Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,001,421 2 bp→GC coding (381‑382/903 nt) rarD ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,001,4210CG99.5% 569.5 / NA 182G128R (GGA→CGA) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (106/75);  minor base T (0/1);  total (106/76)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,001,4220GC100.0% 575.6 / NA 183D127E (GAC→GAG) rarDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (106/77);  total (106/77)

Only 100 of 191 total aligned reads displayed.

TAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTT  >  NC_000913/4001334‑4001508
                                                                                       ||                                                                                      
taataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAAtcgctcgc                                                                                    >  2:2339862/1‑93 (MQ=255)
taataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAAtcgctcgc                                                                                    >  1:519976/1‑93 (MQ=255)
 aataGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGcc                                                                                   >  1:4659612/1‑93 (MQ=255)
   taGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGa                                                                                 >  2:578776/1‑93 (MQ=255)
    aGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAg                                                                                <  1:2095467/93‑1 (MQ=255)
     ggTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAgg                                                                               >  2:4093649/1‑93 (MQ=255)
      gTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGa                                                                              <  1:2322844/93‑1 (MQ=255)
       tAGCGAACCAAAAGTTCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaa                                                                             >  1:1573193/1‑93 (MQ=255)
         gCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaaaa                                                                           <  1:132982/93‑1 (MQ=255)
         gCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaaaa                                                                           <  1:3243318/93‑1 (MQ=255)
         gCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGaaaa                                                                           >  2:1513884/1‑93 (MQ=255)
             aCCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAatcat                                                                       >  1:505936/1‑93 (MQ=255)
               cAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATcc                                                                     <  2:827229/93‑1 (MQ=255)
               cAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATcc                                                                     >  1:3075860/1‑93 (MQ=255)
                aaaaGTCCACAGCTGGACTAAGACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATccc                                                                    >  2:4252858/1‑93 (MQ=255)
                 aaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATcccc                                                                   >  1:2119851/1‑93 (MQ=255)
                 aaaGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATcccc                                                                   <  1:3520271/93‑1 (MQ=255)
                  aaGTCCACAGCTGGACTAACACGCCCCATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCa                                                                  <  2:175784/93‑1 (MQ=255)
                   aGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAg                                                                 >  2:1258919/1‑93 (MQ=255)
                        ccaGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACaa                                                            <  1:1966864/92‑1 (MQ=255)
                        ccaGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACaa                                                            <  1:1646354/92‑1 (MQ=255)
                        acaGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACaa                                                            <  2:4113980/93‑1 (MQ=255)
                        acaGCGGGCCTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACaa                                                            <  2:853753/93‑1 (MQ=255)
                          aGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATg                                                          >  1:4324576/1‑93 (MQ=255)
                          aGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATg                                                          <  1:3053731/93‑1 (MQ=255)
                           gCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGt                                                         >  2:1468583/1‑93 (MQ=255)
                           gCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGt                                                         >  1:931457/1‑93 (MQ=255)
                           gCTGGACTAACACGCCACATATCACCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGt                                                         <  2:3171592/93‑1 (MQ=255)
                            cTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGtt                                                        >  2:3982109/1‑93 (MQ=255)
                             tGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTc                                                       <  2:461486/93‑1 (MQ=255)
                             gggACTACCACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTc                                                       <  2:127260/92‑1 (MQ=255)
                              ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCa                                                      >  1:3554998/1‑93 (MQ=255)
                              ggACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCa                                                      <  1:1512462/93‑1 (MQ=255)
                               gACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAc                                                     >  1:2048300/1‑93 (MQ=255)
                                aCTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAcc                                                    <  1:625667/93‑1 (MQ=255)
                                aCTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAcc                                                    <  1:1168440/93‑1 (MQ=255)
                                aCTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAcc                                                    >  1:1000403/1‑93 (MQ=255)
                                 cTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCa                                                   <  1:671113/93‑1 (MQ=255)
                                  tAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAg                                                  >  1:1198559/1‑93 (MQ=255)
                                  tAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAg                                                  >  1:2644387/1‑93 (MQ=255)
