQ=255)

                               gTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATc                                                          <  1:4196217/93‑1 (MQ=255)

                               gTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATc                                                          >  1:3999677/1‑93 (MQ=255)

                                 cATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATccc                                                        <  1:1271701/93‑1 (MQ=255)

                                     caccaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCtttt                                                    <  1:4580030/93‑1 (MQ=255)

                                     caccaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCtttt                                                    <  1:3666140/93‑1 (MQ=255)

                                     caccaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCtttt                                                    <  1:4407042/93‑1 (MQ=255)

                                     caccaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCtttt                                                    <  1:3145999/93‑1 (MQ=255)

                                      accaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTg                                                   <  2:3807271/93‑1 (MQ=255)

                                       ccaGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGc                                                  >  1:3704746/1‑93 (MQ=255)

                                        caGGTAAGCAATTCCTGATCAAGAAAATTCCCGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGcg                                                 >  1:4460395/1‑92 (MQ=255)

                                        caGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGcc                                                 >  1:4112192/1‑93 (MQ=255)

                                        caGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGcc                                                 >  1:1252117/1‑93 (MQ=255)

                                        caGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGcc                                                 >  2:183909/1‑93 (MQ=255)

                                         aGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCg                                                >  2:4480113/1‑93 (MQ=255)

                                          ggTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGt                                               >  2:1515247/1‑93 (MQ=255)

                                          ggTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGt                                               >  1:2760092/1‑93 (MQ=255)

                                          ggTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGt                                               >  2:1412099/1‑93 (MQ=255)

                                           gTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGtt                                              <  2:2880195/93‑1 (MQ=255)

                                            tAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTa                                             <  1:3413327/93‑1 (MQ=255)

                                             aaGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTaa                                            <  2:422932/93‑1 (MQ=255)

                                               gCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACg                                          <  1:1665632/93‑1 (MQ=255)

                                               gCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACg                                          >  1:1117130/1‑93 (MQ=255)

                                               gCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACg                                          >  1:2181281/1‑93 (MQ=255)

                                               gCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGAGGTAAAACCATCCCTTTTGCCGTTAACg                                          >  1:4233407/1‑93 (MQ=255)

                                                cAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGt                                         >  2:2437011/1‑93 (MQ=255)

                                                  aTTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGttt                                       >  1:1737919/1‑93 (MQ=255)

                                                  aTTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGttt                                       <  2:3049649/93‑1 (MQ=255)

                                                   ttCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGtttt                                      >  2:474156/1‑93 (MQ=255)

                                                   ttCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGtttt                                      >  1:4552161/1‑93 (MQ=255)

                                                   ttCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGtttt                                      <  1:949890/93‑1 (MQ=255)

                                                    tCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTg                                     <  1:284666/93‑1 (MQ=255)

                                                    tCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTg                                     <  2:2309104/93‑1 (MQ=255)

                                                     ccTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGa                                    <  1:4328109/93‑1 (MQ=255)

                                                     ccTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGa                                    >  1:464381/1‑93 (MQ=255)

                                                      cTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAc                                   >  1:1351024/1‑93 (MQ=255)

                                                      cTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTCAc                                   >  1:3909566/1‑93 (MQ=255)

                                                       tGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGaac                                  >  2:1226681/1‑91 (MQ=255)

                                                       tGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAcc                                  <  1:1002030/93‑1 (MQ=255)

                                                       tGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAcc                                  >  1:1145197/1‑93 (MQ=255)

                                                        gATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCg                                 >  2:3261878/1‑93 (MQ=255)

                                                        gATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCg                                 >  1:3361507/1‑93 (MQ=255)

                                                          tCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCt                               >  2:3095445/1‑93 (MQ=255)

                                                           cAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCtt                              <  2:3903858/93‑1 (MQ=255)

                                                            aaGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTc                             <  2:568953/93‑1 (MQ=255)

                                                            aaGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTc                             <  1:322789/93‑1 (MQ=255)

                                                            aaGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTc                             <  1:507692/93‑1 (MQ=255)

                                                             aGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTcg                            <  2:2365727/93‑1 (MQ=255)

