Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,992	A→G	E117G (GAG→GGG)	wzzE →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,966,992	0	A	G	100.0%	700.7 / NA	222	E117G (GAG→GGG)	wzzE	putative transport protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (116/106); total (116/106)

Only 100 of 237 total aligned reads displayed.

CAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACA  >  NC_000913/3966902‑3967081

cAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGt                                                                                         <  1:3333365/93‑1 (MQ=255)

cAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGt                                                                                         >  1:4642361/1‑93 (MQ=255)

  aaCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTc                                                                                       >  1:384999/1‑93 (MQ=255)

   aCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCt                                                                                      <  2:439253/93‑1 (MQ=255)

   aCATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCt                                                                                      <  1:322581/93‑1 (MQ=255)

    cATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTg                                                                                     <  1:3663949/93‑1 (MQ=255)

     aTGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTgg                                                                                    <  1:425561/93‑1 (MQ=255)

      tGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTTTAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGc                                                                                   <  1:3103220/93‑1 (MQ=255)

      tGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGGTCTGGc                                                                                   >  1:42882/1‑93 (MQ=255)

       ggCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCt                                                                                  >  2:2625224/1‑93 (MQ=255)

         cTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGc                                                                                >  1:770562/1‑93 (MQ=255)

          ttCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCa                                                                               >  1:1934245/1‑93 (MQ=255)

             tGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAc                                                                            >  2:3437699/1‑93 (MQ=255)

              gCCGACCAACCATCGGTCATGGACGAAGCCTCTAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAcc                                                                           >  2:3659128/1‑93 (MQ=255)

              gCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGGAAAcc                                                                           >  1:2057465/1‑93 (MQ=255)

                 gACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATGCCCGCAGAGGGTTCTGGCTGCAAACCGAc                                                                        <  1:528591/93‑1 (MQ=255)

                 gACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGAc                                                                        <  1:2925534/93‑1 (MQ=255)

                  accaaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACt                                                                       <  1:4325882/93‑1 (MQ=255)

                   ccaaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTa                                                                      >  1:4048488/1‑93 (MQ=255)

                    caaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTAt                                                                     <  2:2121378/93‑1 (MQ=255)

                     aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTAtt                                                                    >  2:2887596/1‑93 (MQ=255)

                     aaccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTAtt                                                                    >  1:150604/1‑93 (MQ=255)

                      accaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTa                                                                   <  1:2665191/93‑1 (MQ=255)

                       ccaTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTAc                                                                  <  1:4479304/93‑1 (MQ=255)

                        caTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACa                                                                 <  2:107369/93‑1 (MQ=255)

                         aTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACaa                                                                >  2:1400681/1‑93 (MQ=255)

                         aTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACaa                                                                >  1:3556153/1‑93 (MQ=255)

                         aTCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACaa                                                                >  2:961860/1‑93 (MQ=255)

                          tCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACaaa                                                               <  1:864856/93‑1 (MQ=255)

                           cGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAAc                                                              <  1:1019000/93‑1 (MQ=255)

                            ggTCAGGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACa                                                             <  1:4143881/93‑1 (MQ=255)

                             gTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAg                                                            >  1:4062193/1‑93 (MQ=255)

                              tCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGc                                                           >  1:3188315/1‑93 (MQ=255)

                              tCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGc                                                           >  1:2165315/1‑93 (MQ=255)

                              tCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGc                                                           >  1:2251580/1‑93 (MQ=255)

                               cATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCg                                                          <  1:2459709/93‑1 (MQ=255)

                               cATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCg                                                          <  1:2597757/93‑1 (MQ=255)

                                aTGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCgg                                                         >  1:982715/1‑93 (MQ=255)

                                  ggACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAt                                                       <  1:1047118/93‑1 (MQ=255)

                                  ggACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAt                                                       <  1:3092491/93‑1 (MQ=255)

                                   gACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtg                                                      >  2:4179831/1‑93 (MQ=255)

                                   gACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtg                                                      <  2:1668905/93‑1 (MQ=255)

                                   gACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtg                                                      >  1:625213/1‑93 (MQ=255)

                                     cGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtggt                                                    <  2:2304051/93‑1 (MQ=255)

                                      gAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtggtg                                                   >  2:1884949/1‑93 (MQ=255)

                                       aaGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAtggtgg                                                  <  1:3610318/93‑1 (MQ=255)

                                        aGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTggg                                                 >  1:1417423/1‑93 (MQ=255)

                                            tataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAAc                                             >  2:2759788/1‑93 (MQ=255)