                                    acacGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCg                                                >  2:2180253/1‑93 (MQ=255)
                                     cacGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCgg                                               >  2:1586075/1‑93 (MQ=255)
                                      acGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCggg                                              >  2:2274954/1‑93 (MQ=255)
                                        gCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGtt                                            >  2:4062058/1‑93 (MQ=255)
                                        cccACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGtt                                            <  1:2293696/92‑1 (MQ=255)
                                         ccACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTta                                           <  2:2222097/93‑1 (MQ=255)
                                          cacaTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaa                                          >  1:784353/1‑93 (MQ=255)
                                           acaTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaat                                         <  2:3181962/93‑1 (MQ=255)
                                           acaTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaat                                         >  1:4171562/1‑93 (MQ=255)
                                            caTATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaata                                        <  2:1384343/93‑1 (MQ=255)
                                             atctCGCCAGAATCACCGCCATCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaataa                                       <  1:2369310/90‑1 (MQ=255)
                                             atatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTtaataa                                       <  2:3234112/93‑1 (MQ=255)
                                              tatCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCTCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      <  1:2646123/93‑1 (MQ=255)
                                              tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      >  2:3933848/1‑93 (MQ=255)
                                              tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      <  2:1966666/93‑1 (MQ=255)
                                              tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      >  1:419976/1‑93 (MQ=255)
                                              tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      >  1:4092377/1‑93 (MQ=255)
                                              tatCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATaaa                                      >  1:4066016/1‑93 (MQ=255)
                                               atCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAg                                     <  2:3664580/93‑1 (MQ=255)
                                                tCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGt                                    <  2:332896/93‑1 (MQ=255)
                                                tCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAga                                    >  1:738739/1‑92 (MQ=255)
                                                  cccAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTaa                                  <  1:2897748/92‑1 (MQ=255)
                                                    cAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAcc                                >  2:3492290/1‑93 (MQ=255)
                                                    cAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAcc                                >  1:8678/1‑93 (MQ=255)
                                                     aGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCa                               >  1:1791536/1‑93 (MQ=255)
                                                     aGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCa                               >  2:380968/1‑93 (MQ=255)
                                                     aGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCa                               >  2:2930397/1‑93 (MQ=255)
                                                      gAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCaa                              <  2:3578896/93‑1 (MQ=255)
                                                       aaTCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAg                             >  1:2844233/1‑93 (MQ=255)
                                                        aTCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAgg                            >  2:168478/1‑93 (MQ=255)
                                                        aTCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGATCACCAGCGGGTTAATAAAGTAACCAAgg                            >  2:2825953/1‑93 (MQ=255)
                                                          cACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCt                          <  2:4342808/93‑1 (MQ=255)
                                                           aCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTc                         <  1:1303163/93‑1 (MQ=255)
                                                            ccGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcg                        <  1:3580565/93‑2 (MQ=255)
                                                            ccGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcg                        >  1:2628943/1‑92 (MQ=255)
                                                              gccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgct                      >  1:632185/1‑90 (MQ=255)
                                                              cccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgct                      <  1:3759036/92‑4 (MQ=255)
                                                               ccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgctt                     >  1:565115/1‑89 (MQ=255)
                                                               ccagccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTcgctt                     <  1:999580/93‑5 (MQ=255)
                                                                 agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                   >  1:2772157/1‑93 (MQ=255)
                                                                 agccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTcc                   >  2:1427013/1‑93 (MQ=255)
                                                                  gccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCa                  >  2:2378696/1‑93 (MQ=255)
                                                                   ccaTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAg                 <  2:1925703/93‑1 (MQ=255)
                                                                     aTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCa               >  2:758213/1‑93 (MQ=255)
                                                                     aTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCa               >  2:2143136/1‑93 (MQ=255)
                                                                     aTTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCa               >  1:3337311/1‑93 (MQ=255)
