Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,958,877	2 bp→CG	coding (586‑587/2022 nt)	rep →	rep helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,958,877	0	G	C	100.0%	811.2 / NA	252	A196R (GCC→CGC)	rep	rep helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (119/133); total (119/133)
*	NC_000913	3,958,878	0	C	G	99.6%	813.2 / ‑5.6	252	A196R (GCC→CGC)	rep	rep helicase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (119/132); minor base T (0/1); total (119/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 259 total aligned reads displayed.

TTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG  >  NC_000913/3958792‑3958964

||

ttATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATga                                                                                  >  2:2657591/1‑93 (MQ=255)

  aTGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaag                                                                                <  1:3036914/93‑1 (MQ=255)

    gggCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATgaagaa                                                                              <  2:2148636/93‑1 (MQ=255)

      gCTGTATGATGCTCACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGt                                                                            <  1:159457/93‑1 (MQ=255)

      gCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGt                                                                            <  2:3448739/93‑1 (MQ=255)

       cTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTc                                                                           <  1:4285342/93‑1 (MQ=255)

        tGTATGATGCACACCTGAATGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGACGAAGTcc                                                                          >  2:4572455/1‑93 (MQ=255)

        tGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTcc                                                                          >  2:888294/1‑93 (MQ=255)

        tGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTcc                                                                          <  2:4668726/93‑1 (MQ=255)

           atgatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCa                                                                       >  2:819873/1‑93 (MQ=255)

            tgatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCaa                                                                      >  1:4646006/1‑93 (MQ=255)

             gatgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAg                                                                     >  1:122726/1‑93 (MQ=255)

              atgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgc                                                                    >  2:3131640/1‑93 (MQ=255)

              atgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgc                                                                    <  1:3002786/93‑1 (MQ=255)

               tgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcg                                                                   <  1:3629678/93‑1 (MQ=255)

               tgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcg                                                                   >  1:1079234/1‑93 (MQ=255)

               tgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcg                                                                   >  1:2577354/1‑93 (MQ=255)

               tgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcg                                                                   >  2:1524926/1‑93 (MQ=255)

               tgCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcg                                                                   >  2:4301862/1‑93 (MQ=255)

                gCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAgcgc                                                                  <  1:548751/93‑1 (MQ=255)

                   cacCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTgg                                                               >  2:3004082/1‑93 (MQ=255)

                     ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             >  1:2433621/1‑93 (MQ=255)

                     ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             <  2:3235671/93‑1 (MQ=255)

                     ccTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCa                                                             <  1:2929242/93‑1 (MQ=255)

                        gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          >  1:2369014/1‑93 (MQ=255)

                        gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          <  2:3057864/93‑1 (MQ=255)

                        gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          <  2:3616208/93‑1 (MQ=255)

                        gAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGaa                                                          >  2:3987577/1‑93 (MQ=255)

                         aaaGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAAc                                                         >  1:2211260/1‑93 (MQ=255)

                         aaaGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAAc                                                         <  1:2650272/93‑1 (MQ=255)

                           aGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGCTGCTTCTGCAACGCAATGAAGAAGTCAGCAAGCGCTCGCAGaaaca                                                       >  1:651766/1‑90 (MQ=255)

                             ccTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                                                     >  1:1753187/1‑93 (MQ=255)

                             ccTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                                                     <  2:2786736/93‑1 (MQ=255)

                             ccTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                                                     <  2:1563676/93‑1 (MQ=255)

                             ccTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                                                     >  2:1655397/1‑93 (MQ=255)

                             ccTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACaaaa                                                     <  1:1292075/93‑1 (MQ=255)

                               tGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAtt                                                   >  2:2629674/1‑93 (MQ=255)

                               tGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAtt                                                   <  2:4246256/93‑1 (MQ=255)

                                gTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTc                                                  >  2:3572756/1‑93 (MQ=255)

                                gTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTc                                                  >  2:4107177/1‑93 (MQ=255)

                                 tAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCg                                                 >  1:725763/1‑93 (MQ=255)

                                  aaCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGc                                                <  2:3853742/93‑1 (MQ=255)

                                  aaCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGc                                                <  1:3473714/93‑1 (MQ=255)

                                  aaCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTACGCAAGCGCTGGCACAACAAAAATCGc                                                >  1:1340677/1‑93 (MQ=255)

                                   aCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCt                                               <  1:4496315/93‑1 (MQ=255)

