Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,004,979 +G coding (1511/1833 nt) recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9791.G99.0% 617.6 / ‑1.2 199coding (1511/1833 nt)recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (101/96);  total (102/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

Only 100 of 218 total aligned reads displayed.

GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CG‑CG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004888‑4005060
                                                                                            |                                                                                      
gTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtg                                                                                        >  2:923177/1‑92 (MQ=255)
gTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtg                                                                                        <  2:3406353/93‑2 (MQ=255)
gTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtg                                                                                        <  2:4352251/93‑2 (MQ=255)
 tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                       >  1:3366189/1‑91 (MQ=255)
   aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                     >  1:3329809/1‑89 (MQ=255)
   aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                     <  1:3097872/93‑5 (MQ=255)
      cGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑Cg‑‑gc                                                                               <  2:2127759/93‑2 (MQ=255)
       gCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑Cg‑‑gcg                                                                              >  1:4002730/1‑91 (MQ=255)
        ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑Cg‑‑gcga                                                                             <  1:4656267/93‑4 (MQ=255)
        ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑Cg‑‑gcga                                                                             >  2:311149/1‑90 (MQ=255)
        ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑Cg‑‑gcga                                                                             >  2:4103545/1‑90 (MQ=255)
         cAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑Cg‑‑gcgaa                                                                            >  1:985558/1‑89 (MQ=255)
                ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCtt                                                                     >  1:2153598/1‑93 (MQ=255)
                 tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCttt                                                                    <  1:1831836/93‑1 (MQ=255)
                  cACCTCGGCCTGGTGACGCAAAATATCGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTg                                                                   <  2:3709250/93‑1 (MQ=255)
                   aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGc                                                                  >  2:158430/1‑93 (MQ=255)
                   aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGc                                                                  >  1:2538633/1‑93 (MQ=255)
                     cTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCaa                                                                <  1:2731995/93‑1 (MQ=255)
                     cTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCA‑CGGCG‑‑AATCCTCTTTGCaa                                                                <  2:4578636/93‑1 (MQ=255)
                      tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAAc                                                               >  1:3772551/1‑93 (MQ=255)
                      tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAAc                                                               >  1:3274908/1‑93 (MQ=255)
                        ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACtt                                                             <  2:2665299/93‑1 (MQ=255)
                        ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACtt                                                             <  2:4202152/93‑1 (MQ=255)
                         gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTtg                                                            >  1:1516186/1‑93 (MQ=255)
                         gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTtg                                                            >  2:2054614/1‑93 (MQ=255)
                         gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTtg                                                            >  1:1899526/1‑93 (MQ=255)
                         gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGAG‑‑AATCCTCTTTGAAACTtg                                                            >  2:740478/1‑93 (MQ=255)
                           cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  1:4438395/1‑93 (MQ=255)
                           cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  1:3828734/1‑93 (MQ=255)
                                 aCGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTtgccgtgccg                                                    <  1:2308603/93‑1 (MQ=255)
                                  cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgc                                                   >  2:11/1‑93 (MQ=255)
                                    cAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGt                                                 <  1:1556481/93‑1 (MQ=255)
                                      aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGTCGCGTAt                                               <  1:2318751/93‑1 (MQ=255)
                                      aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               >  2:3777329/1‑93 (MQ=255)
                                       aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              >  1:3062488/1‑93 (MQ=255)
                                       aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              <  2:2576013/93‑1 (MQ=255)
                                          atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           <  1:636805/93‑1 (MQ=255)
                                           ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          <  1:4103545/93‑1 (MQ=255)
                                            tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCATCTTGCCGTGCCGCGTATCGTGgc                                         <  2:790747/93‑1 (MQ=255)
                                            tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  2:2217808/93‑1 (MQ=255)
                                             gCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcg                                        >  2:2009476/1‑93 (MQ=255)
                                              cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcgc                                       >  1:2101561/1‑93 (MQ=255)
                                               ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  1:1090251/1‑93 (MQ=255)
                                                cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTc                                     >  2:2799823/1‑93 (MQ=255)
                                                  gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   >  1:268601/1‑93 (MQ=255)
                                                   cATTCTGCCATACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                  <  1:4257405/93‑1 (MQ=255)
                                                    aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCTGCGTATCGTGGCGCTCAAAc                                 <  2:2384219/93‑1 (MQ=255)
                                                    aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 <  2:4322019/93‑1 (MQ=255)
                                                    aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 >  1:285458/1‑93 (MQ=255)
                                                     ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                <  2:3179396/93‑1 (MQ=255)
                                                      tCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCCGC‑G‑‑AATCCTCTGTGCAACTTGCCGTGCCGCGTATCGTGACGCTCAAAcca                               >  2:38655/1‑92 (MQ=255)
                                                       cTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGa                              >  2:3083271/1‑93 (MQ=255)