                                            tataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAAc                                             >  1:1278706/1‑93 (MQ=255)

                                            tataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAAc                                             >  2:4021411/1‑93 (MQ=255)

                                             ataAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACa                                            >  2:3039405/1‑93 (MQ=255)

                                              taAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAg                                           >  1:72099/1‑93 (MQ=255)

                                              taAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAg                                           >  2:4299928/1‑93 (MQ=255)

                                              taAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAg                                           >  2:910873/1‑93 (MQ=255)

                                              taAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAg                                           >  1:462475/1‑93 (MQ=255)

                                               aaaGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGc                                          >  2:3561970/1‑93 (MQ=255)

                                                    gTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGc                                     >  2:74490/1‑93 (MQ=255)

                                                    gTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGc                                     <  1:1588080/93‑1 (MQ=255)

                                                         ttATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATg                                <  2:3261976/93‑1 (MQ=255)

                                                         ttATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATg                                <  2:2228544/93‑1 (MQ=255)

                                                          tATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgc                               <  2:48677/93‑1 (MQ=255)

                                                          tATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgc                               <  1:3734906/93‑1 (MQ=255)

                                                          tATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgc                               <  2:3467179/93‑1 (MQ=255)

                                                           aTGCAACTGGCCTCGTGGGATACCCGCAGAGGTTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgcg                              <  2:3803735/93‑1 (MQ=255)

                                                            tGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgcgg                             <  2:4540759/93‑1 (MQ=255)

                                                            tGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgcgg                             <  1:377312/93‑1 (MQ=255)

                                                              cAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATgcggcg                           <  2:3714971/93‑1 (MQ=255)

                                                               aaCTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGt                          <  2:4110793/93‑1 (MQ=255)

                                                               aaCTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGt                          <  1:2221363/93‑1 (MQ=255)

                                                                aCTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGtt                         <  1:2528149/93‑1 (MQ=255)

                                                                 cTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTg                        >  2:3315537/1‑93 (MQ=255)

                                                                  tGGCCTCGTGGTATACCCGCAGACGCTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGc                       <  2:1849703/93‑1 (MQ=255)

                                                                  tGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGc                       >  2:57815/1‑93 (MQ=255)

                                                                   ggCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCt                      >  1:1760612/1‑93 (MQ=255)

                                                                      cTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGa                   <  2:2374932/93‑1 (MQ=255)

                                                                      cTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGa                   <  2:1379323/93‑1 (MQ=255)

                                                                      cTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGa                   <  1:271981/93‑1 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                  <  2:2020681/93‑1 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                  <  1:1360642/93‑1 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                  >  2:3883310/1‑93 (MQ=255)

                                                                       tCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAt                  <  1:2089106/93‑1 (MQ=255)

                                                                         gTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGa                >  1:3077301/1‑93 (MQ=255)

                                                                          tGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaa               >  1:1601581/1‑93 (MQ=255)

                                                                          tGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaa               <  2:101189/93‑1 (MQ=255)

                                                                          tGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGaa               <  2:3839760/93‑1 (MQ=255)

                                                                            ggATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAAt             <  2:2722834/93‑1 (MQ=255)

                                                                            ggATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAAt             >  2:3325410/1‑93 (MQ=255)

                                                                              atccccGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa           <  2:1923956/90‑1 (MQ=255)

                                                                              aTACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGa           >  1:3520098/1‑93 (MQ=255)

                                                                               tACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGCAATGAt          >  1:1862654/1‑93 (MQ=255)

                                                                               tACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAt          >  2:631489/1‑93 (MQ=255)

                                                                                ccccGCAGAGGGTTCTGGCTGCAAACCGCCTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt         <  2:2839976/92‑1 (MQ=255)

                                                                                aCCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt         <  2:3098677/93‑1 (MQ=255)

                                                                                aCCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGAtt         <  1:887516/93‑1 (MQ=255)

                                                                                   cGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaac      <  2:407911/93‑1 (MQ=255)

                                                                                   cGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaac      <  2:2199307/93‑1 (MQ=255)

                                                                                    gCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaaca     <  2:1754156/93‑1 (MQ=255)

                                                                                     cAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaacaa    <  2:2970007/93‑1 (MQ=255)

                                                                                      agagggTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTaacaac   <  1:3071501/93‑1 (MQ=255)

                                                                                       gagggTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAacaaca  >  1:413618/1‑93 (MQ=255)

CAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACA  >  NC_000913/3966902‑3967081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCC  >  NC_000913/3966899‑3967084

GTTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGG                                                                                              >  SRR4302535.4521708/1‑94 (MQ=60)

GTTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGG                                                                                              <  SRR4302535.994362/94‑1 (MQ=60)

 TTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGG                                                                                             >  SRR4302535.1362533/1‑94 (MQ=60)

 TTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGG                                                                                             <  SRR4302535.3904751/94‑1 (MQ=60)

  TCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGT                                                                                            >  SRR4302535.4673828/1‑94 (MQ=60)

  TCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGT                                                                                            >  SRR4302535.3648777/1‑94 (MQ=60)

  TCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGT                                                                                            <  SRR4302535.1178887/94‑1 (MQ=60)

   CAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTT                                                                                           <  SRR4302535.3356565/94‑1 (MQ=60)

    AAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTC                                                                                          >  SRR4302535.387670/1‑94 (MQ=60)

    AAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTC                                                                                          >  SRR4302535.27036/1‑94 (MQ=60)

    AAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTC                                                                                          <  SRR4302535.2763373/94‑1 (MQ=60)

     AACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCT                                                                                         <  SRR4302535.4311826/94‑1 (MQ=60)

      ACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTG                                                                                        <  SRR4302535.324813/94‑1 (MQ=60)

      ACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTG                                                                                        <  SRR4302535.3779054/94‑1 (MQ=60)

       CATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGG                                                                                       <  SRR4302535.3689313/94‑1 (MQ=60)

        ATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGC                                                                                      <  SRR4302535.2482530/94‑1 (MQ=60)

        ATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGC                                                                                      <  SRR4302535.428527/94‑1 (MQ=60)

        ATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGC                                                                                      >  SRR4302535.1556999/1‑94 (MQ=60)

        ATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGGTCTGGC                                                                                      >  SRR4302535.43206/1‑94 (MQ=60)

         TGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTTTAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCT                                                                                     <  SRR4302535.3124823/94‑1 (MQ=60)

          GGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTG                                                                                    >  SRR4302535.2547145/1‑94 (MQ=60)

           GCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGC                                                                                   >  SRR4302535.775986/1‑94 (MQ=60)

           GCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGC                                                                                   >  SRR4302535.2532561/1‑94 (MQ=60)

            CTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCA                                                                                  >  SRR4302535.4022376/1‑94 (MQ=60)

            CTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCA                                                                                  >  SRR4302535.1948175/1‑94 (MQ=60)

               CTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAAC                                                                               <  SRR4302535.564591/94‑1 (MQ=60)

                TGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGGAAACC                                                                              >  SRR4302535.2072287/1‑94 (MQ=60)

                TGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACC                                                                              >  SRR4302535.471907/1‑94 (MQ=60)

                TGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACA                                                                              >  SRR4302535.4666383/1‑94 (MQ=60)

                   CGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGAC                                                                           >  SRR4302535.3997911/1‑94 (MQ=60)

                    GACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATGCCCGCAGAGGGTTCTGGCTGCAAACCGACT                                                                          <  SRR4302535.532285/94‑1 (MQ=60)

                    GACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACT                                                                          <  SRR4302535.2946157/94‑1 (MQ=60)

                     ACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTA                                                                         >  SRR4302535.4076199/1‑94 (MQ=60)

                     ACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTA                                                                         <  SRR4302535.4355463/94‑1 (MQ=60)

                      CCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTAT                                                                        >  SRR4302535.992243/1‑94 (MQ=60)

                       CAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCGAACCGACTATT                                                                       >  SRR4302535.4088374/1‑94 (MQ=60)

                       CAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATT                                                                       >  SRR4302535.151656/1‑94 (MQ=60)

                        AACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTA                                                                      >  SRR4302535.297592/1‑94 (MQ=60)

                        AACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTA                                                                      <  SRR4302535.1603214/94‑1 (MQ=60)

                         ACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTAC                                                                     <  SRR4302535.2684274/94‑1 (MQ=60)

                         ACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTAC                                                                     >  SRR4302535.2650394/1‑94 (MQ=60)

                         ACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTAC                                                                     >  SRR4302535.4281669/1‑94 (MQ=60)

                          CCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACA                                                                    <  SRR4302535.4510055/94‑1 (MQ=60)

                           CATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAA                                                                   >  SRR4302535.2265591/1‑94 (MQ=60)

                           CATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAA                                                                   >  SRR4302535.3580818/1‑94 (MQ=60)

                            ATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAA                                                                  >  SRR4302535.1816226/1‑94 (MQ=60)

                             TCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAAC                                                                 <  SRR4302535.870968/94‑1 (MQ=60)