                                                             aGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTcg                            <  2:129218/93‑1 (MQ=255)

                                                             aGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTcg                            >  2:3868253/1‑93 (MQ=255)

                                                               aaaaTTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTcgcg                          <  1:3727889/93‑1 (MQ=255)

                                                                aaaTTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGt                         >  2:4664274/1‑93 (MQ=255)

                                                                aaaTTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGt                         >  2:2572463/1‑93 (MQ=255)

                                                                 aaTTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTa                        >  2:1108503/1‑93 (MQ=255)

                                                                  aTTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAc                       >  2:2118426/1‑93 (MQ=255)

                                                                      cAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCt                   <  1:3798186/93‑1 (MQ=255)

                                                                        gTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGt                 <  1:3118270/93‑1 (MQ=255)

                                                                         ttCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTa                <  1:1522196/93‑1 (MQ=255)

                                                                            gATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACgc             >  2:340367/1‑92 (MQ=255)

                                                                            gATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGa             >  2:3556070/1‑93 (MQ=255)

                                                                            gATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGa             >  2:4501032/1‑93 (MQ=255)

                                                                            gATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGa             >  1:2226806/1‑93 (MQ=255)

                                                                             aTTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGac            <  2:2245420/93‑1 (MQ=255)

                                                                             aTTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGac            >  1:2081789/1‑93 (MQ=255)

                                                                             aTTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGac            >  1:3827916/1‑93 (MQ=255)

                                                                              ttGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGact           >  2:2662860/1‑93 (MQ=255)

                                                                                gtgGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGactca         <  2:692268/93‑1 (MQ=255)

                                                                                  ggCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGactcact       <  1:1593312/93‑1 (MQ=255)

                                                                                  ggCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGactcact       >  2:1677702/1‑93 (MQ=255)

                                                                                     aTGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCa    >  2:1534172/1‑93 (MQ=255)

                                                                                      tGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCaa   >  2:1772201/1‑93 (MQ=255)

                                                                                       gACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCaaa  <  1:1551745/93‑1 (MQ=255)

GTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAA  >  NC_000913/3938633‑3938812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAAT  >  NC_000913/3938630‑3938813

AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACT                                                                                            <  SRR4302535.4400274/94‑1 (MQ=60)

AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACT                                                                                            >  SRR4302535.957124/1‑94 (MQ=60)

AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACT                                                                                            >  SRR4302535.657485/1‑94 (MQ=60)

AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACT                                                                                            <  SRR4302535.1772514/94‑1 (MQ=60)

AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACT                                                                                            <  SRR4302535.252914/94‑1 (MQ=60)

 AAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCACGACTC                                                                                           >  SRR4302535.4557922/1‑94 (MQ=60)

  AGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCG                                                                                          >  SRR4302535.1327537/1‑94 (MQ=60)

     GATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGT                                                                                       <  SRR4302535.2734766/94‑1 (MQ=60)

      ATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTA                                                                                      >  SRR4302535.3747811/1‑94 (MQ=60)

      ATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTA                                                                                      >  SRR4302535.4689069/1‑94 (MQ=60)

      ATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTA                                                                                      >  SRR4302535.4542846/1‑94 (MQ=60)

        CGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAATTTCCAGTTCGATTGTGGCATGACTCGAGTACC                                                                                    >  SRR4302535.138234/1‑94 (MQ=60)

        CGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACC                                                                                    >  SRR4302535.2106025/1‑94 (MQ=60)

        CGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACC                                                                                    >  SRR4302535.2106026/1‑94 (MQ=60)

        CGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACC                                                                                    >  SRR4302535.93530/1‑94 (MQ=60)

        CGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACC                                                                                    >  SRR4302535.3203734/1‑94 (MQ=60)

        CGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACC                                                                                    >  SRR4302535.3694062/1‑94 (MQ=60)

        CGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACC                                                                                    >  SRR4302535.4650947/1‑94 (MQ=60)

          ATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAA                                                                                  >  SRR4302535.2785301/1‑94 (MQ=60)

          ATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAA                                                                                  >  SRR4302535.1966934/1‑94 (MQ=60)

           TGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAAT                                                                                 >  SRR4302535.2220876/1‑94 (MQ=60)

           TGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAAT                                                                                 >  SRR4302535.314415/1‑94 (MQ=60)

            GACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATT                                                                                <  SRR4302535.2914115/94‑1 (MQ=60)

            GACCTGATGAAAGTTCTCTTCTGTCATCAACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATT                                                                                <  SRR4302535.4539950/94‑1 (MQ=60)

             ACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTC                                                                               <  SRR4302535.1957779/94‑1 (MQ=60)

             ACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTC                                                                               <  SRR4302535.2769935/94‑1 (MQ=60)

               CTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGC                                                                             >  SRR4302535.4689770/1‑94 (MQ=60)

               CTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGC                                                                             >  SRR4302535.4622788/1‑94 (MQ=60)

               CTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGC                                                                             >  SRR4302535.4622751/1‑94 (MQ=60)

                TGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCG                                                                            >  SRR4302535.2736366/1‑94 (MQ=60)

                TGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACAAATTCGCG                                                                            >  SRR4302535.3612957/1‑94 (MQ=60)

                   TGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTC                                                                         <  SRR4302535.744519/94‑1 (MQ=60)

                   TGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTC                                                                         <  SRR4302535.1194192/94‑1 (MQ=60)

                    TAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCG                                                                        <  SRR4302535.813361/94‑1 (MQ=60)

                    GAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCG                                                                        >  SRR4302535.304327/1‑94 (MQ=60)

                     AAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGC                                                                       >  SRR4302535.4056520/1‑94 (MQ=60)

                      AAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCG                                                                      <  SRR4302535.1942641/94‑1 (MQ=60)

                      AAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCG                                                                      >  SRR4302535.4424005/1‑94 (MQ=60)

                      AAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCG                                                                      <  SRR4302535.2852924/94‑1 (MQ=60)

                        GTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGT                                                                    >  SRR4302535.1234102/1‑94 (MQ=60)

                        GTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGT                                                                    >  SRR4302535.581709/1‑94 (MQ=60)

                         TTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTA                                                                   >  SRR4302535.2004653/1‑94 (MQ=60)

                         TTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTA                                                                   >  SRR4302535.673974/1‑94 (MQ=60)

                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                 <  SRR4302535.2949759/94‑1 (MQ=60)

                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                 <  SRR4302535.918096/94‑1 (MQ=60)

                           CTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAA                                                                 <  SRR4302535.934553/94‑1 (MQ=60)

                             CTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAC                                                               >  SRR4302535.1413022/1‑94 (MQ=60)

                             CTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAC                                                               >  SRR4302535.3912056/1‑94 (MQ=60)

                              TTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACC                                                              <  SRR4302535.801936/94‑1 (MQ=60)

                              TTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACC                                                              >  SRR4302535.4250875/1‑94 (MQ=60)

                              TTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACC                                                              >  SRR4302535.1714332/1‑94 (MQ=60)

                                CTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCAT                                                            <  SRR4302535.4242376/94‑1 (MQ=60)

                                CTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCAT                                                            >  SRR4302535.1413002/1‑94 (MQ=60)

                                 TGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATC                                                           >  SRR4302535.216945/1‑94 (MQ=60)

                                 TGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATC                                                           <  SRR4302535.3249824/94‑1 (MQ=60)

                                 TGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATC                                                           >  SRR4302535.4027040/1‑94 (MQ=60)

                                  GTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCC                                                          <  SRR4302535.4224933/94‑1 (MQ=60)

                                  GTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCC                                                          <  SRR4302535.3624480/94‑1 (MQ=60)

                                    CATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCT                                                        <  SRR4302535.1280788/94‑1 (MQ=60)

                                     ATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTT                                                       <  SRR4302535.1093800/94‑1 (MQ=60)

                                     ATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAAACATCCCTT                                                       <  SRR4302535.2168458/94‑1 (MQ=60)

                                      TCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTT                                                      >  SRR4302535.3608382/1‑94 (MQ=60)

                                       CCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTT                                                     <  SRR4302535.2306805/94‑1 (MQ=60)

                                       CCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTT                                                     <  SRR4302535.1695116/94‑1 (MQ=60)

