Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,014,125 G→A A36A (GCG→GCA)  udp → uridine phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,014,1250GA99.5% 768.6 / ‑5.9 216A36A (GCG→GCA) udpuridine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (99/116);  total (100/116)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 222 total aligned reads displayed.

TTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCGCTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTC  >  NC_000913/4014035‑4014214
                                                                                          |                                                                                         
 tttCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTg                                                                                        >  2:3358449/1‑93 (MQ=255)
 tttCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTg                                                                                        >  2:4635637/1‑93 (MQ=255)
  ttCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGa                                                                                       <  1:3609158/93‑1 (MQ=255)
  ttCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGa                                                                                       <  2:1613258/93‑1 (MQ=255)
  ttCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGa                                                                                       <  2:1741650/93‑1 (MQ=255)
   tCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGAt                                                                                      <  2:754999/93‑1 (MQ=255)
   tCATCGCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTCGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGAt                                                                                      <  2:740817/93‑1 (MQ=255)
     aTCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATgg                                                                                    <  1:2908826/93‑1 (MQ=255)
     aTCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATgg                                                                                    >  2:1784734/1‑93 (MQ=255)
      tctcGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGa                                                                                   >  1:2760895/1‑93 (MQ=255)
      tctcGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGa                                                                                   <  1:2079065/93‑1 (MQ=255)
       ccccgCCCCACCAAAAACGATTTACCAGGGGCCACCCCTGCCAACGGCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGAt                                                                                  <  1:1178556/89‑1 (MQ=255)
        tcGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATa                                                                                 >  1:3348103/1‑93 (MQ=255)
         cGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATaa                                                                                <  1:3207402/93‑1 (MQ=255)
           gCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGc                                                                              >  1:3166707/1‑93 (MQ=255)
            ccTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGcc                                                                             <  1:4323025/93‑1 (MQ=255)
              tCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCgg                                                                           <  2:2538919/93‑1 (MQ=255)
               cACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATGGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGt                                                                          <  2:3732665/93‑1 (MQ=255)
               cACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGt                                                                          >  1:4151698/1‑93 (MQ=255)
               cACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGt                                                                          <  1:1557045/93‑1 (MQ=255)
                aCTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGtt                                                                         <  1:3252147/93‑1 (MQ=255)
                aCTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGtt                                                                         <  2:1486457/93‑1 (MQ=255)
                aCTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGtt                                                                         >  1:3092694/1‑93 (MQ=255)
                  tAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTaa                                                                       <  2:3025016/93‑1 (MQ=255)
                  tAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTaa                                                                       <  1:953791/93‑1 (MQ=255)
                    aaaaCGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGc                                                                     <  2:2119498/93‑1 (MQ=255)
                    aaaaCGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGc                                                                     <  1:2545556/93‑1 (MQ=255)
                    aaaaCGATTTACAAGGGGCTACGCTGGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGc                                                                     <  2:1552838/93‑1 (MQ=255)
                     aaaCGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCt                                                                    <  1:1101756/93‑1 (MQ=255)
                       aCGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTgg                                                                  >  1:1546125/1‑93 (MQ=255)
                       aCGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTgg                                                                  <  2:4420475/93‑1 (MQ=255)
                         gATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGct                                                                >  2:3482905/1‑92 (MQ=255)
                         gATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCa                                                                >  1:2565013/1‑93 (MQ=255)
                           tttACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCAtc                                                              <  1:132590/93‑1 (MQ=255)
                           tttACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCAtc                                                              >  1:3916670/1‑93 (MQ=255)
                             tACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCAtctc                                                            >  1:1722853/1‑93 (MQ=255)
                              aCAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCa                                                           <  2:3707/93‑1 (MQ=255)
                              aCAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCa                                                           <  2:1861448/93‑1 (MQ=255)
                                aaGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCAcc                                                         <  2:2439666/93‑1 (MQ=255)
                                 aGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACcg                                                        >  2:260922/1‑93 (MQ=255)
