Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,967,307	A→G	D222G (GAC→GGC)	wzzE →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,967,307	0	A	G	99.6%	755.5 / NA	239	D222G (GAC→GGC)	wzzE	putative transport protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (97/141); minor base C (0/1); total (97/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

ATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAAT  >  NC_000913/3967217‑3967396

atgatgAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGcc                                                                                         >  2:441854/1‑93 (MQ=255)

atgatgAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGcc                                                                                         >  2:573476/1‑93 (MQ=255)

atgatgAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGcc                                                                                         >  2:2758728/1‑93 (MQ=255)

atgatgAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGcc                                                                                         <  2:2013504/93‑1 (MQ=255)

atgatgAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCAACGGcc                                                                                         >  2:2720632/1‑93 (MQ=255)

 tgatgaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGccg                                                                                        >  1:2281849/1‑93 (MQ=255)

   atgaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATATACGgccgcc                                                                                      >  2:4108092/1‑93 (MQ=255)

    tgaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGccgccg                                                                                     >  1:2872655/1‑93 (MQ=255)

     gaGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCgg                                                                                    <  2:3077301/93‑1 (MQ=255)

        cTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATg                                                                                 >  2:845742/1‑93 (MQ=255)

          gAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGaa                                                                               >  2:2095212/1‑93 (MQ=255)

            aaGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACa                                                                             <  2:4174587/93‑1 (MQ=255)

             aGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAg                                                                            <  1:126477/93‑1 (MQ=255)

               gcgcATGGNCNGCGCGTACCATCCNGNTGNAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGGTGAACAGCa                                                                          <  2:858370/93‑1 (MQ=255)

                cgcATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCAt                                                                         <  1:964483/93‑1 (MQ=255)

                cgcATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCAt                                                                         <  1:3550723/93‑1 (MQ=255)

                cgcATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCAt                                                                         <  1:3659128/93‑1 (MQ=255)

                 gcATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCAtt                                                                        <  2:3157627/93‑1 (MQ=255)

                  cATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTg                                                                       >  1:894364/1‑93 (MQ=255)

                  cATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTg                                                                       >  1:2994583/1‑93 (MQ=255)

                  cATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTg                                                                       >  1:4478165/1‑93 (MQ=255)

                  cATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTg                                                                       <  1:1859996/93‑1 (MQ=255)

                   aTGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGa                                                                      >  2:2670127/1‑93 (MQ=255)

                    tGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAg                                                                     <  2:4002085/93‑1 (MQ=255)

                    tGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAg                                                                     >  2:2887644/1‑93 (MQ=255)

                     gggCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGc                                                                    <  1:1167299/93‑1 (MQ=255)

                     gggCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGc                                                                    <  1:2470658/93‑1 (MQ=255)

                      ggcggcGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCa                                                                   <  1:3325410/93‑1 (MQ=255)

                      ggcggcGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGGGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTAAGCa                                                                   >  1:1011779/1‑93 (MQ=255)

                       gcggcgCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAg                                                                  <  2:2580391/93‑1 (MQ=255)

                        cggcgCGTCCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCGGGATGAACAGCATTGAACAgg                                                                 <  2:671466/93‑1 (MQ=255)

                        cggcgCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAgg                                                                 <  1:2355446/93‑1 (MQ=255)

                        cggcgCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAgg                                                                 <  1:2328491/93‑1 (MQ=255)

                           cgcgTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGgcgc                                                              <  1:2798117/93‑1 (MQ=255)

                            gcgTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCt                                                             <  1:1228064/93‑1 (MQ=255)

                             cgTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTg                                                            <  2:757773/93‑1 (MQ=255)

                               tACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGaa                                                          <  2:3134961/93‑1 (MQ=255)

                                aCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGaaa                                                         >  2:100305/1‑93 (MQ=255)

                                aCCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGaaa                                                         >  1:2959414/1‑93 (MQ=255)

                                 ccATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGaaaa                                                        <  1:3164398/93‑1 (MQ=255)

                                 ccATCAAGATGAAAGCTAAGATGAAGCGTCAGGAAGAGGTGTCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGaaaa                                                        <  2:2475110/93‑1 (MQ=255)

                                  cATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAt                                                       <  1:1444486/93‑1 (MQ=255)

                                  cATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAt                                                       <  1:675739/93‑1 (MQ=255)

                                  cATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAt                                                       <  2:1041529/93‑1 (MQ=255)