                                   aCGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCt                                               <  2:4206410/93‑1 (MQ=255)

                                    cGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTa                                              <  2:2482574/93‑1 (MQ=255)

                                    cGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTa                                              <  2:4516802/93‑1 (MQ=255)

                                     gTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAt                                             <  1:1545813/93‑1 (MQ=255)

                                     gTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAt                                             >  1:1452248/1‑93 (MQ=255)

                                      ttCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATc                                            <  1:4128632/93‑1 (MQ=255)

                                      ttCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATc                                            >  1:3084044/1‑93 (MQ=255)

                                      ttCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATc                                            >  2:271159/1‑93 (MQ=255)

                                      ttCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATc                                            <  2:388374/93‑1 (MQ=255)

                                       tctcGACTTTGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATct                                           >  2:2501733/1‑93 (MQ=255)

                                       tctcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATct                                           <  1:845507/93‑1 (MQ=255)

                                         tcGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgc                                         <  2:1882052/93‑1 (MQ=255)

                                          cGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgct                                        <  1:1173201/93‑1 (MQ=255)

                                          cGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATctgct                                        <  1:3614665/93‑1 (MQ=255)

                                             cTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggt                                     <  2:584154/93‑1 (MQ=255)

                                              ttCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggtg                                    >  1:1325678/1‑93 (MQ=255)

                                              ttCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggtg                                    >  1:2011823/1‑93 (MQ=255)

                                               tCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggtgg                                   <  1:287831/93‑1 (MQ=255)

                                               tCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCtggtgg                                   >  2:1076297/1‑93 (MQ=255)

                                                 gatgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGAt                                 <  2:1721725/93‑1 (MQ=255)

                                                  atgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATg                                >  2:4183306/1‑93 (MQ=255)

                                                   tgatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGa                               <  1:2065823/93‑1 (MQ=255)

                                                    tatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              <  2:3383162/92‑1 (MQ=255)

                                                    gatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              >  1:3282913/1‑93 (MQ=255)

                                                    gatCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAg                              <  2:2105202/93‑1 (MQ=255)

                                                      tCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTGGCTATCTGCTGGTGGATGAGTa                            <  1:3881282/93‑1 (MQ=255)

                                                      tCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTa                            >  2:4450357/1‑93 (MQ=255)

                                                       cTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAt                           >  1:274779/1‑93 (MQ=255)

                                                         gATTTTATTGCCGACGTTGCTGCTGCAACTCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         <  2:4604000/93‑1 (MQ=255)

                                                         gATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCa                         <  1:961270/93‑1 (MQ=255)

                                                           ttttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAgg                       <  1:3347518/93‑1 (MQ=255)

                                                            tttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGCTGAGTATCAggc                      >  2:1971836/1‑92 (MQ=255)

                                                            tttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGa                      <  1:2988720/93‑1 (MQ=255)

                                                             ttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGac                     <  2:2848251/93‑1 (MQ=255)

                                                             ttATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAATCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGac                     <  1:1518953/93‑1 (MQ=255)

                                                               aTTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacac                   >  1:1932959/1‑93 (MQ=255)

                                                               aTTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacac                   <  2:437566/93‑1 (MQ=255)

                                                                   ccGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaac               <  1:354342/93‑1 (MQ=255)

                                                                    ctaCGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaaca              <  2:2994253/91‑1 (MQ=255)

                                                                    cGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaaca              <  1:2256218/93‑1 (MQ=255)

                                                                     gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             >  2:1019814/1‑93 (MQ=255)

                                                                     gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             >  1:774401/1‑93 (MQ=255)

                                                                     gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             >  2:219101/1‑93 (MQ=255)

                                                                     gACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTAGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacac             <  2:134260/93‑1 (MQ=255)

                                                                      aCGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacacc            >  2:1486975/1‑93 (MQ=255)

                                                                       cGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacacca           <  1:3490971/93‑1 (MQ=255)

                                                                       cGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGacaccaacacca           <  2:3309254/93‑1 (MQ=255)

                                                                        gTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccag          >  1:163726/1‑93 (MQ=255)

                                                                         ttGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAccagc         <  2:3778373/93‑1 (MQ=255)

                                                                             tgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAACTTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGt     <  1:4424211/93‑1 (MQ=255)

                                                                             tgctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGt     >  2:3241276/1‑93 (MQ=255)

