Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,023,531 T→C H318H (CAT→CAC)  yigC → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,023,5310TC99.6% 709.1 / ‑5.2 223H318H (CAT→CAC) yigCputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (136/86);  minor base A (0/1);  total (136/87)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 234 total aligned reads displayed.

TGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTT  >  NC_000913/4023441‑4023620
                                                                                          |                                                                                         
tGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTc                                                                                         >  1:1944337/1‑93 (MQ=255)
tGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGGATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTc                                                                                         >  1:3566140/1‑93 (MQ=255)
 ggCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTcc                                                                                        <  1:1908089/93‑1 (MQ=255)
     aCCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGGGAAGATGCGATTTACCATTCCActt                                                                                    >  2:687895/1‑91 (MQ=255)
      ccACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCta                                                                                   >  2:2693684/1‑93 (MQ=255)
       cacaCCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCtat                                                                                  >  2:1310363/1‑93 (MQ=255)
       cacaCCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCtat                                                                                  <  1:4401926/93‑1 (MQ=255)
        acacCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCtata                                                                                 >  1:441706/1‑93 (MQ=255)
          acCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAcc                                                                               <  2:2236159/93‑1 (MQ=255)
          acCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAcc                                                                               >  1:2818188/1‑93 (MQ=255)
           ccGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAGCg                                                                              >  2:3426656/1‑93 (MQ=255)
            cGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCgg                                                                             >  2:2299681/1‑93 (MQ=255)
            cGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCgg                                                                             <  2:4137711/93‑1 (MQ=255)
            cGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCgg                                                                             >  1:3078013/1‑93 (MQ=255)
              gTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGc                                                                           >  1:1601432/1‑93 (MQ=255)
              gTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGc                                                                           <  2:2152893/93‑1 (MQ=255)
               ttACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCg                                                                          <  2:2743542/93‑1 (MQ=255)
               ttACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCg                                                                          >  2:1791278/1‑93 (MQ=255)
                tACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGt                                                                         >  1:3133617/1‑93 (MQ=255)
                 aCTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTc                                                                        >  1:1254924/1‑93 (MQ=255)
                 aCTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTc                                                                        >  2:4258307/1‑93 (MQ=255)
                 aCTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTc                                                                        <  1:1432960/93‑1 (MQ=255)
                 aCTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTc                                                                        >  1:2765408/1‑93 (MQ=255)
                  cTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTcc                                                                       <  1:1518120/93‑1 (MQ=255)
                   tataATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGAGTCCg                                                                      >  2:1711841/1‑93 (MQ=255)
                    ataATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGc                                                                     >  2:3248671/1‑93 (MQ=255)
                         gAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGAt                                                                <  1:3612323/93‑1 (MQ=255)
                         gAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGAt                                                                >  1:3723352/1‑93 (MQ=255)
                          aaGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATg                                                               >  1:3676589/1‑93 (MQ=255)
                           aGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGa                                                              <  2:3606335/93‑1 (MQ=255)
                            gTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAg                                                             <  1:3205202/93‑1 (MQ=255)
                            gTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAg                                                             >  2:3560855/1‑93 (MQ=255)
                             tCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGc                                                            >  2:1717193/1‑93 (MQ=255)
                             tCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGc                                                            >  2:3720705/1‑93 (MQ=255)
                               gATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGccc                                                          >  2:1113775/1‑93 (MQ=255)
                               gATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGccc                                                          >  2:3372462/1‑93 (MQ=255)
                                aTAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCcg                                                         >  1:3599772/1‑93 (MQ=255)
                                aTAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCcg                                                         <  1:2011359/93‑1 (MQ=255)
                                   gTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCgg                                                      >  2:1647393/1‑93 (MQ=255)
                                      tCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGc                                                   <  1:1114410/93‑1 (MQ=255)
                                       cccGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCt                                                  >  1:2309011/1‑93 (MQ=255)
                                         cGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTgg                                                <  2:4465825/93‑1 (MQ=255)
                                          ggTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTggg                                               >  2:1970974/1‑93 (MQ=255)
                                           gTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgt                                              >  2:3400268/1‑93 (MQ=255)
                                           gTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgt                                              >  2:1959597/1‑93 (MQ=255)