                                                                      ttGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCat              >  2:1740024/1‑93 (MQ=255)
                                                                      ttGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCat              >  2:246229/1‑93 (MQ=255)
                                                                      ttGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCat              >  1:4584640/1‑93 (MQ=255)
                                                                      ttGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCat              >  2:1799912/1‑93 (MQ=255)
                                                                       tGCGTCCGGCGGAATCGCTCGCCGAGGAAAGTCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCata             <  2:917208/93‑1 (MQ=255)
                                                                        gCATCCGGCGTAATCGCTCGCCGAGGAAAATCAGCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCatat            <  2:694180/93‑1 (MQ=255)
                                                                        gCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCatat            <  1:2982923/93‑1 (MQ=255)
                                                                          aTCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATgg          >  2:2979543/1‑93 (MQ=255)
                                                                           tCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGt         >  1:40407/1‑93 (MQ=255)
                                                                           tCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGt         <  2:2153716/93‑1 (MQ=255)
                                                                           tCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGt         >  1:3675335/1‑93 (MQ=255)
                                                                              ggcggAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGAt      <  1:3398915/93‑1 (MQ=255)
                                                                                  gAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGtt  <  2:4671874/93‑1 (MQ=255)
                                                                                  gAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGtt  <  2:2045596/93‑1 (MQ=255)
                                                                                       ||                                                                                      
TAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTT  >  NC_000913/4001334‑4001508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTCA  >  NC_000913/4001328‑4001510
                                                                                             |                                                                                         
GCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCG                                                                                           <  SRR4302535.251316/94‑1 (MQ=60)
     ATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGC                                                                                      >  SRR4302535.523612/1‑94 (MQ=60)
     ATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGC                                                                                      >  SRR4302535.3953158/1‑94 (MQ=60)
     ATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGC                                                                                      >  SRR4302535.3405098/1‑94 (MQ=60)
      TAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCC                                                                                     >  SRR4302535.4691129/1‑94 (MQ=60)
       AATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCG                                                                                    >  SRR4302535.558063/1‑94 (MQ=60)
       AAGAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCG                                                                                    <  SRR4302535.2315859/94‑1 (MQ=60)
        ATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGA                                                                                   >  SRR4302535.523305/1‑94 (MQ=60)
        ATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGA                                                                                   >  SRR4302535.201033/1‑94 (MQ=60)
          AGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGG                                                                                 <  SRR4302535.2110581/94‑1 (MQ=60)
            GTAGCGAACCAAAAGTTCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAA                                                                               >  SRR4302535.1584550/1‑94 (MQ=60)
            GTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAA                                                                               >  SRR4302535.4684758/1‑94 (MQ=60)
            GTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAA                                                                               <  SRR4302535.2339466/94‑1 (MQ=60)
             TAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAA                                                                              >  SRR4302535.940995/1‑94 (MQ=60)
               GCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAAT                                                                            <  SRR4302535.3265848/94‑1 (MQ=60)
               GCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAAT                                                                            <  SRR4302535.133915/94‑1 (MQ=60)
                 GAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCA                                                                          <  SRR4302535.4238396/94‑1 (MQ=60)
                 GAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCA                                                                          <  SRR4302535.758973/94‑1 (MQ=60)
                  AACCAAAAGTCCGCGGCAGGACTACCACGCCACATATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT                                                                         <  SRR4302535.343819/94‑1 (MQ=60)
                  AACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT                                                                         >  SRR4302535.158959/1‑94 (MQ=60)
                  AACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT                                                                         >  SRR4302535.509472/1‑94 (MQ=60)
                  AACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT                                                                         >  SRR4302535.4207931/1‑94 (MQ=60)
                  AACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT                                                                         >  SRR4302535.824368/1‑94 (MQ=60)
                  AACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAT                                                                         >  SRR4302535.2679500/1‑94 (MQ=60)
                  AACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCAC                                                                         >  SRR4302535.684386/1‑94 (MQ=60)
                    CCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCC                                                                       <  SRR4302535.2160495/94‑1 (MQ=60)
