Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,978,702 C→T S68F (TCC→TTC)  yifK → putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,978,7020CT99.6% 923.7 / NA 257S68F (TCC→TTC) yifKputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (125/131);  minor base G (0/1);  total (125/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGG  >  NC_000913/3978612‑3978790
                                                                                          |                                                                                        
 aaaaTGGGCGGGACCATCCTTATTGTTGACCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCt                                                                                       <  2:3201727/92‑1 (MQ=255)
  aaaTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTc                                                                                      <  2:3603587/93‑1 (MQ=255)
   aaTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCg                                                                                     >  1:356220/1‑93 (MQ=255)
   aaTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCg                                                                                     >  1:4258521/1‑93 (MQ=255)
    aTGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGa                                                                                    >  1:2862111/1‑93 (MQ=255)
     tGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa                                                                                   <  1:938193/93‑1 (MQ=255)
     tGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa                                                                                   <  1:2705359/93‑1 (MQ=255)
        gccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccg                                                                                >  2:2756410/1‑93 (MQ=255)
        gccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccg                                                                                >  2:4262146/1‑93 (MQ=255)
          cgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggt                                                                              <  2:87333/93‑1 (MQ=255)
             gccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttac                                                                           >  1:1917896/1‑93 (MQ=255)
              ccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttacc                                                                          <  1:2455695/93‑1 (MQ=255)
               cATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccg                                                                         >  1:223910/1‑93 (MQ=255)
                aTCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccgg                                                                        >  1:4459779/1‑93 (MQ=255)
                aTCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccgg                                                                        <  1:1543401/93‑1 (MQ=255)
                 tCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggt                                                                       <  2:2170833/93‑1 (MQ=255)
                 tCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggt                                                                       >  2:1080750/1‑93 (MQ=255)
                   cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                     >  2:4282141/1‑93 (MQ=255)
                   cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                     >  2:3630834/1‑93 (MQ=255)
                    gTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcg                                                                    >  2:2900889/1‑93 (MQ=255)
                     tATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt                                                                   >  1:1835127/1‑93 (MQ=255)
                     tATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt                                                                   >  1:792915/1‑93 (MQ=255)
                     tATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt                                                                   >  1:4201635/1‑93 (MQ=255)
                      aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgtt                                                                  >  1:1652690/1‑93 (MQ=255)
                       ttgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc                                                                 <  1:4475238/93‑1 (MQ=255)
                       ttgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc                                                                 <  1:543606/93‑1 (MQ=255)
                         gttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGc                                                               >  2:1132232/1‑93 (MQ=255)
                         gttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAAACGGTTAACGGTTTGTTCGc                                                               >  2:182006/1‑93 (MQ=255)
                             gCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                           <  1:4283292/93‑1 (MQ=255)
                             gCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                           <  1:1015325/93‑1 (MQ=255)
                               cTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa                                                         <  1:3522067/93‑1 (MQ=255)
                               cTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa                                                         >  1:3979710/1‑93 (MQ=255)
                               cTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa                                                         >  2:3834764/1‑93 (MQ=255)
                                tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt                                                        <  2:1212381/93‑1 (MQ=255)
                                tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt                                                        >  1:4563963/1‑93 (MQ=255)
                                 atatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATg                                                       >  1:2004070/1‑93 (MQ=255)
                                 atatCATCGCCGGGCTGGTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATg                                                       <  2:3858684/93‑1 (MQ=255)
                                  tatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgc                                                      >  2:4544213/1‑93 (MQ=255)
                                   atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcg                                                     >  1:2327271/1‑93 (MQ=255)
                                    tcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc                                                    >  2:3149179/1‑93 (MQ=255)
                                     catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                   <  1:1735469/93‑1 (MQ=255)
                                     catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                   <  2:732611/93‑1 (MQ=255)
                                      atcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAt                                                  >  2:4345252/1‑93 (MQ=255)
                                       tcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATc                                                 <  2:4110315/93‑1 (MQ=255)
                                       tcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATc                                                 >  1:3842079/1‑93 (MQ=255)
                                           cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTta                                             >  1:4602585/1‑93 (MQ=255)
                                           cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTta                                             <  2:528024/93‑1 (MQ=255)
                                           cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTta                                             <  2:2070315/93‑1 (MQ=255)