                                           gTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgt                                              >  1:2148142/1‑93 (MQ=255)
                                            tATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgtg                                             >  1:2417331/1‑93 (MQ=255)
                                            tATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGCGCGTCCGCCAGATGAGCCCGCGGTGCTGGgtg                                             >  1:3749596/1‑93 (MQ=255)
                                              tttACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGgtgtg                                           >  2:927192/1‑92 (MQ=255)
                                              tttACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTc                                           >  2:3182317/1‑93 (MQ=255)
                                                     tGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGa                                    <  2:3953045/93‑1 (MQ=255)
                                                     tGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGa                                    >  1:490162/1‑93 (MQ=255)
                                                     tGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGa                                    <  2:4671922/93‑1 (MQ=255)
                                                      gACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGaa                                   >  2:4631634/1‑93 (MQ=255)
                                                       aCGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAAc                                  >  1:1582664/1‑93 (MQ=255)
                                                        cGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACg                                 <  1:909967/93‑1 (MQ=255)
                                                          cATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCCCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGaa                               >  1:2906853/1‑93 (MQ=255)
                                                            tatTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAgt                             <  2:849318/93‑1 (MQ=255)
                                                             atTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAgtg                            <  1:4636053/93‑1 (MQ=255)
                                                              ttACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAgtgt                           <  1:47250/93‑1 (MQ=255)
                                                               tACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGtt                          <  1:3878395/93‑1 (MQ=255)
                                                               tACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGtt                          <  1:4258442/93‑1 (MQ=255)
                                                                aCCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGttt                         >  1:1186414/1‑93 (MQ=255)
                                                                aCCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGttt                         >  2:1077516/1‑93 (MQ=255)
                                                                 cccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtg                        >  2:4059792/1‑93 (MQ=255)
                                                                 cccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtg                        >  2:2580657/1‑93 (MQ=255)
                                                                 cccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtg                        <  1:2631246/93‑1 (MQ=255)
                                                                  ccAGCGTGAAGATGCGATTTACCACTCCTCCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgt                       <  1:2977127/93‑1 (MQ=255)
                                                                  ccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgt                       >  1:1812460/1‑93 (MQ=255)
                                                                  ccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgt                       >  2:4327968/1‑93 (MQ=255)
                                                                  ccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgt                       >  2:29227/1‑93 (MQ=255)
                                                                  ccAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgt                       >  2:1210303/1‑93 (MQ=255)
                                                                  ccAGCGTGAAGATGCGATTTACAACTCCACCTATACCGGGCGTCCGCCAGATGAGCACGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTtgt                       >  1:746341/1‑93 (MQ=255)
                                                                    aGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGc                     >  1:2364589/1‑93 (MQ=255)
                                                                     gCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGcc                    <  1:669233/93‑1 (MQ=255)
                                                                       gTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGa                  >  1:3928075/1‑93 (MQ=255)
                                                                        tGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAt                 >  2:3917637/1‑93 (MQ=255)
                                                                         gAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAtt                >  1:3216781/1‑93 (MQ=255)
                                                                          aaGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTc               <  2:4595706/93‑1 (MQ=255)
                                                                          aaGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTc               <  1:102897/93‑1 (MQ=255)
                                                                          aaGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTc               <  2:14548/93‑1 (MQ=255)
                                                                          aaGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTc               >  1:4149973/1‑93 (MQ=255)
                                                                          aaGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTc               <  1:4317491/93‑1 (MQ=255)
                                                                           aGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCt              >  2:1162840/1‑93 (MQ=255)
                                                                           aGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCt              >  2:2544891/1‑93 (MQ=255)
                                                                           aGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACAAAGTGTTTGTGCCGATTCt              <  2:4562033/93‑1 (MQ=255)
                                                                             aTGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGc            >  2:4367420/1‑93 (MQ=255)
                                                                             aTGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGc            <  2:2061455/93‑1 (MQ=255)
                                                                              tGCGATTTACCACGCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCa           <  2:3063301/93‑1 (MQ=255)
                                                                                 gATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCaaaa        >  2:1432864/1‑93 (MQ=255)
                                                                                 gATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGGGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCaaaa        >  2:559825/1‑93 (MQ=255)
                                                                                  aTTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCaaaaa       >  1:4297856/1‑93 (MQ=255)
                                                                                   tttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAAc      <  1:1829690/93‑1 (MQ=255)
                                                                                   tttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAAc      <  1:546112/93‑1 (MQ=255)