                                 aGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGACCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAACTGGCATCTCACcg                                                        >  1:2493143/1‑93 (MQ=255)
                                   gggCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGGTAAGCTGGCATCTCACcgcg                                                      >  2:3639545/1‑93 (MQ=255)
                                       tACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAAtt                                                  <  1:4084334/93‑1 (MQ=255)
                                       tACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAAtt                                                  <  2:4016184/93‑1 (MQ=255)
                                       tACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAAtt                                                  >  2:4246015/1‑93 (MQ=255)
                                        aCGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTc                                                 >  2:3872697/1‑93 (MQ=255)
                                         cGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCa                                                >  1:1795551/1‑93 (MQ=255)
                                          gCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCAc                                               >  1:4439096/1‑93 (MQ=255)
                                            ttGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTa                                             >  2:4669719/1‑93 (MQ=255)
                                            ttGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACAGCGAATTCACTa                                             >  1:4210053/1‑93 (MQ=255)
                                             tGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTAc                                            <  2:555979/93‑1 (MQ=255)
                                             tGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTAc                                            <  2:2225289/93‑1 (MQ=255)
                                               ccATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCt                                          <  2:1044908/93‑1 (MQ=255)
                                                cATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTg                                         >  2:188976/1‑93 (MQ=255)
                                                 aTCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTgg                                        <  1:511623/93‑1 (MQ=255)
                                                 aTCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTgg                                        >  2:2164871/1‑93 (MQ=255)
                                                 aTCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTgg                                        >  2:2527233/1‑93 (MQ=255)
                                                  tCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGc                                       <  2:3099530/93‑1 (MQ=255)
                                                  tCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGc                                       <  2:1731907/93‑1 (MQ=255)
                                                    gTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGt                                     <  2:4381955/93‑1 (MQ=255)
                                                      cccTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGc                                   >  2:2946093/1‑93 (MQ=255)
                                                       ccTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCa                                  <  1:2687997/93‑1 (MQ=255)
                                                       ccTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCa                                  <  1:4544093/93‑1 (MQ=255)
                                                        cTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCag                                 >  2:229865/1‑93 (MQ=255)
                                                        cTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCag                                 >  1:2626778/1‑93 (MQ=255)
                                                         tGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCaga                                <  2:4197107/93‑1 (MQ=255)
                                                         tGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCaga                                <  1:1078560/93‑1 (MQ=255)
                                                         tGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCaga                                <  1:4641982/93‑1 (MQ=255)
                                                         tGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCaga                                >  1:694240/1‑93 (MQ=255)
                                                         tGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAAATCACTACCTGGCGTGCaga                                >  2:3212916/1‑93 (MQ=255)
                                                             gACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTg                            <  2:492693/93‑1 (MQ=255)
                                                              aCCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTgg                           >  1:3473062/1‑93 (MQ=255)
                                                               cccGGATCGTGTGTGAAAGATCGCCGGACTGATGGATAAGCCTGGTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGa                          <  1:851584/93‑1 (MQ=255)
                                                               cccGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGa                          <  2:409653/93‑1 (MQ=255)
                                                                ccGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGAt                         >  1:1045371/1‑93 (MQ=255)
                                                                ccGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGAt                         <  2:4132019/93‑1 (MQ=255)
                                                                 cGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGTGCTGGATg                        <  2:4574260/93‑1 (MQ=255)
                                                                 cGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATg                        >  2:265228/1‑93 (MQ=255)
                                                                 cGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATg                        >  1:369463/1‑93 (MQ=255)
                                                                  ggATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATgg                       >  2:1469260/1‑93 (MQ=255)
                                                                  ggATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATgg                       >  2:2466045/1‑93 (MQ=255)
                                                                  ggATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATgg                       >  2:2876290/1‑93 (MQ=255)
                                                                   gATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGt                      >  2:4118811/1‑93 (MQ=255)
                                                                   gATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGATGGATGGt                      >  2:857823/1‑93 (MQ=255)
                                                                     tCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTaa                    <  1:1950589/93‑1 (MQ=255)
