Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,960,578 2 bp→CG coding (1266‑1267/1485 nt) gppA ← guanosine pentaphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,960,5780GC100.0% 758.1 / NA 241L423V (CTC→GTC) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (111/130);  total (111/130)
*NC_0009133,960,5790CG100.0% 760.9 / NA 241R422R (CGG→CGC) gppAguanosine pentaphosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (111/130);  total (111/130)

Only 100 of 257 total aligned reads displayed.

GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT  >  NC_000913/3960491‑3960665
                                                                                       ||                                                                                      
gTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAcgcgcg                                                                                    >  1:2361162/1‑93 (MQ=255)
gTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAcgcgcg                                                                                    >  1:575125/1‑93 (MQ=255)
gTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAcgcgcg                                                                                    <  2:1345246/93‑1 (MQ=255)
gTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAcgcgcg                                                                                    >  2:3666326/1‑93 (MQ=255)
 tAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcgg                                                                                   >  2:127702/1‑93 (MQ=255)
  aaTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCgcggc                                                                                  <  1:1890879/93‑1 (MQ=255)
  aaTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGATGGTCTGGGACGCgcggc                                                                                  >  2:418696/1‑93 (MQ=255)
   aTGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATCGCCAGGCGTAGTAAACGGCAGAGGTGTTCTGCGACGCgcggcg                                                                                 >  1:2950871/1‑93 (MQ=255)
     gTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGt                                                                               <  2:3327146/93‑1 (MQ=255)
      tCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTa                                                                              <  1:326087/93‑1 (MQ=255)
      tCATCTCTGGCACGAGATAGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTa                                                                              <  2:3893831/93‑1 (MQ=255)
       cATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc                                                                             <  2:3587249/93‑1 (MQ=255)
       cATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAc                                                                             <  1:1811681/93‑1 (MQ=255)
         tctcTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACgg                                                                           >  1:4229344/1‑93 (MQ=255)
         tctcTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACgg                                                                           >  1:3111247/1‑93 (MQ=255)
          ctctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGc                                                                          >  1:2427941/1‑93 (MQ=255)
           tctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCa                                                                         >  2:1478544/1‑93 (MQ=255)
            ctGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAt                                                                        >  1:2899546/1‑93 (MQ=255)
             tGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAtt                                                                       <  1:930662/93‑1 (MQ=255)
             tGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAtt                                                                       <  2:1591297/93‑1 (MQ=255)
              ggCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAttt                                                                      <  2:641829/93‑1 (MQ=255)
                 aCGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTgt                                                                   >  2:246424/1‑92 (MQ=255)
                 aCGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGc                                                                   >  2:4122083/1‑93 (MQ=255)
                 aCGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGc                                                                   >  2:699146/1‑93 (MQ=255)
                  cGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCt                                                                  >  2:4496874/1‑93 (MQ=255)
                  cGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCt                                                                  >  1:3825062/1‑93 (MQ=255)
                    agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa                                                                >  1:3963540/1‑93 (MQ=255)
                    agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa                                                                <  2:806572/93‑1 (MQ=255)
                    agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa                                                                <  1:4500682/93‑1 (MQ=255)
                    agaTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGa                                                                <  2:2781344/93‑1 (MQ=255)
                      aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATg                                                              >  1:34813/1‑93 (MQ=255)
                      aTCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATg                                                              >  2:2228794/1‑93 (MQ=255)
                       tCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGc                                                             <  2:1260338/93‑1 (MQ=255)
                        cGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa                                                            <  2:1131138/93‑1 (MQ=255)
                        cGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCa                                                            <  2:2256268/93‑1 (MQ=255)
                         gTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAg                                                           >  1:4369739/1‑93 (MQ=255)
                          tCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGc                                                          <  1:4049988/93‑1 (MQ=255)
                          tCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGc                                                          >  2:4344374/1‑93 (MQ=255)
                          tCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGc                                                          <  2:2821394/93‑1 (MQ=255)
                            aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  2:3930228/93‑1 (MQ=255)
                            aCGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGa                                                        <  2:4462187/93‑1 (MQ=255)
                               gCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATga                                                     <  2:3805952/93‑1 (MQ=255)