                              CGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACA                                                                <  SRR4302535.1026238/94‑1 (MQ=60)

                               GGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAG                                                               >  SRR4302535.4089996/1‑94 (MQ=60)

                               GGTCAGGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAG                                                               <  SRR4302535.4172256/94‑1 (MQ=60)

                                GTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGC                                                              >  SRR4302535.3210363/1‑94 (MQ=60)

                                GTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGC                                                              >  SRR4302535.2267614/1‑94 (MQ=60)

                                GTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGC                                                              >  SRR4302535.2180841/1‑94 (MQ=60)

                                GTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGC                                                              >  SRR4302535.763104/1‑94 (MQ=60)

                                  CATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGG                                                            <  SRR4302535.2616415/94‑1 (MQ=60)

                                  CATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGG                                                            >  SRR4302535.989697/1‑94 (MQ=60)

                                  CATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGG                                                            >  SRR4302535.1962401/1‑94 (MQ=60)

                                  CATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGG                                                            <  SRR4302535.2477444/94‑1 (MQ=60)

                                    TGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAT                                                          <  SRR4302535.1494580/94‑1 (MQ=60)

                                    TGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAT                                                          <  SRR4302535.130686/94‑1 (MQ=60)

                                    TGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGAT                                                          >  SRR4302535.3231886/1‑94 (MQ=60)

                                     GGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATG                                                         >  SRR4302535.629561/1‑94 (MQ=60)

                                     GGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATG                                                         <  SRR4302535.1054543/94‑1 (MQ=60)

                                     GGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATG                                                         <  SRR4302535.3114026/94‑1 (MQ=60)

                                      GACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGG                                                        <  SRR4302535.3593868/94‑1 (MQ=60)

                                      GACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGG                                                        >  SRR4302535.1033132/1‑94 (MQ=60)

                                        CGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTG                                                      >  SRR4302535.3114217/1‑94 (MQ=60)

                                        CGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTG                                                      <  SRR4302535.3546026/94‑1 (MQ=60)

                                          AAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGG                                                    <  SRR4302535.3635355/94‑1 (MQ=60)

                                          AAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGG                                                    >  SRR4302535.1403319/1‑94 (MQ=60)

                                          AAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGG                                                    >  SRR4302535.1427544/1‑94 (MQ=60)

                                          AAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGG                                                    >  SRR4302535.3116063/1‑94 (MQ=60)

                                           AGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGC                                                   >  SRR4302535.3703656/1‑94 (MQ=60)

                                             CCTATAAAGAGTTTGTTATGTAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTACAAACCGACTATTACAAACAGCGGATattggggca                                                 >  SRR4302535.4285814/1‑85 (MQ=60)

                                             CCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAA                                                 >  SRR4302535.3291380/1‑94 (MQ=60)

                                              CTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAAC                                                >  SRR4302535.1287836/1‑94 (MQ=60)

                                               TATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACA                                               >  SRR4302535.2083339/1‑94 (MQ=60)

                                                ATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAG                                              >  SRR4302535.1913508/1‑94 (MQ=60)

                                                ATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAG                                              >  SRR4302535.465706/1‑94 (MQ=60)

                                                ATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAG                                              >  SRR4302535.72625/1‑94 (MQ=60)

                                                 TAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGC                                             >  SRR4302535.2028010/1‑94 (MQ=60)

                                                 TAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGC                                             <  SRR4302535.4168766/94‑1 (MQ=60)

                                                  AAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCA                                            >  SRR4302535.456184/1‑94 (MQ=60)

                                                  AAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTACAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCA                                            <  SRR4302535.3410318/94‑1 (MQ=60)

                                                   AAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAA                                           >  SRR4302535.1619290/1‑94 (MQ=60)

                                                     GAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAG                                         <  SRR4302535.1565759/94‑1 (MQ=60)

                                                       GTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCC                                       <  SRR4302535.1599543/94‑1 (MQ=60)

                                                        TTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCG                                      >  SRR4302535.329601/1‑94 (MQ=60)

                                                         TTGTTATGCAACTGTCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGA                                     <  SRR4302535.873918/94‑1 (MQ=60)

                                                         TTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGA                                     >  SRR4302535.1171724/1‑94 (MQ=60)

                                                           GTTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATG                                   <  SRR4302535.2340880/94‑1 (MQ=60)

                                                            TTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAGAGCCGATGC                                  >  SRR4302535.676152/1‑94 (MQ=60)

                                                            TTATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGC                                  >  SRR4302535.4147022/1‑94 (MQ=60)