                                                                     tCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTaa                    <  2:2795460/93‑1 (MQ=255)
                                                                      cGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTaaa                   <  1:2652535/93‑1 (MQ=255)
                                                                       gtgtGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAAc                  >  1:1694552/1‑93 (MQ=255)
                                                                        tgtgGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAAcc                 <  2:1987585/93‑1 (MQ=255)
                                                                        tgtgGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAAcc                 >  1:2024607/1‑93 (MQ=255)
                                                                        tgtgGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAAcc                 >  2:2703583/1‑93 (MQ=255)
                                                                          tgGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTg               <  1:3329271/93‑1 (MQ=255)
                                                                           ggAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGt              <  2:1317062/93‑1 (MQ=255)
                                                                            gAAAAGATCGCAGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGtt             <  2:3557307/93‑1 (MQ=255)
                                                                             aaaaGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTa            <  1:2276531/93‑1 (MQ=255)
                                                                             aaaaGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTa            >  2:2342576/1‑93 (MQ=255)
                                                                              aaaGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTAt           <  2:395900/93‑1 (MQ=255)
                                                                                  aTCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTc       <  2:614907/93‑1 (MQ=255)
                                                                                   tCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCt      <  1:3461349/93‑1 (MQ=255)
                                                                                       cgcaCTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGctc  <  1:3947077/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCGCTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTC  >  NC_000913/4014035‑4014214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCGCTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTCTACC  >  NC_000913/4014033‑4014218
                                                                                            |                                                                                             
GTTTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCAC                                                                                              >  SRR4302535.4581405/1‑94 (MQ=60)
GTTTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCAC                                                                                              >  SRR4302535.1635892/1‑94 (MQ=60)
 TTTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACT                                                                                             <  SRR4302535.3703573/94‑1 (MQ=60)
 TTTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACT                                                                                             >  SRR4302535.4343095/1‑94 (MQ=60)
  TTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTG                                                                                            <  SRR4302535.1456103/94‑1 (MQ=60)
    TTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGAT                                                                                          <  SRR4302535.891990/94‑1 (MQ=60)
    TTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGAT                                                                                          <  SRR4302535.3634191/94‑1 (MQ=60)
     TCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATG                                                                                         <  SRR4302535.2324494/94‑1 (MQ=60)
     TCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATG                                                                                         >  SRR4302535.1943347/1‑94 (MQ=60)
       ATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGA                                                                                       <  SRR4302535.2929383/94‑1 (MQ=60)
       ATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGA                                                                                       >  SRR4302535.2780569/1‑94 (MQ=60)
        TCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGAT                                                                                      <  SRR4302535.2094041/94‑1 (MQ=60)
         ccccgccccaccAAAAACGATTTACCAGGGGCCACCCCTGCCAACGGCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATA                                                                                     <  SRR4302535.1186962/82‑1 (MQ=55)
         CTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATA                                                                                     >  SRR4302535.3371426/1‑94 (MQ=60)
         CTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATA                                                                                     <  SRR4302535.2651199/94‑1 (MQ=60)
          TCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAA                                                                                    <  SRR4302535.155923/94‑1 (MQ=60)
           CGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAG                                                                                   <  SRR4302535.3229616/94‑1 (MQ=60)
            GGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGC                                                                                  >  SRR4302535.3188651/1‑94 (MQ=60)
            GGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGC                                                                                  >  SRR4302535.1711979/1‑94 (MQ=60)
             GCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCC                                                                                 <  SRR4302535.11499/94‑1 (MQ=60)
              CCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCG                                                                                <  SRR4302535.3359168/94‑1 (MQ=60)
              CCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCG                                                                                <  SRR4302535.4352599/94‑1 (MQ=60)
                TCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGT                                                                              >  SRR4302535.4180125/1‑94 (MQ=60)
                 CACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTT                                                                             >  SRR4302535.1901960/1‑94 (MQ=60)
                 CACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTT                                                                             <  SRR4302535.1568270/94‑1 (MQ=60)
                 CACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTT                                                                             >  SRR4302535.3114229/1‑94 (MQ=60)