                    CCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCC                                                                       >  SRR4302535.3097321/1‑94 (MQ=60)
                     CAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCC                                                                      >  SRR4302535.370082/1‑94 (MQ=60)
                      AAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGCGGAAAATCATCCCC                                                                     >  SRR4302535.1206188/1‑94 (MQ=60)
                      AAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCC                                                                     >  SRR4302535.2135140/1‑94 (MQ=60)
                       AAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCA                                                                    <  SRR4302535.3544722/94‑1 (MQ=60)
                          GTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCTAGCA                                                                 >  SRR4302535.2968957/1‑94 (MQ=60)
                              CCAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAAT                                                             <  SRR4302535.1658252/94‑1 (MQ=60)
                              CCAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAAT                                                             <  SRR4302535.1981041/94‑1 (MQ=60)
                              ACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAAT                                                             <  SRR4302535.2829462/94‑1 (MQ=60)
                              ACAGCGGGACTAACACGCCACATATCGCCAGAATCTCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAAT                                                             <  SRR4302535.2889049/94‑1 (MQ=60)
                               CAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATG                                                            >  SRR4302535.4354155/1‑94 (MQ=60)
                                AGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGT                                                           <  SRR4302535.3075111/94‑1 (MQ=60)
                                AGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGT                                                           >  SRR4302535.938046/1‑94 (MQ=60)
                                  CTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTC                                                         >  SRR4302535.1293538/1‑94 (MQ=60)
                                  CTGGACGAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTC                                                         <  SRR4302535.1781282/94‑1 (MQ=60)
                                   TGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCA                                                        >  SRR4302535.3579657/1‑94 (MQ=60)
                                    GGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAC                                                       >  SRR4302535.2063049/1‑94 (MQ=60)
                                    GGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCAC                                                       <  SRR4302535.1523357/94‑1 (MQ=60)
                                     GACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACC                                                      >  SRR4302535.1007514/1‑94 (MQ=60)
                                      ACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCA                                                     <  SRR4302535.630018/94‑1 (MQ=60)
                                      ACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCA                                                     <  SRR4302535.1176780/94‑1 (MQ=60)
                                       CTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAG                                                    >  SRR4302535.2663327/1‑94 (MQ=60)
                                       CTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAG                                                    >  SRR4302535.2663326/1‑94 (MQ=60)
                                       CTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAG                                                    >  SRR4302535.1207145/1‑94 (MQ=60)
                                       CTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAG                                                    <  SRR4302535.675799/94‑1 (MQ=60)
                                              GCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTA                                             >  SRR4302535.3082041/1‑94 (MQ=60)
                                              GCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTA                                             <  SRR4302535.1885272/94‑1 (MQ=60)
                                              CCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTA                                             <  SRR4302535.2310103/94‑1 (MQ=60)
                                               CCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAA                                            <  SRR4302535.4489989/94‑1 (MQ=60)
                                               CCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAA                                            >  SRR4302535.2068076/1‑94 (MQ=60)
                                               CCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAA                                            >  SRR4302535.789857/1‑94 (MQ=60)
                                                CACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAAT                                           >  SRR4302535.4200121/1‑94 (MQ=60)
                                                 ACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGACAATCATACCCAGCACAATGTTCACCAGCGGGTTAATA                                          >  SRR4302535.492030/1‑94 (MQ=60)
                                                   ATCTCGCCAGAATCACCGCCATCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAA                                        <  SRR4302535.2386321/94‑1 (MQ=60)
                                                   ATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAA                                        >  SRR4302535.4120382/1‑94 (MQ=60)
                                                   ATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAA                                        >  SRR4302535.1424003/1‑94 (MQ=60)
                                                   ATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAA                                        >  SRR4302535.422912/1‑94 (MQ=60)
                                                   ATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAA                                        >  SRR4302535.4093841/1‑94 (MQ=60)
                                                    TATCGCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCTCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAG                                       <  SRR4302535.2665074/94‑1 (MQ=60)
                                                     ATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGA                                      >  SRR4302535.743934/1‑94 (MQ=60)
                                                      TCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTA                                     <  SRR4302535.4196459/94‑1 (MQ=60)