                                                       cTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGa                              >  2:3072394/1‑93 (MQ=255)
                                                        tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             >  2:1446960/1‑93 (MQ=255)
                                                          cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  1:3536085/93‑1 (MQ=255)
                                                           ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  1:3410768/1‑93 (MQ=255)
                                                           ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  1:293277/1‑93 (MQ=255)
                                                           ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  2:2891754/1‑93 (MQ=255)
                                                           ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          <  1:1112549/93‑1 (MQ=255)
                                                            cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         >  2:1352725/1‑93 (MQ=255)
                                                             tACAACTGACAGCGGCCGCGCGCCCGGTGCTGCG‑CG‑‑GCAAATCCTCTTTGCAACTTGCCGTGCGGCGTATCGTGGCGCTCAAACCGAAAGCGa                        <  2:3353017/93‑1 (MQ=255)
                                                             tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  2:169488/1‑93 (MQ=255)
                                                             tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  1:705105/1‑93 (MQ=255)
                                                              aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       <  2:3896383/93‑1 (MQ=255)
                                                              aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       <  1:2360563/93‑1 (MQ=255)
                                                              aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       <  2:3448518/93‑1 (MQ=255)
                                                                aaCTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     <  2:587081/93‑1 (MQ=255)
                                                                aaCTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGACG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTAAAACCGAAAGCGATGc                     <  2:2036718/93‑1 (MQ=255)
                                                                aaCTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CG‑‑GCTAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     <  1:2305248/93‑1 (MQ=255)
                                                                 aCTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  1:3468117/1‑93 (MQ=255)
                                                                 aCTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  2:3524250/93‑1 (MQ=255)
                                                                 aCTGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  1:3908659/1‑93 (MQ=255)
                                                                   tGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGa                  <  1:3321294/93‑1 (MQ=255)
                                                                   tGACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGa                  >  2:869544/1‑93 (MQ=255)
                                                                    gACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                 >  2:3588946/1‑93 (MQ=255)
                                                                    gACAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                 >  2:24557/1‑93 (MQ=255)
                                                                     aCAGAGGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                >  2:1505923/1‑93 (MQ=255)
                                                                     aCAGAGGCCGCGCGCCCGGTCCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                <  2:1242535/93‑1 (MQ=255)
                                                                       agagGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              <  1:4357365/93‑1 (MQ=255)
                                                                       agagGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              >  1:4230880/1‑93 (MQ=255)
                                                                        gagGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATGGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             <  1:868770/93‑1 (MQ=255)
                                                                        gagGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             <  1:1972920/93‑1 (MQ=255)
                                                                         agGCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            <  2:2821227/93‑1 (MQ=255)
                                                                          ggCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  1:266656/93‑1 (MQ=255)
                                                                          ggCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           >  1:2592817/1‑93 (MQ=255)
                                                                          ggCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           >  1:1461741/1‑93 (MQ=255)
                                                                          ggCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  2:4529505/93‑1 (MQ=255)
                                                                           gCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  2:2517610/93‑1 (MQ=255)
                                                                           gCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          >  2:4069755/1‑93 (MQ=255)
                                                                           gCCGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  2:1167481/93‑1 (MQ=255)
                                                                           gCCCCGCGCACGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  1:1636489/93‑1 (MQ=255)
                                                                            ccGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         >  1:2497850/1‑93 (MQ=255)
                                                                            ccGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         <  1:3009913/93‑1 (MQ=255)
                                                                            ccGCGCGCCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         <  2:1887946/93‑1 (MQ=255)
                                                                              gcgcgcCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       <  2:3006694/93‑1 (MQ=255)
                                                                              gcgcgcCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:467985/1‑93 (MQ=255)
                                                                              gcgcgcCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       <  2:1436857/93‑1 (MQ=255)
                                                                              gcgcgcCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:2882959/1‑93 (MQ=255)
                                                                               cgcgcCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  1:1020940/93‑1 (MQ=255)
                                                                               cgcgcCCGGTGCTGCG‑CGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCACTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  1:599894/93‑1 (MQ=255)
                                                                                            |                                                                                      
GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CG‑CG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004888‑4005060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063
                                                                                              |                                                                                           
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T                                                                                             <  SRR4302535.2094846/94‑1 (MQ=60)
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T                                                                                             <  SRR4302535.3079672/94‑1 (MQ=60)
GCGTGATCCGCCAGCTGAGTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T                                                                                             <  SRR4302535.4656310/94‑1 (MQ=60)