                                           cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTta                                             >  1:1189847/1‑93 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                           <  1:4325767/93‑1 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                           <  1:4513505/93‑1 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                           <  1:4665356/93‑1 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                           >  1:4137054/1‑93 (MQ=255)
                                                tGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa                                        <  2:1969151/93‑1 (MQ=255)
                                                tGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa                                        >  1:105199/1‑93 (MQ=255)
                                                tGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa                                        <  1:2789978/93‑1 (MQ=255)
                                                tGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa                                        >  1:303676/1‑93 (MQ=255)
                                                tGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCACGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa                                        >  1:2604053/1‑93 (MQ=255)
                                                 gTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAg                                       >  2:1648683/1‑93 (MQ=255)
                                                  ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                      <  2:3262368/93‑1 (MQ=255)
                                                  ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                      >  2:780915/1‑93 (MQ=255)
                                                  ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                      >  2:3330815/1‑93 (MQ=255)
                                                   tcatCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGcc                                     <  1:128191/90‑1 (MQ=255)
                                                   tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGcc                                     >  2:1945787/1‑93 (MQ=255)
                                                     gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg                                   <  1:3618783/93‑1 (MQ=255)
                                                      tCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGt                                  >  2:2220480/1‑93 (MQ=255)
                                                      tCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGt                                  >  1:3956816/1‑93 (MQ=255)
                                                      tCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGCTTATGCGCATCGTTATATGAGCCCGt                                  >  1:2543265/1‑93 (MQ=255)
                                                       cTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGtt                                 <  1:389220/93‑1 (MQ=255)
                                                       cTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGtt                                 <  1:3439470/93‑1 (MQ=255)
                                                        tttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTc                                <  2:37074/93‑1 (MQ=255)
                                                        tttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTc                                >  1:1177071/1‑93 (MQ=255)
                                                         ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTTTATGAGCCCGTTCt                               >  2:2361129/1‑93 (MQ=255)
                                                         ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCt                               <  2:3932953/93‑1 (MQ=255)
                                                         ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCt                               >  1:952097/1‑93 (MQ=255)
                                                         ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCt                               <  2:1024791/93‑1 (MQ=255)
                                                          tttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCtt                              <  2:3303062/93‑1 (MQ=255)
                                                          tttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCtt                              <  2:3110155/93‑1 (MQ=255)
                                                           ttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt                             >  1:4083201/1‑93 (MQ=255)
                                                            tcatcaTGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTg                            <  1:1469358/93‑1 (MQ=255)
                                                              atcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGc                          >  2:2524567/1‑93 (MQ=255)
                                                               tcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCt                         >  1:2991105/1‑93 (MQ=255)
                                                                 atGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAt                       >  1:334376/1‑93 (MQ=255)
                                                                 atGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAt                       <  2:3994737/93‑1 (MQ=255)
                                                                  tGCGTTCAATGGGCGAAATGTTGTGCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATAAGCCCGTTCTGTGGCTAtc                      <  2:4585811/93‑1 (MQ=255)
                                                                   gCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtct                     <  2:269224/93‑1 (MQ=255)
                                                                   ccGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtct                     <  2:1147373/92‑1 (MQ=255)
                                                                     gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa                   >  2:617799/1‑93 (MQ=255)
                                                                       tCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAcc                 >  2:1382528/1‑93 (MQ=255)
                                                                        cAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCg                <  1:3732465/93‑1 (MQ=255)
                                                                         aaTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTGGTTCGCCGTTTATGCGCATCGTTATATAAGCCCGTTTTTTGGCTACTCCACCGc               >  2:131759/1‑93 (MQ=255)
                                                                         aaTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGc               >  2:4182267/1‑93 (MQ=255)
                                                                           tGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCt             <  2:4436529/93‑1 (MQ=255)
                                                                            gggCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTg            >  1:1707142/1‑93 (MQ=255)
                                                                            gggCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTg            >  1:1220962/1‑93 (MQ=255)
                                                                               cGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTc         <  2:686993/93‑1 (MQ=255)
                                                                                   aTGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAc     <  2:2909253/93‑1 (MQ=255)
                                                                                    tgttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACt    <  2:1476931/93‑1 (MQ=255)
                                                                                      ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg  >  2:1836181/1‑93 (MQ=255)