                                                                                    ttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACa     <  2:2636519/93‑1 (MQ=255)
                                                                                    ttACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACa     >  1:4543473/1‑93 (MQ=255)
                                                                                     tgccACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAg    <  1:4473924/91‑1 (MQ=255)
                                                                                     tACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAg    <  1:3999810/93‑1 (MQ=255)
                                                                                      aCCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGt   <  2:4146176/93‑1 (MQ=255)
                                                                                       ccACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGtt  >  1:1238201/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTT  >  NC_000913/4023441‑4023620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  >  NC_000913/4023438‑4023624
                                                                                             |                                                                                             
GTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCAC                                                                                               <  SRR4302535.1317132/94‑1 (MQ=60)
 TATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACT                                                                                              <  SRR4302535.997026/94‑1 (MQ=60)
  ATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTC                                                                                             >  SRR4302535.4340357/1‑94 (MQ=60)
  ATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTC                                                                                             >  SRR4302535.1958337/1‑94 (MQ=60)
  ATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGGATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTC                                                                                             >  SRR4302535.3590865/1‑94 (MQ=60)
    GGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCA                                                                                           <  SRR4302535.2906782/94‑1 (MQ=60)
    GGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCA                                                                                           <  SRR4302535.1921846/94‑1 (MQ=60)
          CACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATA                                                                                     >  SRR4302535.444800/1‑94 (MQ=60)
          CACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATA                                                                                     <  SRR4302535.4432048/94‑1 (MQ=60)
          CACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATA                                                                                     >  SRR4302535.3041810/1‑94 (MQ=60)
           ACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATAC                                                                                    <  SRR4302535.538192/94‑1 (MQ=60)
            CACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACC                                                                                   >  SRR4302535.2838206/1‑94 (MQ=60)
             ACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCG                                                                                  >  SRR4302535.432937/1‑94 (MQ=60)
              CCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGG                                                                                 <  SRR4302535.1134619/94‑1 (MQ=60)
              CCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGG                                                                                 >  SRR4302535.3099482/1‑94 (MQ=60)
              CCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGG                                                                                 <  SRR4302535.4568877/94‑1 (MQ=60)
              CCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGG                                                                                 <  SRR4302535.4457251/94‑1 (MQ=60)
               CGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGG                                                                                >  SRR4302535.3110749/1‑94 (MQ=60)
                GGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGC                                                                               >  SRR4302535.1612985/1‑94 (MQ=60)
                GGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGC                                                                               >  SRR4302535.4681257/1‑94 (MQ=60)
                 GTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCG                                                                              >  SRR4302535.3967747/1‑94 (MQ=60)
                 GTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCG                                                                              >  SRR4302535.3690073/1‑94 (MQ=60)
                  TTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGT                                                                             >  SRR4302535.3155436/1‑94 (MQ=60)
                   TACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTC                                                                            >  SRR4302535.1263897/1‑94 (MQ=60)
                   TACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTC                                                                            >  SRR4302535.2785104/1‑94 (MQ=60)
                    ACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCC                                                                           <  SRR4302535.1849128/94‑1 (MQ=60)
                    ACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCC                                                                           <  SRR4302535.1443190/94‑1 (MQ=60)
                     CTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCG                                                                          <  SRR4302535.1529054/94‑1 (MQ=60)
                       ATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCC                                                                        >  SRR4302535.3611745/1‑94 (MQ=60)
                         AATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAG                                                                      >  SRR4302535.3100585/1‑94 (MQ=60)
                           TGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGAT                                                                    >  SRR4302535.3749145/1‑94 (MQ=60)
                            GAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATG                                                                   <  SRR4302535.3637371/94‑1 (MQ=60)
                            GAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATG                                                                   >  SRR4302535.3702042/1‑94 (MQ=60)
                             AAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGA                                                                  >  SRR4302535.3231139/1‑94 (MQ=60)
                             AAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGA                                                                  >  SRR4302535.490199/1‑94 (MQ=60)
                              AGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAG                                                                 >  SRR4302535.692177/1‑94 (MQ=60)
                               GTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGC                                                                >  SRR4302535.4456494/1‑94 (MQ=60)
                               GTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGC                                                                <  SRR4302535.3227399/94‑1 (MQ=60)