                                        CACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTG                                                    <  SRR4302535.3691527/94‑1 (MQ=60)

                                        CACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTG                                                    <  SRR4302535.4611225/94‑1 (MQ=60)

                                        CACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTG                                                    <  SRR4302535.1818390/94‑1 (MQ=60)

                                        CACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTG                                                    <  SRR4302535.4437202/94‑1 (MQ=60)

                                        CACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTG                                                    <  SRR4302535.3167877/94‑1 (MQ=60)

                                         ACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGC                                                   >  SRR4302535.1606718/1‑94 (MQ=60)

                                         ACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGC                                                   >  SRR4302535.3730405/1‑94 (MQ=60)

                                          CCAGGTAAGCAATTCCTGATCAAGAAAATTCCCGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCG                                                  >  SRR4302535.4490988/1‑94 (MQ=60)

                                          CCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCC                                                  >  SRR4302535.4140338/1‑94 (MQ=60)

                                          CCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCC                                                  >  SRR4302535.697237/1‑94 (MQ=60)

                                          CCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCC                                                  >  SRR4302535.1261057/1‑94 (MQ=60)

                                          CCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTAGCGGTCGCGGTAAAACCATCCCTTTTGCC                                                  >  SRR4302535.3407076/1‑94 (MQ=60)

                                           CAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCG                                                 <  SRR4302535.986175/94‑1 (MQ=60)

                                           CAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCG                                                 <  SRR4302535.1739292/94‑1 (MQ=60)

                                            AGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGT                                                >  SRR4302535.2779761/1‑94 (MQ=60)

                                             GGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTT                                               <  SRR4302535.3366359/94‑1 (MQ=60)

                                               TAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAA                                             <  SRR4302535.3437076/94‑1 (MQ=60)

                                               TAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAA                                             <  SRR4302535.1344721/94‑1 (MQ=60)

                                                 AGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACG                                           >  SRR4302535.2196842/1‑94 (MQ=60)

                                                 AGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACG                                           >  SRR4302535.1125096/1‑94 (MQ=60)

                                                 AGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGAGGTAAAACCATCCCTTTTGCCGTTAACG                                           >  SRR4302535.4262377/1‑94 (MQ=60)

                                                  GCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGT                                          <  SRR4302535.1677684/94‑1 (MQ=60)

                                                    AATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTT                                        >  SRR4302535.3008460/1‑94 (MQ=60)

                                                    AATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTT                                        >  SRR4302535.1750479/1‑94 (MQ=60)

                                                     ATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTT                                       >  SRR4302535.3878202/1‑94 (MQ=60)

                                                     ATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTT                                       >  SRR4302535.12673/1‑94 (MQ=60)

                                                     ATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTT                                       >  SRR4302535.4583269/1‑94 (MQ=60)

                                                     ATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTT                                       <  SRR4302535.649970/94‑1 (MQ=60)

                                                      TTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTG                                      <  SRR4302535.956616/94‑1 (MQ=60)

                                                       TCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGA                                     <  SRR4302535.286655/94‑1 (MQ=60)

                                                       TCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGA                                     >  SRR4302535.3775572/1‑94 (MQ=60)

                                                       TCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGA                                     >  SRR4302535.467626/1‑94 (MQ=60)

                                                        CCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAC                                    <  SRR4302535.4357704/94‑1 (MQ=60)

                                                        CCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAC                                    <  SRR4302535.1785919/94‑1 (MQ=60)

                                                        CCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAC                                    <  SRR4302535.4521250/94‑1 (MQ=60)

                                                        CCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGAC                                    >  SRR4302535.1360697/1‑94 (MQ=60)

                                                        CCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTCAC                                    >  SRR4302535.3936340/1‑94 (MQ=60)

                                                         CTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACC                                   >  SRR4302535.3199900/1‑94 (MQ=60)

                                                         CTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACC                                   <  SRR4302535.3291123/94‑1 (MQ=60)

                                                         CTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACC                                   >  SRR4302535.1153358/1‑94 (MQ=60)

                                                          TGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCG                                  >  SRR4302535.1365766/1‑94 (MQ=60)

                                                          TGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCG                                  >  SRR4302535.3384900/1‑94 (MQ=60)

                                                          TGATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCG                                  <  SRR4302535.1009148/94‑1 (MQ=60)