                                                                                      ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg  >  2:1752890/1‑93 (MQ=255)
                                                                                          |                                                                                        
TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGG  >  NC_000913/3978612‑3978790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  >  NC_000913/3978609‑3978795
                                                                                             |                                                                                             
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTT                                                                                               >  SRR4302535.623355/1‑94 (MQ=60)
 CCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTC                                                                                              >  SRR4302535.3111318/1‑94 (MQ=60)
   TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCT                                                                                            <  SRR4302535.3573187/94‑1 (MQ=60)
   TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCT                                                                                            >  SRR4302535.1225555/1‑94 (MQ=60)
   TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCT                                                                                            <  SRR4302535.4538884/94‑1 (MQ=60)
    TAAATGGGCCCGTCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTC                                                                                           <  SRR4302535.3917224/94‑1 (MQ=60)
    GAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTC                                                                                           <  SRR4302535.3766153/94‑1 (MQ=60)
     AAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCG                                                                                          >  SRR4302535.358681/1‑94 (MQ=60)
     AAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCG                                                                                          <  SRR4302535.4589558/94‑1 (MQ=60)
     AAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCG                                                                                          >  SRR4302535.4287691/1‑94 (MQ=60)
      AATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGA                                                                                         >  SRR4302535.2882386/1‑94 (MQ=60)
        TGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAAC                                                                                       <  SRR4302535.944838/94‑1 (MQ=60)
        TGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAAC                                                                                       <  SRR4302535.2724691/94‑1 (MQ=60)
         GGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACC                                                                                      <  SRR4302535.2512080/94‑1 (MQ=60)
              GGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTA                                                                                 >  SRR4302535.3010466/1‑94 (MQ=60)
               GGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTAC                                                                                >  SRR4302535.1931716/1‑94 (MQ=60)
                GCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACC                                                                               >  SRR4302535.3214078/1‑94 (MQ=60)
                 CCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCG                                                                              <  SRR4302535.2473400/94‑1 (MQ=60)
                 CCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCG                                                                              >  SRR4302535.893890/1‑94 (MQ=60)
                 CCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCG                                                                              >  SRR4302535.225450/1‑94 (MQ=60)
                  CATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGG                                                                             >  SRR4302535.4490367/1‑94 (MQ=60)
                   ATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGT                                                                            <  SRR4302535.1554523/94‑1 (MQ=60)
                   ATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGT                                                                            >  SRR4302535.4331354/1‑94 (MQ=60)
                       GTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGT                                                                        >  SRR4302535.798488/1‑94 (MQ=60)
                       GTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGT                                                                        >  SRR4302535.3871153/1‑94 (MQ=60)
                       GTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGT                                                                        >  SRR4302535.4230383/1‑94 (MQ=60)
                       GTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGT                                                                        >  SRR4302535.1848367/1‑94 (MQ=60)
                        TATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTT                                                                       >  SRR4302535.1664650/1‑94 (MQ=60)
                          TTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCG                                                                     <  SRR4302535.2656966/94‑1 (MQ=60)
                          TTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCG                                                                     <  SRR4302535.547406/94‑1 (MQ=60)
                          TTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCG                                                                     <  SRR4302535.4505944/94‑1 (MQ=60)
                          TTGTTGGCCTATATCATCGCCGGCCTGTTCGTTTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGACCCGGTTCCCGGTTCGTTCG                                                                     <  SRR4302535.4505945/94‑1 (MQ=60)
                            TTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCC                                                                   <  SRR4302535.974853/94‑1 (MQ=60)
                            GTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCC                                                                   >  SRR4302535.973186/1‑94 (MQ=60)
                               GGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTT                                                                >  SRR4302535.1515639/1‑94 (MQ=60)
                                GCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTT                                                               <  SRR4302535.1022544/94‑1 (MQ=60)
                                GCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTT                                                               <  SRR4302535.4312596/94‑1 (MQ=60)
                                 CCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTA                                                              >  SRR4302535.4006900/1‑94 (MQ=60)
                                  CTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAT                                                             <  SRR4302535.3546530/94‑1 (MQ=60)
                                  CTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAT                                                             >  SRR4302535.4595107/1‑94 (MQ=60)
                                   TATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATG                                                            <  SRR4302535.4112505/94‑1 (MQ=60)
                                   TATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATG                                                            >  SRR4302535.2018512/1‑94 (MQ=60)