 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑G                                                                                         >  SRR4302535.3032008/1‑94 (MQ=60)
  GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgc                                                                                           >  SRR4302535.3389613/1‑92 (MQ=60)
    GATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGC                                                                                       >  SRR4302535.3352978/1‑94 (MQ=60)
     ATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCG                                                                                      <  SRR4302535.3119442/94‑1 (MQ=60)
       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggc                                                                                      <  SRR4302535.2739917/94‑8 (MQ=60)
       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCG‑‑G‑‑A                                                                                <  SRR4302535.3335101/94‑1 (MQ=60)
       CCGCCAGATGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggc                                                                                      <  SRR4302535.2919092/94‑8 (MQ=60)
        CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcg                                                                                     >  SRR4302535.4030109/1‑86 (MQ=60)
         GCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcga                                                                                    >  SRR4302535.3674346/1‑85 (MQ=60)
          CCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaa                                                                                   <  SRR4302535.4687776/94‑11 (MQ=60)
          CCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaa                                                                                   >  SRR4302535.992559/1‑84 (MQ=60)
             GCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaatcc                                                                                <  SRR4302535.4698816/94‑14 (MQ=60)
             GCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaatcc                                                                                <  SRR4302535.3474506/94‑14 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCT                                                                           <  SRR4302535.1376601/94‑1 (MQ=60)
               TGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑Tgcgcggcgaatcctt                                                                              >  SRR4302535.2899266/1‑79 (MQ=60)
                TATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCT                                                                         <  SRR4302535.3998720/94‑1 (MQ=60)
                GATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCT                                                                         <  SRR4302535.4370362/94‑1 (MQ=60)
                 ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTT                                                                        <  SRR4302535.2704702/94‑1 (MQ=60)
                 ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTT                                                                        >  SRR4302535.2169075/1‑94 (MQ=60)
                   TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTG                                                                      <  SRR4302535.1845053/94‑1 (MQ=60)
                    CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGC                                                                     >  SRR4302535.2556921/1‑94 (MQ=60)
                     ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCA                                                                    >  SRR4302535.632779/1‑94 (MQ=60)
                      CCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAA                                                                   <  SRR4302535.607974/94‑1 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAAC                                                                  >  SRR4302535.3798572/1‑94 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAAC                                                                  <  SRR4302535.1888038/94‑1 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAAC                                                                  <  SRR4302535.2751499/94‑1 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAAC                                                                  >  SRR4302535.3297653/1‑94 (MQ=60)
                        TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACT                                                                 <  SRR4302535.3833381/94‑1 (MQ=60)
                          GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTG                                                               >  SRR4302535.1913196/1‑94 (MQ=60)
                          GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTG                                                               >  SRR4302535.1527107/1‑94 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             >  SRR4302535.2331260/1‑94 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             >  SRR4302535.4468816/1‑94 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             <  SRR4302535.1559629/94‑1 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             >  SRR4302535.3855143/1‑94 (MQ=60)
                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCG                                                            >  SRR4302535.3088037/1‑94 (MQ=60)
                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCG                                                            >  SRR4302535.2983430/1‑94 (MQ=60)
                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCG                                                            >  SRR4302535.1745097/1‑94 (MQ=60)
                              TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                           >  SRR4302535.44504/1‑94 (MQ=60)
                              TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                           <  SRR4302535.337830/94‑1 (MQ=60)
                                   ACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGC                                                      <  SRR4302535.2325127/94‑1 (MQ=60)
                                    CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCG                                                     >  SRR4302535.400174/1‑94 (MQ=60)
                                      CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                   <  SRR4302535.1567701/94‑1 (MQ=60)
                                      CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                   >  SRR4302535.1633853/1‑94 (MQ=60)
                                       AAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTAT                                                  >  SRR4302535.1077493/1‑94 (MQ=60)
                                        AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGTCGCGTATC                                                 <  SRR4302535.2335349/94‑1 (MQ=60)
                                        AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                 >  SRR4302535.3083900/1‑94 (MQ=60)
                                          ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               >  SRR4302535.804141/1‑94 (MQ=60)
                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             <  SRR4302535.641245/94‑1 (MQ=60)
                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             <  SRR4302535.1463548/94‑1 (MQ=60)
                                             TTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TGAGCGGC‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGC                                            <  SRR4302535.2530902/94‑1 (MQ=60)
                                             TTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGC                                            <  SRR4302535.4131639/94‑1 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           >  SRR4302535.2292711/1‑94 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           <  SRR4302535.2003256/94‑1 (MQ=60)
                                               GCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGC                                          >  SRR4302535.2116717/1‑94 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302535.1098021/1‑94 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         <  SRR4302535.2541041/94‑1 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302535.927924/1‑94 (MQ=60)