                  ACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTA                                                                            <  SRR4302535.3274741/94‑1 (MQ=60)
                   CTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAA                                                                           >  SRR4302535.3203740/1‑94 (MQ=60)
                   CTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAA                                                                           >  SRR4302535.3749524/1‑94 (MQ=60)
                    TAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAG                                                                          <  SRR4302535.3457517/94‑1 (MQ=60)
                    TAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAG                                                                          <  SRR4302535.960548/94‑1 (MQ=60)
                     AAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGC                                                                         >  SRR4302535.3399336/1‑94 (MQ=60)
                      AAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCT                                                                        >  SRR4302535.3945295/1‑94 (MQ=60)
                      AAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCT                                                                        <  SRR4302535.2563881/94‑1 (MQ=60)
                       AAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTG                                                                       <  SRR4302535.1109631/94‑1 (MQ=60)
                        AACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGG                                                                      >  SRR4302535.1557261/1‑94 (MQ=60)
                          CGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCA                                                                    >  SRR4302535.2583473/1‑94 (MQ=60)
                          CGATTTACAAGGGGCTACGCTTGCCATCGGCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCA                                                                    <  SRR4302535.3129384/94‑1 (MQ=60)
                            ATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATC                                                                  >  SRR4302535.3943471/1‑94 (MQ=60)
                             TTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCT                                                                 <  SRR4302535.133520/94‑1 (MQ=60)
                              TTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTC                                                                >  SRR4302535.1735313/1‑94 (MQ=60)
                               TACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCA                                                               >  SRR4302535.1096685/1‑94 (MQ=60)
                                ACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTTAC                                                              >  SRR4302535.1230636/1‑94 (MQ=60)
                                 CAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATTGATAAGCCGGTTAAGCTGGCATCTCACC                                                             >  SRR4302535.4654295/1‑94 (MQ=60)
                                  AAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGACCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAACTGGCATCTCACCG                                                            >  SRR4302535.2511136/1‑94 (MQ=60)
                                       GCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCGCCGCGAAT                                                       <  SRR4302535.1999417/94‑1 (MQ=60)
                                         TACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTC                                                     <  SRR4302535.4112262/94‑1 (MQ=60)
                                          ACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCA                                                    >  SRR4302535.1808532/1‑94 (MQ=60)
                                          ACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCA                                                    >  SRR4302535.4116954/1‑94 (MQ=60)
                                           CGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCGCTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCAC                                                   >  SRR4302535.3188023/1‑94 (MQ=60)
                                           CGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCAC                                                   >  SRR4302535.4469524/1‑94 (MQ=60)
                                            GCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACT                                                  <  SRR4302535.496871/94‑1 (MQ=60)
                                            GCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACT                                                  >  SRR4302535.795572/1‑94 (MQ=60)
                                             CTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACAGCGAATTCACTA                                                 >  SRR4302535.4238872/1‑94 (MQ=60)
                                               TGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACC                                               >  SRR4302535.1664666/1‑94 (MQ=60)
                                                GCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCT                                              >  SRR4302535.2840021/1‑94 (MQ=60)
                                                 CCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTG                                             >  SRR4302535.25396/1‑94 (MQ=60)
                                                 CCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGAGCGCCGCACTGATGGATAAGCCGGTGAAGCTGGCATCTCACCGCGAATTCACTACCTG                                             <  SRR4302535.4709715/94‑1 (MQ=60)
                                                   ATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGC                                           >  SRR4302535.4260131/1‑94 (MQ=60)
                                                   ATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGC                                           >  SRR4302535.2689304/1‑94 (MQ=60)
                                                   ATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGC                                           <  SRR4302535.515198/94‑1 (MQ=60)
                                                        CCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCA                                      <  SRR4302535.2982474/94‑1 (MQ=60)
                                                         CCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAG                                     >  SRR4302535.3316846/1‑94 (MQ=60)
                                                         CCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAG                                     >  SRR4302535.4502247/1‑94 (MQ=60)
                                                         CCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAG                                     <  SRR4302535.4575170/94‑1 (MQ=60)
                                                         CCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAG                                     >  SRR4302535.2645601/1‑94 (MQ=60)
                                                         CCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAG                                     <  SRR4302535.2707208/94‑1 (MQ=60)