                                                        CCCAGAATCCCCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAAC                                   <  SRR4302535.2918240/94‑1 (MQ=60)
                                                         CCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACC                                  >  SRR4302535.8749/1‑94 (MQ=60)
                                                          CAGAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCA                                 >  SRR4302535.1804489/1‑94 (MQ=60)
                                                            GAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAG                               >  SRR4302535.2864399/1‑94 (MQ=60)
                                                            GAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAG                               >  SRR4302535.673236/1‑94 (MQ=60)
                                                            GAATCACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAG                               <  SRR4302535.4097008/94‑1 (MQ=60)
                                                            GAATCACCGCCAGCCATTGCATCCAGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTCCACCAGCGGGTTAATAAAGTAACCAAG                               <  SRR4302535.2625395/94‑1 (MQ=60)
                                                                 ACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCG                          >  SRR4302535.2647782/1‑94 (MQ=60)
                                                                 ACCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCG                          <  SRR4302535.1312467/94‑1 (MQ=60)
                                                                  CCGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgc                         <  SRR4302535.3605380/94‑3 (MQ=60)
                                                                   CGCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgct                        >  SRR4302535.636591/1‑91 (MQ=60)
                                                                    GCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgctt                       >  SRR4302535.569073/1‑90 (MQ=60)
                                                                    CCCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgctt                       <  SRR4302535.3785023/94‑5 (MQ=60)
                                                                     CCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgcttc                      <  SRR4302535.1006683/94‑6 (MQ=60)
                                                                     CCAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgcttc                      >  SRR4302535.3958151/1‑89 (MQ=60)
                                                                      CAGCCATTGCATCCGGCGGAATCGCTCTCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCC                     >  SRR4302535.1558339/1‑94 (MQ=60)
                                                                      CAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCC                     <  SRR4302535.1119374/94‑1 (MQ=60)
                                                                      CAGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCC                     >  SRR4302535.2791897/1‑94 (MQ=60)
                                                                       AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCgcttccg                    >  SRR4302535.909026/1‑87 (MQ=60)
                                                                       AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                    >  SRR4302535.3344206/1‑94 (MQ=60)
                                                                       AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                    <  SRR4302535.1965485/94‑1 (MQ=60)
                                                                       AGCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCA                    >  SRR4302535.3690309/1‑94 (MQ=60)
                                                                        GCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAG                   <  SRR4302535.1612704/94‑1 (MQ=60)
                                                                        GCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAG                   <  SRR4302535.4572892/94‑1 (MQ=60)
                                                                        GCCATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAG                   >  SRR4302535.1327464/1‑94 (MQ=60)
                                                                          CATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCA                 >  SRR4302535.3360556/1‑94 (MQ=60)
                                                                           ATTGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCAT                >  SRR4302535.4615859/1‑94 (MQ=60)
                                                                             TGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATAT              <  SRR4302535.356261/94‑1 (MQ=60)
                                                                             TGCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATAT              <  SRR4302535.3192885/94‑1 (MQ=60)
                                                                              GCATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATG             <  SRR4302535.3003845/94‑1 (MQ=60)
                                                                                ATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGT           >  SRR4302535.62574/1‑94 (MQ=60)
                                                                                ATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGT           >  SRR4302535.40714/1‑94 (MQ=60)
                                                                                ATCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGT           >  SRR4302535.3700781/1‑94 (MQ=60)
                                                                                 TCCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTG          >  SRR4302535.2367962/1‑94 (MQ=60)
                                                                                  CCGGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGA         <  SRR4302535.3586693/94‑1 (MQ=60)
                                                                                    GGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATT       <  SRR4302535.3422565/94‑1 (MQ=60)
                                                                                    GGCGGAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATT       >  SRR4302535.2648046/1‑94 (MQ=60)
                                                                                        GAATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTC   >  SRR4302535.1073691/1‑94 (MQ=60)
                                                                                         AATCGCTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCGCTTCCAGCATATGGTGATTGTTCA  <  SRR4302535.4164965/94‑1 (MQ=60)
                                                                                             |                                                                                         
GCGCGATAATAGGTAGCGAACCAAAAGTCCACAGCTGGACTAACACGCCACATATCGCCAGAATCACCGCCAGCCATTGCATCCGGCGGAATCCGTCGCCGAGGAAAATCATCCCCAGCACAATGTTCACCAGCGGGTTAATAAAGTAACCAAGGCTCCGTTCCAGCATATGGTGATTGTTCA  >  NC_000913/4001328‑4001510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