                                                             TATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCG                                 >  SRR4302535.3094987/1‑94 (MQ=60)

                                                             TATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCG                                 <  SRR4302535.3760754/94‑1 (MQ=60)

                                                              ATGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGG                                >  SRR4302535.755516/1‑94 (MQ=60)

                                                               tgtcactgagCTCGTGGGATGCCCGCAGCGGGTTCGGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGC                               <  SRR4302535.2294215/84‑1 (MQ=60)

                                                               TGCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGC                               <  SRR4302535.379931/94‑1 (MQ=60)

                                                                GCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCG                              <  SRR4302535.976471/94‑1 (MQ=60)

                                                                GCAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCG                              >  SRR4302535.3156783/1‑94 (MQ=60)

                                                                 CAACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGT                             >  SRR4302535.3080288/1‑94 (MQ=60)

                                                                  AACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTT                            <  SRR4302535.2237179/94‑1 (MQ=60)

                                                                   ACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTG                           <  SRR4302535.3705133/94‑1 (MQ=60)

                                                                   ACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTG                           <  SRR4302535.4607147/94‑1 (MQ=60)

                                                                   ACTGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTG                           <  SRR4302535.2546361/94‑1 (MQ=60)

                                                                     TGGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCT                         >  SRR4302535.1773360/1‑94 (MQ=60)

                                                                      GGCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTG                        <  SRR4302535.3109315/94‑1 (MQ=60)

                                                                       GCCTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGG                       <  SRR4302535.2174089/94‑1 (MQ=60)

                                                                         CTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAT                     >  SRR4302535.2192885/1‑94 (MQ=60)

                                                                         CTCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGAT                     <  SRR4302535.273872/94‑1 (MQ=60)

                                                                          TCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATG                    <  SRR4302535.1370373/94‑1 (MQ=60)

                                                                          TCGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATG                    <  SRR4302535.2104159/94‑1 (MQ=60)

                                                                           CGTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGA                   >  SRR4302535.3098768/1‑94 (MQ=60)

                                                                            GTGGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAA                  >  SRR4302535.1613137/1‑94 (MQ=60)

                                                                              GGGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAAT                <  SRR4302535.943182/94‑1 (MQ=60)

                                                                               GGATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATG               >  SRR4302535.3858514/1‑94 (MQ=60)

                                                                                GATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGA              >  SRR4302535.3544549/1‑94 (MQ=60)

                                                                                 ATACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGCAATGAT             >  SRR4302535.1876086/1‑94 (MQ=60)

                                                                                  TACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATT            <  SRR4302535.4342137/94‑1 (MQ=60)

                                                                                   ACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTA           >  SRR4302535.1793963/1‑94 (MQ=60)

                                                                                   ACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTA           >  SRR4302535.4110820/1‑94 (MQ=60)

                                                                                   ACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTA           <  SRR4302535.893800/94‑1 (MQ=60)

                                                                                   ACCCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTA           >  SRR4302535.791667/1‑94 (MQ=60)

                                                                                     CCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAAC         >  SRR4302535.3569753/1‑94 (MQ=60)

                                                                                     CCGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAAC         <  SRR4302535.1356516/94‑1 (MQ=60)

                                                                                      CGCAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACA        >  SRR4302535.2634009/1‑94 (MQ=60)

                                                                                        CAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAAC      <  SRR4302535.4371640/94‑1 (MQ=60)

                                                                                        CAGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAAC      >  SRR4302535.529033/1‑94 (MQ=60)

                                                                                         AGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACA     <  SRR4302535.3092947/94‑1 (MQ=60)

                                                                                         AGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACA     >  SRR4302535.2197572/1‑94 (MQ=60)

                                                                                         AGAGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACA     >  SRR4302535.416523/1‑94 (MQ=60)

                                                                                           AGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATC   >  SRR4302535.236054/1‑94 (MQ=60)

                                                                                           AGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATC   <  SRR4302535.1783982/94‑1 (MQ=60)

                                                                                           AGGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATC   <  SRR4302535.1080314/94‑1 (MQ=60)

                                                                                            GGGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCC  <  SRR4302535.1323996/94‑1 (MQ=60)

GTTCAAACATGGCTTCTGCCGACCAACCATCGGTCATGGACGAAGCCTATAAAGAGTTTGTTATGCAACTGGCCTCGTGGGATACCCGCAGAGAGTTCTGGCTGCAAACCGACTATTACAAACAGCGGATGGTGGGCAACAGCAAAGCCGATGCGGCGTTGCTGGATGAAATGATTAACAACATCC  >  NC_000913/3966899‑3967084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`