                                    ATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGC                                                           <  SRR4302535.3325800/94‑1 (MQ=60)
                                    ATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGC                                                           >  SRR4302535.4198140/1‑94 (MQ=60)
                                     TATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCG                                                          >  SRR4302535.2343923/1‑94 (MQ=60)
                                     TATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCG                                                          >  SRR4302535.4488275/1‑94 (MQ=60)
                                      ATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGC                                                         >  SRR4302535.2154419/1‑94 (MQ=60)
                                      ATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGC                                                         <  SRR4302535.2879109/94‑1 (MQ=60)
                                      ATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGC                                                         >  SRR4302535.3765421/1‑94 (MQ=60)
                                        CATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAT                                                       >  SRR4302535.1178943/1‑94 (MQ=60)
                                        CATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAT                                                       <  SRR4302535.1748015/94‑1 (MQ=60)
                                         ATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATC                                                      >  SRR4302535.1451285/1‑94 (MQ=60)
                                         ATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATC                                                      >  SRR4302535.3868548/1‑94 (MQ=60)
                                            GCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTT                                                   <  SRR4302535.1524173/94‑1 (MQ=60)
                                            GCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTT                                                   <  SRR4302535.4633147/94‑1 (MQ=60)
                                             CCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTA                                                  >  SRR4302535.1198367/1‑94 (MQ=60)
                                             CCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTA                                                  >  SRR4302535.4633884/1‑94 (MQ=60)
                                              CGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTAT                                                 <  SRR4302535.4320706/94‑1 (MQ=60)
                                               GGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATA                                                >  SRR4302535.1732748/1‑94 (MQ=60)
                                               GGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATA                                                >  SRR4302535.4165384/1‑94 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302535.305267/94‑1 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302535.4544456/94‑1 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               >  SRR4302535.2022520/1‑94 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302535.1611757/94‑1 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302535.4355348/94‑1 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302535.4696896/94‑1 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302535.466405/94‑1 (MQ=60)
                                                 GCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATG                                              >  SRR4302535.3998092/1‑94 (MQ=60)
                                                  CTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGA                                             >  SRR4302535.1193077/1‑94 (MQ=60)
                                                  CTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGA                                             >  SRR4302535.105945/1‑94 (MQ=60)
                                                  CTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGA                                             >  SRR4302535.1986446/1‑94 (MQ=60)
                                                  CTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGA                                             >  SRR4302535.305784/1‑94 (MQ=60)
                                                  CTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGA                                             >  SRR4302535.3403108/1‑94 (MQ=60)
                                                  CTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCACGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGA                                             >  SRR4302535.2622747/1‑94 (MQ=60)
                                                   TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGACATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAG                                            <  SRR4302535.2599310/94‑1 (MQ=60)
                                                   TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAG                                            >  SRR4302535.1820740/1‑94 (MQ=60)
                                                   TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAG                                            >  SRR4302535.95455/1‑94 (MQ=60)
                                                   TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAG                                            <  SRR4302535.2809835/94‑1 (MQ=60)
                                                   TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGGTCGCCGTTTATGCGCATCGCTATATGAG                                            >  SRR4302535.1865640/1‑94 (MQ=60)
                                                    GTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGCTACCGGGTCGCTCGCCGTTTATGCGCATCGTTATATGAGC                                           >  SRR4302535.3924948/1‑94 (MQ=60)
                                                      TCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCC                                         <  SRR4302535.4004615/94‑1 (MQ=60)
                                                      TCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCC                                         <  SRR4302535.2466689/94‑1 (MQ=60)
                                                      TCATCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCC                                         <  SRR4302535.129095/94‑1 (MQ=60)
                                                       CGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCG                                        >  SRR4302535.4305144/1‑94 (MQ=60)
                                                       CGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCG                                        <  SRR4302535.2926820/94‑1 (MQ=60)
                                                       CGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCG                                        >  SRR4302535.3427089/1‑94 (MQ=60)
                                                        GTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGT                                       <  SRR4302535.3643870/94‑1 (MQ=60)
                                                        GTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGT                                       >  SRR4302535.3983831/1‑94 (MQ=60)
                                                        GTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGCTTATGCGCATCGTTATATGAGCCCGT                                       >  SRR4302535.2561578/1‑94 (MQ=60)
                                                         TCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTT                                      <  SRR4302535.2363782/94‑1 (MQ=60)
                                                         TCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTT                                      <  SRR4302535.518088/94‑1 (MQ=60)
                                                         TCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTT                                      >  SRR4302535.2749679/1‑94 (MQ=60)