                                                                              gctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTa    >  2:1259596/1‑93 (MQ=255)

                                                                              gctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTa    >  1:2295225/1‑93 (MQ=255)

                                                                              gctgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTa    >  1:3853732/1‑93 (MQ=255)

                                                                                tgcAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATg  <  2:307300/93‑1 (MQ=255)

||

TTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG  >  NC_000913/3958792‑3958964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  >  NC_000913/3958784‑3958966

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAC                                                                                           <  SRR4302535.1769333/94‑1 (MQ=60)

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAC                                                                                           <  SRR4302535.2560495/94‑1 (MQ=60)

       GTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGA                                                                                    >  SRR4302535.298102/1‑94 (MQ=60)

       GTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGA                                                                                    >  SRR4302535.2926911/1‑94 (MQ=60)

        TTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAA                                                                                   <  SRR4302535.149032/94‑1 (MQ=60)

        TTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAA                                                                                   >  SRR4302535.3405736/1‑94 (MQ=60)

        TTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAA                                                                                   <  SRR4302535.3052747/94‑1 (MQ=60)

         TATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAG                                                                                  >  SRR4302535.2608316/1‑94 (MQ=60)

          ATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGA                                                                                 <  SRR4302535.3058183/94‑1 (MQ=60)

          ATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGA                                                                                 <  SRR4302535.3021114/94‑1 (MQ=60)

           TGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAA                                                                                <  SRR4302535.906932/94‑1 (MQ=60)

           TGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAA                                                                                <  SRR4302535.4575971/94‑1 (MQ=60)

             GGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGT                                                                              >  SRR4302535.1153927/1‑94 (MQ=60)

              GCTGTATGATGCTCACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTC                                                                             <  SRR4302535.160575/94‑1 (MQ=60)

              GCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTC                                                                             <  SRR4302535.2702187/94‑1 (MQ=60)

              GCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTC                                                                             >  SRR4302535.2246308/1‑94 (MQ=60)

              GCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTAGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTCCAATGCAATGAAGAAGTC                                                                             >  SRR4302535.3333452/1‑94 (MQ=60)

               CTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCC                                                                            >  SRR4302535.2320793/1‑94 (MQ=60)

               CTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCC                                                                            <  SRR4302535.4314657/94‑1 (MQ=60)

               CTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCC                                                                            <  SRR4302535.1725318/94‑1 (MQ=60)

                TGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGCTGCTGCTGCAACGCAATGAAGAAGCCCG                                                                           >  SRR4302535.987506/1‑94 (MQ=60)

                 GTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGC                                                                          >  SRR4302535.756847/1‑94 (MQ=60)

                   ATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAA                                                                        >  SRR4302535.4677486/1‑94 (MQ=60)

                    TGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAG                                                                       >  SRR4302535.123588/1‑94 (MQ=60)

                    TGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAG                                                                       <  SRR4302535.2227568/94‑1 (MQ=60)

                      ATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCG                                                                     >  SRR4302535.1086914/1‑94 (MQ=60)

                      ATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCG                                                                     <  SRR4302535.3023833/94‑1 (MQ=60)

                      ATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCG                                                                     >  SRR4302535.2595875/1‑94 (MQ=60)

                       TGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGC                                                                    <  SRR4302535.3654836/94‑1 (MQ=60)

                        GCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCT                                                                   <  SRR4302535.552584/94‑1 (MQ=60)

                            ACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCA                                                               >  SRR4302535.2451153/1‑94 (MQ=60)

                             CCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAG                                                              <  SRR4302535.2949881/94‑1 (MQ=60)

                             CCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAG                                                              <  SRR4302535.753067/94‑1 (MQ=60)

                             CCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAG                                                              <  SRR4302535.3504276/94‑1 (MQ=60)

                              CTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGA                                                             <  SRR4302535.3783960/94‑1 (MQ=60)

                               TGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAA                                                            >  SRR4302535.2386022/1‑94 (MQ=60)

                               TGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAA                                                            <  SRR4302535.1529940/94‑1 (MQ=60)

                                GAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAAC                                                           >  SRR4302535.2227012/1‑94 (MQ=60)

                                GAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAAC                                                           <  SRR4302535.3495748/94‑1 (MQ=60)

                                 AAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACA                                                          <  SRR4302535.4689106/94‑1 (MQ=60)