                                 gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgaga                                                   >  2:468652/1‑93 (MQ=255)
                                 gACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgaga                                                   <  2:4099311/93‑1 (MQ=255)
                                   cGGCTGGCAAAAATGATGGCCAGTCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga                                                 <  2:2215746/93‑1 (MQ=255)
                                   cGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga                                                 <  1:493481/93‑1 (MQ=255)
                                   cGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATgagaga                                                 <  1:3405214/93‑1 (MQ=255)
                                    ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAt                                                <  2:1806195/93‑1 (MQ=255)
                                    ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAt                                                <  2:1401532/93‑1 (MQ=255)
                                    ggCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAt                                                <  1:1071105/93‑1 (MQ=255)
                                     gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               >  2:4162828/1‑93 (MQ=255)
                                     gCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATc                                               >  1:1499970/1‑93 (MQ=255)
                                       tGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGa                                             <  1:2066467/93‑1 (MQ=255)
                                         gCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAcc                                           >  1:1978141/1‑93 (MQ=255)
                                          cAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCg                                          <  2:1867579/93‑1 (MQ=255)
                                             aaaTGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGAt                                       <  1:1535846/93‑1 (MQ=255)
                                                tgatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAg                                    <  2:203418/93‑1 (MQ=255)
                                                 gatgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGt                                   <  2:3940851/93‑1 (MQ=255)
                                                   tgGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCGTTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCt                                 <  2:293808/93‑1 (MQ=255)
                                                    ggCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGGAGCGATGAGAGATCGACCGGATTAGTCTg                                >  1:2280346/1‑93 (MQ=255)
                                                    ggCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTg                                >  1:2535986/1‑93 (MQ=255)
                                                     gCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTgg                               <  1:2185169/93‑1 (MQ=255)
                                                      ccAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGt                              <  2:4041446/93‑1 (MQ=255)
                                                      ccAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGt                              <  1:1163301/93‑1 (MQ=255)
                                                       cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGtt                             >  2:2396549/1‑93 (MQ=255)
                                                       cAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGtt                             >  1:3828195/1‑93 (MQ=255)
                                                        aGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTg                            >  1:3012643/1‑93 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           <  2:1997046/93‑1 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           <  1:2852294/93‑1 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           <  1:1710174/93‑1 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           <  2:648973/93‑1 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           >  1:4590319/1‑93 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           >  2:2980270/1‑93 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           <  1:1123766/93‑1 (MQ=255)
                                                         ggCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGa                           >  2:3494084/1‑93 (MQ=255)
                                                            gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa                        >  1:3669450/1‑93 (MQ=255)
                                                            gtagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCa                        <  2:3297364/93‑1 (MQ=255)
                                                             tagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAg                       >  2:1175279/1‑93 (MQ=255)
                                                             tagtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAg                       >  1:4254926/1‑93 (MQ=255)
                                                               gtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTa                     <  1:1768615/93‑1 (MQ=255)
                                                               gtaAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTa                     <  2:1650653/93‑1 (MQ=255)
                                                                taAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAg                    >  1:3883052/1‑93 (MQ=255)
                                                                 aaaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGc                   <  1:25919/93‑1 (MQ=255)
                                                                  aaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCg                  >  2:942989/1‑93 (MQ=255)
                                                                  aaCGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCg                  >  2:2117452/1‑93 (MQ=255)
                                                                      gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGc              >  2:565314/1‑93 (MQ=255)
                                                                      gCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGc              >  2:3142219/1‑93 (MQ=255)
                                                                       cAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATTGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGcc             >  2:2153252/1‑93 (MQ=255)
                                                                       cAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGcc             <  2:3350733/93‑1 (MQ=255)
                                                                          agTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGc          >  2:265646/1‑93 (MQ=255)
                                                                           gttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCa         >  1:3522379/1‑93 (MQ=255)
                                                                            ttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCaa        <  1:811029/93‑1 (MQ=255)