                                   aTCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAtt                                                      >  2:1571443/1‑93 (MQ=255)

                                   aTCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAtt                                                      >  2:3308142/1‑93 (MQ=255)

                                   aTCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAtt                                                      <  1:4299928/93‑1 (MQ=255)

                                    tCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGTCGCTGAAAATTg                                                     >  1:4075911/1‑93 (MQ=255)

                                     ccAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGc                                                    <  1:1338853/93‑1 (MQ=255)

                                       aGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTg                                                  >  2:4239053/1‑93 (MQ=255)

                                       aGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTg                                                  >  2:595550/1‑93 (MQ=255)

                                        gATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGa                                                 >  1:479269/1‑93 (MQ=255)

                                        gATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGa                                                 >  1:3480674/1‑93 (MQ=255)

                                        gATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGa                                                 <  2:4060580/93‑1 (MQ=255)

                                          tGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagc                                               >  1:1181006/1‑93 (MQ=255)

                                            aaaGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagcag                                             <  2:2244650/93‑1 (MQ=255)

                                             aaGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagcagc                                            <  2:1393370/93‑1 (MQ=255)

                                             aaGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGagcagc                                            >  2:354580/1‑93 (MQ=255)

                                              aGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAgcagca                                           >  2:3805593/1‑93 (MQ=255)

                                               gCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCat                                          <  2:625213/93‑1 (MQ=255)

                                                cTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCata                                         >  2:1664703/1‑93 (MQ=255)

                                                 tCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataa                                        <  1:394922/93‑1 (MQ=255)

                                                   aGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataata                                      <  1:1050980/93‑1 (MQ=255)

                                                   aGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataata                                      <  1:2061206/93‑1 (MQ=255)

                                                   aGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCataata                                      <  2:706000/93‑1 (MQ=255)

                                                    ggTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAatat                                     >  1:4595135/1‑93 (MQ=255)

                                                    ggTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAatat                                     >  1:2540691/1‑93 (MQ=255)

                                                     gTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAtt                                    <  2:2000260/93‑1 (MQ=255)

                                                      tGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAttt                                   <  2:153971/93‑1 (MQ=255)

                                                       gAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTc                                  >  2:3962549/1‑93 (MQ=255)

                                                        aaGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTcg                                 <  1:3878077/93‑1 (MQ=255)

                                                         aGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTcgc                                <  2:2259167/93‑1 (MQ=255)

                                                              cAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGt                           <  2:4331050/93‑1 (MQ=255)

                                                              aaGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGt                           <  2:373573/92‑1 (MQ=255)

                                                                ggAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGc                         >  1:2068530/1‑93 (MQ=255)

                                                                ggAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGc                         >  1:3405969/1‑93 (MQ=255)

                                                                 gAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCg                        >  2:3640700/1‑93 (MQ=255)

                                                                 gAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCg                        >  1:1619687/1‑93 (MQ=255)

                                                                  aaGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGa                       <  2:370315/93‑1 (MQ=255)

                                                                   agagGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGAc                      <  1:3608436/93‑1 (MQ=255)

                                                                      ggTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGa                   <  1:3235620/93‑1 (MQ=255)

                                                                      ggTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGa                   <  1:606134/93‑1 (MQ=255)

                                                                      ggTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGa                   >  1:1350191/1‑93 (MQ=255)

                                                                       gTGGGGAAAGCCATCTACGCCCGCCGGATGAACAGCATTGAGCAGTCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAt                  <  2:453010/93‑1 (MQ=255)

                                                                        tGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATg                 <  2:3818429/93‑1 (MQ=255)

                                                                        tGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATg                 <  2:4634945/93‑1 (MQ=255)

                                                                          gCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTa               >  1:4412515/1‑93 (MQ=255)

                                                                           cGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAc              <  1:2009103/93‑1 (MQ=255)

                                                                            gAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTAcc             <  2:1803193/93‑1 (MQ=255)

                                                                             aaaGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCt            <  2:1120945/93‑1 (MQ=255)

                                                                             aaaGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCt            <  2:1906684/93‑1 (MQ=255)

                                                                                gCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGcc         >  1:4147257/1‑93 (MQ=255)

                                                                                  cATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCgg       >  1:4668920/1‑92 (MQ=255)

                                                                                  cATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGa       <  1:153398/93‑1 (MQ=255)

                                                                                  cATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGa       >  2:915445/1‑93 (MQ=255)

