Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
1873 |
183.1 |
9376148 |
98.2% |
9207377 |
93.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,008,404 |
+G |
coding (487/516 nt) |
b4555 → |
hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,008,402 | 1 | . | G | 100.0%
| 187.1
/ NA
| 59 | L162R (CTG→CGG) | b4555 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (25/34); total (25/34) |
TCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GC‑TGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTG > NC_000913/4008346‑4008458
|
tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGtt > 2:3254248/1‑93 (MQ=255)
tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGtt < 1:4432993/93‑1 (MQ=255)
tCTGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGtt < 1:2592961/93‑1 (MQ=255)
tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGtttg < 1:3793677/93‑3 (MQ=255)
tGGAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGtttg < 1:3632859/93‑3 (MQ=255)
ggAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGtttgg > 1:1240529/1‑90 (MQ=255)
gAAGTGATGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGtttggt < 1:1051163/93‑5 (MQ=255)
gAAGTGATGGCGGGCGGCGGTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGtttggt > 1:3702178/1‑89 (MQ=255)
aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACggg < 2:760103/93‑1 (MQ=255)
aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACggg < 2:229684/93‑1 (MQ=255)
aTGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACggg > 2:1077404/1‑93 (MQ=255)
tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGa < 1:1566229/93‑1 (MQ=255)
tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGa < 1:3374702/93‑1 (MQ=255)
tGGCGGGCGGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGa < 1:1894017/93‑1 (MQ=255)
ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGAt < 1:401856/93‑1 (MQ=255)
ggcgggcgGCGTTTCAAAAGGCCATGCGCTGGAAG‑CCGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGAt < 2:3408020/93‑1 (MQ=255)
gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATg < 2:1467147/93‑1 (MQ=255)
gcgggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATg < 1:3117785/93‑1 (MQ=255)
gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGaa < 1:1119358/93‑1 (MQ=255)
gggcggCGTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGaa < 1:4425676/93‑1 (MQ=255)
gcggcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacg > 1:4457103/1‑93 (MQ=255)
cggcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacga > 1:4576177/1‑93 (MQ=255)
cggcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacga < 2:3526130/93‑1 (MQ=255)
ggcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac > 2:516542/1‑93 (MQ=255)
ggcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac < 2:4322943/93‑1 (MQ=255)
ggcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac > 2:4523682/1‑93 (MQ=255)
ggcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac < 1:2958163/93‑1 (MQ=255)
ggcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgac < 1:2669860/93‑1 (MQ=255)
gcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgggg > 1:2074904/1‑90 (MQ=255)
gcgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAacgacg > 2:2058391/1‑93 (MQ=255)
cgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGc > 2:4150932/1‑93 (MQ=255)
cgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGc > 1:2192640/1‑93 (MQ=255)
cgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGc < 1:1265185/93‑1 (MQ=255)
cgTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGc < 2:1382101/93‑1 (MQ=255)
gTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGcc > 2:1671891/1‑93 (MQ=255)
gTTTCAAAAGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGcc < 2:1470272/93‑1 (MQ=255)
gTTTCAAAAGGCCATGCGCAGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGcc > 2:4571105/1‑93 (MQ=255)
canaaGNNCATGCGCTGGAAG‑NNGTGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa < 1:4559497/90‑1 (MQ=255)
caaaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa < 2:3847674/91‑1 (MQ=255)
caaaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGaaa < 1:2234580/91‑1 (MQ=255)
aaaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt > 2:1013108/2‑93 (MQ=255)
aaaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt > 1:2475525/2‑93 (MQ=255)
aaaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt > 2:2669759/2‑93 (MQ=255)
aaaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt > 1:4466518/2‑93 (MQ=255)
aaaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt < 2:4362651/92‑1 (MQ=255)
aaaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAAt > 1:2030356/2‑93 (MQ=255)
aaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg > 1:1050044/1‑93 (MQ=255)
aaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg > 2:470735/1‑93 (MQ=255)
aaaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATg > 1:4590471/1‑93 (MQ=255)
aaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGc < 1:4450069/93‑1 (MQ=255)
aaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGc < 1:2907437/93‑1 (MQ=255)
aaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGc > 1:3864513/1‑93 (MQ=255)
aaGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGc < 2:1078745/93‑1 (MQ=255)
aGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt > 2:1507239/1‑93 (MQ=255)
aGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt < 1:3115899/93‑1 (MQ=255)
aGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt < 1:4631955/93‑1 (MQ=255)
aGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt < 2:2559408/93‑1 (MQ=255)
aGGCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCt > 1:4654945/1‑93 (MQ=255)
ggCCATGCGCTGGAAGCGG‑TGGCGAAGAAACTGGGCTACAGCCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTg < 2:3964472/93‑1 (MQ=255)
|
TCTGGAAGTGATGGCGGGCGGCGTTTC‑AAAGGCCATGCGCTGGAAG‑GC‑TGGCG‑‑‑‑AACT‑GGCTACAG‑CTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTG > NC_000913/4008346‑4008458
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