                                                                            ttgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCaa        >  1:4460898/1‑93 (MQ=255)
                                                                             tgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAt       >  2:1098993/1‑93 (MQ=255)
                                                                             tgttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAt       >  1:2134859/1‑93 (MQ=255)
                                                                              gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtt      <  1:71158/93‑1 (MQ=255)
                                                                              gttCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtt      >  1:1589450/1‑93 (MQ=255)
                                                                                tCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtttt    >  1:1008032/1‑93 (MQ=255)
                                                                                tCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtttt    <  1:1246718/93‑1 (MQ=255)
                                                                                  tGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAtttttt  >  1:1388653/1‑93 (MQ=255)
                                                                                       ||                                                                                      
GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT  >  NC_000913/3960491‑3960665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  >  NC_000913/3960485‑3960667
                                                                                             |                                                                                         
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAC                                                                                           <  SRR4302535.3426077/94‑1 (MQ=60)
   CCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGA‑‑CGCG                                                                                      >  SRR4302535.3711931/1‑94 (MQ=60)
   CCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGAAGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGA‑‑CGCG                                                                                      >  SRR4302535.4668167/1‑94 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      >  SRR4302535.2378114/1‑94 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      >  SRR4302535.579162/1‑94 (MQ=60)
     TGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCG                                                                                      <  SRR4302535.507529/94‑1 (MQ=60)
      GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGG                                                                                     >  SRR4302535.1883170/1‑94 (MQ=60)
      GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGG                                                                                     <  SRR4302535.2569565/94‑1 (MQ=60)
      GTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGCCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGG                                                                                     <  SRR4302535.2569564/94‑1 (MQ=60)
        AATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCG                                                                                   <  SRR4302535.1904498/94‑1 (MQ=60)
        AATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATCGCCAGGCGTAGTAAACGGCAGAGGTGTTCTGCGACGCGCGGCG                                                                                   >  SRR4302535.2971600/1‑94 (MQ=60)
          TGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGT                                                                                 <  SRR4302535.4654269/94‑1 (MQ=60)
            TCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTAC                                                                               <  SRR4302535.328348/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              <  SRR4302535.1824780/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              <  SRR4302535.1114658/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              <  SRR4302535.2347209/94‑1 (MQ=60)
             CATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACG                                                                              >  SRR4302535.50834/1‑94 (MQ=60)
              ATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGG                                                                             >  SRR4302535.3132908/1‑94 (MQ=60)
              ATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGG                                                                             >  SRR4302535.2200491/1‑94 (MQ=60)
              ATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGG                                                                             >  SRR4302535.4258290/1‑94 (MQ=60)
               TCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGC                                                                            >  SRR4302535.1524470/1‑94 (MQ=60)
               TCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGC                                                                            >  SRR4302535.2445437/1‑94 (MQ=60)
                CTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCA                                                                           >  SRR4302535.2257956/1‑94 (MQ=60)
                CTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGGTGTTCTGCGACGCGCGGCGGTACGGCA                                                                           >  SRR4302535.1809119/1‑94 (MQ=60)
                CTCTGGCACGAGAGCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGGGGTACGGCA                                                                           <  SRR4302535.1570440/94‑1 (MQ=60)
                 TCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCAT                                                                          >  SRR4302535.2920047/1‑94 (MQ=60)
                   TGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTT                                                                        <  SRR4302535.937243/94‑1 (MQ=60)
                   TGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTT                                                                        >  SRR4302535.306078/1‑94 (MQ=60)
                      CACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGC                                                                     >  SRR4302535.592764/1‑94 (MQ=60)
                       ACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCT                                                                    >  SRR4302535.3851446/1‑94 (MQ=60)
                        CGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTG                                                                   <  SRR4302535.3516836/94‑1 (MQ=60)
                         GAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGA                                                                  >  SRR4302535.1754094/1‑94 (MQ=60)
                         GAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGA                                                                  >  SRR4302535.3990615/1‑94 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 <  SRR4302535.2441296/94‑1 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGAT                                                                 <  SRR4302535.4531592/94‑1 (MQ=60)