                                                                                   aTCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAg      <  2:3025803/93‑1 (MQ=255)

                                                                                     cTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGa    <  2:327332/93‑1 (MQ=255)

                                                                                      tACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGaa   >  2:1209651/1‑93 (MQ=255)

                                                                                      tACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGaa   >  1:1638296/1‑93 (MQ=255)

                                                                                       aCGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAAt  <  1:2704879/93‑1 (MQ=255)

ATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAAT  >  NC_000913/3967217‑3967396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACC  >  NC_000913/3967216‑3967400

AATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCC                                                                                             <  SRR4302535.4130516/94‑1 (MQ=60)

 ATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCG                                                                                            >  SRR4302535.2298150/1‑94 (MQ=60)

    ATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCG                                                                                         >  SRR4302535.3503052/1‑94 (MQ=60)

    ATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCG                                                                                         >  SRR4302535.2893011/1‑94 (MQ=60)

     TGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGG                                                                                        <  SRR4302535.3060692/94‑1 (MQ=60)

       AGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGAT                                                                                      <  SRR4302535.2644039/94‑1 (MQ=60)

           GAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAAC                                                                                  <  SRR4302535.1202067/94‑1 (MQ=60)

           GAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGCCGAAAGCCATCTACGGCCGCCGGATGAAC                                                                                  <  SRR4302535.4500451/94‑1 (MQ=60)

              AGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGC                                                                               <  SRR4302535.2845780/94‑1 (MQ=60)

              AGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGC                                                                               <  SRR4302535.127368/94‑1 (MQ=60)

               GGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCA                                                                              >  SRR4302535.1264822/1‑94 (MQ=60)

               GGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCA                                                                              <  SRR4302535.4651893/94‑1 (MQ=60)

                GCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCAT                                                                             <  SRR4302535.409102/94‑1 (MQ=60)

                GCGCATGGGCCGCGCGTACCATCCAGATGAAAGCTCAGGTCAAGCGTCAGGAAGAGGTGGCGAAAGACATCTACGGCCGCCGGATGAACAGCAT                                                                             <  SRR4302535.342355/94‑1 (MQ=60)

                 CGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATT                                                                            <  SRR4302535.971336/94‑1 (MQ=60)

                 CGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATT                                                                            <  SRR4302535.3684456/94‑1 (MQ=60)

                 CGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATT                                                                            <  SRR4302535.3575363/94‑1 (MQ=60)

                  GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           >  SRR4302535.4508911/1‑94 (MQ=60)

                  GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           >  SRR4302535.900705/1‑94 (MQ=60)

                  GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           >  SRR4302535.3015576/1‑94 (MQ=60)

                  GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           >  SRR4302535.4194222/1‑94 (MQ=60)

                  GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           <  SRR4302535.112035/94‑1 (MQ=60)

                  GCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTG                                                                           >  SRR4302535.105819/1‑94 (MQ=60)

                   CATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGA                                                                          >  SRR4302535.1529063/1‑94 (MQ=60)

                   CATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGA                                                                          <  SRR4302535.1873405/94‑1 (MQ=60)

                    ATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAG                                                                         >  SRR4302535.2519481/1‑94 (MQ=60)

                    ATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAG                                                                         <  SRR4302535.1549852/94‑1 (MQ=60)

                     TGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGC                                                                        <  SRR4302535.3346971/94‑1 (MQ=60)

                      GGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCA                                                                       <  SRR4302535.2488469/94‑1 (MQ=60)

                      GGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCA                                                                       <  SRR4302535.1175632/94‑1 (MQ=60)

                      GGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCA                                                                       <  SRR4302535.1184588/94‑1 (MQ=60)

                      GGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGGGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTAAGCA                                                                       >  SRR4302535.1018968/1‑94 (MQ=60)

                       GGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAG                                                                      <  SRR4302535.3348549/94‑1 (MQ=60)

                        GCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGG                                                                     <  SRR4302535.4346495/94‑1 (MQ=60)

                         CGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGC                                                                    <  SRR4302535.2372337/94‑1 (MQ=60)

                         CGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGC                                                                    <  SRR4302535.2345146/94‑1 (MQ=60)

                            CGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCT                                                                 <  SRR4302535.2818028/94‑1 (MQ=60)

                             GCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTG                                                                >  SRR4302535.2087826/1‑94 (MQ=60)

                             GCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTG                                                                <  SRR4302535.2781334/94‑1 (MQ=60)