                                 AAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACA                                                          <  SRR4302535.2669247/94‑1 (MQ=60)

                                 AAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACA                                                          <  SRR4302535.1723806/94‑1 (MQ=60)

                                  AAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGCTGCTTCTGCAACGCAATGAAGAAGTCAGCAAGCGCTCGCAGAAaca                                                         >  SRR4302535.656313/1‑91 (MQ=60)

                                    GCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAA                                                       >  SRR4302535.1765857/1‑94 (MQ=60)

                                    GCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAA                                                       <  SRR4302535.1232842/94‑1 (MQ=60)

                                    ACCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAA                                                       <  SRR4302535.1337647/94‑1 (MQ=60)

                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                      <  SRR4302535.1301282/94‑1 (MQ=60)

                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                      >  SRR4302535.1319650/1‑94 (MQ=60)

                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                      <  SRR4302535.163103/94‑1 (MQ=60)

                                     CCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAAT                                                      <  SRR4302535.3106279/94‑1 (MQ=60)

                                        GTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCG                                                   <  SRR4302535.401711/94‑1 (MQ=60)

                                        GTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCG                                                   >  SRR4302535.730858/1‑94 (MQ=60)

                                        GTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCG                                                   <  SRR4302535.2311215/94‑1 (MQ=60)

                                         TAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTACGCAAGCGCTGGCACAACAAAAATCGC                                                  >  SRR4302535.1350240/1‑94 (MQ=60)

                                          AACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCT                                                 <  SRR4302535.3497850/94‑1 (MQ=60)

                                           ACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTA                                                <  SRR4302535.4527183/94‑1 (MQ=60)

                                            CGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTAT                                               >  SRR4302535.1462639/1‑94 (MQ=60)

                                             GTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAGCAAAATTCGCTATC                                              <  SRR4302535.2408585/94‑1 (MQ=60)

                                             GTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATC                                              >  SRR4302535.1226717/1‑94 (MQ=60)

                                             GTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATC                                              >  SRR4302535.1315261/1‑94 (MQ=60)

                                             GTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATC                                              >  SRR4302535.3105532/1‑94 (MQ=60)

                                             GTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATC                                              <  SRR4302535.1556944/94‑1 (MQ=60)

                                             GTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATC                                              >  SRR4302535.2069376/1‑94 (MQ=60)

                                              TTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCT                                             <  SRR4302535.157469/94‑1 (MQ=60)

                                              TTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCT                                             <  SRR4302535.4156885/94‑1 (MQ=60)

                                               TCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTG                                            <  SRR4302535.851442/94‑1 (MQ=60)

                                                  CGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTG                                         <  SRR4302535.1181578/94‑1 (MQ=60)

                                                  CGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTG                                         <  SRR4302535.3639722/94‑1 (MQ=60)

                                                   GACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGG                                        >  SRR4302535.2916808/1‑94 (MQ=60)

                                                   GACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGG                                        <  SRR4302535.885684/94‑1 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      >  SRR4302535.1335141/1‑94 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      >  SRR4302535.2026318/1‑94 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      <  SRR4302535.2643700/94‑1 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      >  SRR4302535.1268860/1‑94 (MQ=60)

                                                     CTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTG                                      >  SRR4302535.2532008/1‑94 (MQ=60)

                                                      TTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGG                                     >  SRR4302535.1501968/1‑94 (MQ=60)

                                                       TCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGA                                    <  SRR4302535.289847/94‑1 (MQ=60)

                                                        CGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGAT                                   >  SRR4302535.1930511/1‑94 (MQ=60)

                                                         GATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATG                                  <  SRR4302535.144679/94‑1 (MQ=60)

                                                         GATGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATG                                  <  SRR4302535.2389866/94‑1 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                >  SRR4302535.3305715/1‑94 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                <  SRR4302535.2080714/94‑1 (MQ=60)

                                                           TGATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAG                                <  SRR4302535.3639068/94‑1 (MQ=60)

                                                            GATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGT                               >  SRR4302535.4302105/1‑94 (MQ=60)

                                                            GATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGT                               >  SRR4302535.3102785/1‑94 (MQ=60)

                                                             ATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGCGTA                              >  SRR4302535.3630288/1‑94 (MQ=60)

                                                             ATCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTA                              >  SRR4302535.2291158/1‑94 (MQ=60)

                                                              TCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTGGCTATCTGCTGGTGGATGAGTAT                             <  SRR4302535.3907918/94‑1 (MQ=60)