                          AGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGGTGTTCTTCGACGAGCGGGGGGACGGGATTTTGCTGAT                                                                 >  SRR4302535.1396309/1‑94 (MQ=60)
                           GATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATG                                                                <  SRR4302535.2907383/94‑1 (MQ=60)
                           GATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATG                                                                >  SRR4302535.35080/1‑94 (MQ=60)
                              CGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAG                                                             >  SRR4302535.4399644/1‑94 (MQ=60)
                               GTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGC                                                            <  SRR4302535.3219615/94‑1 (MQ=60)
                               GTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGC                                                            <  SRR4302535.1670632/94‑1 (MQ=60)
                                TCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCG                                                           <  SRR4302535.4064180/94‑1 (MQ=60)
                                TCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCG                                                           <  SRR4302535.4077715/94‑1 (MQ=60)
                                GCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCG                                                           <  SRR4302535.2760628/94‑1 (MQ=60)
                                 CACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGA                                                          <  SRR4302535.746880/94‑1 (MQ=60)
                                  ACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAT                                                         <  SRR4302535.4505581/94‑1 (MQ=60)
                                  ACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGAT                                                         >  SRR4302535.3585317/1‑94 (MQ=60)
                                     GCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTGTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAG                                                      <  SRR4302535.2529639/94‑1 (MQ=60)
                                     GCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAG                                                      <  SRR4302535.4279728/94‑1 (MQ=60)
                                       GACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAG                                                    >  SRR4302535.3727847/1‑94 (MQ=60)
                                         CGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAT                                                  <  SRR4302535.496940/94‑1 (MQ=60)
                                         CGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAT                                                  <  SRR4302535.3428910/94‑1 (MQ=60)
                                         CGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGGTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGAT                                                  >  SRR4302535.2155778/1‑94 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 <  SRR4302535.1078718/94‑1 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 <  SRR4302535.1792444/94‑1 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 >  SRR4302535.1510762/1‑94 (MQ=60)
                                          GGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATC                                                 <  SRR4302535.3898711/94‑1 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               >  SRR4302535.4354872/1‑94 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               <  SRR4302535.526857/94‑1 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               <  SRR4302535.143266/94‑1 (MQ=60)
                                            CTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGA                                               >  SRR4302535.1910930/1‑94 (MQ=60)
                                             TGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGAC                                              <  SRR4302535.2081366/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302535.3433544/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302535.2970554/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             >  SRR4302535.1992387/1‑94 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302535.3986480/94‑1 (MQ=60)
                                              GGCAAAAATGATGGCCAGGCGTACTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACC                                             <  SRR4302535.156640/94‑1 (MQ=60)
                                                 AAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGA                                          <  SRR4302535.970552/94‑1 (MQ=60)
                                                   AAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATT                                        >  SRR4302535.2138455/1‑94 (MQ=60)
                                                   AAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATT                                        <  SRR4302535.1546912/94‑1 (MQ=60)
                                                   AAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATT                                        <  SRR4302535.2864024/94‑1 (MQ=60)
                                                    AATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTA                                       >  SRR4302535.3438453/1‑94 (MQ=60)
                                                      TGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGT                                     <  SRR4302535.796553/94‑1 (MQ=60)
                                                       GATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTC                                    >  SRR4302535.1616489/1‑94 (MQ=60)
                                                        ATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCT                                   <  SRR4302535.2005520/94‑1 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGGAGCGATGAGAGATCGACCGGATTAGTCTG                                  >  SRR4302535.2296636/1‑94 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  <  SRR4302535.3155685/94‑1 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  >  SRR4302535.2554252/1‑94 (MQ=60)
                                                         TGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTG                                  <  SRR4302535.4578577/94‑1 (MQ=60)
                                                          GGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGG                                 <  SRR4302535.440434/94‑1 (MQ=60)
                                                           GCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGT                                <  SRR4302535.2200745/94‑1 (MQ=60)
                                                            CCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTT                               >  SRR4302535.3854602/1‑94 (MQ=60)
                                                            CCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTT                               <  SRR4302535.1171611/94‑1 (MQ=60)
                                                             CAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTG                              >  SRR4302535.2334344/1‑94 (MQ=60)
                                                             CAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTG                              >  SRR4302535.4219599/1‑94 (MQ=60)