                                                           GATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGC                                 >  SRR4302535.1568016/1‑94 (MQ=60)

                                                           GATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGC                                 >  SRR4302535.4602310/1‑94 (MQ=60)

                                                            ATCAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCT                                <  SRR4302535.369600/94‑1 (MQ=60)

                                                            ATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCT                                >  SRR4302535.3758707/1‑94 (MQ=60)

                                                              CAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTC                              >  SRR4302535.450515/1‑94 (MQ=60)

                                                              CAAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTC                              <  SRR4302535.4466223/94‑1 (MQ=60)

                                                               AAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCG                             <  SRR4302535.511241/94‑1 (MQ=60)

                                                               AAGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCG                             <  SRR4302535.325021/94‑1 (MQ=60)

                                                                AGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACTGCTTCGC                            >  SRR4302535.4660803/1‑94 (MQ=60)

                                                                AGAAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGC                            >  SRR4302535.48322/1‑94 (MQ=60)

                                                                  AAAATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGT                          <  SRR4302535.3753711/94‑1 (MQ=60)

                                                                    AATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAC                        <  SRR4302535.769565/94‑1 (MQ=60)

                                                                    AATTCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTAC                        <  SRR4302535.1564281/94‑1 (MQ=60)

                                                                       TCCAGTTCGATTTTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGC                     >  SRR4302535.1562346/1‑94 (MQ=60)

                                                                       TCCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGC                     >  SRR4302535.968359/1‑94 (MQ=60)

                                                                        CCAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCT                    >  SRR4302535.3177693/1‑94 (MQ=60)

                                                                         CAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTG                   <  SRR4302535.3824371/94‑1 (MQ=60)

                                                                         CAGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTG                   >  SRR4302535.4407289/1‑94 (MQ=60)

                                                                          AGTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGT                  >  SRR4302535.3522162/1‑94 (MQ=60)

                                                                          AGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGT                  >  SRR4302535.402656/1‑94 (MQ=60)

                                                                           GTTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTA                 <  SRR4302535.3139980/94‑1 (MQ=60)

                                                                            TTCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTAC                <  SRR4302535.1533159/94‑1 (MQ=60)

                                                                             TCGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACG               <  SRR4302535.4591507/94‑1 (MQ=60)

                                                                              CGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGA              >  SRR4302535.413199/1‑94 (MQ=60)

                                                                              CGATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGA              >  SRR4302535.2242649/1‑94 (MQ=60)

                                                                              CGATTGTGGCATGACTCGAGTACCAATTCGCGGCCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGA              >  SRR4302535.505327/1‑94 (MQ=60)

                                                                               GATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGAC             >  SRR4302535.1143363/1‑94 (MQ=60)

                                                                               GATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGAC             >  SRR4302535.3854319/1‑94 (MQ=60)

                                                                               GATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGAC             >  SRR4302535.2096788/1‑94 (MQ=60)

                                                                                ATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACT            <  SRR4302535.2073142/94‑1 (MQ=60)

                                                                                ATTGTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACT            >  SRR4302535.4218998/1‑94 (MQ=60)

                                                                                   GTGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCAC         <  SRR4302535.1698475/94‑1 (MQ=60)

                                                                                    TGGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACTGCTGTACGACTCACT        >  SRR4302535.709792/1‑94 (MQ=60)

                                                                                     GGCATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTC       <  SRR4302535.1604810/94‑1 (MQ=60)

                                                                                       CATGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCA     >  SRR4302535.516713/1‑94 (MQ=60)

                                                                                         TGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAA   >  SRR4302535.3835352/1‑94 (MQ=60)

                                                                                         TGACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAA   >  SRR4302535.4329964/1‑94 (MQ=60)

                                                                                          GACTCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAAT  <  SRR4302535.1562923/94‑1 (MQ=60)

AAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAGCAATTCCTGATCAAGAAAATTCCAGTTCGATTGTGGCATGACCCGAGTACCAATTCGCGGTCGCGGTAAAACCATCCCTTTTGCCGTTAACGTTTTGACCGCTTCGCGTACCGCTGTACGACTCACTCCAAAT  >  NC_000913/3938630‑3938813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`