                                                              TCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAT                             >  SRR4302535.276692/1‑94 (MQ=60)

                                                              TCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAT                             <  SRR4302535.3542136/94‑1 (MQ=60)

                                                              TCTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTAT                             <  SRR4302535.66371/94‑1 (MQ=60)

                                                               CTGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATC                            <  SRR4302535.156702/94‑1 (MQ=60)

                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                           >  SRR4302535.4468234/1‑94 (MQ=60)

                                                                TGATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCA                           <  SRR4302535.2041858/94‑1 (MQ=60)

                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                          >  SRR4302535.643218/1‑94 (MQ=60)

                                                                 GATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAG                          <  SRR4302535.968099/94‑1 (MQ=60)

                                                                  ATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGG                         >  SRR4302535.302201/1‑94 (MQ=60)

                                                                  ATTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGG                         >  SRR4302535.4089735/1‑94 (MQ=60)

                                                                   TTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGA                        <  SRR4302535.3370837/94‑1 (MQ=60)

                                                                   TTTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGA                        <  SRR4302535.4475019/94‑1 (MQ=60)

                                                                    TTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGAC                       <  SRR4302535.1957473/94‑1 (MQ=60)

                                                                    TTTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGAC                       <  SRR4302535.3009675/94‑1 (MQ=60)

                                                                     TTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACA                      >  SRR4302535.352539/1‑94 (MQ=60)

                                                                     TTATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAATCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACA                      <  SRR4302535.1529893/94‑1 (MQ=60)

                                                                      TATTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACAC                     >  SRR4302535.1946882/1‑94 (MQ=60)

                                                                        TTGCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCA                   <  SRR4302535.2883203/94‑1 (MQ=60)

                                                                          GCCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAAC                 <  SRR4302535.3121927/94‑1 (MQ=60)

                                                                           CCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACA                <  SRR4302535.356787/94‑1 (MQ=60)

                                                                           CCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACA                >  SRR4302535.2879067/1‑94 (MQ=60)

                                                                           CCGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACA                >  SRR4302535.364212/1‑94 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC               >  SRR4302535.3889677/1‑94 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC               >  SRR4302535.1945972/1‑94 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC               >  SRR4302535.831576/1‑94 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC               >  SRR4302535.779848/1‑94 (MQ=60)

                                                                            CGACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACAC               <  SRR4302535.2272281/94‑1 (MQ=60)

                                                                             GACGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATTAGTATCAGGACACCAACACC              >  SRR4302535.415912/1‑94 (MQ=60)

                                                                               CGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAG            >  SRR4302535.2184429/1‑94 (MQ=60)

                                                                               CGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAG            <  SRR4302535.3780136/94‑1 (MQ=60)

                                                                               CGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAG            <  SRR4302535.3515233/94‑1 (MQ=60)

                                                                               CGTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAG            >  SRR4302535.164868/1‑94 (MQ=60)

                                                                                GTTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGC           >  SRR4302535.664523/1‑94 (MQ=60)

                                                                                 TTGCTGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCC          <  SRR4302535.535548/94‑1 (MQ=60)

                                                                                     TGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAACTTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTA      <  SRR4302535.4454511/94‑1 (MQ=60)

                                                                                     TGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTA      >  SRR4302535.2311642/1‑94 (MQ=60)

                                                                                     TGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTA      <  SRR4302535.1357368/94‑1 (MQ=60)

                                                                                     TGCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTA      >  SRR4302535.3880264/1‑94 (MQ=60)

                                                                                      GCTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTAT     <  SRR4302535.3099919/94‑1 (MQ=60)

                                                                                       CTGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATG    <  SRR4302535.4337703/94‑1 (MQ=60)

                                                                                        TGCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGA   >  SRR4302535.2811056/1‑94 (MQ=60)

                                                                                         GCAACGCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  <  SRR4302535.3344254/94‑1 (MQ=60)

GCCCATTGTTATGGGCTGTATGATGCACACCTGAAAGCCTGTAACGTTCTCGACTTCGATGATCTGATTTTATTGCCGACGTTGCTGCTGCAAGCCAATGAAGAAGTCCGCAAGCGCTGGCAGAACAAAATTCGCTATCTGCTGGTGGATGAGTATCAGGACACCAACACCAGCCAGTATGAG  >  NC_000913/3958784‑3958966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`