                                                             CAGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTG                              >  SRR4302535.3033754/1‑94 (MQ=60)
                                                              AGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGCGAGATCGACCGGATTAGTCTGGgtgg                             >  SRR4302535.1087050/1‑90 (MQ=60)
                                                              AGGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGA                             >  SRR4302535.4621564/1‑94 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            <  SRR4302535.1722538/94‑1 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            <  SRR4302535.1131785/94‑1 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            <  SRR4302535.4197984/94‑1 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            <  SRR4302535.2872501/94‑1 (MQ=60)
                                                               GGCGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTCGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAG                            <  SRR4302535.3322515/94‑1 (MQ=60)
                                                                 CGTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCA                          >  SRR4302535.3694862/1‑94 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         >  SRR4302535.4284075/1‑94 (MQ=60)
                                                                  GTAGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAG                         >  SRR4302535.3903124/1‑94 (MQ=60)
                                                                    AGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA                       <  SRR4302535.490084/94‑1 (MQ=60)
                                                                    AGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA                       >  SRR4302535.3023426/1‑94 (MQ=60)
                                                                    AGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA                       >  SRR4302535.3852413/1‑94 (MQ=60)
                                                                    AGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA                       <  SRR4302535.1946724/94‑1 (MQ=60)
                                                                    AGTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTA                       >  SRR4302535.2896159/1‑94 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      >  SRR4302535.3909692/1‑94 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      <  SRR4302535.1242726/94‑1 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      <  SRR4302535.1781437/94‑1 (MQ=60)
                                                                     GTAAACGGCAGAGTTGTCCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAG                      <  SRR4302535.4291789/94‑1 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     <  SRR4302535.1053103/94‑1 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     <  SRR4302535.4496284/94‑1 (MQ=60)
                                                                      TAAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGC                     >  SRR4302535.2976907/1‑94 (MQ=60)
                                                                       AAACGGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCG                    <  SRR4302535.26120/94‑1 (MQ=60)
                                                                           GGCAGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGC                >  SRR4302535.211617/1‑94 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             <  SRR4302535.1145281/94‑1 (MQ=60)
                                                                              AGAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAG             <  SRR4302535.4059827/94‑1 (MQ=60)
                                                                               GAGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCCGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGC            >  SRR4302535.2534055/1‑94 (MQ=60)
                                                                                AGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCA           >  SRR4302535.3546842/1‑94 (MQ=60)
                                                                                AGTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCA           >  SRR4302535.830961/1‑94 (MQ=60)
                                                                                 GTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAA          >  SRR4302535.4491493/1‑94 (MQ=60)
                                                                                 GTTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAA          >  SRR4302535.1481636/1‑94 (MQ=60)
                                                                                  TTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAT         >  SRR4302535.2150241/1‑94 (MQ=60)
                                                                                  TTGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAAT         <  SRR4302535.816697/94‑1 (MQ=60)
                                                                                   TGTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATT        >  SRR4302535.1600923/1‑94 (MQ=60)
                                                                                    GTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTT       >  SRR4302535.3835714/1‑94 (MQ=60)
                                                                                    GTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTT       >  SRR4302535.3546155/1‑94 (MQ=60)
                                                                                    GTTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTT       <  SRR4302535.71680/94‑1 (MQ=60)
                                                                                     TTCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTT      >  SRR4302535.1015195/1‑94 (MQ=60)
                                                                                      TCTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTT     <  SRR4302535.1255613/94‑1 (MQ=60)
                                                                                       CTGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTT    >  SRR4302535.1398566/1‑94 (MQ=60)
                                                                                        TGCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTC   <  SRR4302535.1184936/94‑1 (MQ=60)
                                                                                         GCGACGCGCGGCGGTACGGCATTTTTCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  <  SRR4302535.4559305/94‑1 (MQ=60)
                                                                                         GCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  <  SRR4302535.2582535/94‑1 (MQ=60)
                                                                                         GCGACGCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  <  SRR4302535.3997116/94‑1 (MQ=60)
                                                                                             |                                                                                         
TAGCCTGTAATGTCATCTCTGGCACGAGATCGTCACGGCGACGGCTGGCAAAAATGATGGCCAGGCGTAGTAAACGGCAGAGTTGTTCTGCGAGCCGCGGCGGTACGGCATTTTGCTGATGCAGCGATGAGAGATCGACCGGATTAGTCTGGTTGAGCAGTAGCGTCGCCAGCAATTTTTTCT  >  NC_000913/3960485‑3960667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