                                 CGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCC                                                              >  SRR4302535.812373/1‑94 (MQ=60)
                                  GATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCG                                                             >  SRR4302535.4300170/1‑94 (MQ=60)
                                  GATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCG                                                             >  SRR4302535.3624754/1‑94 (MQ=60)
                                  GATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCG                                                             >  SRR4302535.3624755/1‑94 (MQ=60)
                                   ATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGC                                                            >  SRR4302535.1946880/1‑94 (MQ=60)
                                   ATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGC                                                            <  SRR4302535.2025853/94‑1 (MQ=60)
                                    TAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCG                                                           <  SRR4302535.4713341/94‑1 (MQ=60)
                                      GTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGT                                                         >  SRR4302535.2293934/1‑94 (MQ=60)
                                      GTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGT                                                         >  SRR4302535.1242417/1‑94 (MQ=60)
                                      GTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGT                                                         <  SRR4302535.3645907/94‑1 (MQ=60)
                                      GTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGT                                                         >  SRR4302535.1742601/1‑94 (MQ=60)
                                      GTTTCCCGGTAATTACCGTGACGCATATTGCCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGT                                                         <  SRR4302535.2449968/94‑1 (MQ=60)
                                         TCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCT                                                      >  SRR4302535.4404377/1‑94 (MQ=60)
                                         TCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCT                                                      >  SRR4302535.2325540/1‑94 (MQ=60)
                                         TCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCT                                                      <  SRR4302535.1122354/94‑1 (MQ=60)
                                         TCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCT                                                      >  SRR4302535.3899097/1‑94 (MQ=60)
                                         TCCCGCTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCT                                                      <  SRR4302535.4441392/94‑1 (MQ=60)
                                          CCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTG                                                     >  SRR4302535.1350962/1‑94 (MQ=60)
                                           CCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGG                                                    >  SRR4302535.2181013/1‑94 (MQ=60)
                                            CGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGG                                                   <  SRR4302535.1659880/94‑1 (MQ=60)
                                             GGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGT                                                  <  SRR4302535.2929636/94‑1 (MQ=60)
                                             GGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGT                                                  >  SRR4302535.2163591/1‑94 (MQ=60)
                                              GTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCTACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTG                                                 <  SRR4302535.3317859/94‑1 (MQ=60)
                                              GTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTG                                                 >  SRR4302535.2434731/1‑94 (MQ=60)
                                              GTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTG                                                 <  SRR4302535.3203182/94‑1 (MQ=60)
                                              GTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGCGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTG                                                 >  SRR4302535.3775536/1‑94 (MQ=60)
                                               TATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGT                                                >  SRR4302535.183384/1‑94 (MQ=60)
                                                     CCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACT                                          >  SRR4302535.2785784/1‑94 (MQ=60)
                                                      CGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTG                                         >  SRR4302535.2021232/1‑94 (MQ=60)
                                                       GTGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGA                                        >  SRR4302535.493603/1‑94 (MQ=60)
                                                        TGACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAA                                       >  SRR4302535.80350/1‑94 (MQ=60)
                                                         GACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAAC                                      >  SRR4302535.1537439/1‑94 (MQ=60)
                                                         GACGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAAC                                      >  SRR4302535.1594087/1‑94 (MQ=60)
                                                           CGCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGA                                    <  SRR4302535.916433/94‑1 (MQ=60)
                                                            GCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAA                                   >  SRR4302535.3403366/1‑94 (MQ=60)
                                                            GCATATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCCCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAA                                   >  SRR4302535.2927398/1‑94 (MQ=60)
                                                               TATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTG                                >  SRR4302535.1102255/1‑94 (MQ=60)
                                                                ATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGT                               >  SRR4302535.463861/1‑94 (MQ=60)
                                                                ATTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGT                               <  SRR4302535.4667498/94‑1 (MQ=60)
                                                                 TTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTT                              <  SRR4302535.47605/94‑1 (MQ=60)
                                                                 TTACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTT                              <  SRR4302535.2870611/94‑1 (MQ=60)
                                                                  TACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTT                             <  SRR4302535.2372533/94‑1 (MQ=60)
                                                                  TACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTT                             <  SRR4302535.4287612/94‑1 (MQ=60)
                                                                  TACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTT                             <  SRR4302535.3905025/94‑1 (MQ=60)
                                                                  TACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTT                             >  SRR4302535.1194889/1‑94 (MQ=60)