                                                 CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        >  SRR4302535.4699353/1‑94 (MQ=60)
                                                 CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        <  SRR4302535.1484656/94‑1 (MQ=60)
                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      >  SRR4302535.270469/1‑94 (MQ=60)
                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    >  SRR4302535.2324770/1‑94 (MQ=60)
                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    >  SRR4302535.287455/1‑94 (MQ=60)
                                                     CATTCTGCCATACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    <  SRR4302535.4286573/94‑1 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   <  SRR4302535.1100201/94‑1 (MQ=60)
                                                       TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAcatcg                                  >  SRR4302535.4353927/1‑89 (MQ=60)
                                                        TCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGA                                 >  SRR4302535.2510336/1‑94 (MQ=60)
                                                          TGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTT‑‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                               >  SRR4302535.4331030/1‑94 (MQ=60)
                                                          TGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                               >  SRR4302535.3534640/1‑94 (MQ=60)
                                                          TGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                               >  SRR4302535.3452773/1‑94 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              <  SRR4302535.3200640/94‑1 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             >  SRR4302535.3434499/1‑94 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             <  SRR4302535.3560632/94‑1 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             >  SRR4302535.295328/1‑94 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302535.3423461/1‑94 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            <  SRR4302535.1120486/94‑1 (MQ=60)
                                                              CTACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           >  SRR4302535.710054/1‑94 (MQ=60)
                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          <  SRR4302535.1945614/94‑1 (MQ=60)
                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAG                          >  SRR4302535.2569074/1‑94 (MQ=60)
                                                                ACAACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         <  SRR4302535.2377504/94‑1 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       >  SRR4302535.3492218/1‑94 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       <  SRR4302535.2676630/94‑1 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       <  SRR4302535.1603655/94‑1 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       >  SRR4302535.3935430/1‑94 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCG‑‑GCTAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       <  SRR4302535.2321738/94‑1 (MQ=60)
                                                                     TGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAA                    <  SRR4302535.3344403/94‑1 (MQ=60)
                                                                     TGACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAA                    <  SRR4302535.2150675/94‑1 (MQ=60)
                                                                       ACAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                  <  SRR4302535.2781920/94‑1 (MQ=60)
                                                                        CAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302535.3345139/94‑1 (MQ=60)
                                                                        CAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 >  SRR4302535.3605938/1‑94 (MQ=60)
                                                                        CAGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 >  SRR4302535.4259833/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                >  SRR4302535.4241097/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                <  SRR4302535.4387196/94‑1 (MQ=60)
                                                                          GAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATGGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT               <  SRR4302535.874917/94‑1 (MQ=60)
                                                                          GAGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT               <  SRR4302535.1987128/94‑1 (MQ=60)
                                                                           AGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              >  SRR4302535.2611444/1‑94 (MQ=60)
                                                                           AGGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              >  SRR4302535.1472215/1‑94 (MQ=60)
                                                                            GGCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             <  SRR4302535.268507/94‑1 (MQ=60)
                                                                             GCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            >  SRR4302535.2515869/1‑94 (MQ=60)
                                                                             GCCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302535.2377273/94‑1 (MQ=60)
                                                                             GCCCCGCGCACGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302535.1648312/94‑1 (MQ=60)
                                                                              CCGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           <  SRR4302535.3030996/94‑1 (MQ=60)
                                                                               CGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          >  SRR4302535.2920946/1‑94 (MQ=60)
                                                                               CGCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          >  SRR4302535.4215902/1‑94 (MQ=60)
                                                                                GGGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         <  SRR4302535.2319292/94‑1 (MQ=60)
                                                                                GCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         <  SRR4302535.4262252/94‑1 (MQ=60)
                                                                                GCGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         <  SRR4302535.705761/94‑1 (MQ=60)
                                                                                 CGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG        <  SRR4302535.1028195/94‑1 (MQ=60)
                                                                                 CGCGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCACTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG        <  SRR4302535.604091/94‑1 (MQ=60)
                                                                                   CGCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCA      <  SRR4302535.3866240/94‑1 (MQ=60)
                                                                                    GCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA     >  SRR4302535.4079843/1‑94 (MQ=60)
                                                                                    GCCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA     >  SRR4302535.4531209/1‑94 (MQ=60)
                                                                                     CCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    >  SRR4302535.1159565/1‑94 (MQ=60)
                                                                                     CCCGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAA    >  SRR4302535.1096636/1‑94 (MQ=60)
                                                                                       CGGTGC‑TG‑‑CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  SRR4302535.1275382/1‑94 (MQ=60)
                                                                                              |                                                                                           
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGC‑T‑‑‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