                                                          CTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTC                                     <  SRR4302535.842905/94‑1 (MQ=60)
                                                          CTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTC                                     <  SRR4302535.3463389/94‑1 (MQ=60)
                                                          CTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTC                                     >  SRR4302535.1185472/1‑94 (MQ=60)
                                                          CTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTC                                     <  SRR4302535.391927/94‑1 (MQ=60)
                                                          CTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTC                                     >  SRR4302535.3635456/1‑94 (MQ=60)
                                                           TTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCGTCGTTATATGAGCCCGTTCT                                    <  SRR4302535.4500303/94‑1 (MQ=60)
                                                           TTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCT                                    >  SRR4302535.958846/1‑94 (MQ=60)
                                                           TTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCT                                    <  SRR4302535.2655528/94‑1 (MQ=60)
                                                             TTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTT                                  >  SRR4302535.4111126/1‑94 (MQ=60)
                                                              TTCATCATTCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTG                                 <  SRR4302535.4370081/94‑1 (MQ=60)
                                                              TTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTG                                 <  SRR4302535.2319677/94‑1 (MQ=60)
                                                              TTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTG                                 >  SRR4302535.3550783/1‑94 (MQ=60)
                                                               TCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGG                                <  SRR4302535.4256711/94‑1 (MQ=60)
                                                               TCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGG                                <  SRR4302535.1479883/94‑1 (MQ=60)
                                                                CATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGC                               <  SRR4302535.4600608/94‑1 (MQ=60)
                                                                CATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGC                               >  SRR4302535.4649019/1‑94 (MQ=60)
                                                                CATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGC                               >  SRR4302535.1252306/1‑94 (MQ=60)
                                                                CATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGC                               >  SRR4302535.4283769/1‑94 (MQ=60)
                                                                 ATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCT                              >  SRR4302535.3012078/1‑94 (MQ=60)
                                                                 ATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCT                              >  SRR4302535.658535/1‑94 (MQ=60)
                                                                 ATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCT                              <  SRR4302535.1039346/94‑1 (MQ=60)
                                                                   CATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAT                            >  SRR4302535.1665532/1‑94 (MQ=60)
                                                                   CATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAT                            >  SRR4302535.336689/1‑94 (MQ=60)
                                                                   CATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAT                            >  SRR4302535.2755067/1‑94 (MQ=60)
                                                                    ATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATC                           <  SRR4302535.638504/94‑1 (MQ=60)
                                                                     TGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCT                          <  SRR4302535.2496241/94‑1 (MQ=60)
                                                                        GTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAC                       >  SRR4302535.195771/1‑94 (MQ=60)
                                                                         TTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACC                      <  SRR4302535.1226919/94‑1 (MQ=60)
                                                                          TCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCG                     <  SRR4302535.3593265/94‑1 (MQ=60)
                                                                           CAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGC                    >  SRR4302535.1746887/1‑94 (MQ=60)
                                                                           CAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGC                    <  SRR4302535.3758307/94‑1 (MQ=60)
                                                                            AATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCC                   <  SRR4302535.4095926/94‑1 (MQ=60)
                                                                              TGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTG                 >  SRR4302535.1719485/1‑94 (MQ=60)
                                                                              TGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTG                 >  SRR4302535.1229712/1‑94 (MQ=60)
                                                                               GGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGG                <  SRR4302535.3523047/94‑1 (MQ=60)
                                                                               GGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGG                <  SRR4302535.1275343/94‑1 (MQ=60)
                                                                                GGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGT               <  SRR4302535.3685/94‑1 (MQ=60)
                                                                                   GAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTT            <  SRR4302535.1622583/94‑1 (MQ=60)
                                                                                   GAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGGTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTT            >  SRR4302535.4216823/1‑94 (MQ=60)
                                                                                    AAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTA           <  SRR4302535.1785034/94‑1 (MQ=60)
                                                                                     AATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAC          <  SRR4302535.1154562/94‑1 (MQ=60)
                                                                                      ATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACT         <  SRR4302535.4015756/94‑1 (MQ=60)
                                                                                           GTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTT    <  SRR4302535.4449161/94‑1 (MQ=60)
                                                                                             TCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  >  SRR4302535.675417/1‑94 (MQ=60)
                                                                                             TCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  <  SRR4302535.2668790/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  >  NC_000913/3978609‑3978795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