                                                                   ACCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTG                            >  SRR4302535.3752365/1‑94 (MQ=60)
                                                                    CCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGT                           <  SRR4302535.2650097/94‑1 (MQ=60)
                                                                    CCCAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGT                           >  SRR4302535.1825568/1‑94 (MQ=60)
                                                                    CCCAGCGTGAAGATGCGATTTACAACTCCACCTATACCGGGCGTCCGCCAGATGAGCACGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGT                           >  SRR4302535.751590/1‑94 (MQ=60)
                                                                     CCAGCGTGAAGATGCGATTTACCACTCCTCCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTG                          <  SRR4302535.2998013/94‑1 (MQ=60)
                                                                      CAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGC                         >  SRR4302535.2381565/1‑94 (MQ=60)
                                                                      CAGCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGCCGCTCTGAACGAAGTGGTTGTGC                         >  SRR4302535.2388165/1‑94 (MQ=60)
                                                                        GCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCG                       >  SRR4302535.2036902/1‑94 (MQ=60)
                                                                        GCGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCG                       <  SRR4302535.673904/94‑1 (MQ=60)
                                                                         CGTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGA                      >  SRR4302535.3954906/1‑94 (MQ=60)
                                                                          GTGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGAT                     <  SRR4302535.1945041/94‑1 (MQ=60)
                                                                           TGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATT                    >  SRR4302535.550401/1‑94 (MQ=60)
                                                                           TGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATT                    >  SRR4302535.3239064/1‑94 (MQ=60)
                                                                           TGAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATT                    >  SRR4302535.1199745/1‑94 (MQ=60)
                                                                            GAAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTC                   >  SRR4302535.4178390/1‑94 (MQ=60)
                                                                             AAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCT                  <  SRR4302535.4096290/94‑1 (MQ=60)
                                                                             AAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCT                  <  SRR4302535.103625/94‑1 (MQ=60)
                                                                             AAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCT                  <  SRR4302535.3882373/94‑1 (MQ=60)
                                                                             AAGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCT                  <  SRR4302535.4347028/94‑1 (MQ=60)
                                                                              AGATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTG                 >  SRR4302535.1610573/1‑94 (MQ=60)
                                                                                ATGCGATTTACCCCTCCACCTATCCCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCA               <  SRR4302535.1255033/94‑1 (MQ=60)
                                                                                ATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCA               <  SRR4302535.150707/94‑1 (MQ=60)
                                                                                ATGCGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCA               <  SRR4302535.2553304/94‑1 (MQ=60)
                                                                                ATGCGATTTACCAATCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCA               <  SRR4302535.2698112/94‑1 (MQ=60)
                                                                                   CGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGGCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGGTTGTGCCGATTCTGCAAAA            >  SRR4302535.4165110/1‑94 (MQ=60)
                                                                                   CGATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGAGTTTGTGCCGATTCTGCAAAA            >  SRR4302535.3747581/1‑94 (MQ=60)
                                                                                    GATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAA           >  SRR4302535.4327268/1‑94 (MQ=60)
                                                                                    GATTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAA           >  SRR4302535.3934547/1‑94 (MQ=60)
                                                                                      TTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACA         >  SRR4302535.4574548/1‑94 (MQ=60)
                                                                                      TTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACA         <  SRR4302535.1842901/94‑1 (MQ=60)
                                                                                      TTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACA         <  SRR4302535.1525529/94‑1 (MQ=60)
                                                                                      TTTACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACA         <  SRR4302535.549930/94‑1 (MQ=60)
                                                                                        TGCCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGT       <  SRR4302535.4504623/94‑1 (MQ=60)
                                                                                        TACCACTCCACCTATCCCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGT       <  SRR4302535.2551715/94‑1 (MQ=60)
                                                                                        TACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGT       <  SRR4302535.4027174/94‑1 (MQ=60)
                                                                                        TACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGT       <  SRR4302535.563672/94‑1 (MQ=60)
                                                                                         ACCACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTT      >  SRR4302535.1247043/1‑94 (MQ=60)
                                                                                           CACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAGCGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCC    <  SRR4302535.464759/94‑1 (MQ=60)
                                                                                           CACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCC    >  SRR4302535.997873/1‑94 (MQ=60)
                                                                                           CACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCC    >  SRR4302535.4510489/1‑94 (MQ=60)
                                                                                            ACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCC   >  SRR4302535.2853297/1‑94 (MQ=60)
                                                                                            ACTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCC   >  SRR4302535.3049/1‑94 (MQ=60)
                                                                                             CTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  >  SRR4302535.2844757/1‑94 (MQ=60)
                                                                                             CTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  >  SRR4302535.3095914/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTTGTGCCGATTCTGCAAAAACAGTTCCCG  >  NC_000913/4023438‑4023624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