                             GCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTG                                                                <  SRR4302535.1236839/94‑1 (MQ=60)

                               GTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAA                                                              <  SRR4302535.3603827/94‑1 (MQ=60)

                                TACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAA                                                             >  SRR4302535.2056694/1‑94 (MQ=60)

                                TACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAA                                                             >  SRR4302535.2980196/1‑94 (MQ=60)

                                 ACCATCCAGATGAAAGCTTAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAA                                                            <  SRR4302535.609189/94‑1 (MQ=60)

                                  CCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAT                                                           <  SRR4302535.994392/94‑1 (MQ=60)

                                  CCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAT                                                           <  SRR4302535.3186338/94‑1 (MQ=60)

                                  CCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAAT                                                           >  SRR4302535.1114289/1‑94 (MQ=60)

                                   CATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATT                                                          >  SRR4302535.2385796/1‑94 (MQ=60)

                                   CATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATT                                                          <  SRR4302535.680461/94‑1 (MQ=60)

                                   CATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATT                                                          <  SRR4302535.1454816/94‑1 (MQ=60)

                                    ATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGTCGCTGAAAATTG                                                         >  SRR4302535.4103798/1‑94 (MQ=60)

                                    ATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTG                                                         <  SRR4302535.3438730/94‑1 (MQ=60)

                                    ATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTG                                                         <  SRR4302535.4329353/94‑1 (MQ=60)

                                    ATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTG                                                         <  SRR4302535.95735/94‑1 (MQ=60)

                                     TCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGC                                                        <  SRR4302535.987596/94‑1 (MQ=60)

                                      CCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCT                                                       <  SRR4302535.2540694/94‑1 (MQ=60)

                                      CCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCT                                                       <  SRR4302535.1348407/94‑1 (MQ=60)

                                        AGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGA                                                     >  SRR4302535.482632/1‑94 (MQ=60)

                                        AGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGA                                                     >  SRR4302535.3504853/1‑94 (MQ=60)

                                         GATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCGATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAG                                                    <  SRR4302535.2351569/94‑1 (MQ=60)

                                          ATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGCAAATTGCTGAGC                                                   >  SRR4302535.2351273/1‑94 (MQ=60)

                                          ATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGC                                                   >  SRR4302535.1189437/1‑94 (MQ=60)

                                          ATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGC                                                   >  SRR4302535.1377264/1‑94 (MQ=60)

                                            GAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAG                                                 <  SRR4302535.3578822/94‑1 (MQ=60)

                                             AAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGC                                                <  SRR4302535.2314806/94‑1 (MQ=60)

                                                 CTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAA                                            >  SRR4302535.1206398/1‑94 (MQ=60)

                                                  TCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAAT                                           >  SRR4302535.4589000/1‑94 (MQ=60)

                                                  TCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAAT                                           <  SRR4302535.397680/94‑1 (MQ=60)

                                                    AGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAT                                         >  SRR4302535.4626402/1‑94 (MQ=60)

                                                    AGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAT                                         <  SRR4302535.1058454/94‑1 (MQ=60)

                                                    AGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAT                                         >  SRR4302535.2558992/1‑94 (MQ=60)

                                                    AGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAT                                         >  SRR4302535.2943028/1‑94 (MQ=60)

                                                    AGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATAT                                         <  SRR4302535.2076059/94‑1 (MQ=60)

                                                      GTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTT                                       <  SRR4302535.2201676/94‑1 (MQ=60)

                                                       TGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTC                                      >  SRR4302535.4711018/1‑94 (MQ=60)

                                                       TGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTC                                      <  SRR4302535.3868397/94‑1 (MQ=60)

                                                       TGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTC                                      <  SRR4302535.2081784/94‑1 (MQ=60)

                                                        GAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCG                                     <  SRR4302535.3356568/94‑1 (MQ=60)

                                                        GAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCG                                     <  SRR4302535.1326028/94‑1 (MQ=60)

                                                        GAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCG                                     <  SRR4302535.718574/94‑1 (MQ=60)

                                                         AAGCGTCAGGAAGAGTTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGC                                    <  SRR4302535.4119118/94‑1 (MQ=60)

                                                         AAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGC                                    <  SRR4302535.3904707/94‑1 (MQ=60)

                                                          AGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCG                                   <  SRR4302535.1678599/94‑1 (MQ=60)

                                                          AGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCG                                   <  SRR4302535.355121/94‑1 (MQ=60)