                                                          CTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGA                                    >  SRR4302535.699110/1‑94 (MQ=60)
                                                          CTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGA                                    <  SRR4302535.2573687/94‑1 (MQ=60)
                                                           TGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAG                                   <  SRR4302535.2076360/94‑1 (MQ=60)
                                                           TGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAG                                   <  SRR4302535.1086237/94‑1 (MQ=60)
                                                           TGGCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAG                                   <  SRR4302535.4673448/94‑1 (MQ=60)
                                                             GCGACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCT                                 <  SRR4302535.3749762/94‑1 (MQ=60)
                                                               GACCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGG                               >  SRR4302535.3497194/1‑94 (MQ=60)
                                                                 CCCGGATCGTGTGTGAAAGATCGCCGGACTGATGGATAAGCCTGGTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGAT                             <  SRR4302535.857581/94‑1 (MQ=60)
                                                                 CCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGAT                             <  SRR4302535.1800632/94‑1 (MQ=60)
                                                                 CCCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGAT                             >  SRR4302535.1052779/1‑94 (MQ=60)
                                                                  CCGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATG                            >  SRR4302535.372015/1‑94 (MQ=60)
                                                                   CGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGG                           >  SRR4302535.3495647/1‑94 (MQ=60)
                                                                   CGGATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGG                           >  SRR4302535.554049/1‑94 (MQ=60)
                                                                      ATCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAA                        >  SRR4302535.3704866/1‑94 (MQ=60)
                                                                       TCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAA                       <  SRR4302535.423302/94‑1 (MQ=60)
                                                                       TCGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAA                       <  SRR4302535.1964631/94‑1 (MQ=60)
                                                                       TCGTGTGAAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAA                       <  SRR4302535.1384319/94‑1 (MQ=60)
                                                                        CGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAAC                      <  SRR4302535.2671538/94‑1 (MQ=60)
                                                                        CGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAAC                      >  SRR4302535.1706805/1‑94 (MQ=60)
                                                                        CGTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGAAGAGCTGGGTGGTAAAC                      >  SRR4302535.2686357/1‑94 (MQ=60)
                                                                         GTGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACC                     >  SRR4302535.2039197/1‑94 (MQ=60)
                                                                          TGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCT                    <  SRR4302535.442964/94‑1 (MQ=60)
                                                                          TGTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCG                    >  SRR4302535.1053158/1‑94 (MQ=60)
                                                                           GTGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTG                   >  SRR4302535.2617597/1‑94 (MQ=60)
                                                                            TGGAAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGT                  <  SRR4302535.3352433/94‑1 (MQ=60)
                                                                               AAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTAT               <  SRR4302535.4066977/94‑1 (MQ=60)
                                                                               AAAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTAT               <  SRR4302535.2292787/94‑1 (MQ=60)
                                                                                AAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATC              <  SRR4302535.2417383/94‑1 (MQ=60)
                                                                                AAAGATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATC              >  SRR4302535.3501552/1‑94 (MQ=60)
                                                                                    ATCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCT          <  SRR4302535.3247177/94‑1 (MQ=60)
                                                                                    AGCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCT          <  SRR4302535.2715570/94‑1 (MQ=60)
                                                                                     TCGCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTG         <  SRR4302535.3485415/94‑1 (MQ=60)
                                                                                       GCCGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCT       <  SRR4302535.227005/94‑1 (MQ=60)
                                                                                         CGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTCT     <  SRR4302535.3974027/94‑1 (MQ=60)
                                                                                         CGCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTCT     >  SRR4302535.3405948/1‑94 (MQ=60)
                                                                                          GCACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTCTA    >  SRR4302535.2484220/1‑94 (MQ=60)
                                                                                           CACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTCTAC   <  SRR4302535.4697113/94‑1 (MQ=60)
                                                                                            ACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTCTACC  >  SRR4302535.747841/1‑94 (MQ=60)
                                                                                            ACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTCTACC  <  SRR4302535.3881656/94‑1 (MQ=60)
                                                                                            ACTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTCTACC  >  SRR4302535.2991903/1‑94 (MQ=60)
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GTTTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCTTGCCATCGTCCCTGGCGACCCGGATCGTGTGGAAAAGATCGCCGCGCTGATGGATAAGCCGGTTAAGCTGGCATCTCACCGCGAATTCACTACCTGGCGTGCAGAGCTGGATGGTAAACCTGTTATCGTCTGCTCTACC  >  NC_000913/4014033‑4014218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