                                                            CGTCAGGAAGAGGTGGCGAAAGCCATCTACGGACCCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCA                                 >  SRR4302535.4469484/1‑94 (MQ=60)

                                                              TCAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGT                               <  SRR4302535.2798002/94‑1 (MQ=60)

                                                               CAGGAAGATGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTG                              <  SRR4302535.797182/94‑1 (MQ=60)

                                                               CAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTG                              >  SRR4302535.3648044/1‑94 (MQ=60)

                                                               CAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTG                              <  SRR4302535.2895917/94‑1 (MQ=60)

                                                               CAGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTG                              <  SRR4302535.1963846/94‑1 (MQ=60)

                                                                AGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGC                             >  SRR4302535.2083441/1‑94 (MQ=60)

                                                                AGGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGC                             >  SRR4302535.3429672/1‑94 (MQ=60)

                                                                 GGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCG                            >  SRR4302535.1631369/1‑94 (MQ=60)

                                                                 GGAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCG                            <  SRR4302535.2045453/94‑1 (MQ=60)

                                                                  GAAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGA                           >  SRR4302535.4062061/1‑94 (MQ=60)

                                                                   AAGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGAC                          >  SRR4302535.371451/1‑94 (MQ=60)

                                                                    AGAGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACA                         <  SRR4302535.3633459/94‑1 (MQ=60)

                                                                      AGGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGA                       >  SRR4302535.1359834/1‑94 (MQ=60)

                                                                       GGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAT                      <  SRR4302535.3258079/94‑1 (MQ=60)

                                                                       GGTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGAT                      <  SRR4302535.610369/94‑1 (MQ=60)

                                                                        GTGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATG                     <  SRR4302535.3171903/94‑1 (MQ=60)

                                                                         TGGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGT                    <  SRR4302535.3292954/94‑1 (MQ=60)

                                                                          GGCGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTA                   >  SRR4302535.4442713/1‑94 (MQ=60)

                                                                            CGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACC                 >  SRR4302535.4688735/1‑94 (MQ=60)

                                                                            CGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACC                 >  SRR4302535.3961675/1‑94 (MQ=60)

                                                                            CGAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACC                 <  SRR4302535.2023571/94‑1 (MQ=60)

                                                                             GAAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCT                <  SRR4302535.1859520/94‑1 (MQ=60)

                                                                              AAAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTC               >  SRR4302535.3001325/1‑94 (MQ=60)

                                                                               AAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGC              >  SRR4302535.1398225/1‑94 (MQ=60)

                                                                               AAGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGC              >  SRR4302535.1647431/1‑94 (MQ=60)

                                                                                AGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCC             >  SRR4302535.4175655/1‑94 (MQ=60)

                                                                                AGCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCC             >  SRR4302535.613880/1‑94 (MQ=60)

                                                                                 GCCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCG            <  SRR4302535.1328564/94‑1 (MQ=60)

                                                                                  CCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGG           >  SRR4302535.4700463/1‑94 (MQ=60)

                                                                                  CCATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGA           >  SRR4302535.1353786/1‑94 (MQ=60)

                                                                                   CATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAG          >  SRR4302535.572334/1‑94 (MQ=60)

                                                                                   CATCTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAG          <  SRR4302535.154475/94‑1 (MQ=60)

                                                                                      CTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGGGCAGTGCGACAGATGTACCTGCGGAGGAA       >  SRR4302535.3233743/1‑94 (MQ=60)

                                                                                      CTACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAA       >  SRR4302535.1650142/1‑94 (MQ=60)

                                                                                       TACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAAT      <  SRR4302535.4198678/94‑1 (MQ=60)

                                                                                        ACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATT     <  SRR4302535.1561967/94‑1 (MQ=60)

                                                                                        ACGGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATT     <  SRR4302535.2724209/94‑1 (MQ=60)

                                                                                          GGCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTAC   <  SRR4302535.2124134/94‑1 (MQ=60)

                                                                                           GCCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACC  <  SRR4302535.3211845/94‑1 (MQ=60)

AATGATGAGCTGAAAGGCGCATGGGCGGCGCGTACCATCCAGATGAAAGCTCAGGTGAAGCGTCAGGAAGAGGTGGCGAAAGCCATCTACGACCGCCGGATGAACAGCATTGAGCAGGCGCTGAAAATTGCTGAGCAGCATAATATTTCGCGCAGTGCGACAGATGTACCTGCCGAGGAATTACC  >  NC_000913/3967216‑3967